AT4G35380

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002933
Physical Location & Seq
Forward (+)
16819792 .. 16826147
6356 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35380.1

Sequence Viewer

Length: 5121 bp
ATGTCAACGTCACAAACCCTCGGCGGCGCCACACGTTGTGGCCGGATCATAGGTCCATCATTAGACAAGATCATAAAAAACGCTGCGTGGCGTAAACACACGTACCTTGTCTCTTCATGTAAATCCGTTCTCGACAAGCTCGAATCTCTTCCCGACGACTTCCACGATCCTTCCTCCGTCGTCTCTGGCCTCGCCGCATCAGATGCAGATTCCGTTCTCCAACCTTTTCTCTTATCTCTCGAAACAGCTTATTCAAAAGTCGTCGAGCCTTCTCTAGATTGCGCTTTTAAACTCTTCTCTCTCTCAATCCTCCGCGGTGAGATCCAATCATCAAAACAAGATTCAATCCTCTTCAAACTCGTCAACGCCGTCTCCAAAGTCGGTGCTATAGCCGAAGAACCGATTCAACTCGCTGTTCTTAGAGTTCTTCTCGCTGCCGTTAGATCTCCATGTATCTTGATCCGTGGTGATTGCTTGTTACACGTTGTCAAGACTTGTTATAATATTTACCTTGGTGGCTTGAGCGGCACGACACAGATCTGTGCTAAATCTGTCTTAGCTCAGATGATGCTTGTGATCTTCACAAGATCTGAAGAAGATTCGCTTGATGTTTCTGTCAAAACGATTTACGTCAACGAGTTATTAACTTTCACGGACAAGAGTGTTAATGAAGGAAGCTCTGTTTATTTCTGTCAAGGGTTTGTGAATGAAGTCATGGCTGCAGGACAAGGAAGTCCTCTTCCTCCTCCTGATGTGATTCAGATTCTGTTGCAAAATCCCGAGACTGAAACCGTTATGACTCCGGATTCACCGTCTTTCCGAGGATACGTGGCAAACGGAGAAGGTGATTCTGAGACCGGTGACATGAGTAAGGTGAGACAAGACGCTTTTCTTCTGTTCAAGAACTTGTGTAAATTGTCCATGAGGTTTTCGTCCAAGGAGAACAATGATGATCAGATCATGGTGAGAGGTAAGACTCTGTCTTTGGAGTTGCTTAAAGTCATTATTGACAATGGTGGTTCTGTTTGGCGGACTAATGAAAGCTTCATCAATGCCGTTAAGCAATACTTATGCTTATCACTACTAAAGAACAGTGCGGTATCAATCATGTCAATATTTCAATTGCAATGTGCTATCTTCATGAGCTTGTTATCAAAGCTTAGATCAGTCTTGAAAGCAGAAATCGGCATTTTCTTTCCGATGATTGTTCTTCGCGTGCTTGAGAATGTTCTTCAACCTAGTTACTTGCAGAAGATGACTGTTCTCAACTTGCTAGACAAAATGTCGCAAGATCCGCAGTTGATGGTCGATATCTTTGTGAACTATGACTGCGATGTGGAGTCTTCAAACATACTTGAAAGGATTGTCAACGGTCTTCTCAAAACTGCTCTAGGACCACCCACCGGATCATCAACAACGTTGTCTCCAGCGCAAGATAGTACATTCAGGAATGATTCTGTCAAGTGTTTGGTTAACTTAGCCAAGGCAATGGGAAATTGGATGGACCAGCAGTTGAAAGTGAACGAGACGGTCTGGCCTAAGGGCTCACAAGTTTATGCTTCTATGGACAGTAATGCAAGCCAGATTAGTGAGTTAGAAGGAACTATATCTGACTGTGATTCACAGCCAGATACTAGTAATCCAGAAGCTTATGATGCTTCTATGCTTGAGCAACGAAGGGCTTACAAAATTGAATTGCAGAAAGGTATCTCTCTGTTCAATAGGAAGCCGTCGAAAGGTGTCGAATTTCTCATAAGTACCAAGAAAATAGGTAGCTCCCCTGAGGAAGTTGCTTCGTTTCTGATGAAGACCGCAGGGCTAAATGGAACTGTGATTGGTGATTATCTTGGTGAAAGAGATGAACTTCCACTAAAAGTTATGCATGCATATGTTGATTCGTTTAACTTTGAAAAGAAGGATTTTGTCGAAGCAATACGGTTCTTTCTACGTGGGTTTAGGCTACCAGGAGAAGCACAGAAGATTGATCGAATCATGGAGAAGTTTGCAGAACATTACTGGAAATGCAACCCTGGTTCTTTTACTAGTGCAGACACTGCTTATGTTCTTGCATACTCTGTGATAATGCTCAACACAGATGCACACAACAACATGGTTAAGGATAAGATGACGAAAGCTGACTTCGTCCGCAACAATAGGGGGATAGACGATGGAAAGGACTTGCCTGAAGAGTATTTAGGTTCTCTATATGATCGAGTTGTGAAAGAAGAAATCAGGATGAACTCGGACACTTTAGCCCCACAAAACAAACAGGTGAATGGCTTGAATAAACTGTTGGGCCTAGACGGAATTCTCAATCTGGTTTCTTGGATGCAACCAGATGAGAAACCTCATGGTGCCAATGGACGTCTTATAAGGGATATTCAGGAACAGTTCCAAGCTAAGCCAGAGAAATCAGAGTCAGTTTATCATACTGTTACAGATATTTCGATCTTGAGATTCATACTAGAGGTCTCTTGGGGACCGATGCTTGCTGCATTCAGTGTCACTATTGACCAGAGTGATGATAGACTAGCAACTTCTCTTTGCTTACAAGGCTTCAGATATGCGGTTCATGTCACTGCAGTTATGGGAATGCAAACTCAAAGAGATGCTTTTGTAACTTCCATGGCCAAGTTCACTAATCTTCATTGTGCTGCAGATATGAAGCAGAAAAATGTTGATGCTGTTAAAGCGATAATAACAATAGCCATAGAAGACGGTAACCATCTACATGGTTCTTGGGAACATATTTTAACTTGTCTTTCACGCATTGAACATTTGCAACTCCTTGGAGAAGTATCACCATCAGAGAAACGTTACGTCCCTACAAAAAAAGCTGAGGTAGATGACAAGAAAGCTTTAGGGTTTCCAAATTTAAAGAAACGTGGATCTTTTCAGAATCCATCTGTTATGGCTGTTGTTAGGGGAGGTTCTTATGATAGTACTTCACTTGTGAAAAGTGTTCCCAAACTAGTTACTCCGGAGCAGATCAAAAGTTTCATTGCAAATTTGAATCTACTTGATCAAATTGGCAATTTTGAGTTGAACCATGTCTATGCTAATAGCCAAAGGTTGAACAGTGAAGCTATAGTTTCTTTTGTTAAAGCGCTATGTAAAGTCTCCATGTCAGAACTTCAGTCACCAACAGATCCTCGTGTCTTTAGCCTCACGAAACTAGTCGAAACAGCGCATTATAACATGAACCGGATCCGATTAGTTTGGTCCCGCATATGGAATGTTCTTTCTGATTTCTTCGTATCAGTTGGCTTGTCAGAGAATCTCTCTGTTGCAATATTTGTCATGGACTCGCTTCGTCAGCTTTCCATGAAATTCTTGGAGCGTGAGGAGCTAGCAAACTACCATTTCCAACATGAGTTTCTTCGACCATTCGTCGTTGTTATGCAGAAAAGCAGTTCCGCGGAGATTAGAGAGCTAATAGTCCGATGTGTTTCTCAAATGGTTCTTAGTCGAGTTAGTAATGTGAAATCTGGTTGGAAAAATGTCTTCACAGTTTTTACAACTGCAGCGCTTGATGAGAGGAAGAACATTGTACTGTTAGCTTTCGAGACAATCGAGAAAATTGTGCGGGATCATTTTCATTGCATAATCGAGACAGAGATAACGGTATACGCAGACTGCATTAGATGTCTCATCACCTTCACAAACAGCAAGTTTGAAGGTGACATTGGCTTCAACACCATAGAATTTCTTCGTTTCTGCGCTTTAAAGCTTGAAGAAGGAGGACTTGTCTTGAATGAGAAACTCAAGAACAATACTATCTCAGCGTTAAAGGAAGATTTTTCTGATACACAGAGTTTTACTGACCTTGATGAACAAGTATCCTATTGGATTCCTCTGCTTACAGGTTTATGTAAGCAAGTCTCGGATCCAAGACCAGCAATTCGAAAGCGTTCCATAGAAGTATTGTTCCACATTCTGATGGATCATGGTCATCTTTTTACAAGACCCTTTTGGACTGGAATCTTCAGCTCTATCATTCTCCCAGTTTTCAACAACATACGAAGTAAAACCGATATGCTATTTGAAGAAAGTGTTGATTCGCCATCCTCAGCTTCTCTAGACACCGAAGAAACTACATGGGACGTTGAGACATCAACACTTGCATTGCAGCTTCTCGTCGATCTACTCGTTAAATTTTTCAGATCCGTGAGATCTCAGCTTCCAAGCGTTGTGTCAATCATCGTAGGGTTCATAAAGAGTCCTTTTCAAGGCTCTACAGGCTCTGGAATCTCTGTTTTGCTACATCTGGCTGATGGATTGGCTCGTAGTGCCTCTGAAGATGAATGGAGAGAGATATTCTTGGCTCTCAAGGAAGCAGCGTCGCTGACTTTCGCCGGTTTCATGAAAGTCTTGAGAACAATGGATGATATCGAAGATGTTGAAACACTTTCTGGTCAAAGCGTAAACATAGGTGATCTTGATGATGACAGTCTTCACATCATGTCGTATGTTGTCTCCAGAACAAAGAAACACATTGATGTCCTGTCTCAAATCGTTGAGGTTGTGAGTGATTTATACAGAAGAAACCAGTTCTCATTGTCCGCATCACACGTGGATATTCTGGCAGACATTTTCTCGTGCATCGCGTCTCACGCACAGCAACTAAACACCGACACAGTCCTTAGAAGGAAGTTCAAGAGAGCATGCTCTGTCCAAAACCTAACAGAGCCTCAACTACTTAACTTCGAGAACGAGGCTTACAAATCCTACATGATGTTCCTCCAAGACATGGTTACTTGCAATCCAAACGTCTCCAAAGAACTAGATCTCGAGTCTCGTCTAGTCACTGAATGCGCAAAAATCGTGAAGATCTACCTTAAATGCACAGATCCACAACAACAAGAACAACAACAAAGAAAACCGGTGCTATGGGTTCTTCCGATGGAATCAGATCGTGTAGAAGAAGCAACGGCGAGAACTTCACTGCTTGTTTCTTCACTCGAAGCCTTGTGCTCATTAGAAGCAGAGTCTCTGAAGAAACACGTGTCCAGCTTCTTCCCTTTGTTGGTGGATCTTGTGAGGACTGAGCATTGCTCTCCACAAGTTCCGTACGTCCTAAGCAATGTTTTGAAATCGTGCATTGGTCCTATTTTGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1706

