MD16G1128300.v1.1

histone H2A-K119 monoubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006209
Physical Location & Seq
Forward (+)
9391294 .. 9401291
9998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1128300.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGTTCCTGCTGCTGCTCATCATCAAAACATATCCAACCAACAACAACACAATCTCTACGGCGTTTATGGTGGTTGTGGTGCCAATTACACGGCGGAGATCAGCAGTTTCGGTCTTATGAGTACTAGTCAAGTTGTAGAAGAAGGTTATGAGTATAACTATTATTGCAATAATATTGTTACTGAATCTGATCATCATCATCTAGCGGGAATTGATCCAATGGCCGAGGATGAGTCGAGAACTAACAGCGTAAACGAAGCGGGTTCGAGTTCTAGAGATGCTCCGGAAGATAACGAAACATGGCTCCAATTGACTATAGGCGGCGGCTCCAACAACAACCAACCTGCGACCGGGGAACATCCAACCCTCAGAAGCTTACCAAGTAGTACTACTCCTTCTGGTCCCGGATTGGTTGAACTTGATCTTGATCTGTTACCACCAGGCCATGATCACGGTGGTTTTTCGTCATTACGGCAAGTGAGGTCCATGCCACATATGCCTCCAATGTTTCATGTTCCTCAACATGAAAATATTCTCCGTGCTCCTCCTTCGAATCTGTCTTTCAGGCCTCCGTTTAATTCGAATTTTTCTTCGAGTAACGCTACGTCTTTATATTTCCAGCAACCGGGGCCAGGAATGAGTAGCTCCTCGTCCGGTTTATTTCCTCACCAGGAGGTTAACTGGGCATTTAGGCCAGCAATCCCTTATAATAATATAGGGATGATGGCTTCTACTTCTTTATCTTCTTCTGCTTCTTCTTTTATAGAACTGGGTTCATCCTATTTTGCACGCCCATTCCATCGTCCTCTTCGTGAATTTAATGTCACCGGGCCAAGCTTAGATTTCAGAGTTGTTGATGCTCCGAGAAGGCCCCAATCCGGCATTTGGTTTACGCTCCAAGCATCCCAAAATCAAGCAAAAGAACCTTTCTTGCCCCAGATACCCAAAAGCTACCTAAGAATCAAGGATGGAAAAATGACAGTTCGGTTGATAAAGAAGTATCTGGTTAATAAGCTGAGACTGGAAAGCGAATCAGAGGTAGAGATAACAATTAGAGGACAGGAGCTTCTACCTTTCTTGACGTTGCAGCATGTAAGAGATAACATCTGGAGTCGAAGGGACGCACTCACTTTGCTTCCAGACTCCTCAACCACTGATCATGTCATGGTACTGCACTATGCTAGGACGACGACAATTACGACGACGACTACAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

44.96

Weight (kDa)

6.09

Isoelectric Point (pI)

66.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAWUL PF16207 325 - 378 9.2e-07 RAWUL domain RING finger- and WD40-associated ubiquitin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
Acc36I ACCTGC 1 cut(s) 352
AccB1I GGYRCC 1 cut(s) 80
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 5 cut(s) 95, 206, 260, 321, 324
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 2 cut(s) 583, 815
AfaI GTAC 3 cut(s) 124, 388, 1170
AfiI CCNNNNNNNGG 5 cut(s) 350, 625, 632, 878, 885
AgsI TTSAA 1 cut(s) 416
AhlI ACTAGT 1 cut(s) 125
AjnI CCWGG 3 cut(s) 439, 631, 669
AluBI AGCT 6 cut(s) 375, 645, 837, 951, 1015, 1066
AluI AGCT 6 cut(s) 375, 645, 837, 951, 1015, 1066
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 1 cut(s) 1012
AlwI GGATC 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 7 cut(s) 222, 442, 566, 629, 692, 830, 869
ApeKI GCWGC 3 cut(s) 11, 14, 1087
ApoI RAATTY 2 cut(s) 583, 815
Asp700I GAANNNNTTC 1 cut(s) 531
AspS9I GGNCC 5 cut(s) 401, 483, 629, 830, 870
AsuC2I CCSGG 4 cut(s) 352, 405, 627, 829
AsuHPI GGTGA 2 cut(s) 659, 817
AsuII TTCGAA 2 cut(s) 551, 581
AvaII GGWCC 2 cut(s) 401, 483
BanI GGYRCC 1 cut(s) 80
BarI GAAGNNNNNNTAC 2 cut(s) 379, 411
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 1 cut(s) 1099
BccI CCATC 3 cut(s) 718, 807, 962
BceAI ACGGC 3 cut(s) 76, 108, 488
BciT130I CCWGG 3 cut(s) 441, 633, 671
BclI TGATCA 3 cut(s) 190, 448, 1156
