Rh7BG354200

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001924
Physical Location & Seq
Forward (+)
37105441 .. 37113756
8316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG354200.1

Sequence Viewer

Length: 1872 bp
ATGGAATCGCTTTTAGGGTTTATGGAAGAACCAGGGGATTATGACCAGGTAGAGACGACGTCGTCGTCGTCTTCGTCTTCGTACAGCCACAAATTGGTGCCGTGGTTGAGCTGGGACGAGTGGCTCTTCGTCTCTGAAGCTCTCTTTTCCAATTCACCTGATTCCGTCGCCTCCGCTTTGAGAAGAATATCAGCATGGAGAAGCAGAGGATGTGTTCCTGCTGTGATCGAAGTTACAGCTTCAATAATTGAAATTCAGCAAAAAGATCCTCACTTTAGAAAGGACCAGTCAAATGATGCTGTGGATAATTGCCGAGCAGACCAGTCCGGTGATTCTTCACTTTCTGAGGAAATGCTGGCCATGCTATATTGCATGGCAATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGGTTTCAATTGCTGAGGCTGCTGATGCAATTGGCATTCCACGATCACTGATTGATATTCGTCATGAGGGATCTCATCGTGAACTTCCCGCTCTTCAGGTTGTTCGTAGCGCTTCAATTAAGGCCCTTGATTGGTTGAAGTATTATTATTGGGAACCTCAGAAGAATGCAATTCCATTTCAAGGCACTGGAAGTGCTAACATCAGAAATGAAATAAAGTCAAAGTTCCGTGAATTGGCTTCCTGCTTGAGAGCTAAAATGACTCCCCACTTGCGTTCTTCTCAAGTCAAAGAAAAACGTGGTTATAAAAAAGATGTTACCAAGATATTGAAAAGTCTTGTTGGGTTGTATTCGTCTTTCTCCTCAGAAGTTGTATCTGTACTGTTGGAGTTGTTATTGAACACTAAAAGCTCTTCAGTTTCATCGGACCTTCCAGTAAACACCCAAAAGGGCCTAAGCCTACCTACGTCGTTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGAAACCAGAGTTACGTCTAGCTCTTCTGCATGCAGTTCTTGATATGATTGAGACTCAGGAAGGAATGATGGGAAGAGGGTTGAACACATCATCAGACTGTGGGGCAAAGATAAGCCAAGTTGAACATCTCTCTTCTCTGTTTACATGGCTCGTTAGAAGTTTCAAAGGATTAAAGCCTCCTGAAAAAGATTCTGCCGATGAGGTTAAAGTTTCTTCAGCGGAAAAAACTATCTCGAAAGCAGTTCTGATGGATGTCCTACGTAAATGTCTTCTTGTATCAGCTTCTGCTAATAGCCAGCTCATGAATTCTGCCCTCCATCTTGCAAAGCTTACAGGAGACGACTATCTAGTGCAAAAACTCAACAAACTCTCTTTCTTAGTTTCATCAAATTTGGATGCTACCGAAGAAAACACTTGTCTTGCAAGTTCAAATAGTATATTTGTTCAGCAAGAAAAATCTTTAAGTCAAGCAGCAAAGAAGCTTGAGTTGATTAAACGTCACCAAATGAAGCGCGAAGCAGTGAAGGGAACAAGAGATGCTGATGTTGGAGGTTCTAAAAGATGGGTTATGGCAAAATCATGGACCCAGTGTCCTATTGGAATGTTGCCTTGTGCTGTTGGGTATTCCAGCTGTCTGCCTGTTCTGGACTATAATTGTGCATCTGAAAAGGATCCTGAAGAACTTGGAAGAAACGAGAAGTGGGAACCGAACTCATATAGTGGCAAGAGACAAGCTAGTTCTGATATGCAGCTACTTGATAACCCATGTGTTAAGAAGATGAGGGAGACTGCGGTAGATGATTGTGTATCAGATAAGGAAGATGTTGCGTCCTCAGAATATGTCAATGGCCATCTCATGATCGGTGGTGTCTGGAAGAAAGTTGGGGAAGAAGAAATACTTGCCATCAAGTCTGCTGTGAGGATATTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

623

Amino Acids

69.16

Weight (kDa)

