MD13G1127900.v1.1

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006209
Physical Location & Seq
Forward (+)
9688154 .. 9697279
9126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1127900.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGACCATGGTTCCTCCTGCTGCTCATCATCAAAACATACCCAACCAACAACAACAACACAATCTCTACGGCGGTTATGGTGGTTGTGGTTCCAATTACACGGCGGAGATCAGCAATTTCGGTCTTATGAATACTAGTCAAGTTGTAGAAGAAGGTTATGAGTATAACAATTATTGCAATAATATTGTTATTGAATCTGATCATCATCATCATCATCAGCAGCAGCTAGCGGGAATTGATTCAATGGCCGAGGATGAGTCGAGAACTAACAGCGTAAACGAAGCAGGTTCGAGTTCTAAAGATGCTCCGGAAGATAACAAAACATGGCTCCAATTGACTATAGGCGGCGGCTCTAACAACCACCCACCTGCGACTACAGAACATCCAACCCTCAGAAGCTTACGAAGTAGTACTACTCCTTCCCGTCCCGGATTGGTTGAACTTGATCTTGATCTATTACCACCAGGCCATGATCAAGGTGGTTTTTCGTCATTACGGCAAGTGAGGTCCATGCCACATATGCCTCCAATCTTTCATGTTTCTCAACACGAAAATATTTTCCGTGCTTCTCCTTCGAATCCGTCTTTCAGGCCTCCGTTTAATTCGAGTAACGCTACGGCTTTATACTTCCAGCAACCGGGGCCGGGAATGAGTAGCTCCTCGTCGGGTTTATTTCCTCACCAGGACGTTAACTGGGCATTTAGGCCAGCAATCCCTCATAATAATATAGGGATGATGGCTTCTACTTCATCTTCTTCTGCTTCCTCTTTTATACAACTGGGTTCATCCTATTTTGCACGCCCGTTCCACCATCCTCTTCATGAATTTAATGTCGCCGGACCGAGCTTAGATTTCAGAGTTGTTGATCCTCCGAGAAGGCCCCAATCTGGCATTTGGTTTACGCTCCAAGCATCCCAAAATCAAGCAAAAGAACCATTCTTGCCCCAGATACCCAAAAGCTACTTAAGAATCAAGGATGGAAGAATGACAGTTCGGTTGATAAAGAAGTATCTGGTTAACAAGCTGAGATTGGATAGCGAATCAGAGGTAGAGATAACATGTAGAGGACAGGAGCTTCTACCTTTCTTGACGTTGCAGCATGTAAGAGACAACATCTGGAGTCTAAGGGACGCACTCACTTTGCTTCCAGACTCCTCAACCACTGATCATGTCATGGTGCTGAACTATGCTAGGACCACGACGATGACGAAGACGACGACTACTACAACCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

412

Amino Acids

45.67

Weight (kDa)

6.48

Isoelectric Point (pI)

