FvH4_2g17050

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002933
Physical Location & Seq
Forward (+)
14764073 .. 14776501
12429 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17050.t8

Sequence Viewer

Length: 3873 bp
ATGATTCACAGATTATCATTGACATTTTTGTCAACTATGACTGTGATGTGGATTCTCCAAACATTTTCGAAAGATGTTTGCAGGATTGTCAACGGCCTTCTGAAAACTGCTCTAGGTCCGCCTTCTGGTTCGACAACAACATTATCTCCAGTTCAGGATATTACTTTTCGGCATGAATCAGTGAAGTGCCTGGTTAACATCATTAAGTCAATGGGAGCTTGGATGGACCGACAGAGACTGGGAGACTCCTATCTTCCAAAGACCAATGAGAGTGACACTCCATCAGAGAAAACAGAGAATCAATTGACCCTCAATGGTGAAGAAGGAATTGTTTCAGAAAATGATGTACAACCAGAAGGAAATTCAGATGCTGTAACACTCGAGCAACGCCGAGCTTTTAAACTTGAACTGCAGAAAGGTATCTCACTGTTCAATAGGAAACCTTCCAAGGGCATTGAGTTTCTGATAAGTACCAAAAAAATTGGGGGTTCTCCAGCAGATGTGGCTTCTTTTCTGAGAAACAACACTACGGGCCTAAATGAAACCATGATCGGTGACTATTTGGGTGAAAGAGAAGAATTTCCTCTGAAAGTTATGCATGCTTATGTAGATTCCTTTAATTTTAAAGGGATGGATTTTGGTGAAGCAATTAGGTTTTTCCTGCGGGGATTCAAGTTGCCTGGAGAAGCACAGAAAATTGACCGAATCATGGAGAAGTTCGCTGAGCGCTATTGTAAATGCAGTCCAAATTCATTTACTAGTGCAGATACTGCTTATGTCCTTGCATACTCTGTGATAATGCTCAACACGGATGCCCATAATAGCATGGTTAAAGATAAGATGACCAAGGCTGATTTTATACGCAACAATCGAGGAATAGATGATGGCAAAGATCTACCTGAGGAATATCTTGGTGCTCTATATGATCAGATTGTAAAAAATGAAATTAAGATGAAGGCCGACTCTTCTGTACCACAGAGCAAGCAAGAAAACAGCTTTAACAAATTACTGGGCCTGGATGGTATCCTGAATCTAGTAACCGGGAAGCAGACTGAAGAAAAAGCGTTGGGTGCAAACGGGCTTCTTATAAAGGACATCCAAGAGCAATTTAAGGCGAAGTCAGGGAAATCAGAGTCTGTTTATCATTCTGTGACAGATGTGGCGATATTGAGGTTTATGGTGGAGGTCTGCTGGGGTCCTATGCTGGCCGCATTCAGTGTGACTCTTGACCAAAGTGATGACAGGCTTGCTACCTCTCAATGCTTACATGGTTTTCGATATGCCATTCATGTTACTGCTCTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACATCAATGGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAAAATGTGGACGCTGTGAAGGCAATAATAACAATTGCCATCGAAGATGGTAATCATCTTCAGGAAGCATGGGAGCACATATTAACATGCCTTTCACGAATTGAGCATCTGCAATTGTTGGGTGAGGGCGCACCTACTGATGCAACCTTTTTCAGTGGATCTAATAATGAATTGGATGACAAATCACCAAGGCCTATTGGTTTTGCTTCTCTAAAGAAAAAAGGAACTATCCAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGATAGCACTAGTATTGGAATCAACACTTCGGGACTGGTATCTCCAGAACAGATTAATAACTTCATTTCAAATTTGAATTTGCTGGATCAGATTGGAAACTTTGAGTTGAACCATGTATTTGCTCATAGCCAAAGCTTGAACAGTGAAGCCATAGTTGCTTTTGTGAAGTCCTTGTGCAAAGTTTCCATGTCAGAATTGCAGTCTCCAACAGACCCTCGTGTTTTCAGCCTCACAAAAATAGTCGAAATAGCGCATTACAATATGAATCGTATCAGATTAGTGTGGTCTCGAATATGGAATGTACTGTCAGATTTTTTTGTATCAGTTGGCTTATCAGAGAATCTTTCAGTTGCAATATTTGTGATGGATTCATTGCGTCAGCTTGCTATGAAATTCTTAGAGCGTGAGGAGCTGGCAAATTACAATTTCCAGAATGAATTTTTGAGACCATTTGTGATTGTTATGCAGAAAAGCAGCTCTACCGAGATTAGGGAATTAATAGTTCGGTGCATTTCACAGATGGTCCTCAGCCGGGTCAAAAATGTGAAATCTGGCTGGAAAAGTGTTTTTATGGTTTTCACAACTGCTGCCGCTGATGAGAGGAAAAATATAGTCTTATTGGCTTTTGAGACCATGGAAAAGATAGTCAGAGAATATTTCCCTTATATTACGGAGACAGAGGCATTGACTTTTACTGATTGCGTTAAATGCCTCCTGACCTTCACAAATAGCAGATTCAATAGTGATGTTAGCCTCAATGCTATTGCATTTCTGCGTTTCTGTGCTGTCAAACTTGCCGAAGGAGGCCTTGTTTACAATAAGAACAGTGAAGTTGATGGTTCTTCAATTCCTAACGCAAATGAAGGTGCTTCAACTGTTGAGGATTTCAATGATAAAGATGATCATACATCTTTTTGGGTGCCATTGCTAACAGGTTTGTCAAAACTAACAAATGATCCTAGATCAGCTATCAGGAAGGGATCCTTGGAAGTTCTTTTCAACATTTTAAAGGACCATGGTCATCTTTTCTCAAACCTGTTTTGGACTGCGGTTTTCAATTCTGTTATTTTCCCCATTTTTAAAGGTGCCAGCGATAAGAAAGACACTGACATGAAAAATGGCCAGTCCTCGCCAGTGTCAATGTCCCCACGTCCTGAGGGAAGTACATGGGATTCTGAAACTTCGGCTGTTGCGACGGATTGTCTCATAGATTTGTTTGTCAGTTTCTTTGATGTTGTTAGGCCTCAACTACCAAGTGTGATATCCATTCTGACAGGACTAATCAGAAGTCCTGTTCAGGGTCCTGCTACCGCAGGAGTTGGTGCATTAGTGCGTTTGTCCAGTGAGGTGGGTAGCAGGTTTTCAGAAGATGAATGGAATGCAATTTTTCTGGGTTTAAAGGAGGCTGCCACATCTGCTGTGCCTGGATTTTTGAAGGTCTTGAGAACCATGGACAATATCAATGTTCCTGGTCTTTCTGATTCTTATAGTGATATCGATATGTCGTCTGATCAGGGGTACTCAAATGAGGATCTTGAGGATGATAATTTGCAAACAGCTTCATACGTGGTGTTAAGAGTGAAGAGTCATGTTGCTATGCAGCTACTCATCTTACAGGTTGCTGCTGATTTGTACAAAATACACCTAGAAACCTTCTCAGCTGCTAACATGACAGTTCTTCTTGAAGTATTTTCTCTTGTGTCATCACATGCTCATGAACTGAACTCTGAGACAATTCTACAGAAGAAGCTGGAAAAAGTGTGTTCCATCCTAGAACTTACTGCACCACCTATTGTTCATTTTGAGAATGAATCTTACAAAAACTTTCTCAACTTCCTCCAAAACTCACTTGTGGATAATCCGTCTCTTTCCAAGAAAATGAACATAGAAGCTAAACTCGTGGCAGTGTGTGAAGACATATTGCAGATATACCTAAAATGTACTGAGCTTCAATCTTCTGAACAGAAGCCAGTTTTGCACTGGATTCTTCCTTTGGGTACAGCAAAGAAGGAGGAATTGGCTACCAGGACTTTCTTAGCCGTCTCAGCATTGCAGGCATTGAGTGGTTTGGAAAAGGCCTCATTTAGGAGGCACGTTTCTCAGTTATTTCCTTTGTTGGTAGATCTTGTGAAGAGTGAGCACACTTCTGGAGAAGTTCAACATGTTCTAAGCAACATATTCCAGTCATGTATAGGCCCAATAATAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1291

Amino Acids

143.52

Weight (kDa)

5.66

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 20 - 201 1.2e-21 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 289 - 447 6.1e-42 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 556 - 738 5.6e-75 Sec7 domain
Sec7-like_HDS PF09324 1106 - 1188 1.4e-30 Mon2/Sec7/BIG1-like, HDS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1088
AasI GACNNNNNNGTC 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 3006
AccB1I GGYRCC 2 cut(s) 2578, 2744