Amino Acids

190.82

Weight (kDa)

5.71

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 23 - 199 2.5e-20 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 293 - 451 2.4e-45 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 563 - 744 8.2e-74 Sec7 domain
Sec7-like_HDS PF09324 1110 - 1192 1.8e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1266 - 1375 1.1e-06 C-terminal region of Mon2 protein
BIG2_C PF20252 1504 - 1697 1.3e-24 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 501, 2372, 3195
AasI GACNNNNNNGTC 1 cut(s) 732
AatII GACGTC 1 cut(s) 2368
Acc16I TGCGCA 1 cut(s) 4816
AccB1I GGYRCC 2 cut(s) 26, 2354
AccB7I CCANNNNNTGG 1 cut(s) 4750
AccBSI CCGCTC 1 cut(s) 525
AccI GTMKAC 1 cut(s) 3627
AccII CGCG 4 cut(s) 315, 1215, 3419, 4607
AccIII TCCGGA 2 cut(s) 802, 2980
AclI AACGTT 2 cut(s) 1418, 2815
AcoI YGGCCR 2 cut(s) 40, 2628
AcsI RAATTY 7 cut(s) 1745, 2307, 2872, 3007, 3329, 3704, 4154
AcuI CTGAAG 7 cut(s) 612, 2205, 2542, 3119, 3970, 4317, 5015
AcvI CACGTG 2 cut(s) 4573, 5005
AcyI GRCGYC 2 cut(s) 27, 2365
AdeI CACNNNGTG 1 cut(s) 38
AfaI GTAC 6 cut(s) 104, 1441, 1759, 2944, 3552, 5072
AfeI AGCGCT 2 cut(s) 3108, 3528
AfiI CCNNNNNNNGG 6 cut(s) 1403, 1736, 3231, 4229, 4750, 5026
AflIII ACRYGT 6 cut(s) 32, 99, 481, 4570, 5002, 5004
AgeI ACCGGT 2 cut(s) 857, 4882
AhdI GACNNNNNGTC 2 cut(s) 1282, 3389
AhlI ACTAGT 4 cut(s) 1634, 2042, 2971, 3175
AjnI CCWGG 2 cut(s) 1963, 2029
AjuI GAANNNNNNNTTGG 6 cut(s) 2276, 2308, 3360, 3392, 3669, 3701
Alw21I GWGCWC 1 cut(s) 4976
AlwNI CAGNNNCTG 3 cut(s) 766, 2054, 4244
Ama87I CYCGRG 2 cut(s) 779, 4790
Aor13HI TCCGGA 2 cut(s) 802, 2980
Aor51HI AGCGCT 2 cut(s) 3108, 3528
AoxI GGCC 5 cut(s) 40, 187, 1535, 2296, 2628
ApeKI GCWGC 8 cut(s) 83, 434, 719, 2492, 2654, 3524, 4129, 4337
ApoI RAATTY 7 cut(s) 1745, 2307, 2872, 3007, 3329, 3704, 4154
ArsI GACNNNNNNTTYG 4 cut(s) 967, 999, 3885, 3917
AsiGI ACCGGT 2 cut(s) 857, 4882
Asp700I GAANNNNTTC 4 cut(s) 147, 402, 3708, 3910
AspLEI GCGC 8 cut(s) 29, 284, 1432, 3109, 3190, 3529, 3722, 4817
AspS9I GGNCC 7 cut(s) 53, 1394, 1504, 2296, 2480, 3222, 5105
AsuII TTCGAA 1 cut(s) 3904
AsuNHI GCTAGC 1 cut(s) 3349
AvaI CYCGRG 2 cut(s) 779, 4790
AvaII GGWCC 6 cut(s) 53, 1394, 1504, 2480, 3222, 5105
AxyI CCTNAGG 2 cut(s) 1539, 1782
BaeI ACNNNNGTAYC 2 cut(s) 86, 119
BalI TGGCCA 1 cut(s) 2630
BamHI GGATCC 2 cut(s) 3207, 3886
BanI GGYRCC 2 cut(s) 26, 2354
BanII GRGCYC 1 cut(s) 1547
