Rh6CG465800

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010804
Physical Location & Seq
Forward (+)
63800799 .. 63803459
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG465800.1

Sequence Viewer

Length: 420 bp
ATGGTATTCTGGGTGTTTGGATATGGTTCACTTGTGTGGAACCCTGGTTTCGAATACGACGAGAAAGTGATAGGTTTCATCAAGGACTACAGGCGTGTATTCGATCTTGCTTGCATTGATCATCGAGGTACGCCTGAGCACCCTGCAAGAACTTGCACATTGGAACACCAAGAAGGGGCTATTTGCTGGGGTGCTGCTTATTCTGTGCGTGGCGGCCCTGAAAGGGAAAGACTGGCTATGGAGCCCATGAAGATAATTGTGATAGAGCTTGCAAATGAGGTGAGGAATGTACTTGGAATTGTTGGGAAAGGAATTCCAAAGGATAAGAAGTTGACAGGACCAACCCACATCCCACTCAAGTCCCACATTCCAGCCATTCAATTGCATCCGCTTCCTGAAGCCATCGCCATGGACTCGTAG

Protein Analysis

139

Amino Acids

15.57

Weight (kDa)

6.58

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 74 4.9e-23 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 213, 389
AcsI RAATTY 1 cut(s) 312
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 2 cut(s) 130, 291
AfiI CCNNNNNNNGG 2 cut(s) 175, 223
AgsI TTSAA 1 cut(s) 380
AjnI CCWGG 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 34
AluBI AGCT 1 cut(s) 268
AluI AGCT 1 cut(s) 268
Alw21I GWGCWC 1 cut(s) 141
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 215, 338
AsuHPI GGTGA 1 cut(s) 292
AsuII TTCGAA 1 cut(s) 51
AvaII GGWCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 410
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 118
BfmI CTRYAG 1 cut(s) 88
BisI GCNGC 2 cut(s) 195, 214
BlsI GCNGC 2 cut(s) 196, 215
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 215, 338
BmiI GGNNCC 2 cut(s) 41, 243
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 135
Bpu14I TTCGAA 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 341
BsaJI CCNNGG 2 cut(s) 43, 408
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 223
Bse1I ACTGG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 43, 408
BseGI GGATG 2 cut(s) 348, 385
BseLI CCNNNNNNNGG 2 cut(s) 175, 223
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 186
BshFI GGCC 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 141
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 2 cut(s) 175, 223
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 141, 246
Bsp143I GATC 2 cut(s) 103, 118
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 2 cut(s) 213, 389
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 41, 243
BspT104I TTCGAA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 43, 408
BssMI GATC 2 cut(s) 103, 118
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 112, 270
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 348, 385
BstKTI GATC 2 cut(s) 106, 121
BstMBI GATC 2 cut(s) 103, 118
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuRI GGCC 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 388
BtsCI GGATG 2 cut(s) 348, 385
Cac8I GCNNGC 2 cut(s) 112, 270
Cfr13I GGNCC 2 cut(s) 215, 338
Csp6I GTAC 2 cut(s) 129, 290
CviAII CATG 2 cut(s) 247, 409
CviJI RGCY 7 cut(s) 179, 216, 236, 244, 268, 374, 401
CviKI_1 RGCY 7 cut(s) 179, 216, 236, 244, 268, 374, 401
CviQI GTAC 2 cut(s) 129, 290
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 105, 120
DpnII GATC 2 cut(s) 103, 118
Eco130I CCWWGG 1 cut(s) 408
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 417
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 2 cut(s) 250, 412
FaiI YATR 4 cut(s) 24, 239, 248, 410
FaqI GGGAC 1 cut(s) 346
FatI CATG 2 cut(s) 246, 408
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 195, 214
FokI GGATG 2 cut(s) 335, 372
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 2 cut(s) 195, 214
GluI GCNGC 2 cut(s) 195, 214
GsaI CCCAGC 1 cut(s) 190
HaeIII GGCC 1 cut(s) 216
Hin1II CATG 2 cut(s) 250, 412
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 1 cut(s) 413
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 29, 333
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 2 cut(s) 29, 333
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 167
HpyCH4V TGCA 5 cut(s) 114, 146, 156, 272, 385
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 2 cut(s) 250, 412
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 103, 118
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 394
MalI GATC 2 cut(s) 105, 120
MboI GATC 2 cut(s) 103, 118
MboII GAAGA 1 cut(s) 262
MfeI CAATTG 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 141, 246
MluCI AATT 4 cut(s) 255, 297, 312, 380
MlyI GAGTC 1 cut(s) 407
MnlI CCTC 3 cut(s) 119, 271, 276
MslI CAYNNNNRTG 2 cut(s) 34, 407
MspR9I CCNGG 1 cut(s) 45
MunI CAATTG 1 cut(s) 380
MvaI CCWGG 1 cut(s) 45
NcoI CCATGG 1 cut(s) 408
NdeII GATC 2 cut(s) 103, 118
NlaIII CATG 2 cut(s) 250, 412
NlaIV GGNNCC 2 cut(s) 41, 243
NspV TTCGAA 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PkrI GCNGC 2 cut(s) 196, 215
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 41, 243
PspPI GGNCC 2 cut(s) 215, 338
RsaI GTAC 2 cut(s) 130, 291
RsaNI GTAC 2 cut(s) 129, 290
RseI CAYNNNNRTG 2 cut(s) 34, 407
SatI GCNGC 2 cut(s) 195, 214
Sau3AI GATC 2 cut(s) 103, 118
Sau96I GGNCC 2 cut(s) 215, 338
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 2 cut(s) 141, 246
SetI ASST 4 cut(s) 76, 130, 270, 282
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 2 cut(s) 34, 407
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 4 cut(s) 255, 297, 312, 380
SsiI CCGC 2 cut(s) 213, 389
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 408
TaqI TCGA 3 cut(s) 51, 102, 124
TasI AATT 4 cut(s) 255, 297, 312, 380
TatI WGTACW 1 cut(s) 289
TauI GCSGC 1 cut(s) 216
TseI GCWGC 1 cut(s) 194
TspDTI ATGAA 2 cut(s) 67, 263
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.