Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003972

Physical Location & Seq

Genomic Coordinates Forward (+)

27097558 .. 27101939

Gene Structure 4382 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1140300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGGCAACACATGCCGTGGATCTTTCAGGGGGAAATATTTTCAGGGCTCCAACCAGCCCGAAGAGCAATCTGCTTCCGTTTCCAAGCGCAACACCTCGTCTGACCATTCGAATTCTGACCACTCCCAGTCCAGTTTGAAATCGCAGCACCGCGGTCAGCAAGAATTCTCCAAAGACAGCCCCAAACCCAAAACCAACAATAGTCATCCACCCCTCATAAGTCCCAGGAAAGATAACATCATGAGGAGAAGTGCTGATAACCAAAGTTATTATGTGTTGGGTCACAAGACGGCCAACATTCGTGATCTTTACACGTTGGGTCATAAATTAGGACAGGGACAATTTGGGACTACGTATTTATGCACCGAGATTGCTACTGGCAGTCAGTATGCGTGTAAGTCTATCTCCAAGAGGAAGTTGATCTCGAAGGAGGATGTGGATGATGTTAGAAGGGAGATTCAGATAATGCACCATTTGGCTGGTCACAAGAATATTGTGACAATCAAGGGCGCTTATGAGGATTCACTGTATGTTCATATTGTAATGGAGCTTTGTGGCGGGGGTGAGTTGTTTGATCGCATTATCCAGAGGGGACATTACAGTGAGAGAAAGGCAGCTGAGTTGACACGGATTATTGTTGGTGTTGTTGAAGCTTGCCATTCACTTGGTGTCATGCATAGAGATCTGAAACCCGAAAACTTCTTGTTGGTTAACAAGGACAACGATTTTTCTCTCAAGGCCATTGATTTTGGACTCTCCGTTTTCTTCAAACCAGGTCAAGTTTTCACTGATGTGGTTGGAAGCCCATATTATGTTGCTCCGGAGGTACTCCTCAAGCATTATGGACCAGAAGCAGATGTGTGGACTGCAGGAGTTATACTGTATATTCTGCTCAGTGGTGTGCCGCCATTTTGGGCAGAAACCCAGCAGGGGATATTTGATGCGGTCTTGAAGGGATATATAGACTTTGAATCAGAGCCATGGCCTTTAATATCTAACAGTGCAAAAGACCTAATCCGGAAGATGCTATGTTCTAGGCCTTCAGACCGCTTAACTGCACATGAAGTGCTATGTCACCCTTGGATTTGCGAAAATGGGGTTGCTCCTGATAGAGCACTGGATCCAGCTGTACTTTCTCGTCTCAAAACGTTTTCTGCTATGAACAAGTTAAAGAAGATGGCTCTACGGGTAATAGCTGAAAGGCTATCTGAGGAGGAGATTGCTGGACTCAGAGAGATGTTTCAGGCTATGGATACTGATAATAGTGGTGCAATAACATTTGACGAACTTAAAGCTGGTTTGCGGAGATACGGCTCTACCATGAAGGATACAGAGATACGTGATCTTATGGATGCGGCTGATGTGGACAACAGTGGAACAATTGATTATGGGGAATTTATAGCTGCTACAGTTCATCTTAACAAACTAGAACGTGAAGAACATCTGGTTGCAGCCTTCCGATACTTTGATAAGGATGGAAGTGGTTATATTACGGTTGATGAGCTCCAGCAAGCTTGTACAGAACATCACATGACTGACGTCCTTCTTGAAGATATTATAAAAGAAGTTGATCAGGATAATGATGGAAGGATTGACTACGGTGAGTTTGTCGCCATGATGCAAAAGGGCAATGTGAAGGGCAACATGCCAATTGGAAGACGGACTATGAGGAACAGTCTAAATTTGAGCATGAGAGATGCACCAGGAGCTCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

572

Amino Acids

64.08

Weight (kDa)

6.18

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	104 - 362	7.8e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	105 - 356	4e-23	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	410 - 436	2.2e-07	EF hand domain
