FvH4_2g29660

ENT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003723
Physical Location & Seq
Forward (+)
22958710 .. 22962611
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29660.t1

Sequence Viewer

Length: 1146 bp
ATGAGATTCAAGAAGGGGGACAAAGTGGAAGTGCTAAGCCAAAAGGAGGTGCCTTCTGGAGCATGGCAGTGCGCTGTGATCGTCTCTGGCAATGGCCACAGTTATGGTGTTAGATATGATTGGTCTCCTAACGTGGGAGGTGAAGCAGTTGTGGAGAGAGTCCCGAGAAAGGCTATTAGGCCTTGCCCTCCGCCTGTTCAAGGTGCAGATAGTTGGGAGGTTGGCGATGTTGTTGAGGTCTTTGATGTTGGTTTCTGGAAGATGGCTAAGGTAGTGAAGGTTTATGATGAAAATTATTACCTGGCCAGGCTGCTTGGGTCATTCGAGGAGTTAAGGATCCACAAATCTTGGATCAGGGTGCGTCAATTGTTGAAAGATGATAAATGGATTGTGATTGGTAAGGTAAATTTAAGAATAAAGCTGCCGTCTGGAAATGGTGGCTTACCTTCTCCAGAAAACAGCAGTTGTCAGGATTCCTATATTGTCTCGTCTACTTCATCAAAGAGAGCTTGCCCATACACTGAAGAATATCCCCAGAAGATGAGAGCTATTGAGAAAGAGTGTGAGCATCAAGAAGTATTTTATAGGTCCCCATCTTCACTGCTAAAAAAGGTAGATAATGTTGCTTACCCGAGAGCAAAACTGGATGAAAAATACATGCGTGCTTCCTTTCTTTATCCTACAACTCGATATTTTGAAAAGGAGAACATAATTTCTAATGGTGCTAATTCATGTTGTGAAAGAAGCTCGGAAGTTAGTGATTGTGATACTGATGCATGCTCTATTGAGTCTGTTGGTAGTTGTAGTGTTAATGGTAACCATTCAAGTAAGATGGCTGGTTATGATTTAGCAGGTAATTGTGAAGAAGCGGATACCCTTTCTAGCGATGCAGACTCCTTTTCTAGTGGTGCAGATCTGGTTGAAAATGTTTCCCTTCCTCTGAAACTTGATATAGCAGCAACAATCCATAGGCTAGAGTTAGATGCTTATCGTCGAACTCTGATGGTAATGCATGCTTCGGGGTCCTTAAGTTGGGAACAACAAGATTTATTGACAACGCTCCGTATGTCTTTACACATTTCGAATGATGAACATTTGATGGAAGGCCAAGGTTGGAGGCTGCTTGCCTTGCTTCCAAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

382

Amino Acids

42.63

Weight (kDa)

