Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003972

Physical Location & Seq

Genomic Coordinates Forward (+)

22807389 .. 22811885

Gene Structure 4497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1142300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGGGCAACACATGCCGTGGATCTTTCAGGGGGAAATATTTTCAGGGCTACAACCAGCCCGAAGAGCAATCTGCTTCCAATTCCAAGCCCAACACCGCGTCTGACCATTCGAATTCTGACCACTCCCAGTCCAGCTTGAAATCGCAGCACCACGGTCACCAAGAATCCCAAGACAGCCCCAACACCAAAACCAACAATAGTCATCCACCCCTCATTAGTCCCAGGAAAGATAACACCATGAGGAGAAGTGCTGATAACCAAAGTTATTATGTTTTGGGTCACAAGACCGCCAACATTCGTGATCTCTACACGTTGGGTCATAAATTAGGACAGGGACAATTTGGGACTACGTATTTATGCACTGAGATTGCTACTGGGGGTCAGTATGCATGTAAGTCTATCTCCAAAAGGAAGTTGATCTCAAAGGAGGATGTGGACGATGTTAGGAGGGAGATTCAGATAATGCACCATTTGGCTGGTCACAAGAATATTGTGACAATCAAGGGTGCTTACGAGGATTCACTGTATGTTCATATTGTAATGGAGCTTTGTTCTGGGGGTGAGTTGTTTGACCGCATCATCCAGAGAGGACATTACAGTGAGAGAAAGGCAGCTGAGCTAACAAGGATTATTGTTGGGGTTGTTGAAGCTTGCCATTCACTTGGTGTCATGCATAGGGATCTAAAACCTGAAAACTTCTTGTTGGTTAACAAGGACGATGATTTCTCTCTCAAGGCCATTGATTTTGGACTCTCAGTTTTCTTCAAACCAGGTCAAGTTTTCACTGATGTGGTCGGAAGCCCATATTATGTTGCTCCGGAGGTACTCCTCAAGCATTATGGACCTGAAGCAGATGTGTGGACTGCTGGAGTTATACTGTATATTCTGCTCAGCGGCGTGCCACCATTTTGGGCAGAAACCCAGCAGGGGATCTTTGATGCGGTCTTGAAGGGATATATTGACTTTGAATCAGAGCCATGGCCCGTAATATCTGACAGTGCAAAAGACCTAATCCGGAATATGCTATGTTCTAGGCCTTCAGACCGTTTGACTGCTCATGAAGTGCTATGCCACCCTTGGATTTGCGAAAATGGGGTTGCTCCTGACAGAGCCCTAGATCCAGCCGTACTTTCTCGTCTCAAAACATTTTCTGCTATGAACAAGTTAAAGAAGATGGCCTTACGGGTGATAGCTGAAAGCCTATCTGAGGAGGAGATTGCTGGGCTCAGAGAGATGTTCCAGGCAATGGATACTGATAATAGTGGTGCAATCACATTTGACGAACTTAAAGCTGGTTTGCGGAGATACGGCTCTACCATGAAGGATACAGAGATACGTGATCTTATGGATGCGGCTGATGTGGACAACAGTGGAACAATTGATTATGGTGAATTTATAGCTGCTACAGTTCATCTTAACAAACTAGAACGTGAAGAACATCTGATTGCAGCCTTCCAATACTTTGATAAGGATGGAAGTGGTTATATTACAGTTGATGAGCTCCAGCAAGCTTGTACAGAACATAACATGACTGACGTCCTTCTTGATGATATTATAAAAGAAGTTGATCAGGATAATGATGGAAGGATTGACTACGGTGAGTTTGTCGCCATGATGCAAAAGGGCAATGTGAAAGGAAACATGCCGGTTGGAAGACGGACTATGAGGAATAGTCTAAATGTGAGCATGAGAGATGCACCGGGAGCTCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

571

Amino Acids

63.74

Weight (kDa)

5.66

Isoelectric Point (pI)

