Rmu_sc0027531.1_g000001

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027531.1
OG0002933
Physical Location & Seq
Reverse (-)
1 .. 6780
6780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027531.1_g000001.1.cds

Sequence Viewer

Length: 2571 bp
atgtcggcttctcaaaccctaggcggggtttcacgcgccggccgcctcctcggcccctccctcgacaaaatcatcaagaacgccgcctggcgcaagcactcccatttggtctccgcctgcaaatccgccctcgacaagctcgactccctctccgactcccccctcaacgaccccaactcccccatcgtcggcttcctcttccccgacgccgagtccgtcctcgcccccctcctcctcgccgtcgactccgcccacgccaaggtcgtcgagcccgccctcgaatgcgccttcaagctcttctccctcggtctcttccgcggccagatcgacgccgccgcccccaagttcgtcctcttcaagctcatcgactccgtctgcaagtgcggcggcctcggcgacgaggcgatcgagctcgccgtcctcaaggctctgctcgccgccgttagatcgccgttagtggcgatcggaggggactgcttggtgagtattgtgaggacttgttacaatgtgtaccttggtggattgaatgggaccaatcagatctgtgccaagtcggttttggctcagattatggtcatcgtcttcaccagagtggaggccgatgctatgtacgtcaccatctccagggtttcggtcagcgaattgctggaatttactgataagaatctgaatgaagggagctccattatgttctgccagaatttcatcaatgaggttatggaagctagttatgggggtcctgatagtatcaaaatggcggctcccgcccccaggagattgcaaaatggaaatgccgccgctggacgcggtgagtcgggggctggagagtccaatgatgaagccgaatctggcgagggaggtggtagtagtaagattcgggatgatggatttcttttgtttaagaatttgtgtaagttgtcgatgaagttctcgtcacaggagtactctgatgatcaaatactattgaggggtaaaatattgtcgttggagcttctgaaggtggttatggacaatgggggtcctatttggctctctaatgacaggtttctcaatggcatcaagcagttcctatgcttatcattgctaaaaaacagtgcgctgtcagttatggctattttccagctccagtgttctattttcacgagcttgttatcaaaatttagatcagggttgaaagcagaaatcggtatattttttcccatgctcgtcttacgcgtgcttgagaatgttctccagcctagtttcttgcagaaaatgacggtcctcaatttattagagaagatctctcaggatccacagattatcattgacatttttgtcaactatgactgtgatgtggattctccaaacattttcgaaaggattgtcaacggccttctgaaaactgctctaggtccaccttctggttcaacaacaacattatctccagttcaggatattactttccggcatgaatcagtgaagtgcttggttaacatcattaagtcaatgggagcttggatggaccgacagagactgggagactcctatctgccaaataccaatgagagtgacactacatcagagaaaacagagaatcaattgaccctaaacggtgaagaaggaaccactttagacaatgaagtaaatccagaaggaagttcagatgctgcaactctcgagcaacgtcgagcttttaaacttgaactgcagaaaggtatctcactgttcaataggaaaccttccaagggcattgagtttctgataagcacaaaaaaaattgggggttctccagcagatgtggcttctttcctgaagaacaacactgcgggcctaaatgaaaccatgattggtgactatttgggtgaaagggaagaattttctctaaaggttatgcatgcttatgtagattcctttaatttcaaagggatagattttggtgaagcaattaggtttttcctgcgggggttcaagttacctggagaagcacagaaaattgaccgaatcatggagaagtttgctgagcgttattgtaaatgcagtccgaattcatttactagtgcagatactgcttatgtccttgcatactctgtgataatgctcaacacagatgcccataatatcatggttaaagataagatgaccaaggctgatttcatacgtaacaaccgaggaatagatgatggcaaggatctacctgaggaatatcttggtgcactatatgatcagattcagactgaagaaaaagcgttgggtgcaaatggtcttcttataaaggacatccaagagcaatttaaggcgaagtcagggaaatcagagtctgtttatcattctgtgacagatgtggccatattgaggttcatggtggaggcctgctggggtcctatgctggccgcattcagtgtgactctggaccaaagtgatgacaggcttgctactgctcaatgcttactgggtttccgacatgcaattcatgttactgctctgatgggaatgcagacccagagagatgcatttgtcacatcaatggcaaagtttacttatctacataatgctgcagatatgaggcaaaagaatgttgacgctgtgaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

857

Amino Acids

93.78

Weight (kDa)

5.64

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2252
AasI GACNNNNNNGTC 1 cut(s) 1316
AccB7I CCANNNNNTGG 1 cut(s) 1403
AccI GTMKAC 1 cut(s) 243
AccII CGCG 4 cut(s) 36, 318, 807, 1215
AclWI GGATC 3 cut(s) 1286, 1299, 2178
AcoI YGGCCR 4 cut(s) 40, 319, 2325, 2370
AcsI RAATTY 6 cut(s) 650, 700, 904, 1157, 1856, 2026
AcuI CTGAAG 3 cut(s) 1016, 1814, 2238
AcyI GRCGYC 2 cut(s) 207, 330
AfaI GTAC 3 cut(s) 512, 611, 944
AflIII ACRYGT 1 cut(s) 1213
AgsI TTSAA 9 cut(s) 292, 358, 526, 1174, 1410, 1685, 1711, 1903, 1951
AhdI GACNNNNNGTC 1 cut(s) 211
AhlI ACTAGT 1 cut(s) 2036
AjnI CCWGG 4 cut(s) 86, 623, 770, 1957
AjuI GAANNNNNNNTTGG 4 cut(s) 1812, 1844, 2117, 2149
AleI CACNNNNGTG 1 cut(s) 590
Alw21I GWGCWC 3 cut(s) 414, 683, 2197
Alw26I GTCTC 4 cut(s) 115, 314, 1507, 1515
Alw44I GTGCAC 1 cut(s) 2193
AlwI GGATC 3 cut(s) 1286, 1299, 2178
AlwNI CAGNNNCTG 4 cut(s) 1516, 1649, 2048, 2300
Ama87I CYCGRG 1 cut(s) 1658
ApaLI GTGCAC 1 cut(s) 2193
ApeKI GCWGC 2 cut(s) 1649, 2534
ApoI RAATTY 6 cut(s) 650, 700, 904, 1157, 1856, 2026
ArsI GACNNNNNNTTYG 2 cut(s) 1350, 1382
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 4 cut(s) 38, 93, 287, 1099
AsuHPI GGTGA 8 cut(s) 493, 577, 607, 821, 1607, 1844, 1856, 1931
AsuII TTCGAA 1 cut(s) 1356
AvaI CYCGRG 1 cut(s) 1658
AvaII GGWCC 8 cut(s) 531, 737, 1019, 1261, 1394, 1504, 2360, 2392
AvrII CCTAGG 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 2178
BaeGI GKGCMC 1 cut(s) 2197
BalI TGGCCA 1 cut(s) 2327
BamHI GGATCC 1 cut(s) 1291
BanII GRGCYC 3 cut(s) 273, 414, 683
BauI CACGAG 1 cut(s) 1141
BbsI GAAGAC 2 cut(s) 574, 2237
Bbv12I GWGCWC 3 cut(s) 414, 683, 2197
BbvI GCAGC 2 cut(s) 1636, 2521
BccI CCATC 6 cut(s) 191, 626, 878, 1495, 2156, 2461
BceAI ACGGC 5 cut(s) 224, 401, 425, 436, 1387
BciT130I CCWGG 4 cut(s) 88, 625, 772, 1959
BclI TGATCA 2 cut(s) 952, 2203
BcoDI GTCTC 4 cut(s) 115, 314, 1507, 1515
