RLG00000001928

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001924
Physical Location & Seq
Reverse (-)
23138968 .. 23146494
7527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001928

Sequence Viewer

Length: 1863 bp
ATGGAATCGCTTTTAGGGTTTATGGAAGAACCAGGGGATTATGACCAGGTAGAGACGACGACGTCGTCTTCGTACAGCCACAAATTGGTGGCGTGGTTGAGCTGGGACGAGTGGCTCTTCGTCTCTGAAGCTCTCTTTTCCAATTCACCTCATTCCGTCTCCTCCGCTTTGAGAAGAATATCGGCATGGAGAAGCAGAGGATGTGTTCCTGCTGTGATTGAAGTTACAGCTTCAATAATCGAAATTCAGCAAAAAGATCCTTACTTTAGAAAGGACCAGTCAAATGTTGCTGTGGATAATTGCGGAGCAGATCAACCCACTGATTCTTCACTTTCTGAGGAAATGCTGGCCATGCTATATTGCATGGCAATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGGTTTCAATTGCAGAGGCTGCTGATGCAATTGGCATTCCACGATCACTGATTGATATACGTCATGAGGGATCTCACCGTGAACTTCCCGCTCTTCAGGTTGTTCGTAGCGCTTCAATTAAGGCCCTTGATTGGTTGAAGTTTTATTATTGGGAACCTCAGAAGAATGCAATTCCATTTCAAGGCACTGGAAGTGCTAACATCAGAAATGAAATAAAGTCAAAGTTCCTTGAATTGGCTTCCTGCTTGAGAGCTAAAATGACTCCCCACTTGCGTTCTTCTCAAGTCAAAGAAAAACGTGGTTATAAAAAAGATGTTACCAAGATAGTGAAAAGTCTTGTTGGGTTGTATTCGTCTTTCTCCTCAGAAGTTGTATCTGTACTGTTGGAATTGTTGTTGAACACTAAAAGCTCCTCAGATTCATTGGACCTCCCAGCAAACACCCAAAAGGGCCTAAGCCTACCTACGTCGTTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGAAACCAGAGTTAAGTCTAGCTCTTCTGAATGCTGTTCTTGATATGATTGAGACTCAGGAAGGAATGATGGGAAGAGGGTTGAACACATCATCAGACTCTGGGGCAAAGATAAGCCAAGTTGAACATCTCTCTTCTCTGTTTACATGGCTCGTTAGAAGTTTCAAAGGATTAAAGCCTCCTGAAAAAGATTCTGCCGACGAGGTTAAAGTTTCTTCAGCAGAAAAAACTATCTCGAAAGCAGTTCTGATGGATGTCCTACGTAAATGTCTTCTTGTATCAGCTTCTGCTAATAGCCAGCTCATGAATGCTGCCCTCCATCTTGCAAAGCTTACAGGAGATGACTATCTAGTGCAAAAACTCAACAAACTCTCTTTCTTAGTTTCATCAAATTTGGATGCTACTGAAGAAAACACTTGTGTTGCAAGTTCAAATAGTATATTTGTCCAGCAAGAAAAATCTATGAGTCAAGCAGCAAAGAAGCTTGAGTTGATTAAACGTCACCAAATGAAGCGCGAAGCAGTGAAGGAAACAAGAGATGCTGATGTTGGAGGTTCTAAAAGATGGGTTATGGCAAAATCATGGACCCAGTGTCCTATTGGAATGTTGCCTTGTGCTGTTGGGTACTCCGGCTGTCTGCCTGTTTTGGACTATAATTGTGCATCTGAAAAGGATCCTGAGGAACTTGGAAGAAACGAGAAGTGGGAACCGAACTCATATAGTGGCAAGAGACAAGCTAGTTCTGATATGCAGCTACTTGATAACCCATGTGTTAAGAAGATGAGGGAGACTCTGGTAGATGATTGTGTATCAGATAAGGAAGATGTTGCGTCCTCAGAATATGTCAATGGCCATCTCATGATCGGTGGTGTCTGGAAGAAAGTTGGGGAAGAAGAAATACTTGCCATCAAGTCTGCTGTGAGGATATTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

621

Amino Acids

68.79

Weight (kDa)

