MD05G1143400.v1.1

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002933
Physical Location & Seq
Forward (+)
27438504 .. 27448248
9745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1143400.v1.1.491

Sequence Viewer

Length: 5148 bp
ATGTCCGCCTCCCAAACCCTAGGCGGCCCTTCCCGCGCCGGCCGCCTCCTCGGCCCCTCCCTCGACAAAATCATCAAGAACGCCGCCTGGCGCAAGCACTCCCATTTGGTCTCCGCCTGCAAATCCGCCCTCGACAAGCTCGATTCCGTCACCGACTCCTCCATCATCGATCCAAAGTCACCCATTTCTGGAATCTCTCTTTCCGACGCCGAGTTCGCCCTCGGCCCCGTCCTCCTCGCCTTAGATTCAGCTTACCCCAAGGTCGTCGACCCTGCCATCGACTGCGTCTACAAGCTCTTCTCCCTCGGCATCATCCACGGCGAGATTGACACTTCGGATCCCAAGCTTTTGCTTTTCAAGCTCGTCGAGTCGGTGTGTAAGTGCGCGGCGATCAGCGAGGAGCCGATCGAGCTCGGCGTGCTTCGGGTCTTGCTTGCCGCCGTGAGATCCCCGCGGGTTTTGATCCGGGGCGATTGTTTGGTGAATATTGTGAGGACCTGTTACAATGTGTACCTTGGTGGAGTGAATGGGACCAATCAAATCTGCGCCAAGTCCGTTTTGGCTCAGATAATGGTGATTGTTTTCACCAGAGTGGAGGAGGATTCGGTGAATGTCAGCATTTCTAGGGTTTCAGTGAACGAATTGTTGGAATTCACTGATAAGAATTTGAACGAAGGGAGCTCTATTTTTTTCTGCCAGAATTTTGTCAATGAGATTATGGATGCGAGCTATGGCGGTCCAGATGGTATAAAAACGGTTGCCTTCCCGGTTCCCAAATTACAAAACGGCAATGCCGATGGGGAGTCGAATAGTGATGGAGTTGAATCCGGTGACGGTTTTAGTAAGATTAGGGATGATGGGTATCTTTTGTTTAAGAATCTGTGTAAGTTGTCCATGAAATACTCATCACAGGAGCACTCTGATGATCAAATTCTCTTGAGGGGTAAAGTATTGTCCTTGGAGCTTTTGAAGGTGGTCATGGACAATGGGGGTCCAATCTGGCGCAACAATGAGAGGTTTCTCAATGCGATCAAGCAGTTCCTCTGCTTATCACTGTTAAAAAACAGTGCATTGTCGGTCATGGCCATTTTCCAGCTTCAGTGCTCTATTTTTACCAGCTTGTTGTCGAAATTCAGATCAGGGTTGAAAGCAGAAATTGGTATATTTTTTCCCATGCTTGTCCTACGCGTGCTTGAGAATGTCCTCCAGCCTAGTTTCTTGCAAAAAATGACGGTCCTCAATTTATTGGAGAAGATCTCTCAGGATTCGCAGATAATCGTTGACATTTTTGTCAACTTTGACTGTGATGTGGATGCCCCAAACATCTTTGAAAGGATTGTCAATGGCCTTCTGAAAACTGCTCTAGGACCTCCCACCGGTTCAACAACAACATTGTCTCCAGTCCAAGACATCACGTTCCGGCATGAGTCAGTAAAGTGCTTGGTTAGCATCATCAATTCAATGGGATCTTGGATGGACCGACAGCTGAGTCTGGGAGACTCCTATCTACCAAAGACCAATGAGAGCGACACTTCAGCTGAGAAAACAGAAAATTTGACCCCAAATGGTGAAGAAGGAGCTGCTTTTGATAACGAAGTACATCCAGAAGGAAATCCTGAAGTTTCAGATGCTGCAACTCTTGAGCAACGTCGAGCTTATAAGCTTGAACTCCAGAAAGGTGTCGCTCTGTTTAATAGGAAGCCTAACAAGGGCATTGAGTTTCTGATAAGCACCAAAAAAGTTGGTAGTTCCCCAGAAGATGTGGCTTCTTTCCTGAGGAACAACACTGCTGGCCTAAATGAAACCATGATTGGTGATTATTTAGGTGAAAGGGAAGAATTTCCGCTAAAAGTTATGCATGCTTATGTGGATTCCTTTAATTTCAAAGGGATGGATTTTGGTGAAGCAATAAGGTTTTTCCTGAGGGGCTTCAGGTTACCTGGAGAAGCACAGAAAATTGACCGCATCATGGAGAAGTTTGCTGAGCGTTATTGTAAATGCAGTCCAAATTCTTTTACAAGTGCGGATACTGCATATGTGCTTGCATACTCTGTGATACTACTCAATACAGATGCCCATAATAACACGGTGAAAGATAAGATGACCAAGGCTGACTTTATCCGGAACAACCGAGGAATAGATGATGGAAAGGATCTACCTGAGGAGTATCTTGGTGTCCTATATGATCAAATTGTCAAAAATGAAATTAAGATGAGCGCTGATTCTTCTGTACCACAGAGCAAGGAGGAAAACAGCTTTAATAAATTATTGGGCTTGGATGGTATCCTGAATCTGGTAACTGGGAAGCAGACTGAAGAAAAAGCATTGGGTGCAAATGGGCTTCTTATAAAGCGCATCCAAGAGCAATTTAAAGCAAAGTCAGGAAAATCAGAGTCTGTCTATCATGCTGTGACAGATGTGGCAATATTGAGGTTTATGGTGGAGGTCTGCTGGGGTCCTATGTTGGCTGCATTTAGTGTGACTCTTGACCAAAGTGATGACAGGCTTGCTACTTCTCAATGCTTACAAGGTTTCCGGCATGCTGTTCATGTTACTGCTTTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACTTCAGTAGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAAAATGTTGACGCTGTGAAGGCAATAATATCAATTGCCATTGAAGATGGTAATTATCTTCAGGAAGCATGGGAACACATATTAACGTGTCTTTCTCGAATAGAGCATCTGCAACTGTTGGGAGAAGGTGCACCTACTGATGCATCATTTTTGACTGGATCAAATGTTGAATCAGAGGAGAAAACACCAAAATCTACTGGCCTTTCTTCTCTGAAGAAAAAAGGAACTATACAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGACAGTACTAGTGTCGGGGTGAACACTTCTGGACTGGTAACTCCAGAACAGATTAATAACTTCATTTCAAACTTGAATTTGCTGGATCAGATTGGGAATTTTGAGTTGAATCATGTATTTGCTCATAGCCAAAGATTAAACAGTGAAGCTATAGTGGCTTTTGTGAAGGCCTTGAGCAAAGTTTCCATGGCAGAGTTGCAATCTCCAACAGACCCTCGCGTATTCAGCCTCACAAAAATAGTTGAAATAGCGCACTACAATATGAACCGCATCAGATTAGTGTGGTCTCGCATATGGAATGTTCTCTCAGACTTCTTTGTATCAGTTGGCTTGTCGGAGAACCTCTCAGTTGCAATATTTGTGATGGATTCATTACGTCAGCTTGCTATGAAATTCTTAGAGCGCGAGGAGCTGGCAAATTACAACTTCCAAAATGAATTTTTGAGACCATTTGTGATTGTTATGCAAAAAAGCAACTCTACAGAAATTAAGGAATTAATTGTTCGTTGCATTTCACAAATGGTTCTCAGCCGTGTCAATCATGTGAAATCTGGCTGGAAAAGTGTTTTTCTGGTTTTTACGGCAGCGGCAGCTGATGAGAGGAAAAATATTGTCTTGTTGGCCTTTGAGACCGTGGAAAAAATCGTCAGAGAATATTTCCCTTATATAACAGAGACAGAGACATTGACTTTTACTGATTGTGTTAGATGCCTCTTAACCTTCACGAATAGCAGATTCAATAGTGATGTTAGCCTCAATGCTATTGCATTTCTCCGGTTTTGTGCTGTCAAACTTGCTGAAGGAGGGCTTGTTTACAACAAGAGGAGTGAGCTTGATGTTTCATCCCTTCCAACAGCAAATGAGGATGCTTCAAATGGAGTAACTTTTAATGAGAAAGATGAACATGCATCCTTTTGGGTTCCATTGCTAACAGGTTTGTCAAAGCTAACATCAGATCCTAGATCAGCAATCAGGAAGGGTTCTTTGGAAGTTCTTTTTAACATTCTAAAGGATCATGGTCATCTCTTCTCACACTCCTTTTGGACTGCAATTTTCAATTCTGTTGTTTACCCCATATTTAGCTGTGTCAGCAATAAGAGAGACACATATATGAAAAATGACCAGTCTTTGCCAGTTTCAGTATCTCCGCGTCCTGAAGGAAGCACATGGGATTCTGAAACTTCTGCAGTTGCAGCAGACTGTCTTATGGACCTATTTCTCAGTTTTTTTGATACAGTAAGACCGCAACTACCAGGTGTGGTATCCATTCTGACAGGACTCATCCTAAGTCCTGTTCAGGGTACTGCTAGCACTGGGGTCGCAGGGTTAGTTCGTTTGGCTGGTGAAGTTGGTGAGAAGCTTTCAGAAGATGAATGGAGAGAGATCTTTCTGGCTTTAAAAGAAGCAGCCACATCTGCTGTGCCTGGATTTATGAAGGTGTTGAGAACCATGGATGACATCAATGTTCCTGGTCTTTCTAGATCTTATAGTGATATAGATTTGTCATCTGATCATGGGTTTACAAATGATGATCTTGAGGATGATAATCTGCAAACAGCATCATATTTGGTTTCAAGAACGAAGAGTCATATTGCTATGCAGCTACTTATCATACAGGTGGCTACTGATTTGTACAAACTCCACCTCGGATCGTTCTCAGTCGGAAACATTTCAATCCTCCTTGAAATATTTTCCCTCATTGCATCCCATGCTCACCAACTGAACTCGGAGACAATTCTGCACAAGAAGCTGCAAAAAGTGTGCTCTGTCCTGGAACTGACTGCCCCACCTTTGGTTCATTTTGAGAACGACTCTTACAAGAACTACTTAAGCTTCCTCCAAAACACCCTCGTGGACAGTCCTTCTCTGTCCGAGGAGATGAACATAGAAGCCCAACTCGTTGGAGCGTGTGAAAATATATTTCAGATCTACCTAAACTGTACCGAGCTTCGTTCTGCCGAGCAGAGGCCAGCTGATGAGCCAGTTTTGCACTGGATTCTTCCCTTGGGTACAGCAAAGAAAGAAGAATTGGCCACCAGGAGTGACTTAGCTGTGTCGGCGTTGCAGGTATTGAACAGTTTGGAAAAGGTTTCGTTTAGGAGGCACATTTCTCGGTTATTTCCGTTGTTGGTGGATCTTGTGAGGAGTGAGCACGCATCTGGAGAAGTTCAGCTTGTTCTAAGCAACATATTCCAATCATGTATAGGCCCAATAGTAATGCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1716

Amino Acids

189.7

Weight (kDa)

5.48

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 20 - 201 1.2e-23 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 284 - 442 3.4e-42 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 554 - 736 4e-74 Sec7 domain
Sec7-like_HDS PF09324 1104 - 1186 4.4e-30 Mon2/Sec7/BIG1-like, HDS
BIG2_C PF20252 1506 - 1705 9.9e-29 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1659, 2348
AasI GACNNNNNNGTC 2 cut(s) 1289, 1488
Acc36I ACCTGC 1 cut(s) 4975
AccI GTMKAC 2 cut(s) 267, 288
AccII CGCG 7 cut(s) 36, 386, 454, 1188, 3138, 3324, 4069
AccIII TCCGGA 1 cut(s) 2121
AcoI YGGCCR 3 cut(s) 40, 1083, 4950
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 8 cut(s) 512, 1599, 2232, 2926, 4222, 4553, 4861, 4930
AfeI AGCGCT 1 cut(s) 2218
AfiI CCNNNNNNNGG 8 cut(s) 188, 1376, 1709, 1969, 2293, 4217, 4711, 4884
AflII CTTAAG 1 cut(s) 4747
AflIII ACRYGT 2 cut(s) 1186, 2732
AgeI ACCGGT 1 cut(s) 1376
AhlI ACTAGT 1 cut(s) 2927
AjnI CCWGG 7 cut(s) 86, 1939, 4171, 4342, 4387, 4689, 4955
AjuI GAANNNNNNNTTGG 8 cut(s) 629, 661, 1794, 1826, 3887, 3919, 4931, 4963
AleI CACNNNNGTG 2 cut(s) 590, 4769
Alw21I GWGCWC 7 cut(s) 414, 683, 918, 1106, 2779, 4685, 5073
Alw44I GTGCAC 1 cut(s) 2775
AlwNI CAGNNNCTG 2 cut(s) 1631, 2396
Aor13HI TCCGGA 1 cut(s) 2121
Aor51HI AGCGCT 1 cut(s) 2218
ApaLI GTGCAC 1 cut(s) 2775
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AseI ATTAAT 2 cut(s) 2973, 3416
AsiGI ACCGGT 1 cut(s) 1376
Asp700I GAANNNNTTC 2 cut(s) 1839, 4588
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 2219, 2355, 3172, 3324
AsuC2I CCSGG 2 cut(s) 467, 767
AsuNHI GCTAGC 1 cut(s) 4226
AvaII GGWCC 9 cut(s) 495, 531, 737, 992, 1234, 1367, 1477, 2456, 4129
AvrII CCTAGG 1 cut(s) 19
AxyI CCTNAGG 3 cut(s) 1775, 1922, 2160
BaeGI GKGCMC 1 cut(s) 2779
BalI TGGCCA 2 cut(s) 1085, 4952
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 2 cut(s) 414, 683
BauI CACGAG 1 cut(s) 4769
Bbv12I GWGCWC 7 cut(s) 414, 683, 918, 1106, 2779, 4685, 5073
BceAI ACGGC 5 cut(s) 334, 425, 802, 3435, 3516
BcgI CGANNNNNNTGC 4 cut(s) 4219, 4253, 5012, 5046