BcuI ACTAGT 1 cut(s) 2036
BfaI CTAG 5 cut(s) 20, 726, 1239, 1391, 2037
BfmI CTRYAG 2 cut(s) 1688, 2535
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 2 cut(s) 540, 1281
BlnI CCTAGG 1 cut(s) 19
BlpI GCTNAGC 1 cut(s) 2001
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 4 cut(s) 88, 625, 772, 1959
Bme18I GGWCC 8 cut(s) 531, 737, 1019, 1261, 1394, 1504, 2360, 2392
BmeRI GACNNNNNGTC 1 cut(s) 211
BmeT110I CYCGRG 1 cut(s) 1658
BmiI GGNNCC 8 cut(s) 55, 532, 738, 762, 1020, 1293, 1606, 2361
BmrFI CCNGG 4 cut(s) 88, 625, 772, 1959
BmrI ACTGGG 2 cut(s) 1526, 2441
BmsI GCATC 5 cut(s) 592, 1065, 1636, 2080, 2479
BmuI ACTGGG 2 cut(s) 1526, 2441
BpiI GAAGAC 2 cut(s) 574, 2237
BplI GAGNNNNNCTC 2 cut(s) 1268, 1300
BpmI CTGGAG 7 cut(s) 607, 843, 1109, 1217, 1410, 1755, 1980
Bpu1102I GCTNAGC 1 cut(s) 2001
Bpu14I TTCGAA 1 cut(s) 1356
BpuEI CTTGAG 2 cut(s) 407, 1241
BsaAI YACGTR 1 cut(s) 2141
BsaBI GATNNNNATC 1 cut(s) 663
BsaHI GRCGYC 2 cut(s) 207, 330
BsaI GGTCTC 2 cut(s) 115, 314
BsaXI ACNNNNNCTCC 6 cut(s) 134, 161, 164, 191, 1408, 1438
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 4 cut(s) 1126, 1427, 1521, 2436
Bse21I CCTNAGG 1 cut(s) 2178
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 4 cut(s) 88, 625, 772, 1959
BseGI GGATG 3 cut(s) 886, 1506, 2259
BseJI GATNNNNATC 1 cut(s) 663
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 4 cut(s) 578, 1301, 1992, 2169
BseNI ACTGG 4 cut(s) 1126, 1427, 1521, 2436
BseRI GAGGAG 3 cut(s) 38, 221, 224
BseSI GKGCMC 1 cut(s) 2197
BseX3I CGGCCG 1 cut(s) 40
BseXI GCAGC 2 cut(s) 1636, 2521
BseYI CCCAGC 1 cut(s) 2355
BsgI GTGCAG 1 cut(s) 2061
Bsh1236I CGCG 4 cut(s) 36, 318, 807, 1215
Bsh1285I CGRYCG 3 cut(s) 43, 408, 465
BsiEI CGRYCG 3 cut(s) 43, 408, 465
BsiHKAI GWGCWC 3 cut(s) 414, 683, 2197
BsiHKCI CYCGRG 1 cut(s) 1658
BsiSI CCGG 2 cut(s) 39, 1447
BslFI GGGAC 2 cut(s) 485, 544
BsmAI GTCTC 4 cut(s) 115, 314, 1507, 1515
BsmFI GGGAC 2 cut(s) 485, 544
BsmI GAATGC 3 cut(s) 287, 2375, 2478
Bso31I GGTCTC 2 cut(s) 115, 314
BsoBI CYCGRG 1 cut(s) 1658
Bsp119I TTCGAA 1 cut(s) 1356
Bsp1286I GDGCHC 4 cut(s) 273, 414, 683, 2197
Bsp1720I GCTNAGC 1 cut(s) 2001
BspCNI CTCAG 4 cut(s) 577, 1300, 1993, 2170
BspFNI CGCG 4 cut(s) 36, 318, 807, 1215
BspLI GGNNCC 8 cut(s) 55, 532, 738, 762, 1020, 1293, 1606, 2361
BspMAI CTGCAG 2 cut(s) 1692, 2539
BspPI GGATC 3 cut(s) 1286, 1299, 2178
BspQI GCTCTTC 1 cut(s) 302
