Rw2G024120

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005107
Physical Location & Seq
Reverse (-)
35525368 .. 35531177
5810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024120.1

Sequence Viewer

Length: 978 bp
ATGGCTTTCTCAGTCTCCACAGCCACAGCCACAGCCAGTTCCAGTCCCCAATTCCATTTCTTCTCTGTTCACTCCAATCACCAATTCCCAGTCTCCCTCTCTCTCCCAACCCACACCGCCAGACTCCTCGTCTCCTGCTGCGCCGCCGCAAAGCCTCAGACCGGCCCCGTCAAGAAGAAGCGCTCTCCTCCCTCACCCACCACCACCACCGCCGCCAACAACAACAGCAACAACAACAGAAAAAAGCGTAAGACAACAACAACAGCAAGAAAAAGCGTTGAAATTGTGGATGATTTCGGGTCTTCAGATTCCGGGTCGCTGGGTTACCATCCTACGCCGCTGCCGAAGCCGCCCGCTGGGTTTGTAGTGGATGATCACGGCAGGGTGCTTATGGCTTCGTCCAAGCGAATTGCCTCGATTGTTGATCCTACCAATAATTTCCCATTAGAGTGCGTTATAAGGAGAGTGTTCAGAAGCTCACGAGGGGATGAATGTATGCTGCTTTGCCCTGTTGACACGCCTGTTCAGATATTAAAGAGCACCAATATAGATGGTTGGTCAGCGGTCAGTGATGAAGAAGTTGAAGCTATTCTTCCTTCTGCTGCTTATGCCCTTGCCAAGATACATATGCATCTTGTACATAGCGGATTTTGCTATACAGCAAGGGGAGGATTTTGTTACTCAGAAGATGACATATTTGATTTTCGCACAGATGATGGTGAAGATGTAGATGGCTTGCCAACCGAGGGTGTTGAGATTACATGTTTCCATTTGGATGGTGCGCATTATATGGTTTACACACCCTCTGATCCACTTCTCTTTGTTGCTGTAAAGGATGATGACGGTCTATTGCAAATTGCTGATGATGATCTGCTGGAGGACCCTGCCATCATTAGCGCCATAGATGAGGAGACCGAGTTTAACGCATTGGTGGAGGAAGAAGCAGCTCTTCTTGATTCATTGCTAGGAAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

325

Amino Acids

35.19

Weight (kDa)