42.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	103 - 361	1.1e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	104 - 355	4.3e-23	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	409 - 435	2.2e-07	EF hand domain
EF-hand_7	PF13499	409 - 468	1.9e-13	EF-hand domain pair
EF-hand_6	PF13405	409 - 437	1.5e-06	EF-hand domain
EF-hand_7	PF13499	478 - 540	7.2e-16	EF-hand domain pair
EF-hand_6	PF13405	481 - 507	2.8e-07	EF-hand domain
EF-hand_1	PF00036	482 - 507	8.4e-07	EF hand domain
EF-hand_5	PF13202	482 - 503	4.2e-06	EF hand
EF-hand_8	PF13833	493 - 541	8.1e-07	EF-hand domain pair
EF-hand_1	PF00036	515 - 541	1.5e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17290 AT2G17290 AT4G35310 AT4G35310 AT4G38230 AT4G38230 AT4G38230 AT4G38230
fragaria_vesca	FvH4_2g16880 FvH4_2g16880 FvH4_2g16880 FvH4_2g16880 FvH4_2g16880
malus_domestica	MD05G1140300.v1.1 MD05G1140600.v1.1 MD10G1142300.v1.1
prunus_persica	Prupe.8G180800_v2.0.a1 Prupe.8G180800_v2.0.a1
pyrus_communis	pycom05g13570
rosa_chinensis	RchiOBHm_Chr6g0281011
rosa_laevigata	RLG00000013006
rosa_multiflora	Rmu_co8419211.1_g000001 Rmu_sc0012869.1_g000002
rosa_roxburghii	Rroxscaffold_7G00187390
rosa_rugosa	Rorug06G0137800 Rorug06G0137900 Rorug06G0138000 Rorug06G0138100
rosa_samantha	Rh6AG250100 Rh6BG252400 Rh6CG252800 Rh6DG244500
rosa_wichuraiana	Rw6G021630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1556
AatII	GACGTC	1 cut(s)	1539
AccB7I	CCANNNNNTGG	1 cut(s)	1246
AccII	CGCG	1 cut(s)	98
AccIII	TCCGGA	2 cut(s)	817, 1014
AciI	CCGC	7 cut(s)	96, 288, 574, 894, 941, 1300, 1352
AclWI	GGATC	4 cut(s)	28, 687, 938, 1112
AcsI	RAATTY	2 cut(s)	112, 1391
AcuI	CTGAAG	2 cut(s)	867, 1023
AcyI	GRCGYC	1 cut(s)	1536
AdeI	CACNNNGTG	1 cut(s)	665
AfaI	GTAC	3 cut(s)	825, 1128, 1516
AfiI	CCNNNNNNNGG	3 cut(s)	927, 1207, 1246
AflIII	ACRYGT	1 cut(s)	309
AgsI	TTSAA	5 cut(s)	139, 647, 766, 949, 968
AjnI	CCWGG	3 cut(s)	221, 769, 1239
AleI	CACNNNNGTG	1 cut(s)	788
Alw21I	GWGCWC	2 cut(s)	1503, 1708
Alw26I	GTCTC	1 cut(s)	1142
AlwI	GGATC	4 cut(s)	28, 687, 938, 1112
Aor13HI	TCCGGA	2 cut(s)	817, 1014
AoxI	GGCC	4 cut(s)	735, 980, 1034, 1176
ApeKI	GCWGC	4 cut(s)	145, 611, 1400, 1448
ApoI	RAATTY	2 cut(s)	112, 1391
AspS9I	GGNCC	2 cut(s)	842, 981
AsuC2I	CCSGG	1 cut(s)	1701
AsuHPI	GGTGA	5 cut(s)	149, 572, 1198, 1400, 1610