AciI CCGC 6 cut(s) 119, 664, 1209, 2250, 2708, 2970
AclWI GGATC 6 cut(s) 1552, 1752, 2609, 2634, 2647, 3198
AcoI YGGCCR 2 cut(s) 1206, 2779
AcsI RAATTY 7 cut(s) 361, 578, 748, 1729, 1735, 2051, 2096
AcuI CTGAAG 2 cut(s) 1074, 1430
AfaI GTAC 9 cut(s) 348, 472, 972, 1962, 2824, 3179, 3293, 3601, 3658
AfeI AGCGCT 1 cut(s) 728
AfiI CCNNNNNNNGG 7 cut(s) 125, 449, 709, 2148, 2191, 2957, 3744
AflIII ACRYGT 1 cut(s) 3820
AhdI GACNNNNNGTC 1 cut(s) 2859
AhlI ACTAGT 2 cut(s) 758, 1667
AjiI CACGTC 1 cut(s) 2810
AjnI CCWGG 6 cut(s) 189, 679, 1014, 3082, 3127, 3683
AjuI GAANNNNNNNTTGG 4 cut(s) 2627, 2659, 3659, 3691
Alw21I GWGCWC 3 cut(s) 919, 1464, 3801
AlwI GGATC 6 cut(s) 1552, 1752, 2609, 2634, 2647, 3198
AlwNI CAGNNNCTG 4 cut(s) 238, 371, 770, 1136
Ama87I CYCGRG 1 cut(s) 380
Aor51HI AGCGCT 1 cut(s) 728
ApeKI GCWGC 7 cut(s) 1370, 2133, 2246, 3065, 3259, 3281, 3320
ApoI RAATTY 7 cut(s) 361, 578, 748, 1729, 1735, 2051, 2096
AseI ATTAAT 2 cut(s) 1713, 2156
Asp700I GAANNNNTTC 2 cut(s) 331, 579
AspLEI GCGC 3 cut(s) 729, 1517, 1912
AspS9I GGNCC 9 cut(s) 116, 226, 532, 1012, 1196, 2182, 2671, 2960, 3854
AsuC2I CCSGG 2 cut(s) 1042, 2192
AsuHPI GGTGA 6 cut(s) 329, 566, 578, 653, 1520, 1563
AsuII TTCGAA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 6 cut(s) 116, 226, 1196, 2182, 2671, 2960
AxyI CCTNAGG 2 cut(s) 900, 2814
BalI TGGCCA 1 cut(s) 2781
BamHI GGATCC 1 cut(s) 2639
BanI GGYRCC 2 cut(s) 2578, 2744
BauI CACGAG 2 cut(s) 1875, 3557
BbsI GAAGAC 1 cut(s) 3579
Bbv12I GWGCWC 3 cut(s) 919, 1464, 3801
BbvCI CCTCAGC 1 cut(s) 2186
BbvI GCAGC 7 cut(s) 1357, 2145, 2233, 3052, 3268, 3271, 3307
BceAI ACGGC 2 cut(s) 109, 3683
BciT130I CCWGG 6 cut(s) 191, 681, 1016, 3084, 3129, 3685
BciVI GTATCC 1 cut(s) 1034
BclI TGATCA 3 cut(s) 925, 2560, 3169
BcnI CCSGG 2 cut(s) 1042, 2192
BcuI ACTAGT 2 cut(s) 758, 1667
BfaI CTAG 7 cut(s) 113, 759, 1034, 1668, 2619, 3305, 3431
BfmI CTRYAG 3 cut(s) 410, 1371, 3398
BfoI RGCGCY 1 cut(s) 730
BfuAI ACCTGC 1 cut(s) 3006
BfuI GTATCC 1 cut(s) 1034
BglII AGATCT 2 cut(s) 892, 3781
BisI GCNGC 9 cut(s) 1209, 1371, 2134, 2247, 2250, 3066, 3260, 3282, 3321
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 9 cut(s) 1210, 1372, 2135, 2248, 2251, 3067, 3261, 3283, 3322
Bme1390I CCNGG 8 cut(s) 191, 681, 1016, 1042, 2192, 3084, 3129, 3685
Bme18I GGWCC 6 cut(s) 116, 226, 1196, 2182, 2671, 2960
