RchiOBHm_Chr7g0223811

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001924
Physical Location & Seq
Forward (+)
45683618 .. 45693192
9575 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20040

Sequence Viewer

Length: 1863 bp
ATGGAAGCGCTTTTAGGGTTTATGGAAGAACCAGGGGATTATGATCAGGTAGAGACGACGTCGTCGTCGTCGTACAGCCACAAATTGGTGCCGTGGTTGAGCTGGGACGAGTGGCTCTTCGTCTCTGAATCTCTCTTTTCCAATTCACCTGATTCCGTCGCCTCCGCTTTGAGAAGAATATCGGCATGGAGAAGCAGAGGATGTGTTCCTGCTGTGATCGAAGTTACAGCTTCAATAATCGAAATTCAGCAAAAAGATCCTTACTTTAGAAAGGACCAGTCAAATGTTGCTGTGGGTAATTGCGGAGCAGATCAACCCACTGATTCTTCACTTTCTGAGGAAATGCTGGCCATGCTATATTGCATGGCAATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGGTTTCAATTGCCGAGGCTGCTGATGCAATTGGCATTCCACGATCACTGATTGATATACGTCATGAGGGATCTCACCGTGAACTTCCCGCTCTTCAGGTTGTTCGTAGTGCTTCAATTAAGGCCCTTGATTGGTTGAAGTTTTATTATTGGGAACCTCAGAAGAATGCAATTCCATTTCAAGGCACTGGAAGTGCTAACATCAGAAATGAAATAAAGTCAAAGTTCCTTGAATTGGCTTCCTGCTTGAAAGCTAAAATGACTCCCCACTTGCGTTCTTCTCAAGTCAAAGAAAAACGTGGTTATAAAAAAGAAGTTACCAAGATATTGAAAAGTCTTGTTGGGTTGTATTCGTCTTTCTCCTCAGAAGTTGTATCTGTACTGTTGGAATTGTTGTTGAACACTAAAAGCTCCTCAGATTCATTGGACCTTCCAGTAAACACCCAAAAGGACCTAAGCCTACCTACGTCGTTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGAAACCAGAGTTACGTCTAGCTCTTCTGAATGCTGTTCTTGATATGATTGAGACTCAGGAAGGAATGATGGGAAGAGGTTTGAACACATCATCAGACTCTGGGGCAAAGATAAGCCAAGTTGAACATCTCTCTTCTCTGTTTACATGGCTCATTAGAAGTTTCAAAGGATTAAAGCCTCCTGAAAAAGATTCTGCCGACGAGGTTAAAGTTTCTTCAGCAGAAAAAACTATCTCGAAAGCAGTTCTGATGGATGTCCTACGTAAATGTCTTCTTGTATCAGCTTCTGCTAATAGCCAGCTCATGAATTCTGCCCTCCATCTTGCAAAGCTTACAGGAGACGACTATCTAGTGCAAAAACTCAACAAACTCTCTTTCTTAGTTTCATCAAATTTGGATGCTACTGAAGAAAACACTTGTGTTGCAAGTTCAAATAGTATATTTGTCCAGCAAGAAAAATCTATAAGTCAAGCAGCAAAGAAGCTTGAGTTGATTAAACGTCACCAAATGCAGCGCGAAGCAGTGACGGGAACAAGAGATGCTGATGTTGGAGGTTCTAAAAGATGGGTTATGGCAAAATCATGGACCCAGTGTCCTATTGGAATGTTGCCTTGTGCTGTTGGGTATTCCGGCTGTCTGCCTGTTCTGGACTACAATTGTGCATCTGAAAAGGATCCTGAAGAACTTGGAAGAAATGAGAAGTGGGAACCGAACTCATCTAGTGGCAAGAGACAAGCTAGTTCTGATATGCAGCTACTTGATAACCCATGTGTTAAGAAGATGAGGGGGACTGTGGTAGATGATTGTGTATCAGATAAGGAGGATGTTGCATCCTCAGAATATGTCAATGCCCATCTCATGATCGGTGGTGTCTGGAAGAGAGTTGGGGAAGAAGAAATACTTGCCATCAAGTCTGCTGTGAGGATATTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