EF-hand_7	PF13499	410 - 469	1.9e-13	EF-hand domain pair
EF-hand_6	PF13405	410 - 438	1.5e-06	EF-hand domain
EF-hand_8	PF13833	425 - 472	8.5e-06	EF-hand domain pair
EF-hand_7	PF13499	479 - 541	1.3e-15	EF-hand domain pair
EF-hand_6	PF13405	482 - 508	2e-07	EF-hand domain
EF-hand_5	PF13202	482 - 504	3.4e-06	EF hand
EF-hand_1	PF00036	483 - 508	6.5e-07	EF hand domain
EF-hand_1	PF00036	516 - 542	2e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17290 AT2G17290 AT4G35310 AT4G35310 AT4G38230 AT4G38230 AT4G38230 AT4G38230
fragaria_vesca	FvH4_2g16880 FvH4_2g16880 FvH4_2g16880 FvH4_2g16880 FvH4_2g16880
malus_domestica	MD05G1140300.v1.1 MD05G1140600.v1.1 MD10G1142300.v1.1
prunus_persica	Prupe.8G180800_v2.0.a1 Prupe.8G180800_v2.0.a1
pyrus_communis	pycom05g13570
rosa_chinensis	RchiOBHm_Chr6g0281011
rosa_laevigata	RLG00000013006
rosa_multiflora	Rmu_co8419211.1_g000001 Rmu_sc0012869.1_g000002
rosa_roxburghii	Rroxscaffold_7G00187390
rosa_rugosa	Rorug06G0137800 Rorug06G0137900 Rorug06G0138000 Rorug06G0138100
rosa_samantha	Rh6AG250100 Rh6BG252400 Rh6CG252800 Rh6DG244500
rosa_wichuraiana	Rw6G021630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1559
AatII	GACGTC	1 cut(s)	1542
AccII	CGCG	1 cut(s)	153
AccIII	TCCGGA	2 cut(s)	820, 1017
AciI	CCGC	8 cut(s)	151, 153, 558, 905, 944, 1048, 1303, 1355
AclI	AACGTT	1 cut(s)	1148
AclWI	GGATC	3 cut(s)	28, 1115, 1128
AcoI	YGGCCR	1 cut(s)	291
AcsI	RAATTY	4 cut(s)	112, 164, 1394, 1681
AcuI	CTGAAG	1 cut(s)	1026
AcyI	GRCGYC	1 cut(s)	1539
AdeI	CACNNNGTG	1 cut(s)	668
AfaI	GTAC	3 cut(s)	828, 1131, 1519
AfiI	CCNNNNNNNGG	1 cut(s)	930
AflIII	ACRYGT	1 cut(s)	312
AgsI	TTSAA	6 cut(s)	139, 650, 769, 952, 971, 1550
AjnI	CCWGG	3 cut(s)	224, 772, 1702
AleI	CACNNNNGTG	1 cut(s)	791
Alw21I	GWGCWC	3 cut(s)	1117, 1506, 1711
Alw26I	GTCTC	1 cut(s)	1145
AlwI	GGATC	3 cut(s)	28, 1115, 1128
Aor13HI	TCCGGA	2 cut(s)	820, 1017
AoxI	GGCC	4 cut(s)	291, 738, 983, 1037
ApeKI	GCWGC	4 cut(s)	145, 614, 1403, 1451
ApoI	RAATTY	4 cut(s)	112, 164, 1394, 1681
AspLEI	GCGC	2 cut(s)	90, 512
AspS9I	GGNCC	1 cut(s)	845
AsuHPI	GGTGA	3 cut(s)	575, 1067, 1613
AsuII	TTCGAA	1 cut(s)	110
