RLG00000018711

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005107
Physical Location & Seq
Reverse (-)
34275256 .. 34280925
5670 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018711

Sequence Viewer

Length: 981 bp
ATGGCTTTCTCAGTCTCCACAGCCACAGCCAGAGCCAGAGCCACAGCCAGTCCCCAATTCCATTTCTTCTCTTTTCACTTCAATCACCTATTCCCAGTCTCCCTCTCTCTCCCAACCCACACCGCCAGACTCCTCATCTCCTGCTGCGCCGCCGCAAAGCCTCAGACCGGCCCCGTCAAGAAGAAACGCTCTCCTCCCTCGCCCACCACCCCCGCCAACAACAACAACAACAGCAAGAAAAAGCGTAAGGCCAAGGATAAGAATACCAGCTTTAGCAAGGTTGAAATTGTGGATGATTTCGGGTCTTCAGATTCCGGGTCGCTGGGTTACCATCCTACGCCGCTGCCGAAGCCGCCCGCTGGGTTTGTAGTGGATGATCACGGCAGGGTGCTTATGGCTTCGTCCAAGCGAATTGCCTCGATTGTTGATCCTACCAATAATTTCCCACTAGAGTGTGTTATAAGGAGAGTGTTCAGAAGCTCACGAGGGGATGAATGTATGCTGCTTTGCCCTGTTGACACGCCTGTTCAGATATTAAAGAGCACCAATATAGATGGTTGGTCAGCGGTCAGTGATGAAGAAGTTGAGGCTATTCTTCCCTCTGCTGCTTATGCCCTTGCCAAGATACATATGCATCTTGTACATAGCGGCTTTTGTTATACAGCAAGGGGAGGGTTTTGTTACTCAGAAGATGACATATTTGATTTTCGCACAGATGATGGTGAAGATGTAGATGGCTTGCCAACCGAGGGTGTTGAGATTACATGTTTCCATTTGGATGGTGCGCATTACATGGTTTATACACCCTCTGACCCACTTCTCTTTGTTGCTGTAAAGGATAATGATGGTCTATTGCAAATTGCTGATGATGATCTGCTGGAGGATCCTGCTATCATTAGTGCCATAGATGAGGAGACCGAGTTTAACGCATTGGTGGAGGAAGAAGCAGCTCTTCTTGATTCATTGCTAGGCAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

327

Amino Acids

35.52

Weight (kDa)