AsuII	TTCGAA	1 cut(s)	110
AvaII	GGWCC	1 cut(s)	842
BanII	GRGCYC	4 cut(s)	1114, 1227, 1503, 1708
BbsI	GAAGAC	1 cut(s)	1660
Bbv12I	GWGCWC	2 cut(s)	1503, 1708
BbvI	GCAGC	4 cut(s)	157, 623, 1387, 1460
BccI	CCATC	3 cut(s)	1168, 1466, 1574
BceAI	ACGGC	2 cut(s)	1109, 1324
BciT130I	CCWGG	3 cut(s)	223, 771, 1241
BciVI	GTATCC	2 cut(s)	1243, 1318
BclI	TGATCA	1 cut(s)	1567
BcnI	CCSGG	1 cut(s)	1701
BcoDI	GTCTC	1 cut(s)	1142
BfaI	CTAG	3 cut(s)	1032, 1115, 1424
BfmI	CTRYAG	1 cut(s)	1404
BfuI	GTATCC	2 cut(s)	1243, 1318
BisI	GCNGC	6 cut(s)	146, 612, 895, 1353, 1401, 1449
BlpI	GCTNAGC	2 cut(s)	615, 890
BlsI	GCNGC	6 cut(s)	147, 613, 896, 1354, 1402, 1450
Bme1390I	CCNGG	4 cut(s)	223, 771, 1241, 1701
Bme18I	GGWCC	1 cut(s)	842
BmgT120I	GGNCC	2 cut(s)	842, 981
BmrFI	CCNGG	4 cut(s)	223, 771, 1241, 1701
BmrI	ACTGGG	2 cut(s)	121, 384
BmsI	GCATC	5 cut(s)	585, 928, 1339, 1605, 1684
BmuI	ACTGGG	2 cut(s)	121, 384
BoxI	GACNNNNGTC	1 cut(s)	1535
BpiI	GAAGAC	1 cut(s)	1660
BpmI	CTGGAG	2 cut(s)	888, 1487
Bpu1102I	GCTNAGC	2 cut(s)	615, 890
Bpu14I	TTCGAA	1 cut(s)	110
BpuEI	CTTGAG	2 cut(s)	716, 815
BpuMI	CCSGG	1 cut(s)	1701
BsaAI	YACGTR	2 cut(s)	351, 1337
BsaHI	GRCGYC	1 cut(s)	1536
BsaJI	CCNNGG	5 cut(s)	16, 151, 221, 977, 1076
BsaWI	WCCGGW	2 cut(s)	817, 1014
Bsc4I	CCNNNNNNNGG	3 cut(s)	927, 1207, 1246
Bse118I	RCCGGY	1 cut(s)	1645
Bse1I	ACTGG	2 cut(s)	127, 379
Bse3DI	GCAATG	2 cut(s)	1251, 1633
BseAI	TCCGGA	2 cut(s)	817, 1014
BseBI	CCWGG	3 cut(s)	223, 771, 1241
BseDI	CCNNGG	5 cut(s)	16, 151, 221, 977, 1076
BseGI	GGATG	5 cut(s)	202, 436, 579, 1354, 1477
BseLI	CCNNNNNNNGG	3 cut(s)	927, 1207, 1246
BseMI	GCAATG	2 cut(s)	1251, 1633
BseMII	CTCAG	6 cut(s)	354, 606, 768, 904, 1197, 1240
BseNI	ACTGG	2 cut(s)	127, 379
BseRI	GAGGAG	4 cut(s)	256, 818, 1223, 1226
BseXI	GCAGC	4 cut(s)	157, 623, 1387, 1460
BseYI	CCCAGC	2 cut(s)	921, 1220
Bsh1236I	CGCG	1 cut(s)	98
BshFI	GGCC	4 cut(s)	737, 982, 1036, 1178
BsiHKAI	GWGCWC	2 cut(s)	1503, 1708
BsiSI	CCGG	4 cut(s)	818, 1015, 1646, 1700
BslFI	GGGAC	3 cut(s)	204, 348, 358
BslI	CCNNNNNNNGG	3 cut(s)	927, 1207, 1246
BsmAI	GTCTC	1 cut(s)	1142
BsmBI	CGTCTC	1 cut(s)	1142
BsmFI	GGGAC	3 cut(s)	204, 348, 358
BsnI	GGCC	4 cut(s)	737, 982, 1036, 1178
