Prupe.4G267500_v2.0.a1

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001924
Physical Location & Seq
Reverse (-)
20111924 .. 20120220
8297 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G267500.2

Sequence Viewer

Length: 2013 bp
ATGGAGGCACTTTTAGGGTTTAAGGAAGAATCAACAGATTATGACCAACTTGTGATATCAAAGCCGTCGTCATCAGCATCCTACAGCTACAAATTAGTGCCGTGGTTGAGCTGGGACGAGTGGCTTTTCGTCGACGAATCTCTCTTTTCGATTTCACCTGAATCTGTGGCCTCCGCTTTAAGAAGAATATCAGCATGGAGAAGCAGAGGTTGTCTTCCTGTTGTAATTGAAGTTACGGCATCGATTATTGAAATTCATCAGAAAGATCCTCACTTTAGAAAGGACCAGTCAAATGATGCTTTGGATAATTGCAGAGCAGATCAATCAAGTGATGCTTCACTTTCAGATGAAATGCTGGCCATGTTATATTGTATGGCAATCATGAGGCTTGTGAATGGTGTTGTTGAGAAGACACGGAAGAAAACAGAGGTTTCAATTGCTGTGGCTGCTGATGCAATTGGCATTCCACGTACACTGATTGATATTCGTCATGAGGGATCTCACCGTGAACTTCCCGCACTGGAGGTTGTTCGTAGTGCTTCAGTTAAGGCCCTTGATTGGTTGAAGTACTACTACTGGGAGCCTCAGAAGAAGGCAATTCCATTTCATGGTAATGAGACTGCTGACATCAGAACTGAGATAAGGTCGAAATTCCATGAATTGGCTTTCTGCTTAAGAGTTAAGAACACTCCTCCGTCGGTTTCTTCGAAAGTCAAGGAAAAACGTGGTTGCAGAAGTAGTAATTGGCTTTGTGGGCGTAATAAGTTTTTCTCACTAATGGCTGGCAAGATCCATTCATCAAAATCTAGACGTTCCAAGAAAGACCTTACCAAGATATTGAAAAGTCTTGTAGGGTTGTATGCTTCCTTCTCCTCAGAAGTTGTTTCTGTACTGTTGGAGATCTTGTTGAATGCTATAAGTATCTCAGATTCCTCGAAGCTTCCAGTAAACACCCAAAAAGGCCCGAGCCTACATATTTCATTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGGAACCAGAGTTACTTCTGGCTCTCCTGAATGCAGCTCTTGATATGATTGAGACTCAGGAAGCAGTGCTATTTGAGACAGGAGGGGTTCGCACATCATCAGACCATAGGGCAGAGATACTCCAAGTTGAACACCTCTCTTCTCTGTTTAAGTGGCTAGTTGGGAATTTTGAAGGATTAAAGCCTCATCTTGAAAAATATTCTGCACCTGAGATCAAAGTTTCTTCAGGAGAAAAAACGATATCGAAGGCAATTCTGATGGAACTCCTACGTAAATGCCTTGTTCTATCAGCTTCTGCTAATAACCAGCTCATGGATTCTGCCGTCCATCTTGCACAACTGACTGGGGACAACTACCTATTGGGAAAACTGAACAAACTCTCTTCATTAGTTTCATCAAATTTGGATGTTACTGAAGAAGATAATTCTCTTAAACATTCAACTAATGTATTTAAGAACGAAGACGAATCTATAAGTGAAGCATCAAAGAAGCTAGAGATGATTAAACAGCGCAAAATGAAGAGCAAAGTAGTGAAGGGAACAGATGGTGATGCCGGAAATACTAACAGATGGGTTCTGGCAAAATCATGGAACCCGTGTCCAATTGGAATGTTGCCTCGAGCTGTTGGGTCTTCTGGTTGCCTTCCCGTTCTGGACTTCGATACTGTCCCAGAAAAGGTTTCTCAGTTACTAGGAAGAAAAGAGAACTGGGAATTGAACCAGTGTAGTGGCAAGAGAGAAGCTAGTTCCGATATACAGCTACTAGATAACCGATGTGTTAAGAAGTTGAGGGAAACAGAGGATGGCTGTGTATCAGATAGTGAGCATGTTTTATCCACAGAAGATGTCAGTGGTCATCTCATGATAGGCGGGGTCTGGAAGAAAGTTGGGGAAGAAGAGCTACTTGCCATCAAGTCTGCTGTAAGGATTTGGCCACACACAAAAAAGCCTGCTGTAAAAATTGAGCCACACAAAAAAAAAAGCCTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

