Rmu_sc0000847.1_g000049

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000847.1
OG0002933
Physical Location & Seq
Reverse (-)
209162 .. 219736
10575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000847.1_g000049.1.cds

Sequence Viewer

Length: 5073 bp
atgtcggcttctcaaaccctaggcggggtttcacgcgccggccgcctcctcggcccctccctcgacaaaatcatcaagaacgccgcctggcgcaagcactcccatttggtctccgcctgcaaatccgccctcgacaagctcgactccctctccgactcccccctcaacgaccccaactcccccatcgtcggcttcctcttccccgacgccgagtccgtcctcgcccccctcctcctcgccgtcgactccgcccacgccaaggtcgtcgagcccgccctcgaatgcgccttcaagctcttctccctcggtctcttccgcggccagatcgacgccgccgcccccaagttcgtcctcttcaagctcatcgactccgtctgcaagtgcggcggcctcggcgacgaggcgatcgagctcgccgtcctcaaggctctgctcgccgccgttagatcgccgttagtggcgatcggaggggactgcttggtgagtattgtgaggacttgttacaatgtgtaccttggtggattgaatgggaccaatcagatctgtgccaagtcggttttggctcagattatggtcatcgtcttcaccagagtggaggccgatgctatgtacgtcaccatctccagggtttcggtcagcgaattgctggaatttactgataagaatctgaatgaagggagctccattatgttctgccagaatttcatcaatgaggttatggaagctagttatgggggtcctgatagtatcaaaatggcggctcccgcccccaggagattgcaaaatggaaatgccgccgctggacgcggtgagtcgggggctggagagtccaatgatgaagccgaatctggcgagggaggtggtagtagtaagattcgggatgatggatttcttttgtttaagaatttgtgtaagttgtcgatgaagttctcgtcacaggagtactctgatgatcaaatactattgaggggtaaaatattgtcgttggagcttctgaaggtggttatggacaatgggggtcctatttggctctctaatgacaggtttctcaatggcatcaagcagttcctatgcttatcattgctaaaaaacagtgcgctgtcagttatggctattttccagctccagtgttctattttcacgagcttgttatcaaaatttagatcagggttgaaagcagaaatcggtatattttttcccatgctcgtcttacgcgtgcttgagaatgttctccagcctagtttcttgcagaaaatgacggtcctcaatttattagagaagatctctcaggatccacagattatcattgacatttttgtcaactatgactgtgatgtggattctccaaacattttcgaaaggattgtcaacggccttctgaaaactgctctaggtccaccttctggttcaacaacaacattatctccagttcaggatattactttccggcatgaatcagtgaagtgcttggttaacatcattaagtcaatgggagcttggatggaccgacagagactgggagactcctatctgccaaataccaatgagagtgacactacatcagagaaaacagagaatcaattgaccctaaacggtgaagaaggaaccactttagacaatgaagtacatccagaaggaagttcagatgctgcaactctcgagcaacgtagagcttttaaacttgaactgcagaaaggtatctcactgttcaataggaaaccttccaagggcattgagtttctgataagcacaaaaaaaattgggggttctccagcagatgtggcttctttcctgaagaacaacactgcgggcctaaatgaaaccatgattggtgactatttgggtgaaagggaagaattttctctaaaggttatgcatgcttatgtagattcctttaatttcaaagggatagattttggtgaagcaattaggtttttcctgcgggggttcaagttacctggagaagcacagaaaattgaccgaatcatggagaagtttgctgagcgttattgtaaatgcagtccgaattcatttactagtgcagatactgcttatgtccttgcatactccgtgataatgctcaacacggatgcccataatatcatggttaaagataagattgttaaaaatgaaattaagatgaaggctgactcttctgtaccacagagcaagcaggaaaacagctttaacaaattattgggcctggatggtatcctgaatctagtaactgggaagcagactgaagaaaaagcgttgggtgcaaatggtcttcttataaaggacatccaagagcaatttaaggcgaagtcagggaaatcagagtctgtttatcattctgtgacagatgtggccatattgaggttcatggtggaagcctgctggggtcctatgctggccgcattcagtgtgactctggaccaaagtgatgacaggcttgctactgctcaatgcttactgggtttccgacatgcaattcatgttactgctctgatgggaatgcagacccagagagatgcatttgtcacatcaatggcaaagtttacttatctacataatgctgcagatatgaggcaaaagaatgttgacgctgtgaaggcaataatagcaatcgccgttgaagatggtaatcatcttcaggaagcatgggagcacatattaacatgcctttcccgaattgagcatctgcaactgttgggtgagggcgcacctactgatgcaacctttttcagtggatctaatgttgaagcggatgacaaatcaccaaagcctattggttttgcttctctaaagaaaaaaggaactatccagaatccagctgtgatggctgttgttcgtgggggttcatatgatagcactagtattggaatcaacacttcgggactggtatctccagaacagattaataattttatttcgaatttgaatttgctggatcagattgggaattttgagttgaaccatgtatttgctcatagccaaagcttgaacagtgaagccatagttgcttttgtgaagtccttatgtaaagtttccatgtcagaattgcagtctccaacagatcctcgtgttttcagcctcacaaaaatagttgaaatagcgcattataatatgaaccgcatcagattagtgtggtctcgaatatggaatgtactgtcagatttctttgtatcagttggcttgtcagagaatctttcagttgcaatatttgtgatggattccttgcgtcagcttgctatgaaattcttagagcgtgaggagctggcaaattacaatttccagaatgaatttttaagaccatttgtgattgttatgcagaaaagcagctctacagaaattagggaattaatagttcggtgcatttcacaaatggtcctcagccgagtcaataatgtgaaatctggctggaaaagtgtttttatggttttcacaattgctgctgctgatgagaggaaaaatatagtcttgttggcctttgagaccatggaaaagatagtcagagagtatttcccttacataactgagacagagacattgacttttactgattgtgttagatgcctcttaaccttcacaaatagcagattcaatagtgatgttagcctcaatgctattgcatttctgcgtttctgtgccgtcaaacttgcagagggaggccttgtttacaataagaacagtgagattgatggttcttcaagtcctagcgcaagtgaaggtgcttcaactgttgagaatttcaatgataaagatgatcatacatcattttgggttccattgctaacaggtttgtccaaactaacatctgatcctagatcagctatcaggaaaggatccttggaagttcttttcaacattctaaaggaccatggtcatcttttctcacacctgttttggactgcggttttcaattctgttatttttcccatatttaatggtgtcagcgataagaaagatacagacatgaaaaatgaccagtcctcgccaatttcgatgtccccacgtcctgaaggaagtacatgggattctgaaacttcagctgttgcggcggattgcctcatagatctgttcgtcagtttctttgatgttgttaggtctcaactaccaagtgtgatatccattctgacaggactcatcagaagtcccattcagggtcctgctactgcaggagttgctgcattaatgcgtttgtctagtgaggtgggtagcaggctttcagaagatgaatggagagcaatttttctggctttaaaggaggctgccacatctgcagtgcctggatttttgaaggtcttgagaaccatggataatgtcaatgttcctggtctttctgaatcttttagtgatatcgatatgtcgtctgatcaggggtactcaaatgaggatcttgaggatgataatttgcaaacagcttcatatgtggtttcaagaatcaagagtcatgtttctatgcagctactcatcttacaggtcgctgctgatttgtacaaaataaacctagaaaccttgtcagctgctaacattgcagttcttcttgaagtatattctctcgtggcatcgcacgctcaccaactgaactctgagacaattctgcagaagaagctggaaaaagtgtgttccatcctagaactcactgcaccacctattgttcattttgagaatgaatcttacaaaaactatcttaacttcctccaaaacgcacttgtggataatccatctctttccaaggaaatggacatagaagctaaactcgtggcagtctgtgaaaacatattgcagatatacctaaagtgtactgagcttcaatcttctgagcagaagcctactggtcagccagttttgcactggattcttcctttgggtacagcaaaaaaggaggaattggctaccaggactttcttagccgtctcttcattgcaggcattgagtggtttggaaaaggtttcatttaggaggcacgtttctcagttatttcctctgttggtagatcttgtgaagagtgagcacacttctggagaagttcaacatgttctaagcaacatattccagtcatgtataggcccaacaataatggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1690

