FvH4_5g29960

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001924
Physical Location & Seq
Forward (+)
20831572 .. 20840474
8903 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29960.t2

Sequence Viewer

Length: 1929 bp
ATGGAATCGATTCTAGGGTTTATGGAAGAAGAAGACCCGGTGGAAACGTCGTCGTCTTCGTCTCCTTACAGCCACAAACTGGTGGCGTGGTTGAGCTGGGACGAGTGGCTCTTCGTCTCCCAAGCTCTCTTTTCCGATTCACCTGATTCCGTCGCCTCCGCTTTGAGAAGAATATCGGCATGGAAAAGCAGAGGATGTGTTCCTGCTGTTATCGAAGTTACTGCTTCCATTATCGAAATTCATCAAAAAGATCCTCAGTTTAGAAAGGACCACTCAGATGATGCTGTGGATAATTGCAGAGCAGATCAATGCACTGATTCGGAACTTTCTGAGGAAATGCTGGCCATGCTATATTGCATGACTATCATGAGGCTTGTGAATTGTGTTGTTGAGAAGACACGCAAGAAAAATGAGATTTCAATTGCTGAGGCTGCTGATGCAATTGGCATTCCACGATCACTAATTGATATTCGTCATGAGGGATCTCACCGTGAACTTCCCGCTCTTGAGGTTGTTCGTAGTGCTTCCATGAAGGCCCTTGATTGGTTGAAGTATTATTATTGGGAACCTCAGAAGAATGCAATTCCATTCCAAGGTACTGGAAATGCTAACATCAGAAATGAAATAAAGTCAAAGTTTCATGAATTGGCTTTCTGCTTGAGAGCTAAAATGACGCGATCTTCTCAAGTCAAAGAAAAACATGGTGGCAGACATGGTAAATGGCTTTGTGGGCGTAGTACATTTTTCTCAATAATGGCTGGTTATAAAAAAGATGTTACCAAGATAGTGAAAGGTCTTGTTGGGTTGTATTCGTCTTTCTCGTCAGAAGTTGTATCTGTACTGTTGGAGCTTTTGTTGAACACTAAGAGTTCTTCAGATTCATTGGACCTTCCAGAAAACACCCGAAAGGACCCAAGCATATATACGTCATTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCCAATAAGAAACCAGAGTTACGTCTAGCTCTTCTTAATGCAGTTCTTGATATGATTGAGACTCAGGAAGAAATGATGGGAAGAGGCTTGAACACATCATCAGACTGTGGGGCAAAGATATACCAAGTTGAACATCTCTCTTCTCTGTTTACATGGCTTGTTAGAAGTTTCAAAGGATTAAAGCCTCCTGAAGAAGATTCTGCTGACGAGGTTAAAGTTTCTTCAGCGGAAAAAACTATCTCGAAAGCAGTTCTGATGGATGTCCTGCGTAAATGTCTTCTTGTATCAGCTTCTGCTAATAACCATCTCATGAAGTCTGCCCTCCATCTTGCAAAGCTTACAGGAGACGAGTACCTAGTGCAAAAACTCAATAAACTCTCTTTCTTAGTTTCATCAAATTTGGATGCTGCTAAAGAAAACACTTGTCTTGCAAGTTCAAATAGTATATTTGTCCAGCAAGAAAAATCTATAAGTCAAGCAGCAAAGAAGCTAGAGTTGATTAAACGTCACCAAATGAAGCGCGAAGCAGTGAAGGGAACAAGAGATGCTGATGTTGGAAGTTCTAAAAGATGGGTTATGGCAAAATCATGGACCCGGTGCCCTATTGGAATGTTGCCTTGTGCTGTTGGGCATTCCGGCAGCCTGCCTATTCTGGACTATAATGGTGCATCTGAACAGGATCCTGAAGTACCTGGAGAAAACGAGAAGTGGGAACTGAACTCGTGTACTGGCAAGAAACGAGCTAATTCCGATATGCAGCTATTCGATAACCCATGTGCTAAAAAGATGAGGGGGACAGTGGTAGATGATTATGTATCAGACATGGAAGATGTTTTGTCCTCAGAAGAGGTCAATGCCATGTCCTCAGAAGATGTCTGTGGCCATCTCATGATTGGTGGTGTCTGGAAGAAAGTTGGGGAGGAAGAAATACTTGCCATCAAGTCTGCTGTGAGGATATTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

