FvH4_2g36490

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010804
Physical Location & Seq
Reverse (-)
26703327 .. 26705917
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36490.t1

Sequence Viewer

Length: 675 bp
ATGGTGTTCTGGGTTTTTGGATATGGTTCACTTGTGTGGAACCCTGGTTTCGAGTACGACGAGAAAGTGATAGGTTTCATCAAGGACTACAAGCGTGTTTTCGATCTTGCTTGCATTGATCATCGGGGTACACCTGAGCACCCTGCAAGAACTTGCACATTGGAACACCAAGAAGGGGCTATTTGCTGGGGTGCTGCTTACTCTGTGCGTGGCGGCCCTGAAAAGGAAAGACTTGCTATGGAGTACTTGGAAAAGAGAGAATGCGAATATGATGCAAAGACTCTTGTGAACTTTTACAAGGAAGGGGACTTCTCTGATGAGCCTTCTTTGACAGGAGTCATTGTTTTCACATCTACTCCAGACAAAGAATCAAACAAGTATTACTTGGGTCCTGCTCCACTGGAGGATATGGCAAGACAAATTGCAACTGCTTCTGGGCCATGTGGAAACAACAGAGAGTACTTATTTCTGCTGGAAAAGGCTATGTTTGATATTGGCCATGAAGAGGATTATATTATAGAGCTTGCAAATGAGGTGAGGAATGTACTTGGAATTGTTGGGAAGGGAATTCCAAAGGATAAGAAGTTGACTGGACCTACCCACATCCCACTCAAGTCCCACATCCCAGTCATTCACTTGCATCCTCTTCCTGAAGCCATTGCCATGGACTCATAG

Protein Analysis

225

Amino Acids

25.26

Weight (kDa)

