MD17G1016700.v1.1

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004398
Physical Location & Seq
Forward (+)
1337441 .. 1338286
846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1016700.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGAAACCCAAAACCCAATCCACTCCCCGCCGTCGCCGTCGCTGTCGCCGTTGTCATCTCTTCAATTCCCATGCCTCCCCATCTTCTTCCTCATGTTCTCCATCGTCTACCTCATTGCCTACTTCATCGTCTTCCGAACTTGGAGCCCTAAGATCCGACCCGAAGCCTCCAGCTGCCTAATCTCCCTCGCCCACGGCACCCCCGCCGTCGTTCTCTCCACGTACGCCATCCTCTCCGACCCCGCCACCGGATTCGCCGCCCCCAACACCCTTTTCCAGAACTCCGTCCTCGATTACAGCGTCGCCTACTTCCTCACCGATCTCCTCCACTACCTCGTCTTCTTCCCCAGCGACGTCCTCTTCATCGGCCACCACCTCGCCACGCTCTTCGTCTTCCTAACCTGCCGCTACGTCGCCTCCCACGGTGCATTCGCCATTCTCTCCCTCCTGATCCTCGCCGAGGTCACCAGCCTCTGCCAGAACGTCTGGACGCTCGCCAACGCCAGGAGAAGCGACTTGAAGTTTGCAGCTAAGGTGTATGATCTATTGTCTCCTCCTTTTTACATTCTGTATTCGATTGTTCGTGGCTTTGTGGGTCCGTATTTCGTGTACCGAATGGGGGCCTTTTACATCAGCGGCGCCGCCGACGGATTGATTCCGCGGTGGGTTTGGGGTTCCTGGATTGTGGTAGTGATGGCCGCCATTTCTGTCAGCATTTTGTGGATTTCGAATCTCTGGGTCGAATTGTTTAGAGCCAGGACTGGCGAATTGGAAAAGAAAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

263

Amino Acids

29.34

Weight (kDa)

