Rroxscaffold_2G00121230

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005107
Physical Location & Seq
Forward (+)
53743622 .. 53749908
6287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121230.1

Sequence Viewer

Length: 1428 bp
ATGCAGACTATGGCTTTCTCAGTCTCCACAGCCACAGCCACAGCCACAGCCAGTTCCTATCCCCAATTCCATTTCTTCTCTTTTCACTCCAATCACCTATTCCCCGTCTCCCTCTCTCTCCCAACCCACACCGCCAGACTCCTCGTCTCCTGCTGCGCCGCCGCAAAGCCTCAGACCGGCCCCGTCAAGAAGAAGCGCTCTCCTCCCTCGCCCACCACCACCGCCGCCGCCAACAACAACGGAAACAACAACAAGAAAAAGCGTAAGGCGAAGGATAATAATACCAGCTTTAGCAAGGTGGAAATTGTGGATGATTTCGGGTCTTCAGATTCCGGGTCGCTGGGTTACCATCCTACGCCGCTGCCGAAGCCGCCCGCTGGGTTTGTAGTGGATGATCACGGCAGGGTGCTTATGGCTTCGTCCAAGCGAATAGCCTCGATTATTGTGGTGAAAGCTGGGTTTTCTGATTTGGTTGCGAGAACGGAGAAAAAGGAAGGGAATGAGAATTGTAAGAGTCCATTTCGCTGGGAACTCATGCTGAATATTATATTGAGTATGCTGCCTGGAGGGAGTGGGAAATCAGATACTGATAAGGAGTCTTTGTTTTTAGAACACACTGATGAGAACAGGCTTTTGGAGGATAGAGAGCCGTTTCTTGAGGCAACAAGGGATTTTAGTAGGAATGCGCTGGAGATTAACCATGACGGGAGAGCTGAGGCCCTAAACTCAAGCAAGAGGACTAGAAGGAGAATGATTATTTTTTGGACTGTGGTGGACCAGTGGCAGGACTGTGGTGGACTGGTGATATGTGAAAGGCAGGGCTCTCGGAAACCTGGAAGCCAAAGGAAACAAGTTGAGTTCTTTAAAGAAACGATAGAGAGTACCTTGTCTCCAGCAATGAAGAGGAGGCAGCAAAGATTACAGGATTTTTTTGGAAAGAAGGTGTTGGTTTTGGGGAGATCTGCTATGAAGGCAGTGTTGGTCAGTGATGAAGAAGTTGAAGCTATTCTTCCTTCTGCTGCTTATGCCCTTGCCAAGATACATATGCATCTTGTACATAGCGGGTATGATGCCACATATTCACAAGCCTTATTATTCTCAAATCCAGTGGCTATGATATCTGTATTTGATATGTGCCTTTCTATTTTATACTCTTCCTTTCTATTATTTATTTATGGTGCTGACTATTCTGCAGATGATGGTGAAGATGTAGATGGCTTGCCAACTGAGGGTGTCGAGATTACATGTTTCCATTTGGATGGTGCGCATTACATGGTTTACACACCCTCTGATCCACTTCTCTTTGTTGCTGTAAAGGATCTGCTGGAGGACCCTGCCATCATTAGTGCCATAGATGAGGAGACCGAGTTTAACGCATTGGTGGAGGAGGAAGCAGCTCTTCTTGATTCATTGCTAGGAAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

475

Amino Acids

52.43

Weight (kDa)

