FvH4_4g22950

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006209
Physical Location & Seq
Reverse (-)
25585036 .. 25592819
7784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22950.t1

Sequence Viewer

Length: 1326 bp
ATGACCATGGTTCCTTCTGCACATCATCAAAACTTATCCAGACAACAACAACAACACCCTCATCTCTACGGTGGTTATGGTGGCTGCGGTGCCAATTATTACAACAATACGGCGCCGGACGACGAGAGCTTTGGTGGTCTTCTTATGAATCAAGTAGAGGAAGGTGGTTATGATCAGTATAGTTATAATAATACTATTACTGATCAATCTTACTTATTAGCATCTTCCGATCTAGTAGTGGTTAATGGTCCAATGGCAGAAGATGAATCAAGAACTAACAGTCTTAACAACGAAGCTGAAGCCGAAGCGGGATCAAGTTCTAAAGCTCACGTAGATGAAGATCAGAGAGACGAAAGATGGCTCCAATTGAGTATAGGCGGCCACACCGCTACGGGTGCAGACGTCCAAGCTCCGGAAAGAAGGAGCTCGTTAAGGGGTTCATCATCGGCATCGGGATTGATTGAGCTTGATCTGTTATCAGCAAATATTTCGTCGCCGTCGTCTTCATCATCACGGCAGTTTCAGTTGCAACAAGGTGCTGCCAGGTCTTCTATGACTCCCATGTTTCATGTTCCCGATCATCATCATCATCATCAAATTCGTGTTCCTAATTTCGGCATGAGCACTACGTCTAATTTATATTTTCAACACCCGGGTTTCGGAATGGGAATGGGAATGAGTAGTTCTATGCCGCCGAGCTTTCATCAACACCAGCACCAGGAGGTTAACTGGGGATTTAGGCCATTGATCCCTCATCATCATAATAATCATATAGGGAATAGTAGTGCTTCTACTTCTACCTCTTCGTCTTCTTTTATTCAACTGGGACCATCCTCCTCCACCTCCTATTTTGCACGCCCATTTCAACTTCAACATCATCATCTTCATCATGGTGGTGGTGCTGCCCCGAGCTTAGATTTCAGAGTTGTTGATCCTCCGAGGCGTCCCCAGTCCGGCATCTGGTTTATGCTACAAGCCTCACAAAATCAAGAAAAAGAGCCGTTCTTGCCCCAGATACCTAAAAGCTACCTCAGAATCAAAGATGGAAGGATGACAGTTCGATTGATAAAGAAGTATCTGGTTAACAAGCTAAGACTTGACAGCGAATCAGAGGTAGAGATAACATGTAGAGGACAGCAGCTTCTACCTTTCTTGACGTTGCAGCATGTAAGAGATAATATATGGAGTCCAAGGGACGCAGCAGCATCACTCACTTTGCTTCCACTTCCAGACTTGTCCTCCTGCACCTCCACATCAACAACCACAGATGACCATGTCATGGTGCTGCACTATGCTAGGACGTCTTCGTCTTCTACAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

442

Amino Acids

48.71

Weight (kDa)

6.32

Isoelectric Point (pI)

