MD10G1143300.v1.1

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002933
Physical Location & Seq
Forward (+)
22992106 .. 23001210
9105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1143300.v1.1.491

Sequence Viewer

Length: 5148 bp
ATGTCCGCTTCCCAAACCTTAGGCGGCCCCTCCCGCGCCGGCCGCCTGCTCGGCCCCTCCCTCGACAAAATCATCAAGAACGCCGCCTGGCGCAAGCACTCCCATTTGGTCTCCGCCTGCAAATCCGCCCTCGACAAGCTCGACTCCGTCACTGACTCCTCCATCATCGATCCCAAGTCCCCCGTCTCCGGCATCTCCCTCGCCGACGCCGAGTTCGCCCTCCGCCCCGTCCTCCTCGCCCTAGATTCGGCTTACCCCAAGGTCGTTGAGCCCGCCATCGACTGCGTCTTCAAGCTTTTCTCCCTTGGTATCTTCCGCGGTGAGATCGACACTTCCGATCCCAAATCTGTGCTTTTCAAGCTCGTCGAGTCGGTGTGTAAGTGCGCGGCGATCAGCGAGGAGCCTATCGAGCTCGGCGTGCTTCGGGTATTGCTCGCCGCCGTGAGATCGCCGCGGGTTTTGATACGCGGTGATTGTTTGGTGAATATTGTTAGGACTTGTTACAATGTGTACCTTGGTGGAGTGAATGGGACCAATCAGATCTGCGCCAAGTCGGTTTTGGCTCAGATTATGGTGATTGTTTTCACCAGAGTAGAGGAGGATTCGGTGAATGTCAGCATTTCTAGGGTTTCGGTGAACGAGTTGTTGGAATTCACTGATAAGAATTTGAACGAAGGGAGCTCCATTTTGTTGTGCCAGAATTTCATCAATGAGGTTATGGATGCGAGCTACGGCGGTCCGGATGGAATCAAAACGGTTGCCTCCCCAGGTCCCAAATTACAAAACGGCAATGCCAGTGGTGAGTCTAATAATGATGGAGCTGAATCCGGCGACTGTGCTAGTAAGATTAGGGATGATGGGTATCTTTTGTTTAAGAATTTGTGTAAGTTGTCCATGAAATACTCGTCACAGGAGCACTCTGATGATCAAATTCTCTTGAGGGGTAAAGTATTGTCCTTAGAGCTTTTGAAGGTGGTCATGGACAATGGGGGTCCAATTTGGCGCAACAATGAGAGGTTTCTCAATGCTGTCAAGCAGTTCCTCTGCTTATCACTGTTAAAAAACAGTGCGTTGTCAGTCATGGCCATTTTCCAGCTTCAGTGTTCTATTTTCACCAGCTTGTTATCGAAATTCAGATCAGGATTGAAAGCAGAAATTGGTATATTTTTTCCGATGCTTGTCCTACGCGTGCTTGAGAATGTTCTCCAGCCTAGTTTCTTGCAAAAAATGACAGTCCTCAATCTATTGGAGAAGATCTCTCAGGATTCGCAGATTATCATTGACATTTTTGTCAACTATGACTGTGATGTCGATGCCCCAAACATCTTTGAAAGGATTGTCAATGGCCTTCTGAAAACTGCTCTAGGACCTCCCACTGGTTCAACAACAACATTGTCTCCAGTCCAAGATATCACTTTCCGGCATGAATCCGTAAAGTGCTTGGTTAGCATCATAAATTCAATGGGATCTTGGATGGACCAACAGCTGAGTATGGGAGACTCCTATCTACCGAAGACCAATGAAAGCGACACCTCAGCTGAGAAAACAGAAAATAGTTCGACCCCAAATGGTGAAGAAGGAGCTGCTTTTGATAATGAAGTACATCCAGAAGGAAGTGCTGAAGTTTCAGATGCTGCAACTCTTGAGCAACGTCGAGCTTATAAGCTTGAACTTCAGAAAGGTGTCTCGCTGTTTAATAGGAAGCCTAACAAGGGCATTGAGTTTCTGATAAGCAGCAAAAAAGTTGGTAGTTCCCCAGAAGATGTGGCTTCTTTCCTGAGGAACAACACTGCTGGCCTAAATGAAACCATGATTGGTGATTATTTGGGTGAAAGGGAGGAATTTCCTCTAAAAGTTATGCATGCTTATGTCGATTCCTTTAATTTCAAAGGGATGGATTTCGGTGAAGCAATAAGGTTTTTCCTGCGGGGCTTCAGGTTACCTGGAGAAGCACAGAAAATTGACCGCATCATGGAGAAATTTGCTGAGCGCTATTGTAAATGCAGTCCAAATTCGTTTACAAGTGCAGATACTGCATATGTCCTTGCATACTCTGTGATAATGCTCAATACAGATGCCCATAACAACATGGTGAAAGATAAGATGACCAAGGCTGACTTCATCCGGAACAACCGAGGAATAGATGATGGAAAGGATCTACCTGAGGAGTATCTTGGTGTCCTATATGATCAAATTGTTAAAAATGAGATTAAGATGAGCGCCGATTCTTCTGTACCACAGAGCAAGCAGGAAAACAGTTTTAATAAATTATTAGGCTTGGATGGTATCCTGAATTTGGTAACTGGGAAGCAGACTGAAGAAAAAGCATTGGGTGCAAATGGTCTTCTTATAAAGCACATTCAAGAGCAATTTAAAGCAAAGTCAGGAAAATCAGAGTCTATCTATCATGCTGTGACAGATGTGGCAATTTTGAGGTTTATGGTGGAGGTCTGCTGGGGTCCTATGTTGGCTGCATTTAGTGTGACTCTTGACCAAAGTGATGACAGGCTTGCTACTTCTCAATGCTTACAAGGTTTTCGGCATGCTGTTCATGTTACTGCTTTGATGGGAATGCAGACCCAGAGAGATGCATTTGTCACTTCAGTAGCAAAGTTTACTTATCTACATAATGCTGCAGATATGAGGCAAAAAAATGTTGATGCTGTGAAGGCAATAATATCAATTGCCATTGAAGATGGTAATCATCTTCAGGAAGCATGGGAACACATATTAACGTGTCTTTCCCGAATCGAGCATTTGCAACTGTTGGGAGAAGGTGCACCCACTGATGCATCCTTTTTTACTGGATCAAAGGTCGAAACAGAGGAAAAATCACCAAAGCCTACTGGACTTTCTTCTCTGACGAAAAAAGGAACTATACAGAATCCAGCTGTGATGGCTGTTGTTCGTGGGGGTTCGTATGACAGTACTAGTGTCCGGGTGAACACTTCTGGACTGGTAACTCCAGAACAGATTAATAACTTCATTTCAAACTTGAATTTGCTGGATCAGATTGGGAATTTTGAGTTGAATCATGTATTTGCTCATAGCCAAAGATTAAACAGTGAAGCAATAGTGGCTTTTGTGAAGGCCTTGTGCAAAGTTTCCATGGCAGAGTTGCAATCTCCAACAGATCCTCGCGTATTCAGCCTCACAAAAATAGTTGAAATAGCGCATTACAATATGAACCGCATCAGATTAGTGTGGTCTCGCATATGGAATGTTCTCTCAGATTTCTTTGTATCAGTTGGCTTGTCAGAGAACCTCTCAGTTGCAATATTTGTGATGGATTCATTACGTCAGCTTGCTATGAAATTCTTAGAGCGCGAGGAGCTGGCAAATTACAACTTCCAGAATGAATTTTTGAGACCATTTGTGATTGTTATGCAAAAAAGCAACTCTACAGAAATTAGGGAATTAATTGTTCGGTGCATTTCACAAATGGTCCTCAGCCGTGTCAATCATGTGAAATCTGGCTGGAAAAGTGTATTTCTGGTTTTTACAGCAGCGGCAGCTGATGAGCGGAAAAATATTGTCTTGTTGGCCTTTGAGACCATGGAAAAAATCGTCAGAGAATACTTCCCCTATATAACAGAGACAGAGACAATGACTTTTACTGATTGTGTTAGATGCCTCTTAACCTTCACGAATAGCAGATTCAATAGTGATGTTAGCCTCAACGCTATTGCATTTCTCCGGTACTGTGCTGTCAAACTTGCTGAAGGAGGGCTTGTTTACAACAAGAGGAGTGAGCTTGATGTTTCATCCCTTCCAACAGCGAATGAGGATGCTTCAAATGGAGTGACTTTCAATGAGAAAGATGAACATGCATCCTTTTGGGTTCCATTGCTAACAGGTTTGTCAAAGCTAACATCAGATCCTAGATCAGCAATCAGGAAGGGTTCTTTGGAAGTTCTTTTCAACATTCTAAAGGATCATGGTCATCTCTTCTCACGCTCCTTTTGGACTGCGATATTCAATTCTTTTGTTTACCCCATATTTAGCTGTGTATGCGGTAAGAAAGACACACATATGGAAAAAGACCAGTCTTCACCAGTTTCAGTATCTCCGCGTCCTGATGGAAGCACATGGGATTCTGAAACTTCTGCAGTTGCAGCAGACTGTTTCATAGATCTATTTGTCAGTTTTTTCGATACAGTAAGGCCGCAACTACCAGGTGTGGTATCCATTCTGACAGGACTCATACGAAGTCCTGTTCAGGGTCCCGCTAGCACTGGGGTTGCAGGTTTAGTGCGTTTGGCTGGTGAAGTTGGTGACAAGCTTTCAGAAGATGAATGGAGAGAGATCTTTCTGGCTTTAAAAGAAGCAACCACATCTTCCGTGCCTGGATTTATGAAGGTGTTAAGAACCATGGACGACATCAATATTCCCGGTCTTTCTCAATCTTATAGCGATATAGATTTGTCATCTGATCATGGGTTTACAAATGATGATCTTGAGGATGATAATCTGCAGACAGCATCATATTTGGTATCAAGAATGAAGAGTCATATTACTATGCAGCTACTTATCATACAGGTTGCTACTGATTTGTACAAGTTACACCTTGAATCGTTATCAGTTGGCAACATTTCAATCCTCCTTGAAATATTTTCCCTCATTGCATCCCATGCTCACCAACTGAACTCAGAGACAATTCTGCACAAGAAGCTGCAAAAAGTGTGCTCCGTCCTGGAACTCACTTCCCCACCTCTGGTTCATTTCGAGAATGATTCTTACAAGAACTACTTAAGCTTCCTCCAAAACGCCCTCGTGGACAATCCTTCTCTGTCGAAGGAGATGAACATAGAAGCCAAACTCGTTGGAGTGTGTGAAAGTATATTGCAGATATACCTAAAGTGTACCGAGCTTCATTCTGCCGAGCAGAGGCCTGCCGATCAGCCAGTTTTGCACTGGATTCTTCCCTTGGGCACAGCAAAGAAGGAAGAATTGGCCGCGAGGACCGACATAGCCGTGTCAGCGTTGCAGGTATTGAACAGTCTGGAAAAGGTTTCGTTTAGGAGGCACGTCTCTCGGTTATTTCCGTTGTTAGCAGATCTTGTGAGGAGTGAGCACACATCTGGAGAAGTTCAGCTTGTTCTAAGCAACATATTCCAATCATGTATAGGCCCAATGGTAATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1716

Amino Acids

189.76

Weight (kDa)

5.67

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 20 - 201 2.1e-23 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 284 - 442 8e-43 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 555 - 737 4.7e-75 Sec7 domain
Sec7-like_HDS PF09324 1105 - 1187 1.3e-29 Mon2/Sec7/BIG1-like, HDS
BIG2_C PF20252 1507 - 1706 1.8e-30 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1662, 2351
AasI GACNNNNNNGTC 2 cut(s) 1289, 1307
Acc36I ACCTGC 2 cut(s) 4235, 4978
AccBSI CCGCTC 1 cut(s) 3523
AccIII TCCGGA 2 cut(s) 739, 2124
AclWI GGATC 9 cut(s) 164, 332, 1474, 2163, 2815, 3015, 3128, 3872, 3942
AcoI YGGCCR 3 cut(s) 40, 1083, 4953
AcuI CTGAAG 8 cut(s) 1082, 1641, 1658, 1918, 2337, 2586, 2693, 3741
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 7 cut(s) 512, 1602, 2235, 2929, 3701, 4556, 4864
AfeI AGCGCT 1 cut(s) 1991
AfiI CCNNNNNNNGG 9 cut(s) 188, 247, 1376, 1712, 1972, 2296, 4220, 4714, 4887
AflII CTTAAG 1 cut(s) 4750
AflIII ACRYGT 2 cut(s) 1186, 2735
AhlI ACTAGT 1 cut(s) 2930
AjiI CACGTC 1 cut(s) 5029
AjnI CCWGG 6 cut(s) 86, 766, 1942, 4174, 4345, 4692
Alw21I GWGCWC 6 cut(s) 414, 683, 918, 2782, 4688, 5076
Alw44I GTGCAC 1 cut(s) 2778
AlwI GGATC 9 cut(s) 164, 332, 1474, 2163, 2815, 3015, 3128, 3872, 3942
AlwNI CAGNNNCTG 2 cut(s) 1634, 2033
Aor13HI TCCGGA 2 cut(s) 739, 2124
Aor51HI AGCGCT 1 cut(s) 1991
ApaLI GTGCAC 1 cut(s) 2778
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AseI ATTAAT 2 cut(s) 2976, 3419
AspLEI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 1992, 2222, 3175, 3327
AsuC2I CCSGG 2 cut(s) 2939, 4392
AsuNHI GCTAGC 1 cut(s) 4229
AxyI CCTNAGG 3 cut(s) 19, 1778, 2163
BaeGI GKGCMC 2 cut(s) 2782, 4934
BalI TGGCCA 1 cut(s) 1085
BanII GRGCYC 3 cut(s) 273, 414, 683
BauI CACGAG 1 cut(s) 4772
BbsI GAAGAC 4 cut(s) 280, 1520, 2336, 4041
Bbv12I GWGCWC 6 cut(s) 414, 683, 918, 2782, 4688, 5076
