Prupe.3G132200_v2.0.a1

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001924
Physical Location & Seq
Reverse (-)
13687392 .. 13694469
7078 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G132200.9

Sequence Viewer

Length: 1872 bp
ATGGCGGCACTTTTAGGGTTTGTGGAAGAATCAACAGATTATGTCCAACTTGGGATATCAACGTCGTCGTCATCATCCTACAGCTACAAATTAGTGCCGTGGTTGAGCTGGGACGAGTGGCTCTTCGTCGACGAATCTCTCTTTTCGAATTCACCTAATTCTGTGGCCTCCGCTTTAAGAAGAATATCAGCATGGAGAAGCAGAGGATGTCTTCCTGTTGTAGTTGAACTTACGTCATCGATTATTGAAATTCAGCAAAAAGATCCTCACTTTAGAAAGGACCAGTCAAATAATGCTTTGGATAATTGCAGAGGGGATCAATCAAGTGATGCTTCACTTTCAGACGAAATGCTGGCCATGTTATATTGTATGGCAATAATGAGGCTTGTGAATGGTGTTGTTGAGAAGACACGGAAGAAAACAGAGGTTTCCATTGCTGTGGCTGCTGATGCAATTGGCATCCCACGCACACTGATTGATATTCGTCATGAGGGATCTCACCGTGAACTTCCTGCACTTGAGGTTGTACGTAGTGCTTCAGTTAAGGCCCTTGATTGGTTGAAGTACTATTACTGGGAGCCTCAGAAGAAAGCAATTCCATTTCATGGCAATGAAACTGCTGGCATCAAAACTGAGATAATGTCGAAATTCCATGAATTTGCCTTCTGCTTAAGAGTTAAGAACACTCCTCACTTGCGTTCTTCAAAAATCAAAGAAAAACGTGGTTCTAAGAAAGACCTTACCAAGATATTAAAAAGCCTTGTAGGGTTGTATTCTGCCTTCTCCTCGGAAGTTGTTTCTGTACTGTTGGAGTTCTTGTTGAAGGCTATAAATTCCTCAGATTCCTCAGAGCTTCCAGTAAACACCCAAAATAGCCCAAGCCTACAAATTTCATTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGGAACCAGAGTTACTTCTGGCTCTTCTGAATGCAGTTCTTGATATGATTGAGAATCAGGAAGCAGTGATATATGAGACGGGTTGGGTTCGCACATCATCAGACCATAGGGCAGAAACACTCCAAGTTGAACACCTCTCTTCTCTGTTTAAGTGGCTGGTCAAAAAATTTGAAGGATTAAAGCCTCATTTTGAAAAAGATTCTGTAGCTGAGATCAAAGTTTCTTCAGGAGAAAAAACAATATCGAAAGCAATTCTGATGGAACTCCTACGTAAATGTCTTGTTCTATCAGCCTCTGCTAATAACCAGCTCATGGATTCTGCCATCGATCTTGCTCAACTGACAGGGGACAACTACCTATTGGGAAAACTGAACAAACTATCTTCATTAGTTTCATCAAATTCGGATGTTACTGAAGAAGGTGATTCTCTTAAACATTCAAATAATGTATTTAAGAAGAAAGACGAATCTATGAGTGAAGCAGCAAAGAAGCTAGAGATGATTAAATGTCACAAAATAAAGAAAAAAGTAGTGAAGAGAACAGATGCTGATGCCAGAAATACTAACAGATGGGTTCTGGCAAAATCATGGAACCCGTGTCCAATTGGAATGTTGCCTCGAGCTGTTGGGTTTTCTGGTTGCCTTCCTGTTCTGGACTTTAATATTGTCCCAGAAAAGGTTTCTCAATTAATAGGAAGAAAAGAGAACTGGGAATTGAACCAGTGTAGTGGCAAGAGAGAAGCTAGTTCTGATTTGCACCTACTAGACAACCAATGTGTTAAGAAGTTGAGGGAAACAGAGGATGGCTGTGTATCAGACAGTGAGCATGTTTCATCCATAGAAGATGTCAGTGGCCATCTCATGATAGATGGGGTCTGGAAGAGAGTTGGGGAAGAAGAACTACTTGCCATCAAGTCTGCTTTAAGGATATTAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

