AT5G14285

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004398
Physical Location & Seq
Forward (+)
4610567 .. 4611658
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14285.1

Sequence Viewer

Length: 765 bp
ATGTCGATCAATATCGGAGAAATCTCAATTCCAGACCTTCCTATCTTCTTCTCCATGTTCTTAACCATCTACCTAATCGCTTACTTCATCGTCTTCCGCAATTGGAAACCACAAATCCGTCCCGAAGCTTCAAGCTGCCTGATCTCAATCTTCCATGGATCTCCAGCGGTTTTCCTCGCCACACGCGCAGTTTTCTCCTCCTCCGAACGCTCCTTCGCCTCCGCAAACACCGCCGCACAAAACACCGTCCTCGATTTCAGCGTCGCTTACTTCCTCACCGATCTCTTCCACTACATCGTCTTCAACCCTAACGACGTACTCTTCATCGGCCATCACGTAGCCACTCTATTCGTCTTCCTAACCTGCAGATTCCTCGTCTTCCACGGGGCTTGCGCCATCTTAGGCCTCCTGATTCTAGCTGAAGTAACAAGCGCGTGTCAGAACGCGTGGACGCTCGCCGGAGCAAGGAAGAATGATCCGGAATCACGATTAGCTGTGAAAGTGTACGATCTCTTGTCTCCTCCGTTTTACGCCTTCTACAGTATCGTTAGGGGTGTGTTGGGACCTTTGTTTTTCGGGAAAATGGTAGCGTTTTACGCTCGAGGTGGTGCTCATGGTGTGATACCTAATTGGTTGTGGATTTCTTGGGCAATTGTTGTTGGAATTGCTATTACTGTTAGTATACTTTGGATTTGGAATCTCTGGATAGAATTGTTTAGTGAAAGGAAAGCTAACAAAATCAGAGTAGACAAGAAAATTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

254

Amino Acids

28.6

Weight (kDa)