BmeRI GACNNNNNGTC 1 cut(s) 2859
BmeT110I CYCGRG 1 cut(s) 380
BmgBI CACGTC 1 cut(s) 2810
BmgT120I GGNCC 9 cut(s) 116, 226, 532, 1012, 1196, 2182, 2671, 2960, 3854
BmiI GGNNCC 5 cut(s) 1197, 2580, 2641, 2746, 2961
BmrFI CCNGG 8 cut(s) 191, 681, 1016, 1042, 2192, 3084, 3129, 3685
BmrI ACTGGG 2 cut(s) 248, 1019
BmsI GCATC 5 cut(s) 358, 802, 1315, 1501, 1516
BmuI ACTGGG 2 cut(s) 248, 1019
BoxI GACNNNNGTC 1 cut(s) 2676
BpiI GAAGAC 1 cut(s) 3579
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 5 cut(s) 132, 477, 702, 1686, 3828
Bpu10I CCTNAGC 1 cut(s) 2186
Bpu1102I GCTNAGC 1 cut(s) 723
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 3121, 3215
BpuMI CCSGG 2 cut(s) 1042, 2192
Bsa29I ATCGAT 1 cut(s) 3156
BsaAI YACGTR 1 cut(s) 3226
BsaBI GATNNNNATC 1 cut(s) 1437
BsaI GGTCTC 3 cut(s) 1950, 2098, 2282
BsaJI CCNNGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 2454, 2484
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 449, 709, 2148, 2191, 2957, 3744
Bse21I CCTNAGG 2 cut(s) 900, 2814
Bse3DI GCAATG 3 cut(s) 2030, 2582, 3707
Bse8I GATNNNNATC 1 cut(s) 1437
BseBI CCWGG 6 cut(s) 191, 681, 1016, 3084, 3129, 3685
BseCI ATCGAT 1 cut(s) 3156
BseDI CCNNGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
BseGI GGATG 8 cut(s) 228, 636, 817, 1024, 1095, 1567, 3205, 3426
BseJI GATNNNNATC 1 cut(s) 1437
BseLI CCNNNNNNNGG 7 cut(s) 125, 449, 709, 2148, 2191, 2957, 3744
BseMI GCAATG 3 cut(s) 2030, 2582, 3707
BseRI GAGGAG 1 cut(s) 2081
BseXI GCAGC 7 cut(s) 1357, 2145, 2233, 3052, 3268, 3271, 3307
BseYI CCCAGC 1 cut(s) 1191
BsgI GTGCAG 2 cut(s) 783, 3426
BshNI GGYRCC 2 cut(s) 2578, 2744
BshVI ATCGAT 1 cut(s) 3156
BsiHKAI GWGCWC 3 cut(s) 919, 1464, 3801
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 2 cut(s) 1041, 2191
BslFI GGGAC 2 cut(s) 1704, 2788
BslI CCNNNNNNNGG 7 cut(s) 125, 449, 709, 2148, 2191, 2957, 3744
BsmBI CGTCTC 2 cut(s) 3528, 3706
BsmFI GGGAC 2 cut(s) 1704, 2788
BsmI GAATGC 3 cut(s) 1211, 1314, 3043
Bso31I GGTCTC 3 cut(s) 1950, 2098, 2282
BsoBI CYCGRG 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 3 cut(s) 919, 1464, 3801
Bsp1407I TGTACA 2 cut(s) 346, 3291
Bsp1720I GCTNAGC 1 cut(s) 723
Bsp19I CCATGG 3 cut(s) 2292, 2674, 3108
BspACI CCGC 6 cut(s) 119, 664, 1209, 2250, 2708, 2970
BspDI ATCGAT 1 cut(s) 3156
BspHI TCATGA 1 cut(s) 3373
BspLI GGNNCC 5 cut(s) 1197, 2580, 2641, 2746, 2961