Amino Acids

185.86

Weight (kDa)

5.57

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2276, 3105
AasI GACNNNNNNGTC 1 cut(s) 1316
AccB7I CCANNNNNTGG 1 cut(s) 1403
AccI GTMKAC 1 cut(s) 243
AccII CGCG 4 cut(s) 36, 318, 807, 1215
AclWI GGATC 9 cut(s) 1286, 1299, 2740, 2940, 3053, 3797, 3822, 3835, 4386
AcoI YGGCCR 4 cut(s) 40, 319, 2349, 2394
AcuI CTGAAG 6 cut(s) 1016, 1814, 2262, 2618, 4014, 4023
AcyI GRCGYC 2 cut(s) 207, 330
AflIII ACRYGT 2 cut(s) 1213, 5020
AhdI GACNNNNNGTC 1 cut(s) 211
AhlI ACTAGT 2 cut(s) 2036, 2855
AjiI CACGTC 1 cut(s) 3998
AjnI CCWGG 8 cut(s) 86, 623, 770, 1957, 2202, 4270, 4315, 4883
AjuI GAANNNNNNNTTGG 4 cut(s) 1812, 1844, 3815, 3847
AleI CACNNNNGTG 1 cut(s) 590
Alw21I GWGCWC 4 cut(s) 414, 683, 2652, 5001
AlwI GGATC 9 cut(s) 1286, 1299, 2740, 2940, 3053, 3797, 3822, 3835, 4386
AlwNI CAGNNNCTG 5 cut(s) 1516, 1649, 2048, 2324, 4271
Ama87I CYCGRG 1 cut(s) 1658
ArsI GACNNNNNNTTYG 2 cut(s) 1350, 1382
AseI ATTAAT 3 cut(s) 2901, 3344, 4175
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 7 cut(s) 38, 93, 287, 1099, 2705, 3100, 3704
AsuII TTCGAA 2 cut(s) 1356, 2915
AvaI CYCGRG 1 cut(s) 1658
AvrII CCTAGG 1 cut(s) 19
BalI TGGCCA 1 cut(s) 2351
BamHI GGATCC 2 cut(s) 1291, 3827
BanII GRGCYC 3 cut(s) 273, 414, 683
BauI CACGAG 4 cut(s) 1141, 3063, 4544, 4745
BbsI GAAGAC 2 cut(s) 574, 2261
Bbv12I GWGCWC 4 cut(s) 414, 683, 2652, 5001
BbvCI CCTCAGC 1 cut(s) 3374
BceAI ACGGC 8 cut(s) 224, 401, 425, 436, 1387, 2597, 3617, 4883
BciT130I CCWGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
BciVI GTATCC 1 cut(s) 2222
BclI TGATCA 3 cut(s) 952, 3748, 4357
BcuI ACTAGT 2 cut(s) 2036, 2855
BfmI CTRYAG 6 cut(s) 1688, 2559, 3327, 4158, 4263, 4586
BfuI GTATCC 1 cut(s) 2222
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 4 cut(s) 540, 1281, 4057, 4981
BlnI CCTAGG 1 cut(s) 19
BlpI GCTNAGC 1 cut(s) 2001
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
BmeRI GACNNNNNGTC 1 cut(s) 211
BmeT110I CYCGRG 1 cut(s) 1658
BmgBI CACGTC 1 cut(s) 3998
BmrFI CCNGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
BmrI ACTGGG 3 cut(s) 1526, 2238, 2465
BmuI ACTGGG 3 cut(s) 1526, 2238, 2465
BoxI GACNNNNGTC 1 cut(s) 3864
BpiI GAAGAC 2 cut(s) 574, 2261
BplI GAGNNNNNCTC 2 cut(s) 1268, 1300
BpmI CTGGAG 9 cut(s) 607, 843, 1109, 1217, 1410, 1755, 1980, 2874, 5028
Bpu10I CCTNAGC 1 cut(s) 3374
Bpu1102I GCTNAGC 1 cut(s) 2001
Bpu14I TTCGAA 2 cut(s) 1356, 2915