643

Amino Acids

71.62

Weight (kDa)

6.44

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 28 - 190 2.4e-43 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 765
AccB1I GGYRCC 1 cut(s) 1560
AccB7I CCANNNNNTGG 1 cut(s) 79
AccBSI CCGCTC 1 cut(s) 503
AccII CGCG 2 cut(s) 676, 1485
AciI CCGC 3 cut(s) 159, 501, 1190
AclWI GGATC 4 cut(s) 245, 490, 1637, 1650
AcoI YGGCCR 2 cut(s) 342, 1843
AcsI RAATTY 3 cut(s) 237, 959, 1360
AcuI CTGAAG 4 cut(s) 858, 1170, 1173, 1668
AfaI GTAC 6 cut(s) 598, 739, 840, 1316, 1653, 1690
AfiI CCNNNNNNNGG 3 cut(s) 79, 543, 593
AgsI TTSAA 8 cut(s) 420, 550, 859, 934, 1054, 1094, 1135, 1401
AjnI CCWGG 1 cut(s) 1654
Alw26I GTCTC 4 cut(s) 66, 121, 1016, 1302
AlwI GGATC 4 cut(s) 245, 490, 1637, 1650
AlwNI CAGNNNCTG 1 cut(s) 1256
AoxI GGCC 3 cut(s) 342, 534, 1843
ApeKI GCWGC 5 cut(s) 431, 1370, 1442, 1602, 1720
ApoI RAATTY 3 cut(s) 237, 959, 1360
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 1485
AspS9I GGNCC 5 cut(s) 268, 535, 886, 910, 1554
AsuC2I CCSGG 2 cut(s) 38, 1558
AsuHPI GGTGA 3 cut(s) 132, 479, 1463
AvaII GGWCC 4 cut(s) 268, 886, 910, 1554
BaeGI GKGCMC 1 cut(s) 1565
BalI TGGCCA 2 cut(s) 344, 1845
BamHI GGATCC 1 cut(s) 1642
BanI GGYRCC 1 cut(s) 1560
BauI CACGAG 1 cut(s) 1684
BbsI GAAGAC 4 cut(s) 39, 48, 401, 1232
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 5 cut(s) 418, 1357, 1454, 1614, 1732
BccI CCATC 7 cut(s) 1033, 1213, 1275, 1296, 1527, 1854, 1907
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 1 cut(s) 1656
BcnI CCSGG 2 cut(s) 38, 1558
BcoDI GTCTC 4 cut(s) 66, 121, 1016, 1302
BfaI CTAG 4 cut(s) 14, 989, 1319, 1454
BisI GCNGC 5 cut(s) 432, 1371, 1443, 1603, 1721
BlsI GCNGC 5 cut(s) 433, 1372, 1444, 1604, 1722
Bme1390I CCNGG 3 cut(s) 38, 1558, 1656
Bme18I GGWCC 4 cut(s) 268, 886, 910, 1554
BmgT120I GGNCC 5 cut(s) 268, 535, 886, 910, 1554
BmiI GGNNCC 5 cut(s) 567, 912, 1556, 1562, 1644
BmrFI CCNGG 3 cut(s) 38, 1558, 1656
BmsI GCATC 5 cut(s) 271, 427, 1357, 1498, 1640
BpiI GAAGAC 4 cut(s) 39, 48, 401, 1232
BpmI CTGGAG 1 cut(s) 1677
Bpu10I CCTNAGC 1 cut(s) 426
BpuEI CTTGAG 3 cut(s) 527, 669, 679
BpuMI CCSGG 2 cut(s) 38, 1558
Bsa29I ATCGAT 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 592
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 543, 593
Bse1I ACTGG 3 cut(s) 84, 604, 1696
BseBI CCWGG 1 cut(s) 1656
BseCI ATCGAT 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 592
BseGI GGATG 3 cut(s) 200, 1228, 1372
BseLI CCNNNNNNNGG 3 cut(s) 79, 543, 593
BseMII CTCAG 8 cut(s) 269, 288, 321, 417, 584, 1040, 1818, 1842
BseNI ACTGG 3 cut(s) 84, 604, 1696
BseSI GKGCMC 1 cut(s) 1565