BauI CACGAG 2 cut(s) 3153, 4597
BbrPI CACGTG 2 cut(s) 4573, 5005
BbsI GAAGAC 6 cut(s) 1335, 1367, 1814, 2721, 3496, 4445
Bbv12I GWGCWC 1 cut(s) 4976
BbvCI CCTCAGC 2 cut(s) 2838, 4069
BbvI GCAGC 8 cut(s) 70, 421, 706, 2479, 2641, 3536, 4141, 4349
BceAI ACGGC 5 cut(s) 353, 422, 1040, 1714, 4947
BciT130I CCWGG 2 cut(s) 1965, 2031
BciVI GTATCC 2 cut(s) 818, 3850
BclI TGATCA 2 cut(s) 952, 3022
BcuI ACTAGT 4 cut(s) 1634, 2042, 2971, 3175
BfmI CTRYAG 7 cut(s) 387, 720, 2580, 2655, 3087, 3522, 4236
BfoI RGCGCY 3 cut(s) 30, 3110, 3530
BfuI GTATCC 2 cut(s) 818, 3850
BglII AGATCT 6 cut(s) 443, 537, 587, 4172, 4786, 4830
BlpI GCTNAGC 1 cut(s) 2400
BmcAI AGTACT 1 cut(s) 2944
Bme1390I CCNGG 2 cut(s) 1965, 2031
Bme18I GGWCC 6 cut(s) 53, 1394, 1504, 2480, 3222, 5105
BmeRI GACNNNNNGTC 2 cut(s) 1282, 3389
BmeT110I CYCGRG 2 cut(s) 779, 4790
BmgT120I GGNCC 7 cut(s) 53, 1394, 1504, 2296, 2480, 3222, 5105
BmiI GGNNCC 6 cut(s) 28, 2356, 2481, 3209, 3224, 3888
BmrFI CCNGG 2 cut(s) 1965, 2031
BmrI ACTGGG 1 cut(s) 3998
BmtI GCTAGC 1 cut(s) 3353
BmuI ACTGGG 1 cut(s) 3998
BpiI GAAGAC 6 cut(s) 1335, 1367, 1814, 2721, 3496, 4445
BplI GAGNNNNNCTC 6 cut(s) 414, 446, 3266, 3298, 5039, 5071
BpmI CTGGAG 2 cut(s) 1410, 4462
Bpu10I CCTNAGC 3 cut(s) 2838, 4069, 5078
Bpu1102I GCTNAGC 1 cut(s) 2400
Bpu14I TTCGAA 1 cut(s) 3904
BpuEI CTTGAG 7 cut(s) 541, 1241, 1688, 2473, 3749, 4313, 4393
BsaAI YACGTR 5 cut(s) 102, 829, 1949, 4573, 5005
BsaHI GRCGYC 2 cut(s) 27, 2365
BsaI GGTCTC 2 cut(s) 848, 2476
BsaWI WCCGGW 6 cut(s) 802, 857, 1403, 2980, 3204, 4882
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 1408, 1438, 3732, 3762
Bsc4I CCNNNNNNNGG 6 cut(s) 1403, 1736, 3231, 4229, 4750, 5026
Bse118I RCCGGY 3 cut(s) 857, 4355, 4882
Bse1I ACTGG 4 cut(s) 2021, 3982, 4004, 4549
Bse21I CCTNAGG 2 cut(s) 1539, 1782
Bse3DI GCAATG 7 cut(s) 1133, 1494, 3000, 3598, 4124, 5050, 5089
BseAI TCCGGA 2 cut(s) 802, 2980
BseBI CCWGG 2 cut(s) 1965, 2031
BseGI GGATG 5 cut(s) 1506, 2241, 2334, 4064, 4390
BseLI CCNNNNNNNGG 6 cut(s) 1403, 1736, 3231, 4229, 4750, 5026
BseMI GCAATG 7 cut(s) 1133, 1494, 3000, 3598, 4124, 5050, 5089
BseMII CTCAG 8 cut(s) 575, 843, 1773, 2829, 3795, 4083, 4190, 5037
BseNI ACTGG 4 cut(s) 2021, 3982, 4004, 4549
BseRI GAGGAG 2 cut(s) 735, 3359
BseXI GCAGC 8 cut(s) 70, 421, 706, 2479, 2641, 3536, 4141, 4349
BsgI GTGCAG 1 cut(s) 2067
Bsh1236I CGCG 4 cut(s) 315, 1215, 3419, 4607
BshFI GGCC 5 cut(s) 42, 189, 1537, 2298, 2630
BshNI GGYRCC 2 cut(s) 26, 2354
BshTI ACCGGT 2 cut(s) 857, 4882
BsiHKAI GWGCWC 1 cut(s) 4976
BsiHKCI CYCGRG 2 cut(s) 779, 4790
BsiSI CCGG 8 cut(s) 43, 803, 858, 1404, 2981, 3205, 4356, 4883