BpuEI CTTGAG 4 cut(s) 407, 1241, 4309, 4403
Bsa29I ATCGAT 1 cut(s) 4344
BsaBI GATNNNNATC 2 cut(s) 663, 2625
BsaHI GRCGYC 2 cut(s) 207, 330
BsaI GGTCTC 5 cut(s) 115, 314, 3138, 3470, 4095
BsaXI ACNNNNNCTCC 8 cut(s) 134, 161, 164, 191, 1408, 1438, 3642, 3672
Bse118I RCCGGY 1 cut(s) 38
Bse3DI GCAATG 4 cut(s) 1079, 3770, 4515, 4907
Bse8I GATNNNNATC 2 cut(s) 663, 2625
BseBI CCWGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
BseCI ATCGAT 1 cut(s) 4344
BseGI GGATG 9 cut(s) 886, 1506, 1627, 2095, 2212, 2283, 2755, 4393, 4614
BseJI GATNNNNATC 2 cut(s) 663, 2625
BseMI GCAATG 4 cut(s) 1079, 3770, 4515, 4907
BseMII CTCAG 9 cut(s) 578, 1301, 1992, 3388, 3510, 4566, 4782, 4797, 4973
BseRI GAGGAG 4 cut(s) 38, 221, 224, 3269
BseX3I CGGCCG 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 2379
BsgI GTGCAG 2 cut(s) 2061, 4614
Bsh1236I CGCG 4 cut(s) 36, 318, 807, 1215
Bsh1285I CGRYCG 3 cut(s) 43, 408, 465
BshVI ATCGAT 1 cut(s) 4344
BsiEI CGRYCG 3 cut(s) 43, 408, 465
BsiHKAI GWGCWC 4 cut(s) 414, 683, 2652, 5001
BsiHKCI CYCGRG 1 cut(s) 1658
BsiSI CCGG 2 cut(s) 39, 1447
BslFI GGGAC 5 cut(s) 485, 544, 2892, 3976, 4122
BsmBI CGTCTC 1 cut(s) 4906
BsmFI GGGAC 5 cut(s) 485, 544, 2892, 3976, 4122
BsmI GAATGC 3 cut(s) 287, 2399, 2502
Bso31I GGTCTC 5 cut(s) 115, 314, 3138, 3470, 4095
BsoBI CYCGRG 1 cut(s) 1658
Bsp119I TTCGAA 2 cut(s) 1356, 2915
Bsp1286I GDGCHC 5 cut(s) 273, 414, 683, 2652, 5001
Bsp1407I TGTACA 1 cut(s) 4479
Bsp1720I GCTNAGC 1 cut(s) 2001
Bsp19I CCATGG 3 cut(s) 3480, 3862, 4296
BspCNI CTCAG 9 cut(s) 577, 1300, 1993, 3387, 3511, 4567, 4783, 4798, 4972
BspDI ATCGAT 1 cut(s) 4344
BspFNI CGCG 4 cut(s) 36, 318, 807, 1215
BspMAI CTGCAG 5 cut(s) 1692, 2563, 4162, 4267, 4590
BspPI GGATC 9 cut(s) 1286, 1299, 2740, 2940, 3053, 3797, 3822, 3835, 4386
BspQI GCTCTTC 1 cut(s) 302
BspT104I TTCGAA 2 cut(s) 1356, 2915
BspTNI GGTCTC 5 cut(s) 115, 314, 3138, 3470, 4095
BsrDI GCAATG 4 cut(s) 1079, 3770, 4515, 4907
BsrFI RCCGGY 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 4479
BssAI RCCGGY 1 cut(s) 38
BssNI GRCGYC 2 cut(s) 207, 330
BssSI CACGAG 4 cut(s) 1141, 3063, 4544, 4745
BssT1I CCWWGG 9 cut(s) 19, 258, 514, 1725, 3480, 3831, 3862, 4296, 4719
Bst2BI CACGAG 4 cut(s) 1141, 3063, 4544, 4745
Bst2UI CCWGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
Bst6I CTCTTC 7 cut(s) 203, 302, 317, 359, 2158, 4909, 4985
BstACI GRCGYC 2 cut(s) 207, 330
BstAPI GCANNNNNTGC 2 cut(s) 2048, 4166
BstAUI TGTACA 1 cut(s) 4479
BstBI TTCGAA 2 cut(s) 1356, 2915
BstDSI CCRYGG 4 cut(s) 316, 3480, 3862, 4296
BstF5I GGATG 9 cut(s) 886, 1506, 1627, 2095, 2212, 2283, 2755, 4393, 4614