AvaII	GGWCC	1 cut(s)	845
BamHI	GGATCC	1 cut(s)	1120
BanII	GRGCYC	3 cut(s)	50, 1506, 1711
BbsI	GAAGAC	1 cut(s)	1663
Bbv12I	GWGCWC	3 cut(s)	1117, 1506, 1711
BbvI	GCAGC	4 cut(s)	157, 626, 1390, 1463
BccI	CCATC	3 cut(s)	1171, 1469, 1577
BceAI	ACGGC	2 cut(s)	306, 1327
BciT130I	CCWGG	3 cut(s)	226, 774, 1704
BciVI	GTATCC	2 cut(s)	1246, 1321
BclI	TGATCA	1 cut(s)	1570
BcoDI	GTCTC	1 cut(s)	1145
BfaI	CTAG	2 cut(s)	1035, 1427
BfmI	CTRYAG	2 cut(s)	867, 1407
BfoI	RGCGCY	1 cut(s)	513
BfuI	GTATCC	2 cut(s)	1246, 1321
BglII	AGATCT	1 cut(s)	682
BisI	GCNGC	6 cut(s)	146, 615, 905, 1356, 1404, 1452
BlsI	GCNGC	6 cut(s)	147, 616, 906, 1357, 1405, 1453
Bme1390I	CCNGG	3 cut(s)	226, 774, 1704
Bme18I	GGWCC	1 cut(s)	845
BmgT120I	GGNCC	1 cut(s)	845
BmiI	GGNNCC	2 cut(s)	49, 1122
BmrFI	CCNGG	3 cut(s)	226, 774, 1704
BmrI	ACTGGG	1 cut(s)	121
BmsI	GCATC	5 cut(s)	931, 1014, 1342, 1608, 1687
BmuI	ACTGGG	1 cut(s)	121
BoxI	GACNNNNGTC	1 cut(s)	1538
BpiI	GAAGAC	1 cut(s)	1663
BpmI	CTGGAG	1 cut(s)	1490
Bpu14I	TTCGAA	1 cut(s)	110
BpuEI	CTTGAG	2 cut(s)	719, 818
BsaAI	YACGTR	2 cut(s)	354, 1340
BsaHI	GRCGYC	1 cut(s)	1539
BsaJI	CCNNGG	5 cut(s)	16, 151, 224, 980, 1079
BsaWI	WCCGGW	2 cut(s)	820, 1017
Bsc4I	CCNNNNNNNGG	1 cut(s)	930
Bse1I	ACTGG	4 cut(s)	127, 132, 382, 1122
Bse3DI	GCAATG	1 cut(s)	1636
BseAI	TCCGGA	2 cut(s)	820, 1017
BseBI	CCWGG	3 cut(s)	226, 774, 1704
BseDI	CCNNGG	5 cut(s)	16, 151, 224, 980, 1079
BseGI	GGATG	5 cut(s)	205, 439, 445, 1357, 1480
BseLI	CCNNNNNNNGG	1 cut(s)	930
BseMI	GCAATG	1 cut(s)	1636
BseMII	CTCAG	4 cut(s)	609, 907, 1200, 1243
BseNI	ACTGG	4 cut(s)	127, 132, 382, 1122
BseRI	GAGGAG	4 cut(s)	259, 821, 1226, 1229
BseXI	GCAGC	4 cut(s)	157, 626, 1390, 1463
BseYI	CCCAGC	1 cut(s)	924
BsgI	GTGCAG	1 cut(s)	1041
Bsh1236I	CGCG	1 cut(s)	153
BshFI	GGCC	4 cut(s)	293, 740, 985, 1039
BsiHKAI	GWGCWC	3 cut(s)	1117, 1506, 1711
BsiSI	CCGG	2 cut(s)	821, 1018
BslFI	GGGAC	4 cut(s)	207, 351, 361, 606
BslI	CCNNNNNNNGG	1 cut(s)	930
BsmAI	GTCTC	1 cut(s)	1145
BsmBI	CGTCTC	1 cut(s)	1145
BsmFI	GGGAC	4 cut(s)	207, 351, 361, 606
BsnI	GGCC	4 cut(s)	293, 740, 985, 1039
Bsp119I	TTCGAA	1 cut(s)	110
Bsp1286I	GDGCHC	4 cut(s)	50, 1117, 1506, 1711
Bsp13I	TCCGGA	2 cut(s)	820, 1017
Bsp1407I	TGTACA	1 cut(s)	1517
