pycom05g13800

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002933
Physical Location & Seq
Forward (+)
17342745 .. 17346720
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13800.4

Sequence Viewer

Length: 2112 bp
ATGTCCGCCTCCCAAACCCTAGGCGGCCCTTCCCGCGCCGGCCGCCTCCTCGGCCCCTCCCTCGACAAAATCATCAAGAACGCCGCCTGGCGCAAGCGCTCCCATTTGGTCTCCGCCTGCAAATCCGCCCTCGACAAGCTCGATTCCGTCACCGACTCCTCCATTATCGATCCAAAGTCACCCATATCCGGAATCTCTCTTTCCGACGCCGAGTTCGCCCTCGGCCCCGTCCTCCTCGCCTTAGATTCAGCTTACCCCAAGGTCGTCGAGCCCGCCATCGACTGCGTCTACAAGCTCTTCTCCCTCGGCATCATCCACGGCGAGATCGACACTTCGGATCCCAAGCTTTTGCTTTTCAAGCTCGTCGAGTCGGTGTGTAAGTGCGCGGCGATCAGCGAGGAGCCGATCGAGCTCGGCGTGCTTCGGGTCTTGCTCGCCGCCGTGAGATCGCCGCGGGTTTTCATCCGGGGCGATTGTTTGGTGAATATTGTGAGGACCTGTTACAATGTGTACCTTCGTGGAGTGAATGGGACCAATCAGATCTGCGCCAAGTCTGTTTTGGCTCAGATTATGGTGATTGTTTTCACCAGAGTGGAGGAGGATTCGGTGAATGTCAGCATTTCTAGGGTTTCGGTGAACGAATTGTTGGAATTCACTGATAAGAATTTGAACGAAGGGAGCTCTATTTTTTTCTGCCAGAATTTCGTCAATGAGATTATGGATGCGAGCTATGGCGGTCCAGATGGTATAAAGACGTTTGCCTTCCCGGTTCTCAAATTACAAAACGGCAGTGCCGATGGGGAGTCGAATAGTGATGGAGTTGAATCCGGTGACGGTTTTAGTAAGATTAGGGATGATGGGTATCTTTTGTTTAAGAATCTGTGTAAGTTGTCCATGAAATACTCATCACAGGAGCACTCTGATGATCAAATTCTCTTGAGGGGTAAAGTATTGTCCTTGGAGCTTTTGAAGGTGGTCATGGACAATGGGGGTCCAATCTGGCGCAACAATGAGAGGTTTCTCAATGCGATCAAGCAGTTCCTCTGCTTATCACTGTTAAAAAACAGTGCATTGTCAGTCATGGCCATTTTCCAGCTTCAGTGCTCTATTTTTACCAGCTTGTTGTCGAAATTCAGATCAGGGTTGAAAGCAGAAATTGGTATATTTTTTCCCATGCTTGTCCTACGCGTGCTTGAGAATGTCCTCCAGCCTAGTTTCTTGCAAAAAATGACAGTCCTCAATTTATTGGAGAAGATCTCTCAGGATTCGCAGATAATCATTGACATTTTTGTCAACTTTGACTGTGATGTGGATGCCCCAAACATCTTTGAAAGGATTGTCAATGGCCTTCTGAAAACTGCTCTAGGACCTCCCACTGGTTCAACAACAACATTGTCTCCAGTCCAAGACATCACGTTCCGGCATGAGTCAGTAAAGTGCTTGGTTAGCATCATCAATTCAATGGGATCTTGGATGGACCGACAGCTGAGTCTGGGAGGCTCCTATCTACCAAAGACCAATGAGAGTGACACTTCAGCTGAGAAAACAGAAAGTTTGACCCCAAATGGTGAAGAAGGAGCTGCTTTTGATAACGAAGTACATCCAGAAGGAAATCCTGAAGTTTCAGATGCTGCAACTCTTGAGCAACGTCGAGCTTATAAGCTTGAACTCCAGAAAGGTGTCACTCTGTTTAATAGGAAGCCTAACAAGGGCATTGAGTTTCTGATAAGCACCAAAAAAGTTGGTAGTTCCCCAGAAGATGTGGCTTCTTTCCTGAGGAACAACACTGCTGGCCTAAATGAAACCATGATTGGTGATTATTTAGGTGAAAGGGAAGAATTTCCACTAAAAGTTATGCATGCTTATGTGGATTCCTTTAATTTCAAAGGGATGGATTTCGGTGAAGCAATAAGGTTTTTCCTGAGGGGCTTCAGGTTACCTGGAGAAGCACAGAAAATTGACCGCATCATGGAGAAGTTTGCTGAGCGTTATTGTAAATGCAGTCCAAATTCTTTTACAAGTGCAGATACTGCATATGTGCTTGCATACTCTGTGATACTACTCAATACAGATGCCCATAATAACACGGTGAAAGATAAGGACCAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

704

Amino Acids

77.43

Weight (kDa)

5.51

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1659
AasI GACNNNNNNGTC 2 cut(s) 1289, 1488
AccI GTMKAC 1 cut(s) 288
AccII CGCG 4 cut(s) 36, 386, 454, 1188
AccIII TCCGGA 1 cut(s) 188
AclWI GGATC 4 cut(s) 164, 332, 345, 1474