BciT130I CCWGG 7 cut(s) 88, 1941, 4173, 4344, 4389, 4691, 4957
BciVI GTATCC 3 cut(s) 2020, 2294, 4192
BclI TGATCA 3 cut(s) 925, 2185, 4429
BcnI CCSGG 2 cut(s) 467, 767
BcuI ACTAGT 1 cut(s) 2927
BfaI CTAG 8 cut(s) 20, 624, 1212, 1364, 2928, 3879, 4227, 4398
BfmI CTRYAG 4 cut(s) 2631, 3069, 3399, 4104
BfoI RGCGCY 1 cut(s) 2220
BfrI CTTAAG 1 cut(s) 4747
BfuAI ACCTGC 1 cut(s) 4975
BfuI GTATCC 3 cut(s) 2020, 2294, 4192
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 4 cut(s) 1254, 4302, 4400, 4845
BlnI CCTAGG 1 cut(s) 19
BlpI GCTNAGC 1 cut(s) 1983
BmcAI AGTACT 1 cut(s) 2926
Bme1390I CCNGG 9 cut(s) 88, 467, 767, 1941, 4173, 4344, 4389, 4691, 4957
Bme18I GGWCC 9 cut(s) 495, 531, 737, 992, 1234, 1367, 1477, 2456, 4129
BmiI GGNNCC 9 cut(s) 55, 226, 339, 402, 532, 771, 993, 2457, 3840
BmrFI CCNGG 9 cut(s) 88, 467, 767, 1941, 4173, 4344, 4389, 4691, 4957
BmrI ACTGGG 2 cut(s) 2310, 4242
BmtI GCTAGC 1 cut(s) 4230
BmuI ACTGGG 2 cut(s) 2310, 4242
BplI GAGNNNNNCTC 6 cut(s) 1241, 1273, 3248, 3280, 4715, 4747
BpmI CTGGAG 6 cut(s) 1190, 1383, 1655, 1962, 2946, 5100
Bpu1102I GCTNAGC 1 cut(s) 1983
BpuEI CTTGAG 5 cut(s) 958, 1214, 1661, 3112, 4475
BpuMI CCSGG 2 cut(s) 467, 767
Bsa29I ATCGAT 1 cut(s) 168
BsaBI GATNNNNATC 2 cut(s) 861, 4464
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 4 cut(s) 115, 3210, 3358, 3542
BsaWI WCCGGW 4 cut(s) 827, 1376, 2121, 3693
BsaXI ACNNNNNCTCC 4 cut(s) 1381, 1411, 3714, 3744
Bsc4I CCNNNNNNNGG 8 cut(s) 188, 1376, 1709, 1969, 2293, 4217, 4711, 4884
Bse118I RCCGGY 2 cut(s) 38, 1376
Bse21I CCTNAGG 3 cut(s) 1775, 1922, 2160
Bse3DI GCAATG 3 cut(s) 796, 3842, 4617
Bse8I GATNNNNATC 2 cut(s) 861, 4464
BseAI TCCGGA 1 cut(s) 2121
BseBI CCWGG 7 cut(s) 88, 1941, 4173, 4344, 4389, 4691, 4957
BseCI ATCGAT 1 cut(s) 168
BseJI GATNNNNATC 2 cut(s) 861, 4464
BseLI CCNNNNNNNGG 8 cut(s) 188, 1376, 1709, 1969, 2293, 4217, 4711, 4884
BseMI GCAATG 3 cut(s) 796, 3842, 4617
BseSI GKGCMC 1 cut(s) 2779
BseX3I CGGCCG 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 2451
BsgI GTGCAG 1 cut(s) 4643
Bsh1236I CGCG 7 cut(s) 36, 386, 454, 1188, 3138, 3324, 4069
Bsh1285I CGRYCG 2 cut(s) 43, 408
BshTI ACCGGT 1 cut(s) 1376
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 2 cut(s) 43, 408
BsiHKAI GWGCWC 7 cut(s) 414, 683, 918, 1106, 2779, 4685, 5073
BsiSI CCGG 9 cut(s) 39, 466, 767, 828, 1377, 1420, 2122, 2536, 3694
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 8 cut(s) 188, 1376, 1709, 1969, 2293, 4217, 4711, 4884
BsmFI GGGAC 1 cut(s) 544
BsmI GAATGC 1 cut(s) 2574
