Rh6BG255200

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002933
Physical Location & Seq
Forward (+)
46763329 .. 46774062
10734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG255200.1

Sequence Viewer

Length: 5163 bp
ATGTCGGCTTCTCAAACCCTAGGCGGGGTTTCACGCGCCGGCCGCCTCCTCGGCCCCTCCCTCGACAAAATCATCAAGAACGCCGCCTGGCGCAAGCACTCCCATTTGGTCTCCGCCTGCAAATCCGCCCTCGACAAGCTCGACTCCCTCTCCGACTCCCCCCTCAACGACCCCAACTCCCCCATCGTCGGCTTCCTCTTCCCCGACGCCGAGTCCGTCCTCGCCCCCCTCCTCCTCGCCGTCGACTCCGCCCACGCCAAGGTCGTCGAGCCCGCCCTCGAATGCGCCTTCAAGCTCTTCTCCCTCGGTCTCTTCCGCGGCCAGATCGACGCCGCCGCCCCCAAGTTCGTCCTCTTCAAGCTCATCGACTCCGTCTGCAAGTGCGGCGGCCTCGGCGACGAGGCGATCGAGCTCGCCGTCCTCAAGGCTCTGCTCGCCGCCGTTAGATCGCCGTTAGTGGCGATCAGAGGGGACTGCTTGGTGAGTATTGTGAGGACTTGTTACAATGTGTACCTTGGTGGATTGAATGGGACCAATCAGATCTGTGCCAAGTCGGTTTTGGCTCAGATTATGGTCATCGTCTTCACCAGAGTGGAGGCCGATGCTATGTACGTCACCATCTCCAGGGTTTCGGTCAGCGAATTGCTGGAATTTACTGATAAGAATCTGAATGAAGGGAGCTCCATTATGTTCTGCCAGAATTTCATCAATGAGGTTATGGAAGCTAGTTATGGGGGTCCTGATAGTATCAAAATGGCGGCTCCCGCCCCCAGGAGATTGCAAAATGGAAATGCCGCTGCTGGACGCGGTGAGTCGGGAGCTGGAGAATCCAATGATGAAGCCGAATCTGGCGAGGGAGGTGGTAGTAGTAAGATTCGGGATGATGGATTTCTTTTGTTTAAGAATTTGTGTAAGTTGTCGATGAAGTTCTCGTCACAGGAGTACTCTGATGATCAAATACTATTGAGGGGTAAAATATTGTCGTTGGAGCTTCTGAAGGTGGTTATGGACAATGGGGGTCCTATTTGGCTCTCCAATGACAGGTTTCTCAATGGCATCAAGCAGTTCCTATGCTTATCATTGCTAAAAAACAGTGCGCTGTCAGTTATGGCTATTTTCCAGCTCCAGTGTTCTATTTTCACGAGCTTGTTATCAAAATTTAGATCAGGGTTGAAAGCAGAAATCGGTATATTTTTTCCCATGCTCGTCTTACGCGTGCTTGAGAATGTTCTCCAGCCTAGTTTCTTGCAGAAAATGACGGTCCTCAATTTATTAGAGAAGGTCTCTCAGGATCCACAGATTATCATTGACATTTTTGTCAACTATGACTGTGATGTGGATTCTCCAAACATTTTCGAAAGGATTGTCAACGGCCTTCTGAAAACTGCTCCAGGTCCACCTTCTGGTTCAACAACAACGTTATCTCCAGTTCAGGATATTACTTTCCGGCATGAATCAGTGAAGTGCTTGGTTAACATCATTAAGTCAATGGGAGCTTGGATGGACCGACAGAGACTGGGAGACTCCTATCTGCCAAATACCAATGAGAGTGACACTACATCAGAGAAAACAGAGAATCAATTGACCCTAAACGGTGAAGAAGGAACCGCTTTAGACAATGAAGTACATCCAGAAGGAAGTTCAGATGCTGCAACTCTCGAGCAACGTCGAGCTTTTAAACTTGAACTGCAGAAAGGTATCTCACTGTTCAATAGGAAACCTTCCAAGGGCATTGAGTTTCTGATAAGCACAAAAAAAATTGGGGGTTCTCCAGCAGATGTGGCTTCTTTCCTGAAGAACAACACTGCGGGCCTAAATGAAACCATGATCGGTGACTATTTGGGTGAAAGGGAAGAATTTTCTCTAAAAGTTATGCATGCTTATGTAGATTCCTTTAATTTCAAAGGGATGGATTTTGGTGAAGCAATTAGGTTTTTCCTGCGGGGGTTCAAGTTACCTGGAGAAGCACAGAAAATTGACCGAATCATGGAGAAGTTTGCTGAGCGTTATTGTAAATGCAGTCCGAATTCATTTACTAGTGCAGATACTGCTTATGTCCTTGCATACTCTGTGATAATGCTCAACACGGATGCCCATAATATCATGGTTAAAGATAAGATGACCAAGGCCGATTTCATACGTAACAACCGAGGAATAGATGATGGCAAGGATCTACCTGAGGAATATCTTGGTGCACTATATGATCAGATTGTTAAAAATGAAATTAAGATGAAGGCTGACTCTTCTGTACCACAGAGCAAGCAGGAAAACAGCTTTAACAAATTATTGGGCCTGGATGGTATCCTGAATCTAGTAACTGGGAAGCAGACTGAAGAAAAAGCGTTGGGTGCAAATGGGCTTCTTATAAAGGACATCCAAGAGCAATTTAAGGCGAAGTCAGGGAAATCAGAGTCTGTTTATCATTCTGTGACAGATGTGGCCATATTGAGGTTCATGGTGGAGGCCTGCTGGGGTCCTATGCTGGCCGCATTCAGTGTGACTCTGGACCAAAGTGATGACAGGCTTGCTACTGCTCAATGCTTACTGGGTTTCCGACATGCAATTCATGTTACTGCTCTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACATCAATGGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAGAATGTTGACGCTGTGAAGGCAATAATAGCAATCGCCGTTGAAGATGGTAATCATCTTCAGGAAGCATGGGAGCACATATTAACATGCCTTTCCCGAATTGAGCATCTGCAACTGTTGGGTGAGGGCGCACCTACTGATGCAACCTTTTTCAGTGGATCTAATGTTGAAGCGGATGACAAATCACCAAAGCCTATTGGTTTTGCTTCTCTAAAGAAAAAAGGAACTATCCAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCATATGATAGCACTAGTATTGGAATCAACACTTCAGGACTGGTATCTCCAGAACAGATTAATAATTTTATTTCGAATTTGAATTTGCTGGATCAGATTGGGAATTTTGAGTTGAACCATGTATTTGCTCATAGCCAAAGCTTGAACAGTGAAGCCATAGTTGCTTTTGTGAAGTCCTTATGTAAAGTTTCCATGTCAGAATTGCAGTCTCCAACAGATCCTCGTGTTTTCAGCCTCACAAAAATAGTTGAAATAGCGCATTATAATATGAACCGCATCAGATTAGTGTGGTCTCGAATATGGAATGTACTGTCAGATTTCTTTGTATCAGTTGGCTTGTCAGAGAATCTTTCAGTTGCAATATTTGTGATGGATTCCTTGCGTCAGCTTGCTATGAAATTCTTAGAGCGTGAGGAGCTGGCAAATTACAATTTCCAGAACGAATTTTTAAGACCATTTGTGATTGTTATGCAGAAAAGCAGCTCTACAGAAATTAGGGAATTAATAGTTCGGTGCATTTCACAAATGGTCCTCAGCCGGGTCAATAATGTGAAATCTGGCTGGAAAAGTGTTTTTATGGTTTTCACAATTGCTGCTGCTGATGAGAGGAAAAATATAGTCTTGTTGGCCTTTGAGACCATGGAAAAGATAGTCAGAGAGTATTTCCCTTACATAACTGAGACAGAGACATTGACTTTTACTGATTGTGTTAGATGCCTCTTAACCTTCACAAATAGCAGATTCAATAGTGATGTTAGCCTCAATGCTATTGCGTTTCTGCGTTTCTGTGCCGTCAAACTTGCAGAAGGAGGCCTTGTTTACAATAAGAACAGTGAGATTGATGGTTCTTCAAGTCCTAGCGCAAGTGAAGGTGCTTCAACTGTTGAGAATTTCAATGATAAAGATGATCATACATCATTTTGGGTTCCATTGCTAACAGGTTTGTCCAAACTAACATCTGATCCTAGATCAGCTATCAGGAAAGGATCCTTGGAAGTTCTTTTCAACATTCTAAAGGACCATGGTCATCTTTTCTCACACCTGTTTTGGACTGCGGTTTTCAATTCTGTTATTTTTCCCATATTTAATGGTGTCAGCGATAAGAAAGATACAGACATGAAAAATGACCAGTCCTCGCCAATTTCGATGTCCCCACGTCCTGAAGGAAGTACATGGGATTCTGAAACTTCAGCTGTTGCGGCGGATTGCCTCATAGATCTGTTCGTCAGTTTCTTTGATGTTGTTAGGTCTCAACTACCAAGTGTGATATCCATTCTGACAGGACTCATCAGAAGTCCCATTCAGGGTCCTGCTACTGCAGGAGTTGCTGCATTAATGCGTTTGTCTAGTGAGGTGGGTAGCAGGCTTTCAGAGGATGAATGGAGAGCAATTTTTCTGGCTTTAAAGGAGGCTGCCACATCTGCAGTGCCTGGATTTTTGAAGGTCTTGAGAACCATGGATAATGTCAATGTTCCTGGTCTTTCTGAATCTTTTAGCGATATCGATATGTCGTCTGATCAGGGGTACTCAAATGAGGATCTTGAGGATGATAATTTGCAAACAGCTTCATATGTGGTTTCAAGAATCAAGAGTCATGTTTCTATGCAGCTACTCATCTTACAGGTCGCTGCTGATTTGTACAAAATAAACCTAGAAACCTTGTCAGCTGCTAACATTGCAGTTCTTCTTGAAGTATATTCTCTCGTGTCATCGCACGCTCACCAACTGAACTCTGAGACAATTCTGCAGAAGAAGTTGGAAAAAGTGTGTTCCATCCTAGAACTCACTGCACCACCTATTGTTCATTTTGAGAATGAATCTTACAAAAACTATCTTAACTTCCTCCAAAACGCACTTGTGGATAATCCATCTCTTTCCAAGGAAATGGACATAGAAGCTAAACTTGTGGCAGTCTGTGAAAACATATTGCAGATATACCTAAAGTGTACTGAGCTTCAATCTTCTGAGCAGAAGCCTACTGGTCAGCCAGTTTTGCACTGGATTCTTCCTTTGGGTACAGCAAAAAAGGAGGAATTGGCTACCAGGACTTTCTTAGCCGTCTCTTCATTGCAGGCATTGAGTGGTTTGGAAAAGGTTTCATTTAGGAGGCACGTTTCTCAGTTATTTCCTCTGTTGGTAGATCTTGTGAAGAGTGAGCACACTTCTGGGGAAGTTCAACATGTTCTAAGCAACATATTCCAGTCATGTATAGGCCCAATAATAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1720

Amino Acids

189.38

Weight (kDa)

5.52

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 19 - 201 3.3e-23 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 293 - 451 5.5e-42 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 560 - 742 1.1e-74 Sec7 domain
Sec7-like_HDS PF09324 1110 - 1192 2.5e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1282 - 1348 5.1e-06 C-terminal region of Mon2 protein
BIG2_C PF20252 1512 - 1711 9.7e-26 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2366, 3195
AasI GACNNNNNNGTC 1 cut(s) 1316
AccB7I CCANNNNNTGG 1 cut(s) 1403
AccI GTMKAC 1 cut(s) 243
AccII CGCG 4 cut(s) 36, 318, 807, 1215
AclI AACGTT 1 cut(s) 1418
AcoI YGGCCR 4 cut(s) 40, 319, 2439, 2484
AcuI CTGAAG 7 cut(s) 1016, 1814, 2352, 2708, 2949, 4104, 4113
AcyI GRCGYC 2 cut(s) 207, 330
AflIII ACRYGT 2 cut(s) 1213, 5110
AhdI GACNNNNNGTC 1 cut(s) 211
AhlI ACTAGT 2 cut(s) 2036, 2945
AjiI CACGTC 1 cut(s) 4088
AjnI CCWGG 9 cut(s) 86, 623, 770, 1390, 1957, 2292, 4360, 4405, 4973
AjuI GAANNNNNNNTTGG 4 cut(s) 2117, 2149, 3905, 3937
AleI CACNNNNGTG 1 cut(s) 590
Alw21I GWGCWC 5 cut(s) 414, 683, 2197, 2742, 5091
Alw44I GTGCAC 1 cut(s) 2193
AlwNI CAGNNNCTG 5 cut(s) 1516, 1649, 2048, 2414, 4361
Ama87I CYCGRG 1 cut(s) 1658
ApaLI GTGCAC 1 cut(s) 2193
ArsI GACNNNNNNTTYG 2 cut(s) 1350, 1382
AseI ATTAAT 3 cut(s) 2991, 3434, 4265