AcoI YGGCCR 2 cut(s) 40, 1083
AcsI RAATTY 7 cut(s) 650, 664, 700, 930, 1130, 1838, 2008
AcuI CTGAAG 4 cut(s) 1082, 1518, 1638, 1915
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 512, 1599
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 4 cut(s) 188, 1376, 1709, 1969
AflIII ACRYGT 1 cut(s) 1186
AjnI CCWGG 2 cut(s) 86, 1939
AjuI GAANNNNNNNTTGG 4 cut(s) 629, 661, 1794, 1826
AleI CACNNNNGTG 1 cut(s) 590
Alw21I GWGCWC 4 cut(s) 414, 683, 918, 1106
Alw26I GTCTC 2 cut(s) 115, 1401
AlwI GGATC 4 cut(s) 164, 332, 345, 1474
AlwNI CAGNNNCTG 2 cut(s) 1631, 2030
Aor13HI TCCGGA 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 7 cut(s) 25, 40, 52, 223, 1083, 1345, 1792
ApeKI GCWGC 2 cut(s) 1580, 1631
ApoI RAATTY 7 cut(s) 650, 664, 700, 930, 1130, 1838, 2008
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp700I GAANNNNTTC 1 cut(s) 1839
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 6 cut(s) 38, 93, 99, 386, 548, 1005
AsuC2I CCSGG 2 cut(s) 467, 767
AvaII GGWCC 7 cut(s) 495, 531, 737, 992, 1367, 1477, 2101
AvrII CCTAGG 1 cut(s) 19
AxyI CCTNAGG 2 cut(s) 1775, 1922
BalI TGGCCA 1 cut(s) 1085
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 3 cut(s) 273, 414, 683
Bbv12I GWGCWC 4 cut(s) 414, 683, 918, 1106
BbvI GCAGC 2 cut(s) 1567, 1618
BccI CCATC 7 cut(s) 284, 737, 791, 809, 851, 1468, 1885
BceAI ACGGC 3 cut(s) 334, 425, 802
BciT130I CCWGG 2 cut(s) 88, 1941
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 2 cut(s) 467, 767
BcoDI GTCTC 2 cut(s) 115, 1401
BfaI CTAG 4 cut(s) 20, 624, 1212, 1364
BfoI RGCGCY 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 2 cut(s) 540, 1254
BisI GCNGC 8 cut(s) 25, 43, 84, 387, 438, 452, 1581, 1632
BlnI CCTAGG 1 cut(s) 19
BlpI GCTNAGC 1 cut(s) 1983
BlsI GCNGC 8 cut(s) 26, 44, 85, 388, 439, 453, 1582, 1633
Bme1390I CCNGG 4 cut(s) 88, 467, 767, 1941
Bme18I GGWCC 7 cut(s) 495, 531, 737, 992, 1367, 1477, 2101
BmiI GGNNCC 7 cut(s) 55, 226, 339, 402, 532, 993, 1501
BmrFI CCNGG 4 cut(s) 88, 467, 767, 1941
BmsI GCATC 7 cut(s) 318, 712, 1303, 1458, 1618, 1974, 2062
BplI GAGNNNNNCTC 2 cut(s) 1241, 1273
BpmI CTGGAG 4 cut(s) 1190, 1383, 1655, 1962
Bpu1102I GCTNAGC 1 cut(s) 1983
BpuEI CTTGAG 3 cut(s) 958, 1214, 1661
BpuMI CCSGG 2 cut(s) 467, 767
Bsa29I ATCGAT 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 861
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 115
BsaWI WCCGGW 2 cut(s) 188, 827
BsaXI ACNNNNNCTCC 2 cut(s) 1381, 1411
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 1376, 1709, 1969
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 2 cut(s) 1381, 1400
Bse21I CCTNAGG 2 cut(s) 1775, 1922
Bse8I GATNNNNATC 1 cut(s) 861
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 2 cut(s) 88, 1941