5.86

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 400 - 463 1.1e-08 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1266
AclWI GGATC 2 cut(s) 1286, 1326
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 883, 1056
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 4 cut(s) 176, 377, 784, 1229
AflIII ACRYGT 1 cut(s) 1244
AgsI TTSAA 1 cut(s) 1001
AhdI GACNNNNNGTC 1 cut(s) 143
AjnI CCWGG 2 cut(s) 562, 832
AjuI GAANNNNNNNTTGG 6 cut(s) 83, 115, 617, 649, 962, 994
AluBI AGCT 5 cut(s) 288, 455, 713, 1004, 1397
AluI AGCT 5 cut(s) 288, 455, 713, 1004, 1397
Alw26I GTCTC 5 cut(s) 28, 112, 151, 894, 1355
AlwI GGATC 2 cut(s) 1286, 1326
AlwNI CAGNNNCTG 1 cut(s) 587
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 2 cut(s) 178, 717
ApeKI GCWGC 6 cut(s) 153, 361, 559, 910, 1019, 1394
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 4 cut(s) 158, 198, 688, 1267
AspS9I GGNCC 4 cut(s) 179, 718, 775, 1330
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 4 cut(s) 86, 460, 814, 1214
AvaII GGWCC 2 cut(s) 775, 1330
BanII GRGCYC 1 cut(s) 824
BbsI GAAGAC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 6 cut(s) 140, 348, 546, 922, 1006, 1406
BccI CCATC 5 cut(s) 357, 1193, 1208, 1253, 1346
BceAI ACGGC 2 cut(s) 415, 634
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 2 cut(s) 564, 834
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 5 cut(s) 28, 112, 151, 894, 1355
BfaI CTAG 2 cut(s) 741, 1415
BfmI CTRYAG 1 cut(s) 1191
BfoI RGCGCY 1 cut(s) 199
BglII AGATCT 1 cut(s) 959
Bme1390I CCNGG 3 cut(s) 334, 564, 834
Bme18I GGWCC 2 cut(s) 775, 1330
BmeRI GACNNNNNGTC 1 cut(s) 143
BmgT120I GGNCC 4 cut(s) 179, 718, 775, 1330
BmiI GGNNCC 2 cut(s) 181, 1332
BmrFI CCNGG 3 cut(s) 334, 564, 834
BmsI GCATC 2 cut(s) 1057, 1060
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 4 cut(s) 585, 710, 876, 1346
Bpu10I CCTNAGC 1 cut(s) 714
BpuEI CTTGAG 2 cut(s) 677, 712
BpuMI CCSGG 1 cut(s) 334
BsaI GGTCTC 1 cut(s) 1355
BsaXI ACNNNNNCTCC 6 cut(s) 587, 617, 874, 904, 1352, 1382
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 377, 784, 1229
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 4 cut(s) 51, 778, 804, 1106
Bse3DI GCAATG 2 cut(s) 903, 1409
BseBI CCWGG 2 cut(s) 564, 834
BseGI GGATG 4 cut(s) 316, 349, 397, 1264
BseLI CCNNNNNNNGG 4 cut(s) 176, 377, 784, 1229
BseMI GCAATG 2 cut(s) 903, 1409
BseMII CTCAG 4 cut(s) 33, 185, 705, 1218
BseNI ACTGG 4 cut(s) 51, 778, 804, 1106
BseRI GAGGAG 5 cut(s) 131, 192, 919, 1373, 1400