671

Amino Acids

74.77

Weight (kDa)

8.67

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 608, 661, 1333
AccI GTMKAC 1 cut(s) 132
AciI CCGC 3 cut(s) 174, 516, 1890
AclWI GGATC 3 cut(s) 260, 505, 784
AcoI YGGCCR 2 cut(s) 357, 1952
AcsI RAATTY 5 cut(s) 252, 650, 1010, 1186, 1420
AcuI CTGAAG 3 cut(s) 525, 1230, 1455
AfaI GTAC 3 cut(s) 472, 569, 891
AfiI CCNNNNNNNGG 5 cut(s) 558, 608, 661, 1333, 1696
AflII CTTAAG 1 cut(s) 673
AjuI GAANNNNNNNTTGG 2 cut(s) 727, 759
Alw26I GTCTC 3 cut(s) 611, 1067, 1091
AlwI GGATC 3 cut(s) 260, 505, 784
AlwNI CAGNNNCTG 1 cut(s) 1316
Ama87I CYCGRG 2 cut(s) 964, 1638
AoxI GGCC 5 cut(s) 168, 357, 549, 961, 1952
ApeKI GCWGC 2 cut(s) 446, 1055
ApoI RAATTY 5 cut(s) 252, 650, 1010, 1186, 1420
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 1 cut(s) 1533
AspS9I GGNCC 3 cut(s) 283, 550, 962
AsuHPI GGTGA 3 cut(s) 147, 494, 1580
AsuII TTCGAA 1 cut(s) 707
AvaI CYCGRG 2 cut(s) 964, 1638
AvaII GGWCC 1 cut(s) 283
BalI TGGCCA 2 cut(s) 359, 1954
BarI GAAGNNNNNNTAC 6 cut(s) 557, 589, 851, 883, 1905, 1937
BbsI GAAGAC 4 cut(s) 206, 416, 1488, 1644
BbvI GCAGC 2 cut(s) 433, 1067
BccI CCATC 6 cut(s) 1273, 1356, 1559, 1584, 1817, 1937
BceAI ACGGC 4 cut(s) 49, 85, 252, 1328
BcoDI GTCTC 3 cut(s) 611, 1067, 1091
BfaI CTAG 6 cut(s) 807, 1178, 1514, 1712, 1764, 1784
BfmI CTRYAG 1 cut(s) 82
BfrI CTTAAG 1 cut(s) 673
BglII AGATCT 1 cut(s) 900
BisI GCNGC 2 cut(s) 447, 1056
BlsI GCNGC 2 cut(s) 448, 1057
BmcAI AGTACT 1 cut(s) 569
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 2 cut(s) 964, 1638
BmgT120I GGNCC 3 cut(s) 283, 550, 962
BmiI GGNNCC 3 cut(s) 582, 1026, 1613
BmrI ACTGGG 3 cut(s) 586, 1374, 1738
BmsI GCATC 7 cut(s) 86, 248, 286, 322, 442, 1511, 1561
BmuI ACTGGG 3 cut(s) 586, 1374, 1738
BpiI GAAGAC 4 cut(s) 206, 416, 1488, 1644
BpmI CTGGAG 1 cut(s) 542
Bpu14I TTCGAA 1 cut(s) 707
Bsa29I ATCGAT 1 cut(s) 242
BsaAI YACGTR 2 cut(s) 470, 1292
BsaJI CCNNGG 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 5 cut(s) 558, 608, 661, 1333, 1696
Bse1I ACTGG 7 cut(s) 286, 525, 581, 944, 1369, 1733, 1741
BseCI ATCGAT 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 3 cut(s) 77, 1432, 1828
BseLI CCNNNNNNNGG 5 cut(s) 558, 608, 661, 1333, 1696
BseMII CTCAG 7 cut(s) 599, 627, 888, 939, 1091, 1221, 1718
BseNI ACTGG 7 cut(s) 286, 525, 581, 944, 1369, 1733, 1741
BseRI GAGGAG 2 cut(s) 681, 862
BseXI GCAGC 2 cut(s) 433, 1067
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 5 cut(s) 170, 359, 551, 963, 1954
BshVI ATCGAT 1 cut(s) 242
