RLG00000007087

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0006209
Physical Location & Seq
Forward (+)
13470134 .. 13477249
7116 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007087

Sequence Viewer

Length: 1332 bp
ATGACCATGGTTCCTTCTGCACATCATCAAAACTTATCCAGACAACAACAACATCCTCATCTCTACGGTGGTTATGGTGGCTGCGGTGCCAATTATTACAACAATACGGCGCCGGACGACGAGAGCTTTGGTGGTCATCTTATGAATCAAGTAGAGGAAGGTGGTTATGATCAGTATAGTTATTGTAATAACAATAATAATACTATTACTGATCAATCTTACTTTATAGCATCTTCCGATCTAGTAGTGGTTAATGGTCCGATGGCCGAAGATGAATCAAGAACTAACAGTCTTAACAACGAAGCCGAAGCCGAAGCGGGGTCAAGTTCTAAAGCTCCTGTAGATGAAGATCAGAGAGACGAGAGATGGCTCCAATTGAGTATAGGCGGCCAGAACGCTACCAGTGCAGACGTCCAAGCTCCGGAAAGAAGAAGCTCGTTAAGGGGTTCATCATCGGCATCCGGATTGATTGAGCTTGATCTGTTATCAGCAAATATATCGTCGCCGTCGTCGTCATCGTCACGGCAATTTCAGTTGCAACAAGCAGCCAGGTCTTCTATGACTCCCATGTTTCATGTTCCCGATCATCATCATCAAATTCGTGTTCCTAATTTCGGCACTAATTTGATGAACACTACGTCTAATTTATATTTTCAACACCCCGGAATGGGAATGGGAATGGGAATGAGTAGTTCTTTGCCGCCGAGCTTTCCTCAACACCAGCACCAGGAGGTTAACTGGGGATTTAGGCCATTGATCCCTCATCATAATAATCATATAGGGAATAGTGCTTCAACTTCTACCTCTTCCTCTTCTTTTATGCAACTGGGACCAGCATCCTCCTCCTATTTTGCACGCCCATTTCAACTTCAACATCATCATCTTCATCATGGTGGTGGTGCTGGGCCGAGCTTAGATTTCAGAGTTGTTGATCCTCCTCGGCGGCCCCAGTCTGGCATCTGGTTTATGCTACAAGCCTCACAAAATCAAGAAAAAGAACCGTTCTTGCCCCAGATACCGAAAAGCTACCTCAGGATCAAGGATGGAAGGATGACAGTTCGATTGATAAAGAAGTATCTGGTTAATAAGCTAAGACTCGACAGCGAATCAGAGGTAGAGATAACATGTCGAGGACAGCAGCTCCTACCTTTCTTGACGTTGCAGCATGTAAGAGATAATATATGGAGTCTAAGGGACGCAGCAGCAGCAGCAGCAGCATCACTCACTTTGCTTCCACTTCCAGATTCCTCCTCCTCCTCCACATCAACCACAGATGACCATGTCATGGTGCTGCACTATGCTAGAACGTCCTCGTCTTCTACAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

48.75

Weight (kDa)