Bsp119I	TTCGAA	1 cut(s)	110
Bsp1286I	GDGCHC	4 cut(s)	1114, 1227, 1503, 1708
Bsp13I	TCCGGA	2 cut(s)	817, 1014
Bsp1407I	TGTACA	1 cut(s)	1514
Bsp143I	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
Bsp1720I	GCTNAGC	2 cut(s)	615, 890
Bsp19I	CCATGG	1 cut(s)	977
BspACI	CCGC	7 cut(s)	96, 288, 574, 894, 941, 1300, 1352
BspANI	GGCC	4 cut(s)	737, 982, 1036, 1178
BspCNI	CTCAG	6 cut(s)	355, 607, 767, 903, 1198, 1239
BspEI	TCCGGA	2 cut(s)	817, 1014
BspFNI	CGCG	1 cut(s)	98
BspHI	TCATGA	1 cut(s)	1057
BspPI	GGATC	4 cut(s)	28, 687, 938, 1112
BspQI	GCTCTTC	1 cut(s)	57
BspT104I	TTCGAA	1 cut(s)	110
BsrDI	GCAATG	2 cut(s)	1251, 1633
BsrFI	RCCGGY	1 cut(s)	1645
BsrGI	TGTACA	1 cut(s)	1514
BsrI	ACTGG	2 cut(s)	127, 379
BssAI	RCCGGY	1 cut(s)	1645
BssECI	CCNNGG	5 cut(s)	16, 151, 221, 977, 1076
BssMI	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
BssNI	GRCGYC	1 cut(s)	1536
BssT1I	CCWWGG	2 cut(s)	977, 1076
Bst2UI	CCWGG	3 cut(s)	223, 771, 1241
Bst6I	CTCTTC	1 cut(s)	57
BstACI	GRCGYC	1 cut(s)	1536
BstAPI	GCANNNNNTGC	1 cut(s)	12
BstAUI	TGTACA	1 cut(s)	1514
BstBAI	YACGTR	2 cut(s)	351, 1337
BstBI	TTCGAA	1 cut(s)	110
BstC8I	GCNNGC	3 cut(s)	652, 899, 1509
BstDEI	CTNAG	6 cut(s)	363, 615, 754, 890, 1206, 1226
BstDSI	CCRYGG	3 cut(s)	16, 151, 977
BstEII	GGTNACC	1 cut(s)	155
BstENI	CCTNNNNNAGG	1 cut(s)	1205
BstF5I	GGATG	5 cut(s)	202, 436, 579, 1354, 1477
BstFNI	CGCG	1 cut(s)	98
BstKTI	GATC	8 cut(s)	23, 304, 420, 682, 933, 1120, 1342, 1570
BstMAI	GTCTC	1 cut(s)	1142
BstMBI	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
BstMWI	GCNNNNNNNGC	3 cut(s)	12, 64, 1703
BstNI	CCWGG	3 cut(s)	223, 771, 1241
BstNSI	RCATGY	3 cut(s)	15, 393, 1645
BstPAI	GACNNNNGTC	1 cut(s)	1535
BstPI	GGTNACC	1 cut(s)	155
BstSCI	CCNGG	4 cut(s)	221, 769, 1239, 1699
BstSFI	CTRYAG	1 cut(s)	1404
BstSNI	TACGTA	1 cut(s)	351
BstUI	CGCG	1 cut(s)	98
BstV1I	GCAGC	4 cut(s)	157, 623, 1387, 1460
BstV2I	GAAGAC	1 cut(s)	1660
BstX2I	RGATCY	4 cut(s)	20, 679, 930, 1117
BstXI	CCANNNNNNTGG	3 cut(s)	476, 662, 909
BstYI	RGATCY	4 cut(s)	20, 679, 930, 1117
BsuI	GTATCC	2 cut(s)	1243, 1318
BsuRI	GGCC	4 cut(s)	737, 982, 1036, 1178
BtgI	CCRYGG	3 cut(s)	16, 151, 977
BtsCI	GGATG	5 cut(s)	202, 436, 579, 1354, 1477