BsiHKCI CYCGRG 2 cut(s) 964, 1638
BsiSI CCGG 1 cut(s) 1575
BslFI GGGAC 3 cut(s) 128, 1382, 1673
BslI CCNNNNNNNGG 5 cut(s) 558, 608, 661, 1333, 1696
BsmAI GTCTC 3 cut(s) 611, 1067, 1091
BsmFI GGGAC 3 cut(s) 128, 1382, 1673
BsmI GAATGC 3 cut(s) 462, 916, 1057
BsnI GGCC 5 cut(s) 170, 359, 551, 963, 1954
BsoBI CYCGRG 2 cut(s) 964, 1638
Bsp119I TTCGAA 1 cut(s) 707
Bsp143I GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
BspACI CCGC 3 cut(s) 174, 516, 1890
BspANI GGCC 5 cut(s) 170, 359, 551, 963, 1954
BspCNI CTCAG 7 cut(s) 598, 628, 887, 938, 1090, 1222, 1717
BspDI ATCGAT 1 cut(s) 242
BspHI TCATGA 3 cut(s) 381, 490, 1881
BspLI GGNNCC 3 cut(s) 582, 1026, 1613
BspPI GGATC 3 cut(s) 260, 505, 784
BspQI GCTCTTC 2 cut(s) 1535, 1911
BspT104I TTCGAA 1 cut(s) 707
BspTI CTTAAG 1 cut(s) 673
BsrI ACTGG 7 cut(s) 286, 525, 581, 944, 1369, 1733, 1741
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
Bst4CI ACNGT 3 cut(s) 506, 894, 1687
Bst6I CTCTTC 4 cut(s) 1165, 1408, 1535, 1911
BstAFI CTTAAG 1 cut(s) 673
BstBAI YACGTR 2 cut(s) 470, 1292
BstBI TTCGAA 1 cut(s) 707
BstC8I GCNNGC 4 cut(s) 357, 784, 1971, 2006
BstDEI CTNAG 7 cut(s) 585, 636, 874, 925, 1077, 1230, 1704
BstDSI CCRYGG 1 cut(s) 101
BstF5I GGATG 3 cut(s) 77, 1432, 1828
BstHHI GCGC 1 cut(s) 1533
BstKTI GATC 6 cut(s) 268, 322, 500, 792, 903, 1236
BstMAI GTCTC 3 cut(s) 611, 1067, 1091
BstMBI GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
BstMWI GCNNNNNNNGC 3 cut(s) 446, 452, 754
BstNSI RCATGY 1 cut(s) 1850
BstSFI CTRYAG 1 cut(s) 82
BstSNI TACGTA 1 cut(s) 1292
BstV1I GCAGC 2 cut(s) 433, 1067
BstV2I GAAGAC 4 cut(s) 206, 416, 1488, 1644
BstX2I RGATCY 4 cut(s) 265, 497, 789, 900
BstXI CCANNNNNNTGG 1 cut(s) 1748
BstYI RGATCY 4 cut(s) 265, 497, 789, 900
Bsu15I ATCGAT 1 cut(s) 242
BsuRI GGCC 5 cut(s) 170, 359, 551, 963, 1954
BsuTUI ATCGAT 1 cut(s) 242
BtgI CCRYGG 1 cut(s) 101
BtsCI GGATG 3 cut(s) 77, 1432, 1828
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 5 cut(s) 473, 518, 1092, 1748, 1876
Cac8I GCNNGC 4 cut(s) 357, 784, 1971, 2006
CaiI CAGNNNCTG 1 cut(s) 1316
CciI TCATGA 3 cut(s) 381, 490, 1881
CfoI GCGC 1 cut(s) 1533
Cfr13I GGNCC 3 cut(s) 283, 550, 962
ClaI ATCGAT 1 cut(s) 242
Csp6I GTAC 3 cut(s) 471, 568, 890
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviQI GTAC 3 cut(s) 471, 568, 890
DdeI CTNAG 7 cut(s) 585, 636, 874, 925, 1077, 1230, 1704
DpnI GATC 6 cut(s) 267, 321, 499, 791, 902, 1235
DpnII GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
