AT5G12220

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001924
Physical Location & Seq
Forward (+)
3950133 .. 3953150
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12220.1

Sequence Viewer

Length: 1836 bp
ATGGAGGCGCTAGCGGGAATTGAAGCAGACATCGAATCGTATTTCGGAGAGCAGGATCAACAGAAATCGTCACCGGACGGCTGCAAACAGGTCCCGTGGCTGAGCTGGGAAGAGTGGGACTCTGTGAGAGAGTCTCTCTTCTCTTCTTCTCCGGATAGAATCGCTTCTGCATTGGAAAGGGTTGCAACATGGCGAAGTAGAGGATCTCTTCCAGCGCCTGTGGATGTTACTTGTTCCCTTATTGAAATTCAACTCAAAGATGGCTTTATTCCGAGGGAGAAACAATCAGCAGATGCATTGTATTCAGAGCATTTACTGCAGATGCTATATTGCATGGGAATACTCAGGCTTGTGAACTGTGTCATAGAAAAGACAAGGAGGAGAGAAGACGTTTCAATTGCGGATGCTGCTAGAGCAATCGGTATCCCACGTAAATTAATCGATCTTCGTCATGAGGGTTCTCACCGTGAGCTCCCTGCACTCTCGGTGCTTCGGGATGCTGCAGATGAGGCTCTTGAATGGTTGAAATCGTATTATTGGGAGCAACAAAAGTATCAAATCCCCTTAAAGAGAGATGGAACAGCTAGCATCAGAAGAGAAGTCAAATCCAAACTCAGGAAACTTGCTTTCTCCTTGCAGCTGAAGCGTAATCCTCAATTTGACTCCCCTTTGGTCAAAGAAAAATGTTCTAATAAAAGGATAAGGAAGATTGTAAGCAGCCTTGTTGAGTTATACCCTTCTTTCTCCGCAGAAATTTCATCTGTGCTCCTTGAATTCTTACTCAAGGCCTTGGATTCTTCAAAGTCGACAGATCTTCAGAATAAGGCTGGGCAAGATTTCAGGGTCTTTCTAGATGAATGGAAACCTGTAATAATGGAACTCTCAAATAGAGAACCTGAGTTACTTTTGACTCTACTCAAGGAAGTCCTTGATATGATCCAAAATAATGAACGGAGAAGACATGAAACCGAAAAGCCAGTGGAAGAGGTTTCTCAAGTTGAGCAGGTCCCTTTCTTGTTTGCATGGCTTGTGAGTCTTCTCAATGGATCAAAGCATTTTCAGAGAAACAGTTCTCTAGAAGTGAAACCTCCAAGCATTTTCCTTATGGAACTTATACGTAGATGTCTTGTCTTGGGAGCCTTAGGATATGAGCTGGTTTTGAAATCAGCATTTCTACTCGCAGAGATTGTGGGAGGCCGTGTCTTAAAGGAGAAGCTCATAAAACTTCCTCTTATGCATAAAAGCTCTGCAATTGTTCCATTGAAACAGAGTTCTACTCTTGTAACAGCTAGTACAACGCTCCTTGAGCAAGAGAAGAATCTCGGTAATGCAGGCAAGAGACTTGAGTTCGTCAAACTTCAACTCTCAAAGAAGAAAGGTATTGACACAGACAAAACCGGTAACAGATGGAGAAAGGCAAAAACATGGAGCCCTTGTCCAATTGGTATGTTGCCTCGGATCATTGGATCTTCTGGACGTTTACCTCTTCTTGACAATCAGAATGCTAAGAGCATCTCAAAACAAGCACAAGGTAACAACAATGCGAAGAGAGGAGCTGAGTGTAATAGAAATCAATTGGAGAAATCGCCATGCAAGCGAGCAAGGAAGAGTGCAGGAGATTCTGAATCAAATGATGTGACATTGGAATCATACGAGGAAGAAGCTGAGATGGAAATAGAACATGCATATGAGGAAACTGAAACTGTAGCAGAAGAGAATCTGATGTGGAATGATGAAGAGGAAAGTAGAAGCTGCCTTATGATAGATGGTGAATGGAAAAGAGTAAATGATGGAGAACTCTCGGGGATGGCTTCTAGTGTTACAATTTGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

611

Amino Acids

69.29

Weight (kDa)

6.73

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 31 - 182 3.9e-44 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 994