Bsp143I	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
Bsp19I	CCATGG	1 cut(s)	980
BspACI	CCGC	8 cut(s)	151, 153, 558, 905, 944, 1048, 1303, 1355
BspANI	GGCC	4 cut(s)	293, 740, 985, 1039
BspCNI	CTCAG	4 cut(s)	610, 906, 1201, 1242
BspEI	TCCGGA	2 cut(s)	820, 1017
BspFNI	CGCG	1 cut(s)	153
BspHI	TCATGA	1 cut(s)	240
BspLI	GGNNCC	2 cut(s)	49, 1122
BspMAI	CTGCAG	1 cut(s)	871
BspPI	GGATC	3 cut(s)	28, 1115, 1128
BspQI	GCTCTTC	1 cut(s)	57
BspT104I	TTCGAA	1 cut(s)	110
BsrDI	GCAATG	1 cut(s)	1636
BsrGI	TGTACA	1 cut(s)	1517
BsrI	ACTGG	4 cut(s)	127, 132, 382, 1122
BssECI	CCNNGG	5 cut(s)	16, 151, 224, 980, 1079
BssMI	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
BssNI	GRCGYC	1 cut(s)	1539
BssT1I	CCWWGG	2 cut(s)	980, 1079
Bst2UI	CCWGG	3 cut(s)	226, 774, 1704
Bst4CI	ACNGT	9 cut(s)	528, 602, 882, 1001, 1373, 1411, 1495, 1601, 1676
Bst6I	CTCTTC	1 cut(s)	57
BstACI	GRCGYC	1 cut(s)	1539
BstAPI	GCANNNNNTGC	1 cut(s)	12
BstAUI	TGTACA	1 cut(s)	1517
BstBAI	YACGTR	2 cut(s)	354, 1340
BstBI	TTCGAA	1 cut(s)	110
BstC8I	GCNNGC	2 cut(s)	655, 1512
BstDEI	CTNAG	4 cut(s)	618, 893, 1209, 1229
BstDSI	CCRYGG	3 cut(s)	16, 151, 980
BstF5I	GGATG	5 cut(s)	205, 439, 445, 1357, 1480
BstFNI	CGCG	1 cut(s)	153
BstH2I	RGCGCY	1 cut(s)	513
BstHHI	GCGC	2 cut(s)	90, 512
BstKTI	GATC	8 cut(s)	23, 307, 423, 577, 685, 1123, 1345, 1573
BstMAI	GTCTC	1 cut(s)	1145
BstMBI	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
BstMWI	GCNNNNNNNGC	3 cut(s)	12, 64, 1706
BstNI	CCWGG	3 cut(s)	226, 774, 1704
BstNSI	RCATGY	2 cut(s)	15, 1648
BstPAI	GACNNNNGTC	1 cut(s)	1538
BstSCI	CCNGG	3 cut(s)	224, 772, 1702
BstSFI	CTRYAG	2 cut(s)	867, 1407
BstSNI	TACGTA	1 cut(s)	354
BstUI	CGCG	1 cut(s)	153
BstV1I	GCAGC	4 cut(s)	157, 626, 1390, 1463
BstV2I	GAAGAC	1 cut(s)	1663
BstX2I	RGATCY	3 cut(s)	20, 682, 1120
BstXI	CCANNNNNNTGG	2 cut(s)	479, 665
BstYI	RGATCY	3 cut(s)	20, 682, 1120
BsuI	GTATCC	2 cut(s)	1246, 1321
BsuRI	GGCC	4 cut(s)	293, 740, 985, 1039
BtgI	CCRYGG	3 cut(s)	16, 151, 980
BtsCI	GGATG	5 cut(s)	205, 439, 445, 1357, 1480
BtsIMutI	CAGTG	7 cut(s)	524, 607, 786, 901, 1006, 1115, 1378
Cac8I	GCNNGC	2 cut(s)	655, 1512
CciI	TCATGA	1 cut(s)	240
CfoI	GCGC	2 cut(s)	90, 512
Cfr13I	GGNCC	1 cut(s)	845
Cfr42I	CCGCGG	1 cut(s)	154
CsiI	ACCWGGT	1 cut(s)	772