5.85

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 6 - 64 2.4e-17 Agenet domain
ENT PF03735 320 - 366 1.9e-11 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 491
AciI CCGC 2 cut(s) 191, 869
AclWI GGATC 3 cut(s) 331, 344, 359
AcoI YGGCCR 2 cut(s) 94, 303
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 543
AfiI CCNNNNNNNGG 5 cut(s) 46, 134, 169, 200, 1032
AflII CTTAAG 1 cut(s) 1027
AgsI TTSAA 6 cut(s) 10, 200, 373, 698, 825, 923
AjnI CCWGG 2 cut(s) 300, 305
AluBI AGCT 4 cut(s) 421, 509, 548, 747
AluI AGCT 4 cut(s) 421, 509, 548, 747
Alw26I GTCTC 3 cut(s) 88, 129, 490
AlwI GGATC 3 cut(s) 331, 344, 359
Ama87I CYCGRG 2 cut(s) 163, 631
AoxI GGCC 4 cut(s) 94, 179, 303, 1105
ApeKI GCWGC 4 cut(s) 310, 421, 956, 1120
ApoI RAATTY 1 cut(s) 406
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 2 cut(s) 588, 1023
AsuHPI GGTGA 1 cut(s) 152
AsuII TTCGAA 1 cut(s) 1082
AvaI CYCGRG 2 cut(s) 163, 631
AvaII GGWCC 2 cut(s) 588, 1023
BalI TGGCCA 2 cut(s) 96, 305
BamHI GGATCC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 49
BbvI GCAGC 4 cut(s) 297, 408, 968, 1107
BccI CCATC 5 cut(s) 256, 601, 826, 997, 1093
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 2 cut(s) 302, 307
BciVI GTATCC 1 cut(s) 865
BcoDI GTCTC 3 cut(s) 88, 129, 490
BfaI CTAG 4 cut(s) 882, 903, 974, 1144
BfrI CTTAAG 1 cut(s) 1027
BfuAI ACCTGC 1 cut(s) 842
BfuI GTATCC 1 cut(s) 865
BglII AGATCT 1 cut(s) 913
BisI GCNGC 4 cut(s) 311, 422, 957, 1121
BlpI GCTNAGC 1 cut(s) 35
BlsI GCNGC 4 cut(s) 312, 423, 958, 1122
Bme1390I CCNGG 2 cut(s) 302, 307
Bme18I GGWCC 2 cut(s) 588, 1023
BmeT110I CYCGRG 2 cut(s) 163, 631
BmgT120I GGNCC 2 cut(s) 588, 1023
BmiI GGNNCC 4 cut(s) 51, 338, 590, 1024
BmrFI CCNGG 2 cut(s) 302, 307
BmsI GCATC 4 cut(s) 577, 763, 877, 973
BpmI CTGGAG 2 cut(s) 78, 435
Bpu10I CCTNAGC 1 cut(s) 267
Bpu1102I GCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 1082
BsaBI GATNNNNATC 1 cut(s) 987
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 1108
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 134, 169, 200, 1032
Bse1I ACTGG 1 cut(s) 648
Bse3DI GCAATG 1 cut(s) 97
Bse8I GATNNNNATC 1 cut(s) 987
BseBI CCWGG 2 cut(s) 302, 307
BseDI CCNNGG 1 cut(s) 1108
BseGI GGATG 1 cut(s) 652
BseJI GATNNNNATC 1 cut(s) 987
BseLI CCNNNNNNNGG 5 cut(s) 46, 134, 169, 200, 1032
BseMI GCAATG 1 cut(s) 97
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 4 cut(s) 297, 408, 968, 1107
BsgI GTGCAG 2 cut(s) 225, 930
BshFI GGCC 4 cut(s) 96, 181, 305, 1107
BshNI GGYRCC 1 cut(s) 49
BsiHKCI CYCGRG 2 cut(s) 163, 631
BslFI GGGAC 3 cut(s) 32, 146, 574
BslI CCNNNNNNNGG 5 cut(s) 46, 134, 169, 200, 1032
BsmAI GTCTC 3 cut(s) 88, 129, 490
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 3 cut(s) 32, 146, 574
BsnI GGCC 4 cut(s) 96, 181, 305, 1107
Bso31I GGTCTC 1 cut(s) 129
BsoBI CYCGRG 2 cut(s) 163, 631
Bsp119I TTCGAA 1 cut(s) 1082
Bsp143I GATC 4 cut(s) 78, 336, 351, 913
Bsp1720I GCTNAGC 1 cut(s) 35
BspACI CCGC 2 cut(s) 191, 869
BspANI GGCC 4 cut(s) 96, 181, 305, 1107
BspLI GGNNCC 4 cut(s) 51, 338, 590, 1024
BspMI ACCTGC 1 cut(s) 842
BspPI GGATC 3 cut(s) 331, 344, 359
BspT104I TTCGAA 1 cut(s) 1082
BspT107I GGYRCC 1 cut(s) 49
