Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003972

Physical Location & Seq

Genomic Coordinates Forward (+)

27122034 .. 27127892

Gene Structure 5859 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1140600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3912 bp

ATGGTTCTACCCGTATTCTACCACATCAATCCATCTCATGTACGAAACCAACACGGGAGTTATGCGGATGCATTTGCTCAACTTGAAAAGCGTTTCGAGGACGGTATCGATAAGGTGCACAAGTGGAGGGATGCTTTGACGACTGCAGGCAAACTATCTGGGTTTCATCATTCAAACAAATCCGGGACGGAGGCAGATCTAATTAAGAATGTTGTCGATCGTATTTGGACCAAATTGATTTGTGAATCATCATGTGATTTAGAGGGCCTGGTTGGAATTGAAAGCCGCATTCAGCAAATTGAAGAGCTATTGGGCATTCATTCACAAGACGCTTGCATCACTGTTGGTATTTGGGGAATGGGTGGCATTGGCAAGACCACCCTCGCTGAAACTGTATTCCACAGACTTTCTTCTAAATTTGATGCTTCTTGTTTTCTTAAGAATGTTAGGGAGAACTCAGAAAAAGCAGATGGACTAGATCACTTGGAAAAAAAACTTCTTAAGGAGATATTAAAGGGAGAAGGTCTATCCATGGGATCAACTTTTGTTCGAGATAGGCTCAAGTGTACAAAGGTCCTCATCGTTCTTGATGATGTGAGTGATTCAATACAGCTGGAACGTTTAGTTGGCAAGCGTCTTCGGTATGCCACTGGAAGTAGAATCATTATCACAAGTAAGGATAAGAGAACACTTACTGAAGAGGGTAAGATCTACGAGGTTGAGGGATTAAAACGGGATGATGCTCTTCAGCTCTTCTGTTCTCACGCTTTCAAGAATAAAAGTACTCCTATAACAGATTATAAGGAGTTGGCAGAAAAGGCGGTGGACTATGCTGGAGGCGTTCCTTTAGCTCTTAAACTTCTGGGGTCCTTGTTCTTCAGTTGCAAGAGCAAAGAAGAATGGGAAGAGGAATTGGACAAACTGAAAGAGTTTCCCAATGAAAAGCTCCAGAGAGTGTTGAGACTAAGTTATGATGGATTGGAAAGAAATGAGAAGGAGATATTTTTGGATATAGCATGTTTTCATAAAGGGAATGAAGTGGAAGAGGTAAGAAAAAGGTTGGCTATCCGTGGACGCTTTGCGGCGGCTGGAATTAGAGTTCTCGTTGATAAGTCTCTCATATCAATTAAGTCAATAGGATGGGAAATTATAGAGATGCATGATCTGCTTGAAGAATTGGGAAGAACAATTGTTCAGGAACAATGTATTGAAGATCCCGGAAAACGGAATAGGTTGTTCAACGATGAGGATATATATCGTGTACTGACGATTAACAAGGAAACTCCAAATGTTCAAGCCATAATGTTTGATTGGTCAAAGATTAAAAAGCGAGCATTGAAAAGTGCAGACCTCAAAGTGATGTCTAACCTCAAAGTGATGTCTAACCTAAAAATGCTAATGGTGGATAGTTTTGTCGCTTCTCTAGACCTTCCCGACTCTTTTGTCGCTTCTCTAGACCTTCCCGACTCTCTTCGTTATCTCTACTGGCCTCGTTATCCACTGGAATCTTTGCCGTCAAATTTTTCTCCTGAAAATCTAGTTGAGTTTCATATGCGATATAGCCAAGTTAAGAAGCTTTGTAAAGAAGACCAGATACTTGTGAACTTGGAAGTGATCGATCTGAAGCACTCTTGGTACCTATCTGAACTTCCAAATCTCTCTGAGAGTCAAAAAATTGTGAGCATAGATCTCTTTGGCTGTAGAAGTTTGGTTGAAATTCCTTGGTCTTTTCAACGTCTTGACAAGCTTACTCATCTTGATGTTGGAGGCTGCACCAGTCTCGAGTATCTTCCAGAGATGCCAAGGAATATTATATACTTAGATTTACAAAACAGTGGTATAAAGGAGTTGCCCGAATCAGTTTGGTCTCATGAAAATATTTCTCACTTGGATATTACTGGTTGTAAAGACCTTGAGAAACTTCCAAGCAACAAGTGTAAGCTGAAAGTCTCTGGTTGTTTTAATCTAGATGGCTGCACATCTCTTGGTGAGTTTTCTGAGCTTCCCAGGGATATAAGTAAATTATCATTGGTTGGTTGCATGAGACTTGTGAGTCTACCAGCCAACATTTGTAAGTTGAAATATCTCGAGGGACTCAATCTCTCTGGGTGCTCTAAACTTCAAAACTTCCCCGAGATATTGGAGCCAATGGAACATTTGAAGCACTTAAATTTAAGTCAAACAGCGATTAAAGAGCTACACTCATCAATCGAGTTTCTCCCTGCGCTCAAAACACTTGAACTACAAAGTTGCAAAAGGCTTTCAAGTATCCCAAAAAGCATTTGTAAGTTGAAATATCTCGAGGAACTCAATCTGTCTGGCTGCTCCAAACTTAAAAACTTCCCAGAGATCTTGGAGCCAATGGAACATTTGAAGTCCTTAGATTTAAGTCAAACAGCGGTTGAAAAGCTACACTTATCAATCGAGTTTCTCCCTAACATCATGAGGAGAAGTGCTGATAACCAAAGTTATTATGTGTTGGGTCACAAGACCGCCAACATTCGTGATCTTTACACGTTGGGTCATAAATTAGGACAGGGACAATTTGGGTCTACGTATTTATGCATTGAGATTGCTACTGGCTGTCAGTATGCGTGTAAGTCTATCTCCAAGAGGAAGTTGATCTCGAAGGAGGATGTGGACGATGTTAGGATGGAGATTCAGATAATGCACCATTTGGCTGGTCACAAGAATATTGTGACAATCAAGGGCGCTTATGAGGATTCACTGTATGTTCATATTGTAATGGAGCTTTGTGGCGGGGGTGAGTTGTTTGATCACATTGTCCAGAGGGTTTATTACAGTGAGAGAAAGGCAGCTGAGTTGACACGGATTATTGTTGGTGTTGTTGAAGCTTGCCATTCACTTGGTGTCATGCATAGAGATATGAAACCCGAAAACTTCTTGTTGGTTAACAAGGACGATGATTTTTCTCTCAAGGCCATTGATTTTGGACTCTCAGTTTTCTTCAAACCGGGTCAAGTTTTCACTGATGTGGTTGGAAGCCCATATTATGTTGCTCCGGAGGTACTCCTCAATCATTATGGACCAGAAGCGGATGTGTGGACTGCAGGAGTTACACTGTATATTCTGCTCAGTGGTGTGGTACCATTTTGGGCAGAAACCCAGCAGGGGATATTTGATGCGGTCTTGAAGGGATATATAGACTTTGAATCAGAGCCATGGCCTGAAATATCTGACAGTGCAAAAGACCTAATCCGGAAGATGCTATGTTCTAGGCCTTCAGACCGCTTAACTGCACATGAAGTGCTATGTCACCCTTGGATTTGCGAAAATGGGGTTGCTTCTGATAGAGATCCAGTTGTACTTTCTCGTCTCAAAACGTTTTCTGCTATGAACAAGTTAAAGAAGATGGCTTTACTGGTAATAGCTGAAAGCCTATCTGCGGAGGAGATTGCTGGACTCAGAGAGATGTTTCAGGCTATGGATACTGATAATAGTGGTGCAATAACATTTGATGAACTTAAAGCTGGTTTGCGGAGATACGGCTCTACCATGAAGGATACAGAGATATGTGATCTTATGGCTGCGGCTGATCTGGACCACAGTGGAACAATTGATTATGGGGAATTTCTAGCTGCTACAATTCATCTTAACAAACTAGAATGTGAAGAACATCTGGTTGCAGCCTTCCGATACTTTGATAAGGATGGAAGTGGTTATATTACGGTTGATGAGCTCCAGCAAGCTTGTACAGAACATAACATGACTGACGTCCTTCTTGAAGATATTATAAAAGAAGTTGATCAGGATAATGATGGAAGGATTGACTACAGTGAGTTTGTCGCCATGATGCAAAGGGGCAATGTGAAAGGCAACATGCCAATTGGAAGACGGACTATGAGGAACAGTCTAAATTTGAGCATGAGAGATGCACCAGGAGCTCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

1304

Amino Acids

147.34

Weight (kDa)

5.78

Isoelectric Point (pI)

42.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	1 - 93	8.8e-14	TIR domain
NB-ARC	PF00931	98 - 261	6.6e-17	NB-ARC domain
WHD_ROQ1	PF23282	328 - 401	9.5e-19	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	690 - 803	3.7e-10	Leucine-rich repeat region
LRR_13	PF23286	694 - 780	6.9e-10	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_Zer-1	PF25013	712 - 802	1.9e-06	Zer-1-like, Leucine-rich repeats
Pkinase	PF00069	838 - 1096	2.3e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	839 - 1090	6.6e-22	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	1142 - 1168	5.7e-07	EF hand domain
EF-hand_7	PF13499	1142 - 1201	9.9e-12	EF-hand domain pair
