MD15G1104100.v1.1

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010804
Physical Location & Seq
Reverse (-)
7323453 .. 7326288
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1104100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGCACATGATACAAAAATCCATACCACATACATCACTGACAACTCTAGCGCACGTGGCAAATCACGCGCTGCACACCCACGCGGGTTTTCGCTTCCCCTCGATATATAAATACGGTTGGCTGTCGCCTTCCTCCTTCCTCACCCAGCGAAACCCAGAAGAGAAAGAAAGAGTAGGCGAGGAGAGAGAAAGAGAGAGACAGACGAACCAGAAGAGAGAAACGCGGCGTTTAGCTTCATTCTCTTTTCGTTTCTCTTTTTCCTTGCCACTGACCACCACCGTCGATTTCTTTTCGCTTTCTCGATCTATCGAATCAGACCGCCCCAGGGGTTTCTTCAAACGCTTCATCTCGTTTTATTTTCTCGGAAAATTTCTGCGTTCTTCGTTGAAAGCTCCCGATCCCTCTTCAAACGCAGCCATGGTATTCTGGGTCTTTGGATATGGTTCACTTGTGTGGAACCCAGGATTCGAGTATGACGAGAAAGTGATAGGTTACATCAAGAACTACAGGCGTGTGTTTGATCTTGCATGCATTGATCACAGAGGTACACCTGAGCACCCTGCTCGGACTTGCACTTTGGAGTACCAAGAAGGAGCTGTCTGCTGGGGTGCTGCTTATTCTGTTCGTGGAGGGCCAGAAAGGGAGAAATTAGCAATGGAGTACTTGGAAAGGAGAGAATGTGAATATGATTCAAAGACTCTCGTCGACTTTTACAAGGAAGGGGAGTTCTCTGAGACTGAGCCTTCCTTGACAGGAGTTATTGTTTTCACGTCCACTCCAGACAAAGAATCGAACAAGTATTACCTTGGTCCTGCTCCATTGGAGGACATGGCAAGGCAAATTGCAACAGCTTTTGGGCCCTGCGGAAACAATAGAGACTATCTCTTTCTGTTGGAGAAGGCTATGTTTGATATAGGCCATGAAGAGGACTACGTGATAGAGCTAGCAAATGAGGTGAGGAAGGTTCTTGGAATAGCCGGAAAAGGATTTCCAAAGGAGAAGAAGTTGTCCGGGCCAACCCACCTCCCACTCAAGTCCAACATCCCGGCCCTCCAACTGCATCCGCTGCCGGAAGCCATCGCCATGGACTCGTAA

Protein Analysis

365

Amino Acids

41.68

Weight (kDa)