BsiWI CGTACG 1 cut(s) 5070
BslFI GGGAC 4 cut(s) 2493, 2807, 3208, 4115
BslI CCNNNNNNNGG 6 cut(s) 1403, 1736, 3231, 4229, 4750, 5026
BsmBI CGTCTC 5 cut(s) 187, 376, 1520, 4614, 4777
BsmFI GGGAC 4 cut(s) 2493, 2807, 3208, 4115
BsmI GAATGC 3 cut(s) 2495, 2598, 4817
BsnI GGCC 5 cut(s) 42, 189, 1537, 2298, 2630
Bso31I GGTCTC 2 cut(s) 848, 2476
BsoBI CYCGRG 2 cut(s) 779, 4790
Bsp119I TTCGAA 1 cut(s) 3904
Bsp1286I GDGCHC 2 cut(s) 1547, 4976
Bsp13I TCCGGA 2 cut(s) 802, 2980
Bsp1720I GCTNAGC 1 cut(s) 2400
Bsp19I CCATGG 1 cut(s) 2625
BspANI GGCC 5 cut(s) 42, 189, 1537, 2298, 2630
BspCNI CTCAG 8 cut(s) 574, 844, 1774, 2830, 3794, 4082, 4189, 5038
BspEI TCCGGA 2 cut(s) 802, 2980
BspFNI CGCG 4 cut(s) 315, 1215, 3419, 4607
BspHI TCATGA 2 cut(s) 1140, 4362
BspLI GGNNCC 6 cut(s) 28, 2356, 2481, 3209, 3224, 3888
BspMAI CTGCAG 4 cut(s) 724, 2584, 2659, 3526
BspOI GCTAGC 1 cut(s) 3353
BspT104I TTCGAA 1 cut(s) 3904
BspT107I GGYRCC 2 cut(s) 26, 2354
BspTNI GGTCTC 2 cut(s) 848, 2476
BsrBI CCGCTC 1 cut(s) 525
BsrDI GCAATG 7 cut(s) 1133, 1494, 3000, 3598, 4124, 5050, 5089
BsrFI RCCGGY 3 cut(s) 857, 4355, 4882
BsrI ACTGG 4 cut(s) 2021, 3982, 4004, 4549
BssAI RCCGGY 3 cut(s) 857, 4355, 4882
BssNAI GTATAC 1 cut(s) 3628
BssNI GRCGYC 2 cut(s) 27, 2365
BssSI CACGAG 2 cut(s) 3153, 4597
BssT1I CCWWGG 5 cut(s) 511, 936, 1482, 2625, 2788
Bst1107I GTATAC 1 cut(s) 3628
Bst2BI CACGAG 2 cut(s) 3153, 4597
Bst2UI CCWGG 2 cut(s) 1965, 2031
Bst6I CTCTTC 6 cut(s) 118, 153, 299, 356, 744, 2181
BstACI GRCGYC 2 cut(s) 27, 2365
BstAPI GCANNNNNTGC 2 cut(s) 203, 2054
BstBAI YACGTR 5 cut(s) 102, 829, 1949, 4573, 5005
BstBI TTCGAA 1 cut(s) 3904
BstC8I GCNNGC 6 cut(s) 1217, 1579, 1884, 2490, 3351, 4666
BstDSI CCRYGG 4 cut(s) 313, 463, 2625, 3417
BstEII GGTNACC 1 cut(s) 2720
BstENI CCTNNNNNAGG 1 cut(s) 4227
BstF5I GGATG 5 cut(s) 1506, 2241, 2334, 4064, 4390
BstFNI CGCG 4 cut(s) 315, 1215, 3419, 4607
BstH2I RGCGCY 3 cut(s) 30, 3110, 3530
BstHHI GCGC 8 cut(s) 29, 284, 1432, 3109, 3190, 3529, 3722, 4817
BstNI CCWGG 2 cut(s) 1965, 2031
BstNSI RCATGY 2 cut(s) 1886, 4668
BstPI GGTNACC 1 cut(s) 2720
BstSCI CCNGG 2 cut(s) 1963, 2029
BstSFI CTRYAG 7 cut(s) 387, 720, 2580, 2655, 3087, 3522, 4236
BstUI CGCG 4 cut(s) 315, 1215, 3419, 4607
BstV1I GCAGC 8 cut(s) 70, 421, 706, 2479, 2641, 3536, 4141, 4349
BstV2I GAAGAC 6 cut(s) 1335, 1367, 1814, 2721, 3496, 4445
BstXI CCANNNNNNTGG 2 cut(s) 1489, 2732
BstZ17I GTATAC 1 cut(s) 3628
Bsu36I CCTNAGG 2 cut(s) 1539, 1782
BsuI GTATCC 2 cut(s) 818, 3850
BsuRI GGCC 5 cut(s) 42, 189, 1537, 2298, 2630
BtgI CCRYGG 4 cut(s) 313, 463, 2625, 3417
BtgZI GCGATG 2 cut(s) 1347, 4588