Csp6I	GTAC	3 cut(s)	827, 1130, 1518
CviQI	GTAC	3 cut(s)	827, 1130, 1518
DdeI	CTNAG	4 cut(s)	618, 893, 1209, 1229
DpnI	GATC	8 cut(s)	22, 306, 422, 576, 684, 1122, 1344, 1572
DpnII	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
DraIII	CACNNNGTG	1 cut(s)	668
EaeI	YGGCCR	1 cut(s)	291
Eam1104I	CTCTTC	1 cut(s)	57
EarI	CTCTTC	1 cut(s)	57
Ecl136II	GAGCTC	2 cut(s)	1504, 1709
Eco105I	TACGTA	1 cut(s)	354
Eco130I	CCWWGG	2 cut(s)	980, 1079
Eco147I	AGGCCT	1 cut(s)	1039
Eco24I	GRGCYC	3 cut(s)	50, 1506, 1711
Eco47I	GGWCC	1 cut(s)	845
Eco53kI	GAGCTC	2 cut(s)	1504, 1709
Eco57I	CTGAAG	1 cut(s)	1026
EcoICRI	GAGCTC	2 cut(s)	1504, 1709
EcoRI	GAATTC	2 cut(s)	112, 164
EcoRII	CCWGG	3 cut(s)	224, 772, 1702
EcoT14I	CCWWGG	2 cut(s)	980, 1079
EcoT22I	ATGCAT	1 cut(s)	678
EcoT38I	GRGCYC	3 cut(s)	50, 1506, 1711
ErhI	CCWWGG	2 cut(s)	980, 1079
Esp3I	CGTCTC	1 cut(s)	1145
FaqI	GGGAC	4 cut(s)	207, 351, 361, 606
FauI	CCCGC	1 cut(s)	551
FbaI	TGATCA	1 cut(s)	1570
Fnu4HI	GCNGC	6 cut(s)	146, 615, 905, 1356, 1404, 1452
FokI	GGATG	5 cut(s)	192, 446, 452, 1364, 1487
FriOI	GRGCYC	3 cut(s)	50, 1506, 1711
Fsp4HI	GCNGC	6 cut(s)	146, 615, 905, 1356, 1404, 1452
FspBI	CTAG	2 cut(s)	1035, 1427
GlaI	GCGC	2 cut(s)	89, 511
GluI	GCNGC	6 cut(s)	146, 615, 905, 1356, 1404, 1452
GsaI	CCCAGC	1 cut(s)	928
GsuI	CTGGAG	1 cut(s)	1490
HaeII	RGCGCY	1 cut(s)	513
HaeIII	GGCC	4 cut(s)	293, 740, 985, 1039
HapII	CCGG	2 cut(s)	821, 1018
HhaI	GCGC	2 cut(s)	90, 512
Hin1I	GRCGYC	1 cut(s)	1539
Hin6I	GCGC	2 cut(s)	88, 510
HinP1I	GCGC	2 cut(s)	88, 510
HincII	GTYRAC	2 cut(s)	624, 712
HindII	GTYRAC	2 cut(s)	624, 712
HindIII	AAGCTT	2 cut(s)	651, 1512
HinfI	GANTC	5 cut(s)	457, 521, 753, 971, 1227
HpaI	GTTAAC	1 cut(s)	712
HpaII	CCGG	2 cut(s)	821, 1018
HphI	GGTGA	3 cut(s)	575, 1067, 1613
Hpy166II	GTNNAC	4 cut(s)	624, 712, 864, 1366
Hpy188I	TCNGA	9 cut(s)	103, 118, 462, 687, 976, 1045, 1210, 1232, 1460
Hpy8I	GTNNAC	4 cut(s)	624, 712, 864, 1366
HpyAV	CCTTC	9 cut(s)	421, 444, 946, 1050, 1318, 1465, 1553, 1581, 1630
HpyCH4III	ACNGT	9 cut(s)	528, 602, 882, 1001, 1373, 1411, 1495, 1601, 1676
HpyCH4IV	ACGT	6 cut(s)	314, 353, 1148, 1339, 1432, 1539
HpyF10VI	GCNNNNNNNGC	3 cut(s)	12, 64, 1706
HpyF3I	CTNAG	4 cut(s)	618, 893, 1209, 1229