7.18

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 143 - 321 3.1e-43 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 705
AccII CGCG 3 cut(s) 68, 83, 223
AciI CCGC 5 cut(s) 83, 223, 319, 864, 1064
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 1 cut(s) 368
AcvI CACGTG 1 cut(s) 55
AfaI GTAC 3 cut(s) 547, 584, 662
AfiI CCNNNNNNNGG 2 cut(s) 325, 925
AgsI TTSAA 4 cut(s) 337, 388, 408, 693
AjiI CACGTC 1 cut(s) 771
AjnI CCWGG 2 cut(s) 323, 460
AleI CACNNNNGTG 1 cut(s) 451
AloI GAACNNNNNNTCC 2 cut(s) 710, 742
AluBI AGCT 5 cut(s) 233, 392, 596, 851, 943
AluI AGCT 5 cut(s) 233, 392, 596, 851, 943
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 3 cut(s) 190, 728, 870
AlwI GGATC 1 cut(s) 392
AoxI GGCC 5 cut(s) 632, 857, 916, 1013, 1047
ApaI GGGCCC 1 cut(s) 861
ApeKI GCWGC 4 cut(s) 70, 413, 611, 1066
ApoI RAATTY 1 cut(s) 368
ArsI GACNNNNNNTTYG 2 cut(s) 559, 591
AspLEI GCGC 2 cut(s) 52, 70
AspS9I GGNCC 6 cut(s) 632, 809, 857, 858, 1013, 1048
AsuC2I CCSGG 2 cut(s) 1012, 1046
AsuHPI GGTGA 2 cut(s) 133, 967
AsuNHI GCTAGC 1 cut(s) 943
AvaII GGWCC 1 cut(s) 809
BaeGI GKGCMC 1 cut(s) 861
BanII GRGCYC 1 cut(s) 861
BbrPI CACGTG 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 4 cut(s) 57, 425, 598, 1053
BccI CCATC 1 cut(s) 1085
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 2 cut(s) 325, 462
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 1012, 1046
BcoDI GTCTC 3 cut(s) 190, 728, 870
BfaI CTAG 2 cut(s) 47, 944
BfmI CTRYAG 1 cut(s) 505
BisI GCNGC 5 cut(s) 71, 224, 414, 612, 1067
BlsI GCNGC 5 cut(s) 72, 225, 415, 613, 1068
BmcAI AGTACT 1 cut(s) 662
Bme1390I CCNGG 4 cut(s) 325, 462, 1012, 1046
Bme18I GGWCC 1 cut(s) 809
BmgBI CACGTC 1 cut(s) 771
BmgT120I GGNCC 6 cut(s) 632, 809, 857, 858, 1013, 1048
BmiI GGNNCC 2 cut(s) 458, 859
BmrFI CCNGG 4 cut(s) 325, 462, 1012, 1046
BmsI GCATC 1 cut(s) 1069
BmtI GCTAGC 1 cut(s) 947
BoxI GACNNNNGTC 1 cut(s) 701
BplI GAGNNNNNCTC 2 cut(s) 867, 899
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 1016
BpuMI CCSGG 2 cut(s) 1012, 1046
BsaAI YACGTR 2 cut(s) 55, 934
BsaJI CCNNGG 6 cut(s) 323, 324, 417, 460, 805, 1083
BsaXI ACNNNNNCTCC 8 cut(s) 664, 694, 747, 760, 777, 790, 989, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 925
Bse3DI GCAATG 1 cut(s) 660
BseBI CCWGG 2 cut(s) 325, 462
BseDI CCNNGG 6 cut(s) 323, 324, 417, 460, 805, 1083
BseGI GGATG 2 cut(s) 1041, 1060
BseLI CCNNNNNNNGG 2 cut(s) 325, 925
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 3 cut(s) 543, 723, 729
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 1 cut(s) 861
BseXI GCAGC 4 cut(s) 57, 425, 598, 1053
BseYI CCCAGC 2 cut(s) 144, 603
BsgI GTGCAG 1 cut(s) 56
Bsh1236I CGCG 3 cut(s) 68, 83, 223
BshFI GGCC 5 cut(s) 634, 859, 918, 1015, 1049
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 4 cut(s) 978, 1011, 1046, 1070
BslI CCNNNNNNNGG 2 cut(s) 325, 925
BsmAI GTCTC 3 cut(s) 190, 728, 870
BsnI GGCC 5 cut(s) 634, 859, 918, 1015, 1049
Bsp120I GGGCCC 1 cut(s) 857
Bsp1286I GDGCHC 2 cut(s) 558, 861
Bsp143I GATC 4 cut(s) 302, 397, 520, 535
Bsp19I CCATGG 2 cut(s) 417, 1083
BspACI CCGC 5 cut(s) 83, 223, 319, 864, 1064
BspANI GGCC 5 cut(s) 634, 859, 918, 1015, 1049
BspCNI CTCAG 3 cut(s) 544, 724, 730
BspFNI CGCG 3 cut(s) 68, 83, 223
BspLI GGNNCC 2 cut(s) 458, 859
BspOI GCTAGC 1 cut(s) 947