AccI GTMKAC 1 cut(s) 806
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 3 cut(s) 14, 401, 747
AclWI GGATC 6 cut(s) 63, 211, 931, 1054, 1466, 1474
AcsI RAATTY 3 cut(s) 246, 753, 773
AcuI CTGAAG 2 cut(s) 662, 800
AfaI GTAC 1 cut(s) 1294
AfiI CCNNNNNNNGG 1 cut(s) 615
AgeI ACCGGT 1 cut(s) 1397
Alw21I GWGCWC 2 cut(s) 474, 768
Alw26I GTCTC 2 cut(s) 138, 1333
AlwI GGATC 6 cut(s) 63, 211, 931, 1054, 1466, 1474
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 1801
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 2 cut(s) 786, 1195
ApeKI GCWGC 6 cut(s) 81, 407, 500, 637, 717, 1752
ApoI RAATTY 3 cut(s) 246, 753, 773
AseI ATTAAT 1 cut(s) 437
AsiGI ACCGGT 1 cut(s) 1397
Asp700I GAANNNNTTC 2 cut(s) 163, 1069
AspLEI GCGC 2 cut(s) 10, 217
AspS9I GGNCC 2 cut(s) 91, 1006
AsuHPI GGTGA 3 cut(s) 63, 455, 1781
AsuNHI GCTAGC 2 cut(s) 10, 584
AvaI CYCGRG 1 cut(s) 1801
AvaII GGWCC 2 cut(s) 91, 1006
AxyI CCTNAGG 1 cut(s) 1141
BaeI ACNNNNGTAYC 2 cut(s) 536, 569
BanII GRGCYC 2 cut(s) 474, 1433
BbsI GAAGAC 3 cut(s) 393, 964, 1028
Bbv12I GWGCWC 2 cut(s) 474, 768
BbvI GCAGC 6 cut(s) 68, 394, 487, 649, 729, 1739
BccI CCATC 7 cut(s) 254, 569, 1401, 1663, 1760, 1784, 1801
BceAI ACGGC 2 cut(s) 94, 1182
BciVI GTATCC 1 cut(s) 434
BcoDI GTCTC 2 cut(s) 138, 1333
BfaI CTAG 7 cut(s) 11, 411, 585, 851, 1076, 1290, 1815
BfmI CTRYAG 3 cut(s) 317, 501, 1704
BfoI RGCGCY 2 cut(s) 11, 218
BfuAI ACCTGC 1 cut(s) 994
BfuI GTATCC 1 cut(s) 434
BglII AGATCT 1 cut(s) 811
BisI GCNGC 6 cut(s) 82, 408, 501, 638, 718, 1753
BlpI GCTNAGC 1 cut(s) 101
BlsI GCNGC 6 cut(s) 83, 409, 502, 639, 719, 1754
Bme18I GGWCC 2 cut(s) 91, 1006
BmeT110I CYCGRG 1 cut(s) 1801
BmgT120I GGNCC 2 cut(s) 91, 1006
BmiI GGNNCC 4 cut(s) 93, 1008, 1138, 1430
BmsI GCATC 6 cut(s) 283, 312, 394, 487, 597, 1521
BmtI GCTAGC 2 cut(s) 14, 588
BpiI GAAGAC 3 cut(s) 393, 964, 1028
BplI GAGNNNNNCTC 8 cut(s) 104, 136, 118, 150, 120, 152, 1261, 1293
Bpu1102I GCTNAGC 1 cut(s) 101
BpuEI CTTGAG 5 cut(s) 767, 902, 978, 1325, 1364
Bsa29I ATCGAT 1 cut(s) 441
BsaAI YACGTR 2 cut(s) 431, 1118
BsaJI CCNNGG 4 cut(s) 95, 272, 789, 1454
BsaWI WCCGGW 3 cut(s) 73, 151, 1397
BsaXI ACNNNNNCTCC 4 cut(s) 1185, 1215, 1420, 1450
Bsc4I CCNNNNNNNGG 1 cut(s) 615
Bse118I RCCGGY 1 cut(s) 1397
Bse1I ACTGG 1 cut(s) 977
Bse21I CCTNAGG 1 cut(s) 1141