BspMAI CTGCAG 2 cut(s) 414, 1375
BspMI ACCTGC 1 cut(s) 3006
BspPI GGATC 6 cut(s) 1552, 1752, 2609, 2634, 2647, 3198
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 2 cut(s) 2578, 2744
BspTNI GGTCTC 3 cut(s) 1950, 2098, 2282
BsrDI GCAATG 3 cut(s) 2030, 2582, 3707
BsrGI TGTACA 2 cut(s) 346, 3291
BssECI CCNNGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
BssSI CACGAG 2 cut(s) 1875, 3557
BssT1I CCWWGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
Bst2BI CACGAG 2 cut(s) 1875, 3557
Bst2UI CCWGG 6 cut(s) 191, 681, 1016, 3084, 3129, 3685
Bst4CI ACNGT 7 cut(s) 43, 429, 1802, 1965, 2486, 2536, 3334
Bst6I CTCTTC 3 cut(s) 970, 3236, 3785
BstAPI GCANNNNNTGC 1 cut(s) 770
BstAUI TGTACA 2 cut(s) 346, 3291
BstBAI YACGTR 1 cut(s) 3226
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 8 cut(s) 600, 983, 1206, 1248, 2043, 2073, 2749, 3714
BstDSI CCRYGG 3 cut(s) 2292, 2674, 3108
BstENI CCTNNNNNAGG 1 cut(s) 3742
BstF5I GGATG 8 cut(s) 228, 636, 817, 1024, 1095, 1567, 3205, 3426
BstH2I RGCGCY 1 cut(s) 730
BstHHI GCGC 3 cut(s) 729, 1517, 1912
BstNI CCWGG 6 cut(s) 191, 681, 1016, 3084, 3129, 3685
BstNSI RCATGY 4 cut(s) 602, 1476, 3371, 3824
BstPAI GACNNNNGTC 1 cut(s) 2676
BstSCI CCNGG 8 cut(s) 189, 679, 1014, 1040, 2190, 3082, 3127, 3683
BstSFI CTRYAG 3 cut(s) 410, 1371, 3398
BstV1I GCAGC 7 cut(s) 1357, 2145, 2233, 3052, 3268, 3271, 3307
BstV2I GAAGAC 1 cut(s) 3579
BstX2I RGATCY 5 cut(s) 892, 1544, 2639, 3190, 3781
BstXI CCANNNNNNTGG 1 cut(s) 3007
BstYI RGATCY 5 cut(s) 892, 1544, 2639, 3190, 3781
Bsu15I ATCGAT 1 cut(s) 3156
Bsu36I CCTNAGG 2 cut(s) 900, 2814
BsuI GTATCC 1 cut(s) 1034
BsuTUI ATCGAT 1 cut(s) 3156
BtgI CCRYGG 3 cut(s) 2292, 2674, 3108
BtrI CACGTC 1 cut(s) 2810
BtsCI GGATG 8 cut(s) 228, 636, 817, 1024, 1095, 1567, 3205, 3426
BtsI GCAGTG 1 cut(s) 3570
BveI ACCTGC 1 cut(s) 3006
Cac8I GCNNGC 8 cut(s) 600, 983, 1206, 1248, 2043, 2073, 2749, 3714
CaiI CAGNNNCTG 4 cut(s) 238, 371, 770, 1136
CciI TCATGA 1 cut(s) 3373
CfoI GCGC 3 cut(s) 729, 1517, 1912
Cfr13I GGNCC 9 cut(s) 116, 226, 532, 1012, 1196, 2182, 2671, 2960, 3854
ClaI ATCGAT 1 cut(s) 3156
CseI GACGC 2 cut(s) 1406, 2024
Csp6I GTAC 9 cut(s) 347, 471, 971, 1961, 2823, 3178, 3292, 3600, 3657
CviQI GTAC 9 cut(s) 347, 471, 971, 1961, 2823, 3178, 3292, 3600, 3657
DraI TTTAAA 5 cut(s) 400, 625, 2667, 2740, 3057
DrdI GACNNNNNNGTC 1 cut(s) 28
DriI GACNNNNNGTC 1 cut(s) 2859
