pycom13g11090

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006209
Physical Location & Seq
Forward (+)
7577904 .. 7586075
8172 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g11090.1

Sequence Viewer

Length: 720 bp
ATGCCTCCAATGTTTCATGTTCCTCAACACGAAAATATTTTCCGTGCTCCTCCTTCGAATCCGTCTTTCAGGCCTCCGTTTAATTCGAGTAACGCTACGGCTTTATACTTCCAGCAACCGGGGCCGGGAATGAGTAGCTCCTCGTCGGGTTTATTTCCTCACCAGGACGTTAACTGGGCATTTAGGCCAGCAATCCCTCATAATAATATAGGGATGACGGCTTCTACTTCATCTTCTTCTGCTTCCTCTTTTATACAACTGGGTTCATCCTATTTTGCACGCCCATTCCACCATCCTCTTCATGAATTTAACGTCGCCGGACCGAGCTTAGATTTCAGAGTTGTTGATCCTCCGAGAAGGCCCCAATCTGGCATTTGGTTTACACTCCAAGCATCCCAAAATCAAGCAAAAGAACCATTCTTGCCCCAGATACCCAAAAGCTACTTAAGAATCAAGGATGGAAGAATGACAGTTCGGTTGATAAAGAAGTATCTGGTTAATAAGCTGAGATTGGATAGCGAATCAGAGCAGGTAGAGATAACATGTAGAGGACAGGAGCTTCTACCTTTCTTGACGTTGCAGCACGTAAGAGACAACATCTGGAGTCTAAGGGACGCACTCACCTTGCTTCCAGAGTCCTCAACCACTGATCATGTCATGGTGTTGAACTATGCTAGGACCACGACGACGACAAAGACGACGACTACTACAACCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

26.87

Weight (kDa)

9.71

Isoelectric Point (pI)