9.25

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 44 - 235 8.4e-23 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 371
AccI GTMKAC 2 cut(s) 682, 747
AccII CGCG 3 cut(s) 186, 434, 446
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 5 cut(s) 97, 167, 222, 231, 234
AclWI GGATC 2 cut(s) 166, 470
AcoI YGGCCR 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 318, 506
AfiI CCNNNNNNNGG 1 cut(s) 465
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 2 cut(s) 132, 304
AluBI AGCT 5 cut(s) 128, 135, 419, 494, 731
AluI AGCT 5 cut(s) 128, 135, 419, 494, 731
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 2 cut(s) 166, 470
Ama87I CYCGRG 1 cut(s) 600
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 2 cut(s) 328, 403
ApeKI GCWGC 1 cut(s) 135
AspLEI GCGC 3 cut(s) 188, 395, 434
AspS9I GGNCC 1 cut(s) 563
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 600
AvaII GGWCC 1 cut(s) 563
BbsI GAAGAC 4 cut(s) 85, 292, 346, 370
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 1 cut(s) 122
BccI CCATC 3 cut(s) 74, 339, 404
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 2 cut(s) 364, 538
BfuAI ACCTGC 1 cut(s) 371
BisI GCNGC 2 cut(s) 136, 234
BlsI GCNGC 2 cut(s) 137, 235
Bme18I GGWCC 1 cut(s) 563
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 1 cut(s) 564
BpiI GAAGAC 4 cut(s) 85, 292, 346, 370
BpmI CTGGAG 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 2 cut(s) 11, 146
BsaJI CCNNGG 2 cut(s) 154, 382
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse8I GATNNNNATC 2 cut(s) 11, 146
BseAI TCCGGA 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 154, 382
BseJI GATNNNNATC 2 cut(s) 11, 146
BseLI CCNNNNNNNGG 1 cut(s) 465
BseRI GAGGAG 3 cut(s) 187, 190, 510
BseXI GCAGC 1 cut(s) 122
Bsh1236I CGCG 3 cut(s) 186, 434, 446
BshFI GGCC 2 cut(s) 330, 405
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 2 cut(s) 459, 479
BslFI GGGAC 2 cut(s) 105, 576
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 2 cut(s) 105, 576
BsnI GGCC 2 cut(s) 330, 405
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 1 cut(s) 613
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 6 cut(s) 6, 141, 158, 280, 475, 508
Bsp19I CCATGG 1 cut(s) 154
BspACI CCGC 5 cut(s) 97, 167, 222, 231, 234
BspANI GGCC 2 cut(s) 330, 405
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 3 cut(s) 186, 434, 446
BspLI GGNNCC 1 cut(s) 564
BspMAI CTGCAG 1 cut(s) 368
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 166, 470
BssECI CCNNGG 2 cut(s) 154, 382
BssMI GATC 6 cut(s) 6, 141, 158, 280, 475, 508
BssNAI GTATAC 1 cut(s) 683
BssT1I CCWWGG 1 cut(s) 154
Bst1107I GTATAC 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 247, 542, 676
Bst6I CTCTTC 2 cut(s) 290, 326
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 391, 456
BstDEI CTNAG 1 cut(s) 400
BstDSI CCRYGG 2 cut(s) 154, 382
BstFNI CGCG 3 cut(s) 186, 434, 446
BstHHI GCGC 3 cut(s) 188, 395, 434
BstKTI GATC 6 cut(s) 9, 144, 161, 283, 478, 511
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 6 cut(s) 6, 141, 158, 280, 475, 508
BstMWI GCNNNNNNNGC 3 cut(s) 185, 230, 596
BstSFI CTRYAG 2 cut(s) 364, 538
BstUI CGCG 3 cut(s) 186, 434, 446
BstV1I GCAGC 1 cut(s) 122
BstV2I GAAGAC 4 cut(s) 85, 292, 346, 370
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
BstZ17I GTATAC 1 cut(s) 683
BsuRI GGCC 2 cut(s) 330, 405
BtgI CCRYGG 2 cut(s) 154, 382
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 391, 456
CfoI GCGC 3 cut(s) 188, 395, 434
Cfr13I GGNCC 1 cut(s) 563
CseI GACGC 2 cut(s) 250, 460
Csp6I GTAC 2 cut(s) 317, 505
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 3 cut(s) 55, 155, 614
CviJI RGCY 9 cut(s) 128, 135, 330, 341, 389, 405, 419, 494, 731
CviKI_1 RGCY 9 cut(s) 128, 135, 330, 341, 389, 405, 419, 494, 731
CviQI GTAC 2 cut(s) 317, 505
DdeI CTNAG 1 cut(s) 400
DpnI GATC 6 cut(s) 8, 143, 160, 282, 477, 510
DpnII GATC 6 cut(s) 6, 141, 158, 280, 475, 508