BseXI GCAGC 5 cut(s) 418, 1357, 1454, 1614, 1732
BseYI CCCAGC 1 cut(s) 96
Bsh1236I CGCG 2 cut(s) 676, 1485
BshFI GGCC 3 cut(s) 344, 536, 1845
BshNI GGYRCC 1 cut(s) 1560
BshVI ATCGAT 1 cut(s) 8
BsiSI CCGG 3 cut(s) 38, 1558, 1599
BslFI GGGAC 2 cut(s) 113, 1771
BslI CCNNNNNNNGG 3 cut(s) 79, 543, 593
BsmAI GTCTC 4 cut(s) 66, 121, 1016, 1302
BsmBI CGTCTC 3 cut(s) 66, 121, 1302
BsmFI GGGAC 2 cut(s) 113, 1771
BsmI GAATGC 3 cut(s) 447, 583, 1594
BsnI GGCC 3 cut(s) 344, 536, 1845
Bsp1286I GDGCHC 1 cut(s) 1565
Bsp143I GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
BspACI CCGC 3 cut(s) 159, 501, 1190
BspANI GGCC 3 cut(s) 344, 536, 1845
BspCNI CTCAG 8 cut(s) 268, 287, 322, 418, 583, 1039, 1817, 1841
BspDI ATCGAT 1 cut(s) 8
BspFNI CGCG 2 cut(s) 676, 1485
BspHI TCATGA 5 cut(s) 366, 475, 640, 1272, 1851
BspLI GGNNCC 5 cut(s) 567, 912, 1556, 1562, 1644
BspPI GGATC 4 cut(s) 245, 490, 1637, 1650
BspQI GCTCTTC 2 cut(s) 116, 999
BspT107I GGYRCC 1 cut(s) 1560
BsrBI CCGCTC 1 cut(s) 503
BsrI ACTGG 3 cut(s) 84, 604, 1696
BssECI CCNNGG 1 cut(s) 592
BssMI GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
BssSI CACGAG 1 cut(s) 1684
BssT1I CCWWGG 1 cut(s) 592
Bst2BI CACGAG 1 cut(s) 1684
Bst2UI CCWGG 1 cut(s) 1656
Bst4CI ACNGT 4 cut(s) 491, 843, 1070, 1762
Bst6I CTCTTC 5 cut(s) 116, 999, 1039, 1108, 1803
BstC8I GCNNGC 2 cut(s) 342, 1607
BstF5I GGATG 3 cut(s) 200, 1228, 1372
BstFNI CGCG 2 cut(s) 676, 1485
BstHHI GCGC 1 cut(s) 1485
BstKTI GATC 6 cut(s) 253, 307, 458, 485, 680, 1645
BstMAI GTCTC 4 cut(s) 66, 121, 1016, 1302
BstMBI GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
BstMWI GCNNNNNNNGC 4 cut(s) 346, 431, 437, 730
BstNI CCWGG 1 cut(s) 1656
BstSCI CCNGG 3 cut(s) 36, 1556, 1654
BstSLI GKGCMC 1 cut(s) 1565
BstUI CGCG 2 cut(s) 676, 1485
BstV1I GCAGC 5 cut(s) 418, 1357, 1454, 1614, 1732
BstV2I GAAGAC 4 cut(s) 39, 48, 401, 1232
BstX2I RGATCY 3 cut(s) 250, 482, 1642
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 3 cut(s) 250, 482, 1642
Bsu15I ATCGAT 1 cut(s) 8
BsuRI GGCC 3 cut(s) 344, 536, 1845
BsuTUI ATCGAT 1 cut(s) 8
BtsCI GGATG 3 cut(s) 200, 1228, 1372
BtsI GCAGTG 1 cut(s) 1497
BtsIMutI CAGTG 3 cut(s) 312, 1497, 1767
Cac8I GCNNGC 2 cut(s) 342, 1607
CaiI CAGNNNCTG 1 cut(s) 1256
CciI TCATGA 5 cut(s) 366, 475, 640, 1272, 1851
CfoI GCGC 1 cut(s) 1485
Cfr13I GGNCC 5 cut(s) 268, 535, 886, 910, 1554
ClaI ATCGAT 1 cut(s) 8
CseI GACGC 1 cut(s) 682
Csp6I GTAC 6 cut(s) 597, 738, 839, 1315, 1652, 1689
CviQI GTAC 6 cut(s) 597, 738, 839, 1315, 1652, 1689
DpnI GATC 6 cut(s) 252, 306, 457, 484, 679, 1644
DpnII GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
EaeI YGGCCR 2 cut(s) 342, 1843
