Rw4G025100

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006209
Physical Location & Seq
Reverse (-)
51096344 .. 51105395
9052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025100.1

Sequence Viewer

Length: 1308 bp
ATGACCATGGTTCCTTCTGCACATCATCAAAACTTATCCAGACAACAACAACATCCTCATCTCTACGGTGGTTATGGTGGCTGCGGTGCCAATTATTACAACAATACGGCGCCTGACGACGAGAGCTTTGGTGGTCATCTTATGAATCAAGTAGATGAAGGTGGTTATGATCAGTATAGTTATTGTAATAACAATAATAATACTATTACTGATCAATCTTACTTATTAGCATCTTCCGATCTAGTAGTGGTTAATGGTCCGATGGCCGAAGATGAATCAAGAACTAACAGTCTTAACAACGAAGCCGAAGCCGAAGCGGGGTCAAGTTCTAAAGCTCCTGTAGATGAAGATCAGAGAGACGAGAGATGGCTCCAATTGAGTATAGGCGGCCACACCGCTACCAGTGCAGACGTCCAAGCTCCGGAAAGAAGAAGCTCGTTAAGGGGTTCATCATCGGCATCCGGATTGATTGAGCTTGATCTGTTATCAGCAAATATATCGTCGCCGTCGTCGTCATCGTCACGGCAATTTCAGTTCCAGGTCTTCCACGACTCCATGTTTCATGTTCCCGATCATCATCATCAAATTCGTGTTCCTAATTTCGGCACTAATTTGATGAACACTACGTCTAATTTATATTTTCAACACCCCGGAATGGGAATGGGAATGGGAATGAGTAGTTCTTTGCCGCCGAGCTTTCCTCAACACCAGCACCAGGAGGTTAACTGGGGATTTAGGCCATTGATCCCTCATCATAATAATCATATAGGGAATAGTGCTTCAACTTCTACCTCTTCCTCTTCTTTTATGCAACTGGGACCAGCATCCTCCTCCTATTTTGCACGCCCATTTCAACTTCAACATCATCATCTTCATCATGGTGGTGGTGCTGGGCCGAGCTTAGAATTCAGAGTTGTTGATCCTCCTCGGCGGCCCCAGTCTGGCATCTGGTTTATGCTACAAGCCTCACAAAATCAAGAAAAAGAACCGTTCTTGCCCCAGATACCTAAAAGCTACCTCAGGATCAAGGATGGAAGGATGACAGTTCGATTGATAAAGAAGTATCTGGTAAATAAGCTAAGACTCGACAGCGAATCAGAGGTAGAGATAACATGTAGAGGACAGCAGCTCCTACCTTTCTTGACGTTGCAGCATGTAAGAGATAATATATGGAGTCTAAGGGACGCAGCAGCAGCATCACTCACTTTGCTTCCACTTCCAGACTCCTCCTCCTCCACATCAACCACAGATGACCATGTCATGGTGCTGCACTATGCTAGGACGTCCTCGTCTTCTACAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

435

Amino Acids

48.1

Weight (kDa)