8.06

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 33 - 195 4.9e-44 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 732
AasI GACNNNNNNGTC 2 cut(s) 61, 64
AatII GACGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 94
AccBSI CCGCTC 1 cut(s) 518
AccII CGCG 1 cut(s) 1452
AciI CCGC 4 cut(s) 174, 516, 1157, 1730
AclWI GGATC 4 cut(s) 260, 505, 1604, 1617
AcoI YGGCCR 2 cut(s) 357, 1786
AcsI RAATTY 4 cut(s) 252, 926, 1243, 1327
AcuI CTGAAG 5 cut(s) 156, 506, 825, 1137, 1635
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 83, 807
AfeI AGCGCT 1 cut(s) 538
AfiI CCNNNNNNNGG 4 cut(s) 94, 558, 608, 661
AhdI GACNNNNNGTC 1 cut(s) 1527
AjnI CCWGG 2 cut(s) 31, 45
Alw26I GTCTC 6 cut(s) 47, 136, 983, 1269, 1660, 1718
AlwI GGATC 4 cut(s) 260, 505, 1604, 1617
AlwNI CAGNNNCTG 1 cut(s) 1223
Aor51HI AGCGCT 1 cut(s) 538
AoxI GGCC 4 cut(s) 357, 549, 877, 1786
ApeKI GCWGC 3 cut(s) 446, 1409, 1687
ApoI RAATTY 4 cut(s) 252, 926, 1243, 1327
AspLEI GCGC 2 cut(s) 539, 1452
AspS9I GGNCC 5 cut(s) 283, 550, 853, 877, 1521
AsuHPI GGTGA 3 cut(s) 147, 341, 1430
AvaII GGWCC 3 cut(s) 283, 853, 1521
BalI TGGCCA 2 cut(s) 359, 1788
BamHI GGATCC 1 cut(s) 1609
BanI GGYRCC 1 cut(s) 97
BarI GAAGNNNNNNTAC 2 cut(s) 1818, 1850
BbsI GAAGAC 4 cut(s) 63, 69, 416, 1199
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 3 cut(s) 433, 1421, 1699
BccI CCATC 6 cut(s) 1000, 1180, 1263, 1494, 1797, 1850
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 2 cut(s) 33, 47
BcoDI GTCTC 6 cut(s) 47, 136, 983, 1269, 1660, 1718
BfaI CTAG 3 cut(s) 956, 1286, 1674
BfoI RGCGCY 1 cut(s) 540
BisI GCNGC 3 cut(s) 447, 1410, 1688
BlsI GCNGC 3 cut(s) 448, 1411, 1689
Bme1390I CCNGG 2 cut(s) 33, 47
Bme18I GGWCC 3 cut(s) 283, 853, 1521
BmeRI GACNNNNNGTC 1 cut(s) 1527
BmgT120I GGNCC 5 cut(s) 283, 550, 853, 877, 1521
BmiI GGNNCC 5 cut(s) 99, 582, 1523, 1611, 1644
BmrFI CCNGG 2 cut(s) 33, 47
BmrI ACTGGG 1 cut(s) 1519
BmsI GCATC 5 cut(s) 286, 442, 1324, 1465, 1607
BmuI ACTGGG 1 cut(s) 1519
BpiI GAAGAC 4 cut(s) 63, 69, 416, 1199
Bpu10I CCTNAGC 2 cut(s) 441, 881
BpuEI CTTGAG 3 cut(s) 693, 694, 1442
BsaAI YACGTR 1 cut(s) 1199
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 32, 101
BsaWI WCCGGW 1 cut(s) 326
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 558, 608, 661
Bse1I ACTGG 5 cut(s) 286, 322, 619, 860, 1525
BseBI CCWGG 2 cut(s) 33, 47
BseDI CCNNGG 2 cut(s) 32, 101
BseGI GGATG 3 cut(s) 215, 1195, 1339
BseLI CCNNNNNNNGG 4 cut(s) 94, 558, 608, 661
BseMII CTCAG 6 cut(s) 336, 432, 599, 804, 1007, 1785
BseNI ACTGG 5 cut(s) 286, 322, 619, 860, 1525
BseRI GAGGAG 1 cut(s) 778
BseXI GCAGC 3 cut(s) 433, 1421, 1699
BseYI CCCAGC 1 cut(s) 111
