Prupe.1G473100_v2.0.a1

ENT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0003723
Physical Location & Seq
Reverse (-)
39320367 .. 39325383
5017 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G473100.1

Sequence Viewer

Length: 1209 bp
ATGAGATTCAAGAAAGGAAATAAAGTGGAGATACTTAGCCAAAAGGAGGTGCCTTCAGTAGCATGGCGTTGCGCTGAGATTATCTCAGGTAATGGGCACACCTACAGTGTTAAGTATGATCGGTCTCCTGGTAAGGATAGTCAAGCAGTTGTGGAGAGAGTTCCAAGGAAGGATATTAGGCCTTGCCCTCCACCTGTTCAAAGTGCGGAGATTTTGGCAGTTGGTGATGTTGCTGAGGTGTTCGATGCTGGTTTCTGGAAAATGGCTAAGGTGTTGAAGGTTTTGGATGAAAATTATTACTTGACTAGGCTACTTGGATCTTCTGAGGAGTTTAGAGTGCACAAACATAGGATCCGGGTGCGCCAATTTTGGAAAGATGACAAATGGGTTGTGATCGGAAAGGGTTCTGATAATCGTATTAGCTCGCAAGTCCCACAATTGAATGCAAGAATAAAGCTACCAGCTGTGAATGATTGTGTACCTCCACTGGACATCATCAGTTATCAGGATTCCCATAATGTCTCATCGAGATCATTGAAGAGAGCTTCTCCATATTGCTCATTTTATATTGATGGATATTCACGAAAGAGGAGAGCAATTGAGAAGGGGTGTGAGAGCCAACACTTTTTTTCTGGGTCCCCATCTTCCTTTCTCAAAAAGGTAGATGCTGTTGCTTACCCACGAGAAAATCTGGGTGAAAAATATGTGCAGGCTTCCTTTATTAATCGTACAACTGGATGTTTTGAAAAGGAGAGGGAAGAGTTAAATGGTGCTATTTCTCTTTCTGTTGAAAGAAGTTCAGAACTTGATGATTGTGATAGTGATGCATGCTCTGTGGGTAGTTGTAGTGTTATTAGTAGCAATTCAAATAAGTTTTCGAGTCATAATTTAGCAGGTACTAGTCAAGATTCAGATACCCTTTCTAGTGATGCAGATTCTTTTTGTGGAGATGCGCAAGAAAAATTTTCCGTTCCTCTGGAAGAGGATGTACCAGCAGGAATCCATAGGTTAGAGTTGAATGCTTATCACAGTACTCTGGTGGCAATGCATGCTTCTGGATCCTTAACTTGGGAACAAGAAGCGTTATTGACAAATCTCCGTATTTCACTGCACATTTCAAACGATGAGCATTTGATTGAAGTTCACCATGCTGCTGTCTTTTCATCTGTTCTTAATAATGTCTCTTCACCAGTTTTTTCTCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet ENT

Protein Analysis

403

Amino Acids

44.72

Weight (kDa)

6.37

Isoelectric Point (pI)

