Rh7CG381700

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001924
Physical Location & Seq
Forward (+)
48101075 .. 48110907
9833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG381700.1

Sequence Viewer

Length: 1521 bp
ATGCTGGCCATGCTATATTGCATGGCAATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGGTTTCAATTGCCGAGGCTGCTGATGCAATTGGCATTCCACGATCACTGATTGATATACGTCATGAGGGATCTCACCGTGAACTTCCCGCTCTTCAGGTTGTTCGTAGCGCTTCAATTAAGGCCCTCGATTGGTTGAAGTTTTATTATTGGGAACCTCAGAAGAATGCAATTCCATTTCAAGGCACTGGAAGTGCTAACATCAGAAGTGAAATAAAGTCAAAGTTCCTTGAATTGGCTTCCTGCTTGAAAGCTAAAATGACTCCCCACTTGCGTTCTTCTCAAGTCAAAGAAAAACGTGGTTATAAAAAAGAAGTTACCAAGATATTGAAAAGTCTTGTTGGGTTGTATTCGTCTTTCTCCTCAGAAGTTGTATCTGTACTGTTGGAATTGTTGTTGAACACTAAAAGCTCCTCAGATTCATTGGACCTTCCAGTAAACACCCAAAAGGGCCTAAGCCTACCTACGTCGTTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGAAACCAGAGTTACGTCTAGCTCTTCTGAATGCTGTTCTTGATATGATTGAGACTCAGGAAGGAATGATGGGAAGAGGTTTGAACACATCATCAGACTCTGGGGCAAAGATAAGCCAAGTTGAACATCTCTCTTCTCTGTTTACATGGCTCGTTAGAAGTTTCAAAGGATTAAAGCCTCCTGAAAAAGATTCTGCCGATGAGGTTAAAGTTTCTTCAGCAGAAAAAACTATCTCGAAAGCAGTTCTGATGGATGTCCTACGTAAATGTCTTCTTGTATCAGCTTCTGCTAATAGCCAGCTCATGAATTCTGCCCTCCATCTTGCAAAGCTTACAGGAGACGACTATCTAGTGCAAAAACTCAACAAACTCTCTTTCTTAGTTTCATCAAATTTGGATGCTACTGAAGAAAACACTTGTGTTGCAAGTTCAAATAGTATATTTGTCCAGCAAGAAAAATCTATAAGTCAAGCAGCAAAGAAGCTTGAGTTGATTAAACGTCACCAAACGCAGCGCGAAGCAGTGAAGGGAACAAGAGATGCTGATGTTGGAGGTTCTAAAAGATGGGTTATGACAAAATCATGGACCCAGTGTCCTATTGGAATGTTGCCTTGTGCTGTTGGGTATTCCGGCTGTCTGCCTGTTCTGGACTATAATTGTGCATCTGAAAAGGATCCTGAAGAACTTGGAAGAAATGAGAAGTGGGAACCGAACTCATATAGTGGCAAGAGACAAGCTAGTTCTGATATGCAGCTACTTGATAACCCATGTGTTAAGAAGATGAGGGAGACTGTGGTAGATGATTGTGTATCAGATAAGGAGGATGTTGCATCCTCAGAATATGTCAATGCCCATCTCATGATCGGTGGTGTCTGGAAGATAGTTGGGGAAGAAGAAATACTTGCCATCAAGTCTGCTGTGAGGATACTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

506

Amino Acids

56.04

Weight (kDa)