BspT104I TTCGAA 1 cut(s) 1356
BspTNI GGTCTC 2 cut(s) 115, 314
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 4 cut(s) 1126, 1427, 1521, 2436
BssAI RCCGGY 1 cut(s) 38
BssNI GRCGYC 2 cut(s) 207, 330
BssSI CACGAG 1 cut(s) 1141
BssT1I CCWWGG 5 cut(s) 19, 258, 514, 1725, 2124
Bst2BI CACGAG 1 cut(s) 1141
Bst2UI CCWGG 4 cut(s) 88, 625, 772, 1959
Bst4CI ACNGT 5 cut(s) 1094, 1261, 1331, 1595, 1707
Bst6I CTCTTC 4 cut(s) 203, 302, 317, 359
BstACI GRCGYC 2 cut(s) 207, 330
BstAPI GCANNNNNTGC 1 cut(s) 2048
BstBAI YACGTR 1 cut(s) 2141
BstBI TTCGAA 1 cut(s) 1356
BstDEI CTNAG 4 cut(s) 564, 1287, 2001, 2178
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 3 cut(s) 886, 1506, 2259
BstFNI CGCG 4 cut(s) 36, 318, 807, 1215
BstHHI GCGC 4 cut(s) 38, 93, 287, 1099
BstMAI GTCTC 4 cut(s) 115, 314, 1507, 1515
BstMCI CGRYCG 3 cut(s) 43, 408, 465
BstMWI GCNNNNNNNGC 9 cut(s) 42, 51, 384, 393, 434, 764, 1781, 2048, 2234
BstNI CCWGG 4 cut(s) 88, 625, 772, 1959
BstNSI RCATGY 2 cut(s) 1880, 2447
BstSCI CCNGG 4 cut(s) 86, 623, 770, 1957
BstSFI CTRYAG 2 cut(s) 1688, 2535
BstSLI GKGCMC 1 cut(s) 2197
BstSNI TACGTA 1 cut(s) 2141
BstUI CGCG 4 cut(s) 36, 318, 807, 1215
BstV1I GCAGC 2 cut(s) 1636, 2521
BstV2I GAAGAC 2 cut(s) 574, 2237
BstX2I RGATCY 4 cut(s) 540, 1281, 1291, 2170
BstYI RGATCY 4 cut(s) 540, 1281, 1291, 2170
BstZI CGGCCG 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 2178
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 3 cut(s) 886, 1506, 2259
BtsI GCAGTG 1 cut(s) 1803
BtsIMutI CAGTG 6 cut(s) 1099, 1133, 1464, 1703, 1803, 2386
CaiI CAGNNNCTG 4 cut(s) 1516, 1649, 2048, 2300
CfoI GCGC 4 cut(s) 38, 93, 287, 1099
Cfr10I RCCGGY 1 cut(s) 38
Cfr42I CCGCGG 1 cut(s) 319
CseI GACGC 3 cut(s) 215, 338, 813
Csp6I GTAC 3 cut(s) 511, 610, 943
CviAII CATG 9 cut(s) 1201, 1451, 1825, 1877, 1987, 2104, 2341, 2444, 2453
CviQI GTAC 3 cut(s) 511, 610, 943
DdeI CTNAG 4 cut(s) 564, 1287, 2001, 2178
DraI TTTAAA 1 cut(s) 1678
DrdI GACNNNNNNGTC 1 cut(s) 1316
DriI GACNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 4 cut(s) 40, 319, 2325, 2370
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 4 cut(s) 203, 302, 317, 359
Eam1105I GACNNNNNGTC 1 cut(s) 211
EarI CTCTTC 4 cut(s) 203, 302, 317, 359
EciI GGCGGA 3 cut(s) 103, 115, 238
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco105I TACGTA 1 cut(s) 2141
Eco130I CCWWGG 5 cut(s) 19, 258, 514, 1725, 2124
Eco147I AGGCCT 1 cut(s) 2351