HpySE526I	ACGT	6 cut(s)	314, 353, 1148, 1339, 1432, 1539
Hsp92I	GRCGYC	1 cut(s)	1539
HspAI	GCGC	2 cut(s)	88, 510
Kpn2I	TCCGGA	2 cut(s)	820, 1017
Ksp22I	TGATCA	1 cut(s)	1570
KspAI	GTTAAC	1 cut(s)	712
KspI	CCGCGG	1 cut(s)	154
Kzo9I	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
LguI	GCTCTTC	1 cut(s)	57
LmnI	GCTCC	6 cut(s)	53, 547, 823, 1108, 1509, 1706
Lsp1109I	GCAGC	4 cut(s)	157, 626, 1390, 1463
LweI	GCATC	5 cut(s)	931, 1014, 1342, 1608, 1687
MabI	ACCWGGT	1 cut(s)	772
MaeI	CTAG	2 cut(s)	1035, 1427
MaeII	ACGT	6 cut(s)	314, 353, 1148, 1339, 1432, 1539
MaeIII	GTNAC	4 cut(s)	281, 482, 496, 1073
MalI	GATC	8 cut(s)	22, 306, 422, 576, 684, 1122, 1344, 1572
MboI	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
MboII	GAAGA	7 cut(s)	74, 757, 1033, 1186, 1448, 1562, 1668
MfeI	CAATTG	2 cut(s)	1380, 1650
MflI	RGATCY	3 cut(s)	20, 682, 1120
MhlI	GDGCHC	4 cut(s)	50, 1117, 1506, 1711
MluCI	AATT	8 cut(s)	112, 164, 326, 341, 1380, 1394, 1650, 1681
MlyI	GAGTC	2 cut(s)	747, 1221
MmeI	TCCRAC	2 cut(s)	75, 778
Mph1103I	ATGCAT	1 cut(s)	678
MroI	TCCGGA	2 cut(s)	820, 1017
MseI	TTAA	6 cut(s)	711, 989, 1052, 1169, 1290, 1419
MslI	CAYNNNNRTG	2 cut(s)	600, 791
MspA1I	CMGCKG	3 cut(s)	153, 617, 1127
MspI	CCGG	2 cut(s)	821, 1018
MspR9I	CCNGG	3 cut(s)	226, 774, 1704
MunI	CAATTG	2 cut(s)	1380, 1650
MvaI	CCWGG	3 cut(s)	226, 774, 1704
MvnI	CGCG	1 cut(s)	153
MwoI	GCNNNNNNNGC	3 cut(s)	12, 64, 1706
NcoI	CCATGG	1 cut(s)	980
NdeII	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
NlaIV	GGNNCC	2 cut(s)	49, 1122
NmuCI	GTSAC	4 cut(s)	281, 482, 496, 1073
NsiI	ATGCAT	1 cut(s)	678
NspI	RCATGY	2 cut(s)	15, 1648
NspV	TTCGAA	1 cut(s)	110
OliI	CACNNNNGTG	1 cut(s)	791
PagI	TCATGA	1 cut(s)	240
PceI	AGGCCT	1 cut(s)	1039
PciSI	GCTCTTC	1 cut(s)	57
PfeI	GAWTC	3 cut(s)	457, 521, 971
PkrI	GCNGC	6 cut(s)	147, 616, 906, 1357, 1405, 1453
PleI	GAGTC	2 cut(s)	747, 1221
PpsI	GAGTC	2 cut(s)	747, 1221
Ppu21I	YACGTR	2 cut(s)	354, 1340
PshAI	GACNNNNGTC	1 cut(s)	1538
PsiI	TTATAA	1 cut(s)	1559
Psp124BI	GAGCTC	2 cut(s)	1506, 1711
Psp1406I	AACGTT	1 cut(s)	1148
Psp6I	CCWGG	3 cut(s)	224, 772, 1702
PspFI	CCCAGC	1 cut(s)	924
PspGI	CCWGG	3 cut(s)	224, 772, 1702
PspN4I	GGNNCC	2 cut(s)	49, 1122
PspPI	GGNCC	1 cut(s)	845
PstI	CTGCAG	1 cut(s)	871