Eam1104I CTCTTC 5 cut(s) 116, 999, 1039, 1108, 1803
EarI CTCTTC 5 cut(s) 116, 999, 1039, 1108, 1803
Eco130I CCWWGG 1 cut(s) 592
Eco47I GGWCC 4 cut(s) 268, 886, 910, 1554
Eco57I CTGAAG 4 cut(s) 858, 1170, 1173, 1668
EcoO109I RGGNCCY 2 cut(s) 535, 910
EcoRII CCWGG 1 cut(s) 1654
EcoT14I CCWWGG 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 592
Esp3I CGTCTC 3 cut(s) 66, 121, 1302
FalI AAGNNNNNCTT 2 cut(s) 1878, 1910
FaqI GGGAC 2 cut(s) 113, 1771
FauI CCCGC 1 cut(s) 508
Fnu4HI GCNGC 5 cut(s) 432, 1371, 1443, 1603, 1721
FokI GGATG 3 cut(s) 207, 1235, 1379
Fsp4HI GCNGC 5 cut(s) 432, 1371, 1443, 1603, 1721
FspBI CTAG 4 cut(s) 14, 989, 1319, 1454
GlaI GCGC 1 cut(s) 1484
GluI GCNGC 5 cut(s) 432, 1371, 1443, 1603, 1721
GsaI CCCAGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 1677
HaeIII GGCC 3 cut(s) 344, 536, 1845
HapII CCGG 3 cut(s) 38, 1558, 1599
HgaI GACGC 1 cut(s) 682
HhaI GCGC 1 cut(s) 1485
Hin6I GCGC 1 cut(s) 1483
HinP1I GCGC 1 cut(s) 1483
HindIII AAGCTT 2 cut(s) 944, 1298
HinfI GANTC 8 cut(s) 5, 10, 137, 146, 317, 878, 1024, 1160
HpaII CCGG 3 cut(s) 38, 1558, 1599
HphI GGTGA 3 cut(s) 132, 479, 1463
Hpy166II GTNNAC 3 cut(s) 494, 1113, 1689
Hpy8I GTNNAC 3 cut(s) 494, 1113, 1689
Hpy99I CGWCG 3 cut(s) 52, 55, 155
HpyAV CCTTC 3 cut(s) 526, 899, 1489
HpyCH4III ACNGT 4 cut(s) 491, 843, 1070, 1762
HpyCH4IV ACGT 4 cut(s) 47, 926, 985, 1468
HpyF10VI GCNNNNNNNGC 4 cut(s) 346, 431, 437, 730
HpySE526I ACGT 4 cut(s) 47, 926, 985, 1468
HspAI GCGC 1 cut(s) 1483
Kzo9I GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
LguI GCTCTTC 2 cut(s) 116, 999
LmnI GCTCC 1 cut(s) 847
Lsp1109I GCAGC 5 cut(s) 418, 1357, 1454, 1614, 1732
LweI GCATC 5 cut(s) 271, 427, 1357, 1498, 1640
MaeI CTAG 4 cut(s) 14, 989, 1319, 1454
MaeII ACGT 4 cut(s) 47, 926, 985, 1468
MaeIII GTNAC 4 cut(s) 217, 775, 981, 1469
MalI GATC 6 cut(s) 252, 306, 457, 484, 679, 1644
MbiI CCGCTC 1 cut(s) 503
MboI GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
MfeI CAATTG 2 cut(s) 420, 441
MflI RGATCY 3 cut(s) 250, 482, 1642
MhlI GDGCHC 1 cut(s) 1565
MlsI TGGCCA 2 cut(s) 344, 1845
MluNI TGGCCA 2 cut(s) 344, 1845
MlyI GAGTC 1 cut(s) 1018
MmeI TCCRAC 2 cut(s) 825, 1498
Mox20I TGGCCA 2 cut(s) 344, 1845
MroXI GAANNNNTTC 1 cut(s) 9
MscI TGGCCA 2 cut(s) 344, 1845
MseI TTAA 4 cut(s) 999, 1142, 1176, 1464
MslI CAYNNNNRTG 1 cut(s) 276
Msp20I TGGCCA 2 cut(s) 344, 1845
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 3 cut(s) 38, 1558, 1599
MspR9I CCNGG 3 cut(s) 38, 1558, 1656
MunI CAATTG 2 cut(s) 420, 441
Mva1269I GAATGC 3 cut(s) 447, 583, 1594
MvaI CCWGG 1 cut(s) 1656
MvnI CGCG 2 cut(s) 676, 1485