8.66

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 57 - 246 1.6e-17 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 197, 638
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 661
AclWI GGATC 2 cut(s) 148, 445
AcoI YGGCCR 2 cut(s) 367, 696
AcyI GRCGYC 2 cut(s) 354, 639
AfaI GTAC 2 cut(s) 224, 611
AfiI CCNNNNNNNGG 4 cut(s) 248, 460, 504, 619
AgsI TTSAA 2 cut(s) 65, 520
AjnI CCWGG 3 cut(s) 503, 677, 755
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 3 cut(s) 174, 530, 782
AluI AGCT 3 cut(s) 174, 530, 782
Alw26I GTCTC 1 cut(s) 555
AlwI GGATC 2 cut(s) 148, 445
AlwNI CAGNNNCTG 1 cut(s) 474
AoxI GGCC 3 cut(s) 367, 621, 696
ApeKI GCWGC 2 cut(s) 174, 527
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 2 cut(s) 596, 621
AsuHPI GGTGA 2 cut(s) 307, 457
AsuII TTCGAA 1 cut(s) 728
AvaII GGWCC 1 cut(s) 596
BanI GGYRCC 2 cut(s) 197, 638
BanII GRGCYC 1 cut(s) 149
BbsI GAAGAC 3 cut(s) 124, 331, 385
BbvI GCAGC 2 cut(s) 161, 539
BccI CCATC 4 cut(s) 89, 110, 236, 688
BceAI ACGGC 5 cut(s) 16, 22, 34, 191, 211
BciT130I CCWGG 3 cut(s) 505, 679, 757
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 642
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 7 cut(s) 175, 258, 406, 528, 637, 642, 699
BlsI GCNGC 7 cut(s) 176, 259, 407, 529, 638, 643, 700
Bme1390I CCNGG 3 cut(s) 505, 679, 757
Bme18I GGWCC 1 cut(s) 596
BmgT120I GGNCC 2 cut(s) 596, 621
BmiI GGNNCC 6 cut(s) 146, 199, 597, 622, 640, 676
BmrFI CCNGG 3 cut(s) 505, 679, 757
BpiI GAAGAC 3 cut(s) 124, 331, 385
BpmI CTGGAG 1 cut(s) 154
Bpu10I CCTNAGC 1 cut(s) 531
Bpu14I TTCGAA 1 cut(s) 728
BsaAI YACGTR 1 cut(s) 222
BsaHI GRCGYC 2 cut(s) 354, 639
BsaJI CCNNGG 4 cut(s) 193, 421, 459, 659
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 460, 504, 619
Bse1I ACTGG 1 cut(s) 766
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 3 cut(s) 505, 679, 757
BseDI CCNNGG 4 cut(s) 193, 421, 459, 659
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 4 cut(s) 248, 460, 504, 619
BseMI GCAATG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 766
BseRI GAGGAG 2 cut(s) 314, 543
BseXI GCAGC 2 cut(s) 161, 539
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 661
BshFI GGCC 3 cut(s) 369, 623, 698
BshNI GGYRCC 2 cut(s) 197, 638
BsiSI CCGG 1 cut(s) 249
BsiWI CGTACG 1 cut(s) 222
BslI CCNNNNNNNGG 4 cut(s) 248, 460, 504, 619
BsmAI GTCTC 1 cut(s) 555
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 3 cut(s) 369, 623, 698
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 4 cut(s) 153, 319, 450, 541
BspANI GGCC 3 cut(s) 369, 623, 698
BspFNI CGCG 1 cut(s) 661
BspLI GGNNCC 6 cut(s) 146, 199, 597, 622, 640, 676
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 2 cut(s) 148, 445
BspQI GCTCTTC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 2 cut(s) 197, 638
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 1 cut(s) 766
BssECI CCNNGG 4 cut(s) 193, 421, 459, 659
BssMI GATC 4 cut(s) 153, 319, 450, 541
BssNI GRCGYC 2 cut(s) 354, 639
Bst2UI CCWGG 3 cut(s) 505, 679, 757
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 3 cut(s) 66, 365, 392
BstACI GRCGYC 2 cut(s) 354, 639
BstBAI YACGTR 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 2 cut(s) 150, 531
BstDSI CCRYGG 3 cut(s) 193, 421, 659
BstEII GGTNACC 1 cut(s) 463
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 661
BstH2I RGCGCY 1 cut(s) 642
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 4 cut(s) 156, 322, 453, 544
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 4 cut(s) 153, 319, 450, 541
BstNI CCWGG 3 cut(s) 505, 679, 757
BstPI GGTNACC 1 cut(s) 463
BstSCI CCNGG 3 cut(s) 503, 677, 755
BstUI CGCG 1 cut(s) 661
BstV1I GCAGC 2 cut(s) 161, 539
BstV2I GAAGAC 3 cut(s) 124, 331, 385
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 3 cut(s) 369, 623, 698
BtgI CCRYGG 3 cut(s) 193, 421, 659
BtsCI GGATG 1 cut(s) 228
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 1 cut(s) 495
CaiI CAGNNNCTG 1 cut(s) 474
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 2 cut(s) 596, 621
Cfr42I CCGCGG 1 cut(s) 662
CseI GACGC 2 cut(s) 289, 499
Csp6I GTAC 2 cut(s) 223, 610
CviAII CATG 2 cut(s) 72, 94
CviQI GTAC 2 cut(s) 223, 610
DdeI CTNAG 2 cut(s) 150, 531
DinI GGCGCC 1 cut(s) 640
DpnI GATC 4 cut(s) 155, 321, 452, 543
DpnII GATC 4 cut(s) 153, 319, 450, 541
EaeI YGGCCR 2 cut(s) 367, 696
Eam1104I CTCTTC 3 cut(s) 66, 365, 392
