AT4G32440

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003723
Physical Location & Seq
Forward (+)
15656681 .. 15660223
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32440.1

Sequence Viewer

Length: 1134 bp
ATGAGAATCAGAAAAGGAAGTAGAGTTGAGGTATTTAGCAACAAAGAGGCGCCTTATGGTGCGTGGCGATGTGCTGAGATTGTCTCGGGTAATGGACATACCTACAATGTTAGATTTTACTCTTTCCAAATTGAACACGAGGAGGCAGTTATGGAGAAAGTTCCAAGGAAGATAATTAGGCCTTGTCCTCCACTTGTGGATGTTGAGAGATGGGATACTGGTGAATTGGTGGAAGTTCTTGATAATTTTTCCTGGAAAGCTGCCACAGTTCGAGAGGAGTTATCTGGACATTACTATGTGGTTCGCTTACTTGGGACTCCAGAAGAACTCACATTTCACAAAGTTAACCTCAGGGCCCGAAAGTCGTGGCAAGATGAGAGATGGGTTGCAATTGGAAAGATATCTGGTTCTCTGAAGTCATCCACACTGACTGGATCAGACGTACATCAGAAGCTACAGCCCCACAGGAACAGCATGCCTCTCCACGAGCCTAGCGTGGTCTCTGCTAGATTGTTAAAGAGGCCGTCACCTTACAACTGGTCTGAATGTGCTGAATCATGTACAGGAAACCCTAAGAAGATGCGTTCATTGGAAAAAGAAGGACAGCAGCAAAAGGTAGATGCCATTTCTTGCCGACCAGAAAACAGGGGTGGTAAATCTCACGTGCAAGCTTCCTTAAACAATCACAAAACTGGTTACTGTCAAATTGTCAGGGTAAGATCAAAAGGGTTTAGTGAGAGTGTTCGTGCTGATGATTGTTCTGATAGCGATGTATGCTCAGTTGGTAGTTGTAGTGCTACTAGTTATGATGAGAGTAACATGCCACCTTGTATGCTAGATGGCTCTACTCAACAGGCAGACTCATGTAGCAGCGATGCTGAATCTTCTTGTGGCCTGGGGGAAGAACCAAGGTGGAAACATTCATCAGTTGGTGATGGAGCAAGAAATTCTTGTAGGTCGGAACTATATTCTTACCGCAGTACTCTGGGGGAATTATTTTCTTCTGGTCCCCTAAGTTGGGAGCAAGAAGCATCATTAACTGATCTTCGTCTTTCTCTTAATATATCAGATGATGAACATTTGATGGAGGTAAGAAATTTGATATCTACTGGTACCCGCAGTCAATTTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

377

Amino Acids

42.22

Weight (kDa)

6.61

Isoelectric Point (pI)

