Rw7G030840

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001924
Physical Location & Seq
Forward (+)
43744485 .. 43753828
9344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030840.1

Sequence Viewer

Length: 1866 bp
ATGGAGGCGCTTTTAGGGTTTATGGAAGAACCAGGGGATTATGACCAGGTAGAGACGACGTCGTCTTCGTCGTCGTACAGCCACAAATTGGTGCCGTGGTTGAGCTGGGACGAGTGGCTCTTCGTCTCTGAATCTCTCTTTTCCAACTCACCTGATTCCGTCGCCTCCGCTTTGAGAAGAATATCGGCATGGAGAAGCAGAGGATGTGTTCCTGCTGTGATCGAAGTTACAGCTTCAATAATCGAAATTCAGCAAAAAGATCCTTACTTTAGAAAGGACCAGTCAAATGTTGCTGTGGGTAATTGCGGAGCAGATAAACCCACTCATTCTTCACTTTCTGAGGAAATGCTGGCCATGCTATATTGCATGGCAATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGGTTTCAATTGCAGAGGCTGCTGATGCAATTGGCATTCCACGATCACTGATTGATATACGTCATGAGGGATCTCACCGTGAACTTCCCGCTCTTCAGGTTGTTCGTAGCGCTTCAATTAAGGCCCTTGATTGGTTGAAGTTTTATTATTGGGAACCTCAGAAGAATGCAATTCCATTTCAAGGCACTGGAAGTGCTAACATCAGAAATGAAATAAAGTCAAAGTTCCTTGAATTGGCTTCCTGCTTGAAAGCTAAAATGACTCCCCACTTGCGTTCTTCTCAAGTTAAAGAAAAACGTGGTTATAAGAAAGATGTTACCAAGATATTGAAAAGTCTTGTTGGGTTGTATTCGTCTTTCTCCTCAGAAGTTGTATCTGTACTGTTGGAATTGTTGTTGAACACTAAAAGCTCCTCAGATTCATTGGACCTTCCAGTAAACACCCAAAAGGGCCTAAGCCTACCTACGTCGTTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAATAAGAAACCAGAGTTACGTCTAGCTCTTCTGAATGCTGTTCTTGATATGATTGAGACTCAGGAAGGAATGATGGGAAGAGGTTTGAACACATCATCAGACTCTGGGGCAAAGATAAGCCAAGTTGAACATCTCTCTTCTCTGTTTACATGGCTCGTTAGAAGTTTCAAAGGATTAAAGCCTCCAGAAAAAGATTCTGCCGACGAGGTTAAAGTTTCTTCAGCAGAAAAAACTATCTCGAAAGCAGTTCTGATGGATGTCCTACGTAAATGTCTTCTTGTATCAGCTTCTGCTAATAGCCAGCTCATGAATTCTGCCCTCCATCTTGCAAAGCTTACAGGAGACGACTATCTAGTGCAAAAACTCAACAAACTCTCTTTCTTAGTTTCATCAAATTTGGATGCTACTGAAGAAAACACTTGTGTTGCAAGTTCAAATAGTATATTTGTCCAGCAAGAAAAATCTATAAGTCAAGCAGCAAAGAAGCTTGAGTTGATTAAACGTCACCAAATGCAGCGCGAAGCAGTGAAGGGAACAAGAGATGCTGATGTTGGAGGTTCTAAAAGATGGGTTATGGCAAAATCATGGACCCAGTGTCCTATTGGAATGTTGCCTTGTGCTGTTGGGTATTCCAGCTGTCTGCCTGTTCTGGACTATAATTGTGCATCTGAAAAGGATCCTGAAGAACTTGGAAGAAATGAGAAGTGGGAACCGAACTCATATAGTGGCAAGAGACAAGCTAGTTCTGATATGCAGCTACTTGATAACCCATGTGTTAAGAAGATGAGGGAGACTGTGGTAGATGATTGTGTATCAGATAAGGATGATGTTGCATCCTCAGAATATGTCAATGGCCATCTCATGATCGGTGGTGTCTGGAAGAAAGTTGGGGAAGAAGAAATACTTGCCATCAAGTCTGCGGTGAGGATATTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

621

Amino Acids

68.83

Weight (kDa)