BseXI GCAGC 6 cut(s) 140, 348, 546, 922, 1006, 1406
BseYI CCCAGC 4 cut(s) 340, 377, 455, 525
BshFI GGCC 2 cut(s) 180, 719
BsiSI CCGG 2 cut(s) 177, 333
BslI CCNNNNNNNGG 4 cut(s) 176, 377, 784, 1229
BsmAI GTCTC 5 cut(s) 28, 112, 151, 894, 1355
BsmBI CGTCTC 2 cut(s) 112, 151
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 2 cut(s) 180, 719
Bso31I GGTCTC 1 cut(s) 1355
Bsp1286I GDGCHC 1 cut(s) 824
Bsp1407I TGTACA 1 cut(s) 1054
Bsp143I GATC 4 cut(s) 394, 959, 1291, 1318
BspANI GGCC 2 cut(s) 180, 719
BspCNI CTCAG 4 cut(s) 32, 184, 706, 1219
BspLI GGNNCC 2 cut(s) 181, 1332
BspMAI CTGCAG 1 cut(s) 1195
BspPI GGATC 2 cut(s) 1286, 1326
BspQI GCTCTTC 1 cut(s) 1404
BspTNI GGTCTC 1 cut(s) 1355
BsrDI GCAATG 2 cut(s) 903, 1409
BsrFI RCCGGY 1 cut(s) 176
BsrGI TGTACA 1 cut(s) 1054
BsrI ACTGG 4 cut(s) 51, 778, 804, 1106
BssAI RCCGGY 1 cut(s) 176
BssMI GATC 4 cut(s) 394, 959, 1291, 1318
Bst2UI CCWGG 2 cut(s) 564, 834
Bst4CI ACNGT 2 cut(s) 769, 791
Bst6I CTCTTC 3 cut(s) 896, 1159, 1404
BstAUI TGTACA 1 cut(s) 1054
BstC8I GCNNGC 2 cut(s) 375, 1220
BstDEI CTNAG 4 cut(s) 19, 171, 714, 1227
BstEII GGTNACC 1 cut(s) 344
BstF5I GGATG 4 cut(s) 316, 349, 397, 1264
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 4 cut(s) 158, 198, 688, 1267
BstKTI GATC 4 cut(s) 397, 962, 1294, 1321
BstMAI GTCTC 5 cut(s) 28, 112, 151, 894, 1355
BstMBI GATC 4 cut(s) 394, 959, 1291, 1318
BstMWI GCNNNNNNNGC 4 cut(s) 367, 370, 971, 1025
BstNI CCWGG 2 cut(s) 564, 834
BstNSI RCATGY 1 cut(s) 1248
BstPI GGTNACC 1 cut(s) 344
BstSCI CCNGG 3 cut(s) 332, 562, 832
BstSFI CTRYAG 1 cut(s) 1191
BstV1I GCAGC 6 cut(s) 140, 348, 546, 922, 1006, 1406
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 2 cut(s) 959, 1318
BstXI CCANNNNNNTGG 2 cut(s) 525, 1259
BstYI RGATCY 2 cut(s) 959, 1318
BsuRI GGCC 2 cut(s) 180, 719
BtsCI GGATG 4 cut(s) 316, 349, 397, 1264
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 5 cut(s) 615, 785, 981, 991, 1113
Cac8I GCNNGC 2 cut(s) 375, 1220
CaiI CAGNNNCTG 1 cut(s) 587
CfoI GCGC 4 cut(s) 158, 198, 688, 1267
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 4 cut(s) 179, 718, 775, 1330
Csp6I GTAC 2 cut(s) 882, 1055
CspCI CAANNNNNGTGG 2 cut(s) 1089, 1124
CviAII CATG 4 cut(s) 535, 701, 1245, 1273
CviQI GTAC 2 cut(s) 882, 1055
DdeI CTNAG 4 cut(s) 19, 171, 714, 1227
DpnI GATC 4 cut(s) 396, 961, 1293, 1320
DpnII GATC 4 cut(s) 394, 959, 1291, 1318
DraI TTTAAA 1 cut(s) 865
DriI GACNNNNNGTC 1 cut(s) 143
Eam1104I CTCTTC 3 cut(s) 896, 1159, 1404
Eam1105I GACNNNNNGTC 1 cut(s) 143