BseAI TCCGGA 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 4 cut(s) 95, 272, 789, 1454
BseGI GGATG 4 cut(s) 229, 409, 502, 1812
BseLI CCNNNNNNNGG 1 cut(s) 615
BseMII CTCAG 6 cut(s) 92, 358, 628, 888, 1548, 1656
BseNI ACTGG 1 cut(s) 977
BseRI GAGGAG 2 cut(s) 394, 1566
BseXI GCAGC 6 cut(s) 68, 394, 487, 649, 729, 1739
BseYI CCCAGC 2 cut(s) 105, 827
BsgI GTGCAG 2 cut(s) 462, 1632
BshFI GGCC 2 cut(s) 788, 1197
BshTI ACCGGT 1 cut(s) 1397
BshVI ATCGAT 1 cut(s) 441
BsiHKAI GWGCWC 2 cut(s) 474, 768
BsiHKCI CYCGRG 1 cut(s) 1801
BsiSI CCGG 3 cut(s) 74, 152, 1398
BslFI GGGAC 3 cut(s) 77, 131, 992
BslI CCNNNNNNNGG 1 cut(s) 615
BsmAI GTCTC 2 cut(s) 138, 1333
BsmFI GGGAC 3 cut(s) 77, 131, 992
BsmI GAATGC 1 cut(s) 1507
BsnI GGCC 2 cut(s) 788, 1197
BsoBI CYCGRG 1 cut(s) 1801
Bsp1286I GDGCHC 3 cut(s) 474, 768, 1433
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
Bsp1720I GCTNAGC 1 cut(s) 101
BspACI CCGC 3 cut(s) 14, 401, 747
BspANI GGCC 2 cut(s) 788, 1197
BspCNI CTCAG 6 cut(s) 93, 357, 627, 889, 1549, 1657
BspDI ATCGAT 1 cut(s) 441
BspEI TCCGGA 1 cut(s) 151
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 4 cut(s) 93, 1008, 1138, 1430
BspMAI CTGCAG 2 cut(s) 321, 505
BspMI ACCTGC 1 cut(s) 994
BspOI GCTAGC 2 cut(s) 14, 588
BspPI GGATC 6 cut(s) 63, 211, 931, 1054, 1466, 1474
BsrFI RCCGGY 1 cut(s) 1397
BsrI ACTGG 1 cut(s) 977
BssAI RCCGGY 1 cut(s) 1397
BssECI CCNNGG 4 cut(s) 95, 272, 789, 1454
BssMI GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
BssT1I CCWWGG 1 cut(s) 789
Bst4CI ACNGT 4 cut(s) 359, 467, 1070, 1705
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBAI YACGTR 2 cut(s) 431, 1118
BstC8I GCNNGC 5 cut(s) 12, 586, 1333, 1595, 1599
BstDEI CTNAG 8 cut(s) 101, 344, 614, 897, 1141, 1506, 1557, 1665
BstDSI CCRYGG 1 cut(s) 95
BstF5I GGATG 4 cut(s) 229, 409, 502, 1812
BstH2I RGCGCY 2 cut(s) 11, 218
BstHHI GCGC 2 cut(s) 10, 217
BstKTI GATC 8 cut(s) 58, 206, 445, 814, 939, 1049, 1461, 1469
BstMAI GTCTC 2 cut(s) 138, 1333
BstMBI GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
BstMWI GCNNNNNNNGC 7 cut(s) 316, 407, 413, 509, 643, 1306, 1594
BstNSI RCATGY 1 cut(s) 1685
BstSFI CTRYAG 3 cut(s) 317, 501, 1704
BstSNI TACGTA 1 cut(s) 1118
BstV1I GCAGC 6 cut(s) 68, 394, 487, 649, 729, 1739
BstV2I GAAGAC 3 cut(s) 393, 964, 1028
