RchiOBHm_Chr6g0281241

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002933
Physical Location & Seq
Forward (+)
44573087 .. 44583617
10531 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25224

Sequence Viewer

Length: 4593 bp
ATGGTCATCGTCTTTACCAGAGTGGAGGCCGATGCTATGTACGTCACCATCTCCAGGGTTTCGGTCAGCGAATTGCTGGAATTTACTGATAAGAATCTGAATGAAGGGAGCTCCATTATGTTCTGCCAGAATTTCATCAATGAGGTTATGGAAGCTAGTTATGGGGGTCCTGATAGTATCAAAATGGCGGCTCCGGCCCCCAGGAGATTGCAAAATGGAAATGCCGCCGCTGGACGCGGTGAGTCGGGGGCTGGAGAGTCCAATGATGAAGCTGAATCTGGCGAGGGAGGTGGTAGTAGTAAGATTCGGGATGATGGATTTCTTTTGTTTAAGAATTTGTGTAAGTTGTCGATGAAGTTCTCGTCACAGGAGTACTCTGATGATCAAATACTATTGAGGGGTAAAATATTGTCGTTGGAGCTTCTGAAGGTGGTTATGGACAATGGGGGTCCTATTTGGCTCTCTAATGACAGGTTTCTCAATGGCATCAAGCAGTTCCTATGCTTATCATTGCTAAAAAACAGTGCGCTGTCAGTTATGGCTATTTTCCAGCTCCAGTGTTCTATTTTCACGAGCTTGTTATCAAAATTTAGATCAGGGTTGAAAGCAGAAATCGGTATATTTTTTCCCATGCTCGTCTTACGCGTGCTTGAGAATGTTCTCCAGCCTAGTTTCTTGCAGAAAATGACGGTCCTCAATTTATTAGAGAAGATCTCTCAGGATCCACAGATTATCATTGACATTTTTGTCAACTATGACTGTGATGTGGATTCCCCAAACATTTTCGAAAGGATTGTCAACGGCCTTCTGAAAACTGCTCTAGGTCCACCTTCTGGTTCAACAACAACATTATCTCCGGTTCAGGATATTACTTTCAGGCATGAATCAGTGAAGTGCTTGGTTAACATCATTAAGTCAATGGGAGCTTGGATGGACCGACAGAGACTGGGAGACTCCTATCTACCAAATACCAATGAGAGTGACACTACATCAGAGAAAACAGAGAATCAATTGACCCTAAATGGTGAAGAAGGAACCGCTTTAGACAATGAATTACATCCAGAAGGAAGTTCAGATGCTGCAACTCTCGAGCAACGTCGAGCTTTTAAACTTGAACTGCAGAAAGGTATCTCACTGTTCAATAGGAAACCTTCCAAGGGCATTGAGTTTCTGATAAGCACAAAAAAAATTGGGGGTTCTCCAGCAGATGTGGCTTCTTTCCTGAAGAACAACACTGCGGGCCTAAATGAAACCATGATCGGTGACTATTTGGGTGAAAGGGAAGAATTTTGTCTAAAAGTTATGCATGCTTATGTAGATTCCTTTAATTTCAAAGGGATGGATTTTGGTGAAGCAATTAGGTTTTTCCTGCGGGGGTTCAAGTTACCTGGAGAAGCACAGAAAATTGACCGAATCATGGAGAAGTTTGCTGAGCGTTATTGTAAATGCAGTCCGAATTCATTTACTAGTGCAGATACTGCTTATGTCCTTGCATACTCTGTGATAATGCTCAACACGGATGCCCATAATATCATGGTTAAAGATAAGATGACCAAGGCCGATTTCATACGTAACAACCGAGGAATAGATGATGGCAAGGATCTACCTGAGGAATATCTTGGTGCACTATATGATCAGATTGTTAAAAATGAAATTAAGATGAAGGCTGACTCTTCTGTACCACAGAGCAAGCAGGAAAACAGCTTTAACAAATTATTGGGCCTGGATGGTATCCTGAATCTAGTAACTGGGAAGCAGACTGAAGAAAAAGCGTTGGGTGCAAATGGGCTTCTTATAAAGGACATCCAAGAGCAATTTAAGGCGAAGTCAGGGAAATCAGAGTCTGTTTATCATTCTGTGACAGATGTGGCCATATTGAGGTTCATGGTGGAGGCCTGCTGGGGTCCTATGCTGGCCGCATTCAGTGTGACTCTGGACCAAAGTGATGACAGGCTTGCTACTGCTCAATGCTTACTGGGTTTCCGACATGCAATTCATGTTACTGCTCTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACATCAATGGCAAAGTTTACTTATCTACATAATGCTGCAGATATGCGGCAAAAGAATGTTGACGCTGTGAAGGCAATAATAGCAATCGCCGTTGAAGATGGTAATCATCTTCAGGAAGCATGGGAGCACATATTAACATGCCTTTCCCGAATTGAGCATCTGCAACTGTTGGGTGAGGGCGCACCTACTGATGCAACCTTTTTCAGTGGATCTAATGTTGAAGCGGATGACAAATCACCAAAGCCTATTGGTTTTGCTTCTCTAAAGAAAAAAGGAACTATCCAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGATAGCACTAGTATTGGAATCAACACTTCGGGACTGGTATCTCCAGAACAGATTAATAATTTTATTTCGAATTTGAATTTGCTGGATCAGATTGGGAATTTTGAGTTGAACCATGTATTTGCTCATAGCCAAAGCTTGAACAGTGAAGCCATAGTTGCTTTTGTGAAGTCCTTATGTAAAGTTTCCATGTCAGAATTGCAGTCTCCAACAGATCCTCGTGTTTTCAGCCTCACAAAAATAGTTGAAATAGCGCATTATAATATGAACCGCATCAGATTAGTGTGGTCTCGAATATGGAATGTACTGTCAGATTTCTTTGTATCAGTTGGCTTGTCAGAGAATCTTTCAGTTGCAATATTTGTGATGGATTCCTTGCGTCAGCTTGCTATGAAATTCTTAGAGCGTGAGGAGCTGGCAAATTACAATTTCCAGAATGAATTTTTAAGACCATTTGTGATTGTTATGCAGAAAAGCAGCTCTACAGAAATTAGGGAATTAATAGTTCGGTGCATTTCACAAATGGTCCTCAGCCGGGTCAATAACGTGAAATCTGGCTGGAAAAGTGTTTTTATGGTTTTCACAATTGCTGCTGCTGATGAGAGGAAAAATATAGTCTTGTTGGCCTTTGAGACCATGGAAAAGATAGTCAGAGAGTATTTCCCTTACATAACTGAGACAGAGACATTGACTTTTACTGATTGTGTTAGATGCCTCTTAACCTTCACAAATAGCAGATTCAATAGTGATGTTAGCCTCAATGCTATTGCATTTCTGCGTTTCTGTGCCGTCAAACTTGCAGAAGGAGGCCTTGTTTACAATAAGAACAGTGAGATTGGTGGTTCTTCAAGTCCTAGCGCAAGTGAAGGTGCTTCAACTGTTGAGAATTTCAATGATAAAGATGATCATACATCATTTTGGGTTCCATTGCTAACAGGTTTGTCCAAACTAACATCTGATCCTAGATCAGCTATCAGGAAAGGATCCTTGGAAGTTCTTTTCAACATTCTAAAGGACCATGGTCATCTTTTCTCACACCTGTTTTGGACTGCGGTTTTCAATTCTGTTATTTTCCCCATATTTAATGGTGTCAGCGATAAGAAAGATACAGACATGAAAAATGACCAGTCCTCGCCAATTTCGATGTCCCCACGTCCTGAAGGAAGTACATGGGATTCTGAAACTTCAGCTGTTGCGGCGGATTGCCTCATAGATCTGTTCGTCAGTTTCTTTGATGTTGTTAGGTCTCAACTACCAAGTGTGATATCCATTCTGACAGGACTCATCAGAAGTCCCATTCAGGGTCCTGCTACTGCAGGAGTTGCTGCATTAATGCGTTTGTCTAGTGAGGTGGGTGGAAGGCTTTCAGAAGATGAATGGAGAGCAATTTTTCTGGCTTTAAAGGAGGCTGCCACATCTGCAGTGCCTGGATTTTTGAAGGTCTTGAGAACCATGGATAATGTCAATGTTCCTGGTCTTTCTGAATCTTTTAGTGATATCGATATGTCGTCTGATCAGGGGTACTCAAATGAGGATCTTGAGGATGATAATTTGCAAACAGCTTCATATGTGGTTTCAAGAATCAAGAGTCATGTTTCTATGCAGCTACTCATCTTACAGGTCGCTGCTGATTTGTACAAAATAAACCTAGAAACTTTGTCAGCTGCTAACATTGCAGTTCTTCTTGAAGTATATTCTCTTGTGGCATCGCACGCTCACCAACTGAACTCTGAGACAATTCTGCAGAAGAAGCTGGAAAAAGTGTGTTCCATCCTAGAACTCACTGCACCACCTATTGTTCATTTTGAGAATGAATCTTACAAAAACTATCTTAACTTCCTCCAAAACGCACTTGTGGATAATCCATCTCTTTCCAAGGAAATGGACATAGAAGCTAAACTTGTGGCAGTCTGTGAAAACATATTGCAGATATACCTAAAGTGTACTGAGCTTCAATCTTCTGAGCAGAAGCCTACCGGTCAGCCAGTTTTGCACTGGATTCTTCCTTTGGGTACAGCAAAAAAGGAGGAATTGGCTACCAGGACTTTCTTAGCTGTCTCTTCATTGCAGGCATTGAGTGGTTTGGAAAAGGTTTCATTTAGGAGGCACGTTTCTCAGTTATTTCCTCTGTTGGTAGATCTTGTTAAGAGTGAGCACACTTCTGGAGAAGTTCAACATGTTCTAAGCAACATATTCCAGTCATGTATAGGCCCAACAATAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1530

Amino Acids

169.42

Weight (kDa)

5.38

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 103 - 261 2.3e-42 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 370 - 552 9.6e-75 Sec7 domain
Sec7-like_HDS PF09324 920 - 1002 2.2e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1093 - 1158 5.2e-06 C-terminal region of Mon2 protein
BIG2_C PF20252 1322 - 1521 2.7e-26 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1796, 2625
AasI GACNNNNNNGTC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 833
AccII CGCG 2 cut(s) 237, 645
AcoI YGGCCR 2 cut(s) 1869, 1914
AcuI CTGAAG 6 cut(s) 446, 1244, 1782, 2138, 3534, 3543
AfaI GTAC 9 cut(s) 41, 374, 1680, 2670, 3532, 3887, 4001, 4309, 4378
AfiI CCNNNNNNNGG 7 cut(s) 54, 833, 1157, 1417, 1878, 2899, 3665
AflIII ACRYGT 2 cut(s) 643, 4540
AgeI ACCGGT 1 cut(s) 4340
AhlI ACTAGT 2 cut(s) 1466, 2375
AjiI CACGTC 1 cut(s) 3518
AjnI CCWGG 7 cut(s) 53, 200, 1387, 1722, 3790, 3835, 4403
AjuI GAANNNNNNNTTGG 4 cut(s) 1547, 1579, 3335, 3367
Alw21I GWGCWC 4 cut(s) 113, 1627, 2172, 4521
Alw44I GTGCAC 1 cut(s) 1623
AlwNI CAGNNNCTG 5 cut(s) 946, 1079, 1478, 1844, 3791
Ama87I CYCGRG 1 cut(s) 1088
ApaLI GTGCAC 1 cut(s) 1623
ArsI GACNNNNNNTTYG 2 cut(s) 780, 812
AseI ATTAAT 3 cut(s) 2421, 2864, 3695
AsiGI ACCGGT 1 cut(s) 4340
Asp700I GAANNNNTTC 1 cut(s) 3727
AspLEI GCGC 4 cut(s) 529, 2225, 2620, 3224
AsuC2I CCSGG 1 cut(s) 2900
AsuHPI GGTGA 9 cut(s) 37, 251, 1037, 1274, 1286, 1361, 2228, 2271, 4073
AsuII TTCGAA 2 cut(s) 786, 2435
AvaI CYCGRG 1 cut(s) 1088
AxyI CCTNAGG 1 cut(s) 1608
BaeGI GKGCMC 1 cut(s) 1627
BalI TGGCCA 1 cut(s) 1871
BamHI GGATCC 2 cut(s) 721, 3347
BanII GRGCYC 1 cut(s) 113
BauI CACGAG 2 cut(s) 571, 2583
Bbv12I GWGCWC 4 cut(s) 113, 1627, 2172, 4521
BbvCI CCTCAGC 1 cut(s) 2894