Bsh1236I CGCG 1 cut(s) 1452
BshFI GGCC 4 cut(s) 359, 551, 879, 1788
BshNI GGYRCC 1 cut(s) 97
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 4 cut(s) 94, 558, 608, 661
BsmAI GTCTC 6 cut(s) 47, 136, 983, 1269, 1660, 1718
BsmBI CGTCTC 3 cut(s) 47, 136, 1269
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 2 cut(s) 462, 598
BsnI GGCC 4 cut(s) 359, 551, 879, 1788
Bsp143I GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
BspACI CCGC 4 cut(s) 174, 516, 1157, 1730
BspANI GGCC 4 cut(s) 359, 551, 879, 1788
BspCNI CTCAG 6 cut(s) 337, 433, 598, 803, 1006, 1784
BspFNI CGCG 1 cut(s) 1452
BspHI TCATGA 4 cut(s) 381, 490, 1239, 1794
BspLI GGNNCC 5 cut(s) 99, 582, 1523, 1611, 1644
BspPI GGATC 4 cut(s) 260, 505, 1604, 1617
BspQI GCTCTTC 4 cut(s) 131, 525, 844, 966
BspT107I GGYRCC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 518
BsrI ACTGG 5 cut(s) 286, 322, 619, 860, 1525
BssECI CCNNGG 2 cut(s) 32, 101
BssMI GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
BssNI GRCGYC 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 33, 47
Bst4CI ACNGT 2 cut(s) 810, 1037
Bst6I CTCTTC 6 cut(s) 131, 525, 844, 966, 1006, 1075
BstACI GRCGYC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 1199
BstC8I GCNNGC 3 cut(s) 357, 969, 1235
BstDEI CTNAG 8 cut(s) 345, 441, 585, 790, 881, 993, 1315, 1771
BstDSI CCRYGG 1 cut(s) 101
BstF5I GGATG 3 cut(s) 215, 1195, 1339
BstFNI CGCG 1 cut(s) 1452
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 2 cut(s) 539, 1452
BstKTI GATC 6 cut(s) 228, 268, 473, 500, 1612, 1800
BstMAI GTCTC 6 cut(s) 47, 136, 983, 1269, 1660, 1718
BstMBI GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
BstMWI GCNNNNNNNGC 3 cut(s) 361, 446, 452
BstNI CCWGG 2 cut(s) 33, 47
BstNSI RCATGY 1 cut(s) 971
BstSCI CCNGG 2 cut(s) 31, 45
BstSNI TACGTA 1 cut(s) 1199
BstUI CGCG 1 cut(s) 1452
BstV1I GCAGC 3 cut(s) 433, 1421, 1699
BstV2I GAAGAC 4 cut(s) 63, 69, 416, 1199
BstX2I RGATCY 3 cut(s) 265, 497, 1609
BstYI RGATCY 3 cut(s) 265, 497, 1609
BsuRI GGCC 4 cut(s) 359, 551, 879, 1788
BtgI CCRYGG 1 cut(s) 101
BtsCI GGATG 3 cut(s) 215, 1195, 1339
BtsI GCAGTG 1 cut(s) 1464
BtsIMutI CAGTG 4 cut(s) 473, 612, 1464, 1532
Cac8I GCNNGC 3 cut(s) 357, 969, 1235
CaiI CAGNNNCTG 1 cut(s) 1223
CciI TCATGA 4 cut(s) 381, 490, 1239, 1794
CfoI GCGC 2 cut(s) 539, 1452
Cfr13I GGNCC 5 cut(s) 283, 550, 853, 877, 1521
CseI GACGC 1 cut(s) 1755
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 2 cut(s) 82, 806
CviQI GTAC 2 cut(s) 82, 806
DdeI CTNAG 8 cut(s) 345, 441, 585, 790, 881, 993, 1315, 1771
DpnI GATC 6 cut(s) 227, 267, 472, 499, 1611, 1799
DpnII GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
DrdI GACNNNNNNGTC 2 cut(s) 61, 64