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 1206, 2779
Eam1104I CTCTTC 3 cut(s) 970, 3236, 3785
Eam1105I GACNNNNNGTC 1 cut(s) 2859
EarI CTCTTC 3 cut(s) 970, 3236, 3785
EciI GGCGGA 1 cut(s) 108
Eco130I CCWWGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
Eco147I AGGCCT 4 cut(s) 1579, 2466, 2902, 3737
Eco31I GGTCTC 3 cut(s) 1950, 2098, 2282
Eco32I GATATC 2 cut(s) 2922, 3154
Eco47I GGWCC 6 cut(s) 116, 226, 1196, 2182, 2671, 2960
Eco47III AGCGCT 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 1074, 1430
Eco81I CCTNAGG 2 cut(s) 900, 2814
Eco88I CYCGRG 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 3742
EcoO109I RGGNCCY 2 cut(s) 1196, 2960
EcoRII CCWGG 6 cut(s) 189, 679, 1014, 3082, 3127, 3683
EcoRV GATATC 2 cut(s) 2922, 3154
EcoT14I CCWWGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
EcoT22I ATGCAT 2 cut(s) 600, 1330
ErhI CCWWGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
Esp3I CGTCTC 2 cut(s) 3528, 3706
FalI AAGNNNNNCTT 4 cut(s) 2451, 2483, 2627, 2659
FaqI GGGAC 2 cut(s) 1704, 2788
FauI CCCGC 1 cut(s) 657
FauNDI CATATG 1 cut(s) 1657
FbaI TGATCA 3 cut(s) 925, 2560, 3169
Fnu4HI GCNGC 9 cut(s) 1209, 1371, 2134, 2247, 2250, 3066, 3260, 3282, 3321
FokI GGATG 8 cut(s) 235, 643, 824, 1031, 1082, 1574, 3212, 3413
Fsp4HI GCNGC 9 cut(s) 1209, 1371, 2134, 2247, 2250, 3066, 3260, 3282, 3321
FspBI CTAG 7 cut(s) 113, 759, 1034, 1668, 2619, 3305, 3431
GlaI GCGC 3 cut(s) 728, 1516, 1911
GluI GCNGC 9 cut(s) 1209, 1371, 2134, 2247, 2250, 3066, 3260, 3282, 3321
GsaI CCCAGC 1 cut(s) 1195
GsuI CTGGAG 5 cut(s) 132, 477, 702, 1686, 3828
HaeII RGCGCY 1 cut(s) 730
HapII CCGG 2 cut(s) 1041, 2191
HgaI GACGC 2 cut(s) 1406, 2024
HhaI GCGC 3 cut(s) 729, 1517, 1912
Hin6I GCGC 3 cut(s) 727, 1515, 1910
HinP1I GCGC 3 cut(s) 727, 1515, 1910
HincII GTYRAC 3 cut(s) 33, 91, 196
HindII GTYRAC 3 cut(s) 33, 91, 196
HindIII AAGCTT 1 cut(s) 1792
HpaI GTTAAC 1 cut(s) 196
HpaII CCGG 2 cut(s) 1041, 2191
HphI GGTGA 6 cut(s) 329, 566, 578, 653, 1520, 1563
Hpy166II GTNNAC 6 cut(s) 33, 91, 196, 1353, 1396, 2473
Hpy8I GTNNAC 6 cut(s) 33, 91, 196, 1353, 1396, 2473
Hpy99I CGWCG 1 cut(s) 2857
HpyCH4III ACNGT 7 cut(s) 43, 429, 1802, 1965, 2486, 2536, 3334
HpyCH4IV ACGT 3 cut(s) 2809, 3225, 3753
HpySE526I ACGT 3 cut(s) 2809, 3225, 3753
HspAI GCGC 3 cut(s) 727, 1515, 1910
Ksp22I TGATCA 3 cut(s) 925, 2560, 3169