4.89

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 209 - 303 1.4e-20 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 458
Acc16I TGCGCA 1 cut(s) 783
AclWI GGATC 2 cut(s) 419, 803
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 1 cut(s) 639
AfeI AGCGCT 1 cut(s) 182
AfiI CCNNNNNNNGG 3 cut(s) 161, 356, 746
AflIII ACRYGT 1 cut(s) 761
AgsI TTSAA 2 cut(s) 281, 584
AhdI GACNNNNNGTC 1 cut(s) 128
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AluBI AGCT 3 cut(s) 477, 587, 947
AluI AGCT 3 cut(s) 477, 587, 947
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 4 cut(s) 19, 97, 136, 905
AlwI GGATC 2 cut(s) 419, 803
Aor51HI AGCGCT 1 cut(s) 182
AoxI GGCC 1 cut(s) 163
ApeKI GCWGC 5 cut(s) 138, 340, 499, 602, 944
Asp700I GAANNNNTTC 1 cut(s) 588
AspLEI GCGC 4 cut(s) 143, 183, 784, 899
AspS9I GGNCC 2 cut(s) 164, 880
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 3 cut(s) 71, 186, 731
AvaII GGWCC 1 cut(s) 880
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 542
BbvI GCAGC 5 cut(s) 125, 327, 486, 589, 956
BccI CCATC 6 cut(s) 336, 545, 710, 725, 770, 896
BceAI ACGGC 1 cut(s) 394
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 4 cut(s) 19, 97, 136, 905
BfaI CTAG 1 cut(s) 965
BfoI RGCGCY 2 cut(s) 184, 900
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 880
BmeRI GACNNNNNGTC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 164, 880
BmiI GGNNCC 2 cut(s) 166, 882
BmrFI CCNGG 1 cut(s) 313
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 896
BpuMI CCSGG 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 1 cut(s) 744
BsaXI ACNNNNNCTCC 2 cut(s) 902, 932
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 356, 746
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 3 cut(s) 36, 42, 89
Bse3DI GCAATG 1 cut(s) 959
Bse8I GATNNNNATC 1 cut(s) 867
BseDI CCNNGG 1 cut(s) 744
BseGI GGATG 6 cut(s) 295, 328, 376, 493, 781, 841
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 3 cut(s) 161, 356, 746
BseMI GCAATG 1 cut(s) 959
BseMII CTCAG 3 cut(s) 24, 170, 696
BseNI ACTGG 3 cut(s) 36, 42, 89
BseRI GAGGAG 3 cut(s) 116, 177, 923
BseXI GCAGC 5 cut(s) 125, 327, 486, 589, 956
BseYI CCCAGC 2 cut(s) 319, 356
BshFI GGCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 542
BsiSI CCGG 2 cut(s) 162, 312
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 3 cut(s) 161, 356, 746
BsmAI GTCTC 4 cut(s) 19, 97, 136, 905
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 1 cut(s) 165
Bso31I GGTCTC 1 cut(s) 905
Bsp1286I GDGCHC 1 cut(s) 542
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 4 cut(s) 373, 424, 808, 868
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 3 cut(s) 23, 169, 695
BspLI GGNNCC 2 cut(s) 166, 882
BspPI GGATC 2 cut(s) 419, 803
BspQI GCTCTTC 1 cut(s) 954
BspTNI GGTCTC 1 cut(s) 905
BsrDI GCAATG 1 cut(s) 959
BsrFI RCCGGY 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 3 cut(s) 36, 42, 89
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 744
BssMI GATC 4 cut(s) 373, 424, 808, 868
BssSI CACGAG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 845
Bst6I CTCTTC 1 cut(s) 954
BstAUI TGTACA 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 354, 737
BstDEI CTNAG 3 cut(s) 10, 156, 682
BstEII GGTNACC 1 cut(s) 323
BstF5I GGATG 6 cut(s) 295, 328, 376, 493, 781, 841
BstH2I RGCGCY 2 cut(s) 184, 900
BstHHI GCGC 4 cut(s) 143, 183, 784, 899
BstKTI GATC 4 cut(s) 376, 427, 811, 871
BstMAI GTCTC 4 cut(s) 19, 97, 136, 905
BstMBI GATC 4 cut(s) 373, 424, 808, 868
BstMWI GCNNNNNNNGC 4 cut(s) 346, 349, 608, 651
BstNSI RCATGY 1 cut(s) 765
BstPI GGTNACC 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 311
BstV1I GCAGC 5 cut(s) 125, 327, 486, 589, 956
BstV2I GAAGAC 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 776
BsuRI GGCC 1 cut(s) 165
BtsCI GGATG 6 cut(s) 295, 328, 376, 493, 781, 841
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 2 cut(s) 354, 737
CfoI GCGC 4 cut(s) 143, 183, 784, 899
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 164, 880
Csp6I GTAC 1 cut(s) 638
CviAII CATG 1 cut(s) 762
CviQI GTAC 1 cut(s) 638
DdeI CTNAG 3 cut(s) 10, 156, 682
DpnI GATC 4 cut(s) 375, 426, 810, 870
DpnII GATC 4 cut(s) 373, 424, 808, 868