620

Amino Acids

68.65

Weight (kDa)

7.06

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 30 - 192 1.4e-43 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AasI GACNNNNNNGTC 2 cut(s) 61, 64
AatII GACGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 88
AccB7I CCANNNNNTGG 1 cut(s) 85
AccBSI CCGCTC 1 cut(s) 509
AccII CGCG 1 cut(s) 1443
AciI CCGC 3 cut(s) 165, 303, 507
AclWI GGATC 4 cut(s) 251, 496, 1595, 1608
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 4 cut(s) 243, 917, 1234, 1318
AcuI CTGAAG 4 cut(s) 497, 1128, 1353, 1626
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 74, 798
AfeI AGCGCT 1 cut(s) 9
AfiI CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
AhdI GACNNNNNGTC 1 cut(s) 1518
AjnI CCWGG 1 cut(s) 31
Alw26I GTCTC 5 cut(s) 47, 127, 974, 1260, 1651
AlwI GGATC 4 cut(s) 251, 496, 1595, 1608
AlwNI CAGNNNCTG 2 cut(s) 1028, 1214
Aor51HI AGCGCT 1 cut(s) 9
AoxI GGCC 2 cut(s) 348, 540
ApeKI GCWGC 4 cut(s) 437, 1400, 1438, 1678
ApoI RAATTY 4 cut(s) 243, 917, 1234, 1318
AspLEI GCGC 2 cut(s) 10, 1443
AspS9I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
AsuHPI GGTGA 3 cut(s) 138, 485, 1421
AvaII GGWCC 4 cut(s) 274, 844, 868, 1512
BalI TGGCCA 1 cut(s) 350
BamHI GGATCC 1 cut(s) 1600
BanI GGYRCC 1 cut(s) 88
BarI GAAGNNNNNNTAC 2 cut(s) 1809, 1841
BbsI GAAGAC 2 cut(s) 407, 1190
BbvI GCAGC 4 cut(s) 424, 1412, 1450, 1690
BccI CCATC 6 cut(s) 991, 1171, 1254, 1485, 1788, 1841
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 33
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 5 cut(s) 47, 127, 974, 1260, 1651
BfaI CTAG 4 cut(s) 947, 1277, 1647, 1665
BfoI RGCGCY 1 cut(s) 11
BisI GCNGC 4 cut(s) 438, 1401, 1439, 1679
BlsI GCNGC 4 cut(s) 439, 1402, 1440, 1680
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 4 cut(s) 274, 844, 868, 1512
BmeRI GACNNNNNGTC 1 cut(s) 1518
BmgT120I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
BmiI GGNNCC 5 cut(s) 90, 573, 1514, 1602, 1635
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 1 cut(s) 1510
BmsI GCATC 5 cut(s) 433, 1315, 1456, 1598, 1766
BmuI ACTGGG 1 cut(s) 1510
BpiI GAAGAC 2 cut(s) 407, 1190
Bpu10I CCTNAGC 1 cut(s) 872
BpuEI CTTGAG 2 cut(s) 684, 1433
BsaAI YACGTR 1 cut(s) 1190
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 32, 92, 432
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
Bse1I ACTGG 4 cut(s) 277, 610, 851, 1516
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 1 cut(s) 33
BseDI CCNNGG 3 cut(s) 32, 92, 432
BseGI GGATG 5 cut(s) 206, 1186, 1330, 1756, 1757
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
BseMII CTCAG 6 cut(s) 327, 590, 795, 846, 998, 1776