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 7 cut(s) 38, 93, 287, 1099, 2795, 3190, 3794
AsuC2I CCSGG 1 cut(s) 3470
AsuII TTCGAA 2 cut(s) 1356, 3005
AvaI CYCGRG 1 cut(s) 1658
AvrII CCTAGG 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 2178
BaeGI GKGCMC 1 cut(s) 2197
BalI TGGCCA 1 cut(s) 2441
BamHI GGATCC 2 cut(s) 1291, 3917
BanII GRGCYC 3 cut(s) 273, 414, 683
BauI CACGAG 3 cut(s) 1141, 3153, 4634
BbsI GAAGAC 1 cut(s) 574
Bbv12I GWGCWC 5 cut(s) 414, 683, 2197, 2742, 5091
BbvCI CCTCAGC 1 cut(s) 3464
BceAI ACGGC 8 cut(s) 224, 401, 425, 436, 1387, 2687, 3707, 4973
BciT130I CCWGG 9 cut(s) 88, 625, 772, 1392, 1959, 2294, 4362, 4407, 4975
BciVI GTATCC 1 cut(s) 2312
BclI TGATCA 4 cut(s) 952, 2203, 3838, 4447
BcnI CCSGG 1 cut(s) 3470
BcuI ACTAGT 2 cut(s) 2036, 2945
BfmI CTRYAG 6 cut(s) 1688, 2649, 3417, 4248, 4353, 4676
BfuI GTATCC 1 cut(s) 2312
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 3 cut(s) 540, 4147, 5071
BlnI CCTAGG 1 cut(s) 19
BlpI GCTNAGC 1 cut(s) 2001
BmcAI AGTACT 1 cut(s) 944
BmeRI GACNNNNNGTC 1 cut(s) 211
BmeT110I CYCGRG 1 cut(s) 1658
BmgBI CACGTC 1 cut(s) 4088
BmrI ACTGGG 3 cut(s) 1526, 2328, 2555
BmsI GCATC 9 cut(s) 592, 1065, 1636, 2080, 2593, 2779, 2794, 3216, 3635
BmuI ACTGGG 3 cut(s) 1526, 2328, 2555
BoxI GACNNNNGTC 1 cut(s) 3954
BpiI GAAGAC 1 cut(s) 574
BplI GAGNNNNNCTC 2 cut(s) 1268, 1300
BpmI CTGGAG 9 cut(s) 607, 843, 1109, 1217, 1374, 1410, 1755, 1980, 2964
Bpu10I CCTNAGC 1 cut(s) 3464
Bpu1102I GCTNAGC 1 cut(s) 2001
Bpu14I TTCGAA 2 cut(s) 1356, 3005
BpuEI CTTGAG 4 cut(s) 407, 1241, 4399, 4493
BpuMI CCSGG 1 cut(s) 3470
Bsa29I ATCGAT 1 cut(s) 4434
BsaAI YACGTR 1 cut(s) 2141
BsaBI GATNNNNATC 2 cut(s) 663, 2715
BsaHI GRCGYC 2 cut(s) 207, 330
BsaI GGTCTC 6 cut(s) 115, 314, 1288, 3228, 3560, 4185
BsaXI ACNNNNNCTCC 8 cut(s) 134, 161, 164, 191, 1408, 1438, 3732, 3762
Bse118I RCCGGY 1 cut(s) 38
Bse21I CCTNAGG 1 cut(s) 2178
Bse3DI GCAATG 4 cut(s) 1079, 3860, 4605, 4997
Bse8I GATNNNNATC 2 cut(s) 663, 2715
BseBI CCWGG 9 cut(s) 88, 625, 772, 1392, 1959, 2294, 4362, 4407, 4975
BseCI ATCGAT 1 cut(s) 4434
BseJI GATNNNNATC 2 cut(s) 663, 2715
BseMI GCAATG 4 cut(s) 1079, 3860, 4605, 4997
BseRI GAGGAG 4 cut(s) 38, 221, 224, 3359
BseSI GKGCMC 1 cut(s) 2197
BseX3I CGGCCG 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 2469