6.2

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 350 - 399 7.9e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 414
AccB1I GGYRCC 2 cut(s) 86, 109
AccB7I CCANNNNNTGG 1 cut(s) 1285
AccIII TCCGGA 2 cut(s) 421, 461
AciI CCGC 5 cut(s) 84, 317, 387, 701, 943
AclWI GGATC 3 cut(s) 751, 926, 1043
AcoI YGGCCR 2 cut(s) 264, 388
AcsI RAATTY 1 cut(s) 597
AcyI GRCGYC 2 cut(s) 110, 411
AfiI CCNNNNNNNGG 8 cut(s) 318, 421, 614, 667, 668, 727, 953, 1285
AflIII ACRYGT 1 cut(s) 1124
AgsI TTSAA 4 cut(s) 656, 795, 866, 872
AjnI CCWGG 2 cut(s) 548, 726
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 3 cut(s) 751, 926, 1043
Aor13HI TCCGGA 2 cut(s) 421, 461
AoxI GGCC 5 cut(s) 264, 388, 749, 905, 944
ApoI RAATTY 1 cut(s) 597
ArsI GACNNNNNNTTYG 4 cut(s) 110, 142, 485, 517
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 4 cut(s) 257, 830, 905, 945
AsuC2I CCSGG 1 cut(s) 663
AvaII GGWCC 2 cut(s) 257, 830
AxyI CCTNAGG 1 cut(s) 1031
BanI GGYRCC 2 cut(s) 86, 109
BbsI GAAGAC 2 cut(s) 546, 1308
BccI CCATC 3 cut(s) 256, 360, 1037
BceAI ACGGC 3 cut(s) 123, 490, 539
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 2 cut(s) 550, 728
BclI TGATCA 2 cut(s) 169, 211
BcnI CCSGG 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 351
BfaI CTAG 2 cut(s) 242, 1302
BfmI CTRYAG 1 cut(s) 339
BfoI RGCGCY 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 550, 663, 728
Bme18I GGWCC 2 cut(s) 257, 830
BmgT120I GGNCC 4 cut(s) 257, 830, 905, 945
BmiI GGNNCC 6 cut(s) 12, 88, 111, 371, 831, 947
BmrFI CCNGG 3 cut(s) 550, 663, 728
BmrI ACTGGG 3 cut(s) 748, 836, 943
BmsI GCATC 5 cut(s) 239, 467, 845, 966, 1226
BmuI ACTGGG 3 cut(s) 748, 836, 943
BpiI GAAGAC 2 cut(s) 546, 1308
BplI GAGNNNNNCTC 2 cut(s) 697, 729
BpuMI CCSGG 1 cut(s) 663
BsaBI GATNNNNATC 2 cut(s) 348, 588
BsaHI GRCGYC 2 cut(s) 110, 411
BsaJI CCNNGG 3 cut(s) 6, 661, 938
BsaWI WCCGGW 2 cut(s) 421, 461
BsaXI ACNNNNNCTCC 2 cut(s) 1125, 1155
Bsc4I CCNNNNNNNGG 8 cut(s) 318, 421, 614, 667, 668, 727, 953, 1285
Bse1I ACTGG 4 cut(s) 402, 743, 831, 949
Bse21I CCTNAGG 1 cut(s) 1031
Bse8I GATNNNNATC 2 cut(s) 348, 588
BseAI TCCGGA 2 cut(s) 421, 461
BseBI CCWGG 2 cut(s) 550, 728
BseDI CCNNGG 3 cut(s) 6, 661, 938
BseGI GGATG 5 cut(s) 52, 458, 836, 1048, 1056
BseJI GATNNNNATC 2 cut(s) 348, 588
BseLI CCNNNNNNNGG 8 cut(s) 318, 421, 614, 667, 668, 727, 953, 1285
BseMII CTCAG 1 cut(s) 1045
BseNI ACTGG 4 cut(s) 402, 743, 831, 949
BseRI GAGGAG 5 cut(s) 832, 927, 1240, 1243, 1246
BseYI CCCAGC 1 cut(s) 902
BsgI GTGCAG 2 cut(s) 426, 1277
BshFI GGCC 5 cut(s) 266, 390, 751, 907, 946
BshNI GGYRCC 2 cut(s) 86, 109
BsiSI CCGG 4 cut(s) 113, 422, 462, 663
BslFI GGGAC 2 cut(s) 843, 1208
BslI CCNNNNNNNGG 8 cut(s) 318, 421, 614, 667, 668, 727, 953, 1285
BsmAI GTCTC 1 cut(s) 351
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 2 cut(s) 843, 1208
BsnI GGCC 5 cut(s) 266, 390, 751, 907, 946
Bsp13I TCCGGA 2 cut(s) 421, 461
Bsp143I GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 5 cut(s) 84, 317, 387, 701, 943
BspANI GGCC 5 cut(s) 266, 390, 751, 907, 946
BspCNI CTCAG 1 cut(s) 1044
BspEI TCCGGA 2 cut(s) 421, 461
BspLI GGNNCC 6 cut(s) 12, 88, 111, 371, 831, 947
BspPI GGATC 3 cut(s) 751, 926, 1043
BspT107I GGYRCC 2 cut(s) 86, 109
BsrI ACTGG 4 cut(s) 402, 743, 831, 949