7.09

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 30 - 192 7e-43 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AasI GACNNNNNNGTC 2 cut(s) 61, 64
AatII GACGTC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 85
AccBSI CCGCTC 1 cut(s) 509
AccII CGCG 1 cut(s) 1443
AciI CCGC 3 cut(s) 165, 303, 507
AclWI GGATC 4 cut(s) 251, 496, 1595, 1608
AcoI YGGCCR 2 cut(s) 348, 1777
AcsI RAATTY 3 cut(s) 243, 917, 1318
AcuI CTGAAG 4 cut(s) 147, 497, 1128, 1353
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 3 cut(s) 74, 798, 1553
AfeI AGCGCT 1 cut(s) 529
AfiI CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
AhdI GACNNNNNGTC 1 cut(s) 1518
AjnI CCWGG 2 cut(s) 31, 45
Alw26I GTCTC 6 cut(s) 47, 127, 163, 974, 1651, 1709
AlwI GGATC 4 cut(s) 251, 496, 1595, 1608
AlwNI CAGNNNCTG 3 cut(s) 437, 1028, 1214
Aor51HI AGCGCT 1 cut(s) 529
AoxI GGCC 4 cut(s) 348, 540, 868, 1777
ApeKI GCWGC 4 cut(s) 437, 1238, 1400, 1678
ApoI RAATTY 3 cut(s) 243, 917, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AspLEI GCGC 2 cut(s) 530, 1443
AspS9I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
AsuHPI GGTGA 3 cut(s) 138, 485, 1421
AvaII GGWCC 3 cut(s) 274, 844, 1512
AxyI CCTNAGG 1 cut(s) 1605
BalI TGGCCA 2 cut(s) 350, 1779
BamHI GGATCC 1 cut(s) 1600
BarI GAAGNNNNNNTAC 2 cut(s) 1809, 1841
BbsI GAAGAC 3 cut(s) 60, 407, 1190
BbvI GCAGC 4 cut(s) 424, 1225, 1412, 1690
BccI CCATC 6 cut(s) 991, 1171, 1254, 1485, 1788, 1841
BciT130I CCWGG 2 cut(s) 33, 47
BcoDI GTCTC 6 cut(s) 47, 127, 163, 974, 1651, 1709
BfaI CTAG 3 cut(s) 947, 1277, 1665
BfoI RGCGCY 1 cut(s) 531
BisI GCNGC 4 cut(s) 438, 1239, 1401, 1679
BlsI GCNGC 4 cut(s) 439, 1240, 1402, 1680
Bme1390I CCNGG 2 cut(s) 33, 47
Bme18I GGWCC 3 cut(s) 274, 844, 1512
BmeRI GACNNNNNGTC 1 cut(s) 1518
BmgT120I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
BmiI GGNNCC 4 cut(s) 573, 1514, 1602, 1635
BmrFI CCNGG 2 cut(s) 33, 47
BmrI ACTGGG 1 cut(s) 1510
BmsI GCATC 4 cut(s) 433, 1315, 1456, 1598
BmuI ACTGGG 1 cut(s) 1510
BpiI GAAGAC 3 cut(s) 60, 407, 1190
BplI GAGNNNNNCTC 2 cut(s) 1702, 1734
Bpu10I CCTNAGC 1 cut(s) 872
BpuEI CTTGAG 3 cut(s) 684, 685, 1433
BsaAI YACGTR 1 cut(s) 1190
BsaBI GATNNNNATC 1 cut(s) 1272
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 32
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
Bse1I ACTGG 3 cut(s) 277, 610, 1516
Bse21I CCTNAGG 1 cut(s) 1605
Bse8I GATNNNNATC 1 cut(s) 1272
BseBI CCWGG 2 cut(s) 33, 47
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 3 cut(s) 206, 1186, 1330
BseJI GATNNNNATC 1 cut(s) 1272
BseLI CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
BseMII CTCAG 7 cut(s) 327, 590, 795, 846, 998, 1596, 1776