5.07

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 210 - 304 1.2e-20 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 461
Acc16I TGCGCA 1 cut(s) 786
AciI CCGC 9 cut(s) 123, 150, 153, 213, 341, 353, 357, 566, 648
AclWI GGATC 3 cut(s) 422, 878, 891
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 3 cut(s) 167, 359, 749
AflIII ACRYGT 1 cut(s) 764
AgsI TTSAA 2 cut(s) 82, 284
AleI CACNNNNGTG 1 cut(s) 451
AluBI AGCT 3 cut(s) 270, 480, 950
AluI AGCT 3 cut(s) 270, 480, 950
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 3 cut(s) 19, 103, 908
AlwI GGATC 3 cut(s) 422, 878, 891
AoxI GGCC 2 cut(s) 169, 249
ApeKI GCWGC 5 cut(s) 144, 343, 502, 605, 947
AspLEI GCGC 2 cut(s) 149, 787
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 2 cut(s) 77, 734
BamHI GGATCC 1 cut(s) 883
BauI CACGAG 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 5 cut(s) 131, 330, 489, 592, 959
BccI CCATC 6 cut(s) 339, 548, 713, 728, 773, 839
BceAI ACGGC 1 cut(s) 397
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 1 cut(s) 316
BcoDI GTCTC 3 cut(s) 19, 103, 908
BfaI CTAG 2 cut(s) 449, 968
Bme1390I CCNGG 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 172, 885
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 643
BmuI ACTGGG 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 316
BsaBI GATNNNNATC 1 cut(s) 870
BsaI GGTCTC 1 cut(s) 908
BsaJI CCNNGG 2 cut(s) 252, 747
BsaXI ACNNNNNCTCC 2 cut(s) 905, 935
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 359, 749
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 48, 95
Bse3DI GCAATG 1 cut(s) 962
Bse8I GATNNNNATC 1 cut(s) 870
BseDI CCNNGG 2 cut(s) 252, 747
BseGI GGATG 5 cut(s) 298, 331, 379, 496, 784
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 3 cut(s) 167, 359, 749
BseMI GCAATG 1 cut(s) 962
BseMII CTCAG 3 cut(s) 24, 176, 699
BseNI ACTGG 2 cut(s) 48, 95
BseRI GAGGAG 3 cut(s) 122, 183, 926
BseXI GCAGC 5 cut(s) 131, 330, 489, 592, 959
BseYI CCCAGC 2 cut(s) 322, 359
BshFI GGCC 2 cut(s) 171, 251
BsiHKAI GWGCWC 1 cut(s) 545
BsiSI CCGG 2 cut(s) 168, 315
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 3 cut(s) 167, 359, 749
BsmAI GTCTC 3 cut(s) 19, 103, 908
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 2 cut(s) 171, 251
Bso31I GGTCTC 1 cut(s) 908
Bsp1286I GDGCHC 1 cut(s) 545
Bsp1407I TGTACA 1 cut(s) 640
Bsp143I GATC 4 cut(s) 376, 427, 871, 883
BspACI CCGC 9 cut(s) 123, 150, 153, 213, 341, 353, 357, 566, 648
BspANI GGCC 2 cut(s) 171, 251
BspCNI CTCAG 3 cut(s) 23, 175, 698
BspLI GGNNCC 2 cut(s) 172, 885
BspPI GGATC 3 cut(s) 422, 878, 891
BspQI GCTCTTC 1 cut(s) 957
BspTNI GGTCTC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 962
BsrFI RCCGGY 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 640
BsrI ACTGG 2 cut(s) 48, 95
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 252, 747
BssMI GATC 4 cut(s) 376, 427, 871, 883
BssSI CACGAG 1 cut(s) 483
BssT1I CCWWGG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 483
Bst6I CTCTTC 1 cut(s) 957
BstAUI TGTACA 1 cut(s) 640
BstC8I GCNNGC 2 cut(s) 357, 740
BstDEI CTNAG 3 cut(s) 10, 162, 685
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 5 cut(s) 298, 331, 379, 496, 784
BstHHI GCGC 2 cut(s) 149, 787
BstKTI GATC 4 cut(s) 379, 430, 874, 886
BstMAI GTCTC 3 cut(s) 19, 103, 908
BstMBI GATC 4 cut(s) 376, 427, 871, 883
BstMWI GCNNNNNNNGC 3 cut(s) 349, 352, 611
BstNSI RCATGY 1 cut(s) 768
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 314
BstV1I GCAGC 5 cut(s) 131, 330, 489, 592, 959
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 883
BstXI CCANNNNNNTGG 1 cut(s) 779
BstYI RGATCY 1 cut(s) 883
BsuRI GGCC 2 cut(s) 171, 251
BtsCI GGATG 5 cut(s) 298, 331, 379, 496, 784
BtsIMutI CAGTG 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 357, 740
CfoI GCGC 2 cut(s) 149, 787
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 641
CviAII CATG 2 cut(s) 765, 793
