Rroxscaffold_5G00370860

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006209
Physical Location & Seq
Reverse (-)
52512592 .. 52520435
7844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370860.1

Sequence Viewer

Length: 1323 bp
ATGACCATGGTTCCTTCTGCACATCATCAAAACTTATCCAGACAACAACAACATCCTCATCTCTACGGTGGTTATGGTGGCTGCGGTGCCAATTATTACAACAATACGGCGCCGGACGACGAGAGCTTTGGTGGTCATCTTATGAATCAAGTAGAGGAAGGTGGTTATGATCAGTATAGTTATTGTAATAACAATAATAATACTATTACTGATCAATCTTACTTATTGGCGTCTTCCGATCTAGTAGTGGTTAATGGTCCGATGGCCGAAGATGAATCAAGAACTAACAGTCTTAACAACGAAGCCGAAGCCGAAGCGGGTTCAAGTTCTAAAGCTCCTGTAGATGAAGACCAGAGAGACGAGAGATGGCTCCAATTGAGTATAGGCGGCCACACCGCTACCAGTGCAGACGTCCAAGCTCCCGAAAGAAGAAGCTCGTTAAGGGGTTCATCATCGGCATCCGGATTGATTGAGCTTGATCTGTTATCAGCAAATATATCGTCACCGTTACGGCAATTTCAGTTGCAACAAGCAGCCAGGTCTTCTATGACTCCCATGTTTCATGTTCCCGATCATCATCATCAAATTCGTGTTCCTAATTTCGGCACTAATTTGATGAACACTACGTCTAATTTATATTTTCAACACCCCGGAATCGGAATGGGAATGAGTAGTTCTTTGCCGCCGAGCTTTCCTCAACACCAGCACCAGGAGGTTAACTGGGGATTTAGGCCATTGATCCCTCATCATAATAATCATATAGGGAATAGTGCTTCAACTTCTACCTCTTCCTCTTCTTTTATGCAACTGGGACCAGCATCCTCCTCCTATTTTGCACGCCCATTTCAACTTCAACATCATCATCTTCATCATGGTGGTGGTGCTGGGCCGAGCTTAGATTTCAGAGTTGTTGATCCTCCTCGGCGGCCCCAGTCTGGCATCTGGTTTATGCTACAAGCCTCGCAAAATCACCGGTCTTTCCATCAATGCAGAGAAAAAGAACCGTTCTTGCCCCAGATACCTAAAAGCTACCTCAGGATCAAGGATGGAAGGATGACAGTTCGATTGATAAAGAAGTATCTGGTTAATAAGCTAAGACTCGACAGCGAATCAGAGGTAGAGATAACATGTAGAGGACAGCAGCTCCTACCTTTCTTGACGTTGCAGCATGTAAGAGATAATATATGGAGTCTAAGGGACGCAGCAGCAGCATCACTCACTTTGCTTCCACTTCCAGACTCCTCCTCCTCCACATCTACCACAGATGACCATGTCATGGTGCTGAACTATGCTAGGACGTCCTCGTCTTCTACAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

440

Amino Acids

48.78

Weight (kDa)

6.4

Isoelectric Point (pI)