BsgI GTGCAG 2 cut(s) 2061, 4704
Bsh1236I CGCG 4 cut(s) 36, 318, 807, 1215
Bsh1285I CGRYCG 2 cut(s) 43, 408
BshVI ATCGAT 1 cut(s) 4434
BsiEI CGRYCG 2 cut(s) 43, 408
BsiHKAI GWGCWC 5 cut(s) 414, 683, 2197, 2742, 5091
BsiHKCI CYCGRG 1 cut(s) 1658
BsiSI CCGG 3 cut(s) 39, 1447, 3469
BslFI GGGAC 4 cut(s) 485, 544, 4066, 4212
BsmBI CGTCTC 1 cut(s) 4996
BsmFI GGGAC 4 cut(s) 485, 544, 4066, 4212
BsmI GAATGC 3 cut(s) 287, 2489, 2592
Bso31I GGTCTC 6 cut(s) 115, 314, 1288, 3228, 3560, 4185
BsoBI CYCGRG 1 cut(s) 1658
Bsp119I TTCGAA 2 cut(s) 1356, 3005
Bsp1286I GDGCHC 6 cut(s) 273, 414, 683, 2197, 2742, 5091
Bsp1407I TGTACA 1 cut(s) 4569
Bsp1720I GCTNAGC 1 cut(s) 2001
Bsp19I CCATGG 3 cut(s) 3570, 3952, 4386
BspDI ATCGAT 1 cut(s) 4434
BspFNI CGCG 4 cut(s) 36, 318, 807, 1215
BspMAI CTGCAG 5 cut(s) 1692, 2653, 4252, 4357, 4680
BspQI GCTCTTC 1 cut(s) 302
BspT104I TTCGAA 2 cut(s) 1356, 3005
BspTNI GGTCTC 6 cut(s) 115, 314, 1288, 3228, 3560, 4185
BsrDI GCAATG 4 cut(s) 1079, 3860, 4605, 4997
BsrFI RCCGGY 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 4569
BssAI RCCGGY 1 cut(s) 38
BssNI GRCGYC 2 cut(s) 207, 330
BssSI CACGAG 3 cut(s) 1141, 3153, 4634
Bst2BI CACGAG 3 cut(s) 1141, 3153, 4634
Bst2UI CCWGG 9 cut(s) 88, 625, 772, 1392, 1959, 2294, 4362, 4407, 4975
Bst6I CTCTTC 7 cut(s) 203, 302, 317, 359, 2248, 4999, 5075
BstACI GRCGYC 2 cut(s) 207, 330
BstAPI GCANNNNNTGC 2 cut(s) 2048, 4256
BstAUI TGTACA 1 cut(s) 4569
BstBAI YACGTR 1 cut(s) 2141
BstBI TTCGAA 2 cut(s) 1356, 3005
BstDSI CCRYGG 4 cut(s) 316, 3570, 3952, 4386
BstFNI CGCG 4 cut(s) 36, 318, 807, 1215
BstHHI GCGC 7 cut(s) 38, 93, 287, 1099, 2795, 3190, 3794
BstMCI CGRYCG 2 cut(s) 43, 408
BstNI CCWGG 9 cut(s) 88, 625, 772, 1392, 1959, 2294, 4362, 4407, 4975
BstNSI RCATGY 4 cut(s) 1880, 2561, 2754, 5114
BstPAI GACNNNNGTC 1 cut(s) 3954
BstSFI CTRYAG 6 cut(s) 1688, 2649, 3417, 4248, 4353, 4676
BstSLI GKGCMC 1 cut(s) 2197
BstSNI TACGTA 1 cut(s) 2141
BstUI CGCG 4 cut(s) 36, 318, 807, 1215
BstV2I GAAGAC 1 cut(s) 574
BstX2I RGATCY 9 cut(s) 540, 1291, 2170, 2822, 3148, 3917, 4147, 4468, 5071
BstXI CCANNNNNNTGG 1 cut(s) 4816
BstYI RGATCY 9 cut(s) 540, 1291, 2170, 2822, 3148, 3917, 4147, 4468, 5071
BstZI CGGCCG 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 4434
Bsu36I CCTNAGG 1 cut(s) 2178
BsuI GTATCC 1 cut(s) 2312
