MD00G1064700.v1.1

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005107
Physical Location & Seq
Reverse (-)
12268171 .. 12283479
15309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1064700.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGCATTCTCCTCACTCTCACTGTCCACAACTCCATCAACCATCACCTGCAGTCTCCATTCCAAGCGACCCCACCACCTCCCCCTGCTCGCTTTCCACTCCAACCAGCATCTATTCCCACTCTCAATCTCGCTCCCATCCCCTCCTTCATCGACCCCCGCCAGACTCCGTATCTCTCTCTGCGCTGCCGCAAAGTCACAGACTGGCCCCGTCAAGAAGCGCTCACCTTCGCCCACCAACGACAACAAGAAGAAAAAGCGCAAGGGTGGCAGTGGCGGTGGCAATTCCGAGGATCTGAGCTTCGGTGATGTTGAAATTATGGATAGTTCTGGTGGTGGGGTTTCAAATTCGAGGTCATTGGGTTTCCATCCAACACGGCTGCCAAATCCACCGGCTGGGTTTGTGGTGGATGATCATGGGAAGGTGCTCATGGCTTCTTCCAAGCGAATTGCTTCCATTGTCGATCCTACCAATAATTTCCCTCTAGAGTGTGTTATAAGGAGAGTATTCAGAAGCTCGCGAGGGGATGAATGTATGCTGCTTTGCCCAGTTGACACGCCCGTTCAAATATTAAAGAGCACAAACATAGATGGCTGGTCAGCTGTCAGTGATGAAGAAGTTGAAGCTATTCTTCCCTCTGCTGCTTATGCCCTTGCTAAGATACACATGCATCTAGTACATAGTGGATTCTGCTATACAGCACGGGGAGGGTTTTGTTACTCCGAAGATGACATATTTGATTTCGGTACAGATGATGGTCAAGATGTAGATGGCCTGCCAACCGAGGGTGTAGAAATTACATGTTTCCATCTGGATGGTTCACATTACATGATTTATACGCCGTCTGACCCACTTCTCTTTGTTGTTGTAAAGGATAATGCTGGTTTGTTGCAAATTGCTGACGATGACATGCTGGAGGATCCTACTATCATAAGTGCCATAGATGAGGAGACTGAGTTTAACGCCTTGGTGGAGGAAGAAGCAGCTCTTCTCGATTCATTGCTAGGAAAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

339

Amino Acids

36.39

Weight (kDa)

5.06

Isoelectric Point (pI)