BbvCI CCTCAGC 2 cut(s) 1534, 3449
BceAI ACGGC 5 cut(s) 425, 748, 802, 3438, 4958
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 5015, 5049
BciT130I CCWGG 6 cut(s) 88, 768, 1944, 4176, 4347, 4694
BciVI GTATCC 2 cut(s) 2297, 4195
BclI TGATCA 3 cut(s) 925, 2188, 4432
BcnI CCSGG 2 cut(s) 2939, 4392
BcuI ACTAGT 1 cut(s) 2930
BfaI CTAG 8 cut(s) 242, 624, 840, 1212, 1364, 2931, 3882, 4230
BfmI CTRYAG 4 cut(s) 2634, 3402, 4107, 4472
BfoI RGCGCY 2 cut(s) 1993, 2223
BfrI CTTAAG 1 cut(s) 4750
BfuAI ACCTGC 2 cut(s) 4235, 4978
BfuI GTATCC 2 cut(s) 2297, 4195
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 5 cut(s) 540, 1254, 4132, 4305, 5056
BlpI GCTNAGC 1 cut(s) 1986
BmcAI AGTACT 1 cut(s) 2929
Bme1390I CCNGG 8 cut(s) 88, 768, 1944, 2939, 4176, 4347, 4392, 4694
BmgBI CACGTC 1 cut(s) 5029
BmrFI CCNGG 8 cut(s) 88, 768, 1944, 2939, 4176, 4347, 4392, 4694
BmrI ACTGGG 2 cut(s) 2313, 4245
BmtI GCTAGC 1 cut(s) 4233
BmuI ACTGGG 2 cut(s) 2313, 4245
BpiI GAAGAC 4 cut(s) 280, 1520, 2336, 4041
BplI GAGNNNNNCTC 4 cut(s) 1241, 1273, 3251, 3283
BpmI CTGGAG 5 cut(s) 1190, 1383, 1965, 2949, 5103
Bpu10I CCTNAGC 2 cut(s) 1534, 3449
Bpu1102I GCTNAGC 1 cut(s) 1986
BpuEI CTTGAG 4 cut(s) 958, 1214, 1664, 4478
BpuMI CCSGG 2 cut(s) 2939, 4392
Bsa29I ATCGAT 1 cut(s) 168
BsaBI GATNNNNATC 3 cut(s) 861, 2700, 4467
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 4 cut(s) 115, 3213, 3361, 3545
BsaWI WCCGGW 3 cut(s) 739, 2124, 3696
BsaXI ACNNNNNCTCC 4 cut(s) 1381, 1411, 3717, 3747
Bsc4I CCNNNNNNNGG 9 cut(s) 188, 247, 1376, 1712, 1972, 2296, 4220, 4714, 4887
Bse118I RCCGGY 1 cut(s) 38
Bse21I CCTNAGG 3 cut(s) 19, 1778, 2163
Bse3DI GCAATG 3 cut(s) 796, 3845, 4620
Bse8I GATNNNNATC 3 cut(s) 861, 2700, 4467
BseAI TCCGGA 2 cut(s) 739, 2124
BseBI CCWGG 6 cut(s) 88, 768, 1944, 4176, 4347, 4694
BseCI ATCGAT 1 cut(s) 168
BseJI GATNNNNATC 3 cut(s) 861, 2700, 4467
BseLI CCNNNNNNNGG 9 cut(s) 188, 247, 1376, 1712, 1972, 2296, 4220, 4714, 4887
BseMI GCAATG 3 cut(s) 796, 3845, 4620
BseRI GAGGAG 8 cut(s) 148, 224, 413, 611, 2180, 3344, 3760, 5080
BseSI GKGCMC 2 cut(s) 2782, 4934
BseX3I CGGCCG 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 2454
BsgI GTGCAG 2 cut(s) 2046, 4646
Bsh1285I CGRYCG 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 6 cut(s) 414, 683, 918, 2782, 4688, 5076
BsiSI CCGG 9 cut(s) 39, 189, 740, 828, 1420, 2125, 2938, 3697, 4392
BslFI GGGAC 4 cut(s) 163, 544, 756, 4209
BslI CCNNNNNNNGG 9 cut(s) 188, 247, 1376, 1712, 1972, 2296, 4220, 4714, 4887