EarI CTCTTC 3 cut(s) 896, 1159, 1404
Eco24I GRGCYC 1 cut(s) 824
Eco31I GGTCTC 1 cut(s) 1355
Eco32I GATATC 1 cut(s) 1119
Eco47I GGWCC 2 cut(s) 775, 1330
Eco47III AGCGCT 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 344
EcoO109I RGGNCCY 2 cut(s) 718, 1330
EcoO65I GGTNACC 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 562, 832
EcoRV GATATC 1 cut(s) 1119
EcoT22I ATGCAT 1 cut(s) 1050
EcoT38I GRGCYC 1 cut(s) 824
Esp3I CGTCTC 2 cut(s) 112, 151
FaeI CATG 4 cut(s) 538, 704, 1248, 1276
FalI AAGNNNNNCTT 4 cut(s) 993, 1025, 1383, 1415
FatI CATG 4 cut(s) 534, 700, 1244, 1272
FauI CCCGC 2 cut(s) 382, 1055
FauNDI CATATG 1 cut(s) 1044
FbaI TGATCA 1 cut(s) 394
FokI GGATG 4 cut(s) 323, 336, 404, 1271
FriOI GRGCYC 1 cut(s) 824
FspAI RTGCGCAY 1 cut(s) 1266
FspBI CTAG 2 cut(s) 741, 1415
FspI TGCGCA 1 cut(s) 1266
GlaI GCGC 4 cut(s) 157, 197, 687, 1266
GsaI CCCAGC 4 cut(s) 344, 381, 459, 529
GsuI CTGGAG 4 cut(s) 585, 710, 876, 1346
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 180, 719
HapII CCGG 2 cut(s) 177, 333
HhaI GCGC 4 cut(s) 158, 198, 688, 1267
Hin1II CATG 4 cut(s) 538, 704, 1248, 1276
Hin6I GCGC 4 cut(s) 156, 196, 686, 1265
HinP1I GCGC 4 cut(s) 156, 196, 686, 1265
HinfI GANTC 5 cut(s) 138, 329, 514, 596, 1406
HpaII CCGG 2 cut(s) 177, 333
HphI GGTGA 4 cut(s) 86, 460, 814, 1214
Hpy166II GTNNAC 3 cut(s) 775, 797, 1279
Hpy188I TCNGA 6 cut(s) 174, 328, 466, 583, 828, 1291
Hpy188III TCNNGA 4 cut(s) 187, 656, 1237, 1403
Hpy8I GTNNAC 3 cut(s) 775, 797, 1279
HpyAV CCTTC 6 cut(s) 265, 488, 738, 934, 964, 1023
HpyCH4III ACNGT 2 cut(s) 769, 791
HpyCH4V TGCA 3 cut(s) 4, 1048, 1193
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 370, 971, 1025
HpyF3I CTNAG 4 cut(s) 19, 171, 714, 1227
Hsp92II CATG 4 cut(s) 538, 704, 1248, 1276
HspAI GCGC 4 cut(s) 156, 196, 686, 1265
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 4 cut(s) 394, 959, 1291, 1318
LguI GCTCTTC 1 cut(s) 1404
Lsp1109I GCAGC 6 cut(s) 140, 348, 546, 922, 1006, 1406
LweI GCATC 2 cut(s) 1057, 1060
MaeI CTAG 2 cut(s) 741, 1415
MaeIII GTNAC 1 cut(s) 344
MalI GATC 4 cut(s) 396, 961, 1293, 1320
MboI GATC 4 cut(s) 394, 959, 1291, 1318
MboII GAAGA 9 cut(s) 67, 202, 315, 913, 1001, 1004, 1146, 1217, 1391
MflI RGATCY 2 cut(s) 959, 1318
MhlI GDGCHC 1 cut(s) 824
MluCI AATT 3 cut(s) 65, 303, 505
MlyI GAGTC 3 cut(s) 132, 523, 605
Mph1103I ATGCAT 1 cut(s) 1050
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 3 cut(s) 696, 864, 1371
MslI CAYNNNNRTG 2 cut(s) 618, 1257