8.08

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 31 - 193 1.4e-43 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AasI GACNNNNNNGTC 1 cut(s) 61
AatII GACGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 1 cut(s) 88
AccBSI CCGCTC 1 cut(s) 512
AccII CGCG 1 cut(s) 1446
AciI CCGC 4 cut(s) 168, 306, 510, 1847
AclWI GGATC 4 cut(s) 254, 499, 1598, 1611
AcoI YGGCCR 2 cut(s) 351, 1780
AcsI RAATTY 4 cut(s) 246, 920, 1237, 1321
AcuI CTGAAG 4 cut(s) 500, 1131, 1356, 1629
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 77, 801
AfeI AGCGCT 1 cut(s) 532
AfiI CCNNNNNNNGG 4 cut(s) 88, 552, 602, 655
AhdI GACNNNNNGTC 1 cut(s) 1521
AjnI CCWGG 2 cut(s) 31, 45
Alw26I GTCTC 6 cut(s) 47, 130, 977, 1263, 1654, 1712
AlwI GGATC 4 cut(s) 254, 499, 1598, 1611
AlwNI CAGNNNCTG 3 cut(s) 440, 1031, 1217
Aor51HI AGCGCT 1 cut(s) 532
AoxI GGCC 4 cut(s) 351, 543, 871, 1780
ApeKI GCWGC 4 cut(s) 440, 1403, 1441, 1681
ApoI RAATTY 4 cut(s) 246, 920, 1237, 1321
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 3 cut(s) 10, 533, 1446
AspS9I GGNCC 5 cut(s) 277, 544, 847, 871, 1515
AsuHPI GGTGA 4 cut(s) 141, 488, 1424, 1861
AvaII GGWCC 3 cut(s) 277, 847, 1515
BalI TGGCCA 2 cut(s) 353, 1782
BamHI GGATCC 1 cut(s) 1603
BanI GGYRCC 1 cut(s) 91
BarI GAAGNNNNNNTAC 2 cut(s) 1812, 1844
BbsI GAAGAC 3 cut(s) 57, 410, 1193
BbvI GCAGC 4 cut(s) 427, 1415, 1453, 1693
BccI CCATC 6 cut(s) 994, 1174, 1257, 1488, 1791, 1844
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 2 cut(s) 33, 47
BcoDI GTCTC 6 cut(s) 47, 130, 977, 1263, 1654, 1712
BfaI CTAG 3 cut(s) 950, 1280, 1668
BfoI RGCGCY 2 cut(s) 11, 534
BisI GCNGC 4 cut(s) 441, 1404, 1442, 1682
BlsI GCNGC 4 cut(s) 442, 1405, 1443, 1683
Bme1390I CCNGG 2 cut(s) 33, 47
Bme18I GGWCC 3 cut(s) 277, 847, 1515
BmeRI GACNNNNNGTC 1 cut(s) 1521
BmgT120I GGNCC 5 cut(s) 277, 544, 847, 871, 1515
BmiI GGNNCC 5 cut(s) 93, 576, 1517, 1605, 1638
BmrFI CCNGG 2 cut(s) 33, 47
BmrI ACTGGG 1 cut(s) 1513
BmsI GCATC 5 cut(s) 436, 1318, 1459, 1601, 1769
BmuI ACTGGG 1 cut(s) 1513
BpiI GAAGAC 3 cut(s) 57, 410, 1193
BpmI CTGGAG 1 cut(s) 1095
Bpu10I CCTNAGC 1 cut(s) 875
BpuEI CTTGAG 2 cut(s) 687, 1436
BsaAI YACGTR 1 cut(s) 1193
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 32, 95
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 552, 602, 655
Bse1I ACTGG 4 cut(s) 280, 613, 854, 1519
BseBI CCWGG 2 cut(s) 33, 47
BseDI CCNNGG 2 cut(s) 32, 95
BseGI GGATG 5 cut(s) 209, 1189, 1333, 1756, 1760
BseLI CCNNNNNNNGG 4 cut(s) 88, 552, 602, 655
BseMII CTCAG 6 cut(s) 330, 593, 798, 849, 1001, 1779
BseNI ACTGG 4 cut(s) 280, 613, 854, 1519
BseRI GAGGAG 2 cut(s) 772, 823
BseXI GCAGC 4 cut(s) 427, 1415, 1453, 1693