KspAI GTTAAC 1 cut(s) 196
LmnI GCTCC 3 cut(s) 215, 1459, 2068
Lsp1109I GCAGC 7 cut(s) 1357, 2145, 2233, 3052, 3268, 3271, 3307
LweI GCATC 5 cut(s) 358, 802, 1315, 1501, 1516
MaeI CTAG 7 cut(s) 113, 759, 1034, 1668, 2619, 3305, 3431
MaeII ACGT 3 cut(s) 2809, 3225, 3753
MaeIII GTNAC 8 cut(s) 272, 373, 554, 1036, 1150, 1219, 1291, 1333
MfeI CAATTG 3 cut(s) 302, 1419, 1499
MflI RGATCY 5 cut(s) 892, 1544, 2639, 3190, 3781
MhlI GDGCHC 3 cut(s) 919, 1464, 3801
MlsI TGGCCA 1 cut(s) 2781
MluNI TGGCCA 1 cut(s) 2781
MlyI GAGTC 5 cut(s) 239, 956, 1142, 1216, 3253
MmeI TCCRAC 1 cut(s) 1889
Mox20I TGGCCA 1 cut(s) 2781
Mph1103I ATGCAT 2 cut(s) 600, 1330
MroXI GAANNNNTTC 2 cut(s) 331, 579
MscI TGGCCA 1 cut(s) 2781
MslI CAYNNNNRTG 4 cut(s) 603, 1340, 2768, 3372
Msp20I TGGCCA 1 cut(s) 2781
MspA1I CMGCKG 3 cut(s) 1628, 2252, 3320
MspI CCGG 2 cut(s) 1041, 2191
MspR9I CCNGG 8 cut(s) 191, 681, 1016, 1042, 2192, 3084, 3129, 3685
MunI CAATTG 3 cut(s) 302, 1419, 1499
Mva1269I GAATGC 3 cut(s) 1211, 1314, 3043
MvaI CCWGG 6 cut(s) 191, 681, 1016, 3084, 3129, 3685
NciI CCSGG 2 cut(s) 1042, 2192
NcoI CCATGG 3 cut(s) 2292, 2674, 3108
NdeI CATATG 1 cut(s) 1657
NlaIV GGNNCC 5 cut(s) 1197, 2580, 2641, 2746, 2961
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 5 cut(s) 272, 554, 1150, 1219, 1333
NsiI ATGCAT 2 cut(s) 600, 1330
NspI RCATGY 4 cut(s) 602, 1476, 3371, 3824
NspV TTCGAA 1 cut(s) 68
PaeI GCATGC 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 380
PagI TCATGA 1 cut(s) 3373
PceI AGGCCT 4 cut(s) 1579, 2466, 2902, 3737
PciI ACATGT 1 cut(s) 3820
PcsI WCGNNNNNNNCGW 2 cut(s) 868, 2849
PctI GAATGC 3 cut(s) 1211, 1314, 3043
PdmI GAANNNNTTC 2 cut(s) 331, 579
PkrI GCNGC 9 cut(s) 1210, 1372, 2135, 2248, 2251, 3067, 3261, 3283, 3322
PleI GAGTC 5 cut(s) 239, 956, 1141, 1216, 3252
PpsI GAGTC 5 cut(s) 239, 956, 1141, 1216, 3252
Ppu21I YACGTR 1 cut(s) 3226
PpuMI RGGWCCY 2 cut(s) 1196, 2960
PscI ACATGT 1 cut(s) 3820
PshAI GACNNNNGTC 1 cut(s) 2676
PshBI ATTAAT 2 cut(s) 1713, 2156
PsiI TTATAA 1 cut(s) 1088
Psp5II RGGWCCY 2 cut(s) 1196, 2960
Psp6I CCWGG 6 cut(s) 189, 679, 1014, 3082, 3127, 3683
PspFI CCCAGC 1 cut(s) 1191
PspGI CCWGG 6 cut(s) 189, 679, 1014, 3082, 3127, 3683
PspN4I GGNNCC 5 cut(s) 1197, 2580, 2641, 2746, 2961
PspPI GGNCC 9 cut(s) 116, 226, 532, 1012, 1196, 2182, 2671, 2960, 3854
PspPPI RGGWCCY 2 cut(s) 1196, 2960
