Rroxscaffold_7G00187190

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002933
Physical Location & Seq
Reverse (-)
26581168 .. 26593939
12772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187190.1

Sequence Viewer

Length: 4503 bp
ATGGTCATCGTCTTCACCAGAGTGGAGGCCGATGCTATGTACGTGACCTTTTCCAGGGTTTCGGTCAGCGAATTGTTGGAATTTACTGATAAGAATCTGAATGAGGGGAGCTCCATTATGTTCTGCCAGAATTTCATCAATGAGGTTATGGAAGCTAGTTATGGGGGTCCTGATGGTATCAAAATGGCGGCTCCGGCCCCCAGGAGATTGCAAAATGGAAATGCCGCCACTGGACGCGGTGAGTCGGGGGCTGGAGAGTCCAATGATGAAGCTGAATCTGGCGAGGGAGGTGGTAGTAGTAAGATTCGGGATGATGGATTTCTTTTGTTTAAGAATTTGTGTAAGTTGTCGATGAAGTTCTCGTCACAGGAGTACTCTGATGATCAAATACTATTGAGGGGTAAAATATTGTCGTTGGAGCTTCTGAAGGTGGTTATGGACAATGGGGGTCCTATTTGGCTCTCTAATGACAGGTTTCTCAATGGCATCAAGCAGTTCCTATGCTTATCATTGCTAAAAAACAGTGCGCTGTCAGTTATGGCTATTTTCCAGCTCCAGTGTTCTATTTTCACGAGCTTGTTATCAAAATTTAGATCAGGGTTGAAAGCAGAAATCGGTATATTTTTTCCCATGCTCGTCTTACGCGTGCTTGAGAATGTTCTCCAGCCTAGTTTCTTGCAGAAAATGACGGTCCTCAATTTATTAGAGAAGATCTCTCAGGATCCACAGATTATCATTGACATTTTTGTCAATTATGACTGTGATGTGGATTCTCCAAACATTTTCGAAAGGATTGTCAACGGCCTTCTGAAAACTGCTCTAGGTCCACCTTCTGGTTCAACAACAACATTATCTCCAGTTCAGGATATTACTTTCCGGCATGAATCAGTGAAGTGCTTGGTTAACATAATTAAGTCAATGGGAGCTTGGATGGACCGACAGAGACTGGGAGACTCCTATCTGCCAAATACCAATGAGAGTGACACTACATCAGAGAAAACAGAGAATCAATTGATCCTAAATGGTGAAGAAGGAACCGCTTTAGACAATGAAGTACATCCAGAAGGAAGTTCAGATGCTGCAACTCTCGAGCAACGTCGAGCTTTTAAACTTGAACTGCAGAAAGGTATCTCACTGTTCAATAGGAAACCTTCCAAGGGCATTGAGTTTCTGATAAGCACCAAAAAAATTGGGGGTTCTCCAGCAGATGTGGCTTCTTTCCTGAAGAACAACACTGCGGGCCTAAATGAAACCATGATCGGTGACTATTTGGGTGAAAGGGAAGAATTTTCTCTAAAAGTTATGCATGCTTATGTAGATTCCTTTAATTTCAAAGGGATGGATTTTGGTGAAGCAATTAGTCCGAATTCATTTACTAGTGCAGATACTGCTTATGTCCTTGCATACTCTGTGATAATGCTCAACACGGATGCCCATAATATCATGGTTAAAGATAAGATGACCAAGGCCGATTTCATACGTAACAACCGAGGAATAGATGATGGCAAGGATCTACCTGAGGAATATCTTGGTGCACTATATGATCAGATTGTTAAAAATGAAATTAAGATGAAGGCTGACTCTTCTGTACCACAGAGCAAGCAGGAAAACAGCTTTAACAAATTATTGGGCCTGGATGGTATCCTGAATCTAGTAACTGGGAAGCAGACTGAAGAAAAAGCGTTGGGTGCAAATGGGCTTCTTATTAAGGACATCCAAGAGCAATTTAAGGCGAAGTCAGGGAAATCAGAGTCTGTTTATCATTCTGTGACAGATGTGGCCATATTGAGGTTCATGGTGGAGGCCTGCTGGGGTCCTATGCTGGCCGCATTCAGTGTGACTCTGGACCAAAGTGATGACAGGCTTGCTACTGCTCAATGCTTACTGGGTTTCCGACATGCAATTCATGTTACTGCTCTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACATCAATGGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAGAATGTTGACGCTGTGAAGGCAATAATAGCAATCGCCGTTGAAGATGGTAATCATCTTCAGGAAGCATGGGAGCACATATTAACATGCCTTTCCCGAATTGAGCATCTGCAACTGTTGGGTGAGGGCGCACCTACTGATGCAACCTTTTTCAGTGGATCTAATGTTGAAGCGGATGACAAATCACCAAAGCCTATTGGTTTTGCTTCTCTAAAGAAAAAAGGAACTATCCAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGATAGCACTAGTATTGGAATCAACACTTCGGGACTGGTATCTCCAGAACAGATTAATAATTTTATTTCGAATTTGAATTTGCTGGATCAGATTGGGAATTTTGAGTTGAACCATGTATTTGCTCATAGCCAAAGCTTGAACAGTGAAGCCATAGTTGCTTTTGTGAAGTCCTTATGTAAAGTTTCCATGTCAGAATTGCAGTCTCCAACAGATCCTCGTGTTTTCAGCCTCACAAAAATAGTTGAAATAGCGCATTATAATATGAACCGCATCAGATTAGTGTGGTCTCGAATATGGAATGTACTGTCAGATTTCTTTGTATCAGTTGGCTTGTCAGAGAATCTTTCAGTTGCAATATTTGTGATGGATTCCTTGCGTCAGCTTGCTATGAAATTCTTAGAGCGTGAGGAGCTGGCAAATTACAATTTCCAGAATGAATTTTTAAGACCATTTGTGATTGTTATGCAGAAAAGCAGCTCTACAGAAATTAGGGAATTAATAGTTAGGTGCATTTCACAAATGGTCCTCAGCCGGGTCAATAATGTGAAATCTGGTTGGAAAAGTGTTTTTATGGTTTTCACAATTGCTGCTGCTGATGAGAGGAAAAATATAGTCTTGTTGGCCTTTGAGACCATGGAAAAGATAGTCAGAGAGTATTTCCCTTACATAACTGAGACAGAGACATTGACTTTTACTGATTGTGTTAGATGCCTCTTAACCTTCACAAATAGCAGATTCAATAGTGATGTTAGCCTCAATGCTATTGCGTTTCTGCGTTTCTGTGCCGTCAAACTTGCAGAAGGAGGCCTTGTTTACAATAAGAACAGTGAGATTGATGGTTCTTCAAGTCCTAGCGCAAGTGAAGGTGCTTCAACTGTTGAGAATTTCAATGATAAAGATGATCATACATCATTTTGGGTTCCCTTGCTAACAGGTTTGTCCAAACTAACATCTGATCCTAGATCAGCTATCAGGAAAGGATCCTTGGAAGTTCTTTTCAACATTCTAAAGGACCATGGTCATCTTTTCTCACACCTGTTTTGGACTGCGGTTTTCAATTCTGTTATTTTTCCCATATTTAATGGTGTCAACGATAAGAAAGATACAGACATGAAAAATGACCAGTCCTCGCCAATTTCGATGTCCCCACGTCCTGAAGGAAGTACATGGGATTCTGAAACTTCAGCTGTTGCGGCGGATTGCCTCATAGATCTGTTCGTCAGTTTCTTTGATGTTGTTAGGTCTCAACTACCAAGTGTGATATCCATTCTGACAGGACTCATCAGAAGTCCCATTCAGGGTCCTGCTACTGCAGGAGTTGCTGCATTAATGCGTTTGTCTAGTGAGGTGGGTAGCAGGCTTTCAGAAGATGAATGGAGAGCAATTTTTCTGGCTTTAAAGGAGGCTGCCACATCTGCAGTGCCTGGATTTTTGAAGGTCTTGAGAACCATGGATAATGTCAATGTTCCTGGTCTTTCTGAATCTTTTAGTGATATCGATATGTTGTCTGATCAGGGGTACTCAAATGAGGATCTTGAGGATGATAATTTGCAAACAGCTTCATATGTGGTTTCAAGAATCAAGAGTCATGTTTCTATGCAGCTACTCATCTTACAGGTCGCTGCTGATTTGTACAAAATCAACCTAGAAACCTTGTCAGCTGCTAACATTGCAGTTCTTCTTGAAGTATATTCTCTCGTGGCATCGCATGCTCACCAACTGAACTCTGAGACAATTCTGCAGAAGAAGCTGGAAAAAGTGTGTTCCATCCTAGAACTCACTGCACCACCTATTGTTCATTTTGAGAATGAATCTTACAAAAACTATCTTAACTTCCTCCAAAACGCACTTGTGGATAATCCATCTCTTTCCAAGGAAATGGACATAGAAGCTAAACTCGTGGCAGTCTGTGAAAACATATTGCAGATATACCTAAAGTGTACTGAGCTTCAATCTTCTGAGCAGAAGCCTACTGGTCAGCCAGTTTTGCACTGGATTCTTCCTTTGGGTACAGCAAAAAAGGAGGAATTGGCTACCAGGACTTTCTTAGCCGTCTCGGCATTGCAGGCATTGAGTGGTTTGGAAAAGGTTTCATTTAGGAGGCACGTTTCTCAGTTATTTCCTCTGTTGGTAGATCTTGTGAAGAGTGAGCACACTTCTGGAGAAGTTCAACACGTTCTAAGCAACATATTCCAGTCATGTATAGGCCCAATAATAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1500

Amino Acids

165.91

Weight (kDa)

5.26

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 103 - 261 2.3e-42 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 370 - 453 1.3e-22 Sec7 domain
Sec7 PF01369 455 - 522 1.5e-25 Sec7 domain
Sec7-like_HDS PF09324 890 - 972 2.1e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1062 - 1129 3.6e-06 C-terminal region of Mon2 protein
BIG2_C PF20252 1292 - 1491 1.6e-26 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2535
AasI GACNNNNNNGTC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 833
AccII CGCG 2 cut(s) 237, 645
AcoI YGGCCR 2 cut(s) 1779, 1824
AcuI CTGAAG 6 cut(s) 446, 1244, 1692, 2048, 3444, 3453
AfiI CCNNNNNNNGG 6 cut(s) 54, 833, 1157, 1788, 2809, 3575
AflIII ACRYGT 2 cut(s) 643, 4450
AhlI ACTAGT 2 cut(s) 1376, 2285
AjiI CACGTC 1 cut(s) 3428
AjnI CCWGG 6 cut(s) 53, 200, 1632, 3700, 3745, 4313
AjuI GAANNNNNNNTTGG 4 cut(s) 1457, 1489, 3245, 3277
AleI CACNNNNGTG 1 cut(s) 20
Alw21I GWGCWC 4 cut(s) 113, 1537, 2082, 4431
Alw44I GTGCAC 1 cut(s) 1533
AlwNI CAGNNNCTG 5 cut(s) 946, 1079, 1388, 1754, 3701
Ama87I CYCGRG 1 cut(s) 1088
ApaLI GTGCAC 1 cut(s) 1533
ArsI GACNNNNNNTTYG 2 cut(s) 780, 812
AseI ATTAAT 3 cut(s) 2331, 2774, 3605
AspLEI GCGC 4 cut(s) 529, 2135, 2530, 3134
AsuC2I CCSGG 1 cut(s) 2810
AsuHPI GGTGA 9 cut(s) 7, 251, 1037, 1274, 1286, 1361, 2138, 2181, 3983
AsuII TTCGAA 2 cut(s) 786, 2345
AvaI CYCGRG 1 cut(s) 1088
AxyI CCTNAGG 1 cut(s) 1518
BaeGI GKGCMC 1 cut(s) 1537
BalI TGGCCA 1 cut(s) 1781