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 3 cut(s) 10, 162, 685
DpnI GATC 4 cut(s) 378, 429, 873, 885
DpnII GATC 4 cut(s) 376, 427, 871, 883
Eam1104I CTCTTC 1 cut(s) 957
EarI CTCTTC 1 cut(s) 957
Eco130I CCWWGG 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 291
Eco91I GGTNACC 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 252
EcoT22I ATGCAT 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 768, 796
FalI AAGNNNNNCTT 2 cut(s) 936, 968
FaqI GGGAC 1 cut(s) 36
FatI CATG 2 cut(s) 764, 792
FauI CCCGC 2 cut(s) 220, 364
FauNDI CATATG 1 cut(s) 630
FbaI TGATCA 1 cut(s) 376
FokI GGATG 5 cut(s) 305, 318, 386, 503, 791
FspAI RTGCGCAY 1 cut(s) 786
FspBI CTAG 2 cut(s) 449, 968
FspI TGCGCA 1 cut(s) 786
GlaI GCGC 2 cut(s) 148, 786
GsaI CCCAGC 2 cut(s) 326, 363
GsuI CTGGAG 1 cut(s) 899
HaeIII GGCC 2 cut(s) 171, 251
HapII CCGG 2 cut(s) 168, 315
HhaI GCGC 2 cut(s) 149, 787
Hin1II CATG 2 cut(s) 768, 796
Hin6I GCGC 2 cut(s) 147, 785
HinP1I GCGC 2 cut(s) 147, 785
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HinfI GANTC 3 cut(s) 129, 311, 959
HpaII CCGG 2 cut(s) 168, 315
HphI GGTGA 2 cut(s) 77, 734
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 6 cut(s) 165, 310, 476, 531, 688, 811
Hpy188III TCNNGA 3 cut(s) 178, 483, 956
Hpy8I GTNNAC 1 cut(s) 517
HpyCH4V TGCA 2 cut(s) 634, 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 349, 352, 611
HpyF3I CTNAG 3 cut(s) 10, 162, 685
Hsp92II CATG 2 cut(s) 768, 796
HspAI GCGC 2 cut(s) 147, 785
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 376, 427, 871, 883
LguI GCTCTTC 1 cut(s) 957
Lsp1109I GCAGC 5 cut(s) 131, 330, 489, 592, 959
LweI GCATC 1 cut(s) 643
MaeI CTAG 2 cut(s) 449, 968
MaeIII GTNAC 2 cut(s) 326, 680
MalI GATC 4 cut(s) 378, 429, 873, 885
MboI GATC 4 cut(s) 376, 427, 871, 883
MboII GAAGA 9 cut(s) 58, 193, 297, 587, 590, 701, 737, 944, 953
MflI RGATCY 1 cut(s) 883
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 5 cut(s) 56, 285, 411, 439, 858
MlyI GAGTC 1 cut(s) 123
Mph1103I ATGCAT 1 cut(s) 636
MseI TTAA 2 cut(s) 536, 924
MslI CAYNNNNRTG 2 cut(s) 451, 777
MspA1I CMGCKG 3 cut(s) 343, 359, 566
MspI CCGG 2 cut(s) 168, 315
MspR9I CCNGG 1 cut(s) 316
MwoI GCNNNNNNNGC 3 cut(s) 349, 352, 611
NciI CCSGG 1 cut(s) 316
NdeI CATATG 1 cut(s) 630
NdeII GATC 4 cut(s) 376, 427, 871, 883
NlaIII CATG 2 cut(s) 768, 796
NlaIV GGNNCC 2 cut(s) 172, 885
NsbI TGCGCA 1 cut(s) 786
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 768
OliI CACNNNNGTG 1 cut(s) 451
PciI ACATGT 1 cut(s) 764
PciSI GCTCTTC 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PfeI GAWTC 2 cut(s) 311, 959
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PscI ACATGT 1 cut(s) 764
PsiI TTATAA 1 cut(s) 461
PspEI GGTNACC 1 cut(s) 326
PspFI CCCAGC 2 cut(s) 322, 359
PspN4I GGNNCC 2 cut(s) 172, 885
PspPI GGNCC 1 cut(s) 170
PsuI RGATCY 1 cut(s) 883
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
RseI CAYNNNNRTG 2 cut(s) 451, 777
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 2 cut(s) 536, 924
Sau3AI GATC 4 cut(s) 376, 427, 871, 883
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 1 cut(s) 545
SetI ASST 5 cut(s) 90, 272, 282, 482, 952
SfaNI GCATC 1 cut(s) 643
SmiMI CAYNNNNRTG 2 cut(s) 451, 777
Sse9I AATT 5 cut(s) 56, 285, 411, 439, 858
SsiI CCGC 9 cut(s) 123, 150, 153, 213, 341, 353, 357, 566, 648
SspMI CTAG 2 cut(s) 449, 968
StyD4I CCNGG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 252
TaqI TCGA 1 cut(s) 419
TaqII GACCGA 1 cut(s) 932
TasI AATT 5 cut(s) 56, 285, 411, 439, 858
TatI WGTACW 1 cut(s) 640
TauI GCSGC 5 cut(s) 152, 155, 343, 355, 651
TfiI GAWTC 2 cut(s) 311, 959
Tru1I TTAA 2 cut(s) 536, 924
Tru9I TTAA 2 cut(s) 536, 924
TscAI CASTG 1 cut(s) 577
TseI GCWGC 5 cut(s) 144, 343, 502, 605, 947
TspDTI ATGAA 3 cut(s) 507, 591, 951
TspRI CASTG 1 cut(s) 577
XceI RCATGY 1 cut(s) 768
XspI CTAG 2 cut(s) 449, 968
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.