EarI CTCTTC 3 cut(s) 66, 365, 392
Eco24I GRGCYC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 596
Eco91I GGTNACC 1 cut(s) 463
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 621
EcoO65I GGTNACC 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 503, 677, 755
EcoT38I GRGCYC 1 cut(s) 149
EgeI GGCGCC 1 cut(s) 640
EheI GGCGCC 1 cut(s) 640
FaeI CATG 2 cut(s) 75, 97
FaiI YATR 3 cut(s) 73, 95, 540
FatI CATG 2 cut(s) 71, 93
FauI CCCGC 3 cut(s) 36, 211, 250
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 7 cut(s) 175, 258, 406, 528, 637, 642, 699
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 7 cut(s) 175, 258, 406, 528, 637, 642, 699
FspBI CTAG 1 cut(s) 783
GlaI GCGC 1 cut(s) 640
GluI GCNGC 7 cut(s) 175, 258, 406, 528, 637, 642, 699
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 154
HaeII RGCGCY 1 cut(s) 642
HaeIII GGCC 3 cut(s) 369, 623, 698
HapII CCGG 1 cut(s) 249
HgaI GACGC 2 cut(s) 289, 499
HhaI GCGC 1 cut(s) 641
Hin1I GRCGYC 2 cut(s) 354, 639
Hin1II CATG 2 cut(s) 75, 97
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HinfI GANTC 3 cut(s) 252, 655, 730
HpaII CCGG 1 cut(s) 249
HphI GGTGA 2 cut(s) 307, 457
Hpy166II GTNNAC 2 cut(s) 109, 610
Hpy188I TCNGA 3 cut(s) 137, 158, 238
Hpy188III TCNNGA 3 cut(s) 277, 448, 487
Hpy8I GTNNAC 2 cut(s) 109, 610
Hpy99I CGWCG 7 cut(s) 37, 43, 212, 305, 356, 416, 650
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 4 cut(s) 221, 354, 411, 483
HpyCH4V TGCA 2 cut(s) 428, 527
HpyF3I CTNAG 2 cut(s) 150, 531
HpySE526I ACGT 4 cut(s) 221, 354, 411, 483
Hsp92I GRCGYC 2 cut(s) 354, 639
Hsp92II CATG 2 cut(s) 75, 97
HspAI GCGC 1 cut(s) 639
KasI GGCGCC 1 cut(s) 638
KspI CCGCGG 1 cut(s) 662
Kzo9I GATC 4 cut(s) 153, 319, 450, 541
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 1 cut(s) 144
Lsp1109I GCAGC 2 cut(s) 161, 539
MaeI CTAG 1 cut(s) 783
MaeII ACGT 4 cut(s) 221, 354, 411, 483
MaeIII GTNAC 1 cut(s) 463
MalI GATC 4 cut(s) 155, 321, 452, 543
MboI GATC 4 cut(s) 153, 319, 450, 541
MboII GAAGA 9 cut(s) 53, 76, 79, 124, 331, 334, 352, 379, 385
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 3 cut(s) 65, 743, 767
Mly113I GGCGCC 1 cut(s) 639
MmeI TCCRAC 2 cut(s) 181, 261
MspA1I CMGCKG 3 cut(s) 174, 636, 661
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 3 cut(s) 505, 679, 757
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 3 cut(s) 505, 679, 757
MvnI CGCG 1 cut(s) 661
NarI GGCGCC 1 cut(s) 639
NdeII GATC 4 cut(s) 153, 319, 450, 541
NlaIII CATG 2 cut(s) 75, 97
NlaIV GGNNCC 6 cut(s) 146, 199, 597, 622, 640, 676
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 463
NspV TTCGAA 1 cut(s) 728
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 297
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 3 cut(s) 252, 655, 730
Pfl23II CGTACG 1 cut(s) 222
PfoI TCCNGGA 1 cut(s) 677
PkrI GCNGC 7 cut(s) 176, 259, 407, 529, 638, 643, 700
PluTI GGCGCC 1 cut(s) 642
Ppu21I YACGTR 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 503, 677, 755
PspEI GGTNACC 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 3 cut(s) 503, 677, 755
PspLI CGTACG 1 cut(s) 222
PspN4I GGNNCC 6 cut(s) 146, 199, 597, 622, 640, 676
PspPI GGNCC 2 cut(s) 596, 621
PstNI CAGNNNCTG 1 cut(s) 474
PsuI RGATCY 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 174
RsaI GTAC 2 cut(s) 224, 611
RsaNI GTAC 2 cut(s) 223, 610
SacII CCGCGG 1 cut(s) 662
SapI GCTCTTC 1 cut(s) 392
SatI GCNGC 7 cut(s) 175, 258, 406, 528, 637, 642, 699
Sau3AI GATC 4 cut(s) 153, 319, 450, 541
Sau96I GGNCC 2 cut(s) 596, 621
ScrFI CCNGG 3 cut(s) 505, 679, 757
SduI GDGCHC 1 cut(s) 149
SfoI GGCGCC 1 cut(s) 640
Sfr303I CCGCGG 1 cut(s) 662
SfuI TTCGAA 1 cut(s) 728
SgrBI CCGCGG 1 cut(s) 662
SinI GGWCC 1 cut(s) 596
Sse9I AATT 3 cut(s) 65, 743, 767
SspDI GGCGCC 1 cut(s) 638
SspMI CTAG 1 cut(s) 783
StyD4I CCNGG 3 cut(s) 503, 677, 755
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 4 cut(s) 224, 357, 414, 486
TaqI TCGA 4 cut(s) 291, 575, 728, 741
TasI AATT 3 cut(s) 65, 743, 767
TauI GCSGC 5 cut(s) 260, 408, 639, 644, 701
TfiI GAWTC 3 cut(s) 252, 655, 730
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 2 cut(s) 174, 527
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 2 cut(s) 115, 352
TspGWI ACGGA 3 cut(s) 274, 588, 663
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 1 cut(s) 783
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.