Rh2CG287300

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0005107
Physical Location & Seq
Reverse (-)
32241977 .. 32248284
6308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG287300.1

Sequence Viewer

Length: 906 bp
ATGGCTTTCTCAGTCTCCACAGCCACAGCCAGTTCCAGTCCCCAATTCCATTTCTTCTCTTTTCACTCCAATCATCTATTCCCAGTCTCCCTCTCTCACCCAACGCACACCGCCAGACTCCTCGTCTCCTGCTGCGCCGCCGCAAAGCCTCAGACCGGCCCCGTCAAGAAGAAGCGCTCTCCTCCCTCGCCCACCACCACCACCACCCCCACCCCCGCCAACAACAACAACAACAACAACAGCAAGAAAAAGCGTAAGGCCAAGGATAAGAATACCAGCTTTAGCAAGGTTGAAATTGTGGATGATTTCGGGTCTTCAGATTCCGGGTCGCTGGGTTACCATCCTACGCCGCTGCCGAAGCCGCCCGCTGGGTTTGTAGTGGATGATCACGGCAGGGTGCTTATGGCTTCGTCCAAGCGAATTGCCTCGATTGTTGATCCTACCAATAATTTCCCATTAGAGTGCGTTATAAGGAGAGTGTTCAGAAGCTCACGAGGGGATGAATGTATGCTGCTTTGCCCTGTTGACACGCCTGTTCAGATATTAAAGAGCACCAATATAGATGGTTGGTCAGCGGTCAGTGATGAAGAAGTTGAAGCTATTCTTCCTTCTGCTGCTTATGCCCTTGCCAAGATACATATGCATCTTGTACATAGCGGATTTTGCTATACAGCAAGGGGAGGATTTTGTTACTCAGAAGATGACATATTTGATTTTCGCACAGATGATGGTGAAGATGTAGATGGCTTGCCAACCGAGGGTGTCGAGATTACATGTTTCCATTTGGATGGTGCGCATTACATGGTTTACACACCCTCTGATCCACTTCTCTTTGTTGCTGTAAAGGATAATGACGGTCTATTGCAAATTGCTGATGATGTAAGTAATGCACTCATTTGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

301

Amino Acids

32.64

Weight (kDa)