56.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 954
Acc36I ACCTGC 1 cut(s) 884
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 1 cut(s) 206
AclWI GGATC 5 cut(s) 325, 346, 359, 1053, 1066
AcsI RAATTY 1 cut(s) 962
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 5 cut(s) 480, 730, 898, 990, 1033
AfiI CCNNNNNNNGG 2 cut(s) 46, 1068
AhlI ACTAGT 1 cut(s) 899
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 4 cut(s) 423, 457, 464, 545
AluI AGCT 4 cut(s) 423, 457, 464, 545
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 3 cut(s) 129, 526, 1186
Alw44I GTGCAC 1 cut(s) 338
AlwI GGATC 5 cut(s) 325, 346, 359, 1053, 1066
AoxI GGCC 1 cut(s) 179
ApaLI GTGCAC 1 cut(s) 338
ApeKI GCWGC 1 cut(s) 1151
ApoI RAATTY 1 cut(s) 962
AseI ATTAAT 1 cut(s) 723
Asp700I GAANNNNTTC 1 cut(s) 403
AspLEI GCGC 3 cut(s) 74, 363, 955
AspS9I GGNCC 1 cut(s) 636
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 4 cut(s) 236, 707, 1136, 1179
AvaII GGWCC 1 cut(s) 636
BaeGI GKGCMC 2 cut(s) 99, 342
BamHI GGATCC 2 cut(s) 351, 1058
BanI GGYRCC 1 cut(s) 49
BarI GAAGNNNNNNTAC 2 cut(s) 972, 1004
BauI CACGAG 1 cut(s) 681
Bbv12I GWGCWC 1 cut(s) 342
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 1138
BccI CCATC 2 cut(s) 566, 649
BciT130I CCWGG 1 cut(s) 129
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 3 cut(s) 129, 526, 1186
BcuI ACTAGT 1 cut(s) 899
BfaI CTAG 3 cut(s) 306, 900, 924
BfmI CTRYAG 1 cut(s) 103
BfuAI ACCTGC 1 cut(s) 884
BisI GCNGC 1 cut(s) 1152
BlsI GCNGC 1 cut(s) 1153
BmcAI AGTACT 1 cut(s) 1033
Bme1390I CCNGG 2 cut(s) 129, 356
Bme18I GGWCC 1 cut(s) 636
BmgT120I GGNCC 1 cut(s) 636
BmiI GGNNCC 5 cut(s) 51, 353, 637, 638, 1060
BmrFI CCNGG 2 cut(s) 129, 356
BmsI GCATC 5 cut(s) 235, 655, 814, 919, 940
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 532, 564
Bpu10I CCTNAGC 2 cut(s) 234, 267
BpuMI CCSGG 1 cut(s) 356
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 164
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 1068
Bse1I ACTGG 3 cut(s) 492, 739, 1190
Bse3DI GCAATG 1 cut(s) 1050
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 164
BseGI GGATG 3 cut(s) 292, 743, 991
BseLI CCNNNNNNNGG 2 cut(s) 46, 1068
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 4 cut(s) 66, 99, 225, 315
BseNI ACTGG 3 cut(s) 492, 739, 1190
BseRI GAGGAG 2 cut(s) 341, 604
BseSI GKGCMC 2 cut(s) 99, 342
BseXI GCAGC 1 cut(s) 1138
BsgI GTGCAG 2 cut(s) 728, 1094
BshFI GGCC 1 cut(s) 181
BshNI GGYRCC 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 1 cut(s) 355
BslFI GGGAC 2 cut(s) 416, 622
BslI CCNNNNNNNGG 2 cut(s) 46, 1068
BsmAI GTCTC 3 cut(s) 129, 526, 1186
BsmFI GGGAC 2 cut(s) 416, 622
BsmI GAATGC 2 cut(s) 448, 1024
BsnI GGCC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 129
Bsp1286I GDGCHC 2 cut(s) 99, 342
Bsp143I GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
BspACI CCGC 1 cut(s) 206
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 4 cut(s) 67, 98, 226, 316
BspLI GGNNCC 5 cut(s) 51, 353, 637, 638, 1060
BspMI ACCTGC 1 cut(s) 884