53.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 6 - 64 5e-16 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1112
AccB1I GGYRCC 2 cut(s) 49, 1112
AciI CCGC 2 cut(s) 976, 1117
AclWI GGATC 1 cut(s) 442
AcsI RAATTY 2 cut(s) 946, 1096
AcuI CTGAAG 1 cut(s) 434
AcvI CACGTG 1 cut(s) 664
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 4 cut(s) 444, 562, 982, 1114
AfiI CCNNNNNNNGG 2 cut(s) 1017, 1018
AgsI TTSAA 1 cut(s) 134
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 2 cut(s) 251, 894
AluBI AGCT 3 cut(s) 260, 454, 671
AluI AGCT 3 cut(s) 260, 454, 671
Alw26I GTCTC 2 cut(s) 88, 505
AlwI GGATC 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 4 cut(s) 179, 354, 521, 892
ApaI GGGCCC 1 cut(s) 358
ApeKI GCWGC 3 cut(s) 260, 607, 870
ApoI RAATTY 2 cut(s) 946, 1096
Asp718I GGTACC 1 cut(s) 1112
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 3 cut(s) 354, 355, 1007
AsuHPI GGTGA 3 cut(s) 233, 519, 944
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 1007
AxyI CCTNAGG 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 358
BanI GGYRCC 2 cut(s) 49, 1112
BanII GRGCYC 1 cut(s) 358
BauI CACGAG 2 cut(s) 137, 485
BbrPI CACGTG 1 cut(s) 664
BbvI GCAGC 3 cut(s) 247, 619, 882
BccI CCATC 5 cut(s) 204, 375, 833, 929, 1078
BceAI ACGGC 1 cut(s) 508
BciT130I CCWGG 2 cut(s) 253, 896
BciVI GTATCC 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 88, 505
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 4 cut(s) 492, 507, 801, 836
BfmI CTRYAG 1 cut(s) 455
BfoI RGCGCY 1 cut(s) 53
BfuI GTATCC 1 cut(s) 208
BisI GCNGC 3 cut(s) 261, 608, 871
BlsI GCNGC 3 cut(s) 262, 609, 872
BmcAI AGTACT 1 cut(s) 982
Bme1390I CCNGG 2 cut(s) 253, 896
Bme18I GGWCC 1 cut(s) 1007
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 3 cut(s) 354, 355, 1007
BmiI GGNNCC 4 cut(s) 51, 356, 1009, 1114
BmrFI CCNGG 2 cut(s) 253, 896
BmsI GCATC 4 cut(s) 570, 610, 865, 1040
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BpmI CTGGAG 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 50
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 164, 895, 908
Bsc4I CCNNNNNNNGG 2 cut(s) 1017, 1018
Bse1I ACTGG 5 cut(s) 223, 436, 542, 697, 1114
Bse21I CCTNAGG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 253, 896
BseDI CCNNGG 3 cut(s) 164, 895, 908
BseGI GGATG 2 cut(s) 205, 419
BseLI CCNNNNNNNGG 2 cut(s) 1017, 1018
BseMII CTCAG 3 cut(s) 66, 364, 792
BseNI ACTGG 5 cut(s) 223, 436, 542, 697, 1114
BseRI GAGGAG 2 cut(s) 155, 290
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 3 cut(s) 247, 619, 882
BshFI GGCC 4 cut(s) 181, 356, 523, 894
BshNI GGYRCC 2 cut(s) 49, 1112
BsiHKCI CYCGRG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 328, 993
BslI CCNNNNNNNGG 2 cut(s) 1017, 1018
BsmAI GTCTC 2 cut(s) 88, 505
BsmFI GGGAC 2 cut(s) 328, 993
BsnI GGCC 4 cut(s) 181, 356, 523, 894
Bso31I GGTCTC 1 cut(s) 505
BsoBI CYCGRG 1 cut(s) 85
Bsp120I GGGCCC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 358
Bsp1407I TGTACA 1 cut(s) 560
Bsp143I GATC 3 cut(s) 434, 719, 1042
BspACI CCGC 2 cut(s) 976, 1117
BspANI GGCC 4 cut(s) 181, 356, 523, 894
BspCNI CTCAG 3 cut(s) 67, 363, 791
BspLI GGNNCC 4 cut(s) 51, 356, 1009, 1114