BspTI CTTAAG 1 cut(s) 1027
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 1 cut(s) 648
BssECI CCNNGG 1 cut(s) 1108
BssMI GATC 4 cut(s) 78, 336, 351, 913
BssT1I CCWWGG 1 cut(s) 1108
Bst2UI CCWGG 2 cut(s) 302, 307
Bst4CI ACNGT 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 1027
BstBI TTCGAA 1 cut(s) 1082
BstC8I GCNNGC 5 cut(s) 511, 663, 778, 1014, 1125
BstDEI CTNAG 2 cut(s) 35, 267
BstEII GGTNACC 1 cut(s) 815
BstENI CCTNNNNNAGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 652
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 81, 339, 354, 916
BstMAI GTCTC 3 cut(s) 88, 129, 490
BstMBI GATC 4 cut(s) 78, 336, 351, 913
BstMWI GCNNNNNNNGC 1 cut(s) 1129
BstNI CCWGG 2 cut(s) 302, 307
BstNSI RCATGY 3 cut(s) 661, 780, 1016
BstPI GGTNACC 1 cut(s) 815
BstSCI CCNGG 2 cut(s) 300, 305
BstV1I GCAGC 4 cut(s) 297, 408, 968, 1107
BstX2I RGATCY 2 cut(s) 336, 913
BstXI CCANNNNNNTGG 1 cut(s) 104
BstYI RGATCY 2 cut(s) 336, 913
BsuI GTATCC 1 cut(s) 865
BsuRI GGCC 4 cut(s) 96, 181, 305, 1107
BtgZI GCGATG 2 cut(s) 240, 900
BtsCI GGATG 1 cut(s) 652
BtsI GCAGTG 2 cut(s) 74, 599
BtsIMutI CAGTG 3 cut(s) 74, 519, 599
BveI ACCTGC 1 cut(s) 842
Cac8I GCNNGC 5 cut(s) 511, 663, 778, 1014, 1125
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 588, 1023
CseI GACGC 1 cut(s) 350
CspCI CAANNNNNGTGG 2 cut(s) 329, 364
CviAII CATG 5 cut(s) 63, 658, 732, 777, 1013
DdeI CTNAG 2 cut(s) 35, 267
DpnI GATC 4 cut(s) 80, 338, 353, 915
DpnII GATC 4 cut(s) 78, 336, 351, 913
EaeI YGGCCR 2 cut(s) 94, 303
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 1108
Eco147I AGGCCT 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 588, 1023
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 2 cut(s) 163, 631
Eco91I GGTNACC 1 cut(s) 815
EcoNI CCTNNNNNAGG 1 cut(s) 198
EcoO109I RGGNCCY 2 cut(s) 588, 1023
EcoO65I GGTNACC 1 cut(s) 815
EcoRII CCWGG 2 cut(s) 300, 305
EcoT14I CCWWGG 1 cut(s) 1108
EcoT22I ATGCAT 2 cut(s) 778, 1014
ErhI CCWWGG 1 cut(s) 1108
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 5 cut(s) 66, 661, 735, 780, 1016
FaqI GGGAC 3 cut(s) 32, 146, 574
FatI CATG 5 cut(s) 62, 657, 731, 776, 1012
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 4 cut(s) 311, 422, 957, 1121
FokI GGATG 1 cut(s) 659
Fsp4HI GCNGC 4 cut(s) 311, 422, 957, 1121
FspBI CTAG 4 cut(s) 882, 903, 974, 1144
GlaI GCGC 1 cut(s) 73
GluI GCNGC 4 cut(s) 311, 422, 957, 1121
GsuI CTGGAG 2 cut(s) 78, 435
HaeIII GGCC 4 cut(s) 96, 181, 305, 1107
HgaI GACGC 1 cut(s) 350
HhaI GCGC 1 cut(s) 74
Hin1II CATG 5 cut(s) 66, 661, 735, 780, 1016
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 5 cut(s) 6, 159, 473, 788, 893
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 492
Hpy188I TCNGA 3 cut(s) 751, 942, 1002
Hpy188III TCNNGA 8 cut(s) 10, 57, 163, 256, 429, 452, 470, 572
Hpy8I GTNNAC 1 cut(s) 492
Hpy99I CGWCG 1 cut(s) 996
HpyAV CCTTC 6 cut(s) 7, 63, 271, 456, 944, 1097
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 206, 776, 890, 911, 1012
HpyF10VI GCNNNNNNNGC 1 cut(s) 1129
HpyF3I CTNAG 2 cut(s) 35, 267
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 5 cut(s) 66, 661, 735, 780, 1016
