Rw7G032050

ENT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003723
Physical Location & Seq
Forward (+)
46308688 .. 46310724
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G032050.1

Sequence Viewer

Length: 1206 bp
ATGAGATTCAAGAAAGGGAGCAAGGTGGAAGTACTAAACGAAAAGGAGGTGCCTTCAGGAGCATGGCAGTGCGCTGTGATTATATCAGGCAATGGACACACTTACAGTGTTAGGTATGATTGGTCTCCTAACTGGGAAGGTCAAGCAGTTGTTGAGAGAGTCCCGCGGAAGGCTATTAGGCCTTGTCCTCCGCCTGTTCAGGGTGCAGATTGTTGGGCAGTCGGTGATGTTGTTGAGGTCTTTGATGTTGGTTTCTGGAAGATGGCTAACTTAGTGAAGGTTTTTGATGAAAACTATTACTTGGCTAGGCTACTTGGATCATTTGAGGAGTTTAGAATCCACAAATCTAGGATCAGGGTGCGTCAAGTTTTGAAAGATGAGGAATGGATTGTGATTGGAATGGGTCTGGGATCAGCTTACCACAATCATACGAGTTCTCCAGTCTCACGAATGAATTTAAAAATAAAGCTGCCCAATGGAAATGATTGTTTACCTCCTCCGGAAAACAACAGTTGTGAGGATTCCCATATTGTCTCCTCGGCATCATTAAAGAGACCTTCTCAATGTTGCTCTCTTTATACCGAAGCATATCCCCGAAAGATAAGAGCTATTGAGAAAGAATCCGGGCGGCAACAAGTTTTCTCTAGGTCCCCATCTTCCTTGCTTGAGAAGGTAGATGCTGTTGCTTACCCAAGAGAAAATCTGGCTGAAAAATACGTGCACGCTTCCTTTATTTGCCCTTCCACTCAATATTGTGAAAATGAGAAGCGAATTTTAAATGGTGCCAATTCATGTTTTGAAAGAAGCTCGGATGGTGTTTTTGATAGTGATGCATGCTCTATTGAATCTGTTGGTAGTTGTAGTGTTATTAGTAACAGTTCAAATAAGTTGTCCGGGTATAATCTAGCCGGTCACAGTGAAGATGAAGATACCCTTTGTAGTGATGCAGACTCTTTTTCTGGTGGCGCTGATGTGCAAGAATTTAATTCCTTTCCTCTGAAAGGGGATGTAACAGCAAGAGACTCTAGGTCGGAGTTGCATGCTTATCGCCGTACTCTGATGTTAATGCATGCTTCAGGTTCTTTAAGTTGGGAACAAGAAGATTTATTGACAAACCTCCGTATGTCCTTACACATTTCCAATGATGAACATTTGATGGAAGGCCATGGTTGGGAGGCTTGGCATGCCTTGCTTCCAAAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

401

Amino Acids

44.91

Weight (kDa)

