RLG00000001759

ENT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003723
Physical Location & Seq
Reverse (-)
20226663 .. 20232882
6220 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001759

Sequence Viewer

Length: 1350 bp
ATGTTGTTAAGATCGATATCAATATTCTTCTCATCCCAGTCGAATTTTGTAACATCGCAAGGCAGGGTCTCAGGATCTTACATTGCAATCATGAGATTCAAGAAAGGGAGCAAGGTGGAAGTACTGAACGAGAAGGAGGTGCCTTCAGGAGCATGGCAGTGCGCTGTGGTTATATCAGGCAATGGACACACTTACAGTGTTAGGTATGATTGGTCTCCTAACTGGGAAGGTCAAGCAGTTATTGAGAGAGTCCCGCGGAAGGCTATTAGGCCTTGTCCTCCGCCTGTTCAAGGTGCAGATTGTTGGGCAGTCGGTGATGTTGTTGAGGTCTTTGATGTTGGTTTCTGGAAGATGGCTAACATAGTGAAGGGTTTTGATGAAAACTACTACTTGGCTAGGCTACTTGGATCATTTGAGGAGTTTAGAATCCACAAATCTAGGATCAGGGTGCGTCAAGTTTTGAAAGATGAGGAATGGATTGTGATAGGAAAGGGTCCAGGATCAGCTTACCACAATCATACGAGTTCTCCAGTTTCACGAATGAATTTAAGAATAAAGCTGCCCAGTGGAAATGATTGTTTACCTCCTCCAGAAAACAACAGTTGTGAGGATTCCCATATTGTCTCCTCGGCATCATTAAAGAGACCTTCTCAATGTTGCTCTCTTTATACCGAAGCATATCCCCGAAAGATAAGAGCTATTGAGAAAGAATCCGGGCGGCAACGAGCTTTCTCTAGGTCCCCATCTTCCTTGCTTGAGAAGGTAGATGCTGTTGCTTACCCAAGAAAAAATCTGGCTGAAAAATACGTGCACGCTTCCTTTATTTGCCCTTCCACTCAATATAGTGAAAATGAGAAGGGAATTTTAAATGGTGCCAATTCATGGTTTGAAAGAAGCTCGGATGGTGTTTTTGATAGTGATGTATGCTCTATTGAATCTGTTGGTAGTTGTAGTGTTATTAGTAACAGTTCAAATAAGTTGTCCGGGTATAATCTAGCAGGTCACAGTGAAGATGAAGATACCCTTTGTAGTGATGCAGACTCCTTTTCTGGTGGCGCTGATGTGCAAGAATTTAATTCCTTTCCTCTGAAAGGGGATATAACAGCAAGAGACTCTAGGTCGGAGTTGCATGCGTATCGCCGTACTCTGATGTTAATGCATGCTTCAGGTTCTTTAAGTTGGGAACAAGAAGATTTATTGACAAACCTCCGGCCATGGTTGGGAGGCTTGCCGTGCCTTGCTTCCAAAGTTCTAAATATGGTGGTTCTTAAATATGGTTCAGTTTGGGTCTGCTGGGAATTGTTGACACATCTTGTGCCAAAGCAGAAGAGAACTGAAAAGTGCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

450

Amino Acids

50.11

Weight (kDa)