62.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 350 - 399 5.8e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
AasI GACNNNNNNGTC 1 cut(s) 1306
AatII GACGTC 2 cut(s) 405, 1304
AccB1I GGYRCC 2 cut(s) 89, 112
AccB7I CCANNNNNTGG 1 cut(s) 1279
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 5 cut(s) 87, 308, 378, 387, 692
AclWI GGATC 3 cut(s) 319, 742, 926
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 1 cut(s) 597
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 4 cut(s) 113, 402, 943, 1301
AfiI CCNNNNNNNGG 7 cut(s) 412, 614, 659, 718, 953, 960, 1279
AflIII ACRYGT 1 cut(s) 1124
AgsI TTSAA 4 cut(s) 647, 821, 866, 872
AjnI CCWGG 2 cut(s) 542, 717
Alw21I GWGCWC 2 cut(s) 428, 626
Alw26I GTCTC 1 cut(s) 342
AlwI GGATC 3 cut(s) 319, 742, 926
Ama87I CYCGRG 2 cut(s) 652, 907
Aor13HI TCCGGA 1 cut(s) 412
AoxI GGCC 2 cut(s) 379, 740
ApeKI GCWGC 8 cut(s) 84, 539, 902, 1138, 1162, 1199, 1202, 1285
ApoI RAATTY 1 cut(s) 597
ArsI GACNNNNNNTTYG 4 cut(s) 113, 145, 476, 508
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 2 cut(s) 248, 827
AsuC2I CCSGG 2 cut(s) 653, 654
AvaI CYCGRG 2 cut(s) 652, 907
AvaII GGWCC 2 cut(s) 248, 827
BanI GGYRCC 2 cut(s) 89, 112
BanII GRGCYC 1 cut(s) 428
BbsI GAAGAC 6 cut(s) 131, 495, 540, 801, 1296, 1302
Bbv12I GWGCWC 2 cut(s) 428, 626
BbvI GCAGC 8 cut(s) 71, 526, 889, 1150, 1174, 1211, 1214, 1272
BccI CCATC 3 cut(s) 351, 838, 1037
BceAI ACGGC 4 cut(s) 126, 481, 530, 985
BcgI CGANNNNNNTGC 2 cut(s) 471, 505
BciT130I CCWGG 2 cut(s) 544, 719
BclI TGATCA 2 cut(s) 172, 202
BcnI CCSGG 2 cut(s) 653, 654
BcoDI GTCTC 1 cut(s) 342
BfaI CTAG 2 cut(s) 233, 1296
BfoI RGCGCY 1 cut(s) 116
Bme1390I CCNGG 4 cut(s) 544, 653, 654, 719
Bme18I GGWCC 2 cut(s) 248, 827
BmeT110I CYCGRG 2 cut(s) 652, 907
BmgT120I GGNCC 2 cut(s) 248, 827
BmiI GGNNCC 5 cut(s) 12, 91, 114, 362, 828
BmrFI CCNGG 4 cut(s) 544, 653, 654, 719
BmrI ACTGGG 3 cut(s) 739, 833, 943
BmsI GCATC 4 cut(s) 230, 458, 966, 1214
BmuI ACTGGG 3 cut(s) 739, 833, 943
BpiI GAAGAC 6 cut(s) 131, 495, 540, 801, 1296, 1302
BpuMI CCSGG 2 cut(s) 653, 654
BsaAI YACGTR 1 cut(s) 331
BsaBI GATNNNNATC 2 cut(s) 339, 582
BsaHI GRCGYC 4 cut(s) 113, 402, 943, 1301
BsaJI CCNNGG 4 cut(s) 6, 652, 938, 1190
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 1223, 1253
Bsc4I CCNNNNNNNGG 7 cut(s) 412, 614, 659, 718, 953, 960, 1279
Bse1I ACTGG 3 cut(s) 734, 828, 949
Bse8I GATNNNNATC 2 cut(s) 339, 582
BseAI TCCGGA 1 cut(s) 412
BseBI CCWGG 2 cut(s) 544, 719
BseDI CCNNGG 4 cut(s) 6, 652, 938, 1190
BseGI GGATG 2 cut(s) 830, 1056
BseJI GATNNNNATC 2 cut(s) 339, 582
BseLI CCNNNNNNNGG 7 cut(s) 412, 614, 659, 718, 953, 960, 1279