6.12

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 346 - 395 7.4e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1288
AatII GACGTC 2 cut(s) 414, 1286
AccB1I GGYRCC 2 cut(s) 86, 109
AccB7I CCANNNNNTGG 1 cut(s) 1261
AccIII TCCGGA 2 cut(s) 421, 461
AciI CCGC 6 cut(s) 84, 317, 387, 396, 689, 931
AclWI GGATC 3 cut(s) 739, 914, 1031
AcoI YGGCCR 2 cut(s) 264, 388
AcsI RAATTY 2 cut(s) 585, 905
AcyI GRCGYC 3 cut(s) 110, 411, 1283
AfiI CCNNNNNNNGG 8 cut(s) 318, 421, 602, 655, 656, 715, 941, 1261
AflIII ACRYGT 1 cut(s) 1112
AgsI TTSAA 4 cut(s) 644, 783, 854, 860
AjnI CCWGG 2 cut(s) 537, 714
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 3 cut(s) 739, 914, 1031
Aor13HI TCCGGA 2 cut(s) 421, 461
AoxI GGCC 5 cut(s) 264, 388, 737, 893, 932
ApeKI GCWGC 7 cut(s) 81, 1126, 1150, 1187, 1190, 1193, 1267
ApoI RAATTY 2 cut(s) 585, 905
ArsI GACNNNNNNTTYG 4 cut(s) 110, 142, 485, 517
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 4 cut(s) 257, 818, 893, 933
AsuC2I CCSGG 1 cut(s) 651
AvaII GGWCC 2 cut(s) 257, 818
AxyI CCTNAGG 1 cut(s) 1019
BanI GGYRCC 2 cut(s) 86, 109
BbsI GAAGAC 2 cut(s) 535, 1284
BbvI GCAGC 7 cut(s) 68, 1138, 1162, 1199, 1202, 1205, 1254
BccI CCATC 3 cut(s) 256, 360, 1025
BceAI ACGGC 3 cut(s) 123, 490, 539
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 2 cut(s) 539, 716
BclI TGATCA 2 cut(s) 169, 211
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 1 cut(s) 351
BfaI CTAG 2 cut(s) 242, 1278
BfmI CTRYAG 1 cut(s) 339
BfoI RGCGCY 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 539, 651, 716
Bme18I GGWCC 2 cut(s) 257, 818
BmgT120I GGNCC 4 cut(s) 257, 818, 893, 933
BmiI GGNNCC 6 cut(s) 12, 88, 111, 371, 819, 935
BmrFI CCNGG 3 cut(s) 539, 651, 716
BmrI ACTGGG 3 cut(s) 736, 824, 931
BmsI GCATC 5 cut(s) 239, 467, 833, 954, 1205
BmuI ACTGGG 3 cut(s) 736, 824, 931
BpiI GAAGAC 2 cut(s) 535, 1284
BplI GAGNNNNNCTC 2 cut(s) 685, 717
BpuMI CCSGG 1 cut(s) 651
BsaBI GATNNNNATC 2 cut(s) 348, 576
BsaHI GRCGYC 3 cut(s) 110, 411, 1283
BsaJI CCNNGG 3 cut(s) 6, 649, 926
BsaWI WCCGGW 2 cut(s) 421, 461
BsaXI ACNNNNNCTCC 4 cut(s) 1113, 1143, 1214, 1244
Bsc4I CCNNNNNNNGG 8 cut(s) 318, 421, 602, 655, 656, 715, 941, 1261
Bse1I ACTGG 4 cut(s) 402, 731, 819, 937
Bse21I CCTNAGG 1 cut(s) 1019
Bse8I GATNNNNATC 2 cut(s) 348, 576
BseAI TCCGGA 2 cut(s) 421, 461
BseBI CCWGG 2 cut(s) 539, 716
BseDI CCNNGG 3 cut(s) 6, 649, 926
BseGI GGATG 5 cut(s) 52, 458, 824, 1036, 1044
BseJI GATNNNNATC 2 cut(s) 348, 576
BseLI CCNNNNNNNGG 8 cut(s) 318, 421, 602, 655, 656, 715, 941, 1261
BseMII CTCAG 1 cut(s) 1033
BseNI ACTGG 4 cut(s) 402, 731, 819, 937
BseRI GAGGAG 5 cut(s) 820, 915, 1216, 1219, 1222
BseXI GCAGC 7 cut(s) 68, 1138, 1162, 1199, 1202, 1205, 1254
BseYI CCCAGC 1 cut(s) 890
BsgI GTGCAG 2 cut(s) 426, 1253
BshFI GGCC 5 cut(s) 266, 390, 739, 895, 934
BshNI GGYRCC 2 cut(s) 86, 109
BsiSI CCGG 3 cut(s) 422, 462, 651
BslFI GGGAC 2 cut(s) 831, 1196
BslI CCNNNNNNNGG 8 cut(s) 318, 421, 602, 655, 656, 715, 941, 1261
BsmAI GTCTC 1 cut(s) 351
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 2 cut(s) 831, 1196
BsnI GGCC 5 cut(s) 266, 390, 739, 895, 934
Bsp13I TCCGGA 2 cut(s) 421, 461
Bsp143I GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 84, 317, 387, 396, 689, 931
BspANI GGCC 5 cut(s) 266, 390, 739, 895, 934
BspCNI CTCAG 1 cut(s) 1032
BspEI TCCGGA 2 cut(s) 421, 461
BspLI GGNNCC 6 cut(s) 12, 88, 111, 371, 819, 935
BspPI GGATC 3 cut(s) 739, 914, 1031