EF-hand_6	PF13405	1142 - 1170	4.1e-06	EF-hand domain
EF-hand_7	PF13499	1211 - 1273	4.2e-16	EF-hand domain pair
EF-hand_6	PF13405	1214 - 1240	5.2e-07	EF-hand domain
EF-hand_5	PF13202	1214 - 1236	8.7e-06	EF hand
EF-hand_1	PF00036	1215 - 1240	1.7e-06	EF hand domain
EF-hand_1	PF00036	1248 - 1274	2e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17290 AT2G17290 AT4G35310 AT4G35310 AT4G38230 AT4G38230 AT4G38230 AT4G38230
fragaria_vesca	FvH4_2g16880 FvH4_2g16880 FvH4_2g16880 FvH4_2g16880 FvH4_2g16880
malus_domestica	MD05G1140300.v1.1 MD05G1140600.v1.1 MD10G1142300.v1.1
prunus_persica	Prupe.8G180800_v2.0.a1 Prupe.8G180800_v2.0.a1
pyrus_communis	pycom05g13570
rosa_chinensis	RchiOBHm_Chr6g0281011
rosa_laevigata	RLG00000013006
rosa_multiflora	Rmu_co8419211.1_g000001 Rmu_sc0012869.1_g000002
rosa_roxburghii	Rroxscaffold_7G00187390
rosa_rugosa	Rorug06G0137800 Rorug06G0137900 Rorug06G0138000 Rorug06G0138100
rosa_samantha	Rh6AG250100 Rh6BG252400 Rh6CG252800 Rh6DG244500
rosa_wichuraiana	Rw6G021630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	801, 3755
AasI	GACNNNNNNGTC	2 cut(s)	1442, 2052
AatII	GACGTC	1 cut(s)	3738
Acc65I	GGTACC	2 cut(s)	1635, 3106
AccB1I	GGYRCC	2 cut(s)	1635, 3106
AccI	GTMKAC	2 cut(s)	2056, 2552
AccIII	TCCGGA	2 cut(s)	3022, 3219
AclI	AACGTT	2 cut(s)	619, 3344
AclWI	GGATC	3 cut(s)	544, 1208, 3311
AcsI	RAATTY	6 cut(s)	416, 1519, 1716, 2170, 3590, 3877
AcuI	CTGAAG	5 cut(s)	717, 731, 862, 1643, 3228
AcyI	GRCGYC	1 cut(s)	3735
AdeI	CACNNNGTG	1 cut(s)	2870
AfaI	GTAC	9 cut(s)	42, 568, 784, 1263, 1637, 3030, 3108, 3327, 3715
AfiI	CCNNNNNNNGG	3 cut(s)	1224, 3132, 3406
AflII	CTTAAG	2 cut(s)	437, 500
AflIII	ACRYGT	1 cut(s)	2514
AjnI	CCWGG	3 cut(s)	267, 2006, 3898
AjuI	GAANNNNNNNTTGG	2 cut(s)	609, 641
AleI	CACNNNNGTG	1 cut(s)	2993
AloI	GAACNNNNNNTCC	2 cut(s)	1083, 1115
Alw21I	GWGCWC	4 cut(s)	120, 2114, 3702, 3907
Alw26I	GTCTC	7 cut(s)	955, 1119, 1784, 1872, 1954, 2038, 3341
Alw44I	GTGCAC	1 cut(s)	116
AlwI	GGATC	3 cut(s)	544, 1208, 3311
Ama87I	CYCGRG	4 cut(s)	1781, 2087, 2132, 2300
Aor13HI	TCCGGA	2 cut(s)	3022, 3219
AoxI	GGCC	5 cut(s)	265, 1487, 2940, 3185, 3239
ApaLI	GTGCAC	1 cut(s)	116
ApeKI	GCWGC	7 cut(s)	1770, 1974, 2322, 2816, 3548, 3599, 3647
ApoI	RAATTY	6 cut(s)	416, 1519, 1716, 2170, 3590, 3877
Asp718I	GGTACC	2 cut(s)	1635, 3106
AspLEI	GCGC	2 cut(s)	2227, 2714
AspS9I	GGNCC	6 cut(s)	228, 265, 574, 867, 3047, 3562
AsuC2I	CCSGG	3 cut(s)	184, 1218, 2976
AsuHPI	GGTGA	3 cut(s)	2000, 2777, 3269
AvaI	CYCGRG	4 cut(s)	1781, 2087, 2132, 2300
AvaII	GGWCC	5 cut(s)	228, 574, 867, 3047, 3562
BaeGI	GKGCMC	1 cut(s)	120
BanI	GGYRCC	2 cut(s)	1635, 3106
BanII	GRGCYC	2 cut(s)	3702, 3907
BarI	GAAGNNNNNNTAC	2 cut(s)	1578, 1610
BbsI	GAAGAC	3 cut(s)	629, 1593, 3859
Bbv12I	GWGCWC	4 cut(s)	120, 2114, 3702, 3907
BbvI	GCAGC	7 cut(s)	1757, 1961, 2309, 2828, 3535, 3586, 3659
BccI	CCATC	9 cut(s)	40, 464, 968, 1134, 1964, 2647, 3367, 3665, 3773
BceAI	ACGGC	2 cut(s)	1498, 3523
BcgI	CGANNNNNNTGC	2 cut(s)	1762, 1796
BciT130I	CCWGG	3 cut(s)	269, 2008, 3900
BciVI	GTATCC	3 cut(s)	2279, 3442, 3517
BclI	TGATCA	2 cut(s)	2776, 3766
BcnI	CCSGG	3 cut(s)	184, 1218, 2976
BcoDI	GTCTC	7 cut(s)	955, 1119, 1784, 1872, 1954, 2038, 3341
BfaI	CTAG	8 cut(s)	476, 1424, 1454, 1538, 1967, 3237, 3596, 3623
BfmI	CTRYAG	4 cut(s)	144, 1699, 3069, 3793
BfoI	RGCGCY	1 cut(s)	2715
BfrI	CTTAAG	2 cut(s)	437, 500
BfuI	GTATCC	3 cut(s)	2279, 3442, 3517
BglII	AGATCT	4 cut(s)	196, 708, 1687, 2349
BmcAI	AGTACT	1 cut(s)	784
Bme1390I	CCNGG	6 cut(s)	184, 269, 1218, 2008, 2976, 3900
Bme18I	GGWCC	5 cut(s)	228, 574, 867, 3047, 3562
BmeT110I	CYCGRG	4 cut(s)	1781, 2087, 2132, 2300
BmgT120I	GGNCC	6 cut(s)	228, 265, 574, 867, 3047, 3562
BmiI	GGNNCC	5 cut(s)	868, 1637, 2145, 2358, 3108
BmrFI	CCNGG	6 cut(s)	184, 269, 1218, 2008, 2976, 3900
BoxI	GACNNNNGTC	1 cut(s)	3734
BpiI	GAAGAC	3 cut(s)	629, 1593, 3859
BplI	GAGNNNNNCTC	4 cut(s)	543, 575, 2186, 2218
BpmI	CTGGAG	3 cut(s)	855, 932, 3686
BpuEI	CTTGAG	3 cut(s)	545, 1934, 2921
BpuMI	CCSGG	3 cut(s)	184, 1218, 2976
Bsa29I	ATCGAT	2 cut(s)	108, 1617
BsaAI	YACGTR	1 cut(s)	2556
BsaBI	GATNNNNATC	2 cut(s)	1254, 3315
BsaHI	GRCGYC	1 cut(s)	3735
BsaI	GGTCTC	1 cut(s)	1872
BsaJI	CCNNGG	8 cut(s)	531, 1069, 1721, 1802, 2006, 2007, 3182, 3281
BsaWI	WCCGGW	2 cut(s)	3022, 3219
Bsc4I	CCNNNNNNNGG	3 cut(s)	1224, 3132, 3406
Bse1I	ACTGG	8 cut(s)	655, 1490, 1506, 1776, 1903, 2584, 3320, 3387
Bse3DI	GCAATG	1 cut(s)	3832
Bse8I	GATNNNNATC	2 cut(s)	1254, 3315
BseAI	TCCGGA	2 cut(s)	3022, 3219
BseBI	CCWGG	3 cut(s)	269, 2008, 3900
BseCI	ATCGAT	2 cut(s)	108, 1617
BseDI	CCNNGG	8 cut(s)	531, 1069, 1721, 1802, 2006, 2007, 3182, 3281
BseGI	GGATG	8 cut(s)	73, 136, 742, 1145, 2641, 2658, 3064, 3676
BseJI	GATNNNNATC	2 cut(s)	1254, 3315
BseLI	CCNNNNNNNGG	3 cut(s)	1224, 3132, 3406
BseMI	GCAATG	1 cut(s)	3832
BseMII	CTCAG	7 cut(s)	471, 1653, 1989, 2811, 2973, 3109, 3439
BseNI	ACTGG	8 cut(s)	655, 1490, 1506, 1776, 1903, 2584, 3320, 3387
BseRI	GAGGAG	3 cut(s)	2461, 3023, 3425
BseSI	GKGCMC	1 cut(s)	120
BseXI	GCAGC	7 cut(s)	1757, 1961, 2309, 2828, 3535, 3586, 3659
BseYI	CCCAGC	1 cut(s)	3126