BstFNI CGCG 4 cut(s) 36, 318, 807, 1215
BstHHI GCGC 7 cut(s) 38, 93, 287, 1099, 2705, 3100, 3704
BstMCI CGRYCG 3 cut(s) 43, 408, 465
BstNI CCWGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
BstNSI RCATGY 4 cut(s) 1880, 2471, 2664, 5024
BstPAI GACNNNNGTC 1 cut(s) 3864
BstSCI CCNGG 8 cut(s) 86, 623, 770, 1957, 2202, 4270, 4315, 4883
BstSFI CTRYAG 6 cut(s) 1688, 2559, 3327, 4158, 4263, 4586
BstUI CGCG 4 cut(s) 36, 318, 807, 1215
BstV2I GAAGAC 2 cut(s) 574, 2261
BstX2I RGATCY 9 cut(s) 540, 1281, 1291, 2732, 3058, 3827, 4057, 4378, 4981
BstXI CCANNNNNNTGG 1 cut(s) 4726
BstYI RGATCY 9 cut(s) 540, 1281, 1291, 2732, 3058, 3827, 4057, 4378, 4981
BstZI CGGCCG 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 4344
BsuI GTATCC 1 cut(s) 2222
BsuTUI ATCGAT 1 cut(s) 4344
BtgI CCRYGG 4 cut(s) 316, 3480, 3862, 4296
BtgZI GCGATG 1 cut(s) 4536
BtrI CACGTC 1 cut(s) 3998
BtsCI GGATG 9 cut(s) 886, 1506, 1627, 2095, 2212, 2283, 2755, 4393, 4614
BtsI GCAGTG 3 cut(s) 1803, 4272, 4626
CaiI CAGNNNCTG 5 cut(s) 1516, 1649, 2048, 2324, 4271
CfoI GCGC 7 cut(s) 38, 93, 287, 1099, 2705, 3100, 3704
Cfr10I RCCGGY 1 cut(s) 38
Cfr42I CCGCGG 1 cut(s) 319
ClaI ATCGAT 1 cut(s) 4344
CseI GACGC 5 cut(s) 215, 338, 813, 2594, 3212
DraI TTTAAA 2 cut(s) 1678, 4245
DrdI GACNNNNNNGTC 1 cut(s) 1316
DriI GACNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 4 cut(s) 40, 319, 2349, 2394
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 7 cut(s) 203, 302, 317, 359, 2158, 4909, 4985
Eam1105I GACNNNNNGTC 1 cut(s) 211
EarI CTCTTC 7 cut(s) 203, 302, 317, 359, 2158, 4909, 4985
EciI GGCGGA 4 cut(s) 103, 115, 238, 4058
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco130I CCWWGG 9 cut(s) 19, 258, 514, 1725, 3480, 3831, 3862, 4296, 4719
Eco147I AGGCCT 1 cut(s) 3654
Eco24I GRGCYC 3 cut(s) 273, 414, 683
Eco31I GGTCTC 5 cut(s) 115, 314, 3138, 3470, 4095
Eco32I GATATC 2 cut(s) 4110, 4342
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
Eco57I CTGAAG 6 cut(s) 1016, 1814, 2262, 2618, 4014, 4023
Eco88I CYCGRG 1 cut(s) 1658
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 4 cut(s) 737, 1019, 2384, 4148
EcoRI GAATTC 1 cut(s) 2026
EcoRII CCWGG 8 cut(s) 86, 623, 770, 1957, 2202, 4270, 4315, 4883
EcoRV GATATC 2 cut(s) 4110, 4342
EcoT14I CCWWGG 9 cut(s) 19, 258, 514, 1725, 3480, 3831, 3862, 4296, 4719
EcoT22I ATGCAT 2 cut(s) 1878, 2518
EcoT38I GRGCYC 3 cut(s) 273, 414, 683
ErhI CCWWGG 9 cut(s) 19, 258, 514, 1725, 3480, 3831, 3862, 4296, 4719
Esp3I CGTCTC 1 cut(s) 4906
FaqI GGGAC 5 cut(s) 485, 544, 2892, 3976, 4122
FauI CCCGC 5 cut(s) 17, 280, 772, 1801, 1935
FauNDI CATATG 2 cut(s) 2845, 4411
