RchiOBHm_Chr4g0429231

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006209
Physical Location & Seq
Reverse (-)
54031225 .. 54039560
8336 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39806

Sequence Viewer

Length: 1311 bp
ATGACCATGGTTCCTTCTGCACATCATCAAAACTTATCCAGACAACAACAACATCCTCATCTCTACGGTGGTTATGGTGGCTGCGGTGCCAATTATTACAACAATACGGCGCCGGACGACGAGAGCTTTGGTGGTCATCTTATGAATCAAGTAGAGGAAGGTGGTTATGATCAGTATAGTTATTGTAATAACAATAATAATACTATTACTGATCAATCTTACTTACTAGCATCTTCCGATCTAGTAGTGGTTAATGGTCCGATGGCCGAAGATGAATCAAGAACTAACAGTCTTAACAACGAAGCCGAAGCGGGGTCAAGTTCTAAAGCTCCTGTAGATGAAGATCAGAGAGACGAGAGATGGCTCCAATTGAGTATAGGCGGCCACACCGCTTCCAGTGCAGACGTCCAAGCTCCGGAAAGAAGAAGCTCGTTAAGGGGTTCATCATCGGCATCCGGATTGATTGAGCTTGATCTGTTATCAGCAAATATATCGTCGCCGTCGTCGTCATCGTCACGGCAATTTCAGTTGCAACAAGCAGCCAGGTCTTCCACGACTCCCATGTTTCATGTTCCCGATCATCATCATCAAATCCGTGTTCCTAATTTCGGCACTAATTTGATGAACACTACGTCTAATTTATATTTTCAACACCCCGGAATGGGAATGGGGATGGGAATGAGTAGTTCTTTGCCGCCGAGCTTTCCTCAACACCAGCACCAGGAGGTTAACTGGGGATTTAGGCCATTGATCCCTCATCATAATAATCATATAGGGAATAGTGCTTCAACTTCTACCTCTTCCTCTTCTTTTATGCAACTGGGACCAGCATCCTCCTCCTATTTTGCACGCCCATTTCAACTTCAACATCATCATCTTCATCATGGTGGTGGTGCTGGGCCGAGCTTAGATTTCAGAGTTGTTGATCCTCCTCGGCGGCCCCAGTCTGGCATCTGGTTTATGCTACAAGCCTCACAAAATCAAAAAGAACCGTTCTTGCCCCAGATACCTAAAAGCTACCTCAGGATCAAGGATGGAAGGATGACAGTTCGATTGATAAAGAAGTATCTGGTTAATAAGCTAAGACTCGACAGCGAATCAGAGGTAGAGATAACATGTAGAGGACAGCAGCTCCTACCTTTCTTGACGTTGCAGCATGTAAGAGATAATATATGGAGTCTAAGGGACGCAGCAGCAGCATCACTCACTTTGCTTCCACTTCCAGACTCCTCCTCCTCCACATCAACCACAGATGACCATGTCATGGTGCTGCACTATGCTAGGACGTCCTCGTCTTCTACAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

436

Amino Acids

48.04

Weight (kDa)

6.37

Isoelectric Point (pI)

61.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 347 - 396 7.4e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1291
AatII GACGTC 2 cut(s) 408, 1289
AccB1I GGYRCC 2 cut(s) 86, 109
AccB7I CCANNNNNTGG 1 cut(s) 1264
AccIII TCCGGA 2 cut(s) 415, 455
AciI CCGC 6 cut(s) 84, 311, 381, 390, 695, 937
AclWI GGATC 3 cut(s) 745, 920, 1034
AcoI YGGCCR 2 cut(s) 264, 382
AcyI GRCGYC 3 cut(s) 110, 405, 1286
AfiI CCNNNNNNNGG 8 cut(s) 312, 415, 608, 661, 662, 721, 947, 1264
AflIII ACRYGT 1 cut(s) 1115
AgsI TTSAA 4 cut(s) 650, 789, 860, 866
AjnI CCWGG 2 cut(s) 542, 720
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 3 cut(s) 745, 920, 1034
Aor13HI TCCGGA 2 cut(s) 415, 455
AoxI GGCC 5 cut(s) 264, 382, 743, 899, 938
ApeKI GCWGC 8 cut(s) 81, 539, 1129, 1153, 1190, 1193, 1196, 1270
ArsI GACNNNNNNTTYG 4 cut(s) 110, 142, 479, 511
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 4 cut(s) 257, 824, 899, 939
AsuC2I CCSGG 1 cut(s) 657
AvaII GGWCC 2 cut(s) 257, 824
AxyI CCTNAGG 1 cut(s) 1022
BanI GGYRCC 2 cut(s) 86, 109
BbsI GAAGAC 2 cut(s) 540, 1287
BbvI GCAGC 8 cut(s) 68, 551, 1141, 1165, 1202, 1205, 1208, 1257
BccI CCATC 4 cut(s) 256, 354, 667, 1028
BceAI ACGGC 3 cut(s) 123, 484, 533
BcgI CGANNNNNNTGC 2 cut(s) 474, 508
BciT130I CCWGG 2 cut(s) 544, 722
BclI TGATCA 2 cut(s) 169, 211
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 3 cut(s) 227, 242, 1281
BfmI CTRYAG 1 cut(s) 333
BfoI RGCGCY 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 544, 657, 722
Bme18I GGWCC 2 cut(s) 257, 824
BmgT120I GGNCC 4 cut(s) 257, 824, 899, 939
BmiI GGNNCC 6 cut(s) 12, 88, 111, 365, 825, 941
BmrFI CCNGG 3 cut(s) 544, 657, 722
BmrI ACTGGG 3 cut(s) 742, 830, 937
BmsI GCATC 5 cut(s) 239, 461, 839, 960, 1208
BmuI ACTGGG 3 cut(s) 742, 830, 937
BpiI GAAGAC 2 cut(s) 540, 1287
BplI GAGNNNNNCTC 2 cut(s) 691, 723
BpuMI CCSGG 1 cut(s) 657
BsaBI GATNNNNATC 2 cut(s) 342, 582
BsaHI GRCGYC 3 cut(s) 110, 405, 1286
BsaJI CCNNGG 3 cut(s) 6, 655, 932
BsaWI WCCGGW 2 cut(s) 415, 455
BsaXI ACNNNNNCTCC 4 cut(s) 1116, 1146, 1217, 1247
Bsc4I CCNNNNNNNGG 8 cut(s) 312, 415, 608, 661, 662, 721, 947, 1264
Bse1I ACTGG 4 cut(s) 396, 737, 825, 943
Bse21I CCTNAGG 1 cut(s) 1022
Bse8I GATNNNNATC 2 cut(s) 342, 582
BseAI TCCGGA 2 cut(s) 415, 455
BseBI CCWGG 2 cut(s) 544, 722
BseDI CCNNGG 3 cut(s) 6, 655, 932
BseGI GGATG 6 cut(s) 52, 452, 678, 830, 1039, 1047
BseJI GATNNNNATC 2 cut(s) 342, 582
BseLI CCNNNNNNNGG 8 cut(s) 312, 415, 608, 661, 662, 721, 947, 1264
BseMII CTCAG 1 cut(s) 1036
BseNI ACTGG 4 cut(s) 396, 737, 825, 943
BseRI GAGGAG 5 cut(s) 826, 921, 1219, 1222, 1225
BseXI GCAGC 8 cut(s) 68, 551, 1141, 1165, 1202, 1205, 1208, 1257
BseYI CCCAGC 1 cut(s) 896
BsgI GTGCAG 2 cut(s) 420, 1256
BshFI GGCC 5 cut(s) 266, 384, 745, 901, 940
BshNI GGYRCC 2 cut(s) 86, 109
BsiSI CCGG 4 cut(s) 113, 416, 456, 657
BslFI GGGAC 2 cut(s) 837, 1199
BslI CCNNNNNNNGG 8 cut(s) 312, 415, 608, 661, 662, 721, 947, 1264
BsmAI GTCTC 1 cut(s) 345
BsmBI CGTCTC 1 cut(s) 345
BsmFI GGGAC 2 cut(s) 837, 1199
BsnI GGCC 5 cut(s) 266, 384, 745, 901, 940
Bsp13I TCCGGA 2 cut(s) 415, 455
Bsp143I GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 84, 311, 381, 390, 695, 937
BspANI GGCC 5 cut(s) 266, 384, 745, 901, 940
BspCNI CTCAG 1 cut(s) 1035
BspEI TCCGGA 2 cut(s) 415, 455
BspLI GGNNCC 6 cut(s) 12, 88, 111, 365, 825, 941
BspPI GGATC 3 cut(s) 745, 920, 1034
BspT107I GGYRCC 2 cut(s) 86, 109