BsmBI CGTCTC 2 cut(s) 190, 5035
BsmFI GGGAC 4 cut(s) 163, 544, 756, 4209
BsmI GAATGC 1 cut(s) 2577
Bso31I GGTCTC 4 cut(s) 115, 3213, 3361, 3545
Bsp1286I GDGCHC 8 cut(s) 273, 414, 683, 918, 2782, 4688, 4934, 5076
Bsp13I TCCGGA 2 cut(s) 739, 2124
Bsp1407I TGTACA 1 cut(s) 4554
Bsp1720I GCTNAGC 1 cut(s) 1986
Bsp19I CCATGG 3 cut(s) 3108, 3555, 4371
BspDI ATCGAT 1 cut(s) 168
BspEI TCCGGA 2 cut(s) 739, 2124
BspMAI CTGCAG 3 cut(s) 2638, 4111, 4476
BspMI ACCTGC 2 cut(s) 4235, 4978
BspOI GCTAGC 1 cut(s) 4233
BspPI GGATC 9 cut(s) 164, 332, 1474, 2163, 2815, 3015, 3128, 3872, 3942
BspTI CTTAAG 1 cut(s) 4750
BspTNI GGTCTC 4 cut(s) 115, 3213, 3361, 3545
BsrBI CCGCTC 1 cut(s) 3523
BsrDI GCAATG 3 cut(s) 796, 3845, 4620
BsrFI RCCGGY 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 4554
BssAI RCCGGY 1 cut(s) 38
BssNI GRCGYC 1 cut(s) 207
BssSI CACGAG 1 cut(s) 4772
BssT1I CCWWGG 8 cut(s) 258, 304, 514, 2109, 3108, 3555, 4371, 4926
Bst2BI CACGAG 1 cut(s) 4772
Bst2UI CCWGG 6 cut(s) 88, 768, 1944, 4176, 4347, 4694
Bst6I CTCTTC 2 cut(s) 3953, 4499
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 4750
BstAPI GCANNNNNTGC 3 cut(s) 2033, 2333, 4631
BstAUI TGTACA 1 cut(s) 4554
BstDSI CCRYGG 5 cut(s) 316, 452, 3108, 3555, 4371
BstEII GGTNACC 1 cut(s) 1938
BstH2I RGCGCY 2 cut(s) 1993, 2223
BstHHI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 1992, 2222, 3175, 3327
BstMCI CGRYCG 1 cut(s) 43
BstNI CCWGG 6 cut(s) 88, 768, 1944, 4176, 4347, 4694
BstNSI RCATGY 3 cut(s) 1865, 2546, 3830
BstPI GGTNACC 1 cut(s) 1938
BstSCI CCNGG 8 cut(s) 86, 766, 1942, 2937, 4174, 4345, 4390, 4692
BstSFI CTRYAG 4 cut(s) 2634, 3402, 4107, 4472
BstSLI GKGCMC 2 cut(s) 2782, 4934
BstV2I GAAGAC 4 cut(s) 280, 1520, 2336, 4041
BstX2I RGATCY 9 cut(s) 540, 1254, 1466, 2155, 3133, 3877, 4132, 4305, 5056
BstYI RGATCY 9 cut(s) 540, 1254, 1466, 2155, 3133, 3877, 4132, 4305, 5056
BstZI CGGCCG 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 3 cut(s) 19, 1778, 2163
BsuI GTATCC 2 cut(s) 2297, 4195
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 5 cut(s) 316, 452, 3108, 3555, 4371
BtrI CACGTC 1 cut(s) 5029
BtsI GCAGTG 1 cut(s) 1788
BveI ACCTGC 2 cut(s) 4235, 4978
CaiI CAGNNNCTG 2 cut(s) 1634, 2033
CfoI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 1992, 2222, 3175, 3327
Cfr10I RCCGGY 1 cut(s) 38
Cfr42I CCGCGG 2 cut(s) 319, 455
ClaI ATCGAT 1 cut(s) 168
CpoI CGGWCCG 1 cut(s) 737
CseI GACGC 3 cut(s) 215, 274, 4061