8.12

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 36 - 94 2.4e-16 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 989
AccB1I GGYRCC 2 cut(s) 139, 872
AccB7I CCANNNNNTGG 1 cut(s) 882
AccII CGCG 1 cut(s) 256
AciI CCGC 4 cut(s) 254, 256, 281, 718
AclWI GGATC 4 cut(s) 82, 415, 449, 508
AcoI YGGCCR 1 cut(s) 1211
AcsI RAATTY 4 cut(s) 43, 544, 861, 1070
AcuI CTGAAG 2 cut(s) 129, 1149
AfaI GTAC 2 cut(s) 123, 1144
AfiI CCNNNNNNNGG 5 cut(s) 259, 290, 882, 1091, 1220
AgsI TTSAA 6 cut(s) 100, 290, 463, 890, 935, 972
AhdI GACNNNNNGTC 1 cut(s) 1117
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 5 cut(s) 506, 559, 698, 728, 897
AluI AGCT 5 cut(s) 506, 559, 698, 728, 897
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 5 cut(s) 73, 219, 628, 637, 1104
Alw44I GTGCAC 1 cut(s) 809
AlwI GGATC 4 cut(s) 82, 415, 449, 508
AoxI GGCC 2 cut(s) 269, 1211
ApaLI GTGCAC 1 cut(s) 809
ApeKI GCWGC 1 cut(s) 559
ApoI RAATTY 4 cut(s) 43, 544, 861, 1070
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
AspLEI GCGC 2 cut(s) 164, 1058
AspS9I GGNCC 2 cut(s) 494, 738
AsuC2I CCSGG 2 cut(s) 715, 985
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 2 cut(s) 494, 738
BaeGI GKGCMC 1 cut(s) 813
BanI GGYRCC 2 cut(s) 139, 872
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 1 cut(s) 546
BccI CCATC 3 cut(s) 346, 751, 896
BceAI ACGGC 2 cut(s) 1125, 1216
BcgI CGANNNNNNTGC 2 cut(s) 1118, 1152
BciT130I CCWGG 1 cut(s) 498
BcnI CCSGG 2 cut(s) 715, 985
BcoDI GTCTC 5 cut(s) 73, 219, 628, 637, 1104
BfaI CTAG 5 cut(s) 396, 438, 735, 995, 1116
BfoI RGCGCY 1 cut(s) 1059
BfuAI ACCTGC 1 cut(s) 989
BisI GCNGC 2 cut(s) 560, 719
BlsI GCNGC 2 cut(s) 561, 720
BmcAI AGTACT 1 cut(s) 123
Bme1390I CCNGG 3 cut(s) 498, 715, 985
Bme18I GGWCC 2 cut(s) 494, 738
BmeRI GACNNNNNGTC 1 cut(s) 1117
BmgT120I GGNCC 2 cut(s) 494, 738
BmiI GGNNCC 4 cut(s) 141, 495, 740, 874
BmrFI CCNGG 3 cut(s) 498, 715, 985
BmrI ACTGGG 3 cut(s) 31, 232, 558
BmsI GCATC 3 cut(s) 641, 757, 1024
BmuI ACTGGG 3 cut(s) 31, 232, 558
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpmI CTGGAG 2 cut(s) 513, 573
BpuEI CTTGAG 1 cut(s) 776
BpuMI CCSGG 2 cut(s) 715, 985
Bsa29I ATCGAT 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 808
BsaBI GATNNNNATC 1 cut(s) 16
BsaI GGTCTC 3 cut(s) 73, 219, 637
BsaJI CCNNGG 3 cut(s) 254, 627, 1214
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 290, 882, 1091, 1220
Bse1I ACTGG 4 cut(s) 37, 227, 530, 564
Bse3DI GCAATG 2 cut(s) 81, 187
Bse8I GATNNNNATC 1 cut(s) 16
BseBI CCWGG 1 cut(s) 498
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 254, 627, 1214
BseGI GGATG 2 cut(s) 32, 907
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 5 cut(s) 259, 290, 882, 1091, 1220
BseMI GCAATG 2 cut(s) 81, 187
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 4 cut(s) 37, 227, 530, 564
