Rh7CG342000

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001924
Physical Location & Seq
Forward (+)
38395556 .. 38398410
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG342000.1

Sequence Viewer

Length: 516 bp
ATGGAAGAACCGGGGGATTATGACCAGATATCGGCATGGAGAAGCAGAGGATGTGTTCCTGCTGTGATCGAAGTTACAGCTTCAATAATTGAAATTCAGCAAAAAGATCCTCACTTTAGAAAGGACCAGTCAAATGATGCTGTGGATAATTGCCAAGCAGACCAATCCAGTGATTCTTCACTTTCTAAGGAAATGCTGGCCATGCTATATTGCATGGCAATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGGTTTCAATTGCTGAGGCTGCTGATGCATTTGGCATTCCACGATCAGTGATTGATATTCGTCATGAGGGATCTCACCGTGAGCTTCCCGCTCTTCAGGTTGTTCGAAGCGCTTCAATTAAGGCCCTTGATTGGTTGAAGTATTATTATTGGGAACCTCAGAAGAATGCAATTCCATTTCAAGGCACTGGAAGTGCTAACATCAGAAATGAAATAAAGTCAAAGTTCCGTGAATTGGCTTCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

171

Amino Acids

19.28

Weight (kDa)

5.95

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 8 - 142 5.7e-35 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 359
AciI CCGC 1 cut(s) 357
AclWI GGATC 2 cut(s) 101, 346
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 347
AfeI AGCGCT 1 cut(s) 379
AfiI CCNNNNNNNGG 4 cut(s) 31, 399, 449, 502
AgsI TTSAA 6 cut(s) 84, 92, 276, 384, 406, 449
AluBI AGCT 3 cut(s) 80, 352, 513
AluI AGCT 3 cut(s) 80, 352, 513
AlwI GGATC 2 cut(s) 101, 346
Aor51HI AGCGCT 1 cut(s) 379
AoxI GGCC 2 cut(s) 198, 390
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 93
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 124, 391
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 335
AsuII TTCGAA 1 cut(s) 373
AvaII GGWCC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 257
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 274
BcnI CCSGG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 381
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 2 cut(s) 124, 391
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 2 cut(s) 127, 283
BpiI GAAGAC 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 399, 449, 502
Bse1I ACTGG 3 cut(s) 127, 168, 460
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 4 cut(s) 31, 399, 449, 502
BseMII CTCAG 2 cut(s) 273, 440
BseNI ACTGG 3 cut(s) 127, 168, 460
BseXI GCAGC 1 cut(s) 274
BshFI GGCC 2 cut(s) 200, 392
BsiSI CCGG 1 cut(s) 11
BslI CCNNNNNNNGG 4 cut(s) 31, 399, 449, 502
BsmI GAATGC 2 cut(s) 303, 439
BsnI GGCC 2 cut(s) 200, 392
Bsp119I TTCGAA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 66, 106, 311, 338
BspACI CCGC 1 cut(s) 357
BspANI GGCC 2 cut(s) 200, 392
BspCNI CTCAG 2 cut(s) 274, 439
BspHI TCATGA 2 cut(s) 222, 331
BspLI GGNNCC 1 cut(s) 423
BspPI GGATC 2 cut(s) 101, 346
BspQI GCTCTTC 1 cut(s) 366
BspT104I TTCGAA 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 359
BsrI ACTGG 3 cut(s) 127, 168, 460
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 4 cut(s) 66, 106, 311, 338
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 366
BstBI TTCGAA 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 186, 282, 426
BstF5I GGATG 1 cut(s) 56
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 4 cut(s) 69, 109, 314, 341
BstMBI GATC 4 cut(s) 66, 106, 311, 338
BstMWI GCNNNNNNNGC 3 cut(s) 202, 287, 293
BstSCI CCNGG 1 cut(s) 10
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 2 cut(s) 106, 338
BstYI RGATCY 2 cut(s) 106, 338
BsuRI GGCC 2 cut(s) 200, 392
BtsCI GGATG 1 cut(s) 56
BtsIMutI CAGTG 3 cut(s) 175, 321, 453