624

Amino Acids

69.76

Weight (kDa)

7.56

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 605, 1246
AccI GTMKAC 1 cut(s) 129
AciI CCGC 2 cut(s) 5, 171
AclWI GGATC 3 cut(s) 257, 324, 502
AcoI YGGCCR 2 cut(s) 354, 1786
AcsI RAATTY 9 cut(s) 148, 249, 647, 656, 832, 888, 923, 1100, 1333
AcuI CTGAAG 3 cut(s) 522, 1143, 1368
AfaI GTAC 3 cut(s) 528, 566, 804
AfiI CCNNNNNNNGG 4 cut(s) 555, 605, 1246, 1609
AflII CTTAAG 1 cut(s) 670
AluBI AGCT 9 cut(s) 84, 108, 853, 910, 1142, 1243, 1426, 1556, 1676
AluI AGCT 9 cut(s) 84, 108, 853, 910, 1142, 1243, 1426, 1556, 1676
Alw26I GTCTC 1 cut(s) 1004
AlwI GGATC 3 cut(s) 257, 324, 502
AlwNI CAGNNNCTG 2 cut(s) 1229, 1481
Ama87I CYCGRG 1 cut(s) 1551
AoxI GGCC 4 cut(s) 165, 354, 546, 1786
ApeKI GCWGC 2 cut(s) 443, 1415
ApoI RAATTY 9 cut(s) 148, 249, 647, 656, 832, 888, 923, 1100, 1333
AseI ATTAAT 2 cut(s) 1622, 1865
AspS9I GGNCC 2 cut(s) 280, 547
AsuHPI GGTGA 3 cut(s) 144, 491, 1367
AsuII TTCGAA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 1551
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 2 cut(s) 356, 1788
BarI GAAGNNNNNNTAC 6 cut(s) 554, 586, 764, 796, 1818, 1850
BbsI GAAGAC 2 cut(s) 203, 413
BbvI GCAGC 2 cut(s) 430, 1427
BccI CCATC 7 cut(s) 1186, 1265, 1497, 1730, 1796, 1797, 1850
BceAI ACGGC 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 1004
BfaI CTAG 3 cut(s) 1427, 1677, 1697
BfmI CTRYAG 2 cut(s) 79, 1137
BfrI CTTAAG 1 cut(s) 670
BisI GCNGC 3 cut(s) 6, 444, 1416
BlsI GCNGC 3 cut(s) 7, 445, 1417
BmcAI AGTACT 1 cut(s) 566
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 1551
BmgT120I GGNCC 2 cut(s) 280, 547
BmiI GGNNCC 3 cut(s) 579, 939, 1526
BmrI ACTGGG 2 cut(s) 583, 1651
BmsI GCATC 6 cut(s) 319, 439, 468, 633, 1468, 1474
BmuI ACTGGG 2 cut(s) 583, 1651
BpiI GAAGAC 2 cut(s) 203, 413
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 539
Bsa29I ATCGAT 2 cut(s) 239, 1260
BsaAI YACGTR 2 cut(s) 530, 1205
BsaJI CCNNGG 2 cut(s) 98, 786
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 4 cut(s) 555, 605, 1246, 1609
Bse1I ACTGG 5 cut(s) 283, 578, 857, 1646, 1654
Bse3DI GCAATG 2 cut(s) 432, 616
BseCI ATCGAT 2 cut(s) 239, 1260
BseDI CCNNGG 2 cut(s) 98, 786
BseGI GGATG 6 cut(s) 74, 212, 459, 1345, 1741, 1766
BseLI CCNNNNNNNGG 4 cut(s) 555, 605, 1246, 1609
BseMI GCAATG 2 cut(s) 432, 616
BseMII CTCAG 5 cut(s) 596, 624, 852, 861, 1134
BseNI ACTGG 5 cut(s) 283, 578, 857, 1646, 1654
BseRI GAGGAG 2 cut(s) 678, 775
BseXI GCAGC 2 cut(s) 430, 1427
BseYI CCCAGC 1 cut(s) 108