BamHI GGATCC 2 cut(s) 721, 3257
BanII GRGCYC 1 cut(s) 113
BauI CACGAG 4 cut(s) 571, 2493, 3974, 4175
BbsI GAAGAC 1 cut(s) 4
Bbv12I GWGCWC 4 cut(s) 113, 1537, 2082, 4431
BbvCI CCTCAGC 1 cut(s) 2804
BceAI ACGGC 4 cut(s) 817, 2027, 3047, 4313
BciT130I CCWGG 6 cut(s) 55, 202, 1634, 3702, 3747, 4315
BciVI GTATCC 1 cut(s) 1652
BclI TGATCA 4 cut(s) 382, 1543, 3178, 3787
BcnI CCSGG 1 cut(s) 2810
BcuI ACTAGT 2 cut(s) 1376, 2285
BfmI CTRYAG 6 cut(s) 1118, 1989, 2757, 3588, 3693, 4016
BfuI GTATCC 1 cut(s) 1652
BglI GCCNNNNNGGC 1 cut(s) 4334
BglII AGATCT 3 cut(s) 711, 3487, 4411
BmcAI AGTACT 1 cut(s) 374
Bme1390I CCNGG 7 cut(s) 55, 202, 1634, 2810, 3702, 3747, 4315
BmeT110I CYCGRG 1 cut(s) 1088
BmgBI CACGTC 1 cut(s) 3428
BmrFI CCNGG 7 cut(s) 55, 202, 1634, 2810, 3702, 3747, 4315
BmrI ACTGGG 3 cut(s) 956, 1668, 1895
BmuI ACTGGG 3 cut(s) 956, 1668, 1895
BoxI GACNNNNGTC 1 cut(s) 3294
BpiI GAAGAC 1 cut(s) 4
BplI GAGNNNNNCTC 4 cut(s) 95, 127, 698, 730
BpmI CTGGAG 7 cut(s) 273, 539, 647, 840, 1185, 2304, 4458
Bpu10I CCTNAGC 1 cut(s) 2804
Bpu14I TTCGAA 2 cut(s) 786, 2345
BpuEI CTTGAG 3 cut(s) 671, 3739, 3833
BpuMI CCSGG 1 cut(s) 2810
Bsa29I ATCGAT 1 cut(s) 3774
BsaAI YACGTR 2 cut(s) 43, 1481
BsaBI GATNNNNATC 2 cut(s) 93, 2055
BsaI GGTCTC 3 cut(s) 2568, 2900, 3525
BsaXI ACNNNNNCTCC 4 cut(s) 838, 868, 3072, 3102
Bsc4I CCNNNNNNNGG 6 cut(s) 54, 833, 1157, 1788, 2809, 3575
Bse21I CCTNAGG 1 cut(s) 1518
Bse3DI GCAATG 3 cut(s) 509, 3945, 4337
Bse8I GATNNNNATC 2 cut(s) 93, 2055
BseBI CCWGG 6 cut(s) 55, 202, 1634, 3702, 3747, 4315
BseCI ATCGAT 1 cut(s) 3774
BseJI GATNNNNATC 2 cut(s) 93, 2055
BseLI CCNNNNNNNGG 6 cut(s) 54, 833, 1157, 1788, 2809, 3575
BseMI GCAATG 3 cut(s) 509, 3945, 4337
BseMII CTCAG 8 cut(s) 731, 1509, 2818, 2940, 3996, 4212, 4227, 4403
BseRI GAGGAG 1 cut(s) 2699
BseSI GKGCMC 1 cut(s) 1537
BseYI CCCAGC 1 cut(s) 1809
BsgI GTGCAG 2 cut(s) 1401, 4044
Bsh1236I CGCG 2 cut(s) 237, 645
BshVI ATCGAT 1 cut(s) 3774
BsiHKAI GWGCWC 4 cut(s) 113, 1537, 2082, 4431
BsiHKCI CYCGRG 1 cut(s) 1088
BsiSI CCGG 3 cut(s) 194, 877, 2809
BslFI GGGAC 3 cut(s) 2322, 3406, 3552