EaeI YGGCCR 2 cut(s) 357, 1952
Eam1104I CTCTTC 4 cut(s) 1165, 1408, 1535, 1911
EarI CTCTTC 4 cut(s) 1165, 1408, 1535, 1911
Eco105I TACGTA 1 cut(s) 1292
Eco32I GATATC 2 cut(s) 57, 1263
Eco47I GGWCC 1 cut(s) 283
Eco57I CTGAAG 3 cut(s) 525, 1230, 1455
Eco88I CYCGRG 2 cut(s) 964, 1638
EcoO109I RGGNCCY 1 cut(s) 550
EcoRV GATATC 2 cut(s) 57, 1263
FalI AAGNNNNNCTT 4 cut(s) 319, 351, 1908, 1940
FaqI GGGAC 3 cut(s) 128, 1382, 1673
FauI CCCGC 2 cut(s) 523, 1883
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 447, 1056
FokI GGATG 3 cut(s) 64, 1439, 1835
Fsp4HI GCNGC 2 cut(s) 447, 1056
FspBI CTAG 6 cut(s) 807, 1178, 1514, 1712, 1764, 1784
GlaI GCGC 1 cut(s) 1532
GluI GCNGC 2 cut(s) 447, 1056
GsaI CCCAGC 1 cut(s) 115
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 5 cut(s) 170, 359, 551, 963, 1954
HapII CCGG 1 cut(s) 1575
HhaI GCGC 1 cut(s) 1533
Hin6I GCGC 1 cut(s) 1531
HinP1I GCGC 1 cut(s) 1531
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 938, 995
HinfI GANTC 7 cut(s) 29, 137, 161, 929, 1075, 1337, 1487
HpaII CCGG 1 cut(s) 1575
HphI GGTGA 3 cut(s) 147, 494, 1580
Hpy166II GTNNAC 4 cut(s) 133, 473, 509, 949
Hpy8I GTNNAC 4 cut(s) 133, 473, 509, 949
Hpy99I CGWCG 4 cut(s) 70, 134, 137, 700
HpyAV CCTTC 6 cut(s) 586, 877, 1187, 1261, 1549, 1673
HpyCH4III ACNGT 3 cut(s) 506, 894, 1687
HpyCH4IV ACGT 4 cut(s) 469, 724, 811, 1291
HpyCH4V TGCA 6 cut(s) 312, 455, 732, 1055, 1226, 1355
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 452, 754
HpyF3I CTNAG 7 cut(s) 585, 636, 874, 925, 1077, 1230, 1704
HpySE526I ACGT 4 cut(s) 469, 724, 811, 1291
HspAI GCGC 1 cut(s) 1531
Kzo9I GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
LguI GCTCTTC 2 cut(s) 1535, 1911
LmnI GCTCC 1 cut(s) 580
Lsp1109I GCAGC 2 cut(s) 433, 1067
LweI GCATC 7 cut(s) 86, 248, 286, 322, 442, 1511, 1561
MaeI CTAG 6 cut(s) 807, 1178, 1514, 1712, 1764, 1784
MaeII ACGT 4 cut(s) 469, 724, 811, 1291
MaeIII GTNAC 4 cut(s) 232, 1032, 1429, 1707
MalI GATC 6 cut(s) 267, 321, 499, 791, 902, 1235
MboI GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
MfeI CAATTG 3 cut(s) 435, 456, 1623
MflI RGATCY 4 cut(s) 265, 497, 789, 900
MlsI TGGCCA 2 cut(s) 359, 1954
MluNI TGGCCA 2 cut(s) 359, 1954
MlyI GAGTC 1 cut(s) 1069
MmeI TCCRAC 1 cut(s) 876
Mox20I TGGCCA 2 cut(s) 359, 1954
MscI TGGCCA 2 cut(s) 359, 1954
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 2 cut(s) 359, 1954
MspCI CTTAAG 1 cut(s) 673
MspI CCGG 1 cut(s) 1575
MunI CAATTG 3 cut(s) 435, 456, 1623
Mva1269I GAATGC 3 cut(s) 462, 916, 1057
