AT5G13250

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006209
Physical Location & Seq
Forward (+)
4234156 .. 4241934
7779 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13250.1

Sequence Viewer

Length: 1164 bp
ATGGTTTCTGCTGCTGAATCCTCTTCCTCCACGTCAACGCTTCTTCTTCCATGTGCGTTTGCCAACAGTTTTGCTTTTATGAGGTTATTACAAAAAACTCATCACTTCTCTGCCGTCTCTGAGGACGACGACAACAACAAAGACATTCACTTAGGATCTGATCATCATCAGGCAAAAGATAATAATCATCACCAAGTTTTTAGGAGAGAGTGTAGTTTCATGGCCGATCAAGAAGAGCCGAGGAGTACTACTTCTAGGTTAAGCTCCAAGGAGGACGACCACGACAACAATAGCAATAACAACAATATTAATAATAATAACAACAACGAAGCCGAAGATCATGAGATGAGACAACAAGGGTGGCTCCGGTTAAGCTTAGGTCACGAGGAGGACGTTAAACCGGATCTCGACCATCGGCAGCAACATCAAACTGATCCAACGGCTAGGAGAGACTCTTTTCTAGAGCTTAACCTTTCCTCTGGCGGTTCAAATAGAGAAGAAGAAGTTGGTCTTCCATTGTCATCTCTCTTTCATCATCAGCATCAAGCCGGAGGGATGATGATTAATCCATTGATGTTCCATACAAGGCCTCAGGATATGATTGGTCCTTGGGCGGCGGCTGCGTTTAGAACGCCTTTTGTCCCACAAAACCTAACACAACCATCATCATCATCATCTGGTTCTTTGATGATGCCATTGATGGGACAGTACTTTGGTCGCTCTAGTTTTCAGCCGCAGTTAATAGGGAATGATAACCCGGATGGGGTGGCAGGTCCAAGCTCGAGTTTAAGAGTCATTGATCCTCCCAGACGACCCCATTCCGGCATTTGGTTTCTCCTTCAAGCTTCTCAAAACCAGACAGTAGAACCATTCTTGCCTCAGATTCCAAAGAGCTACTTGAGAATCAAGGACGGGAAGATGACTGTTCGACTGTTGATGAAATATTTGGTGAATAAGTTGCGATTGGAGCACGAATCCCAGCAGGTAGAGATAAGATGCAGAGGGCAAGAACTGGAGCCAGTGTTGACACTACAACACGTGAGAGATGCTATATGGAGAGGAAGCAGAGACACTTCTTCTCTTTCACAAAACATTACTTTGCTTCCAAACTCATCTACCTCTGATCATCTCATGGTCCTCCATTATGGTAGGACTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

387

Amino Acids

43.74

Weight (kDa)