5.92

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 213 - 293 1.4e-18 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 470
Acc16I TGCGCA 1 cut(s) 795
AciI CCGC 9 cut(s) 111, 138, 141, 216, 350, 362, 366, 575, 657
AclWI GGATC 2 cut(s) 431, 815
AcuI CTGAAG 1 cut(s) 300
AfaI GTAC 1 cut(s) 651
AfeI AGCGCT 1 cut(s) 176
AfiI CCNNNNNNNGG 3 cut(s) 155, 368, 758
AflIII ACRYGT 1 cut(s) 773
AgsI TTSAA 2 cut(s) 293, 596
AhdI GACNNNNNGTC 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 3 cut(s) 279, 489, 599
AluI AGCT 3 cut(s) 279, 489, 599
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 3 cut(s) 19, 91, 130
AlwI GGATC 2 cut(s) 431, 815
Aor51HI AGCGCT 1 cut(s) 176
AoxI GGCC 2 cut(s) 157, 258
ApeKI GCWGC 4 cut(s) 132, 352, 511, 614
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 3 cut(s) 137, 177, 796
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 89, 743
BauI CACGAG 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 119, 339, 498, 601
BccI CCATC 5 cut(s) 348, 557, 722, 737, 782
BceAI ACGGC 1 cut(s) 406
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 3 cut(s) 19, 91, 130
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 8 cut(s) 133, 138, 141, 350, 353, 362, 512, 615
BlsI GCNGC 8 cut(s) 134, 139, 142, 351, 354, 363, 513, 616
Bme1390I CCNGG 1 cut(s) 325
BmeRI GACNNNNNGTC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 325
BmrI ACTGGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 652
BmuI ACTGGG 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 325
BsaJI CCNNGG 2 cut(s) 261, 756
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 368, 758
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 3 cut(s) 30, 36, 83
BseDI CCNNGG 2 cut(s) 261, 756
BseGI GGATG 5 cut(s) 307, 340, 388, 505, 793
BseLI CCNNNNNNNGG 3 cut(s) 155, 368, 758
BseMII CTCAG 3 cut(s) 24, 164, 708
BseNI ACTGG 3 cut(s) 30, 36, 83
BseRI GAGGAG 2 cut(s) 110, 171
BseXI GCAGC 4 cut(s) 119, 339, 498, 601
BseYI CCCAGC 2 cut(s) 331, 368
BshFI GGCC 2 cut(s) 159, 260
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 2 cut(s) 156, 324
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 3 cut(s) 155, 368, 758
BsmAI GTCTC 3 cut(s) 19, 91, 130
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 2 cut(s) 159, 260
Bsp1286I GDGCHC 1 cut(s) 554
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 3 cut(s) 385, 436, 820
BspACI CCGC 9 cut(s) 111, 138, 141, 216, 350, 362, 366, 575, 657
BspANI GGCC 2 cut(s) 159, 260
BspCNI CTCAG 3 cut(s) 23, 163, 707
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 2 cut(s) 431, 815
BsrFI RCCGGY 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 3 cut(s) 30, 36, 83
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 261, 756
BssMI GATC 3 cut(s) 385, 436, 820
BssSI CACGAG 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 492
Bst4CI ACNGT 1 cut(s) 857
BstAUI TGTACA 1 cut(s) 649
BstC8I GCNNGC 2 cut(s) 366, 749
BstDEI CTNAG 3 cut(s) 10, 150, 694
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 5 cut(s) 307, 340, 388, 505, 793
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 3 cut(s) 137, 177, 796
BstKTI GATC 3 cut(s) 388, 439, 823
BstMAI GTCTC 3 cut(s) 19, 91, 130
BstMBI GATC 3 cut(s) 385, 436, 820
BstMWI GCNNNNNNNGC 4 cut(s) 358, 361, 620, 663
BstNSI RCATGY 1 cut(s) 777
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 323
BstV1I GCAGC 4 cut(s) 119, 339, 498, 601
BstV2I GAAGAC 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 788
BsuRI GGCC 2 cut(s) 159, 260
BtsCI GGATG 5 cut(s) 307, 340, 388, 505, 793
BtsIMutI CAGTG 1 cut(s) 586
Cac8I GCNNGC 2 cut(s) 366, 749
CfoI GCGC 3 cut(s) 137, 177, 796
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 650
CviAII CATG 2 cut(s) 774, 802
CviQI GTAC 1 cut(s) 650
DdeI CTNAG 3 cut(s) 10, 150, 694
DpnI GATC 3 cut(s) 387, 438, 822