BsgI	GTGCAG	4 cut(s)	1365, 1756, 1960, 3243
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	5 cut(s)	267, 1489, 2942, 3187, 3241
BshNI	GGYRCC	2 cut(s)	1635, 3106
BshVI	ATCGAT	2 cut(s)	108, 1617
BsiEI	CGRYCG	1 cut(s)	220
BsiHKAI	GWGCWC	4 cut(s)	120, 2114, 3702, 3907
BsiHKCI	CYCGRG	4 cut(s)	1781, 2087, 2132, 2300
BsiSI	CCGG	5 cut(s)	183, 1218, 2975, 3023, 3220
BslFI	GGGAC	3 cut(s)	199, 2106, 2553
BslI	CCNNNNNNNGG	3 cut(s)	1224, 3132, 3406
BsmAI	GTCTC	7 cut(s)	955, 1119, 1784, 1872, 1954, 2038, 3341
BsmBI	CGTCTC	1 cut(s)	3341
BsmFI	GGGAC	3 cut(s)	199, 2106, 2553
BsmI	GAATGC	2 cut(s)	288, 315
BsnI	GGCC	5 cut(s)	267, 1489, 2942, 3187, 3241
Bso31I	GGTCTC	1 cut(s)	1872
BsoBI	CYCGRG	4 cut(s)	1781, 2087, 2132, 2300
Bsp1286I	GDGCHC	4 cut(s)	120, 2114, 3702, 3907
Bsp13I	TCCGGA	2 cut(s)	3022, 3219
Bsp1407I	TGTACA	2 cut(s)	566, 3713
Bsp19I	CCATGG	2 cut(s)	531, 3182
BspANI	GGCC	5 cut(s)	267, 1489, 2942, 3187, 3241
BspCNI	CTCAG	7 cut(s)	470, 1654, 1990, 2812, 2972, 3108, 3438
BspDI	ATCGAT	2 cut(s)	108, 1617
BspEI	TCCGGA	2 cut(s)	3022, 3219
BspHI	TCATGA	2 cut(s)	1870, 2442
BspLI	GGNNCC	5 cut(s)	868, 1637, 2145, 2358, 3108
BspMAI	CTGCAG	2 cut(s)	148, 3073
BspPI	GGATC	3 cut(s)	544, 1208, 3311
BspQI	GCTCTTC	3 cut(s)	297, 750, 758
BspT107I	GGYRCC	2 cut(s)	1635, 3106
BspTI	CTTAAG	2 cut(s)	437, 500
BspTNI	GGTCTC	1 cut(s)	1872
BsrDI	GCAATG	1 cut(s)	3832
BsrGI	TGTACA	2 cut(s)	566, 3713
BsrI	ACTGG	8 cut(s)	655, 1490, 1506, 1776, 1903, 2584, 3320, 3387
BssECI	CCNNGG	8 cut(s)	531, 1069, 1721, 1802, 2006, 2007, 3182, 3281
BssNI	GRCGYC	1 cut(s)	3735
BssT1I	CCWWGG	5 cut(s)	531, 1721, 1802, 3182, 3281
Bst2UI	CCWGG	3 cut(s)	269, 2008, 3900
Bst6I	CTCTTC	7 cut(s)	297, 693, 750, 758, 900, 1038, 1476
BstACI	GRCGYC	1 cut(s)	3735
BstAFI	CTTAAG	2 cut(s)	437, 500
BstAPI	GCANNNNNTGC	1 cut(s)	1165
BstAUI	TGTACA	2 cut(s)	566, 3713
BstBAI	YACGTR	1 cut(s)	2556
BstC8I	GCNNGC	6 cut(s)	148, 334, 632, 1332, 2857, 3708
BstDSI	CCRYGG	3 cut(s)	531, 1069, 3182
BstF5I	GGATG	8 cut(s)	73, 136, 742, 1145, 2641, 2658, 3064, 3676
BstH2I	RGCGCY	1 cut(s)	2715
BstHHI	GCGC	2 cut(s)	2227, 2714
BstMAI	GTCTC	7 cut(s)	955, 1119, 1784, 1872, 1954, 2038, 3341
BstMCI	CGRYCG	1 cut(s)	220
BstMWI	GCNNNNNNNGC	3 cut(s)	818, 1165, 3902
BstNI	CCWGG	3 cut(s)	269, 2008, 3900
BstNSI	RCATGY	2 cut(s)	1020, 3844
BstPAI	GACNNNNGTC	1 cut(s)	3734
BstSCI	CCNGG	6 cut(s)	182, 267, 1216, 2006, 2974, 3898
BstSFI	CTRYAG	4 cut(s)	144, 1699, 3069, 3793
BstSLI	GKGCMC	1 cut(s)	120
BstSNI	TACGTA	1 cut(s)	2556