BseNI ACTGG 4 cut(s) 277, 610, 851, 1516
BseRI GAGGAG 2 cut(s) 769, 820
BseXI GCAGC 4 cut(s) 424, 1412, 1450, 1690
BseYI CCCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 1443
BshFI GGCC 2 cut(s) 350, 542
BshNI GGYRCC 1 cut(s) 88
BsiSI CCGG 1 cut(s) 1557
BslFI GGGAC 2 cut(s) 119, 1729
BslI CCNNNNNNNGG 4 cut(s) 85, 549, 599, 652
BsmAI GTCTC 5 cut(s) 47, 127, 974, 1260, 1651
BsmBI CGTCTC 3 cut(s) 47, 127, 1260
BsmFI GGGAC 2 cut(s) 119, 1729
BsmI GAATGC 3 cut(s) 453, 589, 964
BsnI GGCC 2 cut(s) 350, 542
Bsp143I GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
BspACI CCGC 3 cut(s) 165, 303, 507
BspANI GGCC 2 cut(s) 350, 542
BspCNI CTCAG 6 cut(s) 328, 589, 794, 845, 997, 1775
BspFNI CGCG 1 cut(s) 1443
BspHI TCATGA 4 cut(s) 372, 481, 1230, 1785
BspLI GGNNCC 5 cut(s) 90, 573, 1514, 1602, 1635
BspPI GGATC 4 cut(s) 251, 496, 1595, 1608
BspQI GCTCTTC 3 cut(s) 122, 516, 957
BspT107I GGYRCC 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 509
BsrI ACTGG 4 cut(s) 277, 610, 851, 1516
BssECI CCNNGG 3 cut(s) 32, 92, 432
BssMI GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
BssNI GRCGYC 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 497, 801, 1720
Bst6I CTCTTC 6 cut(s) 122, 516, 957, 997, 1066, 1799
BstACI GRCGYC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 1190
BstC8I GCNNGC 2 cut(s) 348, 1226
BstDEI CTNAG 8 cut(s) 336, 576, 781, 832, 872, 984, 1306, 1762
BstDSI CCRYGG 1 cut(s) 92
BstF5I GGATG 5 cut(s) 206, 1186, 1330, 1756, 1757
BstFNI CGCG 1 cut(s) 1443
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 2 cut(s) 10, 1443
BstKTI GATC 8 cut(s) 46, 219, 259, 313, 464, 491, 1603, 1791
BstMAI GTCTC 5 cut(s) 47, 127, 974, 1260, 1651
BstMBI GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
BstMWI GCNNNNNNNGC 3 cut(s) 352, 437, 443
BstNI CCWGG 1 cut(s) 33
BstSCI CCNGG 1 cut(s) 31
BstSNI TACGTA 1 cut(s) 1190
BstUI CGCG 1 cut(s) 1443
BstV1I GCAGC 4 cut(s) 424, 1412, 1450, 1690
BstV2I GAAGAC 2 cut(s) 407, 1190
BstX2I RGATCY 3 cut(s) 256, 488, 1600
BstYI RGATCY 3 cut(s) 256, 488, 1600
BsuRI GGCC 2 cut(s) 350, 542
BtgI CCRYGG 1 cut(s) 92
BtsCI GGATG 5 cut(s) 206, 1186, 1330, 1756, 1757
BtsI GCAGTG 1 cut(s) 1455
BtsIMutI CAGTG 5 cut(s) 318, 464, 603, 1455, 1523
Cac8I GCNNGC 2 cut(s) 348, 1226
CaiI CAGNNNCTG 2 cut(s) 1028, 1214
CciI TCATGA 4 cut(s) 372, 481, 1230, 1785
CfoI GCGC 2 cut(s) 10, 1443
Cfr13I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
Csp6I GTAC 2 cut(s) 73, 797
CviQI GTAC 2 cut(s) 73, 797
DdeI CTNAG 8 cut(s) 336, 576, 781, 832, 872, 984, 1306, 1762
DpnI GATC 8 cut(s) 45, 218, 258, 312, 463, 490, 1602, 1790
DpnII GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