65.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 328 - 377 7.5e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 376
Acc36I ACCTGC 2 cut(s) 275, 376
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 6 cut(s) 72, 104, 230, 345, 348, 1230
AclWI GGATC 1 cut(s) 860
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 824
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 3 cut(s) 637, 644, 887
AflII CTTAAG 1 cut(s) 964
AflIII ACRYGT 1 cut(s) 1058
AgsI TTSAA 3 cut(s) 194, 243, 440
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 2 cut(s) 463, 681
AluBI AGCT 7 cut(s) 226, 399, 657, 846, 960, 1024, 1075
AluI AGCT 7 cut(s) 226, 399, 657, 846, 960, 1024, 1075
Alw26I GTCTC 1 cut(s) 1101
AlwI GGATC 1 cut(s) 860
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 6 cut(s) 246, 466, 590, 641, 704, 878
ApeKI GCWGC 4 cut(s) 20, 220, 223, 1096
ApoI RAATTY 1 cut(s) 824
Asp700I GAANNNNTTC 1 cut(s) 238
AspS9I GGNCC 5 cut(s) 507, 641, 839, 879, 1194
AsuC2I CCSGG 3 cut(s) 429, 639, 645
AsuHPI GGTGA 1 cut(s) 671
AsuII TTCGAA 1 cut(s) 575
AsuNHI GCTAGC 1 cut(s) 226
AvaII GGWCC 3 cut(s) 507, 839, 1194
BarI GAAGNNNNNNTAC 4 cut(s) 397, 403, 429, 435
BbsI GAAGAC 1 cut(s) 1217
BbvI GCAGC 4 cut(s) 7, 232, 235, 1108
BccI CCATC 3 cut(s) 730, 819, 971
BceAI ACGGC 4 cut(s) 85, 117, 512, 633
BciT130I CCWGG 2 cut(s) 465, 683
BclI TGATCA 3 cut(s) 199, 472, 1165
BcnI CCSGG 3 cut(s) 429, 639, 645
BcoDI GTCTC 1 cut(s) 1101
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 3 cut(s) 135, 227, 1191
BfmI CTRYAG 2 cut(s) 339, 375
BfrI CTTAAG 1 cut(s) 964
BfuAI ACCTGC 2 cut(s) 275, 376
BisI GCNGC 6 cut(s) 21, 221, 224, 346, 349, 1097
BlsI GCNGC 6 cut(s) 22, 222, 225, 347, 350, 1098
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 5 cut(s) 429, 465, 639, 645, 683
Bme18I GGWCC 3 cut(s) 507, 839, 1194
BmgT120I GGNCC 5 cut(s) 507, 641, 839, 879, 1194
BmiI GGNNCC 5 cut(s) 12, 91, 329, 642, 881
BmrFI CCNGG 5 cut(s) 429, 465, 639, 645, 683
BmrI ACTGGG 2 cut(s) 703, 788
BmsI GCATC 2 cut(s) 292, 920
BmtI GCTAGC 1 cut(s) 230
BmuI ACTGGG 2 cut(s) 703, 788
BpiI GAAGAC 1 cut(s) 1217
BpmI CTGGAG 1 cut(s) 1138
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 3 cut(s) 429, 639, 645
BsaBI GATNNNNATC 2 cut(s) 204, 450
BsaJI CCNNGG 3 cut(s) 6, 249, 638
BsaWI WCCGGW 1 cut(s) 307
Bsc4I CCNNNNNNNGG 3 cut(s) 637, 644, 887
Bse1I ACTGG 2 cut(s) 698, 783
Bse8I GATNNNNATC 2 cut(s) 204, 450
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 2 cut(s) 465, 683
BseDI CCNNGG 3 cut(s) 6, 249, 638
BseGI GGATG 7 cut(s) 259, 382, 738, 785, 811, 911, 982
BseJI GATNNNNATC 2 cut(s) 204, 450
BseLI CCNNNNNNNGG 3 cut(s) 637, 644, 887
BseMII CTCAG 2 cut(s) 406, 1016
BseNI ACTGG 2 cut(s) 698, 783
BseRI GAGGAG 2 cut(s) 649, 1144
BseXI GCAGC 4 cut(s) 7, 232, 235, 1108
BshFI GGCC 6 cut(s) 248, 468, 592, 643, 706, 880
BsiSI CCGG 5 cut(s) 308, 429, 638, 644, 837
BslFI GGGAC 2 cut(s) 411, 1142
BslI CCNNNNNNNGG 3 cut(s) 637, 644, 887
BsmAI GTCTC 1 cut(s) 1101
BsmFI GGGAC 2 cut(s) 411, 1142
BsnI GGCC 6 cut(s) 248, 468, 592, 643, 706, 880
Bsp119I TTCGAA 1 cut(s) 575
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 72, 104, 230, 345, 348, 1230
BspANI GGCC 6 cut(s) 248, 468, 592, 643, 706, 880
BspCNI CTCAG 2 cut(s) 405, 1017
BspEI TCCGGA 1 cut(s) 307
BspHI TCATGA 1 cut(s) 820
BspLI GGNNCC 5 cut(s) 12, 91, 329, 642, 881