BstV1I	GCAGC	7 cut(s)	1757, 1961, 2309, 2828, 3535, 3586, 3659
BstV2I	GAAGAC	3 cut(s)	629, 1593, 3859
BstX2I	RGATCY	6 cut(s)	196, 708, 1213, 1687, 2349, 3316
BstXI	CCANNNNNNTGG	2 cut(s)	2681, 2867
BstYI	RGATCY	6 cut(s)	196, 708, 1213, 1687, 2349, 3316
Bsu15I	ATCGAT	2 cut(s)	108, 1617
BsuI	GTATCC	3 cut(s)	2279, 3442, 3517
BsuRI	GGCC	5 cut(s)	267, 1489, 2942, 3187, 3241
BsuTUI	ATCGAT	2 cut(s)	108, 1617
BtgI	CCRYGG	3 cut(s)	531, 1069, 3182
BtsCI	GGATG	8 cut(s)	73, 136, 742, 1145, 2641, 2658, 3064, 3676
Cac8I	GCNNGC	6 cut(s)	148, 334, 632, 1332, 2857, 3708
CciI	TCATGA	2 cut(s)	1870, 2442
CfoI	GCGC	2 cut(s)	2227, 2714
Cfr13I	GGNCC	6 cut(s)	228, 265, 574, 867, 3047, 3562
ClaI	ATCGAT	2 cut(s)	108, 1617
CseI	GACGC	3 cut(s)	338, 623, 1083
Csp6I	GTAC	9 cut(s)	41, 567, 783, 1262, 1636, 3029, 3107, 3326, 3714
CviQI	GTAC	9 cut(s)	41, 567, 783, 1262, 1636, 3029, 3107, 3326, 3714
DraIII	CACNNNGTG	1 cut(s)	2870
DrdI	GACNNNNNNGTC	2 cut(s)	1442, 2052
DseDI	GACNNNNNNGTC	2 cut(s)	1442, 2052
Eam1104I	CTCTTC	7 cut(s)	297, 693, 750, 758, 900, 1038, 1476
EarI	CTCTTC	7 cut(s)	297, 693, 750, 758, 900, 1038, 1476
Ecl136II	GAGCTC	2 cut(s)	3700, 3905
Eco105I	TACGTA	1 cut(s)	2556
Eco130I	CCWWGG	5 cut(s)	531, 1721, 1802, 3182, 3281
Eco147I	AGGCCT	1 cut(s)	3241
Eco24I	GRGCYC	2 cut(s)	3702, 3907
Eco31I	GGTCTC	1 cut(s)	1872
Eco47I	GGWCC	5 cut(s)	228, 574, 867, 3047, 3562
Eco53kI	GAGCTC	2 cut(s)	3700, 3905
Eco57I	CTGAAG	5 cut(s)	717, 731, 862, 1643, 3228
Eco88I	CYCGRG	4 cut(s)	1781, 2087, 2132, 2300
EcoICRI	GAGCTC	2 cut(s)	3700, 3905
EcoO109I	RGGNCCY	3 cut(s)	265, 574, 867
EcoRII	CCWGG	3 cut(s)	267, 2006, 3898
EcoT14I	CCWWGG	5 cut(s)	531, 1721, 1802, 3182, 3281
EcoT22I	ATGCAT	4 cut(s)	73, 1161, 2567, 2880
EcoT38I	GRGCYC	2 cut(s)	3702, 3907
ErhI	CCWWGG	5 cut(s)	531, 1721, 1802, 3182, 3281
Esp3I	CGTCTC	1 cut(s)	3341
FalI	AAGNNNNNCTT	2 cut(s)	2399, 2431
FaqI	GGGAC	3 cut(s)	199, 2106, 2553
FauI	CCCGC	1 cut(s)	2753
FauNDI	CATATG	1 cut(s)	1551
FbaI	TGATCA	2 cut(s)	2776, 3766
FblI	GTMKAC	2 cut(s)	2056, 2552
FokI	GGATG	8 cut(s)	80, 143, 749, 1152, 2648, 2665, 3071, 3683
FriOI	GRGCYC	2 cut(s)	3702, 3907
FspBI	CTAG	8 cut(s)	476, 1424, 1454, 1538, 1967, 3237, 3596, 3623
GlaI	GCGC	2 cut(s)	2226, 2713
GsaI	CCCAGC	1 cut(s)	3130
GsuI	CTGGAG	3 cut(s)	855, 932, 3686
HaeII	RGCGCY	1 cut(s)	2715
HaeIII	GGCC	5 cut(s)	267, 1489, 2942, 3187, 3241
HapII	CCGG	5 cut(s)	183, 1218, 2975, 3023, 3220
HgaI	GACGC	3 cut(s)	338, 623, 1083
HhaI	GCGC	2 cut(s)	2227, 2714
Hin1I	GRCGYC	1 cut(s)	3735
Hin6I	GCGC	2 cut(s)	2225, 2712