BslI CCNNNNNNNGG 6 cut(s) 54, 833, 1157, 1788, 2809, 3575
BsmBI CGTCTC 1 cut(s) 4336
BsmFI GGGAC 3 cut(s) 2322, 3406, 3552
BsmI GAATGC 2 cut(s) 1829, 1932
Bso31I GGTCTC 3 cut(s) 2568, 2900, 3525
BsoBI CYCGRG 1 cut(s) 1088
Bsp119I TTCGAA 2 cut(s) 786, 2345
Bsp1286I GDGCHC 4 cut(s) 113, 1537, 2082, 4431
Bsp1407I TGTACA 1 cut(s) 3909
Bsp19I CCATGG 3 cut(s) 2910, 3292, 3726
BspCNI CTCAG 8 cut(s) 730, 1510, 2817, 2941, 3997, 4213, 4228, 4402
BspDI ATCGAT 1 cut(s) 3774
BspFNI CGCG 2 cut(s) 237, 645
BspMAI CTGCAG 5 cut(s) 1122, 1993, 3592, 3697, 4020
BspT104I TTCGAA 2 cut(s) 786, 2345
BspTNI GGTCTC 3 cut(s) 2568, 2900, 3525
BsrDI GCAATG 3 cut(s) 509, 3945, 4337
BsrGI TGTACA 1 cut(s) 3909
BssSI CACGAG 4 cut(s) 571, 2493, 3974, 4175
BssT1I CCWWGG 7 cut(s) 1155, 1464, 2910, 3261, 3292, 3726, 4149
Bst2BI CACGAG 4 cut(s) 571, 2493, 3974, 4175
Bst2UI CCWGG 6 cut(s) 55, 202, 1634, 3702, 3747, 4315
Bst4CI ACNGT 9 cut(s) 524, 691, 761, 1137, 2121, 2420, 2583, 3104, 3154
Bst6I CTCTTC 2 cut(s) 1588, 4415
BstAPI GCANNNNNTGC 3 cut(s) 1388, 3596, 3986
BstAUI TGTACA 1 cut(s) 3909
BstBAI YACGTR 2 cut(s) 43, 1481
BstBI TTCGAA 2 cut(s) 786, 2345
BstDSI CCRYGG 3 cut(s) 2910, 3292, 3726
BstENI CCTNNNNNAGG 1 cut(s) 52
BstFNI CGCG 2 cut(s) 237, 645
BstHHI GCGC 4 cut(s) 529, 2135, 2530, 3134
BstNI CCWGG 6 cut(s) 55, 202, 1634, 3702, 3747, 4315
BstNSI RCATGY 4 cut(s) 1310, 1901, 2094, 3989
BstPAI GACNNNNGTC 1 cut(s) 3294
BstSCI CCNGG 7 cut(s) 53, 200, 1632, 2808, 3700, 3745, 4313
BstSFI CTRYAG 6 cut(s) 1118, 1989, 2757, 3588, 3693, 4016
BstSLI GKGCMC 1 cut(s) 1537
BstSNI TACGTA 1 cut(s) 1481
BstUI CGCG 2 cut(s) 237, 645
BstV2I GAAGAC 1 cut(s) 4
BstX2I RGATCY 9 cut(s) 711, 721, 1510, 2162, 2488, 3257, 3487, 3808, 4411
BstXI CCANNNNNNTGG 1 cut(s) 4156
BstYI RGATCY 9 cut(s) 711, 721, 1510, 2162, 2488, 3257, 3487, 3808, 4411
Bsu15I ATCGAT 1 cut(s) 3774
Bsu36I CCTNAGG 1 cut(s) 1518
BsuI GTATCC 1 cut(s) 1652
BsuTUI ATCGAT 1 cut(s) 3774
BtgI CCRYGG 3 cut(s) 2910, 3292, 3726
BtgZI GCGATG 1 cut(s) 3966
BtrI CACGTC 1 cut(s) 3428
BtsI GCAGTG 3 cut(s) 1233, 3702, 4056
CaiI CAGNNNCTG 5 cut(s) 946, 1079, 1388, 1754, 3701