59.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 222 - 316 2.3e-19 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AarI CACCTGC 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 395
AccII CGCG 1 cut(s) 520
AciI CCGC 3 cut(s) 159, 189, 276
AclWI GGATC 4 cut(s) 300, 458, 914, 927
AcsI RAATTY 1 cut(s) 346
AfaI GTAC 2 cut(s) 678, 748
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 395, 785
AflIII ACRYGT 1 cut(s) 800
AgsI TTSAA 4 cut(s) 314, 345, 566, 623
AluBI AGCT 5 cut(s) 300, 516, 602, 626, 986
AluI AGCT 5 cut(s) 300, 516, 602, 626, 986
Alw21I GWGCWC 2 cut(s) 429, 581
Alw26I GTCTC 2 cut(s) 59, 944
AlwI GGATC 4 cut(s) 300, 458, 914, 927
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 2 cut(s) 205, 772
ApeKI GCWGC 5 cut(s) 185, 379, 538, 641, 983
ApoI RAATTY 1 cut(s) 346
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
Asp700I GAANNNNTTC 2 cut(s) 451, 627
AspLEI GCGC 3 cut(s) 185, 222, 261
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 37, 216, 317
BamHI GGATCC 1 cut(s) 919
Bbv12I GWGCWC 2 cut(s) 429, 581
BbvI GCAGC 5 cut(s) 172, 366, 525, 628, 995
BccI CCATC 9 cut(s) 43, 50, 145, 375, 584, 749, 764, 809, 816
BceAI ACGGC 2 cut(s) 392, 826
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 2 cut(s) 59, 944
BfaI CTAG 3 cut(s) 485, 674, 1004
BfmI CTRYAG 1 cut(s) 49
BfoI RGCGCY 1 cut(s) 223
BfuAI ACCTGC 1 cut(s) 56
BisI GCNGC 6 cut(s) 186, 189, 380, 539, 642, 984
BlsI GCNGC 6 cut(s) 187, 190, 381, 540, 643, 985
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 208, 921
BmrI ACTGGG 1 cut(s) 542
BmsI GCATC 2 cut(s) 118, 679
BmuI ACTGGG 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 935
BsaJI CCNNGG 3 cut(s) 288, 783, 966
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 2 cut(s) 395, 785
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 2 cut(s) 208, 548
Bse3DI GCAATG 1 cut(s) 998
BseDI CCNNGG 3 cut(s) 288, 783, 966
BseGI GGATG 5 cut(s) 137, 367, 415, 532, 820
BseLI CCNNNNNNNGG 2 cut(s) 395, 785
BseMI GCAATG 1 cut(s) 998
BseMII CTCAG 2 cut(s) 287, 945
BseNI ACTGG 2 cut(s) 208, 548
BseRI GAGGAG 1 cut(s) 962
BseXI GCAGC 5 cut(s) 172, 366, 525, 628, 995
BseYI CCCAGC 1 cut(s) 395
Bsh1236I CGCG 1 cut(s) 520
BshFI GGCC 2 cut(s) 207, 774
BsiHKAI GWGCWC 2 cut(s) 429, 581
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 395, 785
BsmAI GTCTC 2 cut(s) 59, 944
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 2 cut(s) 207, 774
Bsp1286I GDGCHC 2 cut(s) 429, 581
Bsp143I GATC 4 cut(s) 292, 412, 463, 919
Bsp68I TCGCGA 1 cut(s) 520
BspACI CCGC 3 cut(s) 159, 189, 276
BspANI GGCC 2 cut(s) 207, 774
BspCNI CTCAG 2 cut(s) 288, 946
BspFNI CGCG 1 cut(s) 520
BspLI GGNNCC 2 cut(s) 208, 921
BspMAI CTGCAG 1 cut(s) 53
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 4 cut(s) 300, 458, 914, 927
BspQI GCTCTTC 1 cut(s) 993
BsrDI GCAATG 1 cut(s) 998
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 2 cut(s) 208, 548
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 288, 783, 966
BssMI GATC 4 cut(s) 292, 412, 463, 919
BssT1I CCWWGG 1 cut(s) 966
Bst4CI ACNGT 1 cut(s) 24
Bst6I CTCTTC 1 cut(s) 993
BstC8I GCNNGC 3 cut(s) 90, 518, 776
BstDEI CTNAG 3 cut(s) 296, 657, 954
BstF5I GGATG 5 cut(s) 137, 367, 415, 532, 820
BstFNI CGCG 1 cut(s) 520
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 3 cut(s) 185, 222, 261
BstKTI GATC 4 cut(s) 295, 415, 466, 922
BstMAI GTCTC 2 cut(s) 59, 944
BstMBI GATC 4 cut(s) 292, 412, 463, 919
BstMWI GCNNNNNNNGC 2 cut(s) 267, 647
BstNSI RCATGY 3 cut(s) 670, 804, 913
BstSFI CTRYAG 1 cut(s) 49
BstUI CGCG 1 cut(s) 520
BstV1I GCAGC 5 cut(s) 172, 366, 525, 628, 995
BstX2I RGATCY 2 cut(s) 292, 919
BstXI CCANNNNNNTGG 1 cut(s) 815
BstYI RGATCY 2 cut(s) 292, 919
BsuRI GGCC 2 cut(s) 207, 774
BtsCI GGATG 5 cut(s) 137, 367, 415, 532, 820
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 3 cut(s) 20, 277, 613
BtuMI TCGCGA 1 cut(s) 520
BveI ACCTGC 1 cut(s) 56
Cac8I GCNNGC 3 cut(s) 90, 518, 776
CfoI GCGC 3 cut(s) 185, 222, 261
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 677, 747
CviAII CATG 6 cut(s) 416, 430, 667, 801, 829, 910
CviQI GTAC 2 cut(s) 677, 747
DdeI CTNAG 3 cut(s) 296, 657, 954
DpnI GATC 4 cut(s) 294, 414, 465, 921
DpnII GATC 4 cut(s) 292, 412, 463, 919
Eam1104I CTCTTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 993
Eco130I CCWWGG 1 cut(s) 966
Eco47III AGCGCT 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 966
EcoT22I ATGCAT 1 cut(s) 672