DrdI GACNNNNNNGTC 2 cut(s) 61, 64
DriI GACNNNNNGTC 1 cut(s) 1518
DseDI GACNNNNNNGTC 2 cut(s) 61, 64
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 6 cut(s) 122, 516, 957, 997, 1066, 1799
Eam1105I GACNNNNNGTC 1 cut(s) 1518
EarI CTCTTC 6 cut(s) 122, 516, 957, 997, 1066, 1799
Eco105I TACGTA 1 cut(s) 1190
Eco47I GGWCC 4 cut(s) 274, 844, 868, 1512
Eco47III AGCGCT 1 cut(s) 9
Eco57I CTGAAG 4 cut(s) 497, 1128, 1353, 1626
EcoO109I RGGNCCY 2 cut(s) 541, 868
EcoRI GAATTC 1 cut(s) 1234
EcoRII CCWGG 1 cut(s) 31
Esp3I CGTCTC 3 cut(s) 47, 127, 1260
FalI AAGNNNNNCTT 2 cut(s) 1812, 1844
FaqI GGGAC 2 cut(s) 119, 1729
FauI CCCGC 1 cut(s) 514
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 438, 1401, 1439, 1679
FokI GGATG 5 cut(s) 213, 1193, 1337, 1744, 1763
Fsp4HI GCNGC 4 cut(s) 438, 1401, 1439, 1679
FspBI CTAG 4 cut(s) 947, 1277, 1647, 1665
GlaI GCGC 2 cut(s) 9, 1442
GluI GCNGC 4 cut(s) 438, 1401, 1439, 1679
GsaI CCCAGC 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 2 cut(s) 350, 542
HapII CCGG 1 cut(s) 1557
HhaI GCGC 2 cut(s) 10, 1443
Hin1I GRCGYC 1 cut(s) 59
Hin6I GCGC 2 cut(s) 8, 1441
HinP1I GCGC 2 cut(s) 8, 1441
HindIII AAGCTT 3 cut(s) 902, 1256, 1409
HinfI GANTC 8 cut(s) 128, 152, 323, 679, 836, 982, 1025, 1118
HpaII CCGG 1 cut(s) 1557
HphI GGTGA 3 cut(s) 138, 485, 1421
Hpy166II GTNNAC 3 cut(s) 500, 856, 1071
Hpy8I GTNNAC 3 cut(s) 500, 856, 1071
Hpy99I CGWCG 8 cut(s) 61, 64, 67, 70, 73, 161, 889, 1130
HpyAV CCTTC 2 cut(s) 857, 983
HpyCH4III ACNGT 3 cut(s) 497, 801, 1720
HpyCH4IV ACGT 7 cut(s) 59, 478, 715, 884, 943, 1189, 1426
HpyF10VI GCNNNNNNNGC 3 cut(s) 352, 437, 443
HpyF3I CTNAG 8 cut(s) 336, 576, 781, 832, 872, 984, 1306, 1762
HpySE526I ACGT 7 cut(s) 59, 478, 715, 884, 943, 1189, 1426
Hsp92I GRCGYC 1 cut(s) 59
HspAI GCGC 2 cut(s) 8, 1441
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
LguI GCTCTTC 3 cut(s) 122, 516, 957
LmnI GCTCC 2 cut(s) 305, 833
Lsp1109I GCAGC 4 cut(s) 424, 1412, 1450, 1690
LweI GCATC 5 cut(s) 433, 1315, 1456, 1598, 1766
MaeI CTAG 4 cut(s) 947, 1277, 1647, 1665
MaeII ACGT 7 cut(s) 59, 478, 715, 884, 943, 1189, 1426
MaeIII GTNAC 5 cut(s) 223, 733, 939, 1427, 1450
MalI GATC 8 cut(s) 45, 218, 258, 312, 463, 490, 1602, 1790
MbiI CCGCTC 1 cut(s) 509
MboI GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
MfeI CAATTG 3 cut(s) 426, 447, 1582
MflI RGATCY 3 cut(s) 256, 488, 1600
MlsI TGGCCA 1 cut(s) 350
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 3 cut(s) 673, 976, 1019
MmeI TCCRAC 2 cut(s) 783, 1456
Mox20I TGGCCA 1 cut(s) 350
MscI TGGCCA 1 cut(s) 350
MseI TTAA 6 cut(s) 537, 1100, 1134, 1422, 1701, 1861
Msp20I TGGCCA 1 cut(s) 350
MspI CCGG 1 cut(s) 1557