Bso31I GGTCTC 4 cut(s) 115, 3210, 3358, 3542
Bsp1286I GDGCHC 7 cut(s) 414, 683, 918, 1106, 2779, 4685, 5073
Bsp13I TCCGGA 1 cut(s) 2121
Bsp1407I TGTACA 1 cut(s) 4551
Bsp1720I GCTNAGC 1 cut(s) 1983
Bsp19I CCATGG 2 cut(s) 3105, 4368
BspDI ATCGAT 1 cut(s) 168
BspEI TCCGGA 1 cut(s) 2121
BspFNI CGCG 7 cut(s) 36, 386, 454, 1188, 3138, 3324, 4069
BspLI GGNNCC 9 cut(s) 55, 226, 339, 402, 532, 771, 993, 2457, 3840
BspMAI CTGCAG 2 cut(s) 2635, 4108
BspMI ACCTGC 1 cut(s) 4975
BspOI GCTAGC 1 cut(s) 4230
BspQI GCTCTTC 1 cut(s) 302
BspTI CTTAAG 1 cut(s) 4747
BspTNI GGTCTC 4 cut(s) 115, 3210, 3358, 3542
BsrDI GCAATG 3 cut(s) 796, 3842, 4617
BsrFI RCCGGY 2 cut(s) 38, 1376
BsrGI TGTACA 1 cut(s) 4551
BssAI RCCGGY 2 cut(s) 38, 1376
BssNI GRCGYC 1 cut(s) 207
BssSI CACGAG 1 cut(s) 4769
BssT1I CCWWGG 8 cut(s) 19, 258, 514, 957, 2106, 3105, 4368, 4923
Bst2BI CACGAG 1 cut(s) 4769
Bst2UI CCWGG 7 cut(s) 88, 1941, 4173, 4344, 4389, 4691, 4957
Bst6I CTCTTC 3 cut(s) 302, 3950, 4496
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 4747
BstAPI GCANNNNNTGC 2 cut(s) 2330, 4628
BstAUI TGTACA 1 cut(s) 4551
BstDSI CCRYGG 5 cut(s) 316, 452, 3105, 3552, 4368
BstEII GGTNACC 1 cut(s) 1935
BstFNI CGCG 7 cut(s) 36, 386, 454, 1188, 3138, 3324, 4069
BstH2I RGCGCY 1 cut(s) 2220
BstHHI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 2219, 2355, 3172, 3324
BstMCI CGRYCG 2 cut(s) 43, 408
BstNI CCWGG 7 cut(s) 88, 1941, 4173, 4344, 4389, 4691, 4957
BstNSI RCATGY 3 cut(s) 1862, 2543, 3827
BstPI GGTNACC 1 cut(s) 1935
BstSCI CCNGG 9 cut(s) 86, 465, 765, 1939, 4171, 4342, 4387, 4689, 4955
BstSFI CTRYAG 4 cut(s) 2631, 3069, 3399, 4104
BstSLI GKGCMC 1 cut(s) 2779
BstUI CGCG 7 cut(s) 36, 386, 454, 1188, 3138, 3324, 4069
BstXI CCANNNNNNTGG 1 cut(s) 4820
BstZI CGGCCG 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 3 cut(s) 1775, 1922, 2160
BsuI GTATCC 3 cut(s) 2020, 2294, 4192
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 5 cut(s) 316, 452, 3105, 3552, 4368
BtsI GCAGTG 1 cut(s) 1785
BtsIMutI CAGTG 9 cut(s) 639, 654, 1052, 1072, 1106, 1785, 3067, 4230, 4909
BveI ACCTGC 1 cut(s) 4975
CaiI CAGNNNCTG 2 cut(s) 1631, 2396
CfoI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 2219, 2355, 3172, 3324
Cfr10I RCCGGY 2 cut(s) 38, 1376
Cfr42I CCGCGG 1 cut(s) 455
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 4 cut(s) 215, 274, 2666, 4058
CsiI ACCWGGT 1 cut(s) 4171
Csp6I GTAC 8 cut(s) 511, 1598, 2231, 2925, 4221, 4552, 4860, 4929
CspAI ACCGGT 1 cut(s) 1376
CviQI GTAC 8 cut(s) 511, 1598, 2231, 2925, 4221, 4552, 4860, 4929