BsuTUI ATCGAT 1 cut(s) 4434
BtgI CCRYGG 4 cut(s) 316, 3570, 3952, 4386
BtgZI GCGATG 1 cut(s) 4626
BtrI CACGTC 1 cut(s) 4088
BtsI GCAGTG 3 cut(s) 1803, 4362, 4716
CaiI CAGNNNCTG 5 cut(s) 1516, 1649, 2048, 2414, 4361
CfoI GCGC 7 cut(s) 38, 93, 287, 1099, 2795, 3190, 3794
Cfr10I RCCGGY 1 cut(s) 38
Cfr42I CCGCGG 1 cut(s) 319
ClaI ATCGAT 1 cut(s) 4434
CseI GACGC 5 cut(s) 215, 338, 813, 2684, 3302
DraI TTTAAA 2 cut(s) 1678, 4335
DrdI GACNNNNNNGTC 1 cut(s) 1316
DriI GACNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 4 cut(s) 40, 319, 2439, 2484
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 7 cut(s) 203, 302, 317, 359, 2248, 4999, 5075
Eam1105I GACNNNNNGTC 1 cut(s) 211
EarI CTCTTC 7 cut(s) 203, 302, 317, 359, 2248, 4999, 5075
EciI GGCGGA 4 cut(s) 103, 115, 238, 4148
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco105I TACGTA 1 cut(s) 2141
Eco147I AGGCCT 2 cut(s) 2465, 3744
Eco24I GRGCYC 3 cut(s) 273, 414, 683
Eco31I GGTCTC 6 cut(s) 115, 314, 1288, 3228, 3560, 4185
Eco32I GATATC 2 cut(s) 4200, 4432
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
Eco57I CTGAAG 7 cut(s) 1016, 1814, 2352, 2708, 2949, 4104, 4113
Eco81I CCTNAGG 1 cut(s) 2178
Eco88I CYCGRG 1 cut(s) 1658
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 4 cut(s) 737, 1019, 2474, 4238
EcoRI GAATTC 1 cut(s) 2026
EcoRII CCWGG 9 cut(s) 86, 623, 770, 1390, 1957, 2292, 4360, 4405, 4973
EcoRV GATATC 2 cut(s) 4200, 4432
EcoT22I ATGCAT 2 cut(s) 1878, 2608
EcoT38I GRGCYC 3 cut(s) 273, 414, 683
Esp3I CGTCTC 1 cut(s) 4996
FalI AAGNNNNNCTT 4 cut(s) 3729, 3761, 4818, 4850
FaqI GGGAC 4 cut(s) 485, 544, 4066, 4212
FauI CCCGC 5 cut(s) 17, 280, 772, 1801, 1935
FauNDI CATATG 2 cut(s) 2935, 4501
FbaI TGATCA 4 cut(s) 952, 2203, 3838, 4447
FblI GTMKAC 1 cut(s) 243
FriOI GRGCYC 3 cut(s) 273, 414, 683
GlaI GCGC 7 cut(s) 37, 92, 286, 1098, 2794, 3189, 3793
GsaI CCCAGC 1 cut(s) 2473
GsuI CTGGAG 9 cut(s) 607, 843, 1109, 1217, 1374, 1410, 1755, 1980, 2964
HapII CCGG 3 cut(s) 39, 1447, 3469
HgaI GACGC 5 cut(s) 215, 338, 813, 2684, 3302
HhaI GCGC 7 cut(s) 38, 93, 287, 1099, 2795, 3190, 3794
Hin1I GRCGYC 2 cut(s) 207, 330
Hin6I GCGC 7 cut(s) 36, 91, 285, 1097, 2793, 3188, 3792
HinP1I GCGC 7 cut(s) 36, 91, 285, 1097, 2793, 3188, 3792
HincII GTYRAC 5 cut(s) 244, 1321, 1369, 1474, 2674