BsrI ACTGG 4 cut(s) 396, 737, 825, 943
BssECI CCNNGG 3 cut(s) 6, 655, 932
BssMI GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
BssNI GRCGYC 3 cut(s) 110, 405, 1286
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 544, 722
Bst4CI ACNGT 4 cut(s) 68, 290, 993, 1048
Bst6I CTCTTC 2 cut(s) 805, 811
BstACI GRCGYC 3 cut(s) 110, 405, 1286
BstC8I GCNNGC 1 cut(s) 850
BstDEI CTNAG 4 cut(s) 907, 1022, 1082, 1181
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 6 cut(s) 52, 452, 678, 830, 1039, 1047
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 9 cut(s) 172, 214, 241, 346, 475, 580, 753, 928, 1029
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
BstMWI GCNNNNNNNGC 2 cut(s) 398, 1196
BstNI CCWGG 2 cut(s) 544, 722
BstNSI RCATGY 2 cut(s) 1119, 1160
BstSCI CCNGG 3 cut(s) 542, 655, 720
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 8 cut(s) 68, 551, 1141, 1165, 1202, 1205, 1208, 1257
BstV2I GAAGAC 2 cut(s) 540, 1287
Bsu36I CCTNAGG 1 cut(s) 1022
BsuRI GGCC 5 cut(s) 266, 384, 745, 901, 940
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 6 cut(s) 52, 452, 678, 830, 1039, 1047
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 850
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 257, 824, 899, 939
CseI GACGC 1 cut(s) 1196
CviAII CATG 8 cut(s) 7, 562, 569, 884, 1116, 1157, 1259, 1264
DdeI CTNAG 4 cut(s) 907, 1022, 1082, 1181
DinI GGCGCC 1 cut(s) 111
DpnI GATC 9 cut(s) 171, 213, 240, 345, 474, 579, 752, 927, 1028
DpnII GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
DrdI GACNNNNNNGTC 1 cut(s) 1291
DseDI GACNNNNNNGTC 1 cut(s) 1291
EaeI YGGCCR 2 cut(s) 264, 382
Eam1104I CTCTTC 2 cut(s) 805, 811
EarI CTCTTC 2 cut(s) 805, 811
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 257, 824
Eco81I CCTNAGG 1 cut(s) 1022
EcoRII CCWGG 2 cut(s) 542, 720
EcoT14I CCWWGG 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 345
FaeI CATG 8 cut(s) 10, 565, 572, 887, 1119, 1160, 1262, 1267
FaqI GGGAC 2 cut(s) 837, 1199
FatI CATG 8 cut(s) 6, 561, 568, 883, 1115, 1156, 1258, 1263
FauI CCCGC 1 cut(s) 304
FbaI TGATCA 2 cut(s) 169, 211
FokI GGATG 6 cut(s) 39, 439, 685, 817, 1046, 1054
FspBI CTAG 3 cut(s) 227, 242, 1281
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 900
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 5 cut(s) 266, 384, 745, 901, 940
HapII CCGG 4 cut(s) 113, 416, 456, 657
HgaI GACGC 1 cut(s) 1196
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 3 cut(s) 110, 405, 1286
Hin1II CATG 8 cut(s) 10, 565, 572, 887, 1119, 1160, 1262, 1267
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HinfI GANTC 7 cut(s) 145, 275, 556, 1086, 1097, 1177, 1226
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 4 cut(s) 113, 416, 456, 657
Hpy166II GTNNAC 1 cut(s) 730
Hpy188I TCNGA 5 cut(s) 238, 261, 348, 917, 1102
Hpy188III TCNNGA 8 cut(s) 39, 279, 416, 456, 575, 1024, 1144, 1223
Hpy8I GTNNAC 1 cut(s) 730
Hpy99I CGWCG 4 cut(s) 122, 499, 505, 508
HpyAV CCTTC 3 cut(s) 24, 152, 1032
HpyCH4III ACNGT 4 cut(s) 68, 290, 993, 1048
HpyCH4IV ACGT 4 cut(s) 405, 632, 1148, 1286
HpyCH4V TGCA 7 cut(s) 20, 401, 532, 817, 848, 1153, 1273
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 1196
HpyF3I CTNAG 4 cut(s) 907, 1022, 1082, 1181
HpySE526I ACGT 4 cut(s) 405, 632, 1148, 1286
Hsp92I GRCGYC 3 cut(s) 110, 405, 1286
Hsp92II CATG 8 cut(s) 10, 565, 572, 887, 1119, 1160, 1262, 1267
HspAI GCGC 1 cut(s) 110
KasI GGCGCC 1 cut(s) 109