BssECI CCNNGG 3 cut(s) 6, 661, 938
BssMI GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
BssNI GRCGYC 2 cut(s) 110, 411
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 550, 728
Bst4CI ACNGT 4 cut(s) 68, 290, 1002, 1057
Bst6I CTCTTC 2 cut(s) 811, 817
BstACI GRCGYC 2 cut(s) 110, 411
BstC8I GCNNGC 1 cut(s) 856
BstDEI CTNAG 4 cut(s) 913, 1031, 1091, 1190
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 5 cut(s) 52, 458, 836, 1048, 1056
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 9 cut(s) 172, 214, 241, 352, 481, 586, 759, 934, 1038
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
BstMWI GCNNNNNNNGC 5 cut(s) 404, 1205, 1208, 1211, 1214
BstNI CCWGG 2 cut(s) 550, 728
BstNSI RCATGY 2 cut(s) 1128, 1169
BstSCI CCNGG 3 cut(s) 548, 661, 726
BstSFI CTRYAG 1 cut(s) 339
BstV2I GAAGAC 2 cut(s) 546, 1308
Bsu36I CCTNAGG 1 cut(s) 1031
BsuRI GGCC 5 cut(s) 266, 390, 751, 907, 946
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 5 cut(s) 52, 458, 836, 1048, 1056
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 856
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 257, 830, 905, 945
CseI GACGC 1 cut(s) 1205
CviAII CATG 8 cut(s) 7, 568, 575, 890, 1125, 1166, 1280, 1285
DdeI CTNAG 4 cut(s) 913, 1031, 1091, 1190
DinI GGCGCC 1 cut(s) 111
DpnI GATC 9 cut(s) 171, 213, 240, 351, 480, 585, 758, 933, 1037
DpnII GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
EaeI YGGCCR 2 cut(s) 264, 388
Eam1104I CTCTTC 2 cut(s) 811, 817
EarI CTCTTC 2 cut(s) 811, 817
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 257, 830
Eco81I CCTNAGG 1 cut(s) 1031
EcoRII CCWGG 2 cut(s) 548, 726
EcoT14I CCWWGG 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 8 cut(s) 10, 571, 578, 893, 1128, 1169, 1283, 1288
FaqI GGGAC 2 cut(s) 843, 1208
FatI CATG 8 cut(s) 6, 567, 574, 889, 1124, 1165, 1279, 1284
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 2 cut(s) 169, 211
FokI GGATG 5 cut(s) 39, 445, 823, 1055, 1063
FspBI CTAG 2 cut(s) 242, 1302
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 5 cut(s) 266, 390, 751, 907, 946
HapII CCGG 4 cut(s) 113, 422, 462, 663
HgaI GACGC 1 cut(s) 1205
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 2 cut(s) 110, 411
Hin1II CATG 8 cut(s) 10, 571, 578, 893, 1128, 1169, 1283, 1288
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 7 cut(s) 145, 275, 562, 1095, 1106, 1186, 1244
HpaI GTTAAC 1 cut(s) 736
HpaII CCGG 4 cut(s) 113, 422, 462, 663
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 5 cut(s) 238, 261, 354, 923, 1111
Hpy188III TCNNGA 9 cut(s) 39, 279, 422, 462, 581, 989, 1033, 1153, 1241
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 4 cut(s) 122, 505, 511, 514
HpyAV CCTTC 3 cut(s) 24, 152, 1041
HpyCH4III ACNGT 4 cut(s) 68, 290, 1002, 1057
HpyCH4IV ACGT 4 cut(s) 411, 638, 1157, 1307
HpyCH4V TGCA 7 cut(s) 20, 407, 538, 823, 854, 1162, 1294
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 1205, 1208, 1211, 1214
HpyF3I CTNAG 4 cut(s) 913, 1031, 1091, 1190
HpySE526I ACGT 4 cut(s) 411, 638, 1157, 1307
Hsp92I GRCGYC 2 cut(s) 110, 411
Hsp92II CATG 8 cut(s) 10, 571, 578, 893, 1128, 1169, 1283, 1288
HspAI GCGC 1 cut(s) 110
KasI GGCGCC 1 cut(s) 109
Kpn2I TCCGGA 2 cut(s) 421, 461
Ksp22I TGATCA 2 cut(s) 169, 211