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 4 cut(s) 167, 356, 548, 1788
BshVI ATCGAT 2 cut(s) 239, 1260
BsiHKCI CYCGRG 1 cut(s) 1551
BslFI GGGAC 3 cut(s) 125, 1295, 1586
BslI CCNNNNNNNGG 4 cut(s) 555, 605, 1246, 1609
BsmAI GTCTC 1 cut(s) 1004
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 3 cut(s) 125, 1295, 1586
BsmI GAATGC 1 cut(s) 970
BsnI GGCC 4 cut(s) 167, 356, 548, 1788
BsoBI CYCGRG 1 cut(s) 1551
Bsp119I TTCGAA 1 cut(s) 146
Bsp143I GATC 5 cut(s) 262, 316, 494, 1146, 1261
BspACI CCGC 2 cut(s) 5, 171
BspANI GGCC 4 cut(s) 167, 356, 548, 1788
BspCNI CTCAG 5 cut(s) 595, 625, 851, 860, 1135
BspDI ATCGAT 2 cut(s) 239, 1260
BspHI TCATGA 2 cut(s) 487, 1794
BspLI GGNNCC 3 cut(s) 579, 939, 1526
BspPI GGATC 3 cut(s) 257, 324, 502
BspQI GCTCTTC 2 cut(s) 128, 963
BspT104I TTCGAA 1 cut(s) 146
BspTI CTTAAG 1 cut(s) 670
BsrDI GCAATG 2 cut(s) 432, 616
BsrI ACTGG 5 cut(s) 283, 578, 857, 1646, 1654
BssECI CCNNGG 2 cut(s) 98, 786
BssMI GATC 5 cut(s) 262, 316, 494, 1146, 1261
Bst4CI ACNGT 3 cut(s) 503, 807, 1754
Bst6I CTCTTC 5 cut(s) 128, 963, 1078, 1463, 1808
BstAFI CTTAAG 1 cut(s) 670
BstBAI YACGTR 2 cut(s) 530, 1205
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 354, 622
BstDEI CTNAG 6 cut(s) 582, 633, 729, 838, 847, 1143
BstDSI CCRYGG 1 cut(s) 98
BstF5I GGATG 6 cut(s) 74, 212, 459, 1345, 1741, 1766
BstKTI GATC 5 cut(s) 265, 319, 497, 1149, 1264
BstMAI GTCTC 1 cut(s) 1004
BstMBI GATC 5 cut(s) 262, 316, 494, 1146, 1261
BstMWI GCNNNNNNNGC 3 cut(s) 443, 449, 465
BstNSI RCATGY 1 cut(s) 1763
BstSFI CTRYAG 2 cut(s) 79, 1137
BstSNI TACGTA 2 cut(s) 530, 1205
BstV1I GCAGC 2 cut(s) 430, 1427
BstV2I GAAGAC 2 cut(s) 203, 413
BstX2I RGATCY 2 cut(s) 262, 494
BstXI CCANNNNNNTGG 2 cut(s) 439, 1661
BstYI RGATCY 2 cut(s) 262, 494
Bsu15I ATCGAT 2 cut(s) 239, 1260
BsuRI GGCC 4 cut(s) 167, 356, 548, 1788
BsuTUI ATCGAT 2 cut(s) 239, 1260
BtgI CCRYGG 1 cut(s) 98
BtsCI GGATG 6 cut(s) 74, 212, 459, 1345, 1741, 1766
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 5 cut(s) 470, 1005, 1661, 1759, 1789
Cac8I GCNNGC 2 cut(s) 354, 622
CaiI CAGNNNCTG 2 cut(s) 1229, 1481
CciI TCATGA 2 cut(s) 487, 1794
Cfr13I GGNCC 2 cut(s) 280, 547
ClaI ATCGAT 2 cut(s) 239, 1260
Csp6I GTAC 3 cut(s) 527, 565, 803
CviAII CATG 9 cut(s) 192, 358, 488, 605, 653, 1246, 1521, 1760, 1795
CviQI GTAC 3 cut(s) 527, 565, 803
DdeI CTNAG 6 cut(s) 582, 633, 729, 838, 847, 1143
DpnI GATC 5 cut(s) 264, 318, 496, 1148, 1263
DpnII GATC 5 cut(s) 262, 316, 494, 1146, 1261
EaeI YGGCCR 2 cut(s) 354, 1786