DraI TTTAAA 2 cut(s) 2371, 4317
DrdI GACNNNNNNGTC 2 cut(s) 1289, 1488
DseDI GACNNNNNNGTC 2 cut(s) 1289, 1488
EaeI YGGCCR 3 cut(s) 40, 1083, 4950
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 3 cut(s) 302, 3950, 4496
EarI CTCTTC 3 cut(s) 302, 3950, 4496
EciI GGCGGA 2 cut(s) 103, 115
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco130I CCWWGG 8 cut(s) 19, 258, 514, 957, 2106, 3105, 4368, 4923
Eco147I AGGCCT 1 cut(s) 3089
Eco24I GRGCYC 2 cut(s) 414, 683
Eco31I GGTCTC 4 cut(s) 115, 3210, 3358, 3542
Eco47I GGWCC 9 cut(s) 495, 531, 737, 992, 1234, 1367, 1477, 2456, 4129
Eco47III AGCGCT 1 cut(s) 2218
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
Eco81I CCTNAGG 3 cut(s) 1775, 1922, 2160
Eco91I GGTNACC 1 cut(s) 1935
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 3 cut(s) 495, 1367, 2456
EcoO65I GGTNACC 1 cut(s) 1935
EcoRI GAATTC 1 cut(s) 650
EcoRII CCWGG 7 cut(s) 86, 1939, 4171, 4342, 4387, 4689, 4955
EcoT14I CCWWGG 8 cut(s) 19, 258, 514, 957, 2106, 3105, 4368, 4923
EcoT22I ATGCAT 4 cut(s) 1860, 2590, 2791, 3829
EcoT38I GRGCYC 2 cut(s) 414, 683
ErhI CCWWGG 8 cut(s) 19, 258, 514, 957, 2106, 3105, 4368, 4923
FalI AAGNNNNNCTT 8 cut(s) 2099, 2131, 3711, 3743, 4730, 4762, 5076, 5108
FaqI GGGAC 1 cut(s) 544
FauI CCCGC 3 cut(s) 41, 447, 459
FauNDI CATATG 3 cut(s) 2035, 2917, 3212
FbaI TGATCA 3 cut(s) 925, 2185, 4429
FblI GTMKAC 2 cut(s) 267, 288
FriOI GRGCYC 2 cut(s) 414, 683
FspBI CTAG 8 cut(s) 20, 624, 1212, 1364, 2928, 3879, 4227, 4398
GlaI GCGC 9 cut(s) 37, 92, 385, 547, 1004, 2218, 2354, 3171, 3323
GsaI CCCAGC 1 cut(s) 2455
GsuI CTGGAG 6 cut(s) 1190, 1383, 1655, 1962, 2946, 5100
HaeII RGCGCY 1 cut(s) 2220
HapII CCGG 9 cut(s) 39, 466, 767, 828, 1377, 1420, 2122, 2536, 3694
HgaI GACGC 4 cut(s) 215, 274, 2666, 4058
HhaI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 2219, 2355, 3172, 3324
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 9 cut(s) 36, 91, 384, 546, 1003, 2217, 2353, 3170, 3322
HinP1I GCGC 9 cut(s) 36, 91, 384, 546, 1003, 2217, 2353, 3170, 3322
HincII GTYRAC 4 cut(s) 268, 1282, 1294, 2656
HindII GTYRAC 4 cut(s) 268, 1282, 1294, 2656
HindIII AAGCTT 4 cut(s) 344, 1661, 4277, 4750
HpaII CCGG 9 cut(s) 39, 466, 767, 828, 1377, 1420, 2122, 2536, 3694
Hpy99I CGWCG 4 cut(s) 209, 269, 368, 1653
HpyCH4IV ACGT 4 cut(s) 1415, 1648, 2732, 3295
HpySE526I ACGT 4 cut(s) 1415, 1648, 2732, 3295
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 9 cut(s) 36, 91, 384, 546, 1003, 2217, 2353, 3170, 3322
Kpn2I TCCGGA 1 cut(s) 2121
