AT4G38230

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003972
Physical Location & Seq
Reverse (-)
17928677 .. 17931511
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38230.3

Sequence Viewer

Length: 1023 bp
ATGCATAGAGATCTAAAGCCCGAAAATTTCTTGTTGGTTAACAAGGACGACGACTTCTCTCTCAAAGCCATTGATTTTGGGCTTTCCGTGTTCTTCAAACCAGGTCAAATATTCGAGGATGTGGTTGGAAGTCCGTATTATGTTGCTCCTGAGGTTCTGCTCAAGCACTACGGACCCGAGGCTGATGTGTGGACCGCAGGGGTCATACTGTACATATTGGTGAGCGGAGTCCCTCCGTTCTGGGCAGAAACACAGCAAGGGATATTTGATGCAGTGCTGAAGGGACATATTGACTTTGACTCCGACCCTTGGCCCCTAATATCCGACTCTGCAAAAAACTTGATCCGTGGCATGCTATGTTCCCGGCCCTCTGAGCGACTGACAGCTCATCAAGTATTGCGCCATCCTTGGATCTGTGAAAATGGAGTTGCGCCAGACAGAGCACTAGATCCTGCTGTGCTTTCTCGTCTTAAACAGTTCTCTGCGATGAATAAACTAAAGCAGATGGCTTTACGGGTAATAGCTGAGAGTCTCTCGGAGGAAGAAATTGCTGGACTGAAGGAAATGTTCAAAGCAATGGACACGGATAACAGTGGAGCGATTACGTTTGACGAGCTGAAAGCCGGGTTGCGAAGATATGGTTCAACCCTCAAGGATACTGAGATTCGTGACCTTATGGAGGCTGCAGATATTGACAAGAGTGGGACCATAGATTATGGTGAATTCATTGCTGCAACAATCCATCTGAACAAATTAGAGCGTGAAGAACATCTTCTGTCAGCCTTCAGGTACTTTGACAAAGATGGGAGCGGTTACATCACTATCGATGAGCTACAGCACGCATGTGCTGAACAGGGAATGAGTGATGTCTTTCTAGAAGATGTGATCAAGGAAGTCGACCAAGACAATGATGGAAGGATTGACTACGGGGAGTTTGTAGCTATGATGCAGAAAGGCATTGTTGGAAGAACAATGAGGAAAAGCATAAACATGAGCATCAGGAACAATGCCGTCTCGCAGTGA

Protein Analysis

340

Amino Acids

38.2

Weight (kDa)