BtsIMutI	CAGTG	6 cut(s)	360, 521, 604, 783, 1003, 1375
Cac8I	GCNNGC	3 cut(s)	652, 899, 1509
CciI	TCATGA	1 cut(s)	1057
Cfr10I	RCCGGY	1 cut(s)	1645
Cfr13I	GGNCC	2 cut(s)	842, 981
CseI	GACGC	1 cut(s)	87
CsiI	ACCWGGT	1 cut(s)	769
Csp6I	GTAC	3 cut(s)	824, 1127, 1515
CviQI	GTAC	3 cut(s)	824, 1127, 1515
DdeI	CTNAG	6 cut(s)	363, 615, 754, 890, 1206, 1226
DpnI	GATC	8 cut(s)	22, 303, 419, 681, 932, 1119, 1341, 1569
DpnII	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
DraIII	CACNNNGTG	1 cut(s)	665
Eam1104I	CTCTTC	1 cut(s)	57
EarI	CTCTTC	1 cut(s)	57
Ecl136II	GAGCTC	2 cut(s)	1501, 1706
Eco105I	TACGTA	1 cut(s)	351
Eco130I	CCWWGG	2 cut(s)	977, 1076
Eco147I	AGGCCT	1 cut(s)	1036
Eco24I	GRGCYC	4 cut(s)	1114, 1227, 1503, 1708
Eco47I	GGWCC	1 cut(s)	842
Eco53kI	GAGCTC	2 cut(s)	1501, 1706
Eco57I	CTGAAG	2 cut(s)	867, 1023
Eco91I	GGTNACC	1 cut(s)	155
EcoICRI	GAGCTC	2 cut(s)	1501, 1706
EcoNI	CCTNNNNNAGG	1 cut(s)	1205
EcoO65I	GGTNACC	1 cut(s)	155
EcoRI	GAATTC	1 cut(s)	112
EcoRII	CCWGG	3 cut(s)	221, 769, 1239
EcoT14I	CCWWGG	2 cut(s)	977, 1076
EcoT22I	ATGCAT	2 cut(s)	391, 675
EcoT38I	GRGCYC	4 cut(s)	1114, 1227, 1503, 1708
ErhI	CCWWGG	2 cut(s)	977, 1076
Esp3I	CGTCTC	1 cut(s)	1142
FalI	AAGNNNNNCTT	2 cut(s)	1163, 1195
FaqI	GGGAC	3 cut(s)	204, 348, 358
FbaI	TGATCA	1 cut(s)	1567
Fnu4HI	GCNGC	6 cut(s)	146, 612, 895, 1353, 1401, 1449
FokI	GGATG	5 cut(s)	189, 443, 566, 1361, 1484
FriOI	GRGCYC	4 cut(s)	1114, 1227, 1503, 1708
Fsp4HI	GCNGC	6 cut(s)	146, 612, 895, 1353, 1401, 1449
FspBI	CTAG	3 cut(s)	1032, 1115, 1424
GluI	GCNGC	6 cut(s)	146, 612, 895, 1353, 1401, 1449
GsaI	CCCAGC	2 cut(s)	925, 1224
GsuI	CTGGAG	2 cut(s)	888, 1487
HaeIII	GGCC	4 cut(s)	737, 982, 1036, 1178
HapII	CCGG	4 cut(s)	818, 1015, 1646, 1700
HgaI	GACGC	1 cut(s)	87
Hin1I	GRCGYC	1 cut(s)	1536
HincII	GTYRAC	1 cut(s)	709
HindII	GTYRAC	1 cut(s)	709
HindIII	AAGCTT	2 cut(s)	648, 1509
HinfI	GANTC	5 cut(s)	164, 454, 518, 750, 968
HpaI	GTTAAC	1 cut(s)	709
HpaII	CCGG	4 cut(s)	818, 1015, 1646, 1700
HphI	GGTGA	5 cut(s)	149, 572, 1198, 1400, 1610
Hpy166II	GTNNAC	4 cut(s)	436, 709, 861, 1363
Hpy188III	TCNNGA	9 cut(s)	299, 583, 818, 946, 1015, 1058, 1103, 1544, 1571
Hpy8I	GTNNAC	4 cut(s)	436, 709, 861, 1363
HpyAV	CCTTC	6 cut(s)	943, 1047, 1315, 1462, 1550, 1578