BceAI ACGGC 3 cut(s) 817, 2117, 3137
BciT130I CCWGG 7 cut(s) 55, 202, 1389, 1724, 3792, 3837, 4405
BciVI GTATCC 1 cut(s) 1742
BclI TGATCA 4 cut(s) 382, 1633, 3268, 3877
BcnI CCSGG 1 cut(s) 2900
BcuI ACTAGT 2 cut(s) 1466, 2375
BfmI CTRYAG 6 cut(s) 1118, 2079, 2847, 3678, 3783, 4106
BfuI GTATCC 1 cut(s) 1742
BglII AGATCT 3 cut(s) 711, 3577, 4501
BlpI GCTNAGC 1 cut(s) 1431
BmcAI AGTACT 1 cut(s) 374
Bme1390I CCNGG 8 cut(s) 55, 202, 1389, 1724, 2900, 3792, 3837, 4405
BmeT110I CYCGRG 1 cut(s) 1088
BmgBI CACGTC 1 cut(s) 3518
BmrFI CCNGG 8 cut(s) 55, 202, 1389, 1724, 2900, 3792, 3837, 4405
BmrI ACTGGG 3 cut(s) 956, 1758, 1985
BmuI ACTGGG 3 cut(s) 956, 1758, 1985
BoxI GACNNNNGTC 1 cut(s) 3384
BplI GAGNNNNNCTC 2 cut(s) 698, 730
BpmI CTGGAG 8 cut(s) 37, 273, 539, 647, 1185, 1410, 2394, 4548
Bpu10I CCTNAGC 1 cut(s) 2894
Bpu1102I GCTNAGC 1 cut(s) 1431
Bpu14I TTCGAA 2 cut(s) 786, 2435
BpuEI CTTGAG 3 cut(s) 671, 3829, 3923
BpuMI CCSGG 1 cut(s) 2900
Bsa29I ATCGAT 1 cut(s) 3864
BsaAI YACGTR 1 cut(s) 1571
BsaBI GATNNNNATC 2 cut(s) 93, 2145
BsaI GGTCTC 3 cut(s) 2658, 2990, 3615
BsaWI WCCGGW 2 cut(s) 856, 4340
BsaXI ACNNNNNCTCC 4 cut(s) 838, 868, 3162, 3192
Bsc4I CCNNNNNNNGG 7 cut(s) 54, 833, 1157, 1417, 1878, 2899, 3665
Bse118I RCCGGY 1 cut(s) 4340
Bse1I ACTGG 9 cut(s) 556, 951, 1753, 1980, 2406, 3490, 4349, 4364, 4561
Bse21I CCTNAGG 1 cut(s) 1608
Bse3DI GCAATG 4 cut(s) 509, 3290, 4035, 4427
Bse8I GATNNNNATC 2 cut(s) 93, 2145
BseBI CCWGG 7 cut(s) 55, 202, 1389, 1724, 3792, 3837, 4405
BseCI ATCGAT 1 cut(s) 3864
BseJI GATNNNNATC 2 cut(s) 93, 2145
BseLI CCNNNNNNNGG 7 cut(s) 54, 833, 1157, 1417, 1878, 2899, 3665
BseMI GCAATG 4 cut(s) 509, 3290, 4035, 4427
BseMII CTCAG 9 cut(s) 731, 1422, 1599, 2908, 3030, 4086, 4302, 4317, 4493
BseNI ACTGG 9 cut(s) 556, 951, 1753, 1980, 2406, 3490, 4349, 4364, 4561
BseRI GAGGAG 1 cut(s) 2789
BseSI GKGCMC 1 cut(s) 1627
BseYI CCCAGC 1 cut(s) 1899
BsgI GTGCAG 2 cut(s) 1491, 4134
Bsh1236I CGCG 2 cut(s) 237, 645
BshTI ACCGGT 1 cut(s) 4340
BshVI ATCGAT 1 cut(s) 3864
BsiHKAI GWGCWC 4 cut(s) 113, 1627, 2172, 4521
BsiHKCI CYCGRG 1 cut(s) 1088
BsiSI CCGG 4 cut(s) 194, 857, 2899, 4341
BslFI GGGAC 3 cut(s) 2412, 3496, 3642
BslI CCNNNNNNNGG 7 cut(s) 54, 833, 1157, 1417, 1878, 2899, 3665