BspT107I GGYRCC 2 cut(s) 86, 109
BsrI ACTGG 4 cut(s) 402, 731, 819, 937
BssECI CCNNGG 3 cut(s) 6, 649, 926
BssMI GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
BssNI GRCGYC 3 cut(s) 110, 411, 1283
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 539, 716
Bst4CI ACNGT 4 cut(s) 68, 290, 990, 1045
Bst6I CTCTTC 2 cut(s) 799, 805
BstACI GRCGYC 3 cut(s) 110, 411, 1283
BstC8I GCNNGC 1 cut(s) 844
BstDEI CTNAG 4 cut(s) 901, 1019, 1079, 1178
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 5 cut(s) 52, 458, 824, 1036, 1044
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 9 cut(s) 172, 214, 241, 352, 481, 574, 747, 922, 1026
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
BstMWI GCNNNNNNNGC 2 cut(s) 404, 1193
BstNI CCWGG 2 cut(s) 539, 716
BstNSI RCATGY 2 cut(s) 1116, 1157
BstSCI CCNGG 3 cut(s) 537, 649, 714
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 7 cut(s) 68, 1138, 1162, 1199, 1202, 1205, 1254
BstV2I GAAGAC 2 cut(s) 535, 1284
Bsu36I CCTNAGG 1 cut(s) 1019
BsuRI GGCC 5 cut(s) 266, 390, 739, 895, 934
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 5 cut(s) 52, 458, 824, 1036, 1044
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 844
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 257, 818, 893, 933
CseI GACGC 1 cut(s) 1193
CviAII CATG 8 cut(s) 7, 556, 563, 878, 1113, 1154, 1256, 1261
DdeI CTNAG 4 cut(s) 901, 1019, 1079, 1178
DinI GGCGCC 1 cut(s) 111
DpnI GATC 9 cut(s) 171, 213, 240, 351, 480, 573, 746, 921, 1025
DpnII GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
DrdI GACNNNNNNGTC 1 cut(s) 1288
DseDI GACNNNNNNGTC 1 cut(s) 1288
EaeI YGGCCR 2 cut(s) 264, 388
Eam1104I CTCTTC 2 cut(s) 799, 805
EarI CTCTTC 2 cut(s) 799, 805
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 257, 818
Eco81I CCTNAGG 1 cut(s) 1019
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 2 cut(s) 537, 714
EcoT14I CCWWGG 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 8 cut(s) 10, 559, 566, 881, 1116, 1157, 1259, 1264
FaqI GGGAC 2 cut(s) 831, 1196
FatI CATG 8 cut(s) 6, 555, 562, 877, 1112, 1153, 1255, 1260
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 2 cut(s) 169, 211
FokI GGATG 5 cut(s) 39, 445, 811, 1043, 1051
FspBI CTAG 2 cut(s) 242, 1278
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 894
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 5 cut(s) 266, 390, 739, 895, 934
HapII CCGG 3 cut(s) 422, 462, 651
HgaI GACGC 1 cut(s) 1193
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 3 cut(s) 110, 411, 1283
Hin1II CATG 8 cut(s) 10, 559, 566, 881, 1116, 1157, 1259, 1264
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HinfI GANTC 7 cut(s) 145, 275, 551, 1083, 1094, 1174, 1223
HpaI GTTAAC 1 cut(s) 724
HpaII CCGG 3 cut(s) 422, 462, 651
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 5 cut(s) 238, 261, 354, 911, 1099
Hpy188III TCNNGA 9 cut(s) 39, 279, 422, 462, 569, 977, 1021, 1141, 1220
Hpy8I GTNNAC 1 cut(s) 724
Hpy99I CGWCG 4 cut(s) 122, 505, 511, 514
HpyAV CCTTC 3 cut(s) 24, 152, 1029
HpyCH4III ACNGT 4 cut(s) 68, 290, 990, 1045
HpyCH4IV ACGT 4 cut(s) 411, 626, 1145, 1283
HpyCH4V TGCA 6 cut(s) 20, 407, 811, 842, 1150, 1270
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 1193
HpyF3I CTNAG 4 cut(s) 901, 1019, 1079, 1178
HpySE526I ACGT 4 cut(s) 411, 626, 1145, 1283
Hsp92I GRCGYC 3 cut(s) 110, 411, 1283
Hsp92II CATG 8 cut(s) 10, 559, 566, 881, 1116, 1157, 1259, 1264
HspAI GCGC 1 cut(s) 110