Eco24I GRGCYC 3 cut(s) 273, 414, 683
Eco31I GGTCTC 2 cut(s) 115, 314
Eco47I GGWCC 8 cut(s) 531, 737, 1019, 1261, 1394, 1504, 2360, 2392
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
Eco57I CTGAAG 3 cut(s) 1016, 1814, 2238
Eco81I CCTNAGG 1 cut(s) 2178
Eco88I CYCGRG 1 cut(s) 1658
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 3 cut(s) 737, 1019, 2360
EcoRI GAATTC 1 cut(s) 2026
EcoRII CCWGG 4 cut(s) 86, 623, 770, 1957
EcoT14I CCWWGG 5 cut(s) 19, 258, 514, 1725, 2124
EcoT22I ATGCAT 2 cut(s) 1878, 2494
EcoT38I GRGCYC 3 cut(s) 273, 414, 683
ErhI CCWWGG 5 cut(s) 19, 258, 514, 1725, 2124
FaeI CATG 9 cut(s) 1204, 1454, 1828, 1880, 1990, 2107, 2344, 2447, 2456
FaqI GGGAC 2 cut(s) 485, 544
FatI CATG 9 cut(s) 1200, 1450, 1824, 1876, 1986, 2103, 2340, 2443, 2452
FauI CCCGC 5 cut(s) 17, 280, 772, 1801, 1935
FbaI TGATCA 2 cut(s) 952, 2203
FblI GTMKAC 1 cut(s) 243
FokI GGATG 3 cut(s) 893, 1513, 2246
FriOI GRGCYC 3 cut(s) 273, 414, 683
FspBI CTAG 5 cut(s) 20, 726, 1239, 1391, 2037
GlaI GCGC 4 cut(s) 37, 92, 286, 1098
GsaI CCCAGC 1 cut(s) 2359
GsuI CTGGAG 7 cut(s) 607, 843, 1109, 1217, 1410, 1755, 1980
HapII CCGG 2 cut(s) 39, 1447
HgaI GACGC 3 cut(s) 215, 338, 813
HhaI GCGC 4 cut(s) 38, 93, 287, 1099
Hin1I GRCGYC 2 cut(s) 207, 330
Hin1II CATG 9 cut(s) 1204, 1454, 1828, 1880, 1990, 2107, 2344, 2447, 2456
Hin6I GCGC 4 cut(s) 36, 91, 285, 1097
HinP1I GCGC 4 cut(s) 36, 91, 285, 1097
HincII GTYRAC 5 cut(s) 244, 1321, 1369, 1474, 2560
HindII GTYRAC 5 cut(s) 244, 1321, 1369, 1474, 2560
HpaI GTTAAC 1 cut(s) 1474
HpaII CCGG 2 cut(s) 39, 1447
HphI GGTGA 8 cut(s) 493, 577, 607, 821, 1607, 1844, 1856, 1931
Hpy166II GTNNAC 9 cut(s) 244, 511, 1321, 1369, 1397, 1474, 2195, 2517, 2560
Hpy8I GTNNAC 9 cut(s) 244, 511, 1321, 1369, 1397, 1474, 2195, 2517, 2560
Hpy99I CGWCG 7 cut(s) 191, 209, 245, 269, 332, 401, 1671
HpyAV CCTTC 8 cut(s) 298, 668, 991, 1385, 1410, 1595, 1628, 1731
HpyCH4III ACNGT 5 cut(s) 1094, 1261, 1331, 1595, 1707
HpyCH4IV ACGT 3 cut(s) 612, 1666, 2140
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 51, 384, 393, 434, 764, 1781, 2048, 2234
HpyF3I CTNAG 4 cut(s) 564, 1287, 2001, 2178
HpySE526I ACGT 3 cut(s) 612, 1666, 2140
Hsp92I GRCGYC 2 cut(s) 207, 330
Hsp92II CATG 9 cut(s) 1204, 1454, 1828, 1880, 1990, 2107, 2344, 2447, 2456
HspAI GCGC 4 cut(s) 36, 91, 285, 1097
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 2 cut(s) 952, 2203