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 1446
BshFI GGCC 4 cut(s) 353, 545, 873, 1782
BshNI GGYRCC 1 cut(s) 91
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 4 cut(s) 88, 552, 602, 655
BsmAI GTCTC 6 cut(s) 47, 130, 977, 1263, 1654, 1712
BsmBI CGTCTC 3 cut(s) 47, 130, 1263
BsmFI GGGAC 1 cut(s) 122
BsmI GAATGC 3 cut(s) 456, 592, 967
BsnI GGCC 4 cut(s) 353, 545, 873, 1782
Bsp143I GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
BspACI CCGC 4 cut(s) 168, 306, 510, 1847
BspANI GGCC 4 cut(s) 353, 545, 873, 1782
BspCNI CTCAG 6 cut(s) 331, 592, 797, 848, 1000, 1778
BspFNI CGCG 1 cut(s) 1446
BspHI TCATGA 4 cut(s) 375, 484, 1233, 1788
BspLI GGNNCC 5 cut(s) 93, 576, 1517, 1605, 1638
BspPI GGATC 4 cut(s) 254, 499, 1598, 1611
BspQI GCTCTTC 3 cut(s) 125, 519, 960
BspT107I GGYRCC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 512
BsrI ACTGG 4 cut(s) 280, 613, 854, 1519
BssECI CCNNGG 2 cut(s) 32, 95
BssMI GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
BssNI GRCGYC 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 33, 47
Bst4CI ACNGT 3 cut(s) 500, 804, 1723
Bst6I CTCTTC 5 cut(s) 125, 519, 960, 1000, 1069
BstACI GRCGYC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 1193
BstC8I GCNNGC 2 cut(s) 351, 1229
BstDEI CTNAG 8 cut(s) 339, 579, 784, 835, 875, 987, 1309, 1765
BstDSI CCRYGG 1 cut(s) 95
BstF5I GGATG 5 cut(s) 209, 1189, 1333, 1756, 1760
BstFNI CGCG 1 cut(s) 1446
BstH2I RGCGCY 2 cut(s) 11, 534
BstHHI GCGC 3 cut(s) 10, 533, 1446
BstKTI GATC 6 cut(s) 222, 262, 467, 494, 1606, 1794
BstMAI GTCTC 6 cut(s) 47, 130, 977, 1263, 1654, 1712
BstMBI GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
BstMWI GCNNNNNNNGC 3 cut(s) 355, 440, 446
BstNI CCWGG 2 cut(s) 33, 47
BstSCI CCNGG 2 cut(s) 31, 45
BstSNI TACGTA 1 cut(s) 1193
BstUI CGCG 1 cut(s) 1446
BstV1I GCAGC 4 cut(s) 427, 1415, 1453, 1693
BstV2I GAAGAC 3 cut(s) 57, 410, 1193
BstX2I RGATCY 3 cut(s) 259, 491, 1603
BstYI RGATCY 3 cut(s) 259, 491, 1603
BsuRI GGCC 4 cut(s) 353, 545, 873, 1782
BtgI CCRYGG 1 cut(s) 95
BtsCI GGATG 5 cut(s) 209, 1189, 1333, 1756, 1760
BtsI GCAGTG 1 cut(s) 1458
BtsIMutI CAGTG 4 cut(s) 467, 606, 1458, 1526
Cac8I GCNNGC 2 cut(s) 351, 1229
CaiI CAGNNNCTG 3 cut(s) 440, 1031, 1217
CciI TCATGA 4 cut(s) 375, 484, 1233, 1788
CfoI GCGC 3 cut(s) 10, 533, 1446
Cfr13I GGNCC 5 cut(s) 277, 544, 847, 871, 1515
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 2 cut(s) 76, 800
CviQI GTAC 2 cut(s) 76, 800
DdeI CTNAG 8 cut(s) 339, 579, 784, 835, 875, 987, 1309, 1765
DpnI GATC 6 cut(s) 221, 261, 466, 493, 1605, 1793
DpnII GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