8.89

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 2 - 78 1e-24 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AccBSI CCGCTC 1 cut(s) 167
AccII CGCG 1 cut(s) 1101
AciI CCGC 1 cut(s) 165
AclWI GGATC 3 cut(s) 154, 1253, 1266
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 3 cut(s) 575, 892, 976
AcuI CTGAAG 4 cut(s) 155, 786, 1011, 1284
AfaI GTAC 1 cut(s) 456
AfeI AGCGCT 1 cut(s) 187
AfiI CCNNNNNNNGG 3 cut(s) 207, 257, 310
AhdI GACNNNNNGTC 1 cut(s) 1176
AhlI ACTAGT 1 cut(s) 1513
Alw26I GTCTC 4 cut(s) 632, 918, 1309, 1367
AlwI GGATC 3 cut(s) 154, 1253, 1266
AlwNI CAGNNNCTG 2 cut(s) 686, 872
Aor51HI AGCGCT 1 cut(s) 187
AoxI GGCC 3 cut(s) 6, 198, 526
ApeKI GCWGC 4 cut(s) 95, 1058, 1096, 1336
ApoI RAATTY 3 cut(s) 575, 892, 976
AspLEI GCGC 2 cut(s) 188, 1101
AspS9I GGNCC 4 cut(s) 199, 502, 526, 1170
AsuHPI GGTGA 2 cut(s) 143, 1079
AvaII GGWCC 2 cut(s) 502, 1170
BalI TGGCCA 1 cut(s) 8
BamHI GGATCC 1 cut(s) 1258
BarI GAAGNNNNNNTAC 2 cut(s) 1467, 1499
BbsI GAAGAC 2 cut(s) 65, 848
BbvI GCAGC 4 cut(s) 82, 1070, 1108, 1348
BccI CCATC 6 cut(s) 649, 829, 912, 1143, 1446, 1499
BciVI GTATCC 1 cut(s) 1503
BcoDI GTCTC 4 cut(s) 632, 918, 1309, 1367
BcuI ACTAGT 1 cut(s) 1513
BfaI CTAG 4 cut(s) 605, 935, 1323, 1514
BfoI RGCGCY 1 cut(s) 189
BfuI GTATCC 1 cut(s) 1503
BisI GCNGC 4 cut(s) 96, 1059, 1097, 1337
BlsI GCNGC 4 cut(s) 97, 1060, 1098, 1338
Bme18I GGWCC 2 cut(s) 502, 1170
BmeRI GACNNNNNGTC 1 cut(s) 1176
BmgT120I GGNCC 4 cut(s) 199, 502, 526, 1170
BmiI GGNNCC 4 cut(s) 231, 1172, 1260, 1293
BmrI ACTGGG 1 cut(s) 1168
BmsI GCATC 5 cut(s) 91, 973, 1114, 1256, 1424
BmuI ACTGGG 1 cut(s) 1168
BpiI GAAGAC 2 cut(s) 65, 848
Bpu10I CCTNAGC 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 342, 1091
BsaAI YACGTR 1 cut(s) 848
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 257, 310
Bse1I ACTGG 3 cut(s) 268, 509, 1174
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 4 cut(s) 844, 988, 1414, 1415
BseLI CCNNNNNNNGG 3 cut(s) 207, 257, 310
BseMII CTCAG 5 cut(s) 248, 453, 504, 656, 1434
BseNI ACTGG 3 cut(s) 268, 509, 1174
BseRI GAGGAG 2 cut(s) 427, 478
BseXI GCAGC 4 cut(s) 82, 1070, 1108, 1348
Bsh1236I CGCG 1 cut(s) 1101
BshFI GGCC 3 cut(s) 8, 200, 528
BsiSI CCGG 1 cut(s) 1215
BslI CCNNNNNNNGG 3 cut(s) 207, 257, 310
BsmAI GTCTC 4 cut(s) 632, 918, 1309, 1367
BsmBI CGTCTC 1 cut(s) 918
BsmI GAATGC 3 cut(s) 111, 247, 622
BsnI GGCC 3 cut(s) 8, 200, 528
Bsp143I GATC 4 cut(s) 119, 146, 1258, 1446
BspACI CCGC 1 cut(s) 165
BspANI GGCC 3 cut(s) 8, 200, 528
BspCNI CTCAG 5 cut(s) 247, 452, 503, 655, 1433
BspFNI CGCG 1 cut(s) 1101
BspHI TCATGA 4 cut(s) 30, 139, 888, 1443
BspLI GGNNCC 4 cut(s) 231, 1172, 1260, 1293
BspPI GGATC 3 cut(s) 154, 1253, 1266
BspQI GCTCTTC 2 cut(s) 174, 615
BsrBI CCGCTC 1 cut(s) 167
BsrI ACTGG 3 cut(s) 268, 509, 1174
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 4 cut(s) 119, 146, 1258, 1446
Bst4CI ACNGT 3 cut(s) 155, 459, 1378
Bst6I CTCTTC 4 cut(s) 174, 615, 655, 724