BtsCI GGATG 5 cut(s) 1506, 2241, 2334, 4064, 4390
BtsI GCAGTG 3 cut(s) 2052, 2577, 4943
BtsIMutI CAGTG 7 cut(s) 1099, 2052, 2506, 2577, 3085, 4806, 4943
Cac8I GCNNGC 6 cut(s) 1217, 1579, 1884, 2490, 3351, 4666
CaiI CAGNNNCTG 3 cut(s) 766, 2054, 4244
CciI TCATGA 2 cut(s) 1140, 4362
CfoI GCGC 8 cut(s) 29, 284, 1432, 3109, 3190, 3529, 3722, 4817
Cfr10I RCCGGY 3 cut(s) 857, 4355, 4882
Cfr13I GGNCC 7 cut(s) 53, 1394, 1504, 2296, 2480, 3222, 5105
Cfr42I CCGCGG 2 cut(s) 316, 3420
CseI GACGC 3 cut(s) 893, 4329, 4596
Csp6I GTAC 6 cut(s) 103, 1440, 1758, 2943, 3551, 5071
CspAI ACCGGT 2 cut(s) 857, 4882
CviQI GTAC 6 cut(s) 103, 1440, 1758, 2943, 3551, 5071
DinI GGCGCC 1 cut(s) 28
DraI TTTAAA 3 cut(s) 289, 2877, 3726
DraIII CACNNNGTG 1 cut(s) 38
DrdI GACNNNNNNGTC 1 cut(s) 732
DriI GACNNNNNGTC 2 cut(s) 1282, 3389
DseDI GACNNNNNNGTC 1 cut(s) 732
EaeI YGGCCR 2 cut(s) 40, 2628
Eam1104I CTCTTC 6 cut(s) 118, 153, 299, 356, 744, 2181
Eam1105I GACNNNNNGTC 2 cut(s) 1282, 3389
EarI CTCTTC 6 cut(s) 118, 153, 299, 356, 744, 2181
EciI GGCGGA 1 cut(s) 1045
Eco130I CCWWGG 5 cut(s) 511, 936, 1482, 2625, 2788
Eco24I GRGCYC 1 cut(s) 1547
Eco31I GGTCTC 2 cut(s) 848, 2476
Eco32I GATATC 2 cut(s) 1312, 4390
Eco47I GGWCC 6 cut(s) 53, 1394, 1504, 2480, 3222, 5105
Eco47III AGCGCT 2 cut(s) 3108, 3528
Eco57I CTGAAG 7 cut(s) 612, 2205, 2542, 3119, 3970, 4317, 5015
Eco72I CACGTG 2 cut(s) 4573, 5005
Eco81I CCTNAGG 2 cut(s) 1539, 1782
Eco88I CYCGRG 2 cut(s) 779, 4790
Eco91I GGTNACC 1 cut(s) 2720
EcoNI CCTNNNNNAGG 1 cut(s) 4227
EcoO65I GGTNACC 1 cut(s) 2720
EcoRI GAATTC 1 cut(s) 2307
EcoRII CCWGG 2 cut(s) 1963, 2029
EcoRV GATATC 2 cut(s) 1312, 4390
EcoT14I CCWWGG 5 cut(s) 511, 936, 1482, 2625, 2788
EcoT22I ATGCAT 2 cut(s) 1884, 1888
EcoT38I GRGCYC 1 cut(s) 1547
EgeI GGCGCC 1 cut(s) 28
EheI GGCGCC 1 cut(s) 28
ErhI CCWWGG 5 cut(s) 511, 936, 1482, 2625, 2788
Esp3I CGTCTC 5 cut(s) 187, 376, 1520, 4614, 4777
FalI AAGNNNNNCTT 8 cut(s) 588, 620, 1052, 1084, 2596, 2628, 3729, 3761
FaqI GGGAC 4 cut(s) 2493, 2807, 3208, 4115
FauI CCCGC 2 cut(s) 3233, 3579
FauNDI CATATG 2 cut(s) 1888, 3230
FbaI TGATCA 2 cut(s) 952, 3022
FblI GTMKAC 1 cut(s) 3627
FokI GGATG 5 cut(s) 1513, 2248, 2341, 4051, 4397
FriOI GRGCYC 1 cut(s) 1547
FspI TGCGCA 1 cut(s) 4816
GlaI GCGC 8 cut(s) 28, 283, 1431, 3108, 3189, 3528, 3721, 4816
GsuI CTGGAG 2 cut(s) 1410, 4462
HaeII RGCGCY 3 cut(s) 30, 3110, 3530
HaeIII GGCC 5 cut(s) 42, 189, 1537, 2298, 2630
HapII CCGG 8 cut(s) 43, 803, 858, 1404, 2981, 3205, 4356, 4883
HgaI GACGC 3 cut(s) 893, 4329, 4596
HhaI GCGC 8 cut(s) 29, 284, 1432, 3109, 3190, 3529, 3722, 4817
Hin1I GRCGYC 2 cut(s) 27, 2365