DrdI GACNNNNNNGTC 1 cut(s) 61
DriI GACNNNNNGTC 1 cut(s) 1521
DseDI GACNNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 2 cut(s) 351, 1780
Eam1104I CTCTTC 5 cut(s) 125, 519, 960, 1000, 1069
Eam1105I GACNNNNNGTC 1 cut(s) 1521
EarI CTCTTC 5 cut(s) 125, 519, 960, 1000, 1069
Eco105I TACGTA 1 cut(s) 1193
Eco47I GGWCC 3 cut(s) 277, 847, 1515
Eco47III AGCGCT 1 cut(s) 532
Eco57I CTGAAG 4 cut(s) 500, 1131, 1356, 1629
EcoO109I RGGNCCY 2 cut(s) 544, 871
EcoRI GAATTC 1 cut(s) 1237
EcoRII CCWGG 2 cut(s) 31, 45
Esp3I CGTCTC 3 cut(s) 47, 130, 1263
FalI AAGNNNNNCTT 2 cut(s) 1815, 1847
FaqI GGGAC 1 cut(s) 122
FauI CCCGC 1 cut(s) 517
Fnu4HI GCNGC 4 cut(s) 441, 1404, 1442, 1682
FokI GGATG 5 cut(s) 216, 1196, 1340, 1747, 1763
Fsp4HI GCNGC 4 cut(s) 441, 1404, 1442, 1682
FspBI CTAG 3 cut(s) 950, 1280, 1668
GlaI GCGC 3 cut(s) 9, 532, 1445
GluI GCNGC 4 cut(s) 441, 1404, 1442, 1682
GsaI CCCAGC 1 cut(s) 109
GsuI CTGGAG 1 cut(s) 1095
HaeII RGCGCY 2 cut(s) 11, 534
HaeIII GGCC 4 cut(s) 353, 545, 873, 1782
HhaI GCGC 3 cut(s) 10, 533, 1446
Hin1I GRCGYC 1 cut(s) 59
Hin6I GCGC 3 cut(s) 8, 531, 1444
HinP1I GCGC 3 cut(s) 8, 531, 1444
HindIII AAGCTT 3 cut(s) 905, 1259, 1412
HinfI GANTC 7 cut(s) 131, 155, 682, 839, 985, 1028, 1121
HphI GGTGA 4 cut(s) 141, 488, 1424, 1861
Hpy166II GTNNAC 3 cut(s) 503, 859, 1074
Hpy8I GTNNAC 3 cut(s) 503, 859, 1074
Hpy99I CGWCG 7 cut(s) 61, 64, 73, 76, 164, 892, 1133
HpyAV CCTTC 3 cut(s) 860, 986, 1450
HpyCH4III ACNGT 3 cut(s) 500, 804, 1723
HpyCH4IV ACGT 7 cut(s) 59, 481, 718, 887, 946, 1192, 1429
HpyF10VI GCNNNNNNNGC 3 cut(s) 355, 440, 446
HpyF3I CTNAG 8 cut(s) 339, 579, 784, 835, 875, 987, 1309, 1765
HpySE526I ACGT 7 cut(s) 59, 481, 718, 887, 946, 1192, 1429
Hsp92I GRCGYC 1 cut(s) 59
HspAI GCGC 3 cut(s) 8, 531, 1444
Kzo9I GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
LguI GCTCTTC 3 cut(s) 125, 519, 960
LmnI GCTCC 2 cut(s) 308, 836
Lsp1109I GCAGC 4 cut(s) 427, 1415, 1453, 1693
LweI GCATC 5 cut(s) 436, 1318, 1459, 1601, 1769
MabI ACCWGGT 1 cut(s) 45
MaeI CTAG 3 cut(s) 950, 1280, 1668
MaeII ACGT 7 cut(s) 59, 481, 718, 887, 946, 1192, 1429
MaeIII GTNAC 4 cut(s) 226, 736, 942, 1430
MalI GATC 6 cut(s) 221, 261, 466, 493, 1605, 1793
MbiI CCGCTC 1 cut(s) 512
MboI GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
MfeI CAATTG 2 cut(s) 429, 450
MflI RGATCY 3 cut(s) 259, 491, 1603
MlsI TGGCCA 2 cut(s) 353, 1782
MluNI TGGCCA 2 cut(s) 353, 1782
MlyI GAGTC 3 cut(s) 676, 979, 1022
MmeI TCCRAC 3 cut(s) 168, 786, 1459
Mox20I TGGCCA 2 cut(s) 353, 1782
MscI TGGCCA 2 cut(s) 353, 1782
MseI TTAA 7 cut(s) 540, 708, 1103, 1137, 1425, 1704, 1864
Msp20I TGGCCA 2 cut(s) 353, 1782
MspA1I CMGCKG 1 cut(s) 1563