BcnI CCSGG 4 cut(s) 352, 405, 627, 829
BcoDI GTCTC 1 cut(s) 1012
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 4 cut(s) 126, 203, 273, 1182
BfmI CTRYAG 1 cut(s) 315
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 5 cut(s) 12, 15, 322, 325, 1088
BlsI GCNGC 5 cut(s) 13, 16, 323, 326, 1089
BmcAI AGTACT 2 cut(s) 124, 388
Bme1390I CCNGG 7 cut(s) 352, 405, 441, 627, 633, 671, 829
Bme18I GGWCC 2 cut(s) 401, 483
BmgT120I GGNCC 5 cut(s) 401, 483, 629, 830, 870
BmiI GGNNCC 7 cut(s) 6, 82, 305, 328, 403, 630, 872
BmrFI CCNGG 7 cut(s) 352, 405, 441, 627, 633, 671, 829
BmrI ACTGGG 2 cut(s) 691, 779
BmsI GCATC 3 cut(s) 268, 847, 911
BmuI ACTGGG 2 cut(s) 691, 779
BpmI CTGGAG 1 cut(s) 1129
Bpu14I TTCGAA 2 cut(s) 551, 581
BpuMI CCSGG 4 cut(s) 352, 405, 627, 829
BsaBI GATNNNNATC 2 cut(s) 195, 426
BsaJI CCNNGG 3 cut(s) 225, 351, 626
BsaWI WCCGGW 2 cut(s) 283, 653
Bsc4I CCNNNNNNNGG 5 cut(s) 350, 625, 632, 878, 885
Bse1I ACTGG 3 cut(s) 686, 774, 1026
Bse8I GATNNNNATC 2 cut(s) 195, 426
BseAI TCCGGA 1 cut(s) 283
BseBI CCWGG 3 cut(s) 441, 633, 671
BseDI CCNNGG 3 cut(s) 225, 351, 626
BseGI GGATG 6 cut(s) 235, 358, 726, 776, 902, 973
BseJI GATNNNNATC 2 cut(s) 195, 426
BseLI CCNNNNNNNGG 5 cut(s) 350, 625, 632, 878, 885
BseMII CTCAG 2 cut(s) 382, 1007
BseNI ACTGG 3 cut(s) 686, 774, 1026
BseRI GAGGAG 3 cut(s) 534, 637, 1135
BseXI GCAGC 1 cut(s) 1099
BsgI GTGCAG 1 cut(s) 1157
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 7 cut(s) 224, 444, 568, 631, 694, 832, 871
BshNI GGYRCC 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 7 cut(s) 284, 351, 405, 626, 654, 828, 879
BslFI GGGAC 2 cut(s) 387, 1133
BslI CCNNNNNNNGG 5 cut(s) 350, 625, 632, 878, 885
BsmAI GTCTC 1 cut(s) 1012
BsmFI GGGAC 2 cut(s) 387, 1133
BsnI GGCC 7 cut(s) 224, 444, 568, 631, 694, 832, 871
Bsp119I TTCGAA 2 cut(s) 551, 581
Bsp1286I GDGCHC 1 cut(s) 544
Bsp13I TCCGGA 1 cut(s) 283
Bsp143I GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
BspACI CCGC 5 cut(s) 95, 206, 260, 321, 324
BspANI GGCC 7 cut(s) 224, 444, 568, 631, 694, 832, 871
BspCNI CTCAG 2 cut(s) 381, 1008
BspEI TCCGGA 1 cut(s) 283
BspLI GGNNCC 7 cut(s) 6, 82, 305, 328, 403, 630, 872
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 1 cut(s) 209
BspT104I TTCGAA 2 cut(s) 551, 581
BspT107I GGYRCC 1 cut(s) 80
BsrI ACTGG 3 cut(s) 686, 774, 1026
BssECI CCNNGG 3 cut(s) 225, 351, 626
BssMI GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
Bst2UI CCWGG 3 cut(s) 441, 633, 671
Bst4CI ACNGT 2 cut(s) 455, 982
Bst6I CTCTTC 1 cut(s) 813
BstBI TTCGAA 2 cut(s) 551, 581
BstC8I GCNNGC 2 cut(s) 696, 790
BstDEI CTNAG 4 cut(s) 368, 838, 956, 1016
BstF5I GGATG 6 cut(s) 235, 358, 726, 776, 902, 973
BstKTI GATC 7 cut(s) 102, 193, 217, 424, 430, 451, 1159
BstMAI GTCTC 1 cut(s) 1012
BstMBI GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 2 cut(s) 496, 628
BstNI CCWGG 3 cut(s) 441, 633, 671
BstNSI RCATGY 1 cut(s) 1094
BstSCI CCNGG 7 cut(s) 350, 403, 439, 625, 631, 669, 827
BstSFI CTRYAG 1 cut(s) 315