Hin6I GCGC 8 cut(s) 27, 282, 1430, 3107, 3188, 3527, 3720, 4815
HinP1I GCGC 8 cut(s) 27, 282, 1430, 3107, 3188, 3527, 3720, 4815
HincII GTYRAC 5 cut(s) 6, 364, 634, 1369, 1474
HindII GTYRAC 5 cut(s) 6, 364, 634, 1369, 1474
HindIII AAGCTT 5 cut(s) 1042, 1157, 1647, 2856, 3728
HpaI GTTAAC 1 cut(s) 1474
HpaII CCGG 8 cut(s) 43, 803, 858, 1404, 2981, 3205, 4356, 4883
Hpy99I CGWCG 7 cut(s) 158, 182, 266, 1735, 3395, 4142, 4345
Hsp92I GRCGYC 2 cut(s) 27, 2365
HspAI GCGC 8 cut(s) 27, 282, 1430, 3107, 3188, 3527, 3720, 4815
KasI GGCGCC 1 cut(s) 26
Kpn2I TCCGGA 2 cut(s) 802, 2980
Ksp22I TGATCA 2 cut(s) 952, 3022
KspAI GTTAAC 1 cut(s) 1474
KspI CCGCGG 2 cut(s) 316, 3420
LmnI GCTCC 4 cut(s) 1781, 2983, 3337, 3346
Lsp1109I GCAGC 8 cut(s) 70, 421, 706, 2479, 2641, 3536, 4141, 4349
MbiI CCGCTC 1 cut(s) 525
MfeI CAATTG 1 cut(s) 1121
MhlI GDGCHC 2 cut(s) 1547, 4976
MlsI TGGCCA 1 cut(s) 2630
MluNI TGGCCA 1 cut(s) 2630
Mly113I GGCGCC 1 cut(s) 27
MlyI GAGTC 8 cut(s) 793, 970, 1349, 2426, 3299, 4228, 4802, 4997
MmeI TCCRAC 3 cut(s) 244, 3391, 3473
Mox20I TGGCCA 1 cut(s) 2630
Mph1103I ATGCAT 2 cut(s) 1884, 1888
MroI TCCGGA 2 cut(s) 802, 2980
MroXI GAANNNNTTC 4 cut(s) 147, 402, 3708, 3910
MscI TGGCCA 1 cut(s) 2630
MslI CAYNNNNRTG 5 cut(s) 1887, 2730, 3054, 3938, 4497
Msp20I TGGCCA 1 cut(s) 2630
MspA1I CMGCKG 2 cut(s) 315, 3419
MspI CCGG 8 cut(s) 43, 803, 858, 1404, 2981, 3205, 4356, 4883
MspR9I CCNGG 2 cut(s) 1965, 2031
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 3 cut(s) 2495, 2598, 4817
MvaI CCWGG 2 cut(s) 1965, 2031
MvnI CGCG 4 cut(s) 315, 1215, 3419, 4607
NarI GGCGCC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 2625
NdeI CATATG 2 cut(s) 1888, 3230
NheI GCTAGC 1 cut(s) 3349
NlaIV GGNNCC 6 cut(s) 28, 2356, 2481, 3209, 3224, 3888
NmuCI GTSAC 7 cut(s) 9, 860, 2503, 2575, 3138, 3680, 4804
NsbI TGCGCA 1 cut(s) 4816
NsiI ATGCAT 2 cut(s) 1884, 1888
NspI RCATGY 2 cut(s) 1886, 4668
NspV TTCGAA 1 cut(s) 3904
PaeI GCATGC 2 cut(s) 1886, 4668
PaeR7I CTCGAG 1 cut(s) 4790
PagI TCATGA 2 cut(s) 1140, 4362
PcsI WCGNNNNNNNCGW 6 cut(s) 138, 162, 366, 3570, 4146, 4604
PctI GAATGC 3 cut(s) 2495, 2598, 4817
PdmI GAANNNNTTC 4 cut(s) 147, 402, 3708, 3910
Pfl23II CGTACG 1 cut(s) 5070
PflFI GACNNNGTC 3 cut(s) 979, 2141, 4637
PflMI CCANNNNNTGG 1 cut(s) 4750
PinAI ACCGGT 2 cut(s) 857, 4882
PleI GAGTC 8 cut(s) 793, 970, 1348, 2425, 3299, 4227, 4801, 4996
PluTI GGCGCC 1 cut(s) 30
PmaCI CACGTG 2 cut(s) 4573, 5005
PmlI CACGTG 2 cut(s) 4573, 5005
PpsI GAGTC 8 cut(s) 793, 970, 1348, 2425, 3299, 4227, 4801, 4996
Ppu21I YACGTR 5 cut(s) 102, 829, 1949, 4573, 5005
PsiI TTATAA 3 cut(s) 501, 2372, 3195