BstX2I RGATCY 3 cut(s) 203, 811, 1466
BstYI RGATCY 3 cut(s) 203, 811, 1466
Bsu15I ATCGAT 1 cut(s) 441
Bsu36I CCTNAGG 1 cut(s) 1141
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 2 cut(s) 788, 1197
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 1 cut(s) 95
BtsCI GGATG 4 cut(s) 229, 409, 502, 1812
BtsIMutI CAGTG 1 cut(s) 984
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 5 cut(s) 12, 586, 1333, 1595, 1599
CaiI CAGNNNCTG 1 cut(s) 218
CciI TCATGA 1 cut(s) 451
CfoI GCGC 2 cut(s) 10, 217
Cfr10I RCCGGY 1 cut(s) 1397
Cfr13I GGNCC 2 cut(s) 91, 1006
ClaI ATCGAT 1 cut(s) 441
Csp6I GTAC 1 cut(s) 1293
CspAI ACCGGT 1 cut(s) 1397
CviAII CATG 8 cut(s) 189, 334, 452, 962, 1023, 1425, 1590, 1682
CviQI GTAC 1 cut(s) 1293
DdeI CTNAG 8 cut(s) 101, 344, 614, 897, 1141, 1506, 1557, 1665
DpnI GATC 8 cut(s) 57, 205, 444, 813, 938, 1048, 1460, 1468
DpnII GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
Ecl136II GAGCTC 1 cut(s) 472
Eco105I TACGTA 1 cut(s) 1118
Eco130I CCWWGG 1 cut(s) 789
Eco147I AGGCCT 1 cut(s) 788
Eco24I GRGCYC 2 cut(s) 474, 1433
Eco47I GGWCC 2 cut(s) 91, 1006
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 2 cut(s) 662, 800
Eco81I CCTNAGG 1 cut(s) 1141
Eco88I CYCGRG 1 cut(s) 1801
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 2 cut(s) 91, 1006
EcoRI GAATTC 1 cut(s) 773
EcoT14I CCWWGG 1 cut(s) 789
EcoT22I ATGCAT 3 cut(s) 298, 1239, 1687
EcoT38I GRGCYC 2 cut(s) 474, 1433
ErhI CCWWGG 1 cut(s) 789
FaeI CATG 8 cut(s) 192, 337, 455, 965, 1026, 1428, 1593, 1685
FaqI GGGAC 3 cut(s) 77, 131, 992
FatI CATG 8 cut(s) 188, 333, 451, 961, 1022, 1424, 1589, 1681
FauI CCCGC 1 cut(s) 7
FauNDI CATATG 1 cut(s) 1687
FblI GTMKAC 1 cut(s) 806
Fnu4HI GCNGC 6 cut(s) 82, 408, 501, 638, 718, 1753
FokI GGATG 4 cut(s) 236, 416, 509, 1819
FriOI GRGCYC 2 cut(s) 474, 1433
Fsp4HI GCNGC 6 cut(s) 82, 408, 501, 638, 718, 1753
FspBI CTAG 7 cut(s) 11, 411, 585, 851, 1076, 1290, 1815
GlaI GCGC 2 cut(s) 9, 216
GluI GCNGC 6 cut(s) 82, 408, 501, 638, 718, 1753
GsaI CCCAGC 2 cut(s) 109, 831
HaeII RGCGCY 2 cut(s) 11, 218
HaeIII GGCC 2 cut(s) 788, 1197
HapII CCGG 3 cut(s) 74, 152, 1398
HhaI GCGC 2 cut(s) 10, 217
Hin1II CATG 8 cut(s) 192, 337, 455, 965, 1026, 1428, 1593, 1685
Hin6I GCGC 2 cut(s) 8, 215
HinP1I GCGC 2 cut(s) 8, 215