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 3 cut(s) 925, 2185, 4429
KspI CCGCGG 1 cut(s) 455
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 7 cut(s) 400, 678, 913, 961, 1577, 3328, 4823
MabI ACCWGGT 1 cut(s) 4171
MaeI CTAG 8 cut(s) 20, 624, 1212, 1364, 2928, 3879, 4227, 4398
MaeII ACGT 4 cut(s) 1415, 1648, 2732, 3295
MfeI CAATTG 1 cut(s) 2679
MhlI GDGCHC 7 cut(s) 414, 683, 918, 1106, 2779, 4685, 5073
MlsI TGGCCA 2 cut(s) 1085, 4952
MluI ACGCGT 1 cut(s) 1186
MluNI TGGCCA 2 cut(s) 1085, 4952
MmeI TCCRAC 7 cut(s) 228, 627, 3149, 3234, 3794, 4561, 4801
Mox20I TGGCCA 2 cut(s) 1085, 4952
Mph1103I ATGCAT 4 cut(s) 1860, 2590, 2791, 3829
MroI TCCGGA 1 cut(s) 2121
MroNI GCCGGC 1 cut(s) 38
MroXI GAANNNNTTC 2 cut(s) 1839, 4588
MscI TGGCCA 2 cut(s) 1085, 4952
MslI CAYNNNNRTG 6 cut(s) 590, 921, 1863, 4028, 4535, 4769
Msp20I TGGCCA 2 cut(s) 1085, 4952
MspA1I CMGCKG 7 cut(s) 454, 1486, 1538, 2888, 3506, 3512, 4892
MspCI CTTAAG 1 cut(s) 4747
MspI CCGG 9 cut(s) 39, 466, 767, 828, 1377, 1420, 2122, 2536, 3694
MspR9I CCNGG 9 cut(s) 88, 467, 767, 1941, 4173, 4344, 4389, 4691, 4957
MunI CAATTG 1 cut(s) 2679
Mva1269I GAATGC 1 cut(s) 2574
MvaI CCWGG 7 cut(s) 88, 1941, 4173, 4344, 4389, 4691, 4957
MvnI CGCG 7 cut(s) 36, 386, 454, 1188, 3138, 3324, 4069
NaeI GCCGGC 1 cut(s) 40
NciI CCSGG 2 cut(s) 467, 767
NcoI CCATGG 2 cut(s) 3105, 4368
NdeI CATATG 3 cut(s) 2035, 2917, 3212
NgoMIV GCCGGC 1 cut(s) 38
NheI GCTAGC 1 cut(s) 4226
NlaIV GGNNCC 9 cut(s) 55, 226, 339, 402, 532, 771, 993, 2457, 3840
NmeAIII GCCGAG 6 cut(s) 30, 201, 235, 285, 393, 4903
NmuCI GTSAC 7 cut(s) 148, 177, 830, 2410, 2479, 2593, 4961
NsiI ATGCAT 4 cut(s) 1860, 2590, 2791, 3829
NspI RCATGY 3 cut(s) 1862, 2543, 3827
OliI CACNNNNGTG 2 cut(s) 590, 4769
PaeI GCATGC 2 cut(s) 1862, 2543
PceI AGGCCT 1 cut(s) 3089
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 414, 792
PctI GAATGC 1 cut(s) 2574
PdiI GCCGGC 1 cut(s) 40
PdmI GAANNNNTTC 2 cut(s) 1839, 4588
PflFI GACNNNGTC 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 4689
PinAI ACCGGT 1 cut(s) 1376
Ple19I CGATCG 1 cut(s) 408
PpuMI RGGWCCY 3 cut(s) 495, 1367, 2456
PshBI ATTAAT 2 cut(s) 2973, 3416
PsiI TTATAA 2 cut(s) 1659, 2348
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp5II RGGWCCY 3 cut(s) 495, 1367, 2456
Psp6I CCWGG 7 cut(s) 86, 1939, 4171, 4342, 4387, 4689, 4955
PspEI GGTNACC 1 cut(s) 1935
PspFI CCCAGC 1 cut(s) 2451
PspGI CCWGG 7 cut(s) 86, 1939, 4171, 4342, 4387, 4689, 4955
PspN4I GGNNCC 9 cut(s) 55, 226, 339, 402, 532, 771, 993, 2457, 3840