CfoI GCGC 4 cut(s) 529, 2135, 2530, 3134
ClaI ATCGAT 1 cut(s) 3774
CseI GACGC 3 cut(s) 243, 2024, 2642
DraI TTTAAA 2 cut(s) 1108, 3675
DrdI GACNNNNNNGTC 1 cut(s) 746
DseDI GACNNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 2 cut(s) 1779, 1824
Eam1104I CTCTTC 2 cut(s) 1588, 4415
EarI CTCTTC 2 cut(s) 1588, 4415
EciI GGCGGA 1 cut(s) 3488
Ecl136II GAGCTC 1 cut(s) 111
Eco105I TACGTA 1 cut(s) 1481
Eco130I CCWWGG 7 cut(s) 1155, 1464, 2910, 3261, 3292, 3726, 4149
Eco147I AGGCCT 2 cut(s) 1805, 3084
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 3 cut(s) 2568, 2900, 3525
Eco32I GATATC 2 cut(s) 3540, 3772
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 6 cut(s) 446, 1244, 1692, 2048, 3444, 3453
Eco81I CCTNAGG 1 cut(s) 1518
Eco88I CYCGRG 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 52
EcoO109I RGGNCCY 4 cut(s) 167, 449, 1814, 3578
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 6 cut(s) 53, 200, 1632, 3700, 3745, 4313
EcoRV GATATC 2 cut(s) 3540, 3772
EcoT14I CCWWGG 7 cut(s) 1155, 1464, 2910, 3261, 3292, 3726, 4149
EcoT22I ATGCAT 2 cut(s) 1308, 1948
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 7 cut(s) 1155, 1464, 2910, 3261, 3292, 3726, 4149
Esp3I CGTCTC 1 cut(s) 4336
FalI AAGNNNNNCTT 2 cut(s) 3069, 3101
FaqI GGGAC 3 cut(s) 2322, 3406, 3552
FauI CCCGC 1 cut(s) 1231
FauNDI CATATG 2 cut(s) 2275, 3841
FbaI TGATCA 4 cut(s) 382, 1543, 3178, 3787
FriOI GRGCYC 1 cut(s) 113
GlaI GCGC 4 cut(s) 528, 2134, 2529, 3133
GsaI CCCAGC 1 cut(s) 1813
GsuI CTGGAG 7 cut(s) 273, 539, 647, 840, 1185, 2304, 4458
HapII CCGG 3 cut(s) 194, 877, 2809
HgaI GACGC 3 cut(s) 243, 2024, 2642
HhaI GCGC 4 cut(s) 529, 2135, 2530, 3134
Hin6I GCGC 4 cut(s) 527, 2133, 2528, 3132
HinP1I GCGC 4 cut(s) 527, 2133, 2528, 3132
HincII GTYRAC 4 cut(s) 799, 904, 2014, 3367
HindII GTYRAC 4 cut(s) 799, 904, 2014, 3367
HindIII AAGCTT 1 cut(s) 2410
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 3 cut(s) 194, 877, 2809
HphI GGTGA 9 cut(s) 7, 251, 1037, 1274, 1286, 1361, 2138, 2181, 3983
Hpy166II GTNNAC 9 cut(s) 799, 827, 904, 1535, 1971, 2014, 3091, 3367, 4218