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HpaII CCGG 3 cut(s) 74, 152, 1398
HphI GGTGA 3 cut(s) 63, 455, 1781
Hpy166II GTNNAC 3 cut(s) 355, 807, 1481
Hpy188III TCNNGA 9 cut(s) 152, 452, 494, 515, 616, 851, 1076, 1473, 1490
Hpy8I GTNNAC 3 cut(s) 355, 807, 1481
HpyAV CCTTC 1 cut(s) 747
HpyCH4III ACNGT 4 cut(s) 359, 467, 1070, 1705
HpyCH4IV ACGT 4 cut(s) 390, 430, 1117, 1477
HpyF10VI GCNNNNNNNGC 7 cut(s) 316, 407, 413, 509, 643, 1306, 1594
HpyF3I CTNAG 8 cut(s) 101, 344, 614, 897, 1141, 1506, 1557, 1665
HpySE526I ACGT 4 cut(s) 390, 430, 1117, 1477
Hsp92II CATG 8 cut(s) 192, 337, 455, 965, 1026, 1428, 1593, 1685
HspAI GCGC 2 cut(s) 8, 215
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
LmnI GCTCC 7 cut(s) 477, 541, 771, 1136, 1305, 1428, 1553
Lsp1109I GCAGC 6 cut(s) 68, 394, 487, 649, 729, 1739
LweI GCATC 6 cut(s) 283, 312, 394, 487, 597, 1521
MaeI CTAG 7 cut(s) 11, 411, 585, 851, 1076, 1290, 1815
MaeII ACGT 4 cut(s) 390, 430, 1117, 1477
MaeIII GTNAC 8 cut(s) 69, 226, 900, 1282, 1400, 1532, 1636, 1819
MalI GATC 8 cut(s) 57, 205, 444, 813, 938, 1048, 1460, 1468
MboI GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
MfeI CAATTG 4 cut(s) 396, 1251, 1440, 1574
MflI RGATCY 3 cut(s) 203, 811, 1466
MhlI GDGCHC 3 cut(s) 474, 768, 1433
MlyI GAGTC 5 cut(s) 113, 140, 656, 904, 1042
Mph1103I ATGCAT 3 cut(s) 298, 1239, 1687
MroI TCCGGA 1 cut(s) 151
MroXI GAANNNNTTC 2 cut(s) 163, 1069
MseI TTAA 3 cut(s) 437, 566, 1205
MslI CAYNNNNRTG 1 cut(s) 1686
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 3 cut(s) 74, 152, 1398
MunI CAATTG 4 cut(s) 396, 1251, 1440, 1574
Mva1269I GAATGC 1 cut(s) 1507
MwoI GCNNNNNNNGC 7 cut(s) 316, 407, 413, 509, 643, 1306, 1594
NdeI CATATG 1 cut(s) 1687
NdeII GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
NheI GCTAGC 2 cut(s) 10, 584
NlaIII CATG 8 cut(s) 192, 337, 455, 965, 1026, 1428, 1593, 1685
NlaIV GGNNCC 4 cut(s) 93, 1008, 1138, 1430
NmuCI GTSAC 2 cut(s) 69, 1636
NsiI ATGCAT 3 cut(s) 298, 1239, 1687
NspI RCATGY 1 cut(s) 1685
PagI TCATGA 1 cut(s) 451
PceI AGGCCT 1 cut(s) 788
PctI GAATGC 1 cut(s) 1507
PdmI GAANNNNTTC 2 cut(s) 163, 1069
PfeI GAWTC 8 cut(s) 35, 159, 794, 1318, 1619, 1625, 1646, 1717
PinAI ACCGGT 1 cut(s) 1397
PkrI GCNGC 6 cut(s) 83, 409, 502, 639, 719, 1754
PleI GAGTC 5 cut(s) 113, 139, 656, 904, 1041