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 2 cut(s) 6, 884
BstDEI CTNAG 7 cut(s) 234, 439, 490, 530, 642, 964, 1420
BstF5I GGATG 4 cut(s) 844, 988, 1414, 1415
BstFNI CGCG 1 cut(s) 1101
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 188, 1101
BstKTI GATC 4 cut(s) 122, 149, 1261, 1449
BstMAI GTCTC 4 cut(s) 632, 918, 1309, 1367
BstMBI GATC 4 cut(s) 119, 146, 1258, 1446
BstMWI GCNNNNNNNGC 3 cut(s) 10, 95, 101
BstSNI TACGTA 1 cut(s) 848
BstUI CGCG 1 cut(s) 1101
BstV1I GCAGC 4 cut(s) 82, 1070, 1108, 1348
BstV2I GAAGAC 2 cut(s) 65, 848
BstX2I RGATCY 2 cut(s) 146, 1258
BstYI RGATCY 2 cut(s) 146, 1258
BsuI GTATCC 1 cut(s) 1503
BsuRI GGCC 3 cut(s) 8, 200, 528
BtsCI GGATG 4 cut(s) 844, 988, 1414, 1415
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 4 cut(s) 122, 261, 1113, 1181
Cac8I GCNNGC 2 cut(s) 6, 884
CaiI CAGNNNCTG 2 cut(s) 686, 872
CciI TCATGA 4 cut(s) 30, 139, 888, 1443
CfoI GCGC 2 cut(s) 188, 1101
Cfr13I GGNCC 4 cut(s) 199, 502, 526, 1170
Csp6I GTAC 1 cut(s) 455
CviAII CATG 9 cut(s) 10, 22, 31, 140, 732, 889, 1167, 1353, 1444
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 7 cut(s) 234, 439, 490, 530, 642, 964, 1420
DpnI GATC 4 cut(s) 121, 148, 1260, 1448
DpnII GATC 4 cut(s) 119, 146, 1258, 1446
DriI GACNNNNNGTC 1 cut(s) 1176
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 4 cut(s) 174, 615, 655, 724
Eam1105I GACNNNNNGTC 1 cut(s) 1176
EarI CTCTTC 4 cut(s) 174, 615, 655, 724
Eco105I TACGTA 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 502, 1170
Eco47III AGCGCT 1 cut(s) 187
Eco57I CTGAAG 4 cut(s) 155, 786, 1011, 1284
EcoO109I RGGNCCY 2 cut(s) 199, 526
EcoRI GAATTC 1 cut(s) 892
Esp3I CGTCTC 1 cut(s) 918
FaeI CATG 9 cut(s) 13, 25, 34, 143, 735, 892, 1170, 1356, 1447
FalI AAGNNNNNCTT 2 cut(s) 1470, 1502
FatI CATG 9 cut(s) 9, 21, 30, 139, 731, 888, 1166, 1352, 1443
FauI CCCGC 1 cut(s) 172
Fnu4HI GCNGC 4 cut(s) 96, 1059, 1097, 1337
FokI GGATG 4 cut(s) 851, 995, 1402, 1421
Fsp4HI GCNGC 4 cut(s) 96, 1059, 1097, 1337
FspBI CTAG 4 cut(s) 605, 935, 1323, 1514
GlaI GCGC 2 cut(s) 187, 1100
GluI GCNGC 4 cut(s) 96, 1059, 1097, 1337
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 3 cut(s) 8, 200, 528
HapII CCGG 1 cut(s) 1215
HhaI GCGC 2 cut(s) 188, 1101
Hin1II CATG 9 cut(s) 13, 25, 34, 143, 735, 892, 1170, 1356, 1447
Hin6I GCGC 2 cut(s) 186, 1099
HinP1I GCGC 2 cut(s) 186, 1099
HindIII AAGCTT 3 cut(s) 560, 914, 1067
HinfI GANTC 5 cut(s) 337, 494, 640, 683, 776
HpaII CCGG 1 cut(s) 1215
HphI GGTGA 2 cut(s) 143, 1079
Hpy166II GTNNAC 3 cut(s) 158, 514, 729
Hpy8I GTNNAC 3 cut(s) 158, 514, 729
Hpy99I CGWCG 1 cut(s) 547
HpyAV CCTTC 3 cut(s) 515, 641, 1105
HpyCH4III ACNGT 3 cut(s) 155, 459, 1378
HpyCH4IV ACGT 6 cut(s) 136, 373, 542, 601, 847, 1084
HpyCH4V TGCA 9 cut(s) 21, 104, 245, 911, 940, 1010, 1247, 1336, 1415
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 95, 101
HpyF3I CTNAG 7 cut(s) 234, 439, 490, 530, 642, 964, 1420