5.85

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 6 - 64 1.3e-16 Agenet domain
ENT PF03735 345 - 385 1.3e-07 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 49, 782
AccII CGCG 1 cut(s) 166
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 4 cut(s) 164, 166, 191, 628
AclWI GGATC 3 cut(s) 325, 359, 418
AcsI RAATTY 3 cut(s) 454, 771, 980
AcuI CTGAAG 2 cut(s) 39, 1059
AfaI GTAC 2 cut(s) 33, 1054
AfiI CCNNNNNNNGG 4 cut(s) 169, 200, 1001, 1171
AgsI TTSAA 5 cut(s) 10, 373, 800, 845, 882
AhdI GACNNNNNGTC 1 cut(s) 1027
AluBI AGCT 4 cut(s) 416, 469, 608, 807
AluI AGCT 4 cut(s) 416, 469, 608, 807
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 5 cut(s) 129, 448, 538, 547, 1014
Alw44I GTGCAC 1 cut(s) 719
AlwI GGATC 3 cut(s) 325, 359, 418
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 2 cut(s) 179, 1162
ApaLI GTGCAC 1 cut(s) 719
ApeKI GCWGC 1 cut(s) 469
ApoI RAATTY 3 cut(s) 454, 771, 980
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
AspLEI GCGC 2 cut(s) 74, 968
AspS9I GGNCC 1 cut(s) 648
AsuC2I CCSGG 2 cut(s) 625, 895
AsuHPI GGTGA 1 cut(s) 236
AvaII GGWCC 1 cut(s) 648
BaeGI GKGCMC 1 cut(s) 723
BanI GGYRCC 2 cut(s) 49, 782
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 1 cut(s) 456
BccI CCATC 4 cut(s) 256, 661, 806, 1150
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BcnI CCSGG 2 cut(s) 625, 895
BcoDI GTCTC 5 cut(s) 129, 448, 538, 547, 1014
BfaI CTAG 5 cut(s) 306, 348, 645, 905, 1026
BfoI RGCGCY 1 cut(s) 969
BisI GCNGC 2 cut(s) 470, 629
BlsI GCNGC 2 cut(s) 471, 630
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 2 cut(s) 625, 895
Bme18I GGWCC 1 cut(s) 648
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmgT120I GGNCC 1 cut(s) 648
BmiI GGNNCC 3 cut(s) 51, 650, 784
BmrFI CCNGG 2 cut(s) 625, 895
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 4 cut(s) 551, 667, 820, 934
BmuI ACTGGG 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 544, 576
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 2 cut(s) 625, 895
BsaAI YACGTR 1 cut(s) 718
BsaI GGTCTC 2 cut(s) 129, 547
BsaJI CCNNGG 3 cut(s) 164, 537, 1165
BsaWI WCCGGW 1 cut(s) 499
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 200, 1001, 1171
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 2 cut(s) 137, 440
Bse3DI GCAATG 1 cut(s) 97
BseAI TCCGGA 1 cut(s) 499
BseDI CCNNGG 3 cut(s) 164, 537, 1165
BseGI GGATG 2 cut(s) 817, 1012
BseLI CCNNNNNNNGG 4 cut(s) 169, 200, 1001, 1171
BseMI GCAATG 1 cut(s) 97
BseNI ACTGG 2 cut(s) 137, 440
BseRI GAGGAG 3 cut(s) 341, 486, 526
BseSI GKGCMC 1 cut(s) 723
BseXI GCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 2 cut(s) 181, 1164
BshNI GGYRCC 2 cut(s) 49, 782
BsiHKAI GWGCWC 1 cut(s) 723
BsiSI CCGG 4 cut(s) 500, 624, 894, 909
BslFI GGGAC 2 cut(s) 146, 634
BslI CCNNNNNNNGG 4 cut(s) 169, 200, 1001, 1171
BsmAI GTCTC 5 cut(s) 129, 448, 538, 547, 1014
BsmFI GGGAC 2 cut(s) 146, 634
BsnI GGCC 2 cut(s) 181, 1164
Bso31I GGTCTC 2 cut(s) 129, 547
Bsp1286I GDGCHC 1 cut(s) 723
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 3 cut(s) 317, 351, 410
Bsp19I CCATGG 1 cut(s) 1165
BspACI CCGC 4 cut(s) 164, 166, 191, 628
BspANI GGCC 2 cut(s) 181, 1164
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 1 cut(s) 166
BspLI GGNNCC 3 cut(s) 51, 650, 784
BspPI GGATC 3 cut(s) 325, 359, 418
BspT107I GGYRCC 2 cut(s) 49, 782