BspPI GGATC 5 cut(s) 325, 346, 359, 1053, 1066
BspT107I GGYRCC 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 1050
BsrI ACTGG 3 cut(s) 492, 739, 1190
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 1 cut(s) 164
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 107, 1031
Bst6I CTCTTC 4 cut(s) 533, 753, 975, 1189
BstAPI GCANNNNNTGC 1 cut(s) 1049
BstC8I GCNNGC 4 cut(s) 425, 711, 829, 1050
BstDEI CTNAG 6 cut(s) 35, 75, 85, 234, 267, 324
BstF5I GGATG 3 cut(s) 292, 743, 991
BstHHI GCGC 3 cut(s) 74, 363, 955
BstKTI GATC 6 cut(s) 121, 320, 354, 396, 533, 1061
BstMAI GTCTC 3 cut(s) 129, 526, 1186
BstMBI GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
BstMWI GCNNNNNNNGC 1 cut(s) 1049
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 2 cut(s) 831, 1052
BstSCI CCNGG 2 cut(s) 127, 354
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 2 cut(s) 99, 342
BstV1I GCAGC 1 cut(s) 1138
BstX2I RGATCY 3 cut(s) 317, 351, 1058
BstYI RGATCY 3 cut(s) 317, 351, 1058
BsuRI GGCC 1 cut(s) 181
BtsCI GGATG 3 cut(s) 292, 743, 991
BtsI GCAGTG 1 cut(s) 1106
BtsIMutI CAGTG 3 cut(s) 112, 485, 1106
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 4 cut(s) 425, 711, 829, 1050
CfoI GCGC 3 cut(s) 74, 363, 955
Cfr13I GGNCC 1 cut(s) 636
Csp6I GTAC 5 cut(s) 479, 729, 897, 989, 1032
CviAII CATG 4 cut(s) 63, 828, 1049, 1148
CviQI GTAC 5 cut(s) 479, 729, 897, 989, 1032
DdeI CTNAG 6 cut(s) 35, 75, 85, 234, 267, 324
DpnI GATC 6 cut(s) 120, 319, 353, 395, 532, 1060
DpnII GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
Eam1104I CTCTTC 4 cut(s) 533, 753, 975, 1189
EarI CTCTTC 4 cut(s) 533, 753, 975, 1189
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 164
EcoT22I ATGCAT 2 cut(s) 829, 1050
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 4 cut(s) 66, 831, 1052, 1151
FaqI GGGAC 2 cut(s) 416, 622
FatI CATG 4 cut(s) 62, 827, 1048, 1147
Fnu4HI GCNGC 1 cut(s) 1152
FokI GGATG 3 cut(s) 299, 750, 998
Fsp4HI GCNGC 1 cut(s) 1152
FspBI CTAG 3 cut(s) 306, 900, 924
FspI TGCGCA 1 cut(s) 954
GlaI GCGC 3 cut(s) 73, 362, 954
GluI GCNGC 1 cut(s) 1152
HaeIII GGCC 1 cut(s) 181
HapII CCGG 1 cut(s) 355
HhaI GCGC 3 cut(s) 74, 363, 955
Hin1II CATG 4 cut(s) 66, 831, 1052, 1151
Hin6I GCGC 3 cut(s) 72, 361, 953
HinP1I GCGC 3 cut(s) 72, 361, 953
HinfI GANTC 6 cut(s) 6, 509, 880, 908, 935, 999
HpaII CCGG 1 cut(s) 355
HphI GGTGA 4 cut(s) 236, 707, 1136, 1179
Hpy166II GTNNAC 3 cut(s) 340, 479, 1144
Hpy188I TCNGA 5 cut(s) 325, 398, 409, 802, 913
Hpy188III TCNNGA 8 cut(s) 10, 256, 506, 528, 582, 905, 977, 1056
Hpy8I GTNNAC 3 cut(s) 340, 479, 1144
HpyAV CCTTC 4 cut(s) 63, 163, 271, 598
HpyCH4III ACNGT 2 cut(s) 107, 1031
HpyCH4V TGCA 7 cut(s) 340, 446, 709, 827, 932, 1048, 1111
HpyF10VI GCNNNNNNNGC 1 cut(s) 1049
HpyF3I CTNAG 6 cut(s) 35, 75, 85, 234, 267, 324
Hsp92II CATG 4 cut(s) 66, 831, 1052, 1151
HspAI GCGC 3 cut(s) 72, 361, 953
KflI GGGWCCC 1 cut(s) 636
Kzo9I GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
Lsp1109I GCAGC 1 cut(s) 1138
LweI GCATC 5 cut(s) 235, 655, 814, 919, 940
MaeI CTAG 3 cut(s) 306, 900, 924