58.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 351 - 400 7.5e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1303
AatII GACGTC 2 cut(s) 414, 1301
AccB1I GGYRCC 2 cut(s) 86, 109
AccB7I CCANNNNNTGG 1 cut(s) 1276
AccIII TCCGGA 1 cut(s) 461
AciI CCGC 6 cut(s) 84, 317, 387, 396, 683, 925
AclWI GGATC 3 cut(s) 733, 908, 1046
AcoI YGGCCR 2 cut(s) 264, 388
AcsI RAATTY 1 cut(s) 585
AcyI GRCGYC 4 cut(s) 110, 230, 411, 1298
AfiI CCNNNNNNNGG 5 cut(s) 602, 656, 709, 935, 1276
AflIII ACRYGT 1 cut(s) 1127
AgeI ACCGGT 1 cut(s) 972
AgsI TTSAA 5 cut(s) 324, 644, 777, 848, 854
AjnI CCWGG 2 cut(s) 536, 708
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 3 cut(s) 733, 908, 1046
Aor13HI TCCGGA 1 cut(s) 461
AoxI GGCC 5 cut(s) 264, 388, 731, 887, 926
ApeKI GCWGC 7 cut(s) 81, 533, 1141, 1165, 1202, 1205, 1208
ApoI RAATTY 1 cut(s) 585
ArsI GACNNNNNNTTYG 4 cut(s) 110, 142, 485, 517
AsiGI ACCGGT 1 cut(s) 972
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 4 cut(s) 257, 812, 887, 927
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 2 cut(s) 495, 962
AvaII GGWCC 2 cut(s) 257, 812
AxyI CCTNAGG 1 cut(s) 1034
BanI GGYRCC 2 cut(s) 86, 109
BbsI GAAGAC 4 cut(s) 225, 354, 534, 1299
BbvI GCAGC 7 cut(s) 68, 545, 1153, 1177, 1214, 1217, 1220
BccI CCATC 4 cut(s) 256, 360, 990, 1040
BceAI ACGGC 2 cut(s) 123, 527
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 2 cut(s) 538, 710
BclI TGATCA 2 cut(s) 169, 211
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 1 cut(s) 351
BfaI CTAG 2 cut(s) 242, 1293
BfmI CTRYAG 1 cut(s) 339
BfoI RGCGCY 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 538, 651, 710
Bme18I GGWCC 2 cut(s) 257, 812
BmgT120I GGNCC 4 cut(s) 257, 812, 887, 927
BmiI GGNNCC 6 cut(s) 12, 88, 111, 371, 813, 929
BmrFI CCNGG 3 cut(s) 538, 651, 710
BmrI ACTGGG 3 cut(s) 730, 818, 925
BmsI GCATC 4 cut(s) 467, 827, 948, 1220
BmuI ACTGGG 3 cut(s) 730, 818, 925
BpiI GAAGAC 4 cut(s) 225, 354, 534, 1299
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpuMI CCSGG 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 4 cut(s) 110, 230, 411, 1298
BsaJI CCNNGG 3 cut(s) 6, 649, 920
BsaWI WCCGGW 2 cut(s) 461, 972
BsaXI ACNNNNNCTCC 4 cut(s) 1128, 1158, 1229, 1259
Bsc4I CCNNNNNNNGG 5 cut(s) 602, 656, 709, 935, 1276
Bse118I RCCGGY 1 cut(s) 972
Bse1I ACTGG 4 cut(s) 402, 725, 813, 931
Bse21I CCTNAGG 1 cut(s) 1034
Bse8I GATNNNNATC 1 cut(s) 576
BseAI TCCGGA 1 cut(s) 461
BseBI CCWGG 2 cut(s) 538, 710
BseDI CCNNGG 3 cut(s) 6, 649, 920
BseGI GGATG 5 cut(s) 52, 458, 818, 1051, 1059
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 5 cut(s) 602, 656, 709, 935, 1276
BseMII CTCAG 1 cut(s) 1048
BseNI ACTGG 4 cut(s) 402, 725, 813, 931
BseRI GAGGAG 5 cut(s) 814, 909, 1231, 1234, 1237
BseXI GCAGC 7 cut(s) 68, 545, 1153, 1177, 1214, 1217, 1220
BseYI CCCAGC 1 cut(s) 884
BsgI GTGCAG 1 cut(s) 426
BshFI GGCC 5 cut(s) 266, 390, 733, 889, 928
BshNI GGYRCC 2 cut(s) 86, 109
BshTI ACCGGT 1 cut(s) 972
BsiSI CCGG 4 cut(s) 113, 462, 651, 973
BslFI GGGAC 2 cut(s) 825, 1211
BslI CCNNNNNNNGG 5 cut(s) 602, 656, 709, 935, 1276
BsmAI GTCTC 1 cut(s) 351
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 2 cut(s) 825, 1211
BsnI GGCC 5 cut(s) 266, 390, 733, 889, 928
Bsp13I TCCGGA 1 cut(s) 461
Bsp143I GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 84, 317, 387, 396, 683, 925
BspANI GGCC 5 cut(s) 266, 390, 733, 889, 928
BspCNI CTCAG 1 cut(s) 1047
BspEI TCCGGA 1 cut(s) 461
BspLI GGNNCC 6 cut(s) 12, 88, 111, 371, 813, 929