PpsI GAGTC 5 cut(s) 113, 139, 656, 904, 1041
Ppu21I YACGTR 2 cut(s) 431, 1118
PpuMI RGGWCCY 2 cut(s) 91, 1006
PshBI ATTAAT 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 474
Psp5II RGGWCCY 2 cut(s) 91, 1006
PspFI CCCAGC 2 cut(s) 105, 827
PspN4I GGNNCC 4 cut(s) 93, 1008, 1138, 1430
PspPI GGNCC 2 cut(s) 91, 1006
PspPPI RGGWCCY 2 cut(s) 91, 1006
PsrI GAACNNNNNNTAC 2 cut(s) 885, 917
PstI CTGCAG 2 cut(s) 321, 505
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 203, 811, 1466
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 1 cut(s) 1294
RsaNI GTAC 1 cut(s) 1293
RseI CAYNNNNRTG 1 cut(s) 1686
SacI GAGCTC 1 cut(s) 474
SalI GTCGAC 1 cut(s) 805
SaqAI TTAA 3 cut(s) 437, 566, 1205
SatI GCNGC 6 cut(s) 82, 408, 501, 638, 718, 1753
Sau3AI GATC 8 cut(s) 55, 203, 442, 811, 936, 1046, 1458, 1466
Sau96I GGNCC 2 cut(s) 91, 1006
SchI GAGTC 5 cut(s) 113, 140, 656, 904, 1042
SduI GDGCHC 3 cut(s) 474, 768, 1433
SfaNI GCATC 6 cut(s) 283, 312, 394, 487, 597, 1521
SfcI CTRYAG 3 cut(s) 317, 501, 1704
SinI GGWCC 2 cut(s) 91, 1006
SmiMI CAYNNNNRTG 1 cut(s) 1686
SmlI CTYRAG 5 cut(s) 782, 917, 993, 1304, 1343
SmoI CTYRAG 5 cut(s) 782, 917, 993, 1304, 1343
SnaBI TACGTA 1 cut(s) 1118
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 3 cut(s) 14, 401, 747
SspMI CTAG 7 cut(s) 11, 411, 585, 851, 1076, 1290, 1815
SstI GAGCTC 1 cut(s) 474
StuI AGGCCT 1 cut(s) 788
StyI CCWWGG 1 cut(s) 789
TaaI ACNGT 4 cut(s) 359, 467, 1070, 1705
TaiI ACGT 4 cut(s) 393, 433, 1120, 1480
TaqI TCGA 3 cut(s) 33, 441, 806
TatI WGTACW 1 cut(s) 1292
TfiI GAWTC 8 cut(s) 35, 159, 794, 1318, 1619, 1625, 1646, 1717
Tru1I TTAA 3 cut(s) 437, 566, 1205
Tru9I TTAA 3 cut(s) 437, 566, 1205
TscAI CASTG 1 cut(s) 984
TseFI GTSAC 2 cut(s) 69, 1636
TseI GCWGC 6 cut(s) 81, 407, 500, 637, 717, 1752
Tsp45I GTSAC 2 cut(s) 69, 1636
TspDTI ATGAA 5 cut(s) 747, 870, 963, 978, 1749
TspGWI ACGGA 1 cut(s) 967
TspRI CASTG 1 cut(s) 984
VpaK11BI GGWCC 2 cut(s) 91, 1006
VspI ATTAAT 1 cut(s) 437
XapI RAATTY 3 cut(s) 246, 753, 773
XbaI TCTAGA 2 cut(s) 850, 1075
XceI RCATGY 1 cut(s) 1685
XmiI GTMKAC 1 cut(s) 806
XmnI GAANNNNTTC 2 cut(s) 163, 1069
XspI CTAG 7 cut(s) 11, 411, 585, 851, 1076, 1290, 1815
Zsp2I ATGCAT 3 cut(s) 298, 1239, 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.