BspPI GGATC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 660
BssECI CCNNGG 6 cut(s) 323, 324, 417, 460, 805, 1083
BssMI GATC 4 cut(s) 302, 397, 520, 535
BssT1I CCWWGG 3 cut(s) 417, 805, 1083
Bst2UI CCWGG 2 cut(s) 325, 462
Bst4CI ACNGT 2 cut(s) 116, 280
Bst6I CTCTTC 4 cut(s) 153, 206, 409, 918
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstBAI YACGTR 2 cut(s) 55, 934
BstC8I GCNNGC 2 cut(s) 529, 945
BstDEI CTNAG 3 cut(s) 552, 732, 738
BstDSI CCRYGG 2 cut(s) 417, 1083
BstF5I GGATG 2 cut(s) 1041, 1060
BstFNI CGCG 3 cut(s) 68, 83, 223
BstHHI GCGC 2 cut(s) 52, 70
BstKTI GATC 4 cut(s) 305, 400, 523, 538
BstMAI GTCTC 3 cut(s) 190, 728, 870
BstMBI GATC 4 cut(s) 302, 397, 520, 535
BstMWI GCNNNNNNNGC 3 cut(s) 56, 65, 1066
BstNI CCWGG 2 cut(s) 325, 462
BstNSI RCATGY 1 cut(s) 531
BstPAI GACNNNNGTC 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 323, 460, 1010, 1044
BstSFI CTRYAG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 861
BstUI CGCG 3 cut(s) 68, 83, 223
BstV1I GCAGC 4 cut(s) 57, 425, 598, 1053
BstXI CCANNNNNNTGG 1 cut(s) 1084
BsuRI GGCC 5 cut(s) 634, 859, 918, 1015, 1049
BtgI CCRYGG 2 cut(s) 417, 1083
BtgZI GCGATG 1 cut(s) 1063
BtrI CACGTC 1 cut(s) 771
BtsCI GGATG 2 cut(s) 1041, 1060
BtsIMutI CAGTG 2 cut(s) 35, 266
Cac8I GCNNGC 2 cut(s) 529, 945
CfoI GCGC 2 cut(s) 52, 70
Cfr13I GGNCC 6 cut(s) 632, 809, 857, 858, 1013, 1048
Csp6I GTAC 3 cut(s) 546, 583, 661
CviAII CATG 6 cut(s) 7, 418, 528, 829, 920, 1084
CviQI GTAC 3 cut(s) 546, 583, 661
DdeI CTNAG 3 cut(s) 552, 732, 738
DpnI GATC 4 cut(s) 304, 399, 522, 537
DpnII GATC 4 cut(s) 302, 397, 520, 535
Eam1104I CTCTTC 4 cut(s) 153, 206, 409, 918
EarI CTCTTC 4 cut(s) 153, 206, 409, 918
Eco130I CCWWGG 3 cut(s) 417, 805, 1083
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 1 cut(s) 809
Eco72I CACGTG 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 858
EcoRII CCWGG 2 cut(s) 323, 460
EcoT14I CCWWGG 3 cut(s) 417, 805, 1083
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 3 cut(s) 417, 805, 1083
FaeI CATG 6 cut(s) 10, 421, 531, 832, 923, 1087
FatI CATG 6 cut(s) 6, 417, 527, 828, 919, 1083
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 5 cut(s) 71, 224, 414, 612, 1067
FokI GGATG 2 cut(s) 1028, 1047
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 5 cut(s) 71, 224, 414, 612, 1067
FspBI CTAG 2 cut(s) 47, 944
GlaI GCGC 2 cut(s) 51, 69
GluI GCNGC 5 cut(s) 71, 224, 414, 612, 1067
GsaI CCCAGC 2 cut(s) 148, 607
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 5 cut(s) 634, 859, 918, 1015, 1049
HapII CCGG 4 cut(s) 978, 1011, 1046, 1070
HhaI GCGC 2 cut(s) 52, 70
Hin1II CATG 6 cut(s) 10, 421, 531, 832, 923, 1087
Hin6I GCGC 2 cut(s) 50, 68
HinP1I GCGC 2 cut(s) 50, 68
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 6 cut(s) 311, 465, 689, 697, 788, 1088
HpaII CCGG 4 cut(s) 978, 1011, 1046, 1070
HphI GGTGA 2 cut(s) 133, 967
Hpy166II GTNNAC 4 cut(s) 446, 548, 706, 774
Hpy188I TCNGA 4 cut(s) 316, 365, 567, 733
Hpy188III TCNNGA 4 cut(s) 300, 395, 499, 779
Hpy8I GTNNAC 4 cut(s) 446, 548, 706, 774
Hpy99I CGWCG 2 cut(s) 284, 707
HpyAV CCTTC 7 cut(s) 138, 145, 584, 713, 753, 892, 955
HpyCH4III ACNGT 2 cut(s) 116, 280
HpyCH4IV ACGT 3 cut(s) 54, 770, 933
HpyCH4V TGCA 7 cut(s) 4, 73, 527, 531, 573, 845, 1060