6.15

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3337 PF11816 276 - 357 2.7e-06 Domain of unknown function (DUF3337)
RAWUL PF16207 306 - 353 1.5e-06 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 761, 973
AciI CCGC 4 cut(s) 483, 614, 617, 734
AclWI GGATC 4 cut(s) 163, 411, 428, 794
AcoI YGGCCR 1 cut(s) 222
AcvI CACGTG 1 cut(s) 1039
AfaI GTAC 2 cut(s) 247, 710
AfiI CCNNNNNNNGG 4 cut(s) 701, 763, 821, 828
AflIII ACRYGT 1 cut(s) 1036
AgsI TTSAA 2 cut(s) 489, 842
AjiI CACGTC 1 cut(s) 33
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 6 cut(s) 264, 375, 466, 780, 845, 894
AluI AGCT 6 cut(s) 264, 375, 466, 780, 845, 894
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 4 cut(s) 121, 343, 444, 1062
AlwI GGATC 4 cut(s) 163, 411, 428, 794
Ama87I CYCGRG 1 cut(s) 781
AoxI GGCC 2 cut(s) 222, 587
ApeKI GCWGC 3 cut(s) 11, 418, 620
AseI ATTAAT 2 cut(s) 309, 564
AspS9I GGNCC 3 cut(s) 605, 773, 1135
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 2 cut(s) 182, 961
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 3 cut(s) 605, 773, 1135
AxyI CCTNAGG 1 cut(s) 591
BauI CACGAG 1 cut(s) 383
BbrPI CACGTG 1 cut(s) 1039
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 2 cut(s) 430, 607
BccI CCATC 4 cut(s) 420, 670, 694, 755
BceAI ACGGC 2 cut(s) 98, 456
BclI TGATCA 2 cut(s) 160, 1123
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 4 cut(s) 121, 343, 444, 1062
BfaI CTAG 4 cut(s) 255, 444, 461, 723
BfuAI ACCTGC 2 cut(s) 761, 973
BisI GCNGC 6 cut(s) 12, 419, 615, 618, 621, 734
BlsI GCNGC 6 cut(s) 13, 420, 616, 619, 622, 735
BmcAI AGTACT 2 cut(s) 247, 710
Bme1390I CCNGG 1 cut(s) 758
Bme18I GGWCC 3 cut(s) 605, 773, 1135
BmeT110I CYCGRG 1 cut(s) 781
BmgBI CACGTC 1 cut(s) 33
BmgT120I GGNCC 3 cut(s) 605, 773, 1135
BmiI GGNNCC 2 cut(s) 365, 1017
BmrFI CCNGG 1 cut(s) 758
BmsI GCATC 4 cut(s) 550, 681, 986, 1036
BpiI GAAGAC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 1034
Bpu10I CCTNAGC 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 758
BsaAI YACGTR 1 cut(s) 1039
BsaBI GATNNNNATC 2 cut(s) 165, 183
BsaJI CCNNGG 3 cut(s) 239, 267, 608
BsaWI WCCGGW 2 cut(s) 366, 400
BsaXI ACNNNNNCTCC 4 cut(s) 196, 226, 1007, 1037
Bsc4I CCNNNNNNNGG 4 cut(s) 701, 763, 821, 828
Bse1I ACTGG 2 cut(s) 1017, 1019
Bse21I CCTNAGG 1 cut(s) 591
Bse8I GATNNNNATC 2 cut(s) 165, 183
BseDI CCNNGG 3 cut(s) 239, 267, 608
BseGI GGATG 2 cut(s) 561, 766
BseJI GATNNNNATC 2 cut(s) 165, 183
BseLI CCNNNNNNNGG 4 cut(s) 701, 763, 821, 828
BseMII CTCAG 3 cut(s) 111, 605, 893
BseNI ACTGG 2 cut(s) 1017, 1019
BseRI GAGGAG 2 cut(s) 256, 401
BseXI GCAGC 2 cut(s) 430, 607
BseYI CCCAGC 1 cut(s) 978
BshFI GGCC 2 cut(s) 224, 589
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 5 cut(s) 367, 401, 549, 758, 822
BslFI GGGAC 2 cut(s) 626, 717
BslI CCNNNNNNNGG 4 cut(s) 701, 763, 821, 828
BsmAI GTCTC 4 cut(s) 121, 343, 444, 1062
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 2 cut(s) 626, 717
BsnI GGCC 2 cut(s) 224, 589
BsoBI CYCGRG 1 cut(s) 781
Bsp1286I GDGCHC 1 cut(s) 972
Bsp143I GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
BspACI CCGC 4 cut(s) 483, 614, 617, 734