BseMII CTCAG 1 cut(s) 1045
BseNI ACTGG 3 cut(s) 734, 828, 949
BseRI GAGGAG 1 cut(s) 826
BseXI GCAGC 8 cut(s) 71, 526, 889, 1150, 1174, 1211, 1214, 1272
BsgI GTGCAG 3 cut(s) 417, 1228, 1271
BshFI GGCC 2 cut(s) 381, 742
BshNI GGYRCC 2 cut(s) 89, 112
BsiHKAI GWGCWC 2 cut(s) 428, 626
BsiHKCI CYCGRG 2 cut(s) 652, 907
BsiSI CCGG 4 cut(s) 116, 413, 653, 954
BslFI GGGAC 3 cut(s) 840, 930, 1208
BslI CCNNNNNNNGG 7 cut(s) 412, 614, 659, 718, 953, 960, 1279
BsmAI GTCTC 1 cut(s) 342
BsmBI CGTCTC 1 cut(s) 342
BsmFI GGGAC 3 cut(s) 840, 930, 1208
BsnI GGCC 2 cut(s) 381, 742
BsoBI CYCGRG 2 cut(s) 652, 907
Bsp1286I GDGCHC 2 cut(s) 428, 626
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 5 cut(s) 87, 308, 378, 387, 692
BspANI GGCC 2 cut(s) 381, 742
BspCNI CTCAG 1 cut(s) 1044
BspEI TCCGGA 1 cut(s) 412
BspLI GGNNCC 5 cut(s) 12, 91, 114, 362, 828
BspPI GGATC 3 cut(s) 319, 742, 926
BspT107I GGYRCC 2 cut(s) 89, 112
BsrI ACTGG 3 cut(s) 734, 828, 949
BssECI CCNNGG 4 cut(s) 6, 652, 938, 1190
BssMI GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
BssNI GRCGYC 4 cut(s) 113, 402, 943, 1301
BssT1I CCWWGG 2 cut(s) 6, 1190
Bst2UI CCWGG 2 cut(s) 544, 719
Bst4CI ACNGT 3 cut(s) 71, 281, 1057
Bst6I CTCTTC 1 cut(s) 808
BstACI GRCGYC 4 cut(s) 113, 402, 943, 1301
BstBAI YACGTR 1 cut(s) 331
BstC8I GCNNGC 1 cut(s) 856
BstDEI CTNAG 3 cut(s) 913, 1031, 1091
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 830, 1056
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 9 cut(s) 175, 205, 232, 314, 343, 472, 580, 750, 934
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
BstMWI GCNNNNNNNGC 2 cut(s) 395, 1006
BstNI CCWGG 2 cut(s) 544, 719
BstNSI RCATGY 2 cut(s) 1128, 1169
BstSCI CCNGG 4 cut(s) 542, 651, 652, 717
BstV1I GCAGC 8 cut(s) 71, 526, 889, 1150, 1174, 1211, 1214, 1272
BstV2I GAAGAC 6 cut(s) 131, 495, 540, 801, 1296, 1302
BsuRI GGCC 2 cut(s) 381, 742
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 2 cut(s) 830, 1056
Cac8I GCNNGC 1 cut(s) 856
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 248, 827
Cfr9I CCCGGG 1 cut(s) 652
CseI GACGC 2 cut(s) 932, 1205
CviAII CATG 9 cut(s) 7, 562, 569, 619, 890, 1125, 1166, 1274, 1279
DdeI CTNAG 3 cut(s) 913, 1031, 1091
DinI GGCGCC 1 cut(s) 114
DpnI GATC 9 cut(s) 174, 204, 231, 313, 342, 471, 579, 749, 933
DpnII GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
DrdI GACNNNNNNGTC 1 cut(s) 1306
DseDI GACNNNNNNGTC 1 cut(s) 1306
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 808
EarI CTCTTC 1 cut(s) 808
Ecl136II GAGCTC 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 6, 1190
Eco24I GRGCYC 1 cut(s) 428
