AT4G33480

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005107
Physical Location & Seq
Reverse (-)
16105570 .. 16108646
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33480.1

Sequence Viewer

Length: 1011 bp
ATGGCTTTCTCATACTCTTTAACTTCTCCGTCACCAAATTCGACTCTCTCCTATTCCAGTACCCATCAATTTCTCAACCCGTCTTCTTCGTTTCCCGTCTCGTTATCAGTCTCATCTCCGAAGAATACCCGCCATCTTCGTCTCCTCATCACCTCCGCTTTGAATCCACAGACGGGACAGCCGACGAAGAAGGCCTCCACCGGTAGTGATAAGAGTAGTACGAACAAGAAACGTAAGAAAAAGGGGAAAATTGCGAAACCTGTGGAGGATTGGGAATTGAGAGATTCAGAAGACGCTTTTGAGGAAGATGATGATGCCGATTATTCTTCTTCTTCGAGTTCTCTAGCAACTTTTAACTCTCCTCCTACCATACCCAAACCACCTGCTGGGTTTGTGATTAATGAAACTGGTAGGGTTCTTATGGCCTCTAAGAAGCGTATCACCACTGTGATTGATCCAACAAACAATAGCCCTTTGGATTGTGTGATAAGAAGAGTATTTACAAGTAGTAAAGGAGAGGATTGTATGCTTCTTTGCCCTGTGGATACGCCAGTTCAGATTTTGAAGAGCACAAATATTGATGGATGGTCAGCTGTCAGTGATGAAGAGGTCGAATCTCTTCTTCCTGCTGCTGCTTATGCTCTTGCAAAGATCCATATGCATCTAGTACACAGCGGGTTCTGTTACACTGCAAGGGGAGGCTTTTGCTACACGGAAGATAATGTCTTTGATTTTCGCACAGATGATGGCCAAGATGTTGAAGGCTTGCCAACTGAAGGGGTTGAGATTACGTGTTTCCATCTGGATGGATCACACTATATGGTCTACACACCTTCAGATCCACTTCTCTTTGTTGCTGCTAAGGATCAAAATGGGCTGCTGCAAATCGCTGATGATGAACTCTTAGACGATCCAGCTGTGATCAGCGCCATAGACGAGGAAACCGAATTCAATGCCTTGGTGGAGGAAGAGGCGGCTCTCCTGGAATCACTGCTTGGGGAAAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

336

Amino Acids

36.63

Weight (kDa)

4.65

Isoelectric Point (pI)