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 290, 326
EarI CTCTTC 2 cut(s) 290, 326
Eco130I CCWWGG 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 563
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 3 cut(s) 58, 158, 617
FaiI YATR 4 cut(s) 56, 156, 615, 683
FaqI GGGAC 2 cut(s) 105, 576
FatI CATG 3 cut(s) 54, 154, 613
FblI GTMKAC 2 cut(s) 682, 747
Fnu4HI GCNGC 2 cut(s) 136, 234
Fsp4HI GCNGC 2 cut(s) 136, 234
FspBI CTAG 1 cut(s) 416
GlaI GCGC 3 cut(s) 187, 394, 433
GluI GCNGC 2 cut(s) 136, 234
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 2 cut(s) 330, 405
HapII CCGG 2 cut(s) 459, 479
HgaI GACGC 2 cut(s) 250, 460
HhaI GCGC 3 cut(s) 188, 395, 434
Hin1II CATG 3 cut(s) 58, 158, 617
Hin6I GCGC 3 cut(s) 186, 393, 432
HinP1I GCGC 3 cut(s) 186, 393, 432
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 4 cut(s) 369, 412, 482, 697
HpaII CCGG 2 cut(s) 459, 479
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 4 cut(s) 450, 505, 683, 748
Hpy188I TCNGA 4 cut(s) 17, 205, 441, 743
Hpy188III TCNNGA 7 cut(s) 32, 122, 409, 479, 486, 577, 703
Hpy8I GTNNAC 4 cut(s) 450, 505, 683, 748
Hpy99I CGWCG 2 cut(s) 266, 317
HpyAV CCTTC 3 cut(s) 47, 223, 544
HpyCH4III ACNGT 3 cut(s) 247, 542, 676
HpyCH4IV ACGT 2 cut(s) 315, 336
HpyCH4V TGCA 1 cut(s) 366
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 230, 596
HpyF3I CTNAG 1 cut(s) 400
HpySE526I ACGT 2 cut(s) 315, 336
Hsp92II CATG 3 cut(s) 58, 158, 617
HspAI GCGC 3 cut(s) 186, 393, 432
Kpn2I TCCGGA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 6, 141, 158, 280, 475, 508
LmnI GCTCC 2 cut(s) 215, 461
LpnPI CCDG 8 cut(s) 45, 152, 177, 376, 422, 472, 492, 688
Lsp1109I GCAGC 1 cut(s) 122
MaeI CTAG 1 cut(s) 416
MaeII ACGT 2 cut(s) 315, 336
MaeIII GTNAC 1 cut(s) 424
MalI GATC 6 cut(s) 8, 143, 160, 282, 477, 510
MboI GATC 6 cut(s) 6, 141, 158, 280, 475, 508
MfeI CAATTG 2 cut(s) 100, 651
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 1 cut(s) 613
MluCI AATT 7 cut(s) 27, 100, 628, 651, 663, 710, 756
MluI ACGCGT 1 cut(s) 444
MmeI TCCRAC 1 cut(s) 640
MroI TCCGGA 1 cut(s) 478
MseI TTAA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 2 cut(s) 459, 479
MunI CAATTG 2 cut(s) 100, 651
MvnI CGCG 3 cut(s) 186, 434, 446
MwoI GCNNNNNNNGC 3 cut(s) 185, 230, 596
NcoI CCATGG 1 cut(s) 154
NdeII GATC 6 cut(s) 6, 141, 158, 280, 475, 508
NlaIII CATG 3 cut(s) 58, 158, 617
NlaIV GGNNCC 1 cut(s) 564
PaeR7I CTCGAG 1 cut(s) 600
PceI AGGCCT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 333
PfeI GAWTC 4 cut(s) 369, 412, 482, 697
PkrI GCNGC 2 cut(s) 137, 235
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 563
Psp5II RGGWCCY 1 cut(s) 563
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 1 cut(s) 563
PspPPI RGGWCCY 1 cut(s) 563
PspXI VCTCGAGB 1 cut(s) 600
PstI CTGCAG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 158
RsaI GTAC 2 cut(s) 318, 506
RsaNI GTAC 2 cut(s) 317, 505
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 2 cut(s) 136, 234
Sau3AI GATC 6 cut(s) 6, 141, 158, 280, 475, 508
Sau96I GGNCC 1 cut(s) 563
SduI GDGCHC 1 cut(s) 613
SfcI CTRYAG 2 cut(s) 364, 538
Sfr274I CTCGAG 1 cut(s) 600
SinI GGWCC 1 cut(s) 563
SlaI CTCGAG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
Sse9I AATT 7 cut(s) 27, 100, 628, 651, 663, 710, 756
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 5 cut(s) 97, 167, 222, 231, 234
SspMI CTAG 1 cut(s) 416
StuI AGGCCT 1 cut(s) 405
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 247, 542, 676
TaiI ACGT 2 cut(s) 318, 339
TaqI TCGA 3 cut(s) 5, 252, 601
TasI AATT 7 cut(s) 27, 100, 628, 651, 663, 710, 756
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 369, 412, 482, 697
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 2 cut(s) 76, 313
TspGWI ACGGA 2 cut(s) 107, 513
VpaK11BI GGWCC 1 cut(s) 563
XhoI CTCGAG 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 682, 747
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.