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 65, 1066
HpyF3I CTNAG 3 cut(s) 552, 732, 738
HpySE526I ACGT 3 cut(s) 54, 770, 933
Hsp92II CATG 6 cut(s) 10, 421, 531, 832, 923, 1087
HspAI GCGC 2 cut(s) 50, 68
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 4 cut(s) 302, 397, 520, 535
LmnI GCTCC 3 cut(s) 397, 593, 820
Lsp1109I GCAGC 4 cut(s) 57, 425, 598, 1053
LweI GCATC 1 cut(s) 1069
MaeI CTAG 2 cut(s) 47, 944
MaeII ACGT 3 cut(s) 54, 770, 933
MaeIII GTNAC 1 cut(s) 491
MalI GATC 4 cut(s) 304, 399, 522, 537
MboI GATC 4 cut(s) 302, 397, 520, 535
MboII GAAGA 7 cut(s) 170, 223, 325, 372, 396, 935, 1012
MhlI GDGCHC 2 cut(s) 558, 861
MluCI AATT 3 cut(s) 368, 647, 840
MlyI GAGTC 2 cut(s) 691, 1082
MmeI TCCRAC 3 cut(s) 873, 1062, 1078
Mph1103I ATGCAT 1 cut(s) 533
MslI CAYNNNNRTG 2 cut(s) 451, 1082
MspA1I CMGCKG 1 cut(s) 1066
MspI CCGG 4 cut(s) 978, 1011, 1046, 1070
MspR9I CCNGG 4 cut(s) 325, 462, 1012, 1046
MvaI CCWGG 2 cut(s) 325, 462
MvnI CGCG 3 cut(s) 68, 83, 223
MwoI GCNNNNNNNGC 3 cut(s) 56, 65, 1066
NciI CCSGG 2 cut(s) 1012, 1046
NcoI CCATGG 2 cut(s) 417, 1083
NdeII GATC 4 cut(s) 302, 397, 520, 535
NheI GCTAGC 1 cut(s) 943
NlaIII CATG 6 cut(s) 10, 421, 531, 832, 923, 1087
NlaIV GGNNCC 2 cut(s) 458, 859
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 1 cut(s) 531
OliI CACNNNNGTG 1 cut(s) 451
PaeI GCATGC 1 cut(s) 531
PasI CCCWGGG 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfeI GAWTC 4 cut(s) 311, 465, 689, 788
PkrI GCNGC 5 cut(s) 72, 225, 415, 613, 1068
PleI GAGTC 2 cut(s) 691, 1082
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
PpsI GAGTC 2 cut(s) 691, 1082
Ppu21I YACGTR 2 cut(s) 55, 934
PshAI GACNNNNGTC 1 cut(s) 701
Psp6I CCWGG 2 cut(s) 323, 460
PspCI CACGTG 1 cut(s) 55
PspFI CCCAGC 2 cut(s) 144, 603
PspGI CCWGG 2 cut(s) 323, 460
PspN4I GGNNCC 2 cut(s) 458, 859
PspOMI GGGCCC 1 cut(s) 857
PspPI GGNCC 6 cut(s) 632, 809, 857, 858, 1013, 1048
PsrI GAACNNNNNNTAC 2 cut(s) 785, 817
RsaI GTAC 3 cut(s) 547, 584, 662
RsaNI GTAC 3 cut(s) 546, 583, 661
RseI CAYNNNNRTG 2 cut(s) 451, 1082
SalI GTCGAC 1 cut(s) 704
SatI GCNGC 5 cut(s) 71, 224, 414, 612, 1067
Sau3AI GATC 4 cut(s) 302, 397, 520, 535
Sau96I GGNCC 6 cut(s) 632, 809, 857, 858, 1013, 1048
ScaI AGTACT 1 cut(s) 662
SchI GAGTC 2 cut(s) 691, 1082
ScrFI CCNGG 4 cut(s) 325, 462, 1012, 1046
SduI GDGCHC 2 cut(s) 558, 861
SfaNI GCATC 1 cut(s) 1069
SfcI CTRYAG 1 cut(s) 505
SinI GGWCC 1 cut(s) 809
SmiMI CAYNNNNRTG 2 cut(s) 451, 1082
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
SphI GCATGC 1 cut(s) 531
Sse9I AATT 3 cut(s) 368, 647, 840
SsiI CCGC 5 cut(s) 83, 223, 319, 864, 1064
SspMI CTAG 2 cut(s) 47, 944
StyD4I CCNGG 4 cut(s) 323, 460, 1010, 1044
StyI CCWWGG 3 cut(s) 417, 805, 1083
TaaI ACNGT 2 cut(s) 116, 280
TaiI ACGT 3 cut(s) 57, 773, 936
TaqI TCGA 7 cut(s) 101, 282, 301, 309, 468, 705, 791
TasI AATT 3 cut(s) 368, 647, 840
TatI WGTACW 1 cut(s) 660
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 4 cut(s) 311, 465, 689, 788
TscAI CASTG 2 cut(s) 42, 273
TseI GCWGC 4 cut(s) 70, 413, 611, 1066
TspDTI ATGAA 3 cut(s) 225, 334, 936
TspRI CASTG 2 cut(s) 42, 273
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 531
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 2 cut(s) 47, 944
ZrmI AGTACT 1 cut(s) 662
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.