DriI GACNNNNNGTC 1 cut(s) 1527
DseDI GACNNNNNNGTC 2 cut(s) 61, 64
EaeI YGGCCR 2 cut(s) 357, 1786
Eam1104I CTCTTC 6 cut(s) 131, 525, 844, 966, 1006, 1075
Eam1105I GACNNNNNGTC 1 cut(s) 1527
EarI CTCTTC 6 cut(s) 131, 525, 844, 966, 1006, 1075
Eco105I TACGTA 1 cut(s) 1199
Eco47I GGWCC 3 cut(s) 283, 853, 1521
Eco47III AGCGCT 1 cut(s) 538
Eco57I CTGAAG 5 cut(s) 156, 506, 825, 1137, 1635
EcoO109I RGGNCCY 2 cut(s) 550, 877
EcoRI GAATTC 1 cut(s) 1243
EcoRII CCWGG 2 cut(s) 31, 45
Esp3I CGTCTC 3 cut(s) 47, 136, 1269
FalI AAGNNNNNCTT 2 cut(s) 1821, 1853
FaqI GGGAC 1 cut(s) 128
FauI CCCGC 1 cut(s) 523
Fnu4HI GCNGC 3 cut(s) 447, 1410, 1688
FokI GGATG 3 cut(s) 222, 1202, 1346
Fsp4HI GCNGC 3 cut(s) 447, 1410, 1688
FspBI CTAG 3 cut(s) 956, 1286, 1674
GlaI GCGC 2 cut(s) 538, 1451
GluI GCNGC 3 cut(s) 447, 1410, 1688
GsaI CCCAGC 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 540
HaeIII GGCC 4 cut(s) 359, 551, 879, 1788
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 1755
HhaI GCGC 2 cut(s) 539, 1452
Hin1I GRCGYC 1 cut(s) 59
Hin6I GCGC 2 cut(s) 537, 1450
HinP1I GCGC 2 cut(s) 537, 1450
HindIII AAGCTT 3 cut(s) 911, 1265, 1418
HinfI GANTC 6 cut(s) 5, 161, 332, 688, 991, 1127
HpaII CCGG 1 cut(s) 327
HphI GGTGA 3 cut(s) 147, 341, 1430
Hpy166II GTNNAC 3 cut(s) 509, 865, 1080
Hpy8I GTNNAC 3 cut(s) 509, 865, 1080
Hpy99I CGWCG 6 cut(s) 61, 64, 67, 70, 170, 898
HpyAV CCTTC 3 cut(s) 866, 992, 1456
HpyCH4III ACNGT 2 cut(s) 810, 1037
HpyCH4IV ACGT 6 cut(s) 59, 724, 893, 952, 1198, 1435
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 446, 452
HpyF3I CTNAG 8 cut(s) 345, 441, 585, 790, 881, 993, 1315, 1771
HpySE526I ACGT 6 cut(s) 59, 724, 893, 952, 1198, 1435
Hsp92I GRCGYC 1 cut(s) 59
HspAI GCGC 2 cut(s) 537, 1450
Kzo9I GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
LguI GCTCTTC 4 cut(s) 131, 525, 844, 966
Lsp1109I GCAGC 3 cut(s) 433, 1421, 1699
LweI GCATC 5 cut(s) 286, 442, 1324, 1465, 1607
MabI ACCWGGT 1 cut(s) 45
MaeI CTAG 3 cut(s) 956, 1286, 1674
MaeII ACGT 6 cut(s) 59, 724, 893, 952, 1198, 1435
MaeIII GTNAC 4 cut(s) 232, 742, 948, 1436
MalI GATC 6 cut(s) 227, 267, 472, 499, 1611, 1799
MbiI CCGCTC 1 cut(s) 518
MboI GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
MfeI CAATTG 2 cut(s) 435, 456
MflI RGATCY 3 cut(s) 265, 497, 1609
MlsI TGGCCA 2 cut(s) 359, 1788
MluNI TGGCCA 2 cut(s) 359, 1788
MlyI GAGTC 2 cut(s) 682, 985
MmeI TCCRAC 2 cut(s) 792, 1465
Mox20I TGGCCA 2 cut(s) 359, 1788
MscI TGGCCA 2 cut(s) 359, 1788
MseI TTAA 7 cut(s) 546, 1109, 1143, 1400, 1431, 1710, 1870
Msp20I TGGCCA 2 cut(s) 359, 1788
MspA1I CMGCKG 2 cut(s) 1157, 1569
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 33, 47