5.2

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 181 1.9e-45 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 213
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 1 cut(s) 567
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 5 cut(s) 56, 130, 245, 461, 546
AfiI CCNNNNNNNGG 3 cut(s) 175, 223, 505
AjnI CCWGG 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 34
AluBI AGCT 1 cut(s) 523
AluI AGCT 1 cut(s) 523
Alw21I GWGCWC 1 cut(s) 141
AoxI GGCC 3 cut(s) 214, 437, 496
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 567
AspS9I GGNCC 4 cut(s) 215, 389, 437, 593
AsuHPI GGTGA 1 cut(s) 547
AvaII GGWCC 2 cut(s) 389, 593
BalI TGGCCA 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 181
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 118
BisI GCNGC 2 cut(s) 195, 214
BlsI GCNGC 2 cut(s) 196, 215
BmcAI AGTACT 2 cut(s) 245, 461
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 2 cut(s) 389, 593
BmgT120I GGNCC 4 cut(s) 215, 389, 437, 593
BmiI GGNNCC 2 cut(s) 41, 390
BmrFI CCNGG 1 cut(s) 45
BmrI ACTGGG 1 cut(s) 620
BmsI GCATC 2 cut(s) 262, 649
BmuI ACTGGG 1 cut(s) 620
BoxI GACNNNNGTC 1 cut(s) 335
BpmI CTGGAG 2 cut(s) 342, 422
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 43, 663
BsaXI ACNNNNNCTCC 4 cut(s) 327, 340, 357, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 223, 505
Bse1I ACTGG 3 cut(s) 405, 595, 626
Bse3DI GCAATG 1 cut(s) 657
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 43, 663
BseGI GGATG 3 cut(s) 603, 621, 640
BseLI CCNNNNNNNGG 3 cut(s) 175, 223, 505
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 3 cut(s) 405, 595, 626
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 186
BshFI GGCC 3 cut(s) 216, 439, 498
BsiHKAI GWGCWC 1 cut(s) 141
BslFI GGGAC 2 cut(s) 320, 601
BslI CCNNNNNNNGG 3 cut(s) 175, 223, 505
BsmFI GGGAC 2 cut(s) 320, 601
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 3 cut(s) 216, 439, 498
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 103, 118
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 1 cut(s) 213
BspANI GGCC 3 cut(s) 216, 439, 498
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 41, 390
BsrDI GCAATG 1 cut(s) 657
BsrI ACTGG 3 cut(s) 405, 595, 626
BssECI CCNNGG 2 cut(s) 43, 663
BssMI GATC 2 cut(s) 103, 118
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 45
Bst6I CTCTTC 2 cut(s) 498, 651
BstC8I GCNNGC 2 cut(s) 112, 525
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 3 cut(s) 603, 621, 640
BstKTI GATC 2 cut(s) 106, 121
BstMBI GATC 2 cut(s) 103, 118
BstNI CCWGG 1 cut(s) 45
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 664
BsuRI GGCC 3 cut(s) 216, 439, 498
BtgI CCRYGG 1 cut(s) 663
BtsCI GGATG 3 cut(s) 603, 621, 640
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 2 cut(s) 112, 525
Cfr13I GGNCC 4 cut(s) 215, 389, 437, 593
Csp6I GTAC 5 cut(s) 55, 129, 244, 460, 545
CviAII CATG 3 cut(s) 441, 500, 664
CviJI RGCY 8 cut(s) 179, 216, 322, 439, 482, 498, 523, 656
CviKI_1 RGCY 8 cut(s) 179, 216, 322, 439, 482, 498, 523, 656
CviQI GTAC 5 cut(s) 55, 129, 244, 460, 545
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 105, 120
DpnII GATC 2 cut(s) 103, 118
EaeI YGGCCR 1 cut(s) 496
Eam1104I CTCTTC 2 cut(s) 498, 651
EarI CTCTTC 2 cut(s) 498, 651
Eco130I CCWWGG 1 cut(s) 663
Eco47I GGWCC 2 cut(s) 389, 593
Eco57I CTGAAG 1 cut(s) 672
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 3 cut(s) 444, 503, 667
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 2 cut(s) 320, 601
FatI CATG 3 cut(s) 440, 499, 663
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 195, 214
FokI GGATG 3 cut(s) 590, 608, 627
Fsp4HI GCNGC 2 cut(s) 195, 214
GluI GCNGC 2 cut(s) 195, 214
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 2 cut(s) 342, 422
HaeIII GGCC 3 cut(s) 216, 439, 498
Hin1II CATG 3 cut(s) 444, 503, 667
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HinfI GANTC 4 cut(s) 280, 336, 368, 668
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 4 cut(s) 29, 131, 289, 588
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 359, 650
Hpy8I GTNNAC 4 cut(s) 29, 131, 289, 588
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 4 cut(s) 167, 296, 333, 556
HpyCH4V TGCA 7 cut(s) 114, 146, 156, 275, 425, 527, 640
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 3 cut(s) 444, 503, 667
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 103, 118
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 2 cut(s) 262, 649
MalI GATC 2 cut(s) 105, 120
MboI GATC 2 cut(s) 103, 118
MboII GAAGA 2 cut(s) 515, 638
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 3 cut(s) 420, 552, 567
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 3 cut(s) 274, 345, 662
MnlI CCTC 5 cut(s) 397, 499, 526, 531, 654
Mox20I TGGCCA 1 cut(s) 498
MscI TGGCCA 1 cut(s) 498
MslI CAYNNNNRTG 2 cut(s) 34, 662
Msp20I TGGCCA 1 cut(s) 498
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 1 cut(s) 45
NcoI CCATGG 1 cut(s) 663
NdeII GATC 2 cut(s) 103, 118
NlaIII CATG 3 cut(s) 444, 503, 667
NlaIV GGNNCC 2 cut(s) 41, 390
OliI CACNNNNGTG 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 2 cut(s) 196, 215
PleI GAGTC 3 cut(s) 274, 344, 662
PpsI GAGTC 3 cut(s) 274, 344, 662
PpuMI RGGWCCY 1 cut(s) 389
PshAI GACNNNNGTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 41, 390
PspPI GGNCC 4 cut(s) 215, 389, 437, 593
PspPPI RGGWCCY 1 cut(s) 389
RsaI GTAC 5 cut(s) 56, 130, 245, 461, 546
RsaNI GTAC 5 cut(s) 55, 129, 244, 460, 545
RseI CAYNNNNRTG 2 cut(s) 34, 662
SatI GCNGC 2 cut(s) 195, 214
Sau3AI GATC 2 cut(s) 103, 118
Sau96I GGNCC 4 cut(s) 215, 389, 437, 593
ScaI AGTACT 2 cut(s) 245, 461
SchI GAGTC 3 cut(s) 274, 345, 662
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 1 cut(s) 141
SetI ASST 5 cut(s) 76, 136, 525, 537, 598
SfaNI GCATC 2 cut(s) 262, 649
SinI GGWCC 2 cut(s) 389, 593
SmiMI CAYNNNNRTG 2 cut(s) 34, 662
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 3 cut(s) 420, 552, 567
SsiI CCGC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 663
TaqI TCGA 2 cut(s) 51, 102
TasI AATT 3 cut(s) 420, 552, 567
TatI WGTACW 3 cut(s) 243, 459, 544
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 368
TscAI CASTG 1 cut(s) 405
TseI GCWGC 1 cut(s) 194
TspDTI ATGAA 2 cut(s) 67, 516
TspRI CASTG 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 389, 593
XapI RAATTY 1 cut(s) 567
ZrmI AGTACT 2 cut(s) 245, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.