BseCI ATCGAT 1 cut(s) 168
BseGI GGATG 8 cut(s) 312, 462, 727, 859, 1318, 1479, 1600, 1896
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 4 cut(s) 188, 1376, 1709, 1969
BseMII CTCAG 7 cut(s) 578, 1274, 1478, 1530, 1766, 1913, 1974
BseNI ACTGG 2 cut(s) 1381, 1400
BseRI GAGGAG 5 cut(s) 38, 148, 224, 413, 611
BseX3I CGGCCG 1 cut(s) 40
BseXI GCAGC 2 cut(s) 1567, 1618
BsgI GTGCAG 1 cut(s) 2043
Bsh1236I CGCG 4 cut(s) 36, 386, 454, 1188
Bsh1285I CGRYCG 2 cut(s) 43, 408
BshFI GGCC 7 cut(s) 27, 42, 54, 225, 1085, 1347, 1794
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 2 cut(s) 43, 408
BsiHKAI GWGCWC 4 cut(s) 414, 683, 918, 1106
BsiSI CCGG 6 cut(s) 39, 189, 466, 767, 828, 1420
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 4 cut(s) 188, 1376, 1709, 1969
BsmAI GTCTC 2 cut(s) 115, 1401
BsmFI GGGAC 1 cut(s) 544
BsnI GGCC 7 cut(s) 27, 42, 54, 225, 1085, 1347, 1794
Bso31I GGTCTC 1 cut(s) 115
Bsp1286I GDGCHC 5 cut(s) 273, 414, 683, 918, 1106
Bsp13I TCCGGA 1 cut(s) 188
Bsp1720I GCTNAGC 1 cut(s) 1983
BspANI GGCC 7 cut(s) 27, 42, 54, 225, 1085, 1347, 1794
BspCNI CTCAG 7 cut(s) 577, 1273, 1479, 1531, 1767, 1914, 1975
BspDI ATCGAT 1 cut(s) 168
BspEI TCCGGA 1 cut(s) 188
BspFNI CGCG 4 cut(s) 36, 386, 454, 1188
BspLI GGNNCC 7 cut(s) 55, 226, 339, 402, 532, 993, 1501
BspPI GGATC 4 cut(s) 164, 332, 345, 1474
BspQI GCTCTTC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 2 cut(s) 1381, 1400
BssAI RCCGGY 1 cut(s) 38
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 4 cut(s) 19, 258, 957, 2104
Bst2UI CCWGG 2 cut(s) 88, 1941
Bst4CI ACNGT 6 cut(s) 836, 1056, 1067, 1234, 1304, 2089
Bst6I CTCTTC 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 2030
BstDEI CTNAG 8 cut(s) 241, 564, 1260, 1487, 1539, 1775, 1922, 1983
BstDSI CCRYGG 2 cut(s) 316, 452
BstEII GGTNACC 1 cut(s) 1935
BstF5I GGATG 8 cut(s) 312, 462, 727, 859, 1318, 1479, 1600, 1896
BstFNI CGCG 4 cut(s) 36, 386, 454, 1188
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 6 cut(s) 38, 93, 99, 386, 548, 1005
BstMAI GTCTC 2 cut(s) 115, 1401
BstMCI CGRYCG 2 cut(s) 43, 408
BstMWI GCNNNNNNNGC 9 cut(s) 33, 42, 51, 215, 358, 409, 418, 1446, 2030
BstNI CCWGG 2 cut(s) 88, 1941
BstNSI RCATGY 1 cut(s) 1862
BstPI GGTNACC 1 cut(s) 1935
BstSCI CCNGG 4 cut(s) 86, 465, 765, 1939
BstUI CGCG 4 cut(s) 36, 386, 454, 1188
BstV1I GCAGC 2 cut(s) 1567, 1618
BstX2I RGATCY 4 cut(s) 337, 540, 1254, 1466
BstYI RGATCY 4 cut(s) 337, 540, 1254, 1466
BstZI CGGCCG 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 2 cut(s) 1775, 1922
BsuRI GGCC 7 cut(s) 27, 42, 54, 225, 1085, 1347, 1794
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 2 cut(s) 316, 452