54.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 219 - 313 1.6e-20 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 386
AccI GTMKAC 1 cut(s) 825
AciI CCGC 4 cut(s) 130, 156, 675, 974
AclWI GGATC 6 cut(s) 449, 646, 817, 833, 873, 905
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 3 cut(s) 37, 947, 1005
AcuI CTGAAG 2 cut(s) 795, 819
AfaI GTAC 3 cut(s) 61, 220, 669
AfiI CCNNNNNNNGG 3 cut(s) 173, 386, 776
AflIII ACRYGT 1 cut(s) 791
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 4 cut(s) 163, 565, 761, 952
AjnI CCWGG 1 cut(s) 981
AjuI GAANNNNNNNTTGG 2 cut(s) 978, 1010
AleI CACNNNNGTG 1 cut(s) 446
AluBI AGCT 2 cut(s) 593, 917
AluI AGCT 2 cut(s) 593, 917
Alw21I GWGCWC 1 cut(s) 572
Alw26I GTCTC 3 cut(s) 103, 115, 146
AlwI GGATC 6 cut(s) 449, 646, 817, 833, 873, 905
AoxI GGCC 3 cut(s) 192, 423, 748
ApeKI GCWGC 5 cut(s) 629, 632, 857, 877, 880
ApoI RAATTY 3 cut(s) 37, 947, 1005
AseI ATTAAT 1 cut(s) 399
AsiGI ACCGGT 1 cut(s) 200
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 1 cut(s) 929
AsuHPI GGTGA 3 cut(s) 24, 142, 433
BaeI ACNNNNGTAYC 2 cut(s) 537, 570
BalI TGGCCA 1 cut(s) 750
BarI GAAGNNNNNNTAC 2 cut(s) 484, 516
BbsI GAAGAC 2 cut(s) 75, 297
Bbv12I GWGCWC 1 cut(s) 572
BbvI GCAGC 5 cut(s) 616, 619, 844, 864, 867
BccI CCATC 7 cut(s) 72, 141, 575, 579, 740, 800, 807
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 1 cut(s) 983
BciVI GTATCC 1 cut(s) 538
BclI TGATCA 1 cut(s) 921
BcoDI GTCTC 3 cut(s) 103, 115, 146
BfaI CTAG 2 cut(s) 344, 665
BfoI RGCGCY 1 cut(s) 930
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 538
BisI GCNGC 6 cut(s) 630, 633, 858, 878, 881, 975
BlsI GCNGC 6 cut(s) 631, 634, 859, 879, 882, 976
Bme1390I CCNGG 1 cut(s) 983
BmrFI CCNGG 1 cut(s) 983
BmsI GCATC 2 cut(s) 304, 670
BpiI GAAGAC 2 cut(s) 75, 297
Bpu10I CCTNAGC 1 cut(s) 861
BsaAI YACGTR 1 cut(s) 792
BsaJI CCNNGG 1 cut(s) 957
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 386, 776
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 3 cut(s) 57, 412, 551
BseBI CCWGG 1 cut(s) 983
BseDI CCNNGG 1 cut(s) 957
BseGI GGATG 2 cut(s) 590, 811
BseLI CCNNNNNNNGG 3 cut(s) 173, 386, 776
BseNI ACTGG 3 cut(s) 57, 412, 551
BseRI GAGGAG 2 cut(s) 134, 351
BseXI GCAGC 5 cut(s) 616, 619, 844, 864, 867
BseYI CCCAGC 1 cut(s) 386
BshFI GGCC 3 cut(s) 194, 425, 750
BshTI ACCGGT 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 572
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 3 cut(s) 173, 386, 776
BsmAI GTCTC 3 cut(s) 103, 115, 146
BsmBI CGTCTC 2 cut(s) 103, 146
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 3 cut(s) 194, 425, 750
Bsp1286I GDGCHC 1 cut(s) 572
Bsp143I GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
BspACI CCGC 4 cut(s) 130, 156, 675, 974
BspANI GGCC 3 cut(s) 194, 425, 750
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 6 cut(s) 449, 646, 817, 833, 873, 905
BspQI GCTCTTC 1 cut(s) 560
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 3 cut(s) 57, 412, 551
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 957
BssMI GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
BssT1I CCWWGG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 983
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 5 cut(s) 487, 560, 600, 624, 963
BstBAI YACGTR 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 767
BstDEI CTNAG 3 cut(s) 429, 861, 904
BstF5I GGATG 2 cut(s) 590, 811
BstH2I RGCGCY 1 cut(s) 930
BstHHI GCGC 1 cut(s) 929
BstKTI GATC 7 cut(s) 457, 654, 812, 841, 868, 913, 924
BstMAI GTCTC 3 cut(s) 103, 115, 146
BstMBI GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNI CCWGG 1 cut(s) 983
BstSCI CCNGG 1 cut(s) 981
BstV1I GCAGC 5 cut(s) 616, 619, 844, 864, 867
BstV2I GAAGAC 2 cut(s) 75, 297
BstX2I RGATCY 2 cut(s) 651, 838
BstXI CCANNNNNNTGG 1 cut(s) 806
BstYI RGATCY 2 cut(s) 651, 838
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 3 cut(s) 194, 425, 750
BtsCI GGATG 2 cut(s) 590, 811
BtsI GCAGTG 2 cut(s) 687, 989
BtsIMutI CAGTG 4 cut(s) 444, 604, 687, 989
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 767
CfoI GCGC 1 cut(s) 929
Cfr10I RCCGGY 1 cut(s) 200
CseI GACGC 1 cut(s) 302
Csp6I GTAC 3 cut(s) 60, 219, 668
CspAI ACCGGT 1 cut(s) 200
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviQI GTAC 3 cut(s) 60, 219, 668
DdeI CTNAG 3 cut(s) 429, 861, 904
DpnI GATC 7 cut(s) 456, 653, 811, 840, 867, 912, 923
DpnII GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 5 cut(s) 487, 560, 600, 624, 963
EarI CTCTTC 5 cut(s) 487, 560, 600, 624, 963
Eco130I CCWWGG 1 cut(s) 957
Eco147I AGGCCT 1 cut(s) 194
Eco57I CTGAAG 2 cut(s) 795, 819
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 1 cut(s) 981