BspPI GGATC 1 cut(s) 442
BspT107I GGYRCC 2 cut(s) 49, 1112
BspTNI GGTCTC 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 560
BsrI ACTGG 5 cut(s) 223, 436, 542, 697, 1114
BssECI CCNNGG 3 cut(s) 164, 895, 908
BssMI GATC 3 cut(s) 434, 719, 1042
BssNI GRCGYC 1 cut(s) 50
BssSI CACGAG 2 cut(s) 137, 485
BssT1I CCWWGG 2 cut(s) 164, 908
Bst2BI CACGAG 2 cut(s) 137, 485
Bst2UI CCWGG 2 cut(s) 253, 896
Bst4CI ACNGT 2 cut(s) 268, 701
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 476, 669
BstDEI CTNAG 5 cut(s) 75, 350, 573, 778, 1013
BstF5I GGATG 2 cut(s) 205, 419
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 3 cut(s) 437, 722, 1045
BstMAI GTCTC 2 cut(s) 88, 505
BstMBI GATC 3 cut(s) 434, 719, 1042
BstMWI GCNNNNNNNGC 1 cut(s) 774
BstNI CCWGG 2 cut(s) 253, 896
BstNSI RCATGY 2 cut(s) 478, 823
BstSCI CCNGG 2 cut(s) 251, 894
BstSFI CTRYAG 1 cut(s) 455
BstSLI GKGCMC 1 cut(s) 358
BstV1I GCAGC 3 cut(s) 247, 619, 882
Bsu36I CCTNAGG 1 cut(s) 350
BsuI GTATCC 1 cut(s) 208
BsuRI GGCC 4 cut(s) 181, 356, 523, 894
BtgZI GCGATG 3 cut(s) 82, 783, 888
BtsCI GGATG 2 cut(s) 205, 419
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 2 cut(s) 476, 669
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 3 cut(s) 354, 355, 1007
Csp6I GTAC 4 cut(s) 443, 561, 981, 1113
CviAII CATG 4 cut(s) 475, 558, 820, 864
CviQI GTAC 4 cut(s) 443, 561, 981, 1113
DdeI CTNAG 5 cut(s) 75, 350, 573, 778, 1013
DinI GGCGCC 1 cut(s) 51
DpnI GATC 3 cut(s) 436, 721, 1044
DpnII GATC 3 cut(s) 434, 719, 1042
Eco130I CCWWGG 2 cut(s) 164, 908
Eco147I AGGCCT 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 505
Eco32I GATATC 2 cut(s) 402, 1104
Eco47I GGWCC 1 cut(s) 1007
Eco57I CTGAAG 1 cut(s) 434
Eco72I CACGTG 1 cut(s) 664
Eco81I CCTNAGG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 354
EcoRII CCWGG 2 cut(s) 251, 894
EcoRV GATATC 2 cut(s) 402, 1104
EcoT14I CCWWGG 2 cut(s) 164, 908
EcoT38I GRGCYC 1 cut(s) 358
EgeI GGCGCC 1 cut(s) 51
EheI GGCGCC 1 cut(s) 51
ErhI CCWWGG 2 cut(s) 164, 908
FaeI CATG 4 cut(s) 478, 561, 823, 867
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FaqI GGGAC 2 cut(s) 328, 993
FatI CATG 4 cut(s) 474, 557, 819, 863
FauI CCCGC 1 cut(s) 1124
Fnu4HI GCNGC 3 cut(s) 261, 608, 871
FokI GGATG 2 cut(s) 212, 406
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 3 cut(s) 261, 608, 871
FspBI CTAG 4 cut(s) 492, 507, 801, 836
GlaI GCGC 1 cut(s) 51
GluI GCNGC 3 cut(s) 261, 608, 871
GsuI CTGGAG 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 4 cut(s) 181, 356, 523, 894
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 4 cut(s) 478, 561, 823, 867
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 669
HinfI GANTC 5 cut(s) 6, 316, 554, 860, 881
HpaI GTTAAC 1 cut(s) 346
HphI GGTGA 3 cut(s) 233, 519, 944
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 8 cut(s) 11, 414, 439, 450, 544, 763, 961, 1069
Hpy188III TCNNGA 4 cut(s) 239, 272, 285, 320
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 1 cut(s) 593
HpyCH4III ACNGT 2 cut(s) 268, 701
HpyCH4IV ACGT 2 cut(s) 441, 663
HpyCH4V TGCA 2 cut(s) 389, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 774
HpyF3I CTNAG 5 cut(s) 75, 350, 573, 778, 1013