DriI GACNNNNNGTC 1 cut(s) 128
Eam1104I CTCTTC 1 cut(s) 954
Eam1105I GACNNNNNGTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 954
Eco31I GGTCTC 1 cut(s) 905
Eco47I GGWCC 1 cut(s) 880
Eco47III AGCGCT 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 288
Eco91I GGTNACC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 880
EcoO65I GGTNACC 1 cut(s) 323
EcoT22I ATGCAT 1 cut(s) 633
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 1 cut(s) 765
FalI AAGNNNNNCTT 4 cut(s) 576, 608, 933, 965
FaqI GGGAC 1 cut(s) 30
FatI CATG 1 cut(s) 761
FauI CCCGC 1 cut(s) 361
FauNDI CATATG 1 cut(s) 627
FbaI TGATCA 1 cut(s) 373
FokI GGATG 6 cut(s) 302, 315, 383, 500, 788, 848
FspAI RTGCGCAY 1 cut(s) 783
FspBI CTAG 1 cut(s) 965
FspI TGCGCA 1 cut(s) 783
GlaI GCGC 4 cut(s) 142, 182, 783, 898
GsaI CCCAGC 2 cut(s) 323, 360
GsuI CTGGAG 1 cut(s) 896
HaeII RGCGCY 2 cut(s) 184, 900
HaeIII GGCC 1 cut(s) 165
HapII CCGG 2 cut(s) 162, 312
HhaI GCGC 4 cut(s) 143, 183, 784, 899
Hin1II CATG 1 cut(s) 765
Hin6I GCGC 4 cut(s) 141, 181, 782, 897
HinP1I GCGC 4 cut(s) 141, 181, 782, 897
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HinfI GANTC 3 cut(s) 123, 308, 956
HpaII CCGG 2 cut(s) 162, 312
HphI GGTGA 3 cut(s) 71, 186, 731
Hpy166II GTNNAC 3 cut(s) 70, 514, 796
Hpy188I TCNGA 6 cut(s) 159, 307, 473, 528, 685, 808
Hpy188III TCNNGA 3 cut(s) 172, 480, 953
Hpy8I GTNNAC 3 cut(s) 70, 514, 796
HpyAV CCTTC 1 cut(s) 606
HpyCH4III ACNGT 1 cut(s) 845
HpyCH4V TGCA 2 cut(s) 631, 853
HpyF10VI GCNNNNNNNGC 4 cut(s) 346, 349, 608, 651
HpyF3I CTNAG 3 cut(s) 10, 156, 682
Hsp92II CATG 1 cut(s) 765
HspAI GCGC 4 cut(s) 141, 181, 782, 897
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 373, 424, 808, 868
LguI GCTCTTC 1 cut(s) 954
Lsp1109I GCAGC 5 cut(s) 125, 327, 486, 589, 956
LweI GCATC 1 cut(s) 640
MaeI CTAG 1 cut(s) 965
MaeIII GTNAC 2 cut(s) 323, 677
MalI GATC 4 cut(s) 375, 426, 810, 870
MboI GATC 4 cut(s) 373, 424, 808, 868
MboII GAAGA 9 cut(s) 52, 187, 294, 584, 587, 698, 734, 941, 950
MhlI GDGCHC 1 cut(s) 542
MluCI AATT 6 cut(s) 50, 83, 282, 408, 436, 855
MlyI GAGTC 1 cut(s) 117
Mph1103I ATGCAT 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 588
MseI TTAA 2 cut(s) 533, 921
MslI CAYNNNNRTG 2 cut(s) 448, 774
MspA1I CMGCKG 3 cut(s) 340, 356, 563
MspI CCGG 2 cut(s) 162, 312
MspR9I CCNGG 1 cut(s) 313
MwoI GCNNNNNNNGC 4 cut(s) 346, 349, 608, 651
NciI CCSGG 1 cut(s) 313
NdeI CATATG 1 cut(s) 627
NdeII GATC 4 cut(s) 373, 424, 808, 868
NlaIII CATG 1 cut(s) 765
NlaIV GGNNCC 2 cut(s) 166, 882
NsbI TGCGCA 1 cut(s) 783
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 765
PciI ACATGT 1 cut(s) 761
PciSI GCTCTTC 1 cut(s) 954
PcsI WCGNNNNNNNCGW 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 308, 956
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
PpuMI RGGWCCY 1 cut(s) 880
PscI ACATGT 1 cut(s) 761
PsiI TTATAA 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 880
PspEI GGTNACC 1 cut(s) 323
PspFI CCCAGC 2 cut(s) 319, 356
PspN4I GGNNCC 2 cut(s) 166, 882
PspPI GGNCC 2 cut(s) 164, 880
PspPPI RGGWCCY 1 cut(s) 880
RsaI GTAC 1 cut(s) 639
RsaNI GTAC 1 cut(s) 638
RseI CAYNNNNRTG 2 cut(s) 448, 774
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 2 cut(s) 533, 921
Sau3AI GATC 4 cut(s) 373, 424, 808, 868
Sau96I GGNCC 2 cut(s) 164, 880
SchI GAGTC 1 cut(s) 117
ScrFI CCNGG 1 cut(s) 313
SduI GDGCHC 1 cut(s) 542
SetI ASST 3 cut(s) 479, 589, 949
SfaNI GCATC 1 cut(s) 640
SinI GGWCC 1 cut(s) 880
SmiMI CAYNNNNRTG 2 cut(s) 448, 774
Sse9I AATT 6 cut(s) 50, 83, 282, 408, 436, 855
SspMI CTAG 1 cut(s) 965
StyD4I CCNGG 1 cut(s) 311
TaaI ACNGT 1 cut(s) 845
TaqI TCGA 1 cut(s) 416
TaqII GACCGA 1 cut(s) 929
TasI AATT 6 cut(s) 50, 83, 282, 408, 436, 855
TatI WGTACW 1 cut(s) 637
TauI GCSGC 5 cut(s) 146, 149, 215, 340, 352
TfiI GAWTC 2 cut(s) 308, 956
Tru1I TTAA 2 cut(s) 533, 921
Tru9I TTAA 2 cut(s) 533, 921
TscAI CASTG 1 cut(s) 574
TseI GCWGC 5 cut(s) 138, 340, 499, 602, 944
TspDTI ATGAA 3 cut(s) 504, 588, 948
TspRI CASTG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 880
XceI RCATGY 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 1 cut(s) 965
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.