BspTNI GGTCTC 2 cut(s) 129, 547
BsrDI GCAATG 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 2 cut(s) 137, 440
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 3 cut(s) 164, 537, 1165
BssMI GATC 3 cut(s) 317, 351, 410
BssT1I CCWWGG 1 cut(s) 1165
Bst4CI ACNGT 4 cut(s) 107, 512, 878, 917
BstAPI GCANNNNNTGC 1 cut(s) 1189
BstBAI YACGTR 1 cut(s) 718
BstC8I GCNNGC 5 cut(s) 723, 835, 1041, 1071, 1185
BstDEI CTNAG 1 cut(s) 271
BstDSI CCRYGG 2 cut(s) 164, 1165
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 2 cut(s) 817, 1012
BstFNI CGCG 1 cut(s) 166
BstH2I RGCGCY 1 cut(s) 969
BstHHI GCGC 2 cut(s) 74, 968
BstKTI GATC 3 cut(s) 320, 354, 413
BstMAI GTCTC 5 cut(s) 129, 448, 538, 547, 1014
BstMBI GATC 3 cut(s) 317, 351, 410
BstMWI GCNNNNNNNGC 2 cut(s) 1184, 1189
BstNSI RCATGY 4 cut(s) 837, 1043, 1073, 1187
BstSCI CCNGG 2 cut(s) 623, 893
BstSLI GKGCMC 1 cut(s) 723
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 456
BsuRI GGCC 2 cut(s) 181, 1164
BtgI CCRYGG 2 cut(s) 164, 1165
BtsCI GGATG 2 cut(s) 817, 1012
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 74, 112, 922
Cac8I GCNNGC 5 cut(s) 723, 835, 1041, 1071, 1185
CfoI GCGC 2 cut(s) 74, 968
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 1 cut(s) 648
Cfr42I CCGCGG 1 cut(s) 167
CseI GACGC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 32, 1053
CviAII CATG 7 cut(s) 63, 792, 834, 1040, 1070, 1166, 1184
CviQI GTAC 2 cut(s) 32, 1053
DdeI CTNAG 1 cut(s) 271
DpnI GATC 3 cut(s) 319, 353, 412
DpnII GATC 3 cut(s) 317, 351, 410
DraI TTTAAA 2 cut(s) 459, 777
DriI GACNNNNNGTC 1 cut(s) 1027
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 1165
Eco147I AGGCCT 1 cut(s) 181
Eco31I GGTCTC 2 cut(s) 129, 547
Eco47I GGWCC 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 39, 1059
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 648
EcoT14I CCWWGG 1 cut(s) 1165
EcoT22I ATGCAT 2 cut(s) 835, 1071
ErhI CCWWGG 1 cut(s) 1165
FaeI CATG 7 cut(s) 66, 795, 837, 1043, 1073, 1169, 1187
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 2 cut(s) 146, 634
FatI CATG 7 cut(s) 62, 791, 833, 1039, 1069, 1165, 1183
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 470, 629
FokI GGATG 2 cut(s) 824, 1019
Fsp4HI GCNGC 2 cut(s) 470, 629
FspBI CTAG 5 cut(s) 306, 348, 645, 905, 1026
GlaI GCGC 2 cut(s) 73, 967
GluI GCNGC 2 cut(s) 470, 629
GsuI CTGGAG 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 969
HaeIII GGCC 2 cut(s) 181, 1164
HapII CCGG 4 cut(s) 500, 624, 894, 909
HgaI GACGC 1 cut(s) 350
HhaI GCGC 2 cut(s) 74, 968
Hin1II CATG 7 cut(s) 66, 795, 837, 1043, 1073, 1169, 1187
Hin6I GCGC 2 cut(s) 72, 966
HinP1I GCGC 2 cut(s) 72, 966
HinfI GANTC 8 cut(s) 6, 159, 336, 521, 620, 845, 950, 1022
HpaII CCGG 4 cut(s) 500, 624, 894, 909
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 2 cut(s) 491, 721
Hpy188I TCNGA 4 cut(s) 811, 999, 1033, 1059
Hpy188III TCNNGA 5 cut(s) 10, 57, 256, 447, 500
Hpy8I GTNNAC 2 cut(s) 491, 721
HpyAV CCTTC 8 cut(s) 63, 131, 163, 271, 567, 664, 750, 1154
HpyCH4III ACNGT 4 cut(s) 107, 512, 878, 917
HpyCH4IV ACGT 1 cut(s) 717
HpyCH4V TGCA 7 cut(s) 206, 721, 833, 947, 976, 1039, 1069
HpyF10VI GCNNNNNNNGC 2 cut(s) 1184, 1189
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 717
Hsp92II CATG 7 cut(s) 66, 795, 837, 1043, 1073, 1169, 1187
HspAI GCGC 2 cut(s) 72, 966
Kpn2I TCCGGA 1 cut(s) 499