BspANI GGCC 2 cut(s) 224, 589
BspCNI CTCAG 3 cut(s) 112, 604, 892
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 365, 1017
BspMI ACCTGC 2 cut(s) 761, 973
BspPI GGATC 4 cut(s) 163, 411, 428, 794
BspQI GCTCTTC 1 cut(s) 228
BsrI ACTGG 2 cut(s) 1017, 1019
BssECI CCNNGG 3 cut(s) 239, 267, 608
BssMI GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
BssSI CACGAG 1 cut(s) 383
BssT1I CCWWGG 2 cut(s) 267, 608
Bst2BI CACGAG 1 cut(s) 383
Bst4CI ACNGT 5 cut(s) 68, 708, 862, 925, 933
Bst6I CTCTTC 2 cut(s) 28, 228
BstBAI YACGTR 1 cut(s) 1039
BstDEI CTNAG 5 cut(s) 120, 151, 376, 591, 879
BstF5I GGATG 2 cut(s) 561, 766
BstKTI GATC 8 cut(s) 158, 163, 229, 340, 406, 436, 802, 1126
BstMAI GTCTC 4 cut(s) 121, 343, 444, 1062
BstMBI GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
BstMWI GCNNNNNNNGC 2 cut(s) 620, 967
BstSCI CCNGG 1 cut(s) 756
BstV1I GCAGC 2 cut(s) 430, 607
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 2 cut(s) 155, 403
BstYI RGATCY 2 cut(s) 155, 403
Bsu36I CCTNAGG 1 cut(s) 591
BsuRI GGCC 2 cut(s) 224, 589
BtrI CACGTC 1 cut(s) 33
BtsCI GGATG 2 cut(s) 561, 766
BtsIMutI CAGTG 1 cut(s) 1026
BveI ACCTGC 2 cut(s) 761, 973
CciI TCATGA 1 cut(s) 340
Cfr13I GGNCC 3 cut(s) 605, 773, 1135
Csp6I GTAC 2 cut(s) 246, 709
CspCI CAANNNNNGTGG 2 cut(s) 341, 376
CviAII CATG 4 cut(s) 51, 220, 341, 1132
CviQI GTAC 2 cut(s) 246, 709
DdeI CTNAG 5 cut(s) 120, 151, 376, 591, 879
DpnI GATC 8 cut(s) 157, 162, 228, 339, 405, 435, 801, 1125
DpnII GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 28, 228
EarI CTCTTC 2 cut(s) 28, 228
Eco130I CCWWGG 2 cut(s) 267, 608
Eco147I AGGCCT 1 cut(s) 589
Eco47I GGWCC 3 cut(s) 605, 773, 1135
Eco72I CACGTG 1 cut(s) 1039
Eco81I CCTNAGG 1 cut(s) 591
Eco88I CYCGRG 1 cut(s) 781
EcoT14I CCWWGG 2 cut(s) 267, 608
ErhI CCWWGG 2 cut(s) 267, 608
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 4 cut(s) 54, 223, 344, 1135
FalI AAGNNNNNCTT 4 cut(s) 495, 527, 881, 913
FaqI GGGAC 2 cut(s) 626, 717
FatI CATG 4 cut(s) 50, 219, 340, 1131
FbaI TGATCA 2 cut(s) 160, 1123
Fnu4HI GCNGC 6 cut(s) 12, 419, 615, 618, 621, 734
FokI GGATG 2 cut(s) 568, 773
Fsp4HI GCNGC 6 cut(s) 12, 419, 615, 618, 621, 734
FspBI CTAG 4 cut(s) 255, 444, 461, 723
GluI GCNGC 6 cut(s) 12, 419, 615, 618, 621, 734
GsaI CCCAGC 1 cut(s) 982
GsuI CTGGAG 1 cut(s) 1034
HaeIII GGCC 2 cut(s) 224, 589
HapII CCGG 5 cut(s) 367, 401, 549, 758, 822
Hin1II CATG 4 cut(s) 54, 223, 344, 1135
HincII GTYRAC 2 cut(s) 36, 1026
HindII GTYRAC 2 cut(s) 36, 1026
HindIII AAGCTT 2 cut(s) 373, 843
HinfI GANTC 6 cut(s) 17, 452, 792, 883, 903, 974
HpaII CCGG 5 cut(s) 367, 401, 549, 758, 822
HphI GGTGA 2 cut(s) 182, 961
Hpy166II GTNNAC 2 cut(s) 36, 1026
Hpy188I TCNGA 4 cut(s) 121, 160, 882, 1123
Hpy188III TCNNGA 6 cut(s) 230, 341, 383, 407, 461, 593
Hpy8I GTNNAC 2 cut(s) 36, 1026
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 848
HpyCH4III ACNGT 5 cut(s) 68, 708, 862, 925, 933
HpyCH4IV ACGT 3 cut(s) 32, 393, 1038
HpyCH4V TGCA 1 cut(s) 999
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 967
HpyF3I CTNAG 5 cut(s) 120, 151, 376, 591, 879
HpySE526I ACGT 3 cut(s) 32, 393, 1038
Hsp92II CATG 4 cut(s) 54, 223, 344, 1135