MspR9I CCNGG 1 cut(s) 33
MunI CAATTG 3 cut(s) 426, 447, 1582
Mva1269I GAATGC 3 cut(s) 453, 589, 964
MvaI CCWGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 1443
MwoI GCNNNNNNNGC 3 cut(s) 352, 437, 443
NdeII GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
NlaIV GGNNCC 5 cut(s) 90, 573, 1514, 1602, 1635
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 2 cut(s) 1427, 1450
PagI TCATGA 4 cut(s) 372, 481, 1230, 1785
PciSI GCTCTTC 3 cut(s) 122, 516, 957
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 65, 68
PctI GAATGC 3 cut(s) 453, 589, 964
PfeI GAWTC 5 cut(s) 128, 152, 323, 836, 1118
PflFI GACNNNGTC 2 cut(s) 58, 61
PflMI CCANNNNNTGG 1 cut(s) 85
PkrI GCNGC 4 cut(s) 439, 1402, 1440, 1680
PleI GAGTC 3 cut(s) 673, 976, 1019
PpsI GAGTC 3 cut(s) 673, 976, 1019
Ppu21I YACGTR 1 cut(s) 1190
PpuMI RGGWCCY 1 cut(s) 868
PsiI TTATAA 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 5 cut(s) 90, 573, 1514, 1602, 1635
PspPI GGNCC 5 cut(s) 274, 541, 844, 868, 1512
PspPPI RGGWCCY 1 cut(s) 868
PstNI CAGNNNCTG 2 cut(s) 1028, 1214
PsuI RGATCY 3 cut(s) 256, 488, 1600
PsyI GACNNNGTC 2 cut(s) 58, 61
RsaI GTAC 2 cut(s) 74, 798
RsaNI GTAC 2 cut(s) 73, 797
SapI GCTCTTC 3 cut(s) 122, 516, 957
SaqAI TTAA 6 cut(s) 537, 1100, 1134, 1422, 1701, 1861
SatI GCNGC 4 cut(s) 438, 1401, 1439, 1679
Sau3AI GATC 8 cut(s) 43, 216, 256, 310, 461, 488, 1600, 1788
Sau96I GGNCC 5 cut(s) 274, 541, 844, 868, 1512
SchI GAGTC 3 cut(s) 673, 976, 1019
ScrFI CCNGG 1 cut(s) 33
SfaNI GCATC 5 cut(s) 433, 1315, 1456, 1598, 1766
SinI GGWCC 4 cut(s) 274, 844, 868, 1512
SmlI CTYRAG 2 cut(s) 699, 1412
SmoI CTYRAG 2 cut(s) 699, 1412
SnaBI TACGTA 1 cut(s) 1190
SsiI CCGC 3 cut(s) 165, 303, 507
SspMI CTAG 4 cut(s) 947, 1277, 1647, 1665
StyD4I CCNGG 1 cut(s) 31
TaaI ACNGT 3 cut(s) 497, 801, 1720
TaiI ACGT 7 cut(s) 62, 481, 718, 887, 946, 1192, 1429
TaqI TCGA 3 cut(s) 219, 240, 1163
TatI WGTACW 1 cut(s) 796
TfiI GAWTC 5 cut(s) 128, 152, 323, 836, 1118
Tru1I TTAA 6 cut(s) 537, 1100, 1134, 1422, 1701, 1861
Tru9I TTAA 6 cut(s) 537, 1100, 1134, 1422, 1701, 1861
TscAI CASTG 5 cut(s) 325, 471, 610, 1455, 1523
TseFI GTSAC 2 cut(s) 1427, 1450
TseI GCWGC 4 cut(s) 437, 1400, 1438, 1678
Tsp45I GTSAC 2 cut(s) 1427, 1450
TspDTI ATGAA 4 cut(s) 642, 828, 1247, 1302
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 5 cut(s) 325, 471, 610, 1455, 1523
Tth111I GACNNNGTC 2 cut(s) 58, 61
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 4 cut(s) 274, 844, 868, 1512
XapI RAATTY 4 cut(s) 243, 917, 1234, 1318
XcmI CCANNNNNNNNNTGG 1 cut(s) 1523
XspI CTAG 4 cut(s) 947, 1277, 1647, 1665
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.