KspI CCGCGG 1 cut(s) 167
Kzo9I GATC 3 cut(s) 317, 351, 410
LmnI GCTCC 2 cut(s) 18, 59
Lsp1109I GCAGC 1 cut(s) 456
LweI GCATC 4 cut(s) 551, 667, 820, 934
MaeI CTAG 5 cut(s) 306, 348, 645, 905, 1026
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 3 cut(s) 872, 911, 1009
MalI GATC 3 cut(s) 319, 353, 412
MboI GATC 3 cut(s) 317, 351, 410
MboII GAAGA 5 cut(s) 271, 648, 932, 938, 1112
MhlI GDGCHC 1 cut(s) 723
MluCI AATT 5 cut(s) 454, 771, 787, 980, 985
MlyI GAGTC 3 cut(s) 168, 944, 1016
MmeI TCCRAC 1 cut(s) 1011
Mph1103I ATGCAT 2 cut(s) 835, 1071
MroI TCCGGA 1 cut(s) 499
MseI TTAA 6 cut(s) 458, 548, 776, 984, 1064, 1085
MslI CAYNNNNRTG 1 cut(s) 67
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 4 cut(s) 500, 624, 894, 909
MspR9I CCNGG 2 cut(s) 625, 895
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 2 cut(s) 1184, 1189
NciI CCSGG 2 cut(s) 625, 895
NcoI CCATGG 1 cut(s) 1165
NdeII GATC 3 cut(s) 317, 351, 410
NlaIII CATG 7 cut(s) 66, 795, 837, 1043, 1073, 1169, 1187
NlaIV GGNNCC 3 cut(s) 51, 650, 784
NmeAIII GCCGAG 1 cut(s) 518
NmuCI GTSAC 1 cut(s) 911
NsiI ATGCAT 2 cut(s) 835, 1071
NspI RCATGY 4 cut(s) 837, 1043, 1073, 1187
PaeI GCATGC 4 cut(s) 837, 1043, 1073, 1187
PceI AGGCCT 1 cut(s) 181
PfeI GAWTC 5 cut(s) 6, 336, 521, 620, 845
PkrI GCNGC 2 cut(s) 471, 630
PleI GAGTC 3 cut(s) 167, 944, 1016
PpsI GAGTC 3 cut(s) 167, 944, 1016
Ppu21I YACGTR 1 cut(s) 718
PpuMI RGGWCCY 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 648
PspN4I GGNNCC 3 cut(s) 51, 650, 784
PspPI GGNCC 1 cut(s) 648
PspPPI RGGWCCY 1 cut(s) 648
RsaI GTAC 2 cut(s) 33, 1054
RsaNI GTAC 2 cut(s) 32, 1053
RseI CAYNNNNRTG 1 cut(s) 67
SacII CCGCGG 1 cut(s) 167
SaqAI TTAA 6 cut(s) 458, 548, 776, 984, 1064, 1085
SatI GCNGC 2 cut(s) 470, 629
Sau3AI GATC 3 cut(s) 317, 351, 410
Sau96I GGNCC 1 cut(s) 648
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 3 cut(s) 168, 944, 1016
ScrFI CCNGG 2 cut(s) 625, 895
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 4 cut(s) 551, 667, 820, 934
Sfr303I CCGCGG 1 cut(s) 167
SgrBI CCGCGG 1 cut(s) 167
SinI GGWCC 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SphI GCATGC 4 cut(s) 837, 1043, 1073, 1187
Sse9I AATT 5 cut(s) 454, 771, 787, 980, 985
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 4 cut(s) 164, 166, 191, 628
SspI AATATT 1 cut(s) 752
SspMI CTAG 5 cut(s) 306, 348, 645, 905, 1026
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 623, 893
StyI CCWWGG 1 cut(s) 1165
TaaI ACNGT 4 cut(s) 107, 512, 878, 917
TaiI ACGT 1 cut(s) 720
TasI AATT 5 cut(s) 454, 771, 787, 980, 985
TatI WGTACW 1 cut(s) 31
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 5 cut(s) 6, 336, 521, 620, 845
Tru1I TTAA 6 cut(s) 458, 548, 776, 984, 1064, 1085
Tru9I TTAA 6 cut(s) 458, 548, 776, 984, 1064, 1085
TscAI CASTG 3 cut(s) 74, 112, 922
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 1 cut(s) 469
Tsp45I GTSAC 1 cut(s) 911
TspDTI ATGAA 5 cut(s) 303, 467, 780, 939, 1161
TspGWI ACGGA 1 cut(s) 1109
TspRI CASTG 3 cut(s) 74, 112, 922
VneI GTGCAC 1 cut(s) 719
VpaK11BI GGWCC 1 cut(s) 648
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 3 cut(s) 454, 771, 980
XceI RCATGY 4 cut(s) 837, 1043, 1073, 1187
XspI CTAG 5 cut(s) 306, 348, 645, 905, 1026
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 2 cut(s) 835, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.