BspMI ACCTGC 2 cut(s) 275, 376
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 860
BspT104I TTCGAA 1 cut(s) 575
BspTI CTTAAG 1 cut(s) 964
BsrI ACTGG 2 cut(s) 698, 783
BssECI CCNNGG 3 cut(s) 6, 249, 638
BssMI GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 465, 683
Bst4CI ACNGT 1 cut(s) 991
Bst6I CTCTTC 1 cut(s) 822
BstAFI CTTAAG 1 cut(s) 964
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 228, 708, 799
BstDEI CTNAG 4 cut(s) 392, 847, 1025, 1124
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 7 cut(s) 259, 382, 738, 785, 811, 911, 982
BstKTI GATC 7 cut(s) 111, 202, 448, 454, 475, 868, 1168
BstMAI GTCTC 1 cut(s) 1101
BstMBI GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
BstMWI GCNNNNNNNGC 2 cut(s) 520, 640
BstNI CCWGG 2 cut(s) 465, 683
BstNSI RCATGY 2 cut(s) 1062, 1103
BstSCI CCNGG 5 cut(s) 427, 463, 637, 643, 681
BstSFI CTRYAG 2 cut(s) 339, 375
BstV1I GCAGC 4 cut(s) 7, 232, 235, 1108
BstV2I GAAGAC 1 cut(s) 1217
BsuRI GGCC 6 cut(s) 248, 468, 592, 643, 706, 880
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 7 cut(s) 259, 382, 738, 785, 811, 911, 982
BtsIMutI CAGTG 1 cut(s) 1161
BveI ACCTGC 2 cut(s) 275, 376
Cac8I GCNNGC 3 cut(s) 228, 708, 799
CciI TCATGA 1 cut(s) 820
Cfr13I GGNCC 5 cut(s) 507, 641, 839, 879, 1194
CpoI CGGWCCG 1 cut(s) 839
CseI GACGC 1 cut(s) 1139
Csp6I GTAC 1 cut(s) 411
CspI CGGWCCG 1 cut(s) 839
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 4 cut(s) 392, 847, 1025, 1124
DpnI GATC 7 cut(s) 110, 201, 447, 453, 474, 867, 1167
DpnII GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 6
Eco147I AGGCCT 1 cut(s) 592
Eco47I GGWCC 3 cut(s) 507, 839, 1194
EcoO109I RGGNCCY 1 cut(s) 879
EcoRII CCWGG 2 cut(s) 463, 681
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaqI GGGAC 2 cut(s) 411, 1142
FauI CCCGC 1 cut(s) 223
FauNDI CATATG 1 cut(s) 519
FbaI TGATCA 3 cut(s) 199, 472, 1165
Fnu4HI GCNGC 6 cut(s) 21, 221, 224, 346, 349, 1097
FokI GGATG 7 cut(s) 266, 369, 745, 772, 798, 898, 989
Fsp4HI GCNGC 6 cut(s) 21, 221, 224, 346, 349, 1097
FspBI CTAG 3 cut(s) 135, 227, 1191
GluI GCNGC 6 cut(s) 21, 221, 224, 346, 349, 1097
GsuI CTGGAG 1 cut(s) 1138
HaeIII GGCC 6 cut(s) 248, 468, 592, 643, 706, 880
HapII CCGG 5 cut(s) 308, 429, 638, 644, 837
HgaI GACGC 1 cut(s) 1139
HincII GTYRAC 2 cut(s) 691, 1018
HindII GTYRAC 2 cut(s) 691, 1018
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 8 cut(s) 194, 239, 257, 577, 969, 1040, 1120, 1151
HpaI GTTAAC 2 cut(s) 691, 1018
HpaII CCGG 5 cut(s) 308, 429, 638, 644, 837
HphI GGTGA 1 cut(s) 671
Hpy166II GTNNAC 4 cut(s) 277, 691, 900, 1018
Hpy188I TCNGA 5 cut(s) 199, 395, 857, 873, 1045
Hpy188III TCNNGA 7 cut(s) 261, 308, 449, 821, 1087, 1117, 1148
Hpy8I GTNNAC 4 cut(s) 277, 691, 900, 1018
Hpy99I CGWCG 3 cut(s) 667, 1204, 1219
HpyAV CCTTC 4 cut(s) 146, 429, 582, 870
HpyCH4III ACNGT 1 cut(s) 991
HpyCH4IV ACGT 2 cut(s) 687, 1091
HpyCH4V TGCA 3 cut(s) 177, 797, 1096
HpyF10VI GCNNNNNNNGC 2 cut(s) 520, 640
HpyF3I CTNAG 4 cut(s) 392, 847, 1025, 1124
HpySE526I ACGT 2 cut(s) 687, 1091
Kpn2I TCCGGA 1 cut(s) 307
Ksp22I TGATCA 3 cut(s) 199, 472, 1165
KspAI GTTAAC 2 cut(s) 691, 1018
Kzo9I GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
LmnI GCTCC 5 cut(s) 310, 333, 662, 909, 1072
Lsp1109I GCAGC 4 cut(s) 7, 232, 235, 1108
LweI GCATC 2 cut(s) 292, 920
MaeI CTAG 3 cut(s) 135, 227, 1191
MaeII ACGT 2 cut(s) 687, 1091