DpnII GATC 3 cut(s) 385, 436, 820
DriI GACNNNNNGTC 1 cut(s) 122
Eam1105I GACNNNNNGTC 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 261
Eco47III AGCGCT 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 300
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 261
EcoT22I ATGCAT 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 2 cut(s) 777, 805
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 773, 801
FauI CCCGC 2 cut(s) 223, 373
FauNDI CATATG 1 cut(s) 639
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 8 cut(s) 133, 138, 141, 350, 353, 362, 512, 615
FokI GGATG 5 cut(s) 314, 327, 395, 512, 800
Fsp4HI GCNGC 8 cut(s) 133, 138, 141, 350, 353, 362, 512, 615
FspAI RTGCGCAY 1 cut(s) 795
FspI TGCGCA 1 cut(s) 795
GlaI GCGC 3 cut(s) 136, 176, 795
GluI GCNGC 8 cut(s) 133, 138, 141, 350, 353, 362, 512, 615
GsaI CCCAGC 2 cut(s) 335, 372
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 2 cut(s) 159, 260
HapII CCGG 2 cut(s) 156, 324
HhaI GCGC 3 cut(s) 137, 177, 796
Hin1II CATG 2 cut(s) 777, 805
Hin6I GCGC 3 cut(s) 135, 175, 794
HinP1I GCGC 3 cut(s) 135, 175, 794
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 2 cut(s) 117, 320
HpaII CCGG 2 cut(s) 156, 324
HphI GGTGA 2 cut(s) 89, 743
Hpy166II GTNNAC 2 cut(s) 526, 808
Hpy188I TCNGA 6 cut(s) 153, 319, 485, 540, 697, 820
Hpy188III TCNNGA 3 cut(s) 166, 492, 766
Hpy8I GTNNAC 2 cut(s) 526, 808
HpyAV CCTTC 1 cut(s) 618
HpyCH4III ACNGT 1 cut(s) 857
HpyCH4V TGCA 3 cut(s) 643, 865, 890
HpyF10VI GCNNNNNNNGC 4 cut(s) 358, 361, 620, 663
HpyF3I CTNAG 3 cut(s) 10, 150, 694
Hsp92II CATG 2 cut(s) 777, 805
HspAI GCGC 3 cut(s) 135, 175, 794
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 3 cut(s) 385, 436, 820
Lsp1109I GCAGC 4 cut(s) 119, 339, 498, 601
LweI GCATC 1 cut(s) 652
MaeIII GTNAC 2 cut(s) 335, 689
MalI GATC 3 cut(s) 387, 438, 822
MboI GATC 3 cut(s) 385, 436, 820
MboII GAAGA 7 cut(s) 46, 181, 306, 596, 599, 710, 746
MhlI GDGCHC 1 cut(s) 554
MluCI AATT 5 cut(s) 44, 294, 420, 448, 867
MlyI GAGTC 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 1 cut(s) 545
MslI CAYNNNNRTG 2 cut(s) 460, 786
MspA1I CMGCKG 3 cut(s) 352, 368, 575
MspI CCGG 2 cut(s) 156, 324
MspR9I CCNGG 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 358, 361, 620, 663
NciI CCSGG 1 cut(s) 325
NdeI CATATG 1 cut(s) 639
NdeII GATC 3 cut(s) 385, 436, 820
NlaIII CATG 2 cut(s) 777, 805
NlaIV GGNNCC 1 cut(s) 160
NsbI TGCGCA 1 cut(s) 795
NsiI ATGCAT 1 cut(s) 645
NspI RCATGY 1 cut(s) 777
PciI ACATGT 1 cut(s) 773
PcsI WCGNNNNNNNCGW 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 1 cut(s) 320
PkrI GCNGC 8 cut(s) 134, 139, 142, 351, 354, 363, 513, 616
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PscI ACATGT 1 cut(s) 773
PsiI TTATAA 1 cut(s) 470
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 2 cut(s) 331, 368
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 158
RsaI GTAC 1 cut(s) 651
RsaNI GTAC 1 cut(s) 650
RseI CAYNNNNRTG 2 cut(s) 460, 786
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 8 cut(s) 133, 138, 141, 350, 353, 362, 512, 615
Sau3AI GATC 3 cut(s) 385, 436, 820
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 554
SetI ASST 4 cut(s) 281, 291, 491, 601
SfaNI GCATC 1 cut(s) 652
SmiMI CAYNNNNRTG 2 cut(s) 460, 786
Sse9I AATT 5 cut(s) 44, 294, 420, 448, 867
SsiI CCGC 9 cut(s) 111, 138, 141, 216, 350, 362, 366, 575, 657
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 857
TaqI TCGA 2 cut(s) 428, 765
TasI AATT 5 cut(s) 44, 294, 420, 448, 867
TatI WGTACW 1 cut(s) 649
TauI GCSGC 4 cut(s) 140, 143, 352, 364
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 1 cut(s) 586
TseI GCWGC 4 cut(s) 132, 352, 511, 614
TspDTI ATGAA 2 cut(s) 516, 600
TspRI CASTG 1 cut(s) 586
XceI RCATGY 1 cut(s) 777
XmnI GAANNNNTTC 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.