BstV1I GCAGC 1 cut(s) 1099
BsuRI GGCC 7 cut(s) 224, 444, 568, 631, 694, 832, 871
BtsCI GGATG 6 cut(s) 235, 358, 726, 776, 902, 973
BtsIMutI CAGTG 1 cut(s) 1152
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 696, 790
Cfr13I GGNCC 5 cut(s) 401, 483, 629, 830, 870
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 3 cut(s) 123, 387, 1169
CviAII CATG 8 cut(s) 300, 446, 487, 512, 524, 1091, 1160, 1165
CviQI GTAC 3 cut(s) 123, 387, 1169
DdeI CTNAG 4 cut(s) 368, 838, 956, 1016
DpnI GATC 7 cut(s) 101, 192, 216, 423, 429, 450, 1158
DpnII GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
EciI GGCGGA 1 cut(s) 110
Eco147I AGGCCT 1 cut(s) 568
Eco47I GGWCC 2 cut(s) 401, 483
EcoO109I RGGNCCY 1 cut(s) 870
EcoRII CCWGG 3 cut(s) 439, 631, 669
FaeI CATG 8 cut(s) 303, 449, 490, 515, 527, 1094, 1163, 1168
FaqI GGGAC 2 cut(s) 387, 1133
FatI CATG 8 cut(s) 299, 445, 486, 511, 523, 1090, 1159, 1164
FauI CCCGC 2 cut(s) 199, 253
FauNDI CATATG 1 cut(s) 495
FbaI TGATCA 3 cut(s) 190, 448, 1156
Fnu4HI GCNGC 5 cut(s) 12, 15, 322, 325, 1088
FokI GGATG 6 cut(s) 242, 345, 733, 763, 889, 980
Fsp4HI GCNGC 5 cut(s) 12, 15, 322, 325, 1088
FspBI CTAG 4 cut(s) 126, 203, 273, 1182
GluI GCNGC 5 cut(s) 12, 15, 322, 325, 1088
GsuI CTGGAG 1 cut(s) 1129
HaeIII GGCC 7 cut(s) 224, 444, 568, 631, 694, 832, 871
HapII CCGG 7 cut(s) 284, 351, 405, 626, 654, 828, 879
HgaI GACGC 1 cut(s) 1130
Hin1II CATG 8 cut(s) 303, 449, 490, 515, 527, 1094, 1163, 1168
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 2 cut(s) 373, 835
HinfI GANTC 7 cut(s) 185, 233, 553, 960, 1031, 1111, 1142
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 7 cut(s) 284, 351, 405, 626, 654, 828, 879
HphI GGTGA 2 cut(s) 659, 817
Hpy166II GTNNAC 3 cut(s) 253, 679, 891
Hpy188I TCNGA 5 cut(s) 190, 371, 848, 864, 1036
Hpy188III TCNNGA 8 cut(s) 237, 273, 284, 425, 812, 1078, 1108, 1139
Hpy8I GTNNAC 3 cut(s) 253, 679, 891
Hpy99I CGWCG 3 cut(s) 1192, 1204, 1207
HpyAV CCTTC 5 cut(s) 137, 405, 558, 861, 1110
HpyCH4III ACNGT 2 cut(s) 455, 982
HpyCH4IV ACGT 2 cut(s) 605, 1082
HpyCH4V TGCA 4 cut(s) 168, 788, 1087, 1174
HpyF10VI GCNNNNNNNGC 2 cut(s) 496, 628
HpyF3I CTNAG 4 cut(s) 368, 838, 956, 1016
HpySE526I ACGT 2 cut(s) 605, 1082
Hsp92II CATG 8 cut(s) 303, 449, 490, 515, 527, 1094, 1163, 1168
Kpn2I TCCGGA 1 cut(s) 283
Ksp22I TGATCA 3 cut(s) 190, 448, 1156
KspAI GTTAAC 1 cut(s) 679
Kzo9I GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
LmnI GCTCC 8 cut(s) 286, 309, 332, 547, 650, 865, 900, 1063
Lsp1109I GCAGC 1 cut(s) 1099
LweI GCATC 3 cut(s) 268, 847, 911
MaeI CTAG 4 cut(s) 126, 203, 273, 1182
MaeII ACGT 2 cut(s) 605, 1082
MaeIII GTNAC 4 cut(s) 178, 432, 596, 823
MalI GATC 7 cut(s) 101, 192, 216, 423, 429, 450, 1158
MboI GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
MboII GAAGA 7 cut(s) 152, 299, 582, 735, 738, 747, 800
MfeI CAATTG 1 cut(s) 308
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 8 cut(s) 85, 210, 308, 577, 583, 815, 1050, 1194
MlyI GAGTC 3 cut(s) 242, 1120, 1136