BspPI GGATC 3 cut(s) 733, 908, 1046
BspT107I GGYRCC 2 cut(s) 86, 109
BsrFI RCCGGY 1 cut(s) 972
BsrI ACTGG 4 cut(s) 402, 725, 813, 931
BssAI RCCGGY 1 cut(s) 972
BssECI CCNNGG 3 cut(s) 6, 649, 920
BssMI GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
BssNI GRCGYC 4 cut(s) 110, 230, 411, 1298
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 538, 710
Bst4CI ACNGT 5 cut(s) 68, 290, 507, 1005, 1060
Bst6I CTCTTC 2 cut(s) 793, 799
BstACI GRCGYC 4 cut(s) 110, 230, 411, 1298
BstC8I GCNNGC 1 cut(s) 838
BstDEI CTNAG 4 cut(s) 895, 1034, 1094, 1193
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 5 cut(s) 52, 458, 818, 1051, 1059
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 8 cut(s) 172, 214, 241, 481, 574, 741, 916, 1041
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
BstMWI GCNNNNNNNGC 2 cut(s) 404, 1208
BstNI CCWGG 2 cut(s) 538, 710
BstNSI RCATGY 2 cut(s) 1131, 1172
BstSCI CCNGG 3 cut(s) 536, 649, 708
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 7 cut(s) 68, 545, 1153, 1177, 1214, 1217, 1220
BstV2I GAAGAC 4 cut(s) 225, 354, 534, 1299
Bsu36I CCTNAGG 1 cut(s) 1034
BsuRI GGCC 5 cut(s) 266, 390, 733, 889, 928
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 5 cut(s) 52, 458, 818, 1051, 1059
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 838
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 972
Cfr13I GGNCC 4 cut(s) 257, 812, 887, 927
CseI GACGC 2 cut(s) 219, 1208
CspAI ACCGGT 1 cut(s) 972
CviAII CATG 8 cut(s) 7, 556, 563, 872, 1128, 1169, 1271, 1276
DdeI CTNAG 4 cut(s) 895, 1034, 1094, 1193
DinI GGCGCC 1 cut(s) 111
DpnI GATC 8 cut(s) 171, 213, 240, 480, 573, 740, 915, 1040
DpnII GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
DrdI GACNNNNNNGTC 1 cut(s) 1303
DseDI GACNNNNNNGTC 1 cut(s) 1303
EaeI YGGCCR 2 cut(s) 264, 388
Eam1104I CTCTTC 2 cut(s) 793, 799
EarI CTCTTC 2 cut(s) 793, 799
Eco130I CCWWGG 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 257, 812
Eco81I CCTNAGG 1 cut(s) 1034
EcoRII CCWGG 2 cut(s) 536, 708
EcoT14I CCWWGG 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 8 cut(s) 10, 559, 566, 875, 1131, 1172, 1274, 1279
FaqI GGGAC 2 cut(s) 825, 1211
FatI CATG 8 cut(s) 6, 555, 562, 871, 1127, 1168, 1270, 1275
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 2 cut(s) 169, 211
FokI GGATG 5 cut(s) 39, 445, 805, 1058, 1066
FspBI CTAG 2 cut(s) 242, 1293
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 5 cut(s) 266, 390, 733, 889, 928
HapII CCGG 4 cut(s) 113, 462, 651, 973
HgaI GACGC 2 cut(s) 219, 1208
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 4 cut(s) 110, 230, 411, 1298
Hin1II CATG 8 cut(s) 10, 559, 566, 875, 1131, 1172, 1274, 1279
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HinfI GANTC 8 cut(s) 145, 275, 550, 654, 1098, 1109, 1189, 1238
HpaI GTTAAC 1 cut(s) 718
HpaII CCGG 4 cut(s) 113, 462, 651, 973
HphI GGTGA 2 cut(s) 495, 962
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 5 cut(s) 238, 261, 659, 905, 1114
Hpy188III TCNNGA 8 cut(s) 39, 279, 422, 462, 569, 1036, 1156, 1235
Hpy8I GTNNAC 1 cut(s) 718
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 24, 152, 1044
HpyCH4III ACNGT 5 cut(s) 68, 290, 507, 1005, 1060
HpyCH4IV ACGT 4 cut(s) 411, 626, 1160, 1298
HpyCH4V TGCA 7 cut(s) 20, 407, 526, 805, 836, 990, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 1208
HpyF3I CTNAG 4 cut(s) 895, 1034, 1094, 1193
HpySE526I ACGT 4 cut(s) 411, 626, 1160, 1298
Hsp92I GRCGYC 4 cut(s) 110, 230, 411, 1298