HpySE526I ACGT 2 cut(s) 441, 663
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 4 cut(s) 478, 561, 823, 867
HspAI GCGC 1 cut(s) 50
KasI GGCGCC 1 cut(s) 49
KpnI GGTACC 1 cut(s) 1116
KspAI GTTAAC 1 cut(s) 346
Kzo9I GATC 3 cut(s) 434, 719, 1042
LmnI GCTCC 2 cut(s) 938, 1021
Lsp1109I GCAGC 3 cut(s) 247, 619, 882
LweI GCATC 4 cut(s) 570, 610, 865, 1040
MaeI CTAG 4 cut(s) 492, 507, 801, 836
MaeII ACGT 2 cut(s) 441, 663
MaeIII GTNAC 3 cut(s) 525, 695, 815
MalI GATC 3 cut(s) 436, 721, 1044
MboI GATC 3 cut(s) 434, 719, 1042
MboII GAAGA 7 cut(s) 181, 335, 589, 876, 914, 993, 1037
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 1 cut(s) 358
Mly113I GGCGCC 1 cut(s) 50
MlyI GAGTC 2 cut(s) 310, 854
MmeI TCCRAC 1 cut(s) 939
MseI TTAA 5 cut(s) 345, 515, 677, 1037, 1059
MslI CAYNNNNRTG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 253, 896
MunI CAATTG 1 cut(s) 390
MvaI CCWGG 2 cut(s) 253, 896
MwoI GCNNNNNNNGC 1 cut(s) 774
NarI GGCGCC 1 cut(s) 50
NdeII GATC 3 cut(s) 434, 719, 1042
NlaIII CATG 4 cut(s) 478, 561, 823, 867
NlaIV GGNNCC 4 cut(s) 51, 356, 1009, 1114
NmuCI GTSAC 1 cut(s) 525
NspI RCATGY 2 cut(s) 478, 823
PaeI GCATGC 1 cut(s) 478
PceI AGGCCT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PfeI GAWTC 3 cut(s) 6, 554, 881
PfoI TCCNGGA 1 cut(s) 251
PkrI GCNGC 3 cut(s) 262, 609, 872
PleI GAGTC 2 cut(s) 310, 854
PluTI GGCGCC 1 cut(s) 53
PmaCI CACGTG 1 cut(s) 664
PmlI CACGTG 1 cut(s) 664
PpsI GAGTC 2 cut(s) 310, 854
Ppu21I YACGTR 1 cut(s) 664
Psp6I CCWGG 2 cut(s) 251, 894
PspCI CACGTG 1 cut(s) 664
PspGI CCWGG 2 cut(s) 251, 894
PspN4I GGNNCC 4 cut(s) 51, 356, 1009, 1114
PspOMI GGGCCC 1 cut(s) 354
PspPI GGNCC 3 cut(s) 354, 355, 1007
RsaI GTAC 4 cut(s) 444, 562, 982, 1114
RsaNI GTAC 4 cut(s) 443, 561, 981, 1113
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 5 cut(s) 345, 515, 677, 1037, 1059
SatI GCNGC 3 cut(s) 261, 608, 871
Sau3AI GATC 3 cut(s) 434, 719, 1042
Sau96I GGNCC 3 cut(s) 354, 355, 1007
ScaI AGTACT 1 cut(s) 982
SchI GAGTC 2 cut(s) 310, 854
ScrFI CCNGG 2 cut(s) 253, 896
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 4 cut(s) 570, 610, 865, 1040
SfcI CTRYAG 1 cut(s) 455
SfoI GGCGCC 1 cut(s) 51
SinI GGWCC 1 cut(s) 1007
SmiMI CAYNNNNRTG 1 cut(s) 294
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 478
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 2 cut(s) 976, 1117
SspDI GGCGCC 1 cut(s) 49
SspMI CTAG 4 cut(s) 492, 507, 801, 836
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 251, 894
StyI CCWWGG 2 cut(s) 164, 908
TaaI ACNGT 2 cut(s) 268, 701
TaiI ACGT 2 cut(s) 444, 666
TaqI TCGA 1 cut(s) 271
TatI WGTACW 2 cut(s) 560, 980
TfiI GAWTC 3 cut(s) 6, 554, 881
Tru1I TTAA 5 cut(s) 345, 515, 677, 1037, 1059
Tru9I TTAA 5 cut(s) 345, 515, 677, 1037, 1059
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 3 cut(s) 260, 607, 870
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 3 cut(s) 576, 912, 1089
TspRI CASTG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 1007
XapI RAATTY 2 cut(s) 946, 1096
XceI RCATGY 2 cut(s) 478, 823
XspI CTAG 4 cut(s) 492, 507, 801, 836
ZrmI AGTACT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.