MmeI TCCRAC 3 cut(s) 60, 354, 386
MroI TCCGGA 1 cut(s) 283
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 4 cut(s) 576, 678, 819, 1008
MspI CCGG 7 cut(s) 284, 351, 405, 626, 654, 828, 879
MspR9I CCNGG 7 cut(s) 352, 405, 441, 627, 633, 671, 829
MunI CAATTG 1 cut(s) 308
MvaI CCWGG 3 cut(s) 441, 633, 671
MwoI GCNNNNNNNGC 2 cut(s) 496, 628
NciI CCSGG 4 cut(s) 352, 405, 627, 829
NdeI CATATG 1 cut(s) 495
NdeII GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
NlaIII CATG 8 cut(s) 303, 449, 490, 515, 527, 1094, 1163, 1168
NlaIV GGNNCC 7 cut(s) 6, 82, 305, 328, 403, 630, 872
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 823
NspI RCATGY 1 cut(s) 1094
NspV TTCGAA 2 cut(s) 551, 581
PceI AGGCCT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 2 cut(s) 808, 1196
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 4 cut(s) 185, 553, 960, 1031
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 5 cut(s) 13, 16, 323, 326, 1089
PleI GAGTC 3 cut(s) 241, 1119, 1136
PpsI GAGTC 3 cut(s) 241, 1119, 1136
PsiI TTATAA 1 cut(s) 708
Psp6I CCWGG 3 cut(s) 439, 631, 669
PspGI CCWGG 3 cut(s) 439, 631, 669
PspN4I GGNNCC 7 cut(s) 6, 82, 305, 328, 403, 630, 872
PspPI GGNCC 5 cut(s) 401, 483, 629, 830, 870
RsaI GTAC 3 cut(s) 124, 388, 1170
RsaNI GTAC 3 cut(s) 123, 387, 1169
SaqAI TTAA 4 cut(s) 576, 678, 819, 1008
SatI GCNGC 5 cut(s) 12, 15, 322, 325, 1088
Sau3AI GATC 7 cut(s) 99, 190, 214, 421, 427, 448, 1156
Sau96I GGNCC 5 cut(s) 401, 483, 629, 830, 870
ScaI AGTACT 2 cut(s) 124, 388
SchI GAGTC 3 cut(s) 242, 1120, 1136
ScrFI CCNGG 7 cut(s) 352, 405, 441, 627, 633, 671, 829
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 3 cut(s) 268, 847, 911
SfcI CTRYAG 1 cut(s) 315
SfuI TTCGAA 2 cut(s) 551, 581
SinI GGWCC 2 cut(s) 401, 483
SpeI ACTAGT 1 cut(s) 125
Sse9I AATT 8 cut(s) 85, 210, 308, 577, 583, 815, 1050, 1194
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 5 cut(s) 95, 206, 260, 321, 324
SspI AATATT 2 cut(s) 175, 532
SspMI CTAG 4 cut(s) 126, 203, 273, 1182
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 7 cut(s) 350, 403, 439, 625, 631, 669, 827
TaaI ACNGT 2 cut(s) 455, 982
TaiI ACGT 2 cut(s) 608, 1085
TaqI TCGA 6 cut(s) 236, 266, 551, 581, 593, 1114
TaqII GACCGA 1 cut(s) 101
TasI AATT 8 cut(s) 85, 210, 308, 577, 583, 815, 1050, 1194
TatI WGTACW 2 cut(s) 122, 386
TauI GCSGC 2 cut(s) 324, 327
TfiI GAWTC 4 cut(s) 185, 553, 960, 1031
Tru1I TTAA 4 cut(s) 576, 678, 819, 1008
Tru9I TTAA 4 cut(s) 576, 678, 819, 1008
TscAI CASTG 1 cut(s) 1159
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 3 cut(s) 11, 14, 1087
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 3 cut(s) 500, 540, 765
TspGWI ACGGA 2 cut(s) 527, 561
TspRI CASTG 1 cut(s) 1159
VpaK11BI GGWCC 2 cut(s) 401, 483
XapI RAATTY 2 cut(s) 583, 815
XbaI TCTAGA 1 cut(s) 272
XceI RCATGY 1 cut(s) 1094
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 4 cut(s) 126, 203, 273, 1182
ZrmI AGTACT 2 cut(s) 124, 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.