BseRI GAGGAG 3 cut(s) 431, 576, 616
BseSI GKGCMC 1 cut(s) 813
BseXI GCAGC 1 cut(s) 546
BseYI CCCAGC 1 cut(s) 1293
BsgI GTGCAG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 271, 1213
BshNI GGYRCC 2 cut(s) 139, 872
BshVI ATCGAT 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 813
BsiSI CCGG 3 cut(s) 714, 984, 1210
BslFI GGGAC 2 cut(s) 236, 724
BslI CCNNNNNNNGG 5 cut(s) 259, 290, 882, 1091, 1220
BsmAI GTCTC 5 cut(s) 73, 219, 628, 637, 1104
BsmFI GGGAC 2 cut(s) 236, 724
BsnI GGCC 2 cut(s) 271, 1213
Bso31I GGTCTC 3 cut(s) 73, 219, 637
Bsp1286I GDGCHC 1 cut(s) 813
Bsp143I GATC 5 cut(s) 11, 74, 407, 441, 500
Bsp19I CCATGG 1 cut(s) 1214
BspACI CCGC 4 cut(s) 254, 256, 281, 718
BspANI GGCC 2 cut(s) 271, 1213
BspCNI CTCAG 1 cut(s) 83
BspDI ATCGAT 1 cut(s) 14
BspFNI CGCG 1 cut(s) 256
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 4 cut(s) 141, 495, 740, 874
BspMI ACCTGC 1 cut(s) 989
BspPI GGATC 4 cut(s) 82, 415, 449, 508
BspT107I GGYRCC 2 cut(s) 139, 872
BspTNI GGTCTC 3 cut(s) 73, 219, 637
BsrDI GCAATG 2 cut(s) 81, 187
BsrI ACTGG 4 cut(s) 37, 227, 530, 564
BssECI CCNNGG 3 cut(s) 254, 627, 1214
BssMI GATC 5 cut(s) 11, 74, 407, 441, 500
BssT1I CCWWGG 1 cut(s) 1214
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 4 cut(s) 197, 602, 968, 1007
Bst6I CTCTTC 1 cut(s) 1322
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 4 cut(s) 813, 1131, 1161, 1229
BstDEI CTNAG 1 cut(s) 70
BstDSI CCRYGG 2 cut(s) 254, 1214
BstENI CCTNNNNNAGG 2 cut(s) 288, 1089
BstF5I GGATG 2 cut(s) 32, 907
BstFNI CGCG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 1059
BstHHI GCGC 2 cut(s) 164, 1058
BstKTI GATC 5 cut(s) 14, 77, 410, 444, 503
BstMAI GTCTC 5 cut(s) 73, 219, 628, 637, 1104
BstMBI GATC 5 cut(s) 11, 74, 407, 441, 500
BstMWI GCNNNNNNNGC 1 cut(s) 1233
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 2 cut(s) 1133, 1163
BstSCI CCNGG 3 cut(s) 496, 713, 983
BstSLI GKGCMC 1 cut(s) 813
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 14
BsuRI GGCC 2 cut(s) 271, 1213
BsuTUI ATCGAT 1 cut(s) 14
BtgI CCRYGG 2 cut(s) 254, 1214
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 32, 907
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 4 cut(s) 164, 202, 571, 1012
BveI ACCTGC 1 cut(s) 989
Cac8I GCNNGC 4 cut(s) 813, 1131, 1161, 1229
CciI TCATGA 1 cut(s) 90
CfoI GCGC 2 cut(s) 164, 1058
Cfr13I GGNCC 2 cut(s) 494, 738
Cfr42I CCGCGG 1 cut(s) 257
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 122, 1143
CviAII CATG 6 cut(s) 91, 153, 882, 1130, 1160, 1215
CviQI GTAC 2 cut(s) 122, 1143
DdeI CTNAG 1 cut(s) 70
DpnI GATC 5 cut(s) 13, 76, 409, 443, 502
DpnII GATC 5 cut(s) 11, 74, 407, 441, 500
DraI TTTAAA 1 cut(s) 867
DriI GACNNNNNGTC 1 cut(s) 1117
EaeI YGGCCR 1 cut(s) 1211
Eam1104I CTCTTC 1 cut(s) 1322
Eam1105I GACNNNNNGTC 1 cut(s) 1117
EarI CTCTTC 1 cut(s) 1322