MaeIII GTNAC 1 cut(s) 608
MalI GATC 7 cut(s) 110, 201, 447, 453, 474, 867, 1167
MboI GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
MboII GAAGA 7 cut(s) 161, 323, 744, 747, 809, 993, 1222
MfeI CAATTG 1 cut(s) 332
MluCI AATT 7 cut(s) 94, 115, 169, 234, 332, 601, 824
MlyI GAGTC 3 cut(s) 266, 1129, 1145
MmeI TCCRAC 1 cut(s) 410
MroI TCCGGA 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 5 cut(s) 600, 690, 828, 965, 1017
MslI CAYNNNNRTG 1 cut(s) 1202
MspCI CTTAAG 1 cut(s) 964
MspI CCGG 5 cut(s) 308, 429, 638, 644, 837
MspR9I CCNGG 5 cut(s) 429, 465, 639, 645, 683
MunI CAATTG 1 cut(s) 332
MvaI CCWGG 2 cut(s) 465, 683
MwoI GCNNNNNNNGC 2 cut(s) 520, 640
NciI CCSGG 3 cut(s) 429, 639, 645
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 519
NdeII GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
NheI GCTAGC 1 cut(s) 226
NlaIV GGNNCC 5 cut(s) 12, 91, 329, 642, 881
NmeAIII GCCGAG 1 cut(s) 274
NspI RCATGY 2 cut(s) 1062, 1103
NspV TTCGAA 1 cut(s) 575
PagI TCATGA 1 cut(s) 820
PaqCI CACCTGC 1 cut(s) 376
PceI AGGCCT 1 cut(s) 592
PciI ACATGT 1 cut(s) 1058
PcsI WCGNNNNNNNCGW 2 cut(s) 1205, 1214
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 5 cut(s) 194, 239, 577, 969, 1040
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 6 cut(s) 22, 222, 225, 347, 350, 1098
PleI GAGTC 3 cut(s) 265, 1128, 1145
PpsI GAGTC 3 cut(s) 265, 1128, 1145
PscI ACATGT 1 cut(s) 1058
Psp6I CCWGG 2 cut(s) 463, 681
PspGI CCWGG 2 cut(s) 463, 681
PspN4I GGNNCC 5 cut(s) 12, 91, 329, 642, 881
PspPI GGNCC 5 cut(s) 507, 641, 839, 879, 1194
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 1202
Rsr2I CGGWCCG 1 cut(s) 839
RsrII CGGWCCG 1 cut(s) 839
SaqAI TTAA 5 cut(s) 600, 690, 828, 965, 1017
SatI GCNGC 6 cut(s) 21, 221, 224, 346, 349, 1097
Sau3AI GATC 7 cut(s) 108, 199, 445, 451, 472, 865, 1165
Sau96I GGNCC 5 cut(s) 507, 641, 839, 879, 1194
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 3 cut(s) 266, 1129, 1145
ScrFI CCNGG 5 cut(s) 429, 465, 639, 645, 683
SfaNI GCATC 2 cut(s) 292, 920
SfcI CTRYAG 2 cut(s) 339, 375
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 3 cut(s) 507, 839, 1194
SmiMI CAYNNNNRTG 1 cut(s) 1202
SmlI CTYRAG 1 cut(s) 964
SmoI CTYRAG 1 cut(s) 964
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 7 cut(s) 94, 115, 169, 234, 332, 601, 824
SseBI AGGCCT 1 cut(s) 592
SsiI CCGC 6 cut(s) 72, 104, 230, 345, 348, 1230
SspI AATATT 2 cut(s) 184, 556
SspMI CTAG 3 cut(s) 135, 227, 1191
StuI AGGCCT 1 cut(s) 592
StyD4I CCNGG 5 cut(s) 427, 463, 637, 643, 681
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 991
TaiI ACGT 2 cut(s) 690, 1094
TaqI TCGA 4 cut(s) 260, 290, 575, 605
TaqII GACCGA 2 cut(s) 110, 856
TasI AATT 7 cut(s) 94, 115, 169, 234, 332, 601, 824
TatI WGTACW 1 cut(s) 410
TauI GCSGC 2 cut(s) 348, 351
TfiI GAWTC 5 cut(s) 194, 239, 577, 969, 1040
Tru1I TTAA 5 cut(s) 600, 690, 828, 965, 1017
Tru9I TTAA 5 cut(s) 600, 690, 828, 965, 1017
TscAI CASTG 1 cut(s) 1168
TseI GCWGC 4 cut(s) 20, 220, 223, 1096
TspDTI ATGAA 6 cut(s) 143, 524, 738, 774, 809, 837
TspGWI ACGGA 3 cut(s) 551, 570, 585
TspRI CASTG 1 cut(s) 1168
Vha464I CTTAAG 1 cut(s) 964
VpaK11BI GGWCC 3 cut(s) 507, 839, 1194
XapI RAATTY 1 cut(s) 824
XceI RCATGY 2 cut(s) 1062, 1103
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 238
XspI CTAG 3 cut(s) 135, 227, 1191
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.