BseNI ACTGG 3 cut(s) 277, 610, 1516
BseRI GAGGAG 3 cut(s) 151, 769, 820
BseXI GCAGC 4 cut(s) 424, 1225, 1412, 1690
BseYI CCCAGC 2 cut(s) 102, 850
Bsh1236I CGCG 1 cut(s) 1443
BshFI GGCC 4 cut(s) 350, 542, 870, 1779
BsiSI CCGG 1 cut(s) 1557
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
BsmAI GTCTC 6 cut(s) 47, 127, 163, 974, 1651, 1709
BsmBI CGTCTC 3 cut(s) 47, 127, 163
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 4 cut(s) 453, 589, 964, 1240
BsnI GGCC 4 cut(s) 350, 542, 870, 1779
Bsp143I GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
BspACI CCGC 3 cut(s) 165, 303, 507
BspANI GGCC 4 cut(s) 350, 542, 870, 1779
BspCNI CTCAG 7 cut(s) 328, 589, 794, 845, 997, 1597, 1775
BspFNI CGCG 1 cut(s) 1443
BspHI TCATGA 4 cut(s) 372, 481, 1230, 1785
BspLI GGNNCC 4 cut(s) 573, 1514, 1602, 1635
BspPI GGATC 4 cut(s) 251, 496, 1595, 1608
BspQI GCTCTTC 3 cut(s) 122, 516, 957
BsrBI CCGCTC 1 cut(s) 509
BsrI ACTGG 3 cut(s) 277, 610, 1516
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
BssNI GRCGYC 1 cut(s) 62
Bst2UI CCWGG 2 cut(s) 33, 47
Bst4CI ACNGT 2 cut(s) 497, 801
Bst6I CTCTTC 5 cut(s) 122, 516, 957, 997, 1066
BstACI GRCGYC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 437
BstBAI YACGTR 1 cut(s) 1190
BstC8I GCNNGC 2 cut(s) 348, 1226
BstDEI CTNAG 9 cut(s) 336, 576, 781, 832, 872, 984, 1306, 1605, 1762
BstF5I GGATG 3 cut(s) 206, 1186, 1330
BstFNI CGCG 1 cut(s) 1443
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 2 cut(s) 530, 1443
BstKTI GATC 6 cut(s) 259, 313, 464, 491, 1603, 1791
BstMAI GTCTC 6 cut(s) 47, 127, 163, 974, 1651, 1709
BstMBI GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
BstMWI GCNNNNNNNGC 3 cut(s) 352, 437, 443
BstNI CCWGG 2 cut(s) 33, 47
BstSCI CCNGG 2 cut(s) 31, 45
BstSNI TACGTA 1 cut(s) 1190
BstUI CGCG 1 cut(s) 1443
BstV1I GCAGC 4 cut(s) 424, 1225, 1412, 1690
BstV2I GAAGAC 3 cut(s) 60, 407, 1190
BstX2I RGATCY 3 cut(s) 256, 488, 1600
BstYI RGATCY 3 cut(s) 256, 488, 1600
Bsu36I CCTNAGG 1 cut(s) 1605
BsuRI GGCC 4 cut(s) 350, 542, 870, 1779
BtsCI GGATG 3 cut(s) 206, 1186, 1330
BtsI GCAGTG 1 cut(s) 1455
BtsIMutI CAGTG 5 cut(s) 318, 464, 603, 1455, 1523
Cac8I GCNNGC 2 cut(s) 348, 1226
CaiI CAGNNNCTG 3 cut(s) 437, 1028, 1214
CciI TCATGA 4 cut(s) 372, 481, 1230, 1785
CfoI GCGC 2 cut(s) 530, 1443
Cfr13I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
CseI GACGC 1 cut(s) 1746
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 3 cut(s) 73, 797, 1552
CviQI GTAC 3 cut(s) 73, 797, 1552
DdeI CTNAG 9 cut(s) 336, 576, 781, 832, 872, 984, 1306, 1605, 1762
DpnI GATC 6 cut(s) 258, 312, 463, 490, 1602, 1790
DpnII GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