Hpy8I GTNNAC 9 cut(s) 799, 827, 904, 1535, 1971, 2014, 3091, 3367, 4218
Hpy99I CGWCG 1 cut(s) 1101
HpyCH4III ACNGT 9 cut(s) 524, 691, 761, 1137, 2121, 2420, 2583, 3104, 3154
HpyCH4IV ACGT 6 cut(s) 42, 1096, 1480, 3427, 4383, 4452
HpySE526I ACGT 6 cut(s) 42, 1096, 1480, 3427, 4383, 4452
HspAI GCGC 4 cut(s) 527, 2133, 2528, 3132
Ksp22I TGATCA 4 cut(s) 382, 1543, 3178, 3787
KspAI GTTAAC 1 cut(s) 904
LmnI GCTCC 8 cut(s) 108, 116, 196, 418, 558, 923, 2077, 2686
MaeII ACGT 6 cut(s) 42, 1096, 1480, 3427, 4383, 4452
MfeI CAATTG 2 cut(s) 1010, 2859
MflI RGATCY 9 cut(s) 711, 721, 1510, 2162, 2488, 3257, 3487, 3808, 4411
MhlI GDGCHC 4 cut(s) 113, 1537, 2082, 4431
MlsI TGGCCA 1 cut(s) 1781
MluI ACGCGT 1 cut(s) 643
MluNI TGGCCA 1 cut(s) 1781
MlyI GAGTC 8 cut(s) 251, 266, 947, 1574, 1760, 1834, 3549, 3871
MmeI TCCRAC 5 cut(s) 57, 396, 1918, 2507, 2813
Mox20I TGGCCA 1 cut(s) 1781
Mph1103I ATGCAT 2 cut(s) 1308, 1948
MscI TGGCCA 1 cut(s) 1781
MslI CAYNNNNRTG 3 cut(s) 20, 1311, 1958
Msp20I TGGCCA 1 cut(s) 1781
MspA1I CMGCKG 3 cut(s) 2246, 3464, 3938
MspI CCGG 3 cut(s) 194, 877, 2809
MspR9I CCNGG 7 cut(s) 55, 202, 1634, 2810, 3702, 3747, 4315
MunI CAATTG 2 cut(s) 1010, 2859
Mva1269I GAATGC 2 cut(s) 1829, 1932
MvaI CCWGG 6 cut(s) 55, 202, 1634, 3702, 3747, 4315
MvnI CGCG 2 cut(s) 237, 645
NciI CCSGG 1 cut(s) 2810
NcoI CCATGG 3 cut(s) 2910, 3292, 3726
NdeI CATATG 2 cut(s) 2275, 3841
NmeAIII GCCGAG 1 cut(s) 4313
NmuCI GTSAC 7 cut(s) 43, 363, 980, 1262, 1768, 1837, 1951
NsiI ATGCAT 2 cut(s) 1308, 1948
NspI RCATGY 4 cut(s) 1310, 1901, 2094, 3989
NspV TTCGAA 2 cut(s) 786, 2345
OliI CACNNNNGTG 1 cut(s) 20
PaeI GCATGC 2 cut(s) 1310, 3989
PaeR7I CTCGAG 1 cut(s) 1088
PceI AGGCCT 2 cut(s) 1805, 3084
PcsI WCGNNNNNNNCGW 3 cut(s) 642, 1486, 3413
PctI GAATGC 2 cut(s) 1829, 1932
PflMI CCANNNNNTGG 1 cut(s) 833
PleI GAGTC 8 cut(s) 250, 265, 947, 1574, 1759, 1834, 3549, 3870
PpsI GAGTC 8 cut(s) 250, 265, 947, 1574, 1759, 1834, 3549, 3870
Ppu21I YACGTR 2 cut(s) 43, 1481
PpuMI RGGWCCY 4 cut(s) 167, 449, 1814, 3578
PshAI GACNNNNGTC 1 cut(s) 3294
PshBI ATTAAT 3 cut(s) 2331, 2774, 3605
PsiI TTATAA 1 cut(s) 2535
Psp124BI GAGCTC 1 cut(s) 113