HpySE526I ACGT 6 cut(s) 136, 373, 542, 601, 847, 1084
Hsp92II CATG 9 cut(s) 13, 25, 34, 143, 735, 892, 1170, 1356, 1447
HspAI GCGC 2 cut(s) 186, 1099
Kzo9I GATC 4 cut(s) 119, 146, 1258, 1446
LguI GCTCTTC 2 cut(s) 174, 615
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 4 cut(s) 82, 1070, 1108, 1348
LweI GCATC 5 cut(s) 91, 973, 1114, 1256, 1424
MaeI CTAG 4 cut(s) 605, 935, 1323, 1514
MaeII ACGT 6 cut(s) 136, 373, 542, 601, 847, 1084
MaeIII GTNAC 3 cut(s) 391, 597, 1085
MalI GATC 4 cut(s) 121, 148, 1260, 1448
MbiI CCGCTC 1 cut(s) 167
MboI GATC 4 cut(s) 119, 146, 1258, 1446
MfeI CAATTG 2 cut(s) 84, 105
MflI RGATCY 2 cut(s) 146, 1258
MlsI TGGCCA 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 3 cut(s) 331, 634, 677
MmeI TCCRAC 2 cut(s) 441, 1114
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 6 cut(s) 195, 758, 792, 1080, 1359, 1519
Msp20I TGGCCA 1 cut(s) 8
MspI CCGG 1 cut(s) 1215
MunI CAATTG 2 cut(s) 84, 105
Mva1269I GAATGC 3 cut(s) 111, 247, 622
MvnI CGCG 1 cut(s) 1101
MwoI GCNNNNNNNGC 3 cut(s) 10, 95, 101
NdeII GATC 4 cut(s) 119, 146, 1258, 1446
NlaIII CATG 9 cut(s) 13, 25, 34, 143, 735, 892, 1170, 1356, 1447
NlaIV GGNNCC 4 cut(s) 231, 1172, 1260, 1293
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 1085
PagI TCATGA 4 cut(s) 30, 139, 888, 1443
PciSI GCTCTTC 2 cut(s) 174, 615
PctI GAATGC 3 cut(s) 111, 247, 622
PfeI GAWTC 2 cut(s) 494, 776
PkrI GCNGC 4 cut(s) 97, 1060, 1098, 1338
PleI GAGTC 3 cut(s) 331, 634, 677
PpsI GAGTC 3 cut(s) 331, 634, 677
Ppu21I YACGTR 1 cut(s) 848
PsiI TTATAA 1 cut(s) 381
PspN4I GGNNCC 4 cut(s) 231, 1172, 1260, 1293
PspPI GGNCC 4 cut(s) 199, 502, 526, 1170
PstNI CAGNNNCTG 2 cut(s) 686, 872
PsuI RGATCY 2 cut(s) 146, 1258
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SapI GCTCTTC 2 cut(s) 174, 615
SaqAI TTAA 6 cut(s) 195, 758, 792, 1080, 1359, 1519
SatI GCNGC 4 cut(s) 96, 1059, 1097, 1337
Sau3AI GATC 4 cut(s) 119, 146, 1258, 1446
Sau96I GGNCC 4 cut(s) 199, 502, 526, 1170
SchI GAGTC 3 cut(s) 331, 634, 677
SfaNI GCATC 5 cut(s) 91, 973, 1114, 1256, 1424
SinI GGWCC 2 cut(s) 502, 1170
SmlI CTYRAG 2 cut(s) 357, 1070
SmoI CTYRAG 2 cut(s) 357, 1070
SnaBI TACGTA 1 cut(s) 848
SpeI ACTAGT 1 cut(s) 1513
SsiI CCGC 1 cut(s) 165
SspMI CTAG 4 cut(s) 605, 935, 1323, 1514
TaaI ACNGT 3 cut(s) 155, 459, 1378
TaiI ACGT 6 cut(s) 139, 376, 545, 604, 850, 1087
TaqI TCGA 2 cut(s) 204, 821
TatI WGTACW 1 cut(s) 454
TfiI GAWTC 2 cut(s) 494, 776
Tru1I TTAA 6 cut(s) 195, 758, 792, 1080, 1359, 1519
Tru9I TTAA 6 cut(s) 195, 758, 792, 1080, 1359, 1519
TscAI CASTG 4 cut(s) 129, 268, 1113, 1181
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 4 cut(s) 95, 1058, 1096, 1336
Tsp45I GTSAC 1 cut(s) 1085
TspDTI ATGAA 3 cut(s) 486, 905, 960
TspRI CASTG 4 cut(s) 129, 268, 1113, 1181
VpaK11BI GGWCC 2 cut(s) 502, 1170
XapI RAATTY 3 cut(s) 575, 892, 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 1181
XspI CTAG 4 cut(s) 605, 935, 1323, 1514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.