Hsp92II CATG 8 cut(s) 10, 559, 566, 875, 1131, 1172, 1274, 1279
HspAI GCGC 1 cut(s) 110
KasI GGCGCC 1 cut(s) 109
Kpn2I TCCGGA 1 cut(s) 461
Ksp22I TGATCA 2 cut(s) 169, 211
KspAI GTTAAC 1 cut(s) 718
Kzo9I GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
LmnI GCTCC 4 cut(s) 340, 375, 424, 1149
Lsp1109I GCAGC 7 cut(s) 68, 545, 1153, 1177, 1214, 1217, 1220
LweI GCATC 4 cut(s) 467, 827, 948, 1220
MaeI CTAG 2 cut(s) 242, 1293
MaeII ACGT 4 cut(s) 411, 626, 1160, 1298
MaeIII GTNAC 2 cut(s) 501, 507
MalI GATC 8 cut(s) 171, 213, 240, 480, 573, 740, 915, 1040
MboI GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
MboII GAAGA 9 cut(s) 225, 281, 359, 441, 534, 780, 786, 857, 1299
MfeI CAATTG 1 cut(s) 374
MluCI AATT 7 cut(s) 91, 374, 515, 585, 598, 610, 631
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 4 cut(s) 544, 1092, 1198, 1232
MroI TCCGGA 1 cut(s) 461
MseI TTAA 5 cut(s) 252, 294, 440, 717, 1086
MslI CAYNNNNRTG 2 cut(s) 873, 876
MspI CCGG 4 cut(s) 113, 462, 651, 973
MspR9I CCNGG 3 cut(s) 538, 651, 710
MunI CAATTG 1 cut(s) 374
MvaI CCWGG 2 cut(s) 538, 710
MwoI GCNNNNNNNGC 2 cut(s) 404, 1208
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 651
NcoI CCATGG 1 cut(s) 6
NdeII GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
NlaIII CATG 8 cut(s) 10, 559, 566, 875, 1131, 1172, 1274, 1279
NlaIV GGNNCC 6 cut(s) 12, 88, 111, 371, 813, 929
NmeAIII GCCGAG 3 cut(s) 711, 901, 915
NmuCI GTSAC 1 cut(s) 501
NspI RCATGY 2 cut(s) 1131, 1172
PciI ACATGT 1 cut(s) 1127
PfeI GAWTC 4 cut(s) 145, 275, 654, 1109
PflFI GACNNNGTC 1 cut(s) 1271
PflMI CCANNNNNTGG 1 cut(s) 1276
PinAI ACCGGT 1 cut(s) 972
PleI GAGTC 4 cut(s) 544, 1092, 1197, 1232
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 4 cut(s) 544, 1092, 1197, 1232
PscI ACATGT 1 cut(s) 1127
Psp6I CCWGG 2 cut(s) 536, 708
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 2 cut(s) 536, 708
PspN4I GGNNCC 6 cut(s) 12, 88, 111, 371, 813, 929
PspPI GGNCC 4 cut(s) 257, 812, 887, 927
PsyI GACNNNGTC 1 cut(s) 1271
RseI CAYNNNNRTG 2 cut(s) 873, 876
SaqAI TTAA 5 cut(s) 252, 294, 440, 717, 1086
Sau3AI GATC 8 cut(s) 169, 211, 238, 478, 571, 738, 913, 1038
Sau96I GGNCC 4 cut(s) 257, 812, 887, 927
SchI GAGTC 4 cut(s) 544, 1092, 1198, 1232
ScrFI CCNGG 3 cut(s) 538, 651, 710
SfaNI GCATC 4 cut(s) 467, 827, 948, 1220
SfcI CTRYAG 1 cut(s) 339
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 2 cut(s) 257, 812
SmiMI CAYNNNNRTG 2 cut(s) 873, 876
Sse9I AATT 7 cut(s) 91, 374, 515, 585, 598, 610, 631
SsiI CCGC 6 cut(s) 84, 317, 387, 396, 683, 925
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 2 cut(s) 242, 1293
StyD4I CCNGG 3 cut(s) 536, 649, 708
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 5 cut(s) 68, 290, 507, 1005, 1060
TaiI ACGT 4 cut(s) 414, 629, 1163, 1301
TaqI TCGA 2 cut(s) 1063, 1101
TasI AATT 7 cut(s) 91, 374, 515, 585, 598, 610, 631
TauI GCSGC 3 cut(s) 390, 685, 928
TfiI GAWTC 4 cut(s) 145, 275, 654, 1109
Tru1I TTAA 5 cut(s) 252, 294, 440, 717, 1086
Tru9I TTAA 5 cut(s) 252, 294, 440, 717, 1086
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 501
TseI GCWGC 7 cut(s) 81, 533, 1141, 1165, 1202, 1205, 1208
Tsp45I GTSAC 1 cut(s) 501
TspDTI ATGAA 7 cut(s) 158, 288, 360, 438, 551, 632, 857
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 1271
Van91I CCANNNNNTGG 1 cut(s) 1276
VpaK11BI GGWCC 2 cut(s) 257, 812
XapI RAATTY 1 cut(s) 585
XceI RCATGY 2 cut(s) 1131, 1172
XspI CTAG 2 cut(s) 242, 1293
ZraI GACGTC 2 cut(s) 412, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.