HindII GTYRAC 5 cut(s) 244, 1321, 1369, 1474, 2674
HindIII AAGCTT 1 cut(s) 3070
HpaI GTTAAC 1 cut(s) 1474
HpaII CCGG 3 cut(s) 39, 1447, 3469
Hpy99I CGWCG 7 cut(s) 191, 209, 245, 269, 332, 401, 1671
HpyCH4IV ACGT 6 cut(s) 612, 1418, 1666, 2140, 4087, 5043
HpySE526I ACGT 6 cut(s) 612, 1418, 1666, 2140, 4087, 5043
Hsp92I GRCGYC 2 cut(s) 207, 330
HspAI GCGC 7 cut(s) 36, 91, 285, 1097, 2793, 3188, 3792
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 4 cut(s) 952, 2203, 3838, 4447
KspAI GTTAAC 1 cut(s) 1474
KspI CCGCGG 1 cut(s) 319
LguI GCTCTTC 1 cut(s) 302
LweI GCATC 9 cut(s) 592, 1065, 1636, 2080, 2593, 2779, 2794, 3216, 3635
MaeII ACGT 6 cut(s) 612, 1418, 1666, 2140, 4087, 5043
MfeI CAATTG 2 cut(s) 1580, 3519
MflI RGATCY 9 cut(s) 540, 1291, 2170, 2822, 3148, 3917, 4147, 4468, 5071
MhlI GDGCHC 6 cut(s) 273, 414, 683, 2197, 2742, 5091
MlsI TGGCCA 1 cut(s) 2441
MluI ACGCGT 1 cut(s) 1213
MluNI TGGCCA 1 cut(s) 2441
MmeI TCCRAC 5 cut(s) 177, 966, 2578, 3167, 4668
Mox20I TGGCCA 1 cut(s) 2441
Mph1103I ATGCAT 2 cut(s) 1878, 2608
MroNI GCCGGC 1 cut(s) 38
MscI TGGCCA 1 cut(s) 2441
MslI CAYNNNNRTG 3 cut(s) 590, 1881, 2618
Msp20I TGGCCA 1 cut(s) 2441
MspA1I CMGCKG 5 cut(s) 318, 797, 2906, 4124, 4598
MspI CCGG 3 cut(s) 39, 1447, 3469
MunI CAATTG 2 cut(s) 1580, 3519
Mva1269I GAATGC 3 cut(s) 287, 2489, 2592
MvaI CCWGG 9 cut(s) 88, 625, 772, 1392, 1959, 2294, 4362, 4407, 4975
MvnI CGCG 4 cut(s) 36, 318, 807, 1215
NaeI GCCGGC 1 cut(s) 40
NciI CCSGG 1 cut(s) 3470
NcoI CCATGG 3 cut(s) 3570, 3952, 4386
NdeI CATATG 2 cut(s) 2935, 4501
NgoMIV GCCGGC 1 cut(s) 38
NmeAIII GCCGAG 3 cut(s) 30, 235, 372
NmuCI GTSAC 7 cut(s) 613, 933, 1550, 1832, 2428, 2497, 2611
NsiI ATGCAT 2 cut(s) 1878, 2608
NspI RCATGY 4 cut(s) 1880, 2561, 2754, 5114
NspV TTCGAA 2 cut(s) 1356, 3005
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 1880
PaeR7I CTCGAG 1 cut(s) 1658
PceI AGGCCT 2 cut(s) 2465, 3744
PciI ACATGT 1 cut(s) 5110
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 8 cut(s) 138, 213, 261, 405, 414, 1212, 2146, 4073
PctI GAATGC 3 cut(s) 287, 2489, 2592
PdiI GCCGGC 1 cut(s) 40
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 1403
Ple19I CGATCG 1 cut(s) 408
Ppu21I YACGTR 1 cut(s) 2141
PpuMI RGGWCCY 4 cut(s) 737, 1019, 2474, 4238
PscI ACATGT 1 cut(s) 5110
PshAI GACNNNNGTC 1 cut(s) 3954