65.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 155 - 206 2.6e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 520
AciI CCGC 1 cut(s) 714
AclWI GGATC 1 cut(s) 341
AcsI RAATTY 1 cut(s) 305
AfiI CCNNNNNNNGG 3 cut(s) 118, 125, 368
AflII CTTAAG 1 cut(s) 445
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 1 cut(s) 667
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 5 cut(s) 138, 327, 441, 505, 559
AluI AGCT 5 cut(s) 138, 327, 441, 505, 559
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 1 cut(s) 341
AoxI GGCC 4 cut(s) 71, 122, 185, 359
ApeKI GCWGC 1 cut(s) 580
ApoI RAATTY 1 cut(s) 305
AspS9I GGNCC 4 cut(s) 122, 320, 360, 678
AsuC2I CCSGG 2 cut(s) 120, 126
AsuHPI GGTGA 2 cut(s) 152, 613
AsuII TTCGAA 1 cut(s) 56
AvaII GGWCC 2 cut(s) 320, 678
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 592
BccI CCATC 2 cut(s) 300, 452
BceAI ACGGC 2 cut(s) 114, 234
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 2 cut(s) 120, 126
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 1 cut(s) 675
BfrI CTTAAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 1 cut(s) 581
BlsI GCNGC 1 cut(s) 582
Bme1390I CCNGG 3 cut(s) 120, 126, 164
Bme18I GGWCC 2 cut(s) 320, 678
BmgT120I GGNCC 4 cut(s) 122, 320, 360, 678
BmiI GGNNCC 2 cut(s) 123, 362
BmrFI CCNGG 3 cut(s) 120, 126, 164
BmrI ACTGGG 2 cut(s) 184, 269
BmsI GCATC 1 cut(s) 401
BmuI ACTGGG 2 cut(s) 184, 269
BpmI CTGGAG 1 cut(s) 622
Bpu14I TTCGAA 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 120, 126
BsaAI YACGTR 1 cut(s) 586
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 125, 368
Bse1I ACTGG 2 cut(s) 179, 264
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 5 cut(s) 219, 266, 292, 392, 463
BseLI CCNNNNNNNGG 3 cut(s) 118, 125, 368
BseMII CTCAG 1 cut(s) 497
BseNI ACTGG 2 cut(s) 179, 264
BseRI GAGGAG 2 cut(s) 39, 130
BseXI GCAGC 1 cut(s) 592
BshFI GGCC 4 cut(s) 73, 124, 187, 361
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 3 cut(s) 119, 125, 318
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 3 cut(s) 118, 125, 368
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 4 cut(s) 73, 124, 187, 361
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 346, 649
BspACI CCGC 1 cut(s) 714
BspANI GGCC 4 cut(s) 73, 124, 187, 361
BspCNI CTCAG 1 cut(s) 498
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 123, 362
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 56
BspTI CTTAAG 1 cut(s) 445
BsrI ACTGG 2 cut(s) 179, 264
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 2 cut(s) 346, 649
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 303
BstAFI CTTAAG 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 189, 280
BstDEI CTNAG 3 cut(s) 328, 506, 608
BstF5I GGATG 5 cut(s) 219, 266, 292, 392, 463
BstKTI GATC 2 cut(s) 349, 652
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 2 cut(s) 346, 649
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 3 cut(s) 118, 124, 162
BstV1I GCAGC 1 cut(s) 592
BsuRI GGCC 4 cut(s) 73, 124, 187, 361
BtsCI GGATG 5 cut(s) 219, 266, 292, 392, 463
BtsIMutI CAGTG 1 cut(s) 645
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 2 cut(s) 189, 280
CciI TCATGA 1 cut(s) 301
Cfr13I GGNCC 4 cut(s) 122, 320, 360, 678
CpoI CGGWCCG 1 cut(s) 320
CseI GACGC 1 cut(s) 623
CspI CGGWCCG 1 cut(s) 320
CviAII CATG 5 cut(s) 17, 302, 543, 653, 658
DdeI CTNAG 3 cut(s) 328, 506, 608
DpnI GATC 2 cut(s) 348, 651
DpnII GATC 2 cut(s) 346, 649
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 320, 678
EcoO109I RGGNCCY 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 162
FaeI CATG 5 cut(s) 20, 305, 546, 656, 661
FaqI GGGAC 1 cut(s) 626
FatI CATG 5 cut(s) 16, 301, 542, 652, 657
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 1 cut(s) 581
FokI GGATG 5 cut(s) 226, 253, 279, 379, 470
Fsp4HI GCNGC 1 cut(s) 581
FspBI CTAG 1 cut(s) 675
GluI GCNGC 1 cut(s) 581
GsuI CTGGAG 1 cut(s) 622
HaeIII GGCC 4 cut(s) 73, 124, 187, 361
HapII CCGG 3 cut(s) 119, 125, 318
HgaI GACGC 1 cut(s) 623
Hin1II CATG 5 cut(s) 20, 305, 546, 656, 661
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 58, 450, 521, 604, 635
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 3 cut(s) 119, 125, 318
HphI GGTGA 2 cut(s) 152, 613
Hpy166II GTNNAC 2 cut(s) 172, 381
Hpy188I TCNGA 3 cut(s) 338, 354, 526
Hpy188III TCNNGA 4 cut(s) 302, 571, 601, 632
Hpy8I GTNNAC 2 cut(s) 172, 381
Hpy99I CGWCG 5 cut(s) 148, 317, 688, 691, 703
HpyAV CCTTC 2 cut(s) 63, 351
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 4 cut(s) 168, 312, 575, 585
HpyCH4V TGCA 2 cut(s) 278, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 3 cut(s) 328, 506, 608
HpySE526I ACGT 4 cut(s) 168, 312, 575, 585
Hsp92II CATG 5 cut(s) 20, 305, 546, 656, 661
Ksp22I TGATCA 1 cut(s) 649
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 346, 649
LmnI GCTCC 3 cut(s) 52, 143, 556
Lsp1109I GCAGC 1 cut(s) 592
LweI GCATC 1 cut(s) 401
MaeI CTAG 1 cut(s) 675
MaeII ACGT 4 cut(s) 168, 312, 575, 585
MaeIII GTNAC 1 cut(s) 89
MalI GATC 2 cut(s) 348, 651
MboI GATC 2 cut(s) 346, 649
MboII GAAGA 4 cut(s) 225, 228, 290, 474
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 2 cut(s) 82, 305
MlyI GAGTC 2 cut(s) 613, 644
MseI TTAA 5 cut(s) 81, 171, 309, 446, 498
MspCI CTTAAG 1 cut(s) 445
MspI CCGG 3 cut(s) 119, 125, 318
MspR9I CCNGG 3 cut(s) 120, 126, 164
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 121
NciI CCSGG 2 cut(s) 120, 126
NdeII GATC 2 cut(s) 346, 649
NlaIII CATG 5 cut(s) 20, 305, 546, 656, 661
NlaIV GGNNCC 2 cut(s) 123, 362
NspI RCATGY 1 cut(s) 546
NspV TTCGAA 1 cut(s) 56
PagI TCATGA 1 cut(s) 301
PceI AGGCCT 1 cut(s) 73
PciI ACATGT 1 cut(s) 542
PcsI WCGNNNNNNNCGW 1 cut(s) 695
PfeI GAWTC 3 cut(s) 58, 450, 521
PkrI GCNGC 1 cut(s) 582
PleI GAGTC 2 cut(s) 612, 643
PpsI GAGTC 2 cut(s) 612, 643
Ppu21I YACGTR 1 cut(s) 586
PscI ACATGT 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 123, 362
PspPI GGNCC 4 cut(s) 122, 320, 360, 678
Rsr2I CGGWCCG 1 cut(s) 320
RsrII CGGWCCG 1 cut(s) 320
SaqAI TTAA 5 cut(s) 81, 171, 309, 446, 498
SatI GCNGC 1 cut(s) 581
Sau3AI GATC 2 cut(s) 346, 649
Sau96I GGNCC 4 cut(s) 122, 320, 360, 678
SchI GAGTC 2 cut(s) 613, 644
ScrFI CCNGG 3 cut(s) 120, 126, 164
SduI GDGCHC 1 cut(s) 49
SfaNI GCATC 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 2 cut(s) 320, 678
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
Sse9I AATT 2 cut(s) 82, 305
SseBI AGGCCT 1 cut(s) 73
SsiI CCGC 1 cut(s) 714
SspI AATATT 1 cut(s) 37
SspMI CTAG 1 cut(s) 675
StuI AGGCCT 1 cut(s) 73
StyD4I CCNGG 3 cut(s) 118, 124, 162
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 4 cut(s) 171, 315, 578, 588
TaqI TCGA 2 cut(s) 56, 86
TaqII GACCGA 1 cut(s) 337
TasI AATT 2 cut(s) 82, 305
TfiI GAWTC 3 cut(s) 58, 450, 521
Tru1I TTAA 5 cut(s) 81, 171, 309, 446, 498
Tru9I TTAA 5 cut(s) 81, 171, 309, 446, 498
TscAI CASTG 1 cut(s) 652
TseI GCWGC 1 cut(s) 580
TspDTI ATGAA 5 cut(s) 5, 219, 255, 290, 318
TspGWI ACGGA 3 cut(s) 32, 51, 66
TspRI CASTG 1 cut(s) 652
Vha464I CTTAAG 1 cut(s) 445
VpaK11BI GGWCC 2 cut(s) 320, 678
XapI RAATTY 1 cut(s) 305
XceI RCATGY 1 cut(s) 546
XspI CTAG 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.