HinP1I	GCGC	2 cut(s)	2225, 2712
HincII	GTYRAC	2 cut(s)	2826, 2914
HindII	GTYRAC	2 cut(s)	2826, 2914
HindIII	AAGCTT	4 cut(s)	1574, 1745, 2853, 3708
HpaI	GTTAAC	1 cut(s)	2914
HpaII	CCGG	5 cut(s)	183, 1218, 2975, 3023, 3220
HphI	GGTGA	3 cut(s)	2000, 2777, 3269
HpyCH4IV	ACGT	6 cut(s)	619, 1735, 2516, 2555, 3344, 3735
HpyF10VI	GCNNNNNNNGC	3 cut(s)	818, 1165, 3902
HpySE526I	ACGT	6 cut(s)	619, 1735, 2516, 2555, 3344, 3735
Hsp92I	GRCGYC	1 cut(s)	3735
HspAI	GCGC	2 cut(s)	2225, 2712
Kpn2I	TCCGGA	2 cut(s)	3022, 3219
KpnI	GGTACC	2 cut(s)	1639, 3110
Ksp22I	TGATCA	2 cut(s)	2776, 3766
KspAI	GTTAAC	1 cut(s)	2914
LguI	GCTCTTC	3 cut(s)	297, 750, 758
LmnI	GCTCC	8 cut(s)	951, 2143, 2330, 2356, 2749, 3025, 3705, 3902
Lsp1109I	GCAGC	7 cut(s)	1757, 1961, 2309, 2828, 3535, 3586, 3659
MaeI	CTAG	8 cut(s)	476, 1424, 1454, 1538, 1967, 3237, 3596, 3623
MaeII	ACGT	6 cut(s)	619, 1735, 2516, 2555, 3344, 3735
MaeIII	GTNAC	5 cut(s)	2483, 2684, 2698, 3076, 3275
MfeI	CAATTG	3 cut(s)	1188, 3576, 3846
MflI	RGATCY	6 cut(s)	196, 708, 1213, 1687, 2349, 3316
MhlI	GDGCHC	4 cut(s)	120, 2114, 3702, 3907
MlyI	GAGTC	7 cut(s)	1430, 1460, 1675, 2062, 2088, 2949, 3417
MmeI	TCCRAC	3 cut(s)	253, 1744, 2980
Mph1103I	ATGCAT	4 cut(s)	73, 1161, 2567, 2880
MroI	TCCGGA	2 cut(s)	3022, 3219
MslI	CAYNNNNRTG	2 cut(s)	1758, 2993
MspA1I	CMGCKG	3 cut(s)	613, 2399, 2819
MspCI	CTTAAG	2 cut(s)	437, 500
MspI	CCGG	5 cut(s)	183, 1218, 2975, 3023, 3220
MspR9I	CCNGG	6 cut(s)	184, 269, 1218, 2008, 2976, 3900
MunI	CAATTG	3 cut(s)	1188, 3576, 3846
Mva1269I	GAATGC	2 cut(s)	288, 315
MvaI	CCWGG	3 cut(s)	269, 2008, 3900
MwoI	GCNNNNNNNGC	3 cut(s)	818, 1165, 3902
NciI	CCSGG	3 cut(s)	184, 1218, 2976
NcoI	CCATGG	2 cut(s)	531, 3182
NdeI	CATATG	1 cut(s)	1551
NlaIV	GGNNCC	5 cut(s)	868, 1637, 2145, 2358, 3108
NmuCI	GTSAC	4 cut(s)	2483, 2684, 2698, 3275
NsiI	ATGCAT	4 cut(s)	73, 1161, 2567, 2880
NspI	RCATGY	2 cut(s)	1020, 3844
OliI	CACNNNNGTG	1 cut(s)	2993
PaeR7I	CTCGAG	3 cut(s)	1781, 2087, 2300
PagI	TCATGA	2 cut(s)	1870, 2442
PasI	CCCWGGG	1 cut(s)	2007
PceI	AGGCCT	1 cut(s)	3241
PciSI	GCTCTTC	3 cut(s)	297, 750, 758
PctI	GAATGC	2 cut(s)	288, 315
PfeI	GAWTC	8 cut(s)	245, 602, 660, 1505, 1856, 2659, 2723, 3173
PfoI	TCCNGGA	2 cut(s)	182, 1216
Ple19I	CGATCG	1 cut(s)	220
PleI	GAGTC	7 cut(s)	1430, 1460, 1674, 2061, 2088, 2949, 3417
PpsI	GAGTC	7 cut(s)	1430, 1460, 1674, 2061, 2088, 2949, 3417
Ppu21I	YACGTR	1 cut(s)	2556
PpuMI	RGGWCCY	2 cut(s)	574, 867
PshAI	GACNNNNGTC	1 cut(s)	3734
PsiI	TTATAA	2 cut(s)	801, 3755
Psp124BI	GAGCTC	2 cut(s)	3702, 3907