PshBI ATTAAT 3 cut(s) 2991, 3434, 4265
PsiI TTATAA 2 cut(s) 2366, 3195
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp1406I AACGTT 1 cut(s) 1418
Psp5II RGGWCCY 4 cut(s) 737, 1019, 2474, 4238
Psp6I CCWGG 9 cut(s) 86, 623, 770, 1390, 1957, 2292, 4360, 4405, 4973
PspFI CCCAGC 1 cut(s) 2469
PspGI CCWGG 9 cut(s) 86, 623, 770, 1390, 1957, 2292, 4360, 4405, 4973
PspPPI RGGWCCY 4 cut(s) 737, 1019, 2474, 4238
PstI CTGCAG 5 cut(s) 1692, 2653, 4252, 4357, 4680
PstNI CAGNNNCTG 5 cut(s) 1516, 1649, 2048, 2414, 4361
PsuI RGATCY 9 cut(s) 540, 1291, 2170, 2822, 3148, 3917, 4147, 4468, 5071
PsyI GACNNNGTC 1 cut(s) 371
PvuI CGATCG 1 cut(s) 408
PvuII CAGCTG 3 cut(s) 2906, 4124, 4598
RseI CAYNNNNRTG 3 cut(s) 590, 1881, 2618
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 1 cut(s) 319
SalI GTCGAC 1 cut(s) 242
SapI GCTCTTC 1 cut(s) 302
ScaI AGTACT 1 cut(s) 944
SduI GDGCHC 6 cut(s) 273, 414, 683, 2197, 2742, 5091
SfaNI GCATC 9 cut(s) 592, 1065, 1636, 2080, 2593, 2779, 2794, 3216, 3635
SfcI CTRYAG 6 cut(s) 1688, 2649, 3417, 4248, 4353, 4676
Sfr274I CTCGAG 1 cut(s) 1658
Sfr303I CCGCGG 1 cut(s) 319
SfuI TTCGAA 2 cut(s) 1356, 3005
SgrBI CCGCGG 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 1658
SmiMI CAYNNNNRTG 3 cut(s) 590, 1881, 2618
SmlI CTYRAG 5 cut(s) 422, 1220, 1658, 4378, 4472
SmoI CTYRAG 5 cut(s) 422, 1220, 1658, 4378, 4472
SnaBI TACGTA 1 cut(s) 2141
SpeI ACTAGT 2 cut(s) 2036, 2945
SphI GCATGC 1 cut(s) 1880
SseBI AGGCCT 2 cut(s) 2465, 3744
SspI AATATT 2 cut(s) 978, 3294
SstI GAGCTC 2 cut(s) 414, 683
StuI AGGCCT 2 cut(s) 2465, 3744
TaiI ACGT 6 cut(s) 615, 1421, 1669, 2143, 4090, 5046
TaqII GACCGA 4 cut(s) 296, 622, 1521, 1995
TatI WGTACW 6 cut(s) 942, 1624, 3238, 4100, 4569, 4877
TseFI GTSAC 7 cut(s) 613, 933, 1550, 1832, 2428, 2497, 2611
Tsp45I GTSAC 7 cut(s) 613, 933, 1550, 1832, 2428, 2497, 2611
TspGWI ACGGA 3 cut(s) 205, 361, 2102
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 1403
VneI GTGCAC 1 cut(s) 2193
VspI ATTAAT 3 cut(s) 2991, 3434, 4265
XceI RCATGY 4 cut(s) 1880, 2561, 2754, 5114
XcmI CCANNNNNNNNNTGG 2 cut(s) 556, 4926
XhoI CTCGAG 1 cut(s) 1658
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 243
ZrmI AGTACT 1 cut(s) 944
Zsp2I ATGCAT 2 cut(s) 1878, 2608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.