FbaI TGATCA 3 cut(s) 952, 3748, 4357
FblI GTMKAC 1 cut(s) 243
FokI GGATG 9 cut(s) 893, 1513, 1614, 2102, 2219, 2270, 2762, 4400, 4601
FriOI GRGCYC 3 cut(s) 273, 414, 683
GlaI GCGC 7 cut(s) 37, 92, 286, 1098, 2704, 3099, 3703
GsaI CCCAGC 1 cut(s) 2383
GsuI CTGGAG 9 cut(s) 607, 843, 1109, 1217, 1410, 1755, 1980, 2874, 5028
HapII CCGG 2 cut(s) 39, 1447
HgaI GACGC 5 cut(s) 215, 338, 813, 2594, 3212
HhaI GCGC 7 cut(s) 38, 93, 287, 1099, 2705, 3100, 3704
Hin1I GRCGYC 2 cut(s) 207, 330
Hin6I GCGC 7 cut(s) 36, 91, 285, 1097, 2703, 3098, 3702
HinP1I GCGC 7 cut(s) 36, 91, 285, 1097, 2703, 3098, 3702
HincII GTYRAC 5 cut(s) 244, 1321, 1369, 1474, 2584
HindII GTYRAC 5 cut(s) 244, 1321, 1369, 1474, 2584
HindIII AAGCTT 1 cut(s) 2980
HpaI GTTAAC 1 cut(s) 1474
HpaII CCGG 2 cut(s) 39, 1447
Hpy99I CGWCG 6 cut(s) 191, 209, 245, 269, 332, 401
HpyCH4IV ACGT 4 cut(s) 612, 1666, 3997, 4953
HpySE526I ACGT 4 cut(s) 612, 1666, 3997, 4953
Hsp92I GRCGYC 2 cut(s) 207, 330
HspAI GCGC 7 cut(s) 36, 91, 285, 1097, 2703, 3098, 3702
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 3 cut(s) 952, 3748, 4357
KspAI GTTAAC 1 cut(s) 1474
KspI CCGCGG 1 cut(s) 319
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 8 cut(s) 678, 686, 766, 988, 1128, 1493, 2647, 3256
MaeII ACGT 4 cut(s) 612, 1666, 3997, 4953
MfeI CAATTG 2 cut(s) 1580, 3429
MflI RGATCY 9 cut(s) 540, 1281, 1291, 2732, 3058, 3827, 4057, 4378, 4981
MhlI GDGCHC 5 cut(s) 273, 414, 683, 2652, 5001
MlsI TGGCCA 1 cut(s) 2351
MluI ACGCGT 1 cut(s) 1213
MluNI TGGCCA 1 cut(s) 2351
MmeI TCCRAC 4 cut(s) 177, 966, 2488, 3077
Mox20I TGGCCA 1 cut(s) 2351
Mph1103I ATGCAT 2 cut(s) 1878, 2518
MroNI GCCGGC 1 cut(s) 38
MscI TGGCCA 1 cut(s) 2351
MslI CAYNNNNRTG 3 cut(s) 590, 1881, 2528
Msp20I TGGCCA 1 cut(s) 2351
MspA1I CMGCKG 5 cut(s) 318, 800, 2816, 4034, 4508
MspI CCGG 2 cut(s) 39, 1447
MspR9I CCNGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
MunI CAATTG 2 cut(s) 1580, 3429
Mva1269I GAATGC 3 cut(s) 287, 2399, 2502
MvaI CCWGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
MvnI CGCG 4 cut(s) 36, 318, 807, 1215
NaeI GCCGGC 1 cut(s) 40
NcoI CCATGG 3 cut(s) 3480, 3862, 4296
NdeI CATATG 2 cut(s) 2845, 4411
NgoMIV GCCGGC 1 cut(s) 38
NmeAIII GCCGAG 4 cut(s) 30, 235, 372, 3404
NmuCI GTSAC 7 cut(s) 613, 933, 1550, 1832, 2338, 2407, 2521
NsiI ATGCAT 2 cut(s) 1878, 2518
NspI RCATGY 4 cut(s) 1880, 2471, 2664, 5024
NspV TTCGAA 2 cut(s) 1356, 2915
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 1880
PaeR7I CTCGAG 1 cut(s) 1658
PceI AGGCCT 1 cut(s) 3654
PciI ACATGT 1 cut(s) 5020