DrdI GACNNNNNNGTC 2 cut(s) 61, 64
DriI GACNNNNNGTC 1 cut(s) 1518
DseDI GACNNNNNNGTC 2 cut(s) 61, 64
EaeI YGGCCR 2 cut(s) 348, 1777
Eam1104I CTCTTC 5 cut(s) 122, 516, 957, 997, 1066
Eam1105I GACNNNNNGTC 1 cut(s) 1518
EarI CTCTTC 5 cut(s) 122, 516, 957, 997, 1066
Eco105I TACGTA 1 cut(s) 1190
Eco47I GGWCC 3 cut(s) 274, 844, 1512
Eco47III AGCGCT 1 cut(s) 529
Eco57I CTGAAG 4 cut(s) 147, 497, 1128, 1353
Eco81I CCTNAGG 1 cut(s) 1605
EcoO109I RGGNCCY 2 cut(s) 541, 868
EcoRII CCWGG 2 cut(s) 31, 45
Esp3I CGTCTC 3 cut(s) 47, 127, 163
FalI AAGNNNNNCTT 2 cut(s) 1812, 1844
FaqI GGGAC 1 cut(s) 119
FauI CCCGC 1 cut(s) 514
Fnu4HI GCNGC 4 cut(s) 438, 1239, 1401, 1679
FokI GGATG 3 cut(s) 213, 1193, 1337
Fsp4HI GCNGC 4 cut(s) 438, 1239, 1401, 1679
FspBI CTAG 3 cut(s) 947, 1277, 1665
GlaI GCGC 2 cut(s) 529, 1442
GluI GCNGC 4 cut(s) 438, 1239, 1401, 1679
GsaI CCCAGC 2 cut(s) 106, 854
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 4 cut(s) 350, 542, 870, 1779
HapII CCGG 1 cut(s) 1557
HgaI GACGC 1 cut(s) 1746
HhaI GCGC 2 cut(s) 530, 1443
Hin1I GRCGYC 1 cut(s) 62
Hin6I GCGC 2 cut(s) 528, 1441
HinP1I GCGC 2 cut(s) 528, 1441
HindIII AAGCTT 3 cut(s) 902, 1256, 1409
HinfI GANTC 9 cut(s) 5, 323, 679, 836, 982, 1025, 1118, 1393, 1717
HpaII CCGG 1 cut(s) 1557
HphI GGTGA 3 cut(s) 138, 485, 1421
Hpy166II GTNNAC 2 cut(s) 500, 1071
Hpy8I GTNNAC 2 cut(s) 500, 1071
Hpy99I CGWCG 5 cut(s) 61, 64, 67, 889, 1130
HpyAV CCTTC 2 cut(s) 983, 1447
HpyCH4III ACNGT 2 cut(s) 497, 801
HpyCH4IV ACGT 6 cut(s) 62, 478, 715, 884, 1189, 1426
HpyCH4V TGCA 9 cut(s) 363, 431, 446, 587, 1253, 1282, 1352, 1589, 1678
HpyF10VI GCNNNNNNNGC 3 cut(s) 352, 437, 443
HpyF3I CTNAG 9 cut(s) 336, 576, 781, 832, 872, 984, 1306, 1605, 1762
HpySE526I ACGT 6 cut(s) 62, 478, 715, 884, 1189, 1426
Hsp92I GRCGYC 1 cut(s) 62
HspAI GCGC 2 cut(s) 528, 1441
Kzo9I GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
LguI GCTCTTC 3 cut(s) 122, 516, 957
LmnI GCTCC 2 cut(s) 305, 833
Lsp1109I GCAGC 4 cut(s) 424, 1225, 1412, 1690
LweI GCATC 4 cut(s) 433, 1315, 1456, 1598
MabI ACCWGGT 1 cut(s) 45
MaeI CTAG 3 cut(s) 947, 1277, 1665
MaeII ACGT 6 cut(s) 62, 478, 715, 884, 1189, 1426
MaeIII GTNAC 3 cut(s) 223, 733, 1427
MalI GATC 6 cut(s) 258, 312, 463, 490, 1602, 1790
MbiI CCGCTC 1 cut(s) 509
MboI GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
MfeI CAATTG 2 cut(s) 426, 447
MflI RGATCY 3 cut(s) 256, 488, 1600
MlsI TGGCCA 2 cut(s) 350, 1779
MluNI TGGCCA 2 cut(s) 350, 1779
MlyI GAGTC 5 cut(s) 673, 976, 1019, 1402, 1711
MmeI TCCRAC 2 cut(s) 783, 1456
Mox20I TGGCCA 2 cut(s) 350, 1779
MscI TGGCCA 2 cut(s) 350, 1779
MseI TTAA 7 cut(s) 537, 941, 1100, 1134, 1422, 1701, 1861
Msp20I TGGCCA 2 cut(s) 350, 1779