KspAI GTTAAC 1 cut(s) 736
Kzo9I GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
LmnI GCTCC 4 cut(s) 340, 375, 424, 1146
LweI GCATC 5 cut(s) 239, 467, 845, 966, 1226
MaeI CTAG 2 cut(s) 242, 1302
MaeII ACGT 4 cut(s) 411, 638, 1157, 1307
MaeIII GTNAC 1 cut(s) 519
MalI GATC 9 cut(s) 171, 213, 240, 351, 480, 585, 758, 933, 1037
MboI GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
MboII GAAGA 9 cut(s) 225, 281, 359, 441, 546, 798, 804, 875, 1308
MfeI CAATTG 1 cut(s) 374
MluCI AATT 7 cut(s) 91, 374, 527, 597, 610, 622, 643
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 3 cut(s) 556, 1089, 1195
MroI TCCGGA 2 cut(s) 421, 461
MseI TTAA 5 cut(s) 252, 294, 440, 735, 1083
MslI CAYNNNNRTG 2 cut(s) 891, 894
MspI CCGG 4 cut(s) 113, 422, 462, 663
MspR9I CCNGG 3 cut(s) 550, 663, 728
MunI CAATTG 1 cut(s) 374
MvaI CCWGG 2 cut(s) 550, 728
MwoI GCNNNNNNNGC 5 cut(s) 404, 1205, 1208, 1211, 1214
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 663
NcoI CCATGG 1 cut(s) 6
NdeII GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
NlaIII CATG 8 cut(s) 10, 571, 578, 893, 1128, 1169, 1283, 1288
NlaIV GGNNCC 6 cut(s) 12, 88, 111, 371, 831, 947
NmeAIII GCCGAG 3 cut(s) 729, 919, 933
NmuCI GTSAC 1 cut(s) 519
NspI RCATGY 2 cut(s) 1128, 1169
PciI ACATGT 1 cut(s) 1124
PcsI WCGNNNNNNNCGW 3 cut(s) 506, 509, 515
PfeI GAWTC 4 cut(s) 145, 275, 1106, 1244
PflFI GACNNNGTC 1 cut(s) 1280
PflMI CCANNNNNTGG 1 cut(s) 1285
PleI GAGTC 3 cut(s) 556, 1089, 1194
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 3 cut(s) 556, 1089, 1194
PscI ACATGT 1 cut(s) 1124
Psp6I CCWGG 2 cut(s) 548, 726
PspFI CCCAGC 1 cut(s) 902
PspGI CCWGG 2 cut(s) 548, 726
PspN4I GGNNCC 6 cut(s) 12, 88, 111, 371, 831, 947
PspPI GGNCC 4 cut(s) 257, 830, 905, 945
PsyI GACNNNGTC 1 cut(s) 1280
RseI CAYNNNNRTG 2 cut(s) 891, 894
SaqAI TTAA 5 cut(s) 252, 294, 440, 735, 1083
Sau3AI GATC 9 cut(s) 169, 211, 238, 349, 478, 583, 756, 931, 1035
Sau96I GGNCC 4 cut(s) 257, 830, 905, 945
SchI GAGTC 3 cut(s) 556, 1089, 1195
ScrFI CCNGG 3 cut(s) 550, 663, 728
SfaNI GCATC 5 cut(s) 239, 467, 845, 966, 1226
SfcI CTRYAG 1 cut(s) 339
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 2 cut(s) 257, 830
SmiMI CAYNNNNRTG 2 cut(s) 891, 894
Sse9I AATT 7 cut(s) 91, 374, 527, 597, 610, 622, 643
SsiI CCGC 5 cut(s) 84, 317, 387, 701, 943
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 2 cut(s) 242, 1302
StyD4I CCNGG 3 cut(s) 548, 661, 726
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 4 cut(s) 68, 290, 1002, 1057
TaiI ACGT 4 cut(s) 414, 641, 1160, 1310
TaqI TCGA 3 cut(s) 1060, 1098, 1129
TasI AATT 7 cut(s) 91, 374, 527, 597, 610, 622, 643
TauI GCSGC 3 cut(s) 390, 703, 946
TfiI GAWTC 4 cut(s) 145, 275, 1106, 1244
Tru1I TTAA 5 cut(s) 252, 294, 440, 735, 1083
Tru9I TTAA 5 cut(s) 252, 294, 440, 735, 1083
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 519
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 7 cut(s) 158, 288, 360, 438, 563, 644, 875
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 1280
Van91I CCANNNNNTGG 1 cut(s) 1285
VpaK11BI GGWCC 2 cut(s) 257, 830
XapI RAATTY 1 cut(s) 597
XceI RCATGY 2 cut(s) 1128, 1169
XspI CTAG 2 cut(s) 242, 1302
ZraI GACGTC 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.