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 78, 336, 351, 913
LmnI GCTCC 2 cut(s) 59, 1065
Lsp1109I GCAGC 4 cut(s) 297, 408, 968, 1107
LweI GCATC 4 cut(s) 577, 763, 877, 973
MaeI CTAG 4 cut(s) 882, 903, 974, 1144
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 815
MalI GATC 4 cut(s) 80, 338, 353, 915
MboI GATC 4 cut(s) 78, 336, 351, 913
MboII GAAGA 5 cut(s) 271, 536, 550, 588, 875
MfeI CAATTG 1 cut(s) 365
MflI RGATCY 2 cut(s) 336, 913
MlsI TGGCCA 2 cut(s) 96, 305
MluCI AATT 6 cut(s) 292, 365, 406, 711, 727, 856
MluNI TGGCCA 2 cut(s) 96, 305
MlyI GAGTC 3 cut(s) 168, 797, 887
MmeI TCCRAC 1 cut(s) 1094
MnlI CCTC 8 cut(s) 40, 131, 198, 211, 229, 319, 948, 1110
Mox20I TGGCCA 2 cut(s) 96, 305
Mph1103I ATGCAT 2 cut(s) 778, 1014
MscI TGGCCA 2 cut(s) 96, 305
MseI TTAA 4 cut(s) 332, 410, 810, 1028
MslI CAYNNNNRTG 2 cut(s) 67, 102
Msp20I TGGCCA 2 cut(s) 96, 305
MspCI CTTAAG 1 cut(s) 1027
MspR9I CCNGG 2 cut(s) 302, 307
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 2 cut(s) 302, 307
MwoI GCNNNNNNNGC 1 cut(s) 1129
NdeII GATC 4 cut(s) 78, 336, 351, 913
NlaIII CATG 5 cut(s) 66, 661, 735, 780, 1016
NlaIV GGNNCC 4 cut(s) 51, 338, 590, 1024
NsiI ATGCAT 2 cut(s) 778, 1014
NspI RCATGY 3 cut(s) 661, 780, 1016
NspV TTCGAA 1 cut(s) 1082
PaeI GCATGC 2 cut(s) 780, 1016
PceI AGGCCT 1 cut(s) 181
PfeI GAWTC 2 cut(s) 6, 473
PkrI GCNGC 4 cut(s) 312, 423, 958, 1122
PleI GAGTC 3 cut(s) 167, 796, 887
PpsI GAGTC 3 cut(s) 167, 796, 887
PpuMI RGGWCCY 2 cut(s) 588, 1023
Psp5II RGGWCCY 2 cut(s) 588, 1023
Psp6I CCWGG 2 cut(s) 300, 305
PspEI GGTNACC 1 cut(s) 815
PspGI CCWGG 2 cut(s) 300, 305
PspN4I GGNNCC 4 cut(s) 51, 338, 590, 1024
PspPI GGNCC 2 cut(s) 588, 1023
PspPPI RGGWCCY 2 cut(s) 588, 1023
PsuI RGATCY 2 cut(s) 336, 913
RseI CAYNNNNRTG 2 cut(s) 67, 102
SaqAI TTAA 4 cut(s) 332, 410, 810, 1028
SatI GCNGC 4 cut(s) 311, 422, 957, 1121
Sau3AI GATC 4 cut(s) 78, 336, 351, 913
Sau96I GGNCC 2 cut(s) 588, 1023
SchI GAGTC 3 cut(s) 168, 797, 887
ScrFI CCNGG 2 cut(s) 302, 307
SfaNI GCATC 4 cut(s) 577, 763, 877, 973
SfuI TTCGAA 1 cut(s) 1082
SinI GGWCC 2 cut(s) 588, 1023
SmiMI CAYNNNNRTG 2 cut(s) 67, 102
SmlI CTYRAG 1 cut(s) 1027
SmoI CTYRAG 1 cut(s) 1027
SphI GCATGC 2 cut(s) 780, 1016
Sse9I AATT 6 cut(s) 292, 365, 406, 711, 727, 856
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 2 cut(s) 191, 869
SspMI CTAG 4 cut(s) 882, 903, 974, 1144
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 300, 305
StyI CCWWGG 1 cut(s) 1108
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 324, 688, 994, 1082
TasI AATT 6 cut(s) 292, 365, 406, 711, 727, 856
TfiI GAWTC 2 cut(s) 6, 473
Tru1I TTAA 4 cut(s) 332, 410, 810, 1028
Tru9I TTAA 4 cut(s) 332, 410, 810, 1028
TscAI CASTG 3 cut(s) 74, 526, 606
TseI GCWGC 4 cut(s) 310, 421, 956, 1120
TspDTI ATGAA 5 cut(s) 303, 486, 663, 720, 1104
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 3 cut(s) 74, 526, 606
Vha464I CTTAAG 1 cut(s) 1027
VpaK11BI GGWCC 2 cut(s) 588, 1023
XagI CCTNNNNNAGG 1 cut(s) 198
XapI RAATTY 1 cut(s) 406
XceI RCATGY 3 cut(s) 661, 780, 1016
XmiI GTMKAC 1 cut(s) 491
XspI CTAG 4 cut(s) 882, 903, 974, 1144
Zsp2I ATGCAT 2 cut(s) 778, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.