KspAI GTTAAC 1 cut(s) 1474
KspI CCGCGG 1 cut(s) 319
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 6 cut(s) 678, 686, 766, 988, 1128, 1493
Lsp1109I GCAGC 2 cut(s) 1636, 2521
LweI GCATC 5 cut(s) 592, 1065, 1636, 2080, 2479
MaeI CTAG 5 cut(s) 20, 726, 1239, 1391, 2037
MaeII ACGT 3 cut(s) 612, 1666, 2140
MfeI CAATTG 1 cut(s) 1580
MflI RGATCY 4 cut(s) 540, 1281, 1291, 2170
MhlI GDGCHC 4 cut(s) 273, 414, 683, 2197
MlsI TGGCCA 1 cut(s) 2327
MluI ACGCGT 1 cut(s) 1213
MluNI TGGCCA 1 cut(s) 2327
MmeI TCCRAC 3 cut(s) 177, 966, 2464
Mox20I TGGCCA 1 cut(s) 2327
Mph1103I ATGCAT 2 cut(s) 1878, 2494
MroNI GCCGGC 1 cut(s) 38
MscI TGGCCA 1 cut(s) 2327
MseI TTAA 7 cut(s) 900, 1473, 1482, 1677, 1896, 2109, 2274
MslI CAYNNNNRTG 3 cut(s) 590, 1881, 2504
Msp20I TGGCCA 1 cut(s) 2327
MspA1I CMGCKG 2 cut(s) 318, 800
MspI CCGG 2 cut(s) 39, 1447
MspR9I CCNGG 4 cut(s) 88, 625, 772, 1959
MunI CAATTG 1 cut(s) 1580
Mva1269I GAATGC 3 cut(s) 287, 2375, 2478
MvaI CCWGG 4 cut(s) 88, 625, 772, 1959
MvnI CGCG 4 cut(s) 36, 318, 807, 1215
MwoI GCNNNNNNNGC 9 cut(s) 42, 51, 384, 393, 434, 764, 1781, 2048, 2234
NaeI GCCGGC 1 cut(s) 40
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 9 cut(s) 1204, 1454, 1828, 1880, 1990, 2107, 2344, 2447, 2456
NlaIV GGNNCC 8 cut(s) 55, 532, 738, 762, 1020, 1293, 1606, 2361
NmeAIII GCCGAG 3 cut(s) 30, 235, 372
NmuCI GTSAC 7 cut(s) 613, 933, 1550, 1832, 2314, 2383, 2497
NsiI ATGCAT 2 cut(s) 1878, 2494
NspI RCATGY 2 cut(s) 1880, 2447
NspV TTCGAA 1 cut(s) 1356
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 1880
PaeR7I CTCGAG 1 cut(s) 1658
PceI AGGCCT 1 cut(s) 2351
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 7 cut(s) 138, 213, 261, 405, 414, 1212, 2146
PctI GAATGC 3 cut(s) 287, 2375, 2478
PdiI GCCGGC 1 cut(s) 40
PfeI GAWTC 9 cut(s) 664, 845, 874, 1340, 1454, 1576, 1889, 1983, 2209
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 1403
Ple19I CGATCG 2 cut(s) 408, 465
Ppu21I YACGTR 1 cut(s) 2141
PpuMI RGGWCCY 3 cut(s) 737, 1019, 2360
PsiI TTATAA 1 cut(s) 2252
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp5II RGGWCCY 3 cut(s) 737, 1019, 2360
Psp6I CCWGG 4 cut(s) 86, 623, 770, 1957
PspFI CCCAGC 1 cut(s) 2355
PspGI CCWGG 4 cut(s) 86, 623, 770, 1957
PspN4I GGNNCC 8 cut(s) 55, 532, 738, 762, 1020, 1293, 1606, 2361
PspPPI RGGWCCY 3 cut(s) 737, 1019, 2360
PstI CTGCAG 2 cut(s) 1692, 2539
PstNI CAGNNNCTG 4 cut(s) 1516, 1649, 2048, 2300