CsiI ACCWGGT 1 cut(s) 4174
Csp6I GTAC 7 cut(s) 511, 1601, 2234, 2928, 3700, 4555, 4863
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CspI CGGWCCG 1 cut(s) 737
CviQI GTAC 7 cut(s) 511, 1601, 2234, 2928, 3700, 4555, 4863
DraI TTTAAA 2 cut(s) 2374, 4320
DrdI GACNNNNNNGTC 2 cut(s) 1289, 1307
DseDI GACNNNNNNGTC 2 cut(s) 1289, 1307
EaeI YGGCCR 3 cut(s) 40, 1083, 4953
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 3953, 4499
EarI CTCTTC 2 cut(s) 3953, 4499
EciI GGCGGA 3 cut(s) 103, 115, 212
Ecl136II GAGCTC 2 cut(s) 412, 681
EclXI CGGCCG 1 cut(s) 40
Eco130I CCWWGG 8 cut(s) 258, 304, 514, 2109, 3108, 3555, 4371, 4926
Eco147I AGGCCT 2 cut(s) 3092, 4891
Eco24I GRGCYC 3 cut(s) 273, 414, 683
Eco31I GGTCTC 4 cut(s) 115, 3213, 3361, 3545
Eco32I GATATC 1 cut(s) 1411
Eco47III AGCGCT 1 cut(s) 1991
Eco52I CGGCCG 1 cut(s) 40
Eco53kI GAGCTC 2 cut(s) 412, 681
Eco57I CTGAAG 8 cut(s) 1082, 1641, 1658, 1918, 2337, 2586, 2693, 3741
Eco81I CCTNAGG 3 cut(s) 19, 1778, 2163
Eco91I GGTNACC 1 cut(s) 1938
EcoICRI GAGCTC 2 cut(s) 412, 681
EcoO109I RGGNCCY 4 cut(s) 770, 1367, 2459, 4223
EcoO65I GGTNACC 1 cut(s) 1938
EcoRI GAATTC 1 cut(s) 650
EcoRII CCWGG 6 cut(s) 86, 766, 1942, 4174, 4345, 4692
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 8 cut(s) 258, 304, 514, 2109, 3108, 3555, 4371, 4926
EcoT22I ATGCAT 4 cut(s) 1863, 2593, 2794, 3832
EcoT38I GRGCYC 3 cut(s) 273, 414, 683
ErhI CCWWGG 8 cut(s) 258, 304, 514, 2109, 3108, 3555, 4371, 4926
Esp3I CGTCTC 2 cut(s) 190, 5035
FalI AAGNNNNNCTT 8 cut(s) 2102, 2134, 3714, 3746, 4733, 4765, 5079, 5111
FaqI GGGAC 4 cut(s) 163, 544, 756, 4209
FauI CCCGC 5 cut(s) 41, 280, 447, 1920, 4234
FauNDI CATATG 3 cut(s) 2038, 3215, 4032
FbaI TGATCA 3 cut(s) 925, 2188, 4432
FriOI GRGCYC 3 cut(s) 273, 414, 683
FspBI CTAG 8 cut(s) 242, 624, 840, 1212, 1364, 2931, 3882, 4230
GlaI GCGC 9 cut(s) 37, 92, 385, 547, 1004, 1991, 2221, 3174, 3326
GsaI CCCAGC 1 cut(s) 2458
GsuI CTGGAG 5 cut(s) 1190, 1383, 1965, 2949, 5103
HaeII RGCGCY 2 cut(s) 1993, 2223
HapII CCGG 9 cut(s) 39, 189, 740, 828, 1420, 2125, 2938, 3697, 4392
HgaI GACGC 3 cut(s) 215, 274, 4061
HhaI GCGC 9 cut(s) 38, 93, 386, 548, 1005, 1992, 2222, 3175, 3327
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 9 cut(s) 36, 91, 384, 546, 1003, 1990, 2220, 3173, 3325
HinP1I GCGC 9 cut(s) 36, 91, 384, 546, 1003, 1990, 2220, 3173, 3325
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HindIII AAGCTT 4 cut(s) 293, 1664, 4280, 4753
