pycom10g12340

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002933
Physical Location & Seq
Forward (+)
15854954 .. 15856060
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12340.1

Sequence Viewer

Length: 714 bp
ATGGGTTTACAAATGATCGATCTTGAGGATGATAATCTGCAAACAGCATCATATTTGGTTTCAAGAATGAAGAGTCATATTGCTATGCAGCTACTTATCATACAGGTTGCTACTGATTTGTACAAGTTACACCTTGAATCGTTATCGGTTGGCAACATTTCAATCCTCCTTGAAATATTTTCCCTCATTGCATCCCATGCTCACCAACTGAACTCAGAGACAATTCTGCACAAGAAGCTGCAAAAAGTGTGCTCCGTCCTGGAACTCACTTCCCCACCTCTGGTTCATTTCGAGAATGATTCTTACAAGAACTACTTAAGCTTCCTCCAAAACGTGCTCGTGGACAATCCTTCTCTTTCGAAGGAGATGAACATTGAAGCCAAACTCGTTGGAGTGTGTGAAAGTATATTGCAGATATACCTGAAGTGTACCGGGCTTCATTCTGCCGAGCAGAGGCATGCTGATCAGCCGATTTTGCACTGGATTCTTCCCTTGGGCACGGCAAAGAAGGAAGAATTGGCCGCGAGGACCGACATAGCCGTGTCAGCGTTGCAGGTATTGAACAGTCTGGAAAAGGTTTCGTTTAGGAGGCACGTCTCTCGGTTATTTCCGTTGTTCGTAGATCTTGTGAGGAGTGAGCACGCATCTGGAGAAGTTCAGCTTGTTCTAAGCAACATATTCCAATCATGTATAGGCCCAATAATAATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032588 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

238

Amino Acids

26.6

Weight (kDa)