MspA1I CMGCKG 2 cut(s) 361, 377
MspI CCGG 2 cut(s) 177, 333
MspR9I CCNGG 3 cut(s) 334, 564, 834
Mva1269I GAATGC 1 cut(s) 688
MvaI CCWGG 2 cut(s) 564, 834
MwoI GCNNNNNNNGC 4 cut(s) 367, 370, 971, 1025
NciI CCSGG 1 cut(s) 334
NdeI CATATG 1 cut(s) 1044
NdeII GATC 4 cut(s) 394, 959, 1291, 1318
NlaIII CATG 4 cut(s) 538, 704, 1248, 1276
NlaIV GGNNCC 2 cut(s) 181, 1332
NsbI TGCGCA 1 cut(s) 1266
NsiI ATGCAT 1 cut(s) 1050
NspI RCATGY 1 cut(s) 1248
PciI ACATGT 1 cut(s) 1244
PciSI GCTCTTC 1 cut(s) 1404
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PctI GAATGC 1 cut(s) 688
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 2 cut(s) 329, 1406
PleI GAGTC 3 cut(s) 132, 522, 604
PpsI GAGTC 3 cut(s) 132, 522, 604
PpuMI RGGWCCY 1 cut(s) 1330
PscI ACATGT 1 cut(s) 1244
Psp5II RGGWCCY 1 cut(s) 1330
Psp6I CCWGG 2 cut(s) 562, 832
PspEI GGTNACC 1 cut(s) 344
PspFI CCCAGC 4 cut(s) 340, 377, 455, 525
PspGI CCWGG 2 cut(s) 562, 832
PspN4I GGNNCC 2 cut(s) 181, 1332
PspPI GGNCC 4 cut(s) 179, 718, 775, 1330
PspPPI RGGWCCY 1 cut(s) 1330
PstI CTGCAG 1 cut(s) 1195
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 2 cut(s) 959, 1318
RsaI GTAC 2 cut(s) 883, 1056
RsaNI GTAC 2 cut(s) 882, 1055
RseI CAYNNNNRTG 2 cut(s) 618, 1257
SapI GCTCTTC 1 cut(s) 1404
SaqAI TTAA 3 cut(s) 696, 864, 1371
Sau3AI GATC 4 cut(s) 394, 959, 1291, 1318
Sau96I GGNCC 4 cut(s) 179, 718, 775, 1330
SchI GAGTC 3 cut(s) 132, 523, 605
ScrFI CCNGG 3 cut(s) 334, 564, 834
SduI GDGCHC 1 cut(s) 824
SfaNI GCATC 2 cut(s) 1057, 1060
SfcI CTRYAG 1 cut(s) 1191
SinI GGWCC 2 cut(s) 775, 1330
SmiMI CAYNNNNRTG 2 cut(s) 618, 1257
SmlI CTYRAG 2 cut(s) 656, 727
SmoI CTYRAG 2 cut(s) 656, 727
Sse9I AATT 3 cut(s) 65, 303, 505
SspI AATATT 1 cut(s) 544
SspMI CTAG 2 cut(s) 741, 1415
StyD4I CCNGG 3 cut(s) 332, 562, 832
TaaI ACNGT 2 cut(s) 769, 791
TaqI TCGA 2 cut(s) 437, 1236
TaqII GACCGA 1 cut(s) 1379
TasI AATT 3 cut(s) 65, 303, 505
TatI WGTACW 1 cut(s) 1054
TauI GCSGC 6 cut(s) 161, 164, 227, 230, 361, 373
TfiI GAWTC 2 cut(s) 329, 1406
Tru1I TTAA 3 cut(s) 696, 864, 1371
Tru9I TTAA 3 cut(s) 696, 864, 1371
TscAI CASTG 5 cut(s) 622, 785, 981, 991, 1113
TseI GCWGC 6 cut(s) 153, 361, 559, 910, 1019, 1394
TspDTI ATGAA 4 cut(s) 914, 983, 1005, 1398
TspGWI ACGGA 2 cut(s) 255, 497
TspRI CASTG 5 cut(s) 622, 785, 981, 991, 1113
VpaK11BI GGWCC 2 cut(s) 775, 1330
XceI RCATGY 1 cut(s) 1248
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 2 cut(s) 741, 1415
Zsp2I ATGCAT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.