HpaII CCGG 9 cut(s) 39, 189, 740, 828, 1420, 2125, 2938, 3697, 4392
Hpy99I CGWCG 3 cut(s) 209, 368, 1656
HpyCH4IV ACGT 4 cut(s) 1651, 2735, 3298, 5028
HpySE526I ACGT 4 cut(s) 1651, 2735, 3298, 5028
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 9 cut(s) 36, 91, 384, 546, 1003, 1990, 2220, 3173, 3325
KflI GGGWCCC 1 cut(s) 4223
Kpn2I TCCGGA 2 cut(s) 739, 2124
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 3 cut(s) 925, 2188, 4432
KspI CCGCGG 2 cut(s) 319, 455
LmnI GCTCC 9 cut(s) 400, 678, 686, 818, 913, 1580, 3331, 3962, 4691
MabI ACCWGGT 1 cut(s) 4174
MaeI CTAG 8 cut(s) 242, 624, 840, 1212, 1364, 2931, 3882, 4230
MaeII ACGT 4 cut(s) 1651, 2735, 3298, 5028
MbiI CCGCTC 1 cut(s) 3523
MfeI CAATTG 1 cut(s) 2682
MflI RGATCY 9 cut(s) 540, 1254, 1466, 2155, 3133, 3877, 4132, 4305, 5056
MhlI GDGCHC 8 cut(s) 273, 414, 683, 918, 2782, 4688, 4934, 5076
MlsI TGGCCA 1 cut(s) 1085
MluI ACGCGT 1 cut(s) 1186
MluNI TGGCCA 1 cut(s) 1085
MlyI GAGTC 9 cut(s) 137, 149, 377, 812, 1493, 2405, 2479, 4194, 4516
MmeI TCCRAC 4 cut(s) 627, 3152, 3797, 4804
Mox20I TGGCCA 1 cut(s) 1085
Mph1103I ATGCAT 4 cut(s) 1863, 2593, 2794, 3832
MroI TCCGGA 2 cut(s) 739, 2124
MroNI GCCGGC 1 cut(s) 38
MscI TGGCCA 1 cut(s) 1085
MslI CAYNNNNRTG 4 cut(s) 921, 1866, 4031, 4973
Msp20I TGGCCA 1 cut(s) 1085
MspA1I CMGCKG 7 cut(s) 318, 454, 1486, 1538, 2891, 3509, 3515
MspCI CTTAAG 1 cut(s) 4750
MspI CCGG 9 cut(s) 39, 189, 740, 828, 1420, 2125, 2938, 3697, 4392
MspR9I CCNGG 8 cut(s) 88, 768, 1944, 2939, 4176, 4347, 4392, 4694
MunI CAATTG 1 cut(s) 2682
Mva1269I GAATGC 1 cut(s) 2577
MvaI CCWGG 6 cut(s) 88, 768, 1944, 4176, 4347, 4694
NaeI GCCGGC 1 cut(s) 40
NciI CCSGG 2 cut(s) 2939, 4392
NcoI CCATGG 3 cut(s) 3108, 3555, 4371
NdeI CATATG 3 cut(s) 2038, 3215, 4032
NgoMIV GCCGGC 1 cut(s) 38
NheI GCTAGC 1 cut(s) 4229
NmeAIII GCCGAG 4 cut(s) 30, 235, 393, 4906
NmuCI GTSAC 7 cut(s) 148, 906, 2413, 2482, 2596, 3802, 4274
NsiI ATGCAT 4 cut(s) 1863, 2593, 2794, 3832
NspI RCATGY 3 cut(s) 1865, 2546, 3830
PaeI GCATGC 2 cut(s) 1865, 2546
PceI AGGCCT 2 cut(s) 3092, 4891
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 207, 333, 414
PctI GAATGC 1 cut(s) 2577
PdiI GCCGGC 1 cut(s) 40
PflFI GACNNNGTC 2 cut(s) 146, 284
PfoI TCCNGGA 1 cut(s) 4692
PleI GAGTC 9 cut(s) 137, 149, 376, 811, 1493, 2404, 2479, 4194, 4515
PpsI GAGTC 9 cut(s) 137, 149, 376, 811, 1493, 2404, 2479, 4194, 4515
PpuMI RGGWCCY 4 cut(s) 770, 1367, 2459, 4223
PshBI ATTAAT 2 cut(s) 2976, 3419