4.88

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 24 - 282 1.6e-74 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 26 - 276 5.9e-22 Protein tyrosine and serine/threonine kinase
EF-hand_1 PF00036 330 - 356 1.1e-07 EF hand domain
EF-hand_7 PF13499 330 - 389 1.2e-13 EF-hand domain pair
EF-hand_6 PF13405 330 - 358 1e-06 EF-hand domain
EF-hand_7 PF13499 399 - 461 1.1e-15 EF-hand domain pair
EF-hand_6 PF13405 402 - 427 5.1e-07 EF-hand domain
EF-hand_1 PF00036 403 - 427 5.2e-07 EF hand domain
EF-hand_1 PF00036 436 - 462 4.3e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 225, 810
AccI GTMKAC 1 cut(s) 897
AciI CCGC 3 cut(s) 195, 225, 810
AclWI GGATC 3 cut(s) 337, 419, 443
AcsI RAATTY 2 cut(s) 25, 722
AcuI CTGAAG 3 cut(s) 299, 578, 769
AfaI GTAC 2 cut(s) 212, 791
AfiI CCNNNNNNNGG 2 cut(s) 309, 679
AgsI TTSAA 3 cut(s) 97, 571, 645
AjnI CCWGG 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 843
AluBI AGCT 5 cut(s) 386, 524, 616, 832, 941
AluI AGCT 5 cut(s) 386, 524, 616, 832, 941
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 2 cut(s) 536, 1018
AlwI GGATC 3 cut(s) 337, 419, 443
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 311, 365
ApeKI GCWGC 2 cut(s) 683, 731
ApoI RAATTY 2 cut(s) 25, 722
Asp700I GAANNNNTTC 1 cut(s) 771
AspLEI GCGC 2 cut(s) 402, 433
AspS9I GGNCC 5 cut(s) 173, 192, 312, 366, 705
AsuC2I CCSGG 2 cut(s) 364, 625
AsuHPI GGTGA 2 cut(s) 232, 731
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 3 cut(s) 173, 192, 705
AxyI CCTNAGG 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 2 cut(s) 670, 718
BccI CCATC 5 cut(s) 411, 499, 750, 797, 905
BceAI ACGGC 1 cut(s) 995
BciT130I CCWGG 1 cut(s) 102
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 2 cut(s) 364, 625
BcoDI GTCTC 2 cut(s) 536, 1018
BfaI CTAG 2 cut(s) 446, 875
BfmI CTRYAG 2 cut(s) 684, 833
BfuI GTATCC 1 cut(s) 649
BglII AGATCT 1 cut(s) 10
BisI GCNGC 2 cut(s) 684, 732
BlsI GCNGC 2 cut(s) 685, 733
Bme1390I CCNGG 3 cut(s) 102, 364, 625
Bme18I GGWCC 3 cut(s) 173, 192, 705
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 5 cut(s) 173, 192, 312, 366, 705
BmiI GGNNCC 3 cut(s) 175, 314, 706
BmrFI CCNGG 3 cut(s) 102, 364, 625
BmsI GCATC 3 cut(s) 259, 936, 1005
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpuEI CTTGAG 2 cut(s) 146, 635
BpuMI CCSGG 2 cut(s) 364, 625
Bsa29I ATCGAT 1 cut(s) 825
BsaJI CCNNGG 4 cut(s) 177, 308, 346, 407
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 679
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 2 cut(s) 582, 726
BseBI CCWGG 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 825
BseDI CCNNGG 4 cut(s) 177, 308, 346, 407
BseGI GGATG 2 cut(s) 124, 403
BseLI CCNNNNNNNGG 2 cut(s) 309, 679
BseMI GCAATG 2 cut(s) 582, 726
BseMII CTCAG 4 cut(s) 141, 363, 516, 651
BseXI GCAGC 2 cut(s) 670, 718
BshFI GGCC 2 cut(s) 313, 367
BshVI ATCGAT 1 cut(s) 825
BsiHKAI GWGCWC 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 364, 624
BslFI GGGAC 3 cut(s) 215, 297, 718
BslI CCNNNNNNNGG 2 cut(s) 309, 679
BsmAI GTCTC 2 cut(s) 536, 1018
BsmBI CGTCTC 1 cut(s) 1018
BsmFI GGGAC 3 cut(s) 215, 297, 718
BsnI GGCC 2 cut(s) 313, 367
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 445
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 5 cut(s) 10, 342, 411, 448, 885