BtsCI GGATG 8 cut(s) 312, 462, 727, 859, 1318, 1479, 1600, 1896
BtsI GCAGTG 2 cut(s) 796, 1785
BtsIMutI CAGTG 7 cut(s) 654, 796, 1052, 1072, 1106, 1374, 1785
CaiI CAGNNNCTG 2 cut(s) 1631, 2030
CfoI GCGC 6 cut(s) 38, 93, 99, 386, 548, 1005
Cfr10I RCCGGY 1 cut(s) 38
Cfr42I CCGCGG 1 cut(s) 455
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 2 cut(s) 215, 274
Csp6I GTAC 2 cut(s) 511, 1598
CviAII CATG 8 cut(s) 895, 979, 1081, 1174, 1424, 1807, 1859, 1969
CviQI GTAC 2 cut(s) 511, 1598
DdeI CTNAG 8 cut(s) 241, 564, 1260, 1487, 1539, 1775, 1922, 1983
DrdI GACNNNNNNGTC 2 cut(s) 1289, 1488
DseDI GACNNNNNNGTC 2 cut(s) 1289, 1488
EaeI YGGCCR 2 cut(s) 40, 1083
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
EciI GGCGGA 2 cut(s) 103, 115
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco130I CCWWGG 4 cut(s) 19, 258, 957, 2104
Eco24I GRGCYC 3 cut(s) 273, 414, 683
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 7 cut(s) 495, 531, 737, 992, 1367, 1477, 2101
Eco47III AGCGCT 1 cut(s) 98
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
Eco57I CTGAAG 4 cut(s) 1082, 1518, 1638, 1915
Eco81I CCTNAGG 2 cut(s) 1775, 1922
Eco91I GGTNACC 1 cut(s) 1935
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 2 cut(s) 495, 1367
EcoO65I GGTNACC 1 cut(s) 1935
EcoRI GAATTC 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 86, 1939
EcoT14I CCWWGG 4 cut(s) 19, 258, 957, 2104
EcoT22I ATGCAT 1 cut(s) 1860
EcoT38I GRGCYC 3 cut(s) 273, 414, 683
ErhI CCWWGG 4 cut(s) 19, 258, 957, 2104
FaeI CATG 8 cut(s) 898, 982, 1084, 1177, 1427, 1810, 1862, 1972
FaqI GGGAC 1 cut(s) 544
FatI CATG 8 cut(s) 894, 978, 1080, 1173, 1423, 1806, 1858, 1968
FauI CCCGC 3 cut(s) 41, 280, 447
FauNDI CATATG 1 cut(s) 2035
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 8 cut(s) 25, 43, 84, 387, 438, 452, 1581, 1632
FokI GGATG 8 cut(s) 299, 449, 734, 866, 1325, 1486, 1587, 1903
FriOI GRGCYC 3 cut(s) 273, 414, 683
Fsp4HI GCNGC 8 cut(s) 25, 43, 84, 387, 438, 452, 1581, 1632
FspBI CTAG 4 cut(s) 20, 624, 1212, 1364
GlaI GCGC 6 cut(s) 37, 92, 98, 385, 547, 1004
GluI GCNGC 8 cut(s) 25, 43, 84, 387, 438, 452, 1581, 1632
GsuI CTGGAG 4 cut(s) 1190, 1383, 1655, 1962
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 7 cut(s) 27, 42, 54, 225, 1085, 1347, 1794
HapII CCGG 6 cut(s) 39, 189, 466, 767, 828, 1420
HgaI GACGC 2 cut(s) 215, 274
HhaI GCGC 6 cut(s) 38, 93, 99, 386, 548, 1005
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 8 cut(s) 898, 982, 1084, 1177, 1427, 1810, 1862, 1972
Hin6I GCGC 6 cut(s) 36, 91, 97, 384, 546, 1003
HinP1I GCGC 6 cut(s) 36, 91, 97, 384, 546, 1003
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HindIII AAGCTT 2 cut(s) 344, 1661