6.1

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BIG2_C PF20252 29 - 228 5.8e-33 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AccII CGCG 1 cut(s) 524
AciI CCGC 1 cut(s) 522
AcoI YGGCCR 1 cut(s) 519
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 2 cut(s) 122, 430
AfiI CCNNNNNNNGG 2 cut(s) 280, 453
AflII CTTAAG 1 cut(s) 316
AgsI TTSAA 6 cut(s) 63, 137, 162, 173, 377, 562
AjiI CACGTC 1 cut(s) 595
AjnI CCWGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 4 cut(s) 91, 238, 321, 661
AluI AGCT 4 cut(s) 91, 238, 321, 661
Alw21I GWGCWC 3 cut(s) 254, 339, 642
Alw26I GTCTC 2 cut(s) 212, 601
AoxI GGCC 2 cut(s) 519, 694
ApeKI GCWGC 2 cut(s) 88, 238
AspS9I GGNCC 2 cut(s) 528, 695
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 194
AsuII TTCGAA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 500
BauI CACGAG 1 cut(s) 338
Bbv12I GWGCWC 3 cut(s) 254, 339, 642
BbvI GCAGC 2 cut(s) 100, 225
BceAI ACGGC 2 cut(s) 516, 524
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 1 cut(s) 260
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 1 cut(s) 433
BcoDI GTCTC 2 cut(s) 212, 601
BfrI CTTAAG 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 544
BglII AGATCT 1 cut(s) 622
BisI GCNGC 3 cut(s) 89, 239, 522
BlsI GCNGC 3 cut(s) 90, 240, 523
Bme1390I CCNGG 2 cut(s) 260, 433
Bme18I GGWCC 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 595
BmgT120I GGNCC 2 cut(s) 528, 695
BmrFI CCNGG 2 cut(s) 260, 433
BmsI GCATC 3 cut(s) 56, 200, 653
BpmI CTGGAG 1 cut(s) 669
Bpu14I TTCGAA 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 433
Bsa29I ATCGAT 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 492
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 453
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 186
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 260
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 2 cut(s) 34, 191
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 2 cut(s) 280, 453
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 646
BseSI GKGCMC 1 cut(s) 500
BseXI GCAGC 2 cut(s) 100, 225
BsgI GTGCAG 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 2 cut(s) 521, 696
BshVI ATCGAT 1 cut(s) 18
BsiHKAI GWGCWC 3 cut(s) 254, 339, 642
BsiSI CCGG 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 280, 453
BsmAI GTCTC 2 cut(s) 212, 601
BsmBI CGTCTC 1 cut(s) 601
BsnI GGCC 2 cut(s) 521, 696
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 4 cut(s) 254, 339, 500, 642
Bsp1407I TGTACA 1 cut(s) 120
Bsp143I GATC 4 cut(s) 15, 19, 463, 622
BspACI CCGC 1 cut(s) 522
BspANI GGCC 2 cut(s) 521, 696
BspCNI CTCAG 1 cut(s) 227
BspDI ATCGAT 1 cut(s) 18
BspFNI CGCG 1 cut(s) 524
BspMI ACCTGC 1 cut(s) 544
BspT104I TTCGAA 1 cut(s) 359
BspTI CTTAAG 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 120
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 4 cut(s) 15, 19, 463, 622
BssSI CACGAG 1 cut(s) 338
BssT1I CCWWGG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 1 cut(s) 65
BstAFI CTTAAG 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 197
BstAUI TGTACA 1 cut(s) 120
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 459, 642
BstDEI CTNAG 2 cut(s) 214, 668
BstF5I GGATG 2 cut(s) 34, 191
BstFNI CGCG 1 cut(s) 524
BstKTI GATC 4 cut(s) 18, 22, 466, 625
BstMAI GTCTC 2 cut(s) 212, 601
BstMBI GATC 4 cut(s) 15, 19, 463, 622
BstMWI GCNNNNNNNGC 4 cut(s) 197, 235, 475, 545
BstNI CCWGG 1 cut(s) 260
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 258, 431
BstSLI GKGCMC 1 cut(s) 500
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 2 cut(s) 100, 225
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
Bsu15I ATCGAT 1 cut(s) 18
BsuRI GGCC 2 cut(s) 521, 696
BsuTUI ATCGAT 1 cut(s) 18
BtrI CACGTC 1 cut(s) 595
BtsCI GGATG 2 cut(s) 34, 191
BtsIMutI CAGTG 1 cut(s) 478
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 2 cut(s) 459, 642
Cfr13I GGNCC 2 cut(s) 528, 695
ClaI ATCGAT 1 cut(s) 18
Csp6I GTAC 2 cut(s) 121, 429
CviAII CATG 3 cut(s) 197, 458, 687
CviQI GTAC 2 cut(s) 121, 429
DdeI CTNAG 2 cut(s) 214, 668
DpnI GATC 4 cut(s) 17, 21, 465, 624
DpnII GATC 4 cut(s) 15, 19, 463, 622
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 528
Eco57I CTGAAG 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
Esp3I CGTCTC 1 cut(s) 601
FaeI CATG 3 cut(s) 200, 461, 690
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 645, 677
FatI CATG 3 cut(s) 196, 457, 686
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 3 cut(s) 89, 239, 522
FokI GGATG 2 cut(s) 41, 178
Fsp4HI GCNGC 3 cut(s) 89, 239, 522
GluI GCNGC 3 cut(s) 89, 239, 522
GsuI CTGGAG 1 cut(s) 669
HaeIII GGCC 2 cut(s) 521, 696
HapII CCGG 1 cut(s) 432
Hin1II CATG 3 cut(s) 200, 461, 690
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 4 cut(s) 73, 137, 299, 484
HpaII CCGG 1 cut(s) 432
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 3 cut(s) 8, 343, 429
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 5 cut(s) 23, 63, 292, 569, 648
Hpy8I GTNNAC 3 cut(s) 8, 343, 429
HpyAV CCTTC 3 cut(s) 355, 360, 502
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4IV ACGT 2 cut(s) 333, 594
HpyCH4V TGCA 9 cut(s) 40, 88, 191, 229, 241, 412, 478, 553, 709
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 235, 475, 545
HpyF3I CTNAG 2 cut(s) 214, 668
HpySE526I ACGT 2 cut(s) 333, 594
Hsp92II CATG 3 cut(s) 200, 461, 690
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 4 cut(s) 15, 19, 463, 622
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 100, 225
LweI GCATC 3 cut(s) 56, 200, 653
MaeII ACGT 2 cut(s) 333, 594
MaeIII GTNAC 1 cut(s) 126
MalI GATC 4 cut(s) 17, 21, 465, 624
MboI GATC 4 cut(s) 15, 19, 463, 622
MboII GAAGA 3 cut(s) 82, 479, 524
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 4 cut(s) 254, 339, 500, 642
MluCI AATT 2 cut(s) 222, 515
MlyI GAGTC 1 cut(s) 82
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 9 cut(s) 19, 176, 194, 288, 335, 447, 519, 582, 624
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 539
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 2 cut(s) 260, 433
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 4 cut(s) 197, 235, 475, 545
NciI CCSGG 1 cut(s) 433
NdeII GATC 4 cut(s) 15, 19, 463, 622
NlaIII CATG 3 cut(s) 200, 461, 690
NmeAIII GCCGAG 1 cut(s) 472
NspI RCATGY 1 cut(s) 461
NspV TTCGAA 1 cut(s) 359
PaeI GCATGC 1 cut(s) 461
PfeI GAWTC 3 cut(s) 137, 299, 484
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 3 cut(s) 90, 240, 523
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspPI GGNCC 2 cut(s) 528, 695
PsuI RGATCY 1 cut(s) 622
RsaI GTAC 2 cut(s) 122, 430
RsaNI GTAC 2 cut(s) 121, 429
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 89, 239, 522
Sau3AI GATC 4 cut(s) 15, 19, 463, 622
Sau96I GGNCC 2 cut(s) 528, 695
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 260, 433
SduI GDGCHC 4 cut(s) 254, 339, 500, 642
SfaNI GCATC 3 cut(s) 56, 200, 653
SfuI TTCGAA 1 cut(s) 359
SinI GGWCC 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 23, 316
SmoI CTYRAG 2 cut(s) 23, 316
SphI GCATGC 1 cut(s) 461
Sse9I AATT 2 cut(s) 222, 515
SsiI CCGC 1 cut(s) 522
SspI AATATT 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 258, 431
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 1 cut(s) 566
TaiI ACGT 2 cut(s) 336, 597
TaqI TCGA 3 cut(s) 18, 291, 359
TaqII GACCGA 1 cut(s) 545
TasI AATT 2 cut(s) 222, 515
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 3 cut(s) 137, 299, 484
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 1 cut(s) 485
TseI GCWGC 2 cut(s) 88, 238
TspDTI ATGAA 4 cut(s) 83, 275, 383, 428
TspGWI ACGGA 2 cut(s) 244, 600
TspRI CASTG 1 cut(s) 485
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 528
XceI RCATGY 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.