Psp5II RGGWCCY 4 cut(s) 167, 449, 1814, 3578
Psp6I CCWGG 6 cut(s) 53, 200, 1632, 3700, 3745, 4313
PspFI CCCAGC 1 cut(s) 1809
PspGI CCWGG 6 cut(s) 53, 200, 1632, 3700, 3745, 4313
PspPPI RGGWCCY 4 cut(s) 167, 449, 1814, 3578
PstI CTGCAG 5 cut(s) 1122, 1993, 3592, 3697, 4020
PstNI CAGNNNCTG 5 cut(s) 946, 1079, 1388, 1754, 3701
PsuI RGATCY 9 cut(s) 711, 721, 1510, 2162, 2488, 3257, 3487, 3808, 4411
PvuII CAGCTG 3 cut(s) 2246, 3464, 3938
RseI CAYNNNNRTG 3 cut(s) 20, 1311, 1958
SacI GAGCTC 1 cut(s) 113
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 8 cut(s) 251, 266, 947, 1574, 1760, 1834, 3549, 3871
ScrFI CCNGG 7 cut(s) 55, 202, 1634, 2810, 3702, 3747, 4315
SduI GDGCHC 4 cut(s) 113, 1537, 2082, 4431
SfcI CTRYAG 6 cut(s) 1118, 1989, 2757, 3588, 3693, 4016
Sfr274I CTCGAG 1 cut(s) 1088
SfuI TTCGAA 2 cut(s) 786, 2345
SlaI CTCGAG 1 cut(s) 1088
SmiMI CAYNNNNRTG 3 cut(s) 20, 1311, 1958
SmlI CTYRAG 4 cut(s) 650, 1088, 3718, 3812
SmoI CTYRAG 4 cut(s) 650, 1088, 3718, 3812
SnaBI TACGTA 1 cut(s) 1481
SpeI ACTAGT 2 cut(s) 1376, 2285
SphI GCATGC 2 cut(s) 1310, 3989
SseBI AGGCCT 2 cut(s) 1805, 3084
SspI AATATT 2 cut(s) 408, 2634
SstI GAGCTC 1 cut(s) 113
StuI AGGCCT 2 cut(s) 1805, 3084
StyD4I CCNGG 7 cut(s) 53, 200, 1632, 2808, 3700, 3745, 4313
StyI CCWWGG 7 cut(s) 1155, 1464, 2910, 3261, 3292, 3726, 4149
TaaI ACNGT 9 cut(s) 524, 691, 761, 1137, 2121, 2420, 2583, 3104, 3154
TaiI ACGT 6 cut(s) 45, 1099, 1483, 3430, 4386, 4455
TaqI TCGA 8 cut(s) 350, 786, 1089, 1099, 2345, 2566, 3416, 3774
TaqII GACCGA 2 cut(s) 52, 951
TatI WGTACW 6 cut(s) 372, 1054, 2578, 3440, 3909, 4217
TauI GCSGC 4 cut(s) 191, 227, 1829, 3473
TseFI GTSAC 7 cut(s) 43, 363, 980, 1262, 1768, 1837, 1951
Tsp45I GTSAC 7 cut(s) 43, 363, 980, 1262, 1768, 1837, 1951
TspGWI ACGGA 1 cut(s) 1442
Van91I CCANNNNNTGG 1 cut(s) 833
VneI GTGCAC 1 cut(s) 1533
VspI ATTAAT 3 cut(s) 2331, 2774, 3605
XagI CCTNNNNNAGG 1 cut(s) 52
XceI RCATGY 4 cut(s) 1310, 1901, 2094, 3989
XcmI CCANNNNNNNNNTGG 1 cut(s) 4266
XhoI CTCGAG 1 cut(s) 1088
ZrmI AGTACT 1 cut(s) 374
Zsp2I ATGCAT 2 cut(s) 1308, 1948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.