BspACI CCGC 3 cut(s) 195, 225, 810
BspANI GGCC 2 cut(s) 313, 367
BspCNI CTCAG 4 cut(s) 142, 364, 517, 652
BspDI ATCGAT 1 cut(s) 825
BspLI GGNNCC 3 cut(s) 175, 314, 706
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 3 cut(s) 337, 419, 443
BsrBI CCGCTC 2 cut(s) 225, 810
BsrDI GCAATG 2 cut(s) 582, 726
BsrGI TGTACA 1 cut(s) 210
BssECI CCNNGG 4 cut(s) 177, 308, 346, 407
BssMI GATC 5 cut(s) 10, 342, 411, 448, 885
BssT1I CCWWGG 2 cut(s) 308, 407
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 3 cut(s) 210, 477, 593
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 353, 840
BstDEI CTNAG 4 cut(s) 150, 372, 525, 660
BstDSI CCRYGG 1 cut(s) 346
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 2 cut(s) 124, 403
BstHHI GCGC 2 cut(s) 402, 433
BstKTI GATC 5 cut(s) 13, 345, 414, 451, 888
BstMAI GTCTC 2 cut(s) 536, 1018
BstMBI GATC 5 cut(s) 10, 342, 411, 448, 885
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 2 cut(s) 355, 846
BstSCI CCNGG 3 cut(s) 100, 362, 623
BstSFI CTRYAG 2 cut(s) 684, 833
BstV1I GCAGC 2 cut(s) 670, 718
BstX2I RGATCY 3 cut(s) 10, 411, 448
BstYI RGATCY 3 cut(s) 10, 411, 448
Bsu15I ATCGAT 1 cut(s) 825
Bsu36I CCTNAGG 1 cut(s) 150
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 2 cut(s) 313, 367
BsuTUI ATCGAT 1 cut(s) 825
BtgI CCRYGG 1 cut(s) 346
BtgZI GCGATG 1 cut(s) 500
BtsCI GGATG 2 cut(s) 124, 403
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 279, 598
Cac8I GCNNGC 2 cut(s) 353, 840
CfoI GCGC 2 cut(s) 402, 433
Cfr13I GGNCC 5 cut(s) 173, 192, 312, 366, 705
ClaI ATCGAT 1 cut(s) 825
CsiI ACCWGGT 1 cut(s) 100
Csp6I GTAC 2 cut(s) 211, 790
CviAII CATG 3 cut(s) 352, 843, 991
CviQI GTAC 2 cut(s) 211, 790
DdeI CTNAG 4 cut(s) 150, 372, 525, 660
DpnI GATC 5 cut(s) 12, 344, 413, 450, 887
DpnII GATC 5 cut(s) 10, 342, 411, 448, 885
Eco130I CCWWGG 2 cut(s) 308, 407
Eco47I GGWCC 3 cut(s) 173, 192, 705
Eco57I CTGAAG 3 cut(s) 299, 578, 769
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 308, 407
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 308, 407
Esp3I CGTCTC 1 cut(s) 1018
FaeI CATG 3 cut(s) 355, 846, 994
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 3 cut(s) 215, 297, 718
FatI CATG 3 cut(s) 351, 842, 990
FbaI TGATCA 1 cut(s) 885
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 2 cut(s) 684, 732
FokI GGATG 2 cut(s) 131, 390
Fsp4HI GCNGC 2 cut(s) 684, 732
FspBI CTAG 2 cut(s) 446, 875
GlaI GCGC 2 cut(s) 401, 432
GluI GCNGC 2 cut(s) 684, 732
HaeIII GGCC 2 cut(s) 313, 367
HapII CCGG 2 cut(s) 364, 624
HhaI GCGC 2 cut(s) 402, 433
Hin1II CATG 3 cut(s) 355, 846, 994
Hin6I GCGC 2 cut(s) 400, 431
HinP1I GCGC 2 cut(s) 400, 431
HincII GTYRAC 2 cut(s) 40, 898
HindII GTYRAC 2 cut(s) 40, 898
HinfI GANTC 5 cut(s) 228, 299, 326, 529, 664
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 2 cut(s) 364, 624
HphI GGTGA 2 cut(s) 232, 731
Hpy166II GTNNAC 3 cut(s) 40, 192, 898
Hpy188I TCNGA 5 cut(s) 304, 325, 373, 538, 747
Hpy188III TCNNGA 4 cut(s) 149, 668, 875, 1000
Hpy8I GTNNAC 3 cut(s) 40, 192, 898
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 274, 553, 793, 909
HpyCH4III ACNGT 3 cut(s) 210, 477, 593
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 4, 272, 332, 686, 734, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 4 cut(s) 150, 372, 525, 660