Psp1406I	AACGTT	2 cut(s)	619, 3344
Psp5II	RGGWCCY	2 cut(s)	574, 867
Psp6I	CCWGG	3 cut(s)	267, 2006, 3898
PspFI	CCCAGC	1 cut(s)	3126
PspGI	CCWGG	3 cut(s)	267, 2006, 3898
PspN4I	GGNNCC	5 cut(s)	868, 1637, 2145, 2358, 3108
PspPI	GGNCC	6 cut(s)	228, 265, 574, 867, 3047, 3562
PspPPI	RGGWCCY	2 cut(s)	574, 867
PstI	CTGCAG	2 cut(s)	148, 3073
PsuI	RGATCY	6 cut(s)	196, 708, 1213, 1687, 2349, 3316
PvuI	CGATCG	1 cut(s)	220
PvuII	CAGCTG	2 cut(s)	613, 2819
RsaI	GTAC	9 cut(s)	42, 568, 784, 1263, 1637, 3030, 3108, 3327, 3715
RsaNI	GTAC	9 cut(s)	41, 567, 783, 1262, 1636, 3029, 3107, 3326, 3714
RseI	CAYNNNNRTG	2 cut(s)	1758, 2993
SacI	GAGCTC	2 cut(s)	3702, 3907
SapI	GCTCTTC	3 cut(s)	297, 750, 758
Sau96I	GGNCC	6 cut(s)	228, 265, 574, 867, 3047, 3562
ScaI	AGTACT	1 cut(s)	784
SchI	GAGTC	7 cut(s)	1430, 1460, 1675, 2062, 2088, 2949, 3417
ScrFI	CCNGG	6 cut(s)	184, 269, 1218, 2008, 2976, 3900
SduI	GDGCHC	4 cut(s)	120, 2114, 3702, 3907
SfcI	CTRYAG	4 cut(s)	144, 1699, 3069, 3793
Sfr274I	CTCGAG	3 cut(s)	1781, 2087, 2300
SinI	GGWCC	5 cut(s)	228, 574, 867, 3047, 3562
SlaI	CTCGAG	3 cut(s)	1781, 2087, 2300
SmiMI	CAYNNNNRTG	2 cut(s)	1758, 2993
SmlI	CTYRAG	8 cut(s)	437, 500, 560, 1781, 1913, 2087, 2300, 2936
SmoI	CTYRAG	8 cut(s)	437, 500, 560, 1781, 1913, 2087, 2300, 2936
SnaBI	TACGTA	1 cut(s)	2556
SseBI	AGGCCT	1 cut(s)	3241
SspI	AATATT	3 cut(s)	1810, 1879, 2695
SspMI	CTAG	8 cut(s)	476, 1424, 1454, 1538, 1967, 3237, 3596, 3623
SstI	GAGCTC	2 cut(s)	3702, 3907
StuI	AGGCCT	1 cut(s)	3241
StyD4I	CCNGG	6 cut(s)	182, 267, 1216, 2006, 2974, 3898
StyI	CCWWGG	5 cut(s)	531, 1721, 1802, 3182, 3281
TaiI	ACGT	6 cut(s)	622, 1738, 2519, 2558, 3347, 3738
TatI	WGTACW	5 cut(s)	566, 782, 1261, 3325, 3713
TauI	GCSGC	4 cut(s)	288, 1085, 1088, 3554
TfiI	GAWTC	8 cut(s)	245, 602, 660, 1505, 1856, 2659, 2723, 3173
TseFI	GTSAC	4 cut(s)	2483, 2684, 2698, 3275
TseI	GCWGC	7 cut(s)	1770, 1974, 2322, 2816, 3548, 3599, 3647
Tsp45I	GTSAC	4 cut(s)	2483, 2684, 2698, 3275
TspGWI	ACGGA	5 cut(s)	203, 1058, 1240, 2845, 3871
Vha464I	CTTAAG	2 cut(s)	437, 500
VneI	GTGCAC	1 cut(s)	116
VpaK11BI	GGWCC	5 cut(s)	228, 574, 867, 3047, 3562
XapI	RAATTY	6 cut(s)	416, 1519, 1716, 2170, 3590, 3877
XbaI	TCTAGA	3 cut(s)	1423, 1453, 1966
XceI	RCATGY	2 cut(s)	1020, 3844
XhoI	CTCGAG	3 cut(s)	1781, 2087, 2300
XmiI	GTMKAC	2 cut(s)	2056, 2552
XspI	CTAG	8 cut(s)	476, 1424, 1454, 1538, 1967, 3237, 3596, 3623
ZraI	GACGTC	1 cut(s)	3736
ZrmI	AGTACT	1 cut(s)	784
Zsp2I	ATGCAT	4 cut(s)	73, 1161, 2567, 2880

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.