Cac8I GCNNGC 1 cut(s) 198
CciI TCATGA 2 cut(s) 222, 331
CfoI GCGC 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 124, 391
CviAII CATG 5 cut(s) 36, 202, 214, 223, 332
CviJI RGCY 8 cut(s) 80, 200, 229, 287, 352, 392, 506, 513
CviKI_1 RGCY 8 cut(s) 80, 200, 229, 287, 352, 392, 506, 513
DdeI CTNAG 3 cut(s) 186, 282, 426
DpnI GATC 4 cut(s) 68, 108, 313, 340
DpnII GATC 4 cut(s) 66, 106, 311, 338
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 124
Eco47III AGCGCT 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 391
EcoRV GATATC 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 298
FaeI CATG 5 cut(s) 39, 205, 217, 226, 335
FaiI YATR 7 cut(s) 21, 37, 203, 208, 215, 224, 333
FatI CATG 5 cut(s) 35, 201, 213, 222, 331
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 1 cut(s) 379
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 2 cut(s) 200, 392
HapII CCGG 1 cut(s) 11
HhaI GCGC 1 cut(s) 380
Hin1II CATG 5 cut(s) 39, 205, 217, 226, 335
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 335
Hpy188I TCNGA 2 cut(s) 429, 473
Hpy188III TCNNGA 2 cut(s) 223, 332
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4V TGCA 3 cut(s) 213, 296, 437
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 287, 293
HpyF3I CTNAG 3 cut(s) 186, 282, 426
Hsp92II CATG 5 cut(s) 39, 205, 217, 226, 335
HspAI GCGC 1 cut(s) 378
Kzo9I GATC 4 cut(s) 66, 106, 311, 338
LguI GCTCTTC 1 cut(s) 366
LpnPI CCDG 8 cut(s) 24, 38, 72, 140, 181, 182, 350, 441
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 2 cut(s) 127, 283
MaeIII GTNAC 1 cut(s) 73
MalI GATC 4 cut(s) 68, 108, 313, 340
MbiI CCGCTC 1 cut(s) 359
MboI GATC 4 cut(s) 66, 106, 311, 338
MboII GAAGA 5 cut(s) 17, 168, 262, 353, 442
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 2 cut(s) 106, 338
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 8 cut(s) 87, 93, 148, 235, 276, 384, 438, 500
MluNI TGGCCA 1 cut(s) 200
MnlI CCTC 7 cut(s) 41, 120, 219, 262, 277, 328, 435
Mox20I TGGCCA 1 cut(s) 200
Mph1103I ATGCAT 1 cut(s) 298
MroXI GAANNNNTTC 1 cut(s) 379
MscI TGGCCA 1 cut(s) 200
MseI TTAA 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 12
MunI CAATTG 1 cut(s) 276
Mva1269I GAATGC 2 cut(s) 303, 439
MwoI GCNNNNNNNGC 3 cut(s) 202, 287, 293
NciI CCSGG 1 cut(s) 12
NdeII GATC 4 cut(s) 66, 106, 311, 338
NlaIII CATG 5 cut(s) 39, 205, 217, 226, 335
NlaIV GGNNCC 1 cut(s) 423
NsiI ATGCAT 1 cut(s) 298
NspV TTCGAA 1 cut(s) 373
PagI TCATGA 2 cut(s) 222, 331
PciSI GCTCTTC 1 cut(s) 366
PctI GAATGC 2 cut(s) 303, 439
PdmI GAANNNNTTC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 2 cut(s) 124, 391
PsuI RGATCY 2 cut(s) 106, 338
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 4 cut(s) 66, 106, 311, 338
Sau96I GGNCC 2 cut(s) 124, 391
ScrFI CCNGG 1 cut(s) 12
SetI ASST 6 cut(s) 82, 273, 354, 369, 427, 515
SfaNI GCATC 2 cut(s) 127, 283
SfuI TTCGAA 1 cut(s) 373
SinI GGWCC 1 cut(s) 124
Sse9I AATT 8 cut(s) 87, 93, 148, 235, 276, 384, 438, 500
SsiI CCGC 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 10
TaaI ACNGT 1 cut(s) 347
TaqI TCGA 2 cut(s) 69, 373
TasI AATT 8 cut(s) 87, 93, 148, 235, 276, 384, 438, 500
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 3 cut(s) 175, 321, 460
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 492
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 3 cut(s) 175, 321, 460
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.