PspPPI RGGWCCY 3 cut(s) 495, 1367, 2456
PsrI GAACNNNNNNTAC 2 cut(s) 4718, 4750
PstI CTGCAG 2 cut(s) 2635, 4108
PstNI CAGNNNCTG 2 cut(s) 1631, 2396
PsyI GACNNNGTC 1 cut(s) 284
PvuI CGATCG 1 cut(s) 408
PvuII CAGCTG 5 cut(s) 1486, 1538, 2888, 3512, 4892
RsaI GTAC 8 cut(s) 512, 1599, 2232, 2926, 4222, 4553, 4861, 4930
RsaNI GTAC 8 cut(s) 511, 1598, 2231, 2925, 4221, 4552, 4860, 4929
RseI CAYNNNNRTG 6 cut(s) 590, 921, 1863, 4028, 4535, 4769
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 1 cut(s) 455
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 302
ScaI AGTACT 1 cut(s) 2926
ScrFI CCNGG 9 cut(s) 88, 467, 767, 1941, 4173, 4344, 4389, 4691, 4957
SduI GDGCHC 7 cut(s) 414, 683, 918, 1106, 2779, 4685, 5073
SexAI ACCWGGT 1 cut(s) 4171
SfcI CTRYAG 4 cut(s) 2631, 3069, 3399, 4104
Sfr303I CCGCGG 1 cut(s) 455
SgrBI CCGCGG 1 cut(s) 455
SinI GGWCC 9 cut(s) 495, 531, 737, 992, 1234, 1367, 1477, 2456, 4129
SmiMI CAYNNNNRTG 6 cut(s) 590, 921, 1863, 4028, 4535, 4769
SmlI CTYRAG 6 cut(s) 937, 1193, 1640, 3091, 4454, 4747
SmoI CTYRAG 6 cut(s) 937, 1193, 1640, 3091, 4454, 4747
SpeI ACTAGT 1 cut(s) 2927
SphI GCATGC 2 cut(s) 1862, 2543
SseBI AGGCCT 1 cut(s) 3089
SspI AATATT 6 cut(s) 487, 2427, 3276, 3529, 3575, 4608
SspMI CTAG 8 cut(s) 20, 624, 1212, 1364, 2928, 3879, 4227, 4398
SstI GAGCTC 2 cut(s) 414, 683
StuI AGGCCT 1 cut(s) 3089
StyD4I CCNGG 9 cut(s) 86, 465, 765, 1939, 4171, 4342, 4387, 4689, 4955
StyI CCWWGG 8 cut(s) 19, 258, 514, 957, 2106, 3105, 4368, 4923
TaiI ACGT 4 cut(s) 1418, 1651, 2735, 3298
TaqII GACCGA 2 cut(s) 1066, 1494
TatI WGTACW 3 cut(s) 1597, 2924, 4551
TauI GCSGC 6 cut(s) 27, 45, 86, 389, 440, 3509
TscAI CASTG 9 cut(s) 639, 661, 1059, 1072, 1106, 1792, 3067, 4237, 4916
TseFI GTSAC 7 cut(s) 148, 177, 830, 2410, 2479, 2593, 4961
Tsp45I GTSAC 7 cut(s) 148, 177, 830, 2410, 2479, 2593, 4961
TspGWI ACGGA 3 cut(s) 136, 544, 5031
TspRI CASTG 9 cut(s) 639, 661, 1059, 1072, 1106, 1792, 3067, 4237, 4916
Tth111I GACNNNGTC 1 cut(s) 284
Vha464I CTTAAG 1 cut(s) 4747
VneI GTGCAC 1 cut(s) 2775
VpaK11BI GGWCC 9 cut(s) 495, 531, 737, 992, 1234, 1367, 1477, 2456, 4129
VspI ATTAAT 2 cut(s) 2973, 3416
XbaI TCTAGA 1 cut(s) 4397
XceI RCATGY 3 cut(s) 1862, 2543, 3827
XcmI CCANNNNNNNNNTGG 2 cut(s) 556, 4908
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 267, 288
XmnI GAANNNNTTC 2 cut(s) 1839, 4588
XspI CTAG 8 cut(s) 20, 624, 1212, 1364, 2928, 3879, 4227, 4398
ZrmI AGTACT 1 cut(s) 2926
Zsp2I ATGCAT 4 cut(s) 1860, 2590, 2791, 3829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.