Kpn2I TCCGGA 2 cut(s) 415, 455
Ksp22I TGATCA 2 cut(s) 169, 211
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
LmnI GCTCC 4 cut(s) 334, 369, 418, 1137
Lsp1109I GCAGC 8 cut(s) 68, 551, 1141, 1165, 1202, 1205, 1208, 1257
LweI GCATC 5 cut(s) 239, 461, 839, 960, 1208
MaeI CTAG 3 cut(s) 227, 242, 1281
MaeII ACGT 4 cut(s) 405, 632, 1148, 1286
MaeIII GTNAC 1 cut(s) 513
MalI GATC 9 cut(s) 171, 213, 240, 345, 474, 579, 752, 927, 1028
MboI GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
MboII GAAGA 9 cut(s) 225, 281, 353, 435, 540, 792, 798, 869, 1287
MfeI CAATTG 1 cut(s) 368
MluCI AATT 6 cut(s) 91, 368, 521, 604, 616, 637
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 4 cut(s) 550, 1080, 1186, 1220
MroI TCCGGA 2 cut(s) 415, 455
MseI TTAA 5 cut(s) 252, 294, 434, 729, 1074
MslI CAYNNNNRTG 2 cut(s) 885, 888
MspI CCGG 4 cut(s) 113, 416, 456, 657
MspR9I CCNGG 3 cut(s) 544, 657, 722
MunI CAATTG 1 cut(s) 368
MvaI CCWGG 2 cut(s) 544, 722
MwoI GCNNNNNNNGC 2 cut(s) 398, 1196
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 657
NcoI CCATGG 1 cut(s) 6
NdeII GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
NlaIII CATG 8 cut(s) 10, 565, 572, 887, 1119, 1160, 1262, 1267
NlaIV GGNNCC 6 cut(s) 12, 88, 111, 365, 825, 941
NmeAIII GCCGAG 3 cut(s) 723, 913, 927
NmuCI GTSAC 1 cut(s) 513
NspI RCATGY 2 cut(s) 1119, 1160
PciI ACATGT 1 cut(s) 1115
PcsI WCGNNNNNNNCGW 3 cut(s) 500, 503, 509
PfeI GAWTC 3 cut(s) 145, 275, 1097
PflFI GACNNNGTC 1 cut(s) 1259
PflMI CCANNNNNTGG 1 cut(s) 1264
PleI GAGTC 4 cut(s) 550, 1080, 1185, 1220
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 4 cut(s) 550, 1080, 1185, 1220
PscI ACATGT 1 cut(s) 1115
Psp6I CCWGG 2 cut(s) 542, 720
PspFI CCCAGC 1 cut(s) 896
PspGI CCWGG 2 cut(s) 542, 720
PspN4I GGNNCC 6 cut(s) 12, 88, 111, 365, 825, 941
PspPI GGNCC 4 cut(s) 257, 824, 899, 939
PsyI GACNNNGTC 1 cut(s) 1259
RseI CAYNNNNRTG 2 cut(s) 885, 888
SaqAI TTAA 5 cut(s) 252, 294, 434, 729, 1074
Sau3AI GATC 9 cut(s) 169, 211, 238, 343, 472, 577, 750, 925, 1026
Sau96I GGNCC 4 cut(s) 257, 824, 899, 939
SchI GAGTC 4 cut(s) 550, 1080, 1186, 1220
ScrFI CCNGG 3 cut(s) 544, 657, 722
SfaNI GCATC 5 cut(s) 239, 461, 839, 960, 1208
SfcI CTRYAG 1 cut(s) 333
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 2 cut(s) 257, 824
SmiMI CAYNNNNRTG 2 cut(s) 885, 888
Sse9I AATT 6 cut(s) 91, 368, 521, 604, 616, 637
SsiI CCGC 6 cut(s) 84, 311, 381, 390, 695, 937
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 3 cut(s) 227, 242, 1281
StyD4I CCNGG 3 cut(s) 542, 655, 720
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 4 cut(s) 68, 290, 993, 1048
TaiI ACGT 4 cut(s) 408, 635, 1151, 1289
TaqI TCGA 2 cut(s) 1051, 1089
TasI AATT 6 cut(s) 91, 368, 521, 604, 616, 637
TauI GCSGC 3 cut(s) 384, 697, 940
TfiI GAWTC 3 cut(s) 145, 275, 1097
Tru1I TTAA 5 cut(s) 252, 294, 434, 729, 1074
Tru9I TTAA 5 cut(s) 252, 294, 434, 729, 1074
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 513
TseI GCWGC 8 cut(s) 81, 539, 1129, 1153, 1190, 1193, 1196, 1270
Tsp45I GTSAC 1 cut(s) 513
TspDTI ATGAA 7 cut(s) 158, 288, 354, 432, 557, 638, 869
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 1 cut(s) 403
Tth111I GACNNNGTC 1 cut(s) 1259
Van91I CCANNNNNTGG 1 cut(s) 1264
VpaK11BI GGWCC 2 cut(s) 257, 824
XceI RCATGY 2 cut(s) 1119, 1160
XspI CTAG 3 cut(s) 227, 242, 1281
ZraI GACGTC 2 cut(s) 406, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.