BsmFI GGGAC 3 cut(s) 2412, 3496, 3642
BsmI GAATGC 2 cut(s) 1919, 2022
Bso31I GGTCTC 3 cut(s) 2658, 2990, 3615
BsoBI CYCGRG 1 cut(s) 1088
Bsp119I TTCGAA 2 cut(s) 786, 2435
Bsp1286I GDGCHC 4 cut(s) 113, 1627, 2172, 4521
Bsp1407I TGTACA 1 cut(s) 3999
Bsp1720I GCTNAGC 1 cut(s) 1431
Bsp19I CCATGG 3 cut(s) 3000, 3382, 3816
BspCNI CTCAG 9 cut(s) 730, 1423, 1600, 2907, 3031, 4087, 4303, 4318, 4492
BspDI ATCGAT 1 cut(s) 3864
BspFNI CGCG 2 cut(s) 237, 645
BspMAI CTGCAG 5 cut(s) 1122, 2083, 3682, 3787, 4110
BspT104I TTCGAA 2 cut(s) 786, 2435
BspTNI GGTCTC 3 cut(s) 2658, 2990, 3615
BsrDI GCAATG 4 cut(s) 509, 3290, 4035, 4427
BsrFI RCCGGY 1 cut(s) 4340
BsrGI TGTACA 1 cut(s) 3999
BsrI ACTGG 9 cut(s) 556, 951, 1753, 1980, 2406, 3490, 4349, 4364, 4561
BssAI RCCGGY 1 cut(s) 4340
BssSI CACGAG 2 cut(s) 571, 2583
BssT1I CCWWGG 7 cut(s) 1155, 1554, 3000, 3351, 3382, 3816, 4239
Bst2BI CACGAG 2 cut(s) 571, 2583
Bst2UI CCWGG 7 cut(s) 55, 202, 1389, 1724, 3792, 3837, 4405
Bst4CI ACNGT 9 cut(s) 524, 691, 761, 1137, 2211, 2510, 2673, 3194, 3244
Bst6I CTCTTC 2 cut(s) 1678, 4429
BstAPI GCANNNNNTGC 2 cut(s) 1478, 3686
BstAUI TGTACA 1 cut(s) 3999
BstBAI YACGTR 1 cut(s) 1571
BstBI TTCGAA 2 cut(s) 786, 2435
BstDSI CCRYGG 3 cut(s) 3000, 3382, 3816
BstFNI CGCG 2 cut(s) 237, 645
BstHHI GCGC 4 cut(s) 529, 2225, 2620, 3224
BstNI CCWGG 7 cut(s) 55, 202, 1389, 1724, 3792, 3837, 4405
BstNSI RCATGY 4 cut(s) 1310, 1991, 2184, 4544
BstPAI GACNNNNGTC 1 cut(s) 3384
BstSCI CCNGG 8 cut(s) 53, 200, 1387, 1722, 2898, 3790, 3835, 4403
BstSFI CTRYAG 6 cut(s) 1118, 2079, 2847, 3678, 3783, 4106
BstSLI GKGCMC 1 cut(s) 1627
BstSNI TACGTA 1 cut(s) 1571
BstUI CGCG 2 cut(s) 237, 645
BstX2I RGATCY 9 cut(s) 711, 721, 1600, 2252, 2578, 3347, 3577, 3898, 4501
BstXI CCANNNNNNTGG 1 cut(s) 4246
BstYI RGATCY 9 cut(s) 711, 721, 1600, 2252, 2578, 3347, 3577, 3898, 4501
Bsu15I ATCGAT 1 cut(s) 3864
Bsu36I CCTNAGG 1 cut(s) 1608
BsuI GTATCC 1 cut(s) 1742
BsuTUI ATCGAT 1 cut(s) 3864
BtgI CCRYGG 3 cut(s) 3000, 3382, 3816
BtgZI GCGATG 1 cut(s) 4056
BtrI CACGTC 1 cut(s) 3518
BtsI GCAGTG 3 cut(s) 1233, 3792, 4146
CaiI CAGNNNCTG 5 cut(s) 946, 1079, 1478, 1844, 3791
CfoI GCGC 4 cut(s) 529, 2225, 2620, 3224
Cfr10I RCCGGY 1 cut(s) 4340
ClaI ATCGAT 1 cut(s) 3864