Eco47I GGWCC 2 cut(s) 248, 827
Eco53kI GAGCTC 1 cut(s) 426
Eco57I CTGAAG 1 cut(s) 318
Eco88I CYCGRG 2 cut(s) 652, 907
EcoICRI GAGCTC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 542, 717
EcoT14I CCWWGG 2 cut(s) 6, 1190
EcoT38I GRGCYC 1 cut(s) 428
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
ErhI CCWWGG 2 cut(s) 6, 1190
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 9 cut(s) 10, 565, 572, 622, 893, 1128, 1169, 1277, 1282
FaqI GGGAC 3 cut(s) 840, 930, 1208
FatI CATG 9 cut(s) 6, 561, 568, 618, 889, 1124, 1165, 1273, 1278
FauI CCCGC 1 cut(s) 301
FbaI TGATCA 2 cut(s) 172, 202
FokI GGATG 2 cut(s) 817, 1063
FriOI GRGCYC 1 cut(s) 428
FspBI CTAG 2 cut(s) 233, 1296
GlaI GCGC 1 cut(s) 114
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 2 cut(s) 381, 742
HapII CCGG 4 cut(s) 116, 413, 653, 954
HgaI GACGC 2 cut(s) 932, 1205
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 4 cut(s) 113, 402, 943, 1301
Hin1II CATG 9 cut(s) 10, 565, 572, 622, 893, 1128, 1169, 1277, 1282
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 2 cut(s) 727, 1084
HindII GTYRAC 2 cut(s) 727, 1084
HinfI GANTC 6 cut(s) 148, 266, 556, 1035, 1106, 1186
HpaI GTTAAC 2 cut(s) 727, 1084
HpaII CCGG 4 cut(s) 116, 413, 653, 954
Hpy166II GTNNAC 2 cut(s) 727, 1084
Hpy188I TCNGA 7 cut(s) 229, 345, 662, 923, 939, 1034, 1111
Hpy188III TCNNGA 8 cut(s) 39, 270, 413, 453, 575, 989, 1153, 1229
Hpy8I GTNNAC 2 cut(s) 727, 1084
Hpy99I CGWCG 3 cut(s) 125, 496, 502
HpyAV CCTTC 4 cut(s) 24, 155, 414, 1041
HpyCH4III ACNGT 3 cut(s) 71, 281, 1057
HpyCH4IV ACGT 5 cut(s) 330, 402, 629, 1157, 1301
HpyCH4V TGCA 7 cut(s) 20, 398, 529, 854, 1162, 1245, 1288
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 1006
HpyF3I CTNAG 3 cut(s) 913, 1031, 1091
HpySE526I ACGT 5 cut(s) 330, 402, 629, 1157, 1301
Hsp92I GRCGYC 4 cut(s) 113, 402, 943, 1301
Hsp92II CATG 9 cut(s) 10, 565, 572, 622, 893, 1128, 1169, 1277, 1282
HspAI GCGC 1 cut(s) 113
KasI GGCGCC 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 412
Ksp22I TGATCA 2 cut(s) 172, 202
KspAI GTTAAC 2 cut(s) 727, 1084
Kzo9I GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
LmnI GCTCC 3 cut(s) 366, 415, 423
Lsp1109I GCAGC 8 cut(s) 71, 526, 889, 1150, 1174, 1211, 1214, 1272
LweI GCATC 4 cut(s) 230, 458, 966, 1214
MaeI CTAG 2 cut(s) 233, 1296
MaeII ACGT 5 cut(s) 330, 402, 629, 1157, 1301
MalI GATC 9 cut(s) 174, 204, 231, 313, 342, 471, 579, 749, 933
MboI GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
MfeI CAATTG 1 cut(s) 365
MhlI GDGCHC 2 cut(s) 428, 626
MluCI AATT 5 cut(s) 94, 365, 597, 610, 634
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 2 cut(s) 550, 1195