HpyCH4IV	ACGT	5 cut(s)	311, 350, 1336, 1429, 1536
HpyCH4V	TGCA	9 cut(s)	360, 389, 466, 673, 1001, 1268, 1448, 1618, 1697
HpyF10VI	GCNNNNNNNGC	3 cut(s)	12, 64, 1703
HpyF3I	CTNAG	6 cut(s)	363, 615, 754, 890, 1206, 1226
HpySE526I	ACGT	5 cut(s)	311, 350, 1336, 1429, 1536
Hsp92I	GRCGYC	1 cut(s)	1536
Kpn2I	TCCGGA	2 cut(s)	817, 1014
Ksp22I	TGATCA	1 cut(s)	1567
KspAI	GTTAAC	1 cut(s)	709
Kzo9I	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
LguI	GCTCTTC	1 cut(s)	57
LmnI	GCTCC	5 cut(s)	544, 820, 1105, 1506, 1703
Lsp1109I	GCAGC	4 cut(s)	157, 623, 1387, 1460
LweI	GCATC	5 cut(s)	585, 928, 1339, 1605, 1684
MabI	ACCWGGT	1 cut(s)	769
MaeI	CTAG	3 cut(s)	1032, 1115, 1424
MaeII	ACGT	5 cut(s)	311, 350, 1336, 1429, 1536
MaeIII	GTNAC	4 cut(s)	155, 278, 479, 493
MalI	GATC	8 cut(s)	22, 303, 419, 681, 932, 1119, 1341, 1569
MboI	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
MboII	GAAGA	5 cut(s)	74, 754, 1183, 1445, 1665
MfeI	CAATTG	1 cut(s)	1377
MflI	RGATCY	4 cut(s)	20, 679, 930, 1117
MhlI	GDGCHC	4 cut(s)	1114, 1227, 1503, 1708
MluCI	AATT	6 cut(s)	79, 112, 323, 338, 1377, 1391
MlyI	GAGTC	1 cut(s)	744
MmeI	TCCRAC	2 cut(s)	775, 1630
Mph1103I	ATGCAT	2 cut(s)	391, 675
MroI	TCCGGA	2 cut(s)	817, 1014
MseI	TTAA	4 cut(s)	708, 1166, 1287, 1416
MslI	CAYNNNNRTG	2 cut(s)	597, 788
MspA1I	CMGCKG	2 cut(s)	614, 894
MspI	CCGG	4 cut(s)	818, 1015, 1646, 1700
MspR9I	CCNGG	4 cut(s)	223, 771, 1241, 1701
MunI	CAATTG	1 cut(s)	1377
MvaI	CCWGG	3 cut(s)	223, 771, 1241
MvnI	CGCG	1 cut(s)	98
MwoI	GCNNNNNNNGC	3 cut(s)	12, 64, 1703
NciI	CCSGG	1 cut(s)	1701
NcoI	CCATGG	1 cut(s)	977
NdeII	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
NmuCI	GTSAC	4 cut(s)	155, 278, 479, 493
NsiI	ATGCAT	2 cut(s)	391, 675
NspI	RCATGY	3 cut(s)	15, 393, 1645
NspV	TTCGAA	1 cut(s)	110
OliI	CACNNNNGTG	1 cut(s)	788
PagI	TCATGA	1 cut(s)	1057
PceI	AGGCCT	1 cut(s)	1036
PciSI	GCTCTTC	1 cut(s)	57
PfeI	GAWTC	4 cut(s)	164, 454, 518, 968
PflMI	CCANNNNNTGG	1 cut(s)	1246
PkrI	GCNGC	6 cut(s)	147, 613, 896, 1354, 1402, 1450
PleI	GAGTC	1 cut(s)	744
PpsI	GAGTC	1 cut(s)	744
Ppu21I	YACGTR	2 cut(s)	351, 1337
PshAI	GACNNNNGTC	1 cut(s)	1535
PsiI	TTATAA	1 cut(s)	1556
Psp124BI	GAGCTC	2 cut(s)	1503, 1708