HpaII CCGG 6 cut(s) 39, 189, 466, 767, 828, 1420
Hpy166II GTNNAC 4 cut(s) 289, 511, 637, 1294
Hpy188I TCNGA 9 cut(s) 205, 337, 540, 567, 922, 1136, 1353, 1627, 1725
Hpy8I GTNNAC 4 cut(s) 289, 511, 637, 1294
Hpy99I CGWCG 4 cut(s) 209, 269, 368, 1653
HpyAV CCTTC 8 cut(s) 39, 524, 668, 772, 964, 1358, 1568, 1601
HpyCH4III ACNGT 6 cut(s) 836, 1056, 1067, 1234, 1304, 2089
HpyCH4IV ACGT 3 cut(s) 755, 1415, 1648
HpyCH4V TGCA 9 cut(s) 120, 1070, 1222, 1634, 1858, 2001, 2024, 2033, 2045
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 42, 51, 215, 358, 409, 418, 1446, 2030
HpyF3I CTNAG 8 cut(s) 241, 564, 1260, 1487, 1539, 1775, 1922, 1983
HpySE526I ACGT 3 cut(s) 755, 1415, 1648
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 8 cut(s) 898, 982, 1084, 1177, 1427, 1810, 1862, 1972
HspAI GCGC 6 cut(s) 36, 91, 97, 384, 546, 1003
Kpn2I TCCGGA 1 cut(s) 188
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 925
KspI CCGCGG 1 cut(s) 455
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 7 cut(s) 104, 400, 678, 913, 961, 1505, 1577
Lsp1109I GCAGC 2 cut(s) 1567, 1618
LweI GCATC 7 cut(s) 318, 712, 1303, 1458, 1618, 1974, 2062
MaeI CTAG 4 cut(s) 20, 624, 1212, 1364
MaeII ACGT 3 cut(s) 755, 1415, 1648
MaeIII GTNAC 7 cut(s) 148, 177, 500, 830, 1526, 1681, 1935
MboII GAAGA 5 cut(s) 289, 1264, 1583, 1769, 1847
MflI RGATCY 4 cut(s) 337, 540, 1254, 1466
MhlI GDGCHC 5 cut(s) 273, 414, 683, 918, 1106
MlsI TGGCCA 1 cut(s) 1085
MluI ACGCGT 1 cut(s) 1186
MluNI TGGCCA 1 cut(s) 1085
MlyI GAGTC 5 cut(s) 149, 377, 812, 1436, 1498
MmeI TCCRAC 2 cut(s) 228, 627
Mox20I TGGCCA 1 cut(s) 1085
Mph1103I ATGCAT 1 cut(s) 1860
MroI TCCGGA 1 cut(s) 188
MroNI GCCGGC 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 1839
MscI TGGCCA 1 cut(s) 1085
MseI TTAA 4 cut(s) 873, 1058, 1692, 1878
MslI CAYNNNNRTG 3 cut(s) 590, 921, 1863
Msp20I TGGCCA 1 cut(s) 1085
MspA1I CMGCKG 3 cut(s) 454, 1486, 1538
MspI CCGG 6 cut(s) 39, 189, 466, 767, 828, 1420
MspR9I CCNGG 4 cut(s) 88, 467, 767, 1941
MvaI CCWGG 2 cut(s) 88, 1941
MvnI CGCG 4 cut(s) 36, 386, 454, 1188
MwoI GCNNNNNNNGC 9 cut(s) 33, 42, 51, 215, 358, 409, 418, 1446, 2030
NaeI GCCGGC 1 cut(s) 40
NciI CCSGG 2 cut(s) 467, 767
NdeI CATATG 1 cut(s) 2035
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 8 cut(s) 898, 982, 1084, 1177, 1427, 1810, 1862, 1972
NlaIV GGNNCC 7 cut(s) 55, 226, 339, 402, 532, 993, 1501
NmeAIII GCCGAG 5 cut(s) 30, 201, 235, 285, 393
NmuCI GTSAC 5 cut(s) 148, 177, 830, 1526, 1681
NsiI ATGCAT 1 cut(s) 1860
NspI RCATGY 1 cut(s) 1862
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 1862
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 324, 414, 792
PdiI GCCGGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 1839