Ksp22I TGATCA 2 cut(s) 160, 1123
Kzo9I GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 4 cut(s) 269, 369, 967, 1015
Lsp1109I GCAGC 2 cut(s) 430, 607
LweI GCATC 4 cut(s) 550, 681, 986, 1036
MaeI CTAG 4 cut(s) 255, 444, 461, 723
MaeII ACGT 3 cut(s) 32, 393, 1038
MaeIII GTNAC 1 cut(s) 380
MalI GATC 8 cut(s) 157, 162, 228, 339, 405, 435, 801, 1125
MboI GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
MflI RGATCY 2 cut(s) 155, 403
MhlI GDGCHC 1 cut(s) 972
MlyI GAGTC 2 cut(s) 446, 801
MmeI TCCRAC 1 cut(s) 461
MseI TTAA 9 cut(s) 260, 309, 371, 396, 468, 564, 740, 788, 1162
MspI CCGG 5 cut(s) 367, 401, 549, 758, 822
MspR9I CCNGG 1 cut(s) 758
MwoI GCNNNNNNNGC 2 cut(s) 620, 967
NciI CCSGG 1 cut(s) 758
NdeII GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
NlaIII CATG 4 cut(s) 54, 223, 344, 1135
NlaIV GGNNCC 2 cut(s) 365, 1017
NmeAIII GCCGAG 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 781
PagI TCATGA 1 cut(s) 340
PceI AGGCCT 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PfeI GAWTC 4 cut(s) 17, 883, 903, 974
PkrI GCNGC 6 cut(s) 13, 420, 616, 619, 622, 735
PleI GAGTC 2 cut(s) 446, 800
PmaCI CACGTG 1 cut(s) 1039
PmlI CACGTG 1 cut(s) 1039
PpsI GAGTC 2 cut(s) 446, 800
Ppu21I YACGTR 1 cut(s) 1039
PshBI ATTAAT 2 cut(s) 309, 564
PspCI CACGTG 1 cut(s) 1039
PspFI CCCAGC 1 cut(s) 978
PspN4I GGNNCC 2 cut(s) 365, 1017
PspPI GGNCC 3 cut(s) 605, 773, 1135
PspXI VCTCGAGB 1 cut(s) 781
PsuI RGATCY 2 cut(s) 155, 403
RsaI GTAC 2 cut(s) 247, 710
RsaNI GTAC 2 cut(s) 246, 709
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 9 cut(s) 260, 309, 371, 396, 468, 564, 740, 788, 1162
SatI GCNGC 6 cut(s) 12, 419, 615, 618, 621, 734
Sau3AI GATC 8 cut(s) 155, 160, 226, 337, 403, 433, 799, 1123
Sau96I GGNCC 3 cut(s) 605, 773, 1135
ScaI AGTACT 2 cut(s) 247, 710
SchI GAGTC 2 cut(s) 446, 801
ScrFI CCNGG 1 cut(s) 758
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 4 cut(s) 550, 681, 986, 1036
Sfr274I CTCGAG 1 cut(s) 781
SinI GGWCC 3 cut(s) 605, 773, 1135
SlaI CTCGAG 1 cut(s) 781
SmlI CTYRAG 2 cut(s) 781, 898
SmoI CTYRAG 2 cut(s) 781, 898
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 4 cut(s) 483, 614, 617, 734
SspI AATATT 2 cut(s) 307, 944
SspMI CTAG 4 cut(s) 255, 444, 461, 723
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 1 cut(s) 756
StyI CCWWGG 2 cut(s) 267, 608
TaaI ACNGT 5 cut(s) 68, 708, 862, 925, 933
TaiI ACGT 3 cut(s) 35, 396, 1041
TaqI TCGA 3 cut(s) 408, 782, 928
TatI WGTACW 2 cut(s) 245, 708
TauI GCSGC 3 cut(s) 617, 620, 736
TfiI GAWTC 4 cut(s) 17, 883, 903, 974
Tru1I TTAA 9 cut(s) 260, 309, 371, 396, 468, 564, 740, 788, 1162
Tru9I TTAA 9 cut(s) 260, 309, 371, 396, 468, 564, 740, 788, 1162
TscAI CASTG 1 cut(s) 1026
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 3 cut(s) 11, 418, 620
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 3 cut(s) 208, 521, 953
TspRI CASTG 1 cut(s) 1026
VpaK11BI GGWCC 3 cut(s) 605, 773, 1135
VspI ATTAAT 2 cut(s) 309, 564
XbaI TCTAGA 1 cut(s) 460
XhoI CTCGAG 1 cut(s) 781
XspI CTAG 4 cut(s) 255, 444, 461, 723
ZrmI AGTACT 2 cut(s) 247, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.