MwoI GCNNNNNNNGC 4 cut(s) 346, 431, 437, 730
NciI CCSGG 2 cut(s) 38, 1558
NdeII GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
NlaIV GGNNCC 5 cut(s) 567, 912, 1556, 1562, 1644
NmuCI GTSAC 1 cut(s) 1469
PagI TCATGA 5 cut(s) 366, 475, 640, 1272, 1851
PciSI GCTCTTC 2 cut(s) 116, 999
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 818
PctI GAATGC 3 cut(s) 447, 583, 1594
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 7 cut(s) 5, 10, 137, 146, 317, 878, 1160
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 5 cut(s) 433, 1372, 1444, 1604, 1722
PleI GAGTC 1 cut(s) 1018
PpsI GAGTC 1 cut(s) 1018
PpuMI RGGWCCY 1 cut(s) 910
PsiI TTATAA 1 cut(s) 765
Psp5II RGGWCCY 1 cut(s) 910
Psp6I CCWGG 1 cut(s) 1654
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 1 cut(s) 1654
PspN4I GGNNCC 5 cut(s) 567, 912, 1556, 1562, 1644
PspPI GGNCC 5 cut(s) 268, 535, 886, 910, 1554
PspPPI RGGWCCY 1 cut(s) 910
PstNI CAGNNNCTG 1 cut(s) 1256
PsuI RGATCY 3 cut(s) 250, 482, 1642
RsaI GTAC 6 cut(s) 598, 739, 840, 1316, 1653, 1690
RsaNI GTAC 6 cut(s) 597, 738, 839, 1315, 1652, 1689
RseI CAYNNNNRTG 1 cut(s) 276
SapI GCTCTTC 2 cut(s) 116, 999
SaqAI TTAA 4 cut(s) 999, 1142, 1176, 1464
SatI GCNGC 5 cut(s) 432, 1371, 1443, 1603, 1721
Sau3AI GATC 6 cut(s) 250, 304, 455, 482, 677, 1642
Sau96I GGNCC 5 cut(s) 268, 535, 886, 910, 1554
SchI GAGTC 1 cut(s) 1018
ScrFI CCNGG 3 cut(s) 38, 1558, 1656
SduI GDGCHC 1 cut(s) 1565
SfaNI GCATC 5 cut(s) 271, 427, 1357, 1498, 1640
SinI GGWCC 4 cut(s) 268, 886, 910, 1554
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 506, 658, 684
SmoI CTYRAG 3 cut(s) 506, 658, 684
SsiI CCGC 3 cut(s) 159, 501, 1190
SspMI CTAG 4 cut(s) 14, 989, 1319, 1454
StyD4I CCNGG 3 cut(s) 36, 1556, 1654
StyI CCWWGG 1 cut(s) 592
TaaI ACNGT 4 cut(s) 491, 843, 1070, 1762
TaiI ACGT 4 cut(s) 50, 929, 988, 1471
TaqI TCGA 5 cut(s) 8, 213, 234, 1205, 1728
TatI WGTACW 3 cut(s) 737, 838, 1688
TfiI GAWTC 7 cut(s) 5, 10, 137, 146, 317, 878, 1160
Tru1I TTAA 4 cut(s) 999, 1142, 1176, 1464
Tru9I TTAA 4 cut(s) 999, 1142, 1176, 1464
TscAI CASTG 3 cut(s) 319, 1497, 1767
TseFI GTSAC 1 cut(s) 1469
TseI GCWGC 5 cut(s) 431, 1370, 1442, 1602, 1720
Tsp45I GTSAC 1 cut(s) 1469
TspDTI ATGAA 9 cut(s) 230, 545, 629, 636, 657, 870, 1289, 1344, 1493
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 3 cut(s) 319, 1497, 1767
Van91I CCANNNNNTGG 1 cut(s) 79
VpaK11BI GGWCC 4 cut(s) 268, 886, 910, 1554
XapI RAATTY 3 cut(s) 237, 959, 1360
XcmI CCANNNNNNNNNTGG 1 cut(s) 1853
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 4 cut(s) 14, 989, 1319, 1454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.