PfeI GAWTC 8 cut(s) 143, 192, 245, 602, 824, 877, 1265, 1871
PflFI GACNNNGTC 1 cut(s) 284
PkrI GCNGC 8 cut(s) 26, 44, 85, 388, 439, 453, 1582, 1633
Ple19I CGATCG 1 cut(s) 408
PleI GAGTC 5 cut(s) 149, 376, 811, 1435, 1497
PpsI GAGTC 5 cut(s) 149, 376, 811, 1435, 1497
PpuMI RGGWCCY 2 cut(s) 495, 1367
PsiI TTATAA 1 cut(s) 1659
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp5II RGGWCCY 2 cut(s) 495, 1367
Psp6I CCWGG 2 cut(s) 86, 1939
PspEI GGTNACC 1 cut(s) 1935
PspGI CCWGG 2 cut(s) 86, 1939
PspN4I GGNNCC 7 cut(s) 55, 226, 339, 402, 532, 993, 1501
PspPPI RGGWCCY 2 cut(s) 495, 1367
PstNI CAGNNNCTG 2 cut(s) 1631, 2030
PsuI RGATCY 4 cut(s) 337, 540, 1254, 1466
PsyI GACNNNGTC 1 cut(s) 284
PvuI CGATCG 1 cut(s) 408
PvuII CAGCTG 2 cut(s) 1486, 1538
RsaI GTAC 2 cut(s) 512, 1599
RsaNI GTAC 2 cut(s) 511, 1598
RseI CAYNNNNRTG 3 cut(s) 590, 921, 1863
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 4 cut(s) 873, 1058, 1692, 1878
SatI GCNGC 8 cut(s) 25, 43, 84, 387, 438, 452, 1581, 1632
SchI GAGTC 5 cut(s) 149, 377, 812, 1436, 1498
ScrFI CCNGG 4 cut(s) 88, 467, 767, 1941
SduI GDGCHC 5 cut(s) 273, 414, 683, 918, 1106
SfaNI GCATC 7 cut(s) 318, 712, 1303, 1458, 1618, 1974, 2062
Sfr303I CCGCGG 1 cut(s) 455
SgrBI CCGCGG 1 cut(s) 455
SinI GGWCC 7 cut(s) 495, 531, 737, 992, 1367, 1477, 2101
SmiMI CAYNNNNRTG 3 cut(s) 590, 921, 1863
SmlI CTYRAG 3 cut(s) 937, 1193, 1640
SmoI CTYRAG 3 cut(s) 937, 1193, 1640
SphI GCATGC 1 cut(s) 1862
SspI AATATT 1 cut(s) 487
SspMI CTAG 4 cut(s) 20, 624, 1212, 1364
SstI GAGCTC 2 cut(s) 414, 683
StyD4I CCNGG 4 cut(s) 86, 465, 765, 1939
StyI CCWWGG 4 cut(s) 19, 258, 957, 2104
TaaI ACNGT 6 cut(s) 836, 1056, 1067, 1234, 1304, 2089
TaiI ACGT 3 cut(s) 758, 1418, 1651
TaqII GACCGA 1 cut(s) 1494
TatI WGTACW 1 cut(s) 1597
TauI GCSGC 6 cut(s) 27, 45, 86, 389, 440, 454
TfiI GAWTC 8 cut(s) 143, 192, 245, 602, 824, 877, 1265, 1871
Tru1I TTAA 4 cut(s) 873, 1058, 1692, 1878
Tru9I TTAA 4 cut(s) 873, 1058, 1692, 1878
TscAI CASTG 7 cut(s) 661, 796, 1059, 1072, 1106, 1381, 1792
TseFI GTSAC 5 cut(s) 148, 177, 830, 1526, 1681
TseI GCWGC 2 cut(s) 1580, 1631
Tsp45I GTSAC 5 cut(s) 148, 177, 830, 1526, 1681
TspDTI ATGAA 3 cut(s) 451, 911, 1815
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 7 cut(s) 661, 796, 1059, 1072, 1106, 1381, 1792
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 7 cut(s) 495, 531, 737, 992, 1367, 1477, 2101
XapI RAATTY 7 cut(s) 650, 664, 700, 930, 1130, 1838, 2008
XceI RCATGY 1 cut(s) 1862
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 1839
XspI CTAG 4 cut(s) 20, 624, 1212, 1364
Zsp2I ATGCAT 1 cut(s) 1860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.