CseI GACGC 3 cut(s) 243, 2114, 2732
Csp6I GTAC 9 cut(s) 40, 373, 1679, 2669, 3531, 3886, 4000, 4308, 4377
CspAI ACCGGT 1 cut(s) 4340
CviQI GTAC 9 cut(s) 40, 373, 1679, 2669, 3531, 3886, 4000, 4308, 4377
DraI TTTAAA 2 cut(s) 1108, 3765
DrdI GACNNNNNNGTC 1 cut(s) 746
DseDI GACNNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 2 cut(s) 1869, 1914
Eam1104I CTCTTC 2 cut(s) 1678, 4429
EarI CTCTTC 2 cut(s) 1678, 4429
EciI GGCGGA 1 cut(s) 3578
Ecl136II GAGCTC 1 cut(s) 111
Eco105I TACGTA 1 cut(s) 1571
Eco130I CCWWGG 7 cut(s) 1155, 1554, 3000, 3351, 3382, 3816, 4239
Eco147I AGGCCT 2 cut(s) 1895, 3174
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 3 cut(s) 2658, 2990, 3615
Eco32I GATATC 2 cut(s) 3630, 3862
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 6 cut(s) 446, 1244, 1782, 2138, 3534, 3543
Eco81I CCTNAGG 1 cut(s) 1608
Eco88I CYCGRG 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 4 cut(s) 167, 449, 1904, 3668
EcoRI GAATTC 1 cut(s) 1456
EcoRII CCWGG 7 cut(s) 53, 200, 1387, 1722, 3790, 3835, 4403
EcoRV GATATC 2 cut(s) 3630, 3862
EcoT14I CCWWGG 7 cut(s) 1155, 1554, 3000, 3351, 3382, 3816, 4239
EcoT22I ATGCAT 2 cut(s) 1308, 2038
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 7 cut(s) 1155, 1554, 3000, 3351, 3382, 3816, 4239
FalI AAGNNNNNCTT 4 cut(s) 3159, 3191, 4248, 4280
FaqI GGGAC 3 cut(s) 2412, 3496, 3642
FauI CCCGC 2 cut(s) 1231, 1365
FauNDI CATATG 2 cut(s) 2365, 3931
FbaI TGATCA 4 cut(s) 382, 1633, 3268, 3877
FriOI GRGCYC 1 cut(s) 113
GlaI GCGC 4 cut(s) 528, 2224, 2619, 3223
GsaI CCCAGC 1 cut(s) 1903
GsuI CTGGAG 8 cut(s) 37, 273, 539, 647, 1185, 1410, 2394, 4548
HapII CCGG 4 cut(s) 194, 857, 2899, 4341
HgaI GACGC 3 cut(s) 243, 2114, 2732
HhaI GCGC 4 cut(s) 529, 2225, 2620, 3224
Hin6I GCGC 4 cut(s) 527, 2223, 2618, 3222
HinP1I GCGC 4 cut(s) 527, 2223, 2618, 3222
HincII GTYRAC 4 cut(s) 751, 799, 904, 2104
HindII GTYRAC 4 cut(s) 751, 799, 904, 2104
HindIII AAGCTT 1 cut(s) 2500
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 4 cut(s) 194, 857, 2899, 4341
HphI GGTGA 9 cut(s) 37, 251, 1037, 1274, 1286, 1361, 2228, 2271, 4073
Hpy166II GTNNAC 9 cut(s) 751, 799, 827, 904, 1625, 2061, 2104, 3181, 4308
Hpy8I GTNNAC 9 cut(s) 751, 799, 827, 904, 1625, 2061, 2104, 3181, 4308