PsuI RGATCY 4 cut(s) 540, 1281, 1291, 2170
PsyI GACNNNGTC 1 cut(s) 371
PvuI CGATCG 2 cut(s) 408, 465
RsaI GTAC 3 cut(s) 512, 611, 944
RsaNI GTAC 3 cut(s) 511, 610, 943
RseI CAYNNNNRTG 3 cut(s) 590, 1881, 2504
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 1 cut(s) 319
SalI GTCGAC 1 cut(s) 242
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 7 cut(s) 900, 1473, 1482, 1677, 1896, 2109, 2274
ScaI AGTACT 1 cut(s) 944
ScrFI CCNGG 4 cut(s) 88, 625, 772, 1959
SduI GDGCHC 4 cut(s) 273, 414, 683, 2197
SfaNI GCATC 5 cut(s) 592, 1065, 1636, 2080, 2479
SfcI CTRYAG 2 cut(s) 1688, 2535
Sfr274I CTCGAG 1 cut(s) 1658
Sfr303I CCGCGG 1 cut(s) 319
SfuI TTCGAA 1 cut(s) 1356
SgrBI CCGCGG 1 cut(s) 319
SinI GGWCC 8 cut(s) 531, 737, 1019, 1261, 1394, 1504, 2360, 2392
SlaI CTCGAG 1 cut(s) 1658
SmiMI CAYNNNNRTG 3 cut(s) 590, 1881, 2504
SmlI CTYRAG 3 cut(s) 422, 1220, 1658
SmoI CTYRAG 3 cut(s) 422, 1220, 1658
SnaBI TACGTA 1 cut(s) 2141
SpeI ACTAGT 1 cut(s) 2036
SphI GCATGC 1 cut(s) 1880
SseBI AGGCCT 1 cut(s) 2351
SspI AATATT 1 cut(s) 978
SspMI CTAG 5 cut(s) 20, 726, 1239, 1391, 2037
SstI GAGCTC 2 cut(s) 414, 683
StuI AGGCCT 1 cut(s) 2351
StyD4I CCNGG 4 cut(s) 86, 623, 770, 1957
StyI CCWWGG 5 cut(s) 19, 258, 514, 1725, 2124
TaaI ACNGT 5 cut(s) 1094, 1261, 1331, 1595, 1707
TaiI ACGT 3 cut(s) 615, 1669, 2143
TaqII GACCGA 4 cut(s) 296, 622, 1521, 1995
TatI WGTACW 1 cut(s) 942
TfiI GAWTC 9 cut(s) 664, 845, 874, 1340, 1454, 1576, 1889, 1983, 2209
Tru1I TTAA 7 cut(s) 900, 1473, 1482, 1677, 1896, 2109, 2274
Tru9I TTAA 7 cut(s) 900, 1473, 1482, 1677, 1896, 2109, 2274
TscAI CASTG 6 cut(s) 1099, 1133, 1464, 1710, 1810, 2386
TseFI GTSAC 7 cut(s) 613, 933, 1550, 1832, 2314, 2383, 2497
TseI GCWGC 2 cut(s) 1649, 2534
Tsp45I GTSAC 7 cut(s) 613, 933, 1550, 1832, 2314, 2383, 2497
TspGWI ACGGA 2 cut(s) 205, 361
TspRI CASTG 6 cut(s) 1099, 1133, 1464, 1710, 1810, 2386
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 1403
VneI GTGCAC 1 cut(s) 2193
VpaK11BI GGWCC 8 cut(s) 531, 737, 1019, 1261, 1394, 1504, 2360, 2392
XapI RAATTY 6 cut(s) 650, 700, 904, 1157, 1856, 2026
XceI RCATGY 2 cut(s) 1880, 2447
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XhoI CTCGAG 1 cut(s) 1658
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 5 cut(s) 20, 726, 1239, 1391, 2037
ZrmI AGTACT 1 cut(s) 944
Zsp2I ATGCAT 2 cut(s) 1878, 2494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.