ErhI CCWWGG 1 cut(s) 966
FaeI CATG 6 cut(s) 419, 433, 670, 804, 832, 913
FalI AAGNNNNNCTT 4 cut(s) 615, 647, 972, 1004
FatI CATG 6 cut(s) 415, 429, 666, 800, 828, 909
FauI CCCGC 1 cut(s) 166
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 6 cut(s) 186, 189, 380, 539, 642, 984
FokI GGATG 5 cut(s) 124, 354, 422, 539, 827
Fsp4HI GCNGC 6 cut(s) 186, 189, 380, 539, 642, 984
FspBI CTAG 3 cut(s) 485, 674, 1004
GlaI GCGC 3 cut(s) 184, 221, 260
GluI GCNGC 6 cut(s) 186, 189, 380, 539, 642, 984
GsaI CCCAGC 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 2 cut(s) 207, 774
HapII CCGG 1 cut(s) 392
HhaI GCGC 3 cut(s) 185, 222, 261
Hin1II CATG 6 cut(s) 419, 433, 670, 804, 832, 913
Hin6I GCGC 3 cut(s) 183, 220, 259
HinP1I GCGC 3 cut(s) 183, 220, 259
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 3 cut(s) 165, 687, 995
HpaII CCGG 1 cut(s) 392
HphI GGTGA 3 cut(s) 37, 216, 317
Hpy166II GTNNAC 3 cut(s) 27, 553, 821
Hpy188I TCNGA 5 cut(s) 289, 297, 512, 724, 847
Hpy188III TCNNGA 6 cut(s) 214, 485, 519, 761, 812, 992
Hpy8I GTNNAC 3 cut(s) 27, 553, 821
HpyAV CCTTC 3 cut(s) 156, 237, 415
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4V TGCA 3 cut(s) 51, 670, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 647
HpyF3I CTNAG 3 cut(s) 296, 657, 954
Hsp92II CATG 6 cut(s) 419, 433, 670, 804, 832, 913
HspAI GCGC 3 cut(s) 183, 220, 259
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 4 cut(s) 292, 412, 463, 919
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 5 cut(s) 172, 366, 525, 628, 995
LweI GCATC 2 cut(s) 118, 679
MaeI CTAG 3 cut(s) 485, 674, 1004
MaeIII GTNAC 2 cut(s) 195, 716
MalI GATC 4 cut(s) 294, 414, 465, 921
MboI GATC 4 cut(s) 292, 412, 463, 919
MboII GAAGA 7 cut(s) 262, 429, 623, 626, 737, 980, 989
MflI RGATCY 2 cut(s) 292, 919
MhlI GDGCHC 2 cut(s) 429, 581
MluCI AATT 7 cut(s) 283, 315, 346, 447, 475, 795, 894
MlyI GAGTC 1 cut(s) 159
MmeI TCCRAC 2 cut(s) 126, 395
Mph1103I ATGCAT 1 cut(s) 672
MroXI GAANNNNTTC 2 cut(s) 451, 627
MseI TTAA 2 cut(s) 572, 960
MslI CAYNNNNRTG 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 602
MspI CCGG 1 cut(s) 392
Mva1269I GAATGC 1 cut(s) 5
MvnI CGCG 1 cut(s) 520
MwoI GCNNNNNNNGC 2 cut(s) 267, 647
NdeII GATC 4 cut(s) 292, 412, 463, 919
NlaIII CATG 6 cut(s) 419, 433, 670, 804, 832, 913
NlaIV GGNNCC 2 cut(s) 208, 921
NmuCI GTSAC 1 cut(s) 195
NruI TCGCGA 1 cut(s) 520
NsiI ATGCAT 1 cut(s) 672
NspI RCATGY 3 cut(s) 670, 804, 913
PaqCI CACCTGC 1 cut(s) 56
PciI ACATGT 1 cut(s) 800
PciSI GCTCTTC 1 cut(s) 993
PctI GAATGC 1 cut(s) 5
PdmI GAANNNNTTC 2 cut(s) 451, 627
PfeI GAWTC 2 cut(s) 687, 995
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 6 cut(s) 187, 190, 381, 540, 643, 985
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
PscI ACATGT 1 cut(s) 800
PsiI TTATAA 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 395
PspN4I GGNNCC 2 cut(s) 208, 921
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 1 cut(s) 53
PsuI RGATCY 2 cut(s) 292, 919
PvuII CAGCTG 1 cut(s) 602
RruI TCGCGA 1 cut(s) 520
RsaI GTAC 2 cut(s) 678, 748
RsaNI GTAC 2 cut(s) 677, 747
RseI CAYNNNNRTG 1 cut(s) 813
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 2 cut(s) 572, 960
SatI GCNGC 6 cut(s) 186, 189, 380, 539, 642, 984
Sau3AI GATC 4 cut(s) 292, 412, 463, 919
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 159
SduI GDGCHC 2 cut(s) 429, 581
SfaNI GCATC 2 cut(s) 118, 679
SfcI CTRYAG 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 813
Sse9I AATT 7 cut(s) 283, 315, 346, 447, 475, 795, 894
SsiI CCGC 3 cut(s) 159, 189, 276
SspI AATATT 1 cut(s) 570
SspMI CTAG 3 cut(s) 485, 674, 1004
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 1 cut(s) 24
TaqI TCGA 4 cut(s) 152, 350, 462, 993
TasI AATT 7 cut(s) 283, 315, 346, 447, 475, 795, 894
TatI WGTACW 1 cut(s) 676
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 2 cut(s) 687, 995
Tru1I TTAA 2 cut(s) 572, 960
Tru9I TTAA 2 cut(s) 572, 960
TscAI CASTG 3 cut(s) 27, 277, 613
TseFI GTSAC 1 cut(s) 195
TseI GCWGC 5 cut(s) 185, 379, 538, 641, 983
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 4 cut(s) 138, 543, 627, 987
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 3 cut(s) 27, 277, 613
Van91I CCANNNNNTGG 1 cut(s) 395
XapI RAATTY 1 cut(s) 346
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 3 cut(s) 670, 804, 913
XmnI GAANNNNTTC 2 cut(s) 451, 627
XspI CTAG 3 cut(s) 485, 674, 1004
Zsp2I ATGCAT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.