Hpy99I CGWCG 1 cut(s) 1101
HpyCH4III ACNGT 9 cut(s) 524, 691, 761, 1137, 2211, 2510, 2673, 3194, 3244
HpyCH4IV ACGT 6 cut(s) 42, 1096, 1570, 2910, 3517, 4473
HpySE526I ACGT 6 cut(s) 42, 1096, 1570, 2910, 3517, 4473
HspAI GCGC 4 cut(s) 527, 2223, 2618, 3222
Ksp22I TGATCA 4 cut(s) 382, 1633, 3268, 3877
KspAI GTTAAC 1 cut(s) 904
LmnI GCTCC 8 cut(s) 108, 116, 196, 418, 558, 923, 2167, 2776
MaeII ACGT 6 cut(s) 42, 1096, 1570, 2910, 3517, 4473
MfeI CAATTG 2 cut(s) 1010, 2949
MflI RGATCY 9 cut(s) 711, 721, 1600, 2252, 2578, 3347, 3577, 3898, 4501
MhlI GDGCHC 4 cut(s) 113, 1627, 2172, 4521
MlsI TGGCCA 1 cut(s) 1871
MluI ACGCGT 1 cut(s) 643
MluNI TGGCCA 1 cut(s) 1871
MlyI GAGTC 8 cut(s) 251, 266, 947, 1664, 1850, 1924, 3639, 3961
MmeI TCCRAC 3 cut(s) 396, 2008, 2597
Mox20I TGGCCA 1 cut(s) 1871
Mph1103I ATGCAT 2 cut(s) 1308, 2038
MroXI GAANNNNTTC 1 cut(s) 3727
MscI TGGCCA 1 cut(s) 1871
MslI CAYNNNNRTG 2 cut(s) 1311, 2048
Msp20I TGGCCA 1 cut(s) 1871
MspA1I CMGCKG 4 cut(s) 230, 2336, 3554, 4028
MspI CCGG 4 cut(s) 194, 857, 2899, 4341
MspR9I CCNGG 8 cut(s) 55, 202, 1389, 1724, 2900, 3792, 3837, 4405
MunI CAATTG 2 cut(s) 1010, 2949
Mva1269I GAATGC 2 cut(s) 1919, 2022
MvaI CCWGG 7 cut(s) 55, 202, 1389, 1724, 3792, 3837, 4405
MvnI CGCG 2 cut(s) 237, 645
NciI CCSGG 1 cut(s) 2900
NcoI CCATGG 3 cut(s) 3000, 3382, 3816
NdeI CATATG 2 cut(s) 2365, 3931
NmuCI GTSAC 7 cut(s) 43, 363, 980, 1262, 1858, 1927, 2041
NsiI ATGCAT 2 cut(s) 1308, 2038
NspI RCATGY 4 cut(s) 1310, 1991, 2184, 4544
NspV TTCGAA 2 cut(s) 786, 2435
PaeI GCATGC 1 cut(s) 1310
PaeR7I CTCGAG 1 cut(s) 1088
PceI AGGCCT 2 cut(s) 1895, 3174
PciI ACATGT 1 cut(s) 4540
PcsI WCGNNNNNNNCGW 3 cut(s) 642, 1576, 3503
PctI GAATGC 2 cut(s) 1919, 2022
PdmI GAANNNNTTC 1 cut(s) 3727
PflMI CCANNNNNTGG 1 cut(s) 833
PinAI ACCGGT 1 cut(s) 4340
PleI GAGTC 8 cut(s) 250, 265, 947, 1664, 1849, 1924, 3639, 3960
PpsI GAGTC 8 cut(s) 250, 265, 947, 1664, 1849, 1924, 3639, 3960
Ppu21I YACGTR 1 cut(s) 1571
PpuMI RGGWCCY 4 cut(s) 167, 449, 1904, 3668
PscI ACATGT 1 cut(s) 4540
PshAI GACNNNNGTC 1 cut(s) 3384
PshBI ATTAAT 3 cut(s) 2421, 2864, 3695
PsiI TTATAA 2 cut(s) 1796, 2625
Psp124BI GAGCTC 1 cut(s) 113
Psp5II RGGWCCY 4 cut(s) 167, 449, 1904, 3668