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 957
Esp3I CGTCTC 2 cut(s) 103, 146
FaqI GGGAC 1 cut(s) 189
FauI CCCGC 2 cut(s) 137, 668
FauNDI CATATG 1 cut(s) 657
FbaI TGATCA 1 cut(s) 921
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 6 cut(s) 630, 633, 858, 878, 881, 975
FokI GGATG 2 cut(s) 597, 818
Fsp4HI GCNGC 6 cut(s) 630, 633, 858, 878, 881, 975
FspBI CTAG 2 cut(s) 344, 665
GlaI GCGC 1 cut(s) 928
GluI GCNGC 6 cut(s) 630, 633, 858, 878, 881, 975
GsaI CCCAGC 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 930
HaeIII GGCC 3 cut(s) 194, 425, 750
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 929
Hin6I GCGC 1 cut(s) 927
HinP1I GCGC 1 cut(s) 927
HinfI GANTC 5 cut(s) 43, 163, 284, 614, 986
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 24, 142, 433
Hpy166II GTNNAC 2 cut(s) 670, 826
Hpy188I TCNGA 4 cut(s) 120, 289, 558, 838
Hpy188III TCNNGA 1 cut(s) 803
Hpy8I GTNNAC 2 cut(s) 670, 826
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 184, 755, 770, 843
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 2 cut(s) 232, 791
HpyCH4V TGCA 4 cut(s) 647, 661, 692, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
HpyF3I CTNAG 3 cut(s) 429, 861, 904
HpySE526I ACGT 2 cut(s) 232, 791
HspAI GCGC 1 cut(s) 927
Ksp22I TGATCA 1 cut(s) 921
Kzo9I GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
LguI GCTCTTC 1 cut(s) 560
Lsp1109I GCAGC 5 cut(s) 616, 619, 844, 864, 867
LweI GCATC 2 cut(s) 304, 670
MaeI CTAG 2 cut(s) 344, 665
MaeII ACGT 2 cut(s) 232, 791
MaeIII GTNAC 2 cut(s) 30, 683
MalI GATC 7 cut(s) 456, 653, 811, 840, 867, 912, 923
MboI GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
MflI RGATCY 2 cut(s) 651, 838
MhlI GDGCHC 1 cut(s) 572
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 6 cut(s) 37, 68, 249, 275, 947, 1005
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 1 cut(s) 37
MmeI TCCRAC 1 cut(s) 482
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 1 cut(s) 663
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 750
MseI TTAA 3 cut(s) 20, 354, 399
MslI CAYNNNNRTG 2 cut(s) 446, 804
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 3 cut(s) 593, 675, 917
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 983
MvaI CCWGG 1 cut(s) 983
MwoI GCNNNNNNNGC 1 cut(s) 638
NdeI CATATG 1 cut(s) 657
NdeII GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
NmuCI GTSAC 1 cut(s) 30
NsiI ATGCAT 1 cut(s) 663
OliI CACNNNNGTG 1 cut(s) 446
PaqCI CACCTGC 1 cut(s) 391
PceI AGGCCT 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 942
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 4 cut(s) 163, 284, 614, 986
PflMI CCANNNNNTGG 1 cut(s) 386
PfoI TCCNGGA 1 cut(s) 981
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 6 cut(s) 631, 634, 859, 879, 882, 976
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
Ppu21I YACGTR 1 cut(s) 792
PshBI ATTAAT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 981
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 981
PsuI RGATCY 2 cut(s) 651, 838
PvuII CAGCTG 2 cut(s) 593, 917
RsaI GTAC 3 cut(s) 61, 220, 669
RsaNI GTAC 3 cut(s) 60, 219, 668
RseI CAYNNNNRTG 2 cut(s) 446, 804
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 3 cut(s) 20, 354, 399
SatI GCNGC 6 cut(s) 630, 633, 858, 878, 881, 975
Sau3AI GATC 7 cut(s) 454, 651, 809, 838, 865, 910, 921
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 1 cut(s) 983
SduI GDGCHC 1 cut(s) 572
SetI ASST 9 cut(s) 155, 235, 262, 385, 595, 612, 794, 835, 919
SfaNI GCATC 2 cut(s) 304, 670
SmiMI CAYNNNNRTG 2 cut(s) 446, 804
Sse9I AATT 6 cut(s) 37, 68, 249, 275, 947, 1005
SseBI AGGCCT 1 cut(s) 194
SsiI CCGC 4 cut(s) 130, 156, 675, 974
SspI AATATT 1 cut(s) 577
SspMI CTAG 2 cut(s) 344, 665
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 981
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 2 cut(s) 235, 794
TaqI TCGA 3 cut(s) 41, 335, 612
TasI AATT 6 cut(s) 37, 68, 249, 275, 947, 1005
TatI WGTACW 1 cut(s) 667
TauI GCSGC 1 cut(s) 977
TfiI GAWTC 4 cut(s) 163, 284, 614, 986
Tru1I TTAA 3 cut(s) 20, 354, 399
Tru9I TTAA 3 cut(s) 20, 354, 399
TscAI CASTG 4 cut(s) 451, 604, 694, 996
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 5 cut(s) 629, 632, 857, 877, 880
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 3 cut(s) 417, 618, 912
TspGWI ACGGA 2 cut(s) 18, 728
TspRI CASTG 4 cut(s) 451, 604, 694, 996
Van91I CCANNNNNTGG 1 cut(s) 386
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 3 cut(s) 37, 947, 1005
XmiI GTMKAC 1 cut(s) 825
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 2 cut(s) 344, 665
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.