EciI GGCGGA 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 1214
Eco147I AGGCCT 1 cut(s) 271
Eco31I GGTCTC 3 cut(s) 73, 219, 637
Eco32I GATATC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 494, 738
Eco57I CTGAAG 2 cut(s) 129, 1149
EcoNI CCTNNNNNAGG 2 cut(s) 288, 1089
EcoO109I RGGNCCY 1 cut(s) 738
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 1214
EcoT22I ATGCAT 1 cut(s) 1161
ErhI CCWWGG 1 cut(s) 1214
FaeI CATG 6 cut(s) 94, 156, 885, 1133, 1163, 1218
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FaqI GGGAC 2 cut(s) 236, 724
FatI CATG 6 cut(s) 90, 152, 881, 1129, 1159, 1214
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 560, 719
FokI GGATG 2 cut(s) 19, 914
Fsp4HI GCNGC 2 cut(s) 560, 719
FspBI CTAG 5 cut(s) 396, 438, 735, 995, 1116
GlaI GCGC 2 cut(s) 163, 1057
GluI GCNGC 2 cut(s) 560, 719
GsaI CCCAGC 1 cut(s) 1297
GsuI CTGGAG 2 cut(s) 513, 573
HaeII RGCGCY 1 cut(s) 1059
HaeIII GGCC 2 cut(s) 271, 1213
HapII CCGG 3 cut(s) 714, 984, 1210
HgaI GACGC 1 cut(s) 440
HhaI GCGC 2 cut(s) 164, 1058
Hin1II CATG 6 cut(s) 94, 156, 885, 1133, 1163, 1218
Hin6I GCGC 2 cut(s) 162, 1056
HinP1I GCGC 2 cut(s) 162, 1056
HincII GTYRAC 1 cut(s) 1305
HindII GTYRAC 1 cut(s) 1305
HinfI GANTC 8 cut(s) 96, 249, 426, 611, 710, 935, 1040, 1112
HpaII CCGG 3 cut(s) 714, 984, 1210
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 581, 811, 1305
Hpy188I TCNGA 4 cut(s) 901, 1089, 1123, 1149
Hpy188III TCNNGA 7 cut(s) 72, 91, 100, 147, 346, 537, 590
Hpy8I GTNNAC 3 cut(s) 581, 811, 1305
HpyAV CCTTC 9 cut(s) 127, 153, 221, 253, 361, 657, 754, 840, 850
HpyCH4III ACNGT 4 cut(s) 197, 602, 968, 1007
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 7 cut(s) 86, 296, 811, 1037, 1066, 1129, 1159
HpyF10VI GCNNNNNNNGC 1 cut(s) 1233
HpyF3I CTNAG 1 cut(s) 70
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 6 cut(s) 94, 156, 885, 1133, 1163, 1218
HspAI GCGC 2 cut(s) 162, 1056
KspI CCGCGG 1 cut(s) 257
Kzo9I GATC 5 cut(s) 11, 74, 407, 441, 500
LmnI GCTCC 2 cut(s) 108, 149
Lsp1109I GCAGC 1 cut(s) 546
LweI GCATC 3 cut(s) 641, 757, 1024
MaeI CTAG 5 cut(s) 396, 438, 735, 995, 1116
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 3 cut(s) 49, 962, 1001
MalI GATC 5 cut(s) 13, 76, 409, 443, 502
MboI GATC 5 cut(s) 11, 74, 407, 441, 500
MboII GAAGA 7 cut(s) 19, 361, 738, 1022, 1028, 1202, 1339
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 813
MluCI AATT 7 cut(s) 43, 544, 861, 877, 1070, 1075, 1298
MlyI GAGTC 3 cut(s) 258, 1034, 1106
MmeI TCCRAC 1 cut(s) 1101
Mph1103I ATGCAT 1 cut(s) 1161
MseI TTAA 8 cut(s) 8, 548, 638, 866, 1074, 1154, 1175, 1269
MslI CAYNNNNRTG 1 cut(s) 157
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 3 cut(s) 714, 984, 1210
MspR9I CCNGG 3 cut(s) 498, 715, 985
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 1233