Psp6I CCWGG 7 cut(s) 53, 200, 1387, 1722, 3790, 3835, 4403
PspFI CCCAGC 1 cut(s) 1899
PspGI CCWGG 7 cut(s) 53, 200, 1387, 1722, 3790, 3835, 4403
PspPPI RGGWCCY 4 cut(s) 167, 449, 1904, 3668
PstI CTGCAG 5 cut(s) 1122, 2083, 3682, 3787, 4110
PstNI CAGNNNCTG 5 cut(s) 946, 1079, 1478, 1844, 3791
PsuI RGATCY 9 cut(s) 711, 721, 1600, 2252, 2578, 3347, 3577, 3898, 4501
PvuII CAGCTG 3 cut(s) 2336, 3554, 4028
RsaI GTAC 9 cut(s) 41, 374, 1680, 2670, 3532, 3887, 4001, 4309, 4378
RsaNI GTAC 9 cut(s) 40, 373, 1679, 2669, 3531, 3886, 4000, 4308, 4377
RseI CAYNNNNRTG 2 cut(s) 1311, 2048
SacI GAGCTC 1 cut(s) 113
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 8 cut(s) 251, 266, 947, 1664, 1850, 1924, 3639, 3961
ScrFI CCNGG 8 cut(s) 55, 202, 1389, 1724, 2900, 3792, 3837, 4405
SduI GDGCHC 4 cut(s) 113, 1627, 2172, 4521
SfcI CTRYAG 6 cut(s) 1118, 2079, 2847, 3678, 3783, 4106
Sfr274I CTCGAG 1 cut(s) 1088
SfuI TTCGAA 2 cut(s) 786, 2435
SlaI CTCGAG 1 cut(s) 1088
SmiMI CAYNNNNRTG 2 cut(s) 1311, 2048
SmlI CTYRAG 4 cut(s) 650, 1088, 3808, 3902
SmoI CTYRAG 4 cut(s) 650, 1088, 3808, 3902
SnaBI TACGTA 1 cut(s) 1571
SpeI ACTAGT 2 cut(s) 1466, 2375
SphI GCATGC 1 cut(s) 1310
SseBI AGGCCT 2 cut(s) 1895, 3174
SspI AATATT 2 cut(s) 408, 2724
SstI GAGCTC 1 cut(s) 113
StuI AGGCCT 2 cut(s) 1895, 3174
StyD4I CCNGG 8 cut(s) 53, 200, 1387, 1722, 2898, 3790, 3835, 4403
StyI CCWWGG 7 cut(s) 1155, 1554, 3000, 3351, 3382, 3816, 4239
TaaI ACNGT 9 cut(s) 524, 691, 761, 1137, 2211, 2510, 2673, 3194, 3244
TaiI ACGT 6 cut(s) 45, 1099, 1573, 2913, 3520, 4476
TaqI TCGA 8 cut(s) 350, 786, 1089, 1099, 2435, 2656, 3506, 3864
TaqII GACCGA 3 cut(s) 52, 951, 1425
TatI WGTACW 5 cut(s) 372, 2668, 3530, 3999, 4307
TauI GCSGC 6 cut(s) 191, 227, 230, 1919, 2092, 3563
TseFI GTSAC 7 cut(s) 43, 363, 980, 1262, 1858, 1927, 2041
Tsp45I GTSAC 7 cut(s) 43, 363, 980, 1262, 1858, 1927, 2041
TspGWI ACGGA 1 cut(s) 1532
Van91I CCANNNNNTGG 1 cut(s) 833
VneI GTGCAC 1 cut(s) 1623
VspI ATTAAT 3 cut(s) 2421, 2864, 3695
XceI RCATGY 4 cut(s) 1310, 1991, 2184, 4544
XcmI CCANNNNNNNNNTGG 1 cut(s) 4356
XhoI CTCGAG 1 cut(s) 1088
XmnI GAANNNNTTC 1 cut(s) 3727
ZrmI AGTACT 1 cut(s) 374
Zsp2I ATGCAT 2 cut(s) 1308, 2038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.