KasI GGCGCC 1 cut(s) 109
Kpn2I TCCGGA 2 cut(s) 421, 461
Ksp22I TGATCA 2 cut(s) 169, 211
KspAI GTTAAC 1 cut(s) 724
Kzo9I GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
LmnI GCTCC 4 cut(s) 340, 375, 424, 1134
Lsp1109I GCAGC 7 cut(s) 68, 1138, 1162, 1199, 1202, 1205, 1254
LweI GCATC 5 cut(s) 239, 467, 833, 954, 1205
MaeI CTAG 2 cut(s) 242, 1278
MaeII ACGT 4 cut(s) 411, 626, 1145, 1283
MaeIII GTNAC 1 cut(s) 519
MalI GATC 9 cut(s) 171, 213, 240, 351, 480, 573, 746, 921, 1025
MboI GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
MboII GAAGA 9 cut(s) 225, 281, 359, 441, 535, 786, 792, 863, 1284
MfeI CAATTG 1 cut(s) 374
MluCI AATT 8 cut(s) 91, 374, 527, 585, 598, 610, 631, 905
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 4 cut(s) 545, 1077, 1183, 1217
MroI TCCGGA 2 cut(s) 421, 461
MseI TTAA 4 cut(s) 252, 294, 440, 723
MslI CAYNNNNRTG 2 cut(s) 879, 882
MspI CCGG 3 cut(s) 422, 462, 651
MspR9I CCNGG 3 cut(s) 539, 651, 716
MunI CAATTG 1 cut(s) 374
MvaI CCWGG 2 cut(s) 539, 716
MwoI GCNNNNNNNGC 2 cut(s) 404, 1193
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 651
NcoI CCATGG 1 cut(s) 6
NdeII GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
NlaIII CATG 8 cut(s) 10, 559, 566, 881, 1116, 1157, 1259, 1264
NlaIV GGNNCC 6 cut(s) 12, 88, 111, 371, 819, 935
NmeAIII GCCGAG 3 cut(s) 717, 907, 921
NmuCI GTSAC 1 cut(s) 519
NspI RCATGY 2 cut(s) 1116, 1157
PciI ACATGT 1 cut(s) 1112
PcsI WCGNNNNNNNCGW 3 cut(s) 506, 509, 515
PfeI GAWTC 3 cut(s) 145, 275, 1094
PflFI GACNNNGTC 1 cut(s) 1256
PflMI CCANNNNNTGG 1 cut(s) 1261
PleI GAGTC 4 cut(s) 545, 1077, 1182, 1217
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 4 cut(s) 545, 1077, 1182, 1217
PscI ACATGT 1 cut(s) 1112
Psp6I CCWGG 2 cut(s) 537, 714
PspFI CCCAGC 1 cut(s) 890
PspGI CCWGG 2 cut(s) 537, 714
PspN4I GGNNCC 6 cut(s) 12, 88, 111, 371, 819, 935
PspPI GGNCC 4 cut(s) 257, 818, 893, 933
PsyI GACNNNGTC 1 cut(s) 1256
RseI CAYNNNNRTG 2 cut(s) 879, 882
SaqAI TTAA 4 cut(s) 252, 294, 440, 723
Sau3AI GATC 9 cut(s) 169, 211, 238, 349, 478, 571, 744, 919, 1023
Sau96I GGNCC 4 cut(s) 257, 818, 893, 933
SchI GAGTC 4 cut(s) 545, 1077, 1183, 1217
ScrFI CCNGG 3 cut(s) 539, 651, 716
SfaNI GCATC 5 cut(s) 239, 467, 833, 954, 1205
SfcI CTRYAG 1 cut(s) 339
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 2 cut(s) 257, 818
SmiMI CAYNNNNRTG 2 cut(s) 879, 882
Sse9I AATT 8 cut(s) 91, 374, 527, 585, 598, 610, 631, 905
SsiI CCGC 6 cut(s) 84, 317, 387, 396, 689, 931
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 2 cut(s) 242, 1278
StyD4I CCNGG 3 cut(s) 537, 649, 714
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 4 cut(s) 68, 290, 990, 1045
TaiI ACGT 4 cut(s) 414, 629, 1148, 1286
TaqI TCGA 2 cut(s) 1048, 1086
TasI AATT 8 cut(s) 91, 374, 527, 585, 598, 610, 631, 905
TauI GCSGC 3 cut(s) 390, 691, 934
TfiI GAWTC 3 cut(s) 145, 275, 1094
Tru1I TTAA 4 cut(s) 252, 294, 440, 723
Tru9I TTAA 4 cut(s) 252, 294, 440, 723
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 7 cut(s) 81, 1126, 1150, 1187, 1190, 1193, 1267
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 8 cut(s) 158, 171, 288, 360, 438, 551, 632, 863
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 1256
Van91I CCANNNNNTGG 1 cut(s) 1261
VpaK11BI GGWCC 2 cut(s) 257, 818
XapI RAATTY 2 cut(s) 585, 905
XceI RCATGY 2 cut(s) 1116, 1157
XspI CTAG 2 cut(s) 242, 1278
ZraI GACGTC 2 cut(s) 412, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.