PspXI VCTCGAGB 1 cut(s) 380
PstI CTGCAG 2 cut(s) 414, 1375
PstNI CAGNNNCTG 4 cut(s) 238, 371, 770, 1136
PsuI RGATCY 5 cut(s) 892, 1544, 2639, 3190, 3781
PvuII CAGCTG 2 cut(s) 1628, 3320
RsaI GTAC 9 cut(s) 348, 472, 972, 1962, 2824, 3179, 3293, 3601, 3658
RsaNI GTAC 9 cut(s) 347, 471, 971, 1961, 2823, 3178, 3292, 3600, 3657
RseI CAYNNNNRTG 4 cut(s) 603, 1340, 2768, 3372
SatI GCNGC 9 cut(s) 1209, 1371, 2134, 2247, 2250, 3066, 3260, 3282, 3321
Sau96I GGNCC 9 cut(s) 116, 226, 532, 1012, 1196, 2182, 2671, 2960, 3854
SchI GAGTC 5 cut(s) 239, 956, 1142, 1216, 3253
ScrFI CCNGG 8 cut(s) 191, 681, 1016, 1042, 2192, 3084, 3129, 3685
SduI GDGCHC 3 cut(s) 919, 1464, 3801
SfaNI GCATC 5 cut(s) 358, 802, 1315, 1501, 1516
SfcI CTRYAG 3 cut(s) 410, 1371, 3398
Sfr274I CTCGAG 1 cut(s) 380
SfuI TTCGAA 1 cut(s) 68
SinI GGWCC 6 cut(s) 116, 226, 1196, 2182, 2671, 2960
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 4 cut(s) 603, 1340, 2768, 3372
SmlI CTYRAG 3 cut(s) 380, 3100, 3194
SmoI CTYRAG 3 cut(s) 380, 3100, 3194
SpeI ACTAGT 2 cut(s) 758, 1667
SphI GCATGC 1 cut(s) 602
SseBI AGGCCT 4 cut(s) 1579, 2466, 2902, 3737
SsiI CCGC 6 cut(s) 119, 664, 1209, 2250, 2708, 2970
SspI AATATT 2 cut(s) 2016, 2315
SspMI CTAG 7 cut(s) 113, 759, 1034, 1668, 2619, 3305, 3431
StuI AGGCCT 4 cut(s) 1579, 2466, 2902, 3737
StyD4I CCNGG 8 cut(s) 189, 679, 1014, 1040, 2190, 3082, 3127, 3683
StyI CCWWGG 7 cut(s) 447, 846, 1574, 2292, 2643, 2674, 3108
TaaI ACNGT 7 cut(s) 43, 429, 1802, 1965, 2486, 2536, 3334
TaiI ACGT 3 cut(s) 2812, 3228, 3756
TaqI TCGA 9 cut(s) 68, 131, 381, 871, 1276, 1428, 1902, 1948, 3156
TaqII GACCGA 2 cut(s) 243, 717
TatI WGTACW 5 cut(s) 346, 1960, 2822, 3291, 3599
TauI GCSGC 2 cut(s) 1211, 2252
TseFI GTSAC 5 cut(s) 272, 554, 1150, 1219, 1333
TseI GCWGC 7 cut(s) 1370, 2133, 2246, 3065, 3259, 3281, 3320
Tsp45I GTSAC 5 cut(s) 272, 554, 1150, 1219, 1333
TspGWI ACGGA 4 cut(s) 824, 2345, 2870, 3510
VpaK11BI GGWCC 6 cut(s) 116, 226, 1196, 2182, 2671, 2960
VspI ATTAAT 2 cut(s) 1713, 2156
XagI CCTNNNNNAGG 1 cut(s) 3742
XapI RAATTY 7 cut(s) 361, 578, 748, 1729, 1735, 2051, 2096
XceI RCATGY 4 cut(s) 602, 1476, 3371, 3824
XcmI CCANNNNNNNNNTGG 1 cut(s) 3636
XhoI CTCGAG 1 cut(s) 380
XmnI GAANNNNTTC 2 cut(s) 331, 579
XspI CTAG 7 cut(s) 113, 759, 1034, 1668, 2619, 3305, 3431
Zsp2I ATGCAT 2 cut(s) 600, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.