PsiI TTATAA 2 cut(s) 1662, 2351
Psp124BI GAGCTC 2 cut(s) 414, 683
Psp5II RGGWCCY 4 cut(s) 770, 1367, 2459, 4223
Psp6I CCWGG 6 cut(s) 86, 766, 1942, 4174, 4345, 4692
PspEI GGTNACC 1 cut(s) 1938
PspFI CCCAGC 1 cut(s) 2454
PspGI CCWGG 6 cut(s) 86, 766, 1942, 4174, 4345, 4692
PspPPI RGGWCCY 4 cut(s) 770, 1367, 2459, 4223
PstI CTGCAG 3 cut(s) 2638, 4111, 4476
PstNI CAGNNNCTG 2 cut(s) 1634, 2033
PsuI RGATCY 9 cut(s) 540, 1254, 1466, 2155, 3133, 3877, 4132, 4305, 5056
PsyI GACNNNGTC 2 cut(s) 146, 284
PvuII CAGCTG 4 cut(s) 1486, 1538, 2891, 3515
RsaI GTAC 7 cut(s) 512, 1602, 2235, 2929, 3701, 4556, 4864
RsaNI GTAC 7 cut(s) 511, 1601, 2234, 2928, 3700, 4555, 4863
RseI CAYNNNNRTG 4 cut(s) 921, 1866, 4031, 4973
Rsr2I CGGWCCG 1 cut(s) 737
RsrII CGGWCCG 1 cut(s) 737
SacI GAGCTC 2 cut(s) 414, 683
SacII CCGCGG 2 cut(s) 319, 455
ScaI AGTACT 1 cut(s) 2929
SchI GAGTC 9 cut(s) 137, 149, 377, 812, 1493, 2405, 2479, 4194, 4516
ScrFI CCNGG 8 cut(s) 88, 768, 1944, 2939, 4176, 4347, 4392, 4694
SduI GDGCHC 8 cut(s) 273, 414, 683, 918, 2782, 4688, 4934, 5076
SexAI ACCWGGT 1 cut(s) 4174
SfcI CTRYAG 4 cut(s) 2634, 3402, 4107, 4472
Sfr303I CCGCGG 2 cut(s) 319, 455
SgrBI CCGCGG 2 cut(s) 319, 455
SmiMI CAYNNNNRTG 4 cut(s) 921, 1866, 4031, 4973
SmlI CTYRAG 5 cut(s) 937, 1193, 1643, 4457, 4750
SmoI CTYRAG 5 cut(s) 937, 1193, 1643, 4457, 4750
SpeI ACTAGT 1 cut(s) 2930
SphI GCATGC 2 cut(s) 1865, 2546
SseBI AGGCCT 2 cut(s) 3092, 4891
SspI AATATT 5 cut(s) 487, 3279, 3532, 4387, 4611
SspMI CTAG 8 cut(s) 242, 624, 840, 1212, 1364, 2931, 3882, 4230
SstI GAGCTC 2 cut(s) 414, 683
StuI AGGCCT 2 cut(s) 3092, 4891
StyD4I CCNGG 8 cut(s) 86, 766, 1942, 2937, 4174, 4345, 4390, 4692
StyI CCWWGG 8 cut(s) 258, 304, 514, 2109, 3108, 3555, 4371, 4926
TaiI ACGT 4 cut(s) 1654, 2738, 3301, 5031
TaqII GACCGA 1 cut(s) 4979
TatI WGTACW 3 cut(s) 1600, 2927, 4554
TauI GCSGC 9 cut(s) 27, 45, 86, 389, 440, 454, 3512, 4168, 4958
TseFI GTSAC 7 cut(s) 148, 906, 2413, 2482, 2596, 3802, 4274
Tsp45I GTSAC 7 cut(s) 148, 906, 2413, 2482, 2596, 3802, 4274
TspGWI ACGGA 5 cut(s) 136, 1420, 4330, 4678, 5034
Tth111I GACNNNGTC 2 cut(s) 146, 284
Vha464I CTTAAG 1 cut(s) 4750
VneI GTGCAC 1 cut(s) 2778
VspI ATTAAT 2 cut(s) 2976, 3419
XceI RCATGY 3 cut(s) 1865, 2546, 3830
XcmI CCANNNNNNNNNTGG 2 cut(s) 556, 4911
XspI CTAG 8 cut(s) 242, 624, 840, 1212, 1364, 2931, 3882, 4230
ZrmI AGTACT 1 cut(s) 2929
Zsp2I ATGCAT 4 cut(s) 1863, 2593, 2794, 3832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.