MwoI GCNNNNNNNGC 3 cut(s) 446, 452, 754
NdeII GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
NlaIV GGNNCC 3 cut(s) 582, 1026, 1613
NspI RCATGY 1 cut(s) 1850
NspV TTCGAA 1 cut(s) 707
PaeR7I CTCGAG 1 cut(s) 1638
PagI TCATGA 3 cut(s) 381, 490, 1881
PciSI GCTCTTC 2 cut(s) 1535, 1911
PcsI WCGNNNNNNNCGW 1 cut(s) 704
PctI GAATGC 3 cut(s) 462, 916, 1057
PfeI GAWTC 6 cut(s) 29, 137, 161, 929, 1337, 1487
PflMI CCANNNNNTGG 3 cut(s) 608, 661, 1333
PkrI GCNGC 2 cut(s) 448, 1057
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
Ppu21I YACGTR 2 cut(s) 470, 1292
PspFI CCCAGC 1 cut(s) 111
PspN4I GGNNCC 3 cut(s) 582, 1026, 1613
PspPI GGNCC 3 cut(s) 283, 550, 962
PspXI VCTCGAGB 1 cut(s) 1638
PsrI GAACNNNNNNTAC 2 cut(s) 1017, 1049
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 4 cut(s) 265, 497, 789, 900
RsaI GTAC 3 cut(s) 472, 569, 891
RsaNI GTAC 3 cut(s) 471, 568, 890
RseI CAYNNNNRTG 1 cut(s) 612
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 2 cut(s) 1535, 1911
SatI GCNGC 2 cut(s) 447, 1056
Sau3AI GATC 6 cut(s) 265, 319, 497, 789, 900, 1233
Sau96I GGNCC 3 cut(s) 283, 550, 962
ScaI AGTACT 1 cut(s) 569
SchI GAGTC 1 cut(s) 1069
SfaNI GCATC 7 cut(s) 86, 248, 286, 322, 442, 1511, 1561
SfcI CTRYAG 1 cut(s) 82
Sfr274I CTCGAG 1 cut(s) 1638
SfuI TTCGAA 1 cut(s) 707
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 1 cut(s) 283
SlaI CTCGAG 1 cut(s) 1638
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 673, 1638
SmoI CTYRAG 2 cut(s) 673, 1638
SnaBI TACGTA 1 cut(s) 1292
SsiI CCGC 3 cut(s) 174, 516, 1890
SspI AATATT 1 cut(s) 1220
SspMI CTAG 6 cut(s) 807, 1178, 1514, 1712, 1764, 1784
TaaI ACNGT 3 cut(s) 506, 894, 1687
TaiI ACGT 4 cut(s) 472, 727, 814, 1294
TaqI TCGA 9 cut(s) 132, 149, 242, 647, 707, 935, 1265, 1639, 1680
TatI WGTACW 2 cut(s) 567, 889
TfiI GAWTC 6 cut(s) 29, 137, 161, 929, 1337, 1487
TscAI CASTG 5 cut(s) 480, 525, 1092, 1748, 1876
TseI GCWGC 2 cut(s) 446, 1055
TspDTI ATGAA 9 cut(s) 245, 363, 596, 672, 786, 969, 1395, 1404, 1553
TspGWI ACGGA 2 cut(s) 430, 684
TspRI CASTG 5 cut(s) 480, 525, 1092, 1748, 1876
Van91I CCANNNNNTGG 3 cut(s) 608, 661, 1333
Vha464I CTTAAG 1 cut(s) 673
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 5 cut(s) 252, 650, 1010, 1186, 1420
XbaI TCTAGA 1 cut(s) 806
XceI RCATGY 1 cut(s) 1850
XhoI CTCGAG 1 cut(s) 1638
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 6 cut(s) 807, 1178, 1514, 1712, 1764, 1784
ZrmI AGTACT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.