Psp1406I AACGTT 2 cut(s) 1418, 2815
Psp6I CCWGG 2 cut(s) 1963, 2029
PspCI CACGTG 2 cut(s) 4573, 5005
PspEI GGTNACC 1 cut(s) 2720
PspGI CCWGG 2 cut(s) 1963, 2029
PspLI CGTACG 1 cut(s) 5070
PspN4I GGNNCC 6 cut(s) 28, 2356, 2481, 3209, 3224, 3888
PspPI GGNCC 7 cut(s) 53, 1394, 1504, 2296, 2480, 3222, 5105
PsrI GAACNNNNNNTAC 2 cut(s) 4703, 4735
PstI CTGCAG 4 cut(s) 724, 2584, 2659, 3526
PstNI CAGNNNCTG 3 cut(s) 766, 2054, 4244
PsyI GACNNNGTC 3 cut(s) 979, 2141, 4637
RsaI GTAC 6 cut(s) 104, 1441, 1759, 2944, 3552, 5072
RsaNI GTAC 6 cut(s) 103, 1440, 1758, 2943, 3551, 5071
RseI CAYNNNNRTG 5 cut(s) 1887, 2730, 3054, 3938, 4497
SacII CCGCGG 2 cut(s) 316, 3420
Sau96I GGNCC 7 cut(s) 53, 1394, 1504, 2296, 2480, 3222, 5105
ScaI AGTACT 1 cut(s) 2944
SchI GAGTC 8 cut(s) 793, 970, 1349, 2426, 3299, 4228, 4802, 4997
ScrFI CCNGG 2 cut(s) 1965, 2031
SduI GDGCHC 2 cut(s) 1547, 4976
SfcI CTRYAG 7 cut(s) 387, 720, 2580, 2655, 3087, 3522, 4236
SfoI GGCGCC 1 cut(s) 28
Sfr274I CTCGAG 1 cut(s) 4790
Sfr303I CCGCGG 2 cut(s) 316, 3420
SfuI TTCGAA 1 cut(s) 3904
SgrBI CCGCGG 2 cut(s) 316, 3420
SinI GGWCC 6 cut(s) 53, 1394, 1504, 2480, 3222, 5105
SlaI CTCGAG 1 cut(s) 4790
SmiMI CAYNNNNRTG 5 cut(s) 1887, 2730, 3054, 3938, 4497
SmlI CTYRAG 8 cut(s) 520, 1220, 1667, 2452, 3764, 4328, 4372, 4790
SmoI CTYRAG 8 cut(s) 520, 1220, 1667, 2452, 3764, 4328, 4372, 4790
SpeI ACTAGT 4 cut(s) 1634, 2042, 2971, 3175
SphI GCATGC 2 cut(s) 1886, 4668
SspDI GGCGCC 1 cut(s) 26
SspI AATATT 3 cut(s) 505, 1116, 3294
StyD4I CCNGG 2 cut(s) 1963, 2029
StyI CCWWGG 5 cut(s) 511, 936, 1482, 2625, 2788
TaqII GACCGA 1 cut(s) 2497
TatI WGTACW 3 cut(s) 1439, 2942, 3550
TauI GCSGC 3 cut(s) 27, 197, 528
TscAI CASTG 7 cut(s) 1099, 2059, 2506, 2584, 3085, 4813, 4950
TseFI GTSAC 7 cut(s) 9, 860, 2503, 2575, 3138, 3680, 4804
TseI GCWGC 8 cut(s) 83, 434, 719, 2492, 2654, 3524, 4129, 4337
Tsp45I GTSAC 7 cut(s) 9, 860, 2503, 2575, 3138, 3680, 4804
TspGWI ACGGA 9 cut(s) 115, 166, 202, 452, 668, 852, 2319, 4156, 5058
TspRI CASTG 7 cut(s) 1099, 2059, 2506, 2584, 3085, 4813, 4950
Tth111I GACNNNGTC 3 cut(s) 979, 2141, 4637
Van91I CCANNNNNTGG 1 cut(s) 4750
VpaK11BI GGWCC 6 cut(s) 53, 1394, 1504, 2480, 3222, 5105
XagI CCTNNNNNAGG 1 cut(s) 4227
XapI RAATTY 7 cut(s) 1745, 2307, 2872, 3007, 3329, 3704, 4154
XbaI TCTAGA 2 cut(s) 274, 4078
XceI RCATGY 2 cut(s) 1886, 4668
XcmI CCANNNNNNNNNTGG 1 cut(s) 3331
XhoI CTCGAG 1 cut(s) 4790
XmiI GTMKAC 1 cut(s) 3627
XmnI GAANNNNTTC 4 cut(s) 147, 402, 3708, 3910
ZraI GACGTC 1 cut(s) 2366
ZrmI AGTACT 1 cut(s) 2944
Zsp2I ATGCAT 2 cut(s) 1884, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.