MroI TCCGGA 1 cut(s) 412
MseI TTAA 5 cut(s) 243, 285, 431, 726, 1083
MslI CAYNNNNRTG 3 cut(s) 333, 891, 894
MspI CCGG 4 cut(s) 116, 413, 653, 954
MspR9I CCNGG 4 cut(s) 544, 653, 654, 719
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 2 cut(s) 544, 719
MwoI GCNNNNNNNGC 2 cut(s) 395, 1006
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 2 cut(s) 653, 654
NcoI CCATGG 1 cut(s) 6
NdeII GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
NlaIII CATG 9 cut(s) 10, 565, 572, 622, 893, 1128, 1169, 1277, 1282
NlaIV GGNNCC 5 cut(s) 12, 91, 114, 362, 828
NmeAIII GCCGAG 1 cut(s) 720
NspI RCATGY 2 cut(s) 1128, 1169
PciI ACATGT 1 cut(s) 1124
PcsI WCGNNNNNNNCGW 1 cut(s) 497
PfeI GAWTC 4 cut(s) 148, 266, 1035, 1106
PflFI GACNNNGTC 1 cut(s) 1274
PflMI CCANNNNNTGG 1 cut(s) 1279
PleI GAGTC 2 cut(s) 550, 1194
PluTI GGCGCC 1 cut(s) 116
PpsI GAGTC 2 cut(s) 550, 1194
Ppu21I YACGTR 1 cut(s) 331
PscI ACATGT 1 cut(s) 1124
PsiI TTATAA 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 542, 717
PspGI CCWGG 2 cut(s) 542, 717
PspN4I GGNNCC 5 cut(s) 12, 91, 114, 362, 828
PspPI GGNCC 2 cut(s) 248, 827
PsyI GACNNNGTC 1 cut(s) 1274
RseI CAYNNNNRTG 3 cut(s) 333, 891, 894
SacI GAGCTC 1 cut(s) 428
SaqAI TTAA 5 cut(s) 243, 285, 431, 726, 1083
Sau3AI GATC 9 cut(s) 172, 202, 229, 311, 340, 469, 577, 747, 931
Sau96I GGNCC 2 cut(s) 248, 827
SchI GAGTC 2 cut(s) 550, 1195
ScrFI CCNGG 4 cut(s) 544, 653, 654, 719
SduI GDGCHC 2 cut(s) 428, 626
SfaNI GCATC 4 cut(s) 230, 458, 966, 1214
SfoI GGCGCC 1 cut(s) 114
SinI GGWCC 2 cut(s) 248, 827
SmaI CCCGGG 1 cut(s) 654
SmiMI CAYNNNNRTG 3 cut(s) 333, 891, 894
Sse9I AATT 5 cut(s) 94, 365, 597, 610, 634
SsiI CCGC 5 cut(s) 87, 308, 378, 387, 692
SspDI GGCGCC 1 cut(s) 112
SspI AATATT 1 cut(s) 487
SspMI CTAG 2 cut(s) 233, 1296
SstI GAGCTC 1 cut(s) 428
StyD4I CCNGG 4 cut(s) 542, 651, 652, 717
StyI CCWWGG 2 cut(s) 6, 1190
TaaI ACNGT 3 cut(s) 71, 281, 1057
TaiI ACGT 5 cut(s) 333, 405, 632, 1160, 1304
TaqI TCGA 1 cut(s) 1060
TasI AATT 5 cut(s) 94, 365, 597, 610, 634
TauI GCSGC 2 cut(s) 381, 694
TfiI GAWTC 4 cut(s) 148, 266, 1035, 1106
Tru1I TTAA 5 cut(s) 243, 285, 431, 726, 1083
Tru9I TTAA 5 cut(s) 243, 285, 431, 726, 1083
TseI GCWGC 8 cut(s) 84, 539, 902, 1138, 1162, 1199, 1202, 1285
TspDTI ATGAA 8 cut(s) 161, 279, 351, 429, 495, 557, 692, 875
TspMI CCCGGG 1 cut(s) 652
Tth111I GACNNNGTC 1 cut(s) 1274
Van91I CCANNNNNTGG 1 cut(s) 1279
VpaK11BI GGWCC 2 cut(s) 248, 827
XapI RAATTY 1 cut(s) 597
XceI RCATGY 2 cut(s) 1128, 1169
XmaI CCCGGG 1 cut(s) 652
XspI CTAG 2 cut(s) 233, 1296
ZraI GACGTC 2 cut(s) 403, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.