Psp6I	CCWGG	3 cut(s)	221, 769, 1239
PspEI	GGTNACC	1 cut(s)	155
PspFI	CCCAGC	2 cut(s)	921, 1220
PspGI	CCWGG	3 cut(s)	221, 769, 1239
PspPI	GGNCC	2 cut(s)	842, 981
PsuI	RGATCY	4 cut(s)	20, 679, 930, 1117
PvuII	CAGCTG	1 cut(s)	614
RsaI	GTAC	3 cut(s)	825, 1128, 1516
RsaNI	GTAC	3 cut(s)	824, 1127, 1515
RseI	CAYNNNNRTG	2 cut(s)	597, 788
SacI	GAGCTC	2 cut(s)	1503, 1708
SapI	GCTCTTC	1 cut(s)	57
SaqAI	TTAA	4 cut(s)	708, 1166, 1287, 1416
SatI	GCNGC	6 cut(s)	146, 612, 895, 1353, 1401, 1449
Sau3AI	GATC	8 cut(s)	20, 301, 417, 679, 930, 1117, 1339, 1567
Sau96I	GGNCC	2 cut(s)	842, 981
SchI	GAGTC	1 cut(s)	744
ScrFI	CCNGG	4 cut(s)	223, 771, 1241, 1701
SduI	GDGCHC	4 cut(s)	1114, 1227, 1503, 1708
SexAI	ACCWGGT	1 cut(s)	769
SfaNI	GCATC	5 cut(s)	585, 928, 1339, 1605, 1684
SfcI	CTRYAG	1 cut(s)	1404
SfuI	TTCGAA	1 cut(s)	110
SinI	GGWCC	1 cut(s)	842
SmiMI	CAYNNNNRTG	2 cut(s)	597, 788
SmlI	CTYRAG	2 cut(s)	731, 830
SmoI	CTYRAG	2 cut(s)	731, 830
SnaBI	TACGTA	1 cut(s)	351
Sse9I	AATT	6 cut(s)	79, 112, 323, 338, 1377, 1391
SseBI	AGGCCT	1 cut(s)	1036
SsiI	CCGC	7 cut(s)	96, 288, 574, 894, 941, 1300, 1352
SspI	AATATT	2 cut(s)	38, 490
SspMI	CTAG	3 cut(s)	1032, 1115, 1424
SstI	GAGCTC	2 cut(s)	1503, 1708
StuI	AGGCCT	1 cut(s)	1036
StyD4I	CCNGG	4 cut(s)	221, 769, 1239, 1699
StyI	CCWWGG	2 cut(s)	977, 1076
TaiI	ACGT	5 cut(s)	314, 353, 1339, 1432, 1539
TaqI	TCGA	1 cut(s)	110
TasI	AATT	6 cut(s)	79, 112, 323, 338, 1377, 1391
TatI	WGTACW	1 cut(s)	1514
TauI	GCSGC	2 cut(s)	897, 1355
TfiI	GAWTC	4 cut(s)	164, 454, 518, 968
Tru1I	TTAA	4 cut(s)	708, 1166, 1287, 1416
Tru9I	TTAA	4 cut(s)	708, 1166, 1287, 1416
TscAI	CASTG	6 cut(s)	367, 528, 604, 790, 1003, 1375
TseFI	GTSAC	4 cut(s)	155, 278, 479, 493
TseI	GCWGC	4 cut(s)	145, 611, 1400, 1448
Tsp45I	GTSAC	4 cut(s)	155, 278, 479, 493
TspDTI	ATGAA	5 cut(s)	521, 1074, 1172, 1334, 1400
TspGWI	ACGGA	1 cut(s)	1672
TspRI	CASTG	6 cut(s)	367, 528, 604, 790, 1003, 1375
Van91I	CCANNNNNTGG	1 cut(s)	1246
VpaK11BI	GGWCC	1 cut(s)	842
XagI	CCTNNNNNAGG	1 cut(s)	1205
XapI	RAATTY	2 cut(s)	112, 1391
XceI	RCATGY	3 cut(s)	15, 393, 1645
XspI	CTAG	3 cut(s)	1032, 1115, 1424
ZraI	GACGTC	1 cut(s)	1537
Zsp2I	ATGCAT	2 cut(s)	391, 675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.