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 7 cut(s) 138, 213, 261, 405, 414, 1212, 3983
PctI GAATGC 3 cut(s) 287, 2399, 2502
PdiI GCCGGC 1 cut(s) 40
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 1403
Ple19I CGATCG 2 cut(s) 408, 465
PpuMI RGGWCCY 4 cut(s) 737, 1019, 2384, 4148
PscI ACATGT 1 cut(s) 5020
PshAI GACNNNNGTC 1 cut(s) 3864
PshBI ATTAAT 3 cut(s) 2901, 3344, 4175
PsiI TTATAA 2 cut(s) 2276, 3105
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp5II RGGWCCY 4 cut(s) 737, 1019, 2384, 4148
Psp6I CCWGG 8 cut(s) 86, 623, 770, 1957, 2202, 4270, 4315, 4883
PspFI CCCAGC 1 cut(s) 2379
PspGI CCWGG 8 cut(s) 86, 623, 770, 1957, 2202, 4270, 4315, 4883
PspPPI RGGWCCY 4 cut(s) 737, 1019, 2384, 4148
PstI CTGCAG 5 cut(s) 1692, 2563, 4162, 4267, 4590
PstNI CAGNNNCTG 5 cut(s) 1516, 1649, 2048, 2324, 4271
PsuI RGATCY 9 cut(s) 540, 1281, 1291, 2732, 3058, 3827, 4057, 4378, 4981
PsyI GACNNNGTC 1 cut(s) 371
PvuI CGATCG 2 cut(s) 408, 465
PvuII CAGCTG 3 cut(s) 2816, 4034, 4508
RseI CAYNNNNRTG 3 cut(s) 590, 1881, 2528
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 1 cut(s) 319
SalI GTCGAC 1 cut(s) 242
SapI GCTCTTC 1 cut(s) 302
ScaI AGTACT 1 cut(s) 944
ScrFI CCNGG 8 cut(s) 88, 625, 772, 1959, 2204, 4272, 4317, 4885
SduI GDGCHC 5 cut(s) 273, 414, 683, 2652, 5001
SfcI CTRYAG 6 cut(s) 1688, 2559, 3327, 4158, 4263, 4586
Sfr274I CTCGAG 1 cut(s) 1658
Sfr303I CCGCGG 1 cut(s) 319
SfuI TTCGAA 2 cut(s) 1356, 2915
SgrBI CCGCGG 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 1658
SmiMI CAYNNNNRTG 3 cut(s) 590, 1881, 2528
SmlI CTYRAG 5 cut(s) 422, 1220, 1658, 4288, 4382
SmoI CTYRAG 5 cut(s) 422, 1220, 1658, 4288, 4382
SpeI ACTAGT 2 cut(s) 2036, 2855
SphI GCATGC 1 cut(s) 1880
SseBI AGGCCT 1 cut(s) 3654
SspI AATATT 2 cut(s) 978, 3204
SstI GAGCTC 2 cut(s) 414, 683
StuI AGGCCT 1 cut(s) 3654
StyD4I CCNGG 8 cut(s) 86, 623, 770, 1957, 2202, 4270, 4315, 4883
StyI CCWWGG 9 cut(s) 19, 258, 514, 1725, 3480, 3831, 3862, 4296, 4719
TaiI ACGT 4 cut(s) 615, 1669, 4000, 4956
TaqII GACCGA 4 cut(s) 296, 622, 1521, 1995
TatI WGTACW 6 cut(s) 942, 1624, 3148, 4010, 4479, 4787
TseFI GTSAC 7 cut(s) 613, 933, 1550, 1832, 2338, 2407, 2521
Tsp45I GTSAC 7 cut(s) 613, 933, 1550, 1832, 2338, 2407, 2521
TspGWI ACGGA 4 cut(s) 205, 361, 2059, 2102
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 1403
VspI ATTAAT 3 cut(s) 2901, 3344, 4175
XceI RCATGY 4 cut(s) 1880, 2471, 2664, 5024
XcmI CCANNNNNNNNNTGG 2 cut(s) 556, 4836
XhoI CTCGAG 1 cut(s) 1658
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 243
ZrmI AGTACT 1 cut(s) 944
Zsp2I ATGCAT 2 cut(s) 1878, 2518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.