MspR9I CCNGG 2 cut(s) 33, 47
MunI CAATTG 2 cut(s) 429, 450
Mva1269I GAATGC 3 cut(s) 456, 592, 967
MvaI CCWGG 2 cut(s) 33, 47
MvnI CGCG 1 cut(s) 1446
MwoI GCNNNNNNNGC 3 cut(s) 355, 440, 446
NdeII GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
NlaIV GGNNCC 5 cut(s) 93, 576, 1517, 1605, 1638
NmuCI GTSAC 1 cut(s) 1430
PagI TCATGA 4 cut(s) 375, 484, 1233, 1788
PciSI GCTCTTC 3 cut(s) 125, 519, 960
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 68
PctI GAATGC 3 cut(s) 456, 592, 967
PfeI GAWTC 4 cut(s) 131, 155, 839, 1121
PflFI GACNNNGTC 2 cut(s) 58, 61
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 4 cut(s) 442, 1405, 1443, 1683
PleI GAGTC 3 cut(s) 676, 979, 1022
PpsI GAGTC 3 cut(s) 676, 979, 1022
Ppu21I YACGTR 1 cut(s) 1193
PsiI TTATAA 1 cut(s) 726
Psp6I CCWGG 2 cut(s) 31, 45
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 2 cut(s) 31, 45
PspN4I GGNNCC 5 cut(s) 93, 576, 1517, 1605, 1638
PspPI GGNCC 5 cut(s) 277, 544, 847, 871, 1515
PstNI CAGNNNCTG 3 cut(s) 440, 1031, 1217
PsuI RGATCY 3 cut(s) 259, 491, 1603
PsyI GACNNNGTC 2 cut(s) 58, 61
PvuII CAGCTG 1 cut(s) 1563
RsaI GTAC 2 cut(s) 77, 801
RsaNI GTAC 2 cut(s) 76, 800
SapI GCTCTTC 3 cut(s) 125, 519, 960
SaqAI TTAA 7 cut(s) 540, 708, 1103, 1137, 1425, 1704, 1864
SatI GCNGC 4 cut(s) 441, 1404, 1442, 1682
Sau3AI GATC 6 cut(s) 219, 259, 464, 491, 1603, 1791
Sau96I GGNCC 5 cut(s) 277, 544, 847, 871, 1515
SchI GAGTC 3 cut(s) 676, 979, 1022
ScrFI CCNGG 2 cut(s) 33, 47
SexAI ACCWGGT 1 cut(s) 45
SfaNI GCATC 5 cut(s) 436, 1318, 1459, 1601, 1769
SinI GGWCC 3 cut(s) 277, 847, 1515
SmlI CTYRAG 2 cut(s) 702, 1415
SmoI CTYRAG 2 cut(s) 702, 1415
SnaBI TACGTA 1 cut(s) 1193
SsiI CCGC 4 cut(s) 168, 306, 510, 1847
SspMI CTAG 3 cut(s) 950, 1280, 1668
StyD4I CCNGG 2 cut(s) 31, 45
TaaI ACNGT 3 cut(s) 500, 804, 1723
TaiI ACGT 7 cut(s) 62, 484, 721, 890, 949, 1195, 1432
TaqI TCGA 3 cut(s) 222, 243, 1166
TatI WGTACW 1 cut(s) 799
TfiI GAWTC 4 cut(s) 131, 155, 839, 1121
Tru1I TTAA 7 cut(s) 540, 708, 1103, 1137, 1425, 1704, 1864
Tru9I TTAA 7 cut(s) 540, 708, 1103, 1137, 1425, 1704, 1864
TscAI CASTG 4 cut(s) 474, 613, 1458, 1526
TseFI GTSAC 1 cut(s) 1430
TseI GCWGC 4 cut(s) 440, 1403, 1441, 1681
Tsp45I GTSAC 1 cut(s) 1430
TspDTI ATGAA 4 cut(s) 645, 831, 1250, 1305
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 4 cut(s) 474, 613, 1458, 1526
Tth111I GACNNNGTC 2 cut(s) 58, 61
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 277, 847, 1515
XapI RAATTY 4 cut(s) 246, 920, 1237, 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1526
XspI CTAG 3 cut(s) 950, 1280, 1668
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.