Eam1104I CTCTTC 5 cut(s) 128, 963, 1078, 1463, 1808
EarI CTCTTC 5 cut(s) 128, 963, 1078, 1463, 1808
Eco105I TACGTA 2 cut(s) 530, 1205
Eco32I GATATC 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 3 cut(s) 522, 1143, 1368
Eco88I CYCGRG 1 cut(s) 1551
EcoO109I RGGNCCY 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 148
EcoRV GATATC 1 cut(s) 57
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 9 cut(s) 195, 361, 491, 608, 656, 1249, 1524, 1763, 1798
FalI AAGNNNNNCTT 4 cut(s) 316, 348, 1821, 1853
FaqI GGGAC 3 cut(s) 125, 1295, 1586
FatI CATG 9 cut(s) 191, 357, 487, 604, 652, 1245, 1520, 1759, 1794
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 6, 444, 1416
FokI GGATG 6 cut(s) 61, 219, 446, 1352, 1748, 1753
Fsp4HI GCNGC 3 cut(s) 6, 444, 1416
FspBI CTAG 3 cut(s) 1427, 1677, 1697
GluI GCNGC 3 cut(s) 6, 444, 1416
GsaI CCCAGC 1 cut(s) 112
HaeIII GGCC 4 cut(s) 167, 356, 548, 1788
Hin1II CATG 9 cut(s) 195, 361, 491, 608, 656, 1249, 1524, 1763, 1798
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 8 cut(s) 29, 134, 842, 988, 1133, 1250, 1358, 1400
HphI GGTGA 3 cut(s) 144, 491, 1367
Hpy166II GTNNAC 3 cut(s) 130, 506, 862
Hpy188III TCNNGA 7 cut(s) 488, 974, 992, 1161, 1586, 1795, 1810
Hpy8I GTNNAC 3 cut(s) 130, 506, 862
Hpy99I CGWCG 4 cut(s) 67, 70, 131, 134
HpyAV CCTTC 6 cut(s) 673, 790, 817, 1100, 1346, 1586
HpyCH4III ACNGT 3 cut(s) 503, 807, 1754
HpyCH4IV ACGT 5 cut(s) 62, 233, 529, 721, 1204
HpyCH4V TGCA 5 cut(s) 309, 452, 515, 968, 1690
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 449, 465
HpyF3I CTNAG 6 cut(s) 582, 633, 729, 838, 847, 1143
HpySE526I ACGT 5 cut(s) 62, 233, 529, 721, 1204
Hsp92II CATG 9 cut(s) 195, 361, 491, 608, 656, 1249, 1524, 1763, 1798
Kzo9I GATC 5 cut(s) 262, 316, 494, 1146, 1261
LguI GCTCTTC 2 cut(s) 128, 963
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 2 cut(s) 430, 1427
LweI GCATC 6 cut(s) 319, 439, 468, 633, 1468, 1474
MaeI CTAG 3 cut(s) 1427, 1677, 1697
MaeII ACGT 5 cut(s) 62, 233, 529, 721, 1204
MaeIII GTNAC 3 cut(s) 945, 1342, 1442
MalI GATC 5 cut(s) 264, 318, 496, 1148, 1263
MboI GATC 5 cut(s) 262, 316, 494, 1146, 1261
MfeI CAATTG 2 cut(s) 453, 1536
MflI RGATCY 2 cut(s) 262, 494
MlsI TGGCCA 2 cut(s) 356, 1788
MluNI TGGCCA 2 cut(s) 356, 1788
MmeI TCCRAC 2 cut(s) 70, 789
Mox20I TGGCCA 2 cut(s) 356, 1788
MscI TGGCCA 2 cut(s) 356, 1788
MslI CAYNNNNRTG 2 cut(s) 437, 609
Msp20I TGGCCA 2 cut(s) 356, 1788
MspCI CTTAAG 1 cut(s) 670
MunI CAATTG 2 cut(s) 453, 1536
Mva1269I GAATGC 1 cut(s) 970