MunI CAATTG 2 cut(s) 435, 456
Mva1269I GAATGC 2 cut(s) 462, 598
MvaI CCWGG 2 cut(s) 33, 47
MvnI CGCG 1 cut(s) 1452
MwoI GCNNNNNNNGC 3 cut(s) 361, 446, 452
NdeII GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
NlaIV GGNNCC 5 cut(s) 99, 582, 1523, 1611, 1644
NmeAIII GCCGAG 1 cut(s) 338
NmuCI GTSAC 1 cut(s) 1436
NspI RCATGY 1 cut(s) 971
PaeI GCATGC 1 cut(s) 971
PagI TCATGA 4 cut(s) 381, 490, 1239, 1794
PciSI GCTCTTC 4 cut(s) 131, 525, 844, 966
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 65, 71
PctI GAATGC 2 cut(s) 462, 598
PfeI GAWTC 4 cut(s) 5, 161, 332, 1127
PflFI GACNNNGTC 2 cut(s) 58, 61
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 3 cut(s) 448, 1411, 1689
PleI GAGTC 2 cut(s) 682, 985
PpsI GAGTC 2 cut(s) 682, 985
Ppu21I YACGTR 1 cut(s) 1199
PsiI TTATAA 1 cut(s) 732
Psp6I CCWGG 2 cut(s) 31, 45
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 2 cut(s) 31, 45
PspN4I GGNNCC 5 cut(s) 99, 582, 1523, 1611, 1644
PspPI GGNCC 5 cut(s) 283, 550, 853, 877, 1521
PstNI CAGNNNCTG 1 cut(s) 1223
PsuI RGATCY 3 cut(s) 265, 497, 1609
PsyI GACNNNGTC 2 cut(s) 58, 61
PvuII CAGCTG 1 cut(s) 1569
RsaI GTAC 2 cut(s) 83, 807
RsaNI GTAC 2 cut(s) 82, 806
SapI GCTCTTC 4 cut(s) 131, 525, 844, 966
SaqAI TTAA 7 cut(s) 546, 1109, 1143, 1400, 1431, 1710, 1870
SatI GCNGC 3 cut(s) 447, 1410, 1688
Sau3AI GATC 6 cut(s) 225, 265, 470, 497, 1609, 1797
Sau96I GGNCC 5 cut(s) 283, 550, 853, 877, 1521
SchI GAGTC 2 cut(s) 682, 985
ScrFI CCNGG 2 cut(s) 33, 47
SexAI ACCWGGT 1 cut(s) 45
SfaNI GCATC 5 cut(s) 286, 442, 1324, 1465, 1607
SinI GGWCC 3 cut(s) 283, 853, 1521
SmlI CTYRAG 3 cut(s) 673, 708, 1421
SmoI CTYRAG 3 cut(s) 673, 708, 1421
SnaBI TACGTA 1 cut(s) 1199
SphI GCATGC 1 cut(s) 971
SsiI CCGC 4 cut(s) 174, 516, 1157, 1730
SspMI CTAG 3 cut(s) 956, 1286, 1674
StyD4I CCNGG 2 cut(s) 31, 45
TaaI ACNGT 2 cut(s) 810, 1037
TaiI ACGT 6 cut(s) 62, 727, 896, 955, 1201, 1438
TaqI TCGA 2 cut(s) 228, 1172
TatI WGTACW 1 cut(s) 805
TfiI GAWTC 4 cut(s) 5, 161, 332, 1127
Tru1I TTAA 7 cut(s) 546, 1109, 1143, 1400, 1431, 1710, 1870
Tru9I TTAA 7 cut(s) 546, 1109, 1143, 1400, 1431, 1710, 1870
TscAI CASTG 4 cut(s) 480, 619, 1464, 1532
TseFI GTSAC 1 cut(s) 1436
TseI GCWGC 3 cut(s) 446, 1409, 1687
Tsp45I GTSAC 1 cut(s) 1436
TspDTI ATGAA 5 cut(s) 651, 837, 1256, 1311, 1460
TspGWI ACGGA 2 cut(s) 154, 644
TspRI CASTG 4 cut(s) 480, 619, 1464, 1532
Tth111I GACNNNGTC 2 cut(s) 58, 61
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 3 cut(s) 283, 853, 1521
XapI RAATTY 4 cut(s) 252, 926, 1243, 1327
XceI RCATGY 1 cut(s) 971
XcmI CCANNNNNNNNNTGG 1 cut(s) 1532
XspI CTAG 3 cut(s) 956, 1286, 1674
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.