MalI GATC 6 cut(s) 120, 319, 353, 395, 532, 1060
MboI GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
MboII GAAGA 6 cut(s) 312, 550, 636, 770, 992, 1176
MfeI CAATTG 2 cut(s) 437, 597
MflI RGATCY 3 cut(s) 317, 351, 1058
MhlI GDGCHC 2 cut(s) 99, 342
MluCI AATT 7 cut(s) 292, 365, 437, 597, 862, 886, 962
MlyI GAGTC 1 cut(s) 889
MnlI CCTC 9 cut(s) 40, 198, 229, 319, 492, 582, 747, 976, 984
Mph1103I ATGCAT 2 cut(s) 829, 1050
MroXI GAANNNNTTC 1 cut(s) 403
MseI TTAA 5 cut(s) 111, 723, 764, 1064, 1173
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 355
MspR9I CCNGG 2 cut(s) 129, 356
MunI CAATTG 2 cut(s) 437, 597
Mva1269I GAATGC 2 cut(s) 448, 1024
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 1049
NciI CCSGG 1 cut(s) 356
NdeII GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
NlaIII CATG 4 cut(s) 66, 831, 1052, 1151
NlaIV GGNNCC 5 cut(s) 51, 353, 637, 638, 1060
NsbI TGCGCA 1 cut(s) 954
NsiI ATGCAT 2 cut(s) 829, 1050
NspI RCATGY 2 cut(s) 831, 1052
PaeI GCATGC 2 cut(s) 831, 1052
PceI AGGCCT 1 cut(s) 181
PctI GAATGC 2 cut(s) 448, 1024
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 5 cut(s) 6, 509, 908, 935, 999
PkrI GCNGC 1 cut(s) 1153
PleI GAGTC 1 cut(s) 888
PpsI GAGTC 1 cut(s) 888
PpuMI RGGWCCY 1 cut(s) 636
PshBI ATTAAT 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 5 cut(s) 51, 353, 637, 638, 1060
PspPI GGNCC 1 cut(s) 636
PspPPI RGGWCCY 1 cut(s) 636
PsuI RGATCY 3 cut(s) 317, 351, 1058
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 5 cut(s) 480, 730, 898, 990, 1033
RsaNI GTAC 5 cut(s) 479, 729, 897, 989, 1032
SaqAI TTAA 5 cut(s) 111, 723, 764, 1064, 1173
SatI GCNGC 1 cut(s) 1152
Sau3AI GATC 6 cut(s) 118, 317, 351, 393, 530, 1058
Sau96I GGNCC 1 cut(s) 636
ScaI AGTACT 1 cut(s) 1033
SchI GAGTC 1 cut(s) 889
ScrFI CCNGG 2 cut(s) 129, 356
SduI GDGCHC 2 cut(s) 99, 342
SfaNI GCATC 5 cut(s) 235, 655, 814, 919, 940
SfcI CTRYAG 1 cut(s) 103
SinI GGWCC 1 cut(s) 636
SpeI ACTAGT 1 cut(s) 899
SphI GCATGC 2 cut(s) 831, 1052
Sse9I AATT 7 cut(s) 292, 365, 437, 597, 862, 886, 962
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 1 cut(s) 206
SspMI CTAG 3 cut(s) 306, 900, 924
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 127, 354
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 2 cut(s) 107, 1031
TaqI TCGA 3 cut(s) 243, 527, 878
TaqII GACCGA 1 cut(s) 111
TasI AATT 7 cut(s) 292, 365, 437, 597, 862, 886, 962
TatI WGTACW 1 cut(s) 1031
TfiI GAWTC 5 cut(s) 6, 509, 908, 935, 999
Tru1I TTAA 5 cut(s) 111, 723, 764, 1064, 1173
Tru9I TTAA 5 cut(s) 111, 723, 764, 1064, 1173
TscAI CASTG 3 cut(s) 112, 492, 1113
TseI GCWGC 1 cut(s) 1151
TspDTI ATGAA 2 cut(s) 303, 1152
TspGWI ACGGA 2 cut(s) 958, 1088
TspRI CASTG 3 cut(s) 112, 492, 1113
VneI GTGCAC 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 636
VspI ATTAAT 1 cut(s) 723
XapI RAATTY 1 cut(s) 962
XceI RCATGY 2 cut(s) 831, 1052
XmnI GAANNNNTTC 1 cut(s) 403
XspI CTAG 3 cut(s) 306, 900, 924
ZrmI AGTACT 1 cut(s) 1033
Zsp2I ATGCAT 2 cut(s) 829, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.