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 3 cut(s) 355, 846, 994
HspAI GCGC 2 cut(s) 400, 431
Ksp22I TGATCA 1 cut(s) 885
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 10, 342, 411, 448, 885
LmnI GCTCC 3 cut(s) 151, 596, 807
Lsp1109I GCAGC 2 cut(s) 670, 718
LweI GCATC 3 cut(s) 259, 936, 1005
MabI ACCWGGT 1 cut(s) 100
MaeI CTAG 2 cut(s) 446, 875
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 668, 812
MalI GATC 5 cut(s) 12, 344, 413, 450, 887
MbiI CCGCTC 2 cut(s) 225, 810
MboI GATC 5 cut(s) 10, 342, 411, 448, 885
MboII GAAGA 7 cut(s) 85, 554, 645, 764, 776, 890, 978
MflI RGATCY 3 cut(s) 10, 411, 448
MhlI GDGCHC 1 cut(s) 445
MluCI AATT 4 cut(s) 25, 546, 722, 752
MlyI GAGTC 4 cut(s) 237, 293, 320, 538
MmeI TCCRAC 4 cut(s) 106, 327, 348, 943
MnlI CCTC 9 cut(s) 109, 145, 172, 243, 379, 532, 659, 673, 969
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 2 cut(s) 39, 471
MslI CAYNNNNRTG 3 cut(s) 218, 843, 989
MspI CCGG 2 cut(s) 364, 624
MspR9I CCNGG 3 cut(s) 102, 364, 625
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 373
NciI CCSGG 2 cut(s) 364, 625
NdeII GATC 5 cut(s) 10, 342, 411, 448, 885
NlaIII CATG 3 cut(s) 355, 846, 994
NlaIV GGNNCC 3 cut(s) 175, 314, 706
NmuCI GTSAC 1 cut(s) 668
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 355, 846
OliI CACNNNNGTG 1 cut(s) 843
PaeI GCATGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 1 cut(s) 664
PkrI GCNGC 2 cut(s) 685, 733
PleI GAGTC 4 cut(s) 236, 293, 320, 537
PpsI GAGTC 4 cut(s) 236, 293, 320, 537
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 3 cut(s) 175, 314, 706
PspPI GGNCC 5 cut(s) 173, 192, 312, 366, 705
PstI CTGCAG 1 cut(s) 688
PsuI RGATCY 3 cut(s) 10, 411, 448
RsaI GTAC 2 cut(s) 212, 791
RsaNI GTAC 2 cut(s) 211, 790
RseI CAYNNNNRTG 3 cut(s) 218, 843, 989
SalI GTCGAC 1 cut(s) 896
SaqAI TTAA 2 cut(s) 39, 471
SatI GCNGC 2 cut(s) 684, 732
Sau3AI GATC 5 cut(s) 10, 342, 411, 448, 885
Sau96I GGNCC 5 cut(s) 173, 192, 312, 366, 705
SchI GAGTC 4 cut(s) 237, 293, 320, 538
ScrFI CCNGG 3 cut(s) 102, 364, 625
SduI GDGCHC 1 cut(s) 445
SexAI ACCWGGT 1 cut(s) 100
SfaNI GCATC 3 cut(s) 259, 936, 1005
SfcI CTRYAG 2 cut(s) 684, 833
SinI GGWCC 3 cut(s) 173, 192, 705
SmiMI CAYNNNNRTG 3 cut(s) 218, 843, 989
SmlI CTYRAG 2 cut(s) 161, 650
SmoI CTYRAG 2 cut(s) 161, 650
SphI GCATGC 1 cut(s) 355
Sse9I AATT 4 cut(s) 25, 546, 722, 752
SsiI CCGC 3 cut(s) 195, 225, 810
SspI AATATT 1 cut(s) 111
SspMI CTAG 2 cut(s) 446, 875
StyD4I CCNGG 3 cut(s) 100, 362, 623
StyI CCWWGG 2 cut(s) 308, 407
TaaI ACNGT 3 cut(s) 210, 477, 593
TaiI ACGT 1 cut(s) 608
TaqI TCGA 3 cut(s) 114, 825, 897
TasI AATT 4 cut(s) 25, 546, 722, 752
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 2 cut(s) 39, 471
Tru9I TTAA 2 cut(s) 39, 471
TscAI CASTG 2 cut(s) 279, 598
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 2 cut(s) 683, 731
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 2 cut(s) 503, 715
TspGWI ACGGA 6 cut(s) 76, 123, 186, 225, 335, 599
TspRI CASTG 2 cut(s) 279, 598
VpaK11BI GGWCC 3 cut(s) 173, 192, 705
XagI CCTNNNNNAGG 1 cut(s) 677
XapI RAATTY 2 cut(s) 25, 722
XbaI TCTAGA 1 cut(s) 874
XceI RCATGY 2 cut(s) 355, 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 2 cut(s) 446, 875
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.