MwoI GCNNNNNNNGC 3 cut(s) 443, 449, 465
NdeII GATC 5 cut(s) 262, 316, 494, 1146, 1261
NlaIII CATG 9 cut(s) 195, 361, 491, 608, 656, 1249, 1524, 1763, 1798
NlaIV GGNNCC 3 cut(s) 579, 939, 1526
NmuCI GTSAC 1 cut(s) 1442
NspI RCATGY 1 cut(s) 1763
NspV TTCGAA 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 1551
PagI TCATGA 2 cut(s) 487, 1794
PciSI GCTCTTC 2 cut(s) 128, 963
PctI GAATGC 1 cut(s) 970
PfeI GAWTC 8 cut(s) 29, 134, 842, 988, 1133, 1250, 1358, 1400
PflMI CCANNNNNTGG 2 cut(s) 605, 1246
PkrI GCNGC 3 cut(s) 7, 445, 1417
Ppu21I YACGTR 2 cut(s) 530, 1205
PshBI ATTAAT 2 cut(s) 1622, 1865
PspFI CCCAGC 1 cut(s) 108
PspN4I GGNNCC 3 cut(s) 579, 939, 1526
PspPI GGNCC 2 cut(s) 280, 547
PspXI VCTCGAGB 1 cut(s) 1551
PsrI GAACNNNNNNTAC 2 cut(s) 930, 962
PstNI CAGNNNCTG 2 cut(s) 1229, 1481
PsuI RGATCY 2 cut(s) 262, 494
RsaI GTAC 3 cut(s) 528, 566, 804
RsaNI GTAC 3 cut(s) 527, 565, 803
RseI CAYNNNNRTG 2 cut(s) 437, 609
SalI GTCGAC 1 cut(s) 128
SapI GCTCTTC 2 cut(s) 128, 963
SatI GCNGC 3 cut(s) 6, 444, 1416
Sau3AI GATC 5 cut(s) 262, 316, 494, 1146, 1261
Sau96I GGNCC 2 cut(s) 280, 547
ScaI AGTACT 1 cut(s) 566
SfaNI GCATC 6 cut(s) 319, 439, 468, 633, 1468, 1474
SfcI CTRYAG 2 cut(s) 79, 1137
Sfr274I CTCGAG 1 cut(s) 1551
SfuI TTCGAA 1 cut(s) 146
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 1 cut(s) 1551
SmiMI CAYNNNNRTG 2 cut(s) 437, 609
SmlI CTYRAG 3 cut(s) 518, 670, 1551
SmoI CTYRAG 3 cut(s) 518, 670, 1551
SnaBI TACGTA 2 cut(s) 530, 1205
SsiI CCGC 2 cut(s) 5, 171
SspI AATATT 1 cut(s) 1597
SspMI CTAG 3 cut(s) 1427, 1677, 1697
TaaI ACNGT 3 cut(s) 503, 807, 1754
TaiI ACGT 5 cut(s) 65, 236, 532, 724, 1207
TaqI TCGA 7 cut(s) 129, 146, 239, 644, 1178, 1260, 1552
TatI WGTACW 2 cut(s) 564, 802
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 8 cut(s) 29, 134, 842, 988, 1133, 1250, 1358, 1400
TscAI CASTG 5 cut(s) 477, 1005, 1661, 1759, 1789
TseFI GTSAC 1 cut(s) 1442
TseI GCWGC 2 cut(s) 443, 1415
Tsp45I GTSAC 1 cut(s) 1442
TspDTI ATGAA 7 cut(s) 593, 627, 669, 882, 1308, 1317, 1755
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 5 cut(s) 477, 1005, 1661, 1759, 1789
Van91I CCANNNNNTGG 2 cut(s) 605, 1246
Vha464I CTTAAG 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 2 cut(s) 1622, 1865
XapI RAATTY 9 cut(s) 148, 249, 647, 656, 832, 888, 923, 1100, 1333
XceI RCATGY 1 cut(s) 1763
XhoI CTCGAG 1 cut(s) 1551
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 3 cut(s) 1427, 1677, 1697
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.