MspI CCGG 1 cut(s) 1557
MspR9I CCNGG 2 cut(s) 33, 47
MunI CAATTG 2 cut(s) 426, 447
Mva1269I GAATGC 4 cut(s) 453, 589, 964, 1240
MvaI CCWGG 2 cut(s) 33, 47
MvnI CGCG 1 cut(s) 1443
MwoI GCNNNNNNNGC 3 cut(s) 352, 437, 443
NdeII GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
NlaIV GGNNCC 4 cut(s) 573, 1514, 1602, 1635
NmuCI GTSAC 1 cut(s) 1427
PagI TCATGA 4 cut(s) 372, 481, 1230, 1785
PciSI GCTCTTC 3 cut(s) 122, 516, 957
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 68
PctI GAATGC 4 cut(s) 453, 589, 964, 1240
PfeI GAWTC 4 cut(s) 5, 323, 836, 1118
PflFI GACNNNGTC 2 cut(s) 61, 64
PflMI CCANNNNNTGG 1 cut(s) 85
PkrI GCNGC 4 cut(s) 439, 1240, 1402, 1680
PleI GAGTC 5 cut(s) 673, 976, 1019, 1401, 1711
PpsI GAGTC 5 cut(s) 673, 976, 1019, 1401, 1711
Ppu21I YACGTR 1 cut(s) 1190
PsiI TTATAA 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 31, 45
PspFI CCCAGC 2 cut(s) 102, 850
PspGI CCWGG 2 cut(s) 31, 45
PspN4I GGNNCC 4 cut(s) 573, 1514, 1602, 1635
PspPI GGNCC 5 cut(s) 274, 541, 844, 868, 1512
PstNI CAGNNNCTG 3 cut(s) 437, 1028, 1214
PsuI RGATCY 3 cut(s) 256, 488, 1600
PsyI GACNNNGTC 2 cut(s) 61, 64
RsaI GTAC 3 cut(s) 74, 798, 1553
RsaNI GTAC 3 cut(s) 73, 797, 1552
SapI GCTCTTC 3 cut(s) 122, 516, 957
SaqAI TTAA 7 cut(s) 537, 941, 1100, 1134, 1422, 1701, 1861
SatI GCNGC 4 cut(s) 438, 1239, 1401, 1679
Sau3AI GATC 6 cut(s) 256, 310, 461, 488, 1600, 1788
Sau96I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
SchI GAGTC 5 cut(s) 673, 976, 1019, 1402, 1711
ScrFI CCNGG 2 cut(s) 33, 47
SexAI ACCWGGT 1 cut(s) 45
SfaNI GCATC 4 cut(s) 433, 1315, 1456, 1598
SinI GGWCC 3 cut(s) 274, 844, 1512
SmlI CTYRAG 3 cut(s) 664, 699, 1412
SmoI CTYRAG 3 cut(s) 664, 699, 1412
SnaBI TACGTA 1 cut(s) 1190
SsiI CCGC 3 cut(s) 165, 303, 507
SspMI CTAG 3 cut(s) 947, 1277, 1665
StyD4I CCNGG 2 cut(s) 31, 45
TaaI ACNGT 2 cut(s) 497, 801
TaiI ACGT 6 cut(s) 65, 481, 718, 887, 1192, 1429
TaqI TCGA 2 cut(s) 240, 1163
TatI WGTACW 1 cut(s) 796
TfiI GAWTC 4 cut(s) 5, 323, 836, 1118
Tru1I TTAA 7 cut(s) 537, 941, 1100, 1134, 1422, 1701, 1861
Tru9I TTAA 7 cut(s) 537, 941, 1100, 1134, 1422, 1701, 1861
TscAI CASTG 5 cut(s) 325, 471, 610, 1455, 1523
TseFI GTSAC 1 cut(s) 1427
TseI GCWGC 4 cut(s) 437, 1238, 1400, 1678
Tsp45I GTSAC 1 cut(s) 1427
TspDTI ATGAA 5 cut(s) 642, 828, 1247, 1302, 1451
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 5 cut(s) 325, 471, 610, 1455, 1523
Tth111I GACNNNGTC 2 cut(s) 61, 64
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 274, 844, 1512
XapI RAATTY 3 cut(s) 243, 917, 1318
XcmI CCANNNNNNNNNTGG 1 cut(s) 1523
XspI CTAG 3 cut(s) 947, 1277, 1665
ZraI GACGTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.