PsuI	RGATCY	3 cut(s)	20, 682, 1120
PvuII	CAGCTG	2 cut(s)	617, 1127
RsaI	GTAC	3 cut(s)	828, 1131, 1519
RsaNI	GTAC	3 cut(s)	827, 1130, 1518
RseI	CAYNNNNRTG	2 cut(s)	600, 791
SacI	GAGCTC	2 cut(s)	1506, 1711
SacII	CCGCGG	1 cut(s)	154
SapI	GCTCTTC	1 cut(s)	57
SaqAI	TTAA	6 cut(s)	711, 989, 1052, 1169, 1290, 1419
SatI	GCNGC	6 cut(s)	146, 615, 905, 1356, 1404, 1452
Sau3AI	GATC	8 cut(s)	20, 304, 420, 574, 682, 1120, 1342, 1570
Sau96I	GGNCC	1 cut(s)	845
SchI	GAGTC	2 cut(s)	747, 1221
ScrFI	CCNGG	3 cut(s)	226, 774, 1704
SduI	GDGCHC	4 cut(s)	50, 1117, 1506, 1711
SexAI	ACCWGGT	1 cut(s)	772
SfaNI	GCATC	5 cut(s)	931, 1014, 1342, 1608, 1687
SfcI	CTRYAG	2 cut(s)	867, 1407
Sfr303I	CCGCGG	1 cut(s)	154
SfuI	TTCGAA	1 cut(s)	110
SgrBI	CCGCGG	1 cut(s)	154
SinI	GGWCC	1 cut(s)	845
SmiMI	CAYNNNNRTG	2 cut(s)	600, 791
SmlI	CTYRAG	2 cut(s)	734, 833
SmoI	CTYRAG	2 cut(s)	734, 833
SnaBI	TACGTA	1 cut(s)	354
Sse9I	AATT	8 cut(s)	112, 164, 326, 341, 1380, 1394, 1650, 1681
SseBI	AGGCCT	1 cut(s)	1039
SsiI	CCGC	8 cut(s)	151, 153, 558, 905, 944, 1048, 1303, 1355
SspI	AATATT	2 cut(s)	38, 493
SspMI	CTAG	2 cut(s)	1035, 1427
SstI	GAGCTC	2 cut(s)	1506, 1711
StuI	AGGCCT	1 cut(s)	1039
StyD4I	CCNGG	3 cut(s)	224, 772, 1702
StyI	CCWWGG	2 cut(s)	980, 1079
TaaI	ACNGT	9 cut(s)	528, 602, 882, 1001, 1373, 1411, 1495, 1601, 1676
TaiI	ACGT	6 cut(s)	317, 356, 1151, 1342, 1435, 1542
TaqI	TCGA	2 cut(s)	110, 425
TasI	AATT	8 cut(s)	112, 164, 326, 341, 1380, 1394, 1650, 1681
TatI	WGTACW	2 cut(s)	1129, 1517
TauI	GCSGC	2 cut(s)	907, 1358
TfiI	GAWTC	3 cut(s)	457, 521, 971
Tru1I	TTAA	6 cut(s)	711, 989, 1052, 1169, 1290, 1419
Tru9I	TTAA	6 cut(s)	711, 989, 1052, 1169, 1290, 1419
TscAI	CASTG	7 cut(s)	531, 607, 793, 901, 1006, 1122, 1378
TseFI	GTSAC	4 cut(s)	281, 482, 496, 1073
TseI	GCWGC	4 cut(s)	145, 614, 1403, 1451
Tsp45I	GTSAC	4 cut(s)	281, 482, 496, 1073
TspDTI	ATGAA	5 cut(s)	524, 1077, 1175, 1337, 1403
TspGWI	ACGGA	4 cut(s)	67, 643, 748, 1675
TspRI	CASTG	7 cut(s)	531, 607, 793, 901, 1006, 1122, 1378
VpaK11BI	GGWCC	1 cut(s)	845
XapI	RAATTY	4 cut(s)	112, 164, 1394, 1681
XceI	RCATGY	2 cut(s)	15, 1648
XspI	CTAG	2 cut(s)	1035, 1427
ZraI	GACGTC	1 cut(s)	1540
Zsp2I	ATGCAT	1 cut(s)	678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.