NciI CCSGG 2 cut(s) 715, 985
NcoI CCATGG 1 cut(s) 1214
NdeII GATC 5 cut(s) 11, 74, 407, 441, 500
NlaIII CATG 6 cut(s) 94, 156, 885, 1133, 1163, 1218
NlaIV GGNNCC 4 cut(s) 141, 495, 740, 874
NmeAIII GCCGAG 1 cut(s) 608
NmuCI GTSAC 1 cut(s) 1001
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 2 cut(s) 1133, 1163
PaeI GCATGC 2 cut(s) 1133, 1163
PagI TCATGA 1 cut(s) 90
PceI AGGCCT 1 cut(s) 271
PfeI GAWTC 5 cut(s) 96, 426, 611, 710, 935
PflMI CCANNNNNTGG 1 cut(s) 882
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 2 cut(s) 561, 720
PleI GAGTC 3 cut(s) 257, 1034, 1106
PpsI GAGTC 3 cut(s) 257, 1034, 1106
Ppu21I YACGTR 1 cut(s) 808
PpuMI RGGWCCY 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 738
Psp6I CCWGG 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 1293
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 4 cut(s) 141, 495, 740, 874
PspPI GGNCC 2 cut(s) 494, 738
PspPPI RGGWCCY 1 cut(s) 738
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 2 cut(s) 123, 1144
RsaNI GTAC 2 cut(s) 122, 1143
RseI CAYNNNNRTG 1 cut(s) 157
SacII CCGCGG 1 cut(s) 257
SaqAI TTAA 8 cut(s) 8, 548, 638, 866, 1074, 1154, 1175, 1269
SatI GCNGC 2 cut(s) 560, 719
Sau3AI GATC 5 cut(s) 11, 74, 407, 441, 500
Sau96I GGNCC 2 cut(s) 494, 738
ScaI AGTACT 1 cut(s) 123
SchI GAGTC 3 cut(s) 258, 1034, 1106
ScrFI CCNGG 3 cut(s) 498, 715, 985
SduI GDGCHC 1 cut(s) 813
SfaNI GCATC 3 cut(s) 641, 757, 1024
Sfr303I CCGCGG 1 cut(s) 257
SgrBI CCGCGG 1 cut(s) 257
SinI GGWCC 2 cut(s) 494, 738
SmiMI CAYNNNNRTG 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 755
SmoI CTYRAG 1 cut(s) 755
SphI GCATGC 2 cut(s) 1133, 1163
Sse9I AATT 7 cut(s) 43, 544, 861, 877, 1070, 1075, 1298
SseBI AGGCCT 1 cut(s) 271
SsiI CCGC 4 cut(s) 254, 256, 281, 718
SspI AATATT 1 cut(s) 24
SspMI CTAG 5 cut(s) 396, 438, 735, 995, 1116
StuI AGGCCT 1 cut(s) 271
StyD4I CCNGG 3 cut(s) 496, 713, 983
StyI CCWWGG 1 cut(s) 1214
TaaI ACNGT 4 cut(s) 197, 602, 968, 1007
TaiI ACGT 1 cut(s) 810
TaqI TCGA 2 cut(s) 14, 41
TasI AATT 7 cut(s) 43, 544, 861, 877, 1070, 1075, 1298
TatI WGTACW 1 cut(s) 121
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 5 cut(s) 96, 426, 611, 710, 935
Tru1I TTAA 8 cut(s) 8, 548, 638, 866, 1074, 1154, 1175, 1269
Tru9I TTAA 8 cut(s) 8, 548, 638, 866, 1074, 1154, 1175, 1269
TscAI CASTG 4 cut(s) 164, 202, 571, 1012
TseFI GTSAC 1 cut(s) 1001
TseI GCWGC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 1001
TspDTI ATGAA 4 cut(s) 393, 557, 870, 1029
TspRI CASTG 4 cut(s) 164, 202, 571, 1012
Van91I CCANNNNNTGG 1 cut(s) 882
VneI GTGCAC 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 494, 738
XagI CCTNNNNNAGG 2 cut(s) 288, 1089
XapI RAATTY 4 cut(s) 43, 544, 861, 1070
XceI RCATGY 2 cut(s) 1133, 